AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01054

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01054.t1 MTR_2g014260 93.897 639 25 4 1 634 263 892 0.0 1198
MS.gene01054.t1 MTR_2g014260 93.897 639 25 4 1 634 262 891 0.0 1198
MS.gene01054.t1 MTR_3g095960 45.455 88 48 0 543 630 902 989 4.49e-17 85.9
MS.gene01054.t1 MTR_3g095960 45.455 88 48 0 543 630 933 1020 4.81e-17 85.9
MS.gene01054.t1 MTR_8g093480 33.333 132 81 2 503 630 647 775 4.67e-15 79.3
MS.gene01054.t1 MTR_8g093480 33.333 132 81 2 503 630 604 732 5.16e-15 79.0
MS.gene01054.t1 MTR_2g060650 40.698 86 51 0 548 633 1362 1447 8.97e-15 79.0
MS.gene01054.t1 MTR_8g093480 32.283 127 79 2 503 625 647 770 3.59e-13 73.2
MS.gene01054.t1 MTR_8g093480 32.283 127 79 2 503 625 604 727 4.01e-13 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01054.t1 AT2G33835 30.192 520 265 19 183 633 96 586 6.71e-43 163
MS.gene01054.t1 AT3G18640 44.578 83 46 0 548 630 590 672 1.68e-16 84.0
MS.gene01054.t1 AT3G18640 44.578 83 46 0 548 630 590 672 1.68e-16 84.0
MS.gene01054.t1 AT3G18640 44.578 83 46 0 548 630 590 672 1.68e-16 84.0
MS.gene01054.t1 AT3G26850 42.353 85 49 0 550 634 181 265 2.38e-16 80.1
MS.gene01054.t1 AT3G26850 42.353 85 49 0 550 634 181 265 2.38e-16 80.1
MS.gene01054.t1 AT2G10553 42.683 82 47 0 553 634 1 82 1.64e-15 72.4

Find 126 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGAAAACCCGTTCTAAT+TGG 0.146947 2.4:+70626116 MS.gene01054:CDS
AACGTTGATGTTGAAAATTC+TGG 0.195896 2.4:+70624943 MS.gene01054:CDS
AGAGACCCATCTCCAATATT+TGG 0.208217 2.4:-70626238 None:intergenic
AAGAGTTGCTAAAACCATTT+TGG 0.214650 2.4:+70626734 MS.gene01054:CDS
CTGAAGAGAAAGAGGATTTA+TGG 0.227339 2.4:-70626033 None:intergenic
CCACACCTATGTCACCATTT+TGG 0.265214 2.4:-70626492 None:intergenic
TAATGCGATGGCCGTACTTC+AGG 0.268705 2.4:-70624757 None:intergenic
ACCTTTAGGTGCAAGTGCTT+TGG 0.308934 2.4:+70626000 MS.gene01054:CDS
TATCTTCAGTGGTCGGAATT+TGG 0.312793 2.4:-70626840 None:intergenic
AGAGAATTGGAATTGGAAAA+AGG 0.326337 2.4:+70625562 MS.gene01054:CDS
TCCTGATGTTATCTCTGCTT+TGG 0.335129 2.4:-70626455 None:intergenic
TTAAATGCAGATTTATTCTC+TGG 0.353007 2.4:-70625857 None:intergenic
AAAGCTTCCCTTCTAATTAG+AGG 0.355973 2.4:+70626262 MS.gene01054:CDS
AATTAGAGGGTCATCCGTTC+AGG 0.358517 2.4:+70626276 MS.gene01054:CDS
AGACGACCCTCATGCCAAAA+TGG 0.368470 2.4:-70626748 None:intergenic
ATGCCTTTGGTGTTGAAGAT+AGG 0.370980 2.4:+70626521 MS.gene01054:CDS
AGCATTGAGATACCAAATAT+TGG 0.373167 2.4:+70626226 MS.gene01054:CDS
AGTACGGCCATCGCATTATA+TGG 0.373759 2.4:+70624762 MS.gene01054:CDS
GGATAATAAATTTCATGAAA+TGG 0.373961 2.4:+70626627 MS.gene01054:CDS
ACGGGAAAAGATGTGGTTAC+TGG 0.386220 2.4:+70625066 MS.gene01054:CDS
TGGCCGTACTTCAGGGTTCT+CGG 0.390696 2.4:-70624749 None:intergenic
TCAGAAGAGAGAATTGGAAT+TGG 0.391834 2.4:+70625555 MS.gene01054:CDS
CGAGGAACAGAACTTCTGTT+TGG 0.394825 2.4:-70626412 None:intergenic
CGGATGACCCTCTAATTAGA+AGG 0.396020 2.4:-70626270 None:intergenic
GAAGCTTTGCTATTGCAATC+TGG 0.406483 2.4:-70625003 None:intergenic
TGAAGAGAAAGAGGATTTAT+GGG 0.410865 2.4:-70626032 None:intergenic
AAGAGGGTCATAAAGATCTC+TGG 0.414544 2.4:-70626197 None:intergenic
ACTGAAAACCCGTTCTAATT+GGG 0.418464 2.4:+70626117 MS.gene01054:CDS
AGTTGTGCAAACTACCAAAA+TGG 0.421565 2.4:+70626478 MS.gene01054:CDS
CTACCATGACCAGACCTTGA+TGG 0.426349 2.4:-70625195 None:intergenic
TTTCCCTGTAACAGGTGTTA+AGG 0.435675 2.4:+70625652 MS.gene01054:intron
AACTTGATTTCTGCTGAAGA+TGG 0.439277 2.4:+70624904 MS.gene01054:CDS
