Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01070.t1 | XP_003593591.1 | 87.9 | 173 | 19 | 2 | 1 | 171 | 1 | 173 | 3.30E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01070.t1 | F4JIJ7 | 41.8 | 177 | 91 | 4 | 3 | 167 | 7 | 183 | 1.4e-26 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01070.t1 | G7IPU7 | 87.9 | 173 | 19 | 2 | 1 | 171 | 1 | 173 | 2.4e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene01070.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01070.t1 | MTR_2g013920 | 88.439 | 173 | 18 | 2 | 1 | 171 | 1 | 173 | 5.06e-107 | 303 |
| MS.gene01070.t1 | MTR_2g090440 | 42.262 | 168 | 89 | 3 | 6 | 167 | 12 | 177 | 2.14e-38 | 130 |
| MS.gene01070.t1 | MTR_2g090480 | 40.000 | 170 | 92 | 4 | 6 | 167 | 12 | 179 | 2.05e-36 | 125 |
| MS.gene01070.t1 | MTR_2g090425 | 41.143 | 175 | 92 | 4 | 6 | 171 | 12 | 184 | 5.32e-36 | 127 |
| MS.gene01070.t1 | MTR_4g078350 | 44.134 | 179 | 78 | 8 | 3 | 167 | 7 | 177 | 3.58e-35 | 122 |
| MS.gene01070.t1 | MTR_2g090500 | 36.806 | 144 | 85 | 2 | 28 | 167 | 49 | 190 | 7.35e-27 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene01070.t1 | AT4G20970 | 41.808 | 177 | 91 | 5 | 3 | 167 | 7 | 183 | 4.72e-38 | 129 |
Find 27 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATTTAGGTAGGAGAATT+TGG | 0.200977 | 2.4:-70908248 | MS.gene01070:intron |
| TTAGAGGTTGTTCTAATAAC+TGG | 0.223327 | 2.4:-70909355 | MS.gene01070:CDS |
| AAAATTAAGATACAACAAAT+TGG | 0.306857 | 2.4:-70909385 | MS.gene01070:CDS |
| TTTCTATTCATATTCACATT+TGG | 0.306963 | 2.4:+70909433 | None:intergenic |
| ACAAATTGGTTTAGTCTTAG+AGG | 0.319445 | 2.4:-70909371 | MS.gene01070:CDS |
| TCTGCCTCATCAAACTTCTA+AGG | 0.344529 | 2.4:-70909780 | MS.gene01070:intron |
| CTGTCAACTCTTGATGAACT+AGG | 0.359161 | 2.4:+70909866 | None:intergenic |
| TATTGTCAATGCAAGTTATA+AGG | 0.360092 | 2.4:-70909272 | MS.gene01070:CDS |
| GGTTGTTCTAATAACTGGAT+TGG | 0.435753 | 2.4:-70909350 | MS.gene01070:CDS |
| CTTCGTGTTCTTCATGAAGA+AGG | 0.454763 | 2.4:-70909301 | MS.gene01070:CDS |
| TTTCCATTCAATACACTGCC+AGG | 0.478671 | 2.4:-70909233 | MS.gene01070:intron |
| GAAAAGAAGAACTTTCTACT+AGG | 0.532951 | 2.4:-70909466 | MS.gene01070:CDS |
| TGTCAACTCTTGATGAACTA+GGG | 0.540967 | 2.4:+70909867 | None:intergenic |
| GATCAACTTGGAGAAAATGA+AGG | 0.550998 | 2.4:-70909488 | MS.gene01070:CDS |
| CTAGTTCATCAAGAGTTGAC+AGG | 0.559038 | 2.4:-70909865 | MS.gene01070:CDS |
| TTACCTGGCAGTGTATTGAA+TGG | 0.573100 | 2.4:+70909230 | None:intergenic |
| ACTTTCTACTAGGAATTCAA+AGG | 0.595040 | 2.4:-70909456 | MS.gene01070:CDS |
| AAAGAAATTACAGATCAACT+TGG | 0.602314 | 2.4:-70909500 | MS.gene01070:CDS |
| GAGACCTTAGAAGTTTGATG+AGG | 0.624740 | 2.4:+70909776 | None:intergenic |
| ATGAATAGAAACAAGAACAT+GGG | 0.625623 | 2.4:-70909421 | MS.gene01070:CDS |