TCCATCTTTAATCTCAGCAC+TGG 0.439675 2.4:-70625395 None:intergenic
AGGGTTGCACCACATGCTAA+TGG 0.439793 2.4:+70626088 MS.gene01054:CDS
TACACATCAGAAGAGAGAAT+TGG 0.442424 2.4:+70625549 MS.gene01054:CDS
ATCCTCTTTCTCTTCAGATC+GGG 0.443545 2.4:+70626039 MS.gene01054:CDS
AGCAATATGTTTCTTTATGT+CGG 0.448405 2.4:+70626869 MS.gene01054:CDS
TTCATCTAGTTGGTTCATCT+AGG 0.451325 2.4:+70626068 MS.gene01054:CDS
TCATCTAGTTGGTTCATCTA+GGG 0.454338 2.4:+70626069 MS.gene01054:CDS
TGATTGCATAACTTTCTCCT+TGG 0.455572 2.4:-70625139 None:intergenic
TCATGAGATGGATGCCAACT+TGG 0.456645 2.4:-70625800 None:intergenic
AGAAGGTTCCCAATTAGAAC+GGG 0.465289 2.4:-70626125 None:intergenic
GGCCGTACTTCAGGGTTCTC+GGG 0.467045 2.4:-70624748 None:intergenic
CACCAAGTCATCTAGTGATA+CGG 0.475093 2.4:+70625047 MS.gene01054:CDS
TGATGTGAAAGAGGAGAACT+TGG 0.477135 2.4:+70624708 MS.gene01054:CDS
CCCAGTGCTGAGATTAAAGA+TGG 0.477210 2.4:+70625394 MS.gene01054:CDS
CTCTGCACGGGATGAATATG+AGG 0.483485 2.4:-70625912 None:intergenic
GAGATACCAAATATTGGAGA+TGG 0.486987 2.4:+70626232 MS.gene01054:CDS
AATGCGATGGCCGTACTTCA+GGG 0.489558 2.4:-70624756 None:intergenic
ACTTGATTTCTGCTGAAGAT+GGG 0.490752 2.4:+70624905 MS.gene01054:CDS
TGGTTCACTGCATCCTTCAG+TGG 0.492124 2.4:-70624983 None:intergenic
TTCCCGATCTGAAGAGAAAG+AGG 0.494946 2.4:-70626041 None:intergenic
TCCCGTATCACTAGATGACT+TGG 0.504697 2.4:-70625049 None:intergenic
TATGATGATTCTGCCGCAGT+TGG 0.505901 2.4:+70626319 MS.gene01054:CDS
CCATCTTTAATCTCAGCACT+GGG 0.507231 2.4:-70625394 None:intergenic
TATATCCTCTGTACATATGT+TGG 0.511338 2.4:-70625955 None:intergenic
AGAGGGTCATAAAGATCTCT+GGG 0.511884 2.4:-70626196 None:intergenic
TCCAAAGCACTTGCACCTAA+AGG 0.515626 2.4:-70626001 None:intergenic
AATCCTCTTTCTCTTCAGAT+CGG 0.517995 2.4:+70626038 MS.gene01054:CDS
GAAAGTTATGCAATCAGAGA+TGG 0.522327 2.4:+70625146 MS.gene01054:CDS
ATGATGATTCTGCCGCAGTT+GGG 0.531248 2.4:+70626320 MS.gene01054:CDS
TCCTCAACAAAATTATCTCT+CGG 0.531272 2.4:-70625096 None:intergenic
ATGTGAAACAAAAGCCATCA+AGG 0.533118 2.4:+70625181 MS.gene01054:CDS
ACCAAGTCATCTAGTGATAC+GGG 0.535354 2.4:+70625048 MS.gene01054:CDS
ACCAAAGCAGAGATAACATC+AGG 0.539299 2.4:+70626454 MS.gene01054:CDS
ATAGTGTGAATAATACTGAC+CGG 0.541166 2.4:+70625503 MS.gene01054:CDS
TATGGAGCTTGATGTGAAAG+AGG 0.541245 2.4:+70624699 None:intergenic
TGCCCGAGAACCCTGAAGTA+CGG 0.541918 2.4:+70624746 MS.gene01054:CDS
AGCTTCTTTATCTGATGTCA+AGG 0.546038 2.4:+70625020 MS.gene01054:CDS
AGCAATCATTTCCTCTGCAC+GGG 0.546220 2.4:-70625924 None:intergenic
GGAAACAAGCTGTCCCTCAT+CGG 0.553716 2.4:-70625836 None:intergenic
AAGCTTCCCTTCTAATTAGA+GGG 0.556317 2.4:+70626263 MS.gene01054:CDS
CGTATCACTAGATGACTTGG+TGG 0.557776 2.4:-70625046 None:intergenic
TAGCAATCATTTCCTCTGCA+CGG 0.558599 2.4:-70625925 None:intergenic
GGTGTGGGACAATATGCCTT+TGG 0.558890 2.4:+70626508 MS.gene01054:CDS
GAGGTTGACTTTCAGACAGA+TGG 0.560149 2.4:+70626649 MS.gene01054:CDS
TAATAAATTTCATGAAATGG+AGG 0.564842 2.4:+70626630 MS.gene01054:CDS
CAGAAGGTTCCCAATTAGAA+CGG 0.565372 2.4:-70626126 None:intergenic
AGATGCTTTACTCATGCTCA+TGG 0.570346 2.4:-70624797 None:intergenic
CTCATATTCATCCCGTGCAG+AGG 0.570912 2.4:+70625913 MS.gene01054:CDS
AAAAGAGAAGTTTCCGATGA+GGG 0.572557 2.4:+70625823 MS.gene01054:CDS
GATGGTCGAAATGGAACAGA+AGG 0.573242 2.4:-70626142 None:intergenic
AACTACCAAAATGGTGACAT+AGG 0.578382 2.4:+70626487 MS.gene01054:CDS
TTCTCTTCCATATAATGCGA+TGG 0.583632 2.4:-70624769 None:intergenic
TATCCTATCTTCAACACCAA+AGG 0.584215 2.4:-70626524 None:intergenic
CAGGGATATGTCAATAAACA+TGG 0.586684 2.4:+70627024 MS.gene01054:intron
AATGTCAATTCCTCATGCAA+CGG 0.586889 2.4:-70624823 None:intergenic
AAAATTCTGGACATCTTCAG+AGG 0.589780 2.4:+70624956 MS.gene01054:CDS
CAAGATCAACAAGGACTGCA+TGG 0.589786 2.4:-70626708 None:intergenic
TGCAATCAGAGATGGAGTCA+AGG 0.591805 2.4:+70625154 MS.gene01054:CDS
AGATACCAAATATTGGAGAT+GGG 0.592018 2.4:+70626233 MS.gene01054:CDS
GCAATCAGAGATGGAGTCAA+GGG 0.592070 2.4:+70625155 MS.gene01054:CDS
GTGTTAAGGAAAATGTTGAC+AGG 0.595611 2.4:+70625666 MS.gene01054:CDS
TGGAAGAAGCAAGTCCAAGT+TGG 0.600753 2.4:+70625786 MS.gene01054:CDS
TAGTGTGAATAATACTGACC+GGG 0.602372 2.4:+70625504 MS.gene01054:CDS
CCAAAATGGTGACATAGGTG+TGG 0.606094 2.4:+70626492 MS.gene01054:CDS
TTAGCAGTATCTTCAGTGGT+CGG 0.606678 2.4:-70626847 None:intergenic
AACTTGTGATGAAGCCTGAA+CGG 0.612804 2.4:-70626290 None:intergenic
AACGGACTGAGCATGATGCT+AGG 0.615927 2.4:+70626560 MS.gene01054:CDS
CTTCAGAGGAAAACCACTGA+AGG 0.618372 2.4:+70624970 MS.gene01054:CDS
GGATGACCCTCTAATTAGAA+GGG 0.619254 2.4:-70626269 None:intergenic
AAATGTTGACAGGTCAAGAA+CGG 0.621080 2.4:+70625676 MS.gene01054:CDS
GATGTGAAAGAGGAGAACTT+GGG 0.624477 2.4:+70624709 MS.gene01054:CDS
GCTAAAGGTTGGTGCATCCG+AGG 0.627168 2.4:+70625451 MS.gene01054:CDS
AGCATCTGTTCCGTTGCATG+AGG 0.628622 2.4:+70624813 MS.gene01054:CDS
TAGTGATACGGGAAAAGATG+TGG 0.628779 2.4:+70625059 MS.gene01054:CDS
ATCTCAATGCTATCACGAAG+AGG 0.632520 2.4:-70626214 None:intergenic
AGTGTATATCCATTAGCATG+TGG 0.634510 2.4:-70626097 None:intergenic
TTGCTTAGCAGTATCTTCAG+TGG 0.635718 2.4:-70626851 None:intergenic
TCTCAATGCTATCACGAAGA+GGG 0.642743 2.4:-70626213 None:intergenic
GCATGAGGGTCGTCTCAGCA+AGG 0.648486 2.4:+70626756 MS.gene01054:CDS
TCAGTGGTCGGAATTTGGCG+TGG 0.649706 2.4:-70626835 None:intergenic
GGAAGCTACAAGAACTACCT+CGG 0.649982 2.4:-70625468 None:intergenic
ATGTGAAAGAGGAGAACTTG+GGG 0.650828 2.4:+70624710 MS.gene01054:CDS
CAAAATGGTGACATAGGTGT+GGG 0.653939 2.4:+70626493 MS.gene01054:CDS
TTTGTTGAGGATGCTGATGG+TGG 0.658081 2.4:+70625108 MS.gene01054:CDS
AGACATGTGTTTCCCAACTG+CGG 0.664225 2.4:-70626332 None:intergenic
AGTGTGAATAATACTGACCG+GGG 0.665731 2.4:+70625505 MS.gene01054:CDS
TGATGGTGGTCAGAACTCCA+AGG 0.667489 2.4:+70625122 MS.gene01054:CDS
AACAGAAGTTCTGTTCCTCG+CGG 0.678826 2.4:+70626415 MS.gene01054:CDS
GTGTGAATAATACTGACCGG+GGG 0.679900 2.4:+70625506 MS.gene01054:CDS
CGAAACCAACATATGTACAG+AGG 0.688687 2.4:+70625950 MS.gene01054:CDS
AGGGTCATAAAGATCTCTGG+GGG 0.694830 2.4:-70626194 None:intergenic
ACTCTTTCACAAGATCAACA+AGG 0.723096 2.4:-70626717 None:intergenic
GAGGGTCATAAAGATCTCTG+GGG 0.728908 2.4:-70626195 None:intergenic
TGTGAATAATACTGACCGGG+GGG 0.742273 2.4:+70625507 MS.gene01054:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCATAAAACTTATGAT+GGG - chr2.4:70626389-70626408 None:intergenic 15.0%
!! GGATAATAAATTTCATGAAA+TGG + chr2.4:70626627-70626646 MS.gene01054:CDS 20.0%
!! TAATAAATTTCATGAAATGG+AGG + chr2.4:70626630-70626649 MS.gene01054:CDS 20.0%
!!! ATGCAGTGTAAATTAAATTT+TGG - chr2.4:70625256-70625275 None:intergenic 20.0%
!!! CATTTTCATAAAACTTATGA+TGG - chr2.4:70626390-70626409 None:intergenic 20.0%
!!! GGTATATCAAATAATTTGTT+TGG + chr2.4:70625583-70625602 MS.gene01054:intron 20.0%
! AACAAAAACTGTGCAAAAAA+AGG - chr2.4:70626927-70626946 None:intergenic 25.0%
! AGCAATATGTTTCTTTATGT+CGG + chr2.4:70626869-70626888 MS.gene01054:CDS 25.0%
! AGTTAAAAAATCAGTTGACA+AGG + chr2.4:70626795-70626814 MS.gene01054:CDS 25.0%
! ATTCATCGAAGTGATAAAAA+AGG - chr2.4:70626167-70626186 None:intergenic 25.0%
! CAAAAAAAATAGAGTAGCAA+AGG + chr2.4:70626606-70626625 MS.gene01054:CDS 25.0%
! TTAAATGCAGATTTATTCTC+TGG - chr2.4:70625860-70625879 None:intergenic 25.0%
! TTATTAGAAATATGCGCAAA+GGG - chr2.4:70625627-70625646 None:intergenic 25.0%
! TTTATTAGAAATATGCGCAA+AGG - chr2.4:70625628-70625647 None:intergenic 25.0%
!! AAAAAAGCACATATAATTGC+AGG - chr2.4:70625430-70625449 None:intergenic 25.0%
!! AATTTTCAACATCAACGTTT+AGG - chr2.4:70624942-70624961 None:intergenic 25.0%
!! TTTGCGCATATTTCTAATAA+AGG + chr2.4:70625627-70625646 MS.gene01054:intron 25.0%
!!! AAAGATTTTAGTTCACCTTT+AGG + chr2.4:70625986-70626005 MS.gene01054:CDS 25.0%
!!! ACTAGAAACTTTTTGTTTCA+GGG - chr2.4:70624866-70624885 None:intergenic 25.0%
!!! AGATATATATTTTTGTGTGC+AGG + chr2.4:70627005-70627024 MS.gene01054:intron 25.0%
!!! ATATGTGCTTTTTTTGCTAA+AGG + chr2.4:70625436-70625455 MS.gene01054:CDS 25.0%
!!! CAGTTTTTGTTATTTGTCAA+TGG + chr2.4:70626936-70626955 MS.gene01054:intron 25.0%
!!! GATATATATTTTTGTGTGCA+GGG + chr2.4:70627006-70627025 MS.gene01054:intron 25.0%
AACGTTGATGTTGAAAATTC+TGG + chr2.4:70624943-70624962 MS.gene01054:CDS 30.0%
AGAGAATTGGAATTGGAAAA+AGG + chr2.4:70625562-70625581 MS.gene01054:CDS 30.0%
AGATACCAAATATTGGAGAT+GGG + chr2.4:70626233-70626252 MS.gene01054:CDS 30.0%
ATAAAAAAGGATGGTCGAAA+TGG - chr2.4:70626154-70626173 None:intergenic 30.0%
ATAATGATAGAGACTATGTG+TGG - chr2.4:70625328-70625347 None:intergenic 30.0%
ATAGTGTGAATAATACTGAC+CGG + chr2.4:70625503-70625522 MS.gene01054:CDS 30.0%
ATCGAAGTGATAAAAAAGGA+TGG - chr2.4:70626163-70626182 None:intergenic 30.0%
CTTCTATGAAAAGCAGTAAA+TGG - chr2.4:70625716-70625735 None:intergenic 30.0%
GGACTAATTTATGTTGTTAG+AGG - chr2.4:70625307-70625326 None:intergenic 30.0%
GGATAGAAACACTGAAAAAA+CGG + chr2.4:70626542-70626561 MS.gene01054:CDS 30.0%
TATATCCTCTGTACATATGT+TGG - chr2.4:70625958-70625977 None:intergenic 30.0%
TATTAGAAATATGCGCAAAG+GGG - chr2.4:70625626-70625645 None:intergenic 30.0%
TATTCTGTCAAATCAAACGA+AGG - chr2.4:70626986-70627005 None:intergenic 30.0%
TCCTCAACAAAATTATCTCT+CGG - chr2.4:70625099-70625118 None:intergenic 30.0%
TGAAGAGAAAGAGGATTTAT+GGG - chr2.4:70626035-70626054 None:intergenic 30.0%
! AAGAGTTGCTAAAACCATTT+TGG + chr2.4:70626734-70626753 MS.gene01054:CDS 30.0%
! AGCATTGAGATACCAAATAT+TGG + chr2.4:70626226-70626245 MS.gene01054:CDS 30.0%
! ATCAAGTTTTCACTTGGTTT+TGG - chr2.4:70624892-70624911 None:intergenic 30.0%
! TCACACTATCTTTTACATGA+AGG - chr2.4:70625492-70625511 None:intergenic 30.0%
! TGGACTAACTTTGCAATTTT+TGG - chr2.4:70626895-70626914 None:intergenic 30.0%
!! AACTTCTCTTTTTCATGAGA+TGG - chr2.4:70625815-70625834 None:intergenic 30.0%
!! CTAGAAACTTTTTGTTTCAG+GGG - chr2.4:70624865-70624884 None:intergenic 30.0%
!! GACTAGAAACTTTTTGTTTC+AGG - chr2.4:70624867-70624886 None:intergenic 30.0%
AAAAAGAGAAGTTTCCGATG+AGG + chr2.4:70625822-70625841 MS.gene01054:CDS 35.0%
AAAAGAGAAGTTTCCGATGA+GGG + chr2.4:70625823-70625842 MS.gene01054:CDS 35.0%
AAAATTCTGGACATCTTCAG+AGG + chr2.4:70624956-70624975 MS.gene01054:CDS 35.0%
AAAGCTTCCCTTCTAATTAG+AGG + chr2.4:70626262-70626281 MS.gene01054:CDS 35.0%
AAATGTTGACAGGTCAAGAA+CGG + chr2.4:70625676-70625695 MS.gene01054:CDS 35.0%
AACTACCAAAATGGTGACAT+AGG + chr2.4:70626487-70626506 MS.gene01054:CDS 35.0%
AACTTGATTTCTGCTGAAGA+TGG + chr2.4:70624904-70624923 MS.gene01054:CDS 35.0%
AAGCTTCCCTTCTAATTAGA+GGG + chr2.4:70626263-70626282 MS.gene01054:CDS 35.0%
AATCCTCTTTCTCTTCAGAT+CGG + chr2.4:70626038-70626057 MS.gene01054:CDS 35.0%
AATGTCAATTCCTCATGCAA+CGG - chr2.4:70624826-70624845 None:intergenic 35.0%
ACTCTTTCACAAGATCAACA+AGG - chr2.4:70626720-70626739 None:intergenic 35.0%
ACTGAAAACCCGTTCTAATT+GGG + chr2.4:70626117-70626136 MS.gene01054:CDS 35.0%
ACTTGATTTCTGCTGAAGAT+GGG + chr2.4:70624905-70624924 MS.gene01054:CDS 35.0%
AGCTTCTTTATCTGATGTCA+AGG + chr2.4:70625020-70625039 MS.gene01054:CDS 35.0%
AGTGTATATCCATTAGCATG+TGG - chr2.4:70626100-70626119 None:intergenic 35.0%
AGTTGTGCAAACTACCAAAA+TGG + chr2.4:70626478-70626497 MS.gene01054:CDS 35.0%
ATGTGAAACAAAAGCCATCA+AGG + chr2.4:70625181-70625200 MS.gene01054:CDS 35.0%
CAGGGATATGTCAATAAACA+TGG + chr2.4:70627024-70627043 MS.gene01054:intron 35.0%
CTGAAGAGAAAGAGGATTTA+TGG - chr2.4:70626036-70626055 None:intergenic 35.0%
GAAAGTTATGCAATCAGAGA+TGG + chr2.4:70625146-70625165 MS.gene01054:CDS 35.0%
GAGATACCAAATATTGGAGA+TGG + chr2.4:70626232-70626251 MS.gene01054:CDS 35.0%
GCAAAAAAAGGACACTTACA+TGG - chr2.4:70626915-70626934 None:intergenic 35.0%
GTGTTAAGGAAAATGTTGAC+AGG + chr2.4:70625666-70625685 MS.gene01054:CDS 35.0%
TACACATCAGAAGAGAGAAT+TGG + chr2.4:70625549-70625568 MS.gene01054:CDS 35.0%
TAGTGTGAATAATACTGACC+GGG + chr2.4:70625504-70625523 MS.gene01054:CDS 35.0%
TATCCTATCTTCAACACCAA+AGG - chr2.4:70626527-70626546 None:intergenic 35.0%
TCAGAAGAGAGAATTGGAAT+TGG + chr2.4:70625555-70625574 MS.gene01054:CDS 35.0%
TGAAAAGCAGTAAATGGAAC+TGG - chr2.4:70625710-70625729 None:intergenic 35.0%
TGATTGCATAACTTTCTCCT+TGG - chr2.4:70625142-70625161 None:intergenic 35.0%
TTCTCTTCCATATAATGCGA+TGG - chr2.4:70624772-70624791 None:intergenic 35.0%
TTTCCTTAACACCTGTTACA+GGG - chr2.4:70625658-70625677 None:intergenic 35.0%
! AATTTTGTTGAGGATGCTGA+TGG + chr2.4:70625105-70625124 MS.gene01054:CDS 35.0%
! ACCGAGAGATAATTTTGTTG+AGG + chr2.4:70625095-70625114 MS.gene01054:CDS 35.0%
! GCAGAAATCAAGTTTTCACT+TGG - chr2.4:70624898-70624917 None:intergenic 35.0%
! TATTTGTCAATGGAAGTACG+TGG + chr2.4:70626946-70626965 MS.gene01054:intron 35.0%
! TCATCTAGTTGGTTCATCTA+GGG + chr2.4:70626069-70626088 MS.gene01054:CDS 35.0%
! TTCATCTAGTTGGTTCATCT+AGG + chr2.4:70626068-70626087 MS.gene01054:CDS 35.0%
! TTTTCCTTAACACCTGTTAC+AGG - chr2.4:70625659-70625678 None:intergenic 35.0%
!! CTAAAACCATTTTGGCATGA+GGG + chr2.4:70626742-70626761 MS.gene01054:CDS 35.0%
!!! AAGTTTTCACTTGGTTTTGG+AGG - chr2.4:70624889-70624908 None:intergenic 35.0%
!!! GTGCTTTTTTTGCTAAAGGT+TGG + chr2.4:70625440-70625459 MS.gene01054:CDS 35.0%
AAACAAAAGCCATCAAGGTC+TGG + chr2.4:70625186-70625205 MS.gene01054:intron 40.0%
AACTTGTGATGAAGCCTGAA+CGG - chr2.4:70626293-70626312 None:intergenic 40.0%
AAGAGGGTCATAAAGATCTC+TGG - chr2.4:70626200-70626219 None:intergenic 40.0%
ACCAAAGCAGAGATAACATC+AGG + chr2.4:70626454-70626473 MS.gene01054:CDS 40.0%
ACCAAGTCATCTAGTGATAC+GGG + chr2.4:70625048-70625067 MS.gene01054:CDS 40.0%
AGAAGGTTCCCAATTAGAAC+GGG - chr2.4:70626128-70626147 None:intergenic 40.0%
AGAGACCCATCTCCAATATT+TGG - chr2.4:70626241-70626260 None:intergenic 40.0%
AGAGGGTCATAAAGATCTCT+GGG - chr2.4:70626199-70626218 None:intergenic 40.0%
AGATGCTTTACTCATGCTCA+TGG - chr2.4:70624800-70624819 None:intergenic 40.0%
AGTGTGAATAATACTGACCG+GGG + chr2.4:70625505-70625524 MS.gene01054:CDS 40.0%
ATCCTCTTTCTCTTCAGATC+GGG + chr2.4:70626039-70626058 MS.gene01054:CDS 40.0%
ATCTCAATGCTATCACGAAG+AGG - chr2.4:70626217-70626236 None:intergenic 40.0%
ATGTGAAAGAGGAGAACTTG+GGG + chr2.4:70624710-70624729 MS.gene01054:CDS 40.0%
CAAAATGGTGACATAGGTGT+GGG + chr2.4:70626493-70626512 MS.gene01054:CDS 40.0%
CACCAAGTCATCTAGTGATA+CGG + chr2.4:70625047-70625066 MS.gene01054:CDS 40.0%
CACTGAAAACCCGTTCTAAT+TGG + chr2.4:70626116-70626135 MS.gene01054:CDS 40.0%
CAGAAGGTTCCCAATTAGAA+CGG - chr2.4:70626129-70626148 None:intergenic 40.0%
CCATCTTTAATCTCAGCACT+GGG - chr2.4:70625397-70625416 None:intergenic 40.0%
CGAAACCAACATATGTACAG+AGG + chr2.4:70625950-70625969 MS.gene01054:CDS 40.0%
GATGTGAAAGAGGAGAACTT+GGG + chr2.4:70624709-70624728 MS.gene01054:CDS 40.0%
GCTGAAGATGGGAAAAATCA+AGG + chr2.4:70624916-70624935 MS.gene01054:CDS 40.0%
GGATGACCCTCTAATTAGAA+GGG - chr2.4:70626272-70626291 None:intergenic 40.0%
TAGCAATCATTTCCTCTGCA+CGG - chr2.4:70625928-70625947 None:intergenic 40.0%
TAGTGATACGGGAAAAGATG+TGG + chr2.4:70625059-70625078 MS.gene01054:CDS 40.0%
TATCTTCAGTGGTCGGAATT+TGG - chr2.4:70626843-70626862 None:intergenic 40.0%
TCCATCTTTAATCTCAGCAC+TGG - chr2.4:70625398-70625417 None:intergenic 40.0%
TCCTGATGTTATCTCTGCTT+TGG - chr2.4:70626458-70626477 None:intergenic 40.0%
TCTCAATGCTATCACGAAGA+GGG - chr2.4:70626216-70626235 None:intergenic 40.0%
TGATGTGAAAGAGGAGAACT+TGG + chr2.4:70624708-70624727 MS.gene01054:CDS 40.0%
TTAGCAGTATCTTCAGTGGT+CGG - chr2.4:70626850-70626869 None:intergenic 40.0%
TTCTGCATGCTCATTTGCAA+AGG - chr2.4:70625764-70625783 None:intergenic 40.0%
TTGCTTAGCAGTATCTTCAG+TGG - chr2.4:70626854-70626873 None:intergenic 40.0%
TTTCCCTGTAACAGGTGTTA+AGG + chr2.4:70625652-70625671 MS.gene01054:intron 40.0%
TTTGTTTGGTCAACCTCTTC+AGG + chr2.4:70625597-70625616 MS.gene01054:intron 40.0%
! CGGGAAGATTTTCATCTAGT+TGG + chr2.4:70626058-70626077 MS.gene01054:CDS 40.0%
!! ATGCCTTTGGTGTTGAAGAT+AGG + chr2.4:70626521-70626540 MS.gene01054:CDS 40.0%
!! GAAGCTTTGCTATTGCAATC+TGG - chr2.4:70625006-70625025 None:intergenic 40.0%
!! GCTAAAACCATTTTGGCATG+AGG + chr2.4:70626741-70626760 MS.gene01054:CDS 40.0%
!! TAAAGGCATTTCCCTGTAAC+AGG + chr2.4:70625644-70625663 MS.gene01054:intron 40.0%
AACAGAAGTTCTGTTCCTCG+CGG + chr2.4:70626415-70626434 MS.gene01054:CDS 45.0%
AATTAGAGGGTCATCCGTTC+AGG + chr2.4:70626276-70626295 MS.gene01054:CDS 45.0%
ACCTTTAGGTGCAAGTGCTT+TGG + chr2.4:70626000-70626019 MS.gene01054:CDS 45.0%
ACGGGAAAAGATGTGGTTAC+TGG + chr2.4:70625066-70625085 MS.gene01054:CDS 45.0%
AGACATGTGTTTCCCAACTG+CGG - chr2.4:70626335-70626354 None:intergenic 45.0%
AGACTTCTAGTCCTGCTATC+AGG - chr2.4:70625370-70625389 None:intergenic 45.0%
AGCAATCATTTCCTCTGCAC+GGG - chr2.4:70625927-70625946 None:intergenic 45.0%
AGGGTCATAAAGATCTCTGG+GGG - chr2.4:70626197-70626216 None:intergenic 45.0%
AGTACGGCCATCGCATTATA+TGG + chr2.4:70624762-70624781 MS.gene01054:CDS 45.0%
ATGATGATTCTGCCGCAGTT+GGG + chr2.4:70626320-70626339 MS.gene01054:CDS 45.0%
CAAGATCAACAAGGACTGCA+TGG - chr2.4:70626711-70626730 None:intergenic 45.0%
CCAAAATGGTGACATAGGTG+TGG + chr2.4:70626492-70626511 MS.gene01054:CDS 45.0%
CGAGGAACAGAACTTCTGTT+TGG - chr2.4:70626415-70626434 None:intergenic 45.0%
CGGATGACCCTCTAATTAGA+AGG - chr2.4:70626273-70626292 None:intergenic 45.0%
CTTCAGAGGAAAACCACTGA+AGG + chr2.4:70624970-70624989 MS.gene01054:CDS 45.0%
GAGGGTCATAAAGATCTCTG+GGG - chr2.4:70626198-70626217 None:intergenic 45.0%
GATGGTCGAAATGGAACAGA+AGG - chr2.4:70626145-70626164 None:intergenic 45.0%
GATTAACTCCTCCTGATAGC+AGG + chr2.4:70625356-70625375 MS.gene01054:intron 45.0%
GCAAATGAGCATGCAGAATG+TGG + chr2.4:70625766-70625785 MS.gene01054:intron 45.0%
GCAATCAGAGATGGAGTCAA+GGG + chr2.4:70625155-70625174 MS.gene01054:CDS 45.0%
GGAAGCTACAAGAACTACCT+CGG - chr2.4:70625471-70625490 None:intergenic 45.0%
GTGTGAATAATACTGACCGG+GGG + chr2.4:70625506-70625525 MS.gene01054:CDS 45.0%
TATGATGATTCTGCCGCAGT+TGG + chr2.4:70626319-70626338 MS.gene01054:CDS 45.0%
TCATGAGATGGATGCCAACT+TGG - chr2.4:70625803-70625822 None:intergenic 45.0%
TCCCGTATCACTAGATGACT+TGG - chr2.4:70625052-70625071 None:intergenic 45.0%
TGCAATCAGAGATGGAGTCA+AGG + chr2.4:70625154-70625173 MS.gene01054:CDS 45.0%
TGGAAGAAGCAAGTCCAAGT+TGG + chr2.4:70625786-70625805 MS.gene01054:CDS 45.0%
TGTGAATAATACTGACCGGG+GGG + chr2.4:70625507-70625526 MS.gene01054:CDS 45.0%
TTCCCGATCTGAAGAGAAAG+AGG - chr2.4:70626044-70626063 None:intergenic 45.0%
TTTGTTGAGGATGCTGATGG+TGG + chr2.4:70625108-70625127 MS.gene01054:CDS 45.0%
! CCACACCTATGTCACCATTT+TGG - chr2.4:70626495-70626514 None:intergenic 45.0%
! CCCAGTGCTGAGATTAAAGA+TGG + chr2.4:70625394-70625413 MS.gene01054:CDS 45.0%
! CGTATCACTAGATGACTTGG+TGG - chr2.4:70625049-70625068 None:intergenic 45.0%
! GAGGTTGACTTTCAGACAGA+TGG + chr2.4:70626649-70626668 MS.gene01054:CDS 45.0%
! TCCAAAGCACTTGCACCTAA+AGG - chr2.4:70626004-70626023 None:intergenic 45.0%
AAGCCATCAAGGTCTGGTCA+TGG + chr2.4:70625192-70625211 MS.gene01054:intron 50.0%
AATGCGATGGCCGTACTTCA+GGG - chr2.4:70624759-70624778 None:intergenic 50.0%
AGACGACCCTCATGCCAAAA+TGG - chr2.4:70626751-70626770 None:intergenic 50.0%
AGCATCTGTTCCGTTGCATG+AGG + chr2.4:70624813-70624832 MS.gene01054:CDS 50.0%
AGGGTTGCACCACATGCTAA+TGG + chr2.4:70626088-70626107 MS.gene01054:CDS 50.0%
CTACCATGACCAGACCTTGA+TGG - chr2.4:70625198-70625217 None:intergenic 50.0%
CTCATATTCATCCCGTGCAG+AGG + chr2.4:70625913-70625932 MS.gene01054:CDS 50.0%
CTCTGCACGGGATGAATATG+AGG - chr2.4:70625915-70625934 None:intergenic 50.0%
CTTCTAGTCCTGCTATCAGG+AGG - chr2.4:70625367-70625386 None:intergenic 50.0%
GGAAACAAGCTGTCCCTCAT+CGG - chr2.4:70625839-70625858 None:intergenic 50.0%
GGTGTGGGACAATATGCCTT+TGG + chr2.4:70626508-70626527 MS.gene01054:CDS 50.0%
TAATGCGATGGCCGTACTTC+AGG - chr2.4:70624760-70624779 None:intergenic 50.0%
TGGTTCACTGCATCCTTCAG+TGG - chr2.4:70624986-70625005 None:intergenic 50.0%
! GCAAGACAATCTTTTCCGCG+AGG - chr2.4:70626433-70626452 None:intergenic 50.0%
!! AACGGACTGAGCATGATGCT+AGG + chr2.4:70626560-70626579 MS.gene01054:CDS 50.0%
!! TGATGGTGGTCAGAACTCCA+AGG + chr2.4:70625122-70625141 MS.gene01054:CDS 50.0%
AATACTGACCGGGGGGATGA+AGG + chr2.4:70625514-70625533 MS.gene01054:CDS 55.0%
CGCAAAGGGGATACCTGAAG+AGG - chr2.4:70625613-70625632 None:intergenic 55.0%
TCAGTGGTCGGAATTTGGCG+TGG - chr2.4:70626838-70626857 None:intergenic 55.0%
TGCCCGAGAACCCTGAAGTA+CGG + chr2.4:70624746-70624765 MS.gene01054:CDS 55.0%
TGGCCGTACTTCAGGGTTCT+CGG - chr2.4:70624752-70624771 None:intergenic 55.0%
!! GCTAAAGGTTGGTGCATCCG+AGG + chr2.4:70625451-70625470 MS.gene01054:CDS 55.0%
CTACGTCTCCTTCATCCCCC+CGG - chr2.4:70625525-70625544 None:intergenic 60.0%
GCATGAGGGTCGTCTCAGCA+AGG + chr2.4:70626756-70626775 MS.gene01054:CDS 60.0%
GGCCGTACTTCAGGGTTCTC+GGG - chr2.4:70624751-70624770 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 70624700 70627056 70624700 ID=MS.gene01054
chr2.4 mRNA 70624700 70627056 70624700 ID=MS.gene01054.t1;Parent=MS.gene01054
chr2.4 exon 70624700 70625202 70624700 ID=MS.gene01054.t1.exon1;Parent=MS.gene01054.t1
chr2.4 CDS 70624700 70625202 70624700 ID=cds.MS.gene01054.t1;Parent=MS.gene01054.t1
chr2.4 exon 70625378 70625583 70625378 ID=MS.gene01054.t1.exon2;Parent=MS.gene01054.t1
chr2.4 CDS 70625378 70625583 70625378 ID=cds.MS.gene01054.t1;Parent=MS.gene01054.t1
chr2.4 exon 70625666 70625697 70625666 ID=MS.gene01054.t1.exon3;Parent=MS.gene01054.t1
chr2.4 CDS 70625666 70625697 70625666 ID=cds.MS.gene01054.t1;Parent=MS.gene01054.t1
chr2.4 exon 70625782 70626915 70625782 ID=MS.gene01054.t1.exon4;Parent=MS.gene01054.t1
chr2.4 CDS 70625782 70626915 70625782 ID=cds.MS.gene01054.t1;Parent=MS.gene01054.t1
chr2.4 exon 70627027 70627056 70627027 ID=MS.gene01054.t1.exon5;Parent=MS.gene01054.t1
chr2.4 CDS 70627027 70627056 70627027 ID=cds.MS.gene01054.t1;Parent=MS.gene01054.t1
Gene Sequence

>MS.gene01054

ATGGAGCTTGATGTGAAAGAGGAGAACTTGGGGTCAGTTTCTTATGTGCCCGAGAACCCTGAAGTACGGCCATCGCATTATATGGAAGAGAATGATACCATGAGCATGAGTAAAGCATCTGTTCCGTTGCATGAGGAATTGACATTGAAAAATGAAGATTATCCCCTGAAACAAAAAGTTTCTAGTCCTCCAAAACCAAGTGAAAACTTGATTTCTGCTGAAGATGGGAAAAATCAAGGCCTAAACGTTGATGTTGAAAATTCTGGACATCTTCAGAGGAAAACCACTGAAGGATGCAGTGAACCAGATTGCAATAGCAAAGCTTCTTTATCTGATGTCAAGGATGCCACCAAGTCATCTAGTGATACGGGAAAAGATGTGGTTACTGGAATCATACCGAGAGATAATTTTGTTGAGGATGCTGATGGTGGTCAGAACTCCAAGGAGAAAGTTATGCAATCAGAGATGGAGTCAAGGGTTGATGTGAAACAAAAGCCATCAAGGTCTGGTCATGGTAGTTCAGTATGTTCTTTTAAGTTTATTTACAAGATGACCAAAATTTAATTTACACTGCATGCTTTCTCTTTCAGAAGTAGCTTCTTGTCCTCTAACAACATAAATTAGTCCACACATAGTCTCTATCATTATGTTTTTCTGATTAACTCCTCCTGATAGCAGGACTAGAAGTCTGTCTCCCAGTGCTGAGATTAAAGATGGAAACAAAAGACCTGCAATTATATGTGCTTTTTTTGCTAAAGGTTGGTGCATCCGAGGTAGTTCTTGTAGCTTCCTTCATGTAAAAGATAGTGTGAATAATACTGACCGGGGGGATGAAGGAGACGTAGTTACTACACATCAGAAGAGAGAATTGGAATTGGAAAAAGGTATATCAAATAATTTGTTTGGTCAACCTCTTCAGGTATCCCCTTTGCGCATATTTCTAATAAAGGCATTTCCCTGTAACAGGTGTTAAGGAAAATGTTGACAGGTCAAGAACGGTTAGTACTCCAGTTCCATTTACTGCTTTTCATAGAAGTATAACTTAACACTCTTCTGAATTTCCTTTGCAAATGAGCATGCAGAATGTGGAAGAAGCAAGTCCAAGTTGGCATCCATCTCATGAAAAAGAGAAGTTTCCGATGAGGGACAGCTTGTTTCCAGAGAATAAATCTGCATTTAATTCATCAAACAATTATTTCAGCTCAAATCTTTCCTCATATTCATCCCGTGCAGAGGAAATGATTGCTATTCGAAACCAACATATGTACAGAGGATATACATCGACAAAAGATTTTAGTTCACCTTTAGGTGCAAGTGCTTTGGACTCTCACGCCCATAAATCCTCTTTCTCTTCAGATCGGGAAGATTTTCATCTAGTTGGTTCATCTAGGGTTGCACCACATGCTAATGGATATACACTGAAAACCCGTTCTAATTGGGAACCTTCTGTTCCATTTCGACCATCCTTTTTTATCACTTCGATGAATGTATCATCCCCCAGAGATCTTTATGACCCTCTTCGTGATAGCATTGAGATACCAAATATTGGAGATGGGTCTCTGAAAGCTTCCCTTCTAATTAGAGGGTCATCCGTTCAGGCTTCATCACAAGTTCGAACATATGATGATTCTGCCGCAGTTGGGAAACACATGTCTGATGTTAATGATGATAAAAGTTCTGTATCTTCCCATCATAAGTTTTATGAAAATGAGCCAAACAGAAGTTCTGTTCCTCGCGGAAAAGATTGTCTTGCAACCAAAGCAGAGATAACATCAGGAAGTTGTGCAAACTACCAAAATGGTGACATAGGTGTGGGACAATATGCCTTTGGTGTTGAAGATAGGATAGAAACACTGAAAAAACGGACTGAGCATGATGCTAGGCATCATAGTGATGAATCAGAGCACAAAAAAAATAGAGTAGCAAAGGATAATAAATTTCATGAAATGGAGGTTGACTTTCAGACAGATGGCAGTGTACTCAAAGAAACAAAGACGCTGAAAATTTTCCATGCAGTCCTTGTTGATCTTGTGAAAGAGTTGCTAAAACCATTTTGGCATGAGGGTCGTCTCAGCAAGGATGCACATATATTGATAGTTAAAAAATCAGTTGACAAGGTTATTAGCACTTTAGAGCCACGCCAAATTCCGACCACTGAAGATACTGCTAAGCAATATGTTTCTTTATGTCGGCCAAAAATTGCAAAGTTAGTCCATGTAAGTGTCCTTTTTTTGCACAGTTTTTGTTATTTGTCAATGGAAGTACGTGGACTTTCTTGTTTGTCCTTCGTTTGATTTGACAGAATAGATATATATTTTTGTGTGCAGGGATATGTCAATAAACATGGCAAATCTTGA
Protein sequence

>MS.gene01054.t1

MELDVKEENLGSVSYVPENPEVRPSHYMEENDTMSMSKASVPLHEELTLKNEDYPLKQKVSSPPKPSENLISAEDGKNQGLNVDVENSGHLQRKTTEGCSEPDCNSKASLSDVKDATKSSSDTGKDVVTGIIPRDNFVEDADGGQNSKEKVMQSEMESRVDVKQKPSRTRSLSPSAEIKDGNKRPAIICAFFAKGWCIRGSSCSFLHVKDSVNNTDRGDEGDVVTTHQKRELELEKGVKENVDRSRTNVEEASPSWHPSHEKEKFPMRDSLFPENKSAFNSSNNYFSSNLSSYSSRAEEMIAIRNQHMYRGYTSTKDFSSPLGASALDSHAHKSSFSSDREDFHLVGSSRVAPHANGYTLKTRSNWEPSVPFRPSFFITSMNVSSPRDLYDPLRDSIEIPNIGDGSLKASLLIRGSSVQASSQVRTYDDSAAVGKHMSDVNDDKSSVSSHHKFYENEPNRSSVPRGKDCLATKAEITSGSCANYQNGDIGVGQYAFGVEDRIETLKKRTEHDARHHSDESEHKKNRVAKDNKFHEMEVDFQTDGSVLKETKTLKIFHAVLVDLVKELLKPFWHEGRLSKDAHILIVKKSVDKVISTLEPRQIPTTEDTAKQYVSLCRPKIAKLVHGYVNKHGKS