| TATGAATAGAAACAAGAACA+TGG | 0.630359 | 2.4:-70909422 | MS.gene01070:CDS |
| TCTGATGCAGGCGATTTCAG+CGG | 0.655278 | 2.4:-70909554 | MS.gene01070:intron |
| GCAGAAATGAGTTGAGCTTG+TGG | 0.677546 | 2.4:+70909798 | None:intergenic |
| CTTCTTCATGAAGAACACGA+AGG | 0.679799 | 2.4:+70909302 | None:intergenic |
| AATTTGGTAATGAAGCTGCA+AGG | 0.690882 | 2.4:-70908232 | MS.gene01070:CDS |
| GAAATGAGTTGAGCTTGTGG+CGG | 0.691957 | 2.4:+70909801 | None:intergenic |
| TTCAGCGGCAGATCAGCTCG+AGG | 0.698732 | 2.4:-70909539 | MS.gene01070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGATAAAAATATGATTATT+TGG | - | chr2.4:70909711-70909730 | MS.gene01070:intron | 10.0% |
| !!! | TAATTAGAAGTTTTTTTTTT+TGG | + | chr2.4:70909162-70909181 | None:intergenic | 10.0% |
| !!! | TATGATTTTTATAATATTTG+TGG | - | chr2.4:70909448-70909467 | MS.gene01070:CDS | 10.0% |
| !! | AAAATTAAGATACAACAAAT+TGG | - | chr2.4:70908689-70908708 | MS.gene01070:intron | 15.0% |
| !! | AAAGTTAACTATTTATAGAT+AGG | + | chr2.4:70909791-70909810 | None:intergenic | 15.0% |
| !! | TTAATTTCCTTTAATATGAA+CGG | - | chr2.4:70908882-70908901 | MS.gene01070:intron | 15.0% |
| !!! | ATTTGTTGTATCTTAATTTT+TGG | + | chr2.4:70908689-70908708 | None:intergenic | 15.0% |
| !!! | TTTAGTTATTTTTTTCTTTG+AGG | - | chr2.4:70909335-70909354 | MS.gene01070:CDS | 15.0% |
| !!! | TTTTTTTTTTGGAAAATTTG+AGG | + | chr2.4:70909151-70909170 | None:intergenic | 15.0% |
| !! | AATTTAGTTGTCATTATAAG+TGG | - | chr2.4:70909363-70909382 | MS.gene01070:CDS | 20.0% |
| !! | ATACTTAAATACATGAATCA+GGG | + | chr2.4:70909313-70909332 | None:intergenic | 20.0% |
| !! | ATGTTAGTATTGTTAGTATT+TGG | - | chr2.4:70908481-70908500 | MS.gene01070:intron | 20.0% |
| !! | GAAAAAAATAGAATTAGTGA+CGG | - | chr2.4:70908382-70908401 | MS.gene01070:intron | 20.0% |
| !! | TTCAAACTGATATTTGAATA+CGG | - | chr2.4:70909762-70909781 | MS.gene01070:intron | 20.0% |
| !! | TTTCTATTCATATTCACATT+TGG | + | chr2.4:70908644-70908663 | None:intergenic | 20.0% |
| !!! | ATTAACTTGTGTCTTAATTA+AGG | - | chr2.4:70909050-70909069 | MS.gene01070:intron | 20.0% |
| !!! | TAATTTAATTTATCTGATGC+AGG | - | chr2.4:70908508-70908527 | MS.gene01070:intron | 20.0% |
| ! | AAAGAAATTACAGATCAACT+TGG | - | chr2.4:70908574-70908593 | MS.gene01070:intron | 25.0% |
| ! | AGTCAATAACATCAAACTAA+CGG | + | chr2.4:70909643-70909662 | None:intergenic | 25.0% |
| ! | ATGAATAGAAACAAGAACAT+GGG | - | chr2.4:70908653-70908672 | MS.gene01070:intron | 25.0% |
| ! | CATACTTAAATACATGAATC+AGG | + | chr2.4:70909314-70909333 | None:intergenic | 25.0% |
| ! | CTAATTGCATTGTTCTATTT+AGG | - | chr2.4:70909813-70909832 | MS.gene01070:CDS | 25.0% |
| ! | GTAACAATTTATGATTTACC+TGG | + | chr2.4:70908862-70908881 | None:intergenic | 25.0% |
| ! | GTTAACTATTTATAGATAGG+AGG | + | chr2.4:70909788-70909807 | None:intergenic | 25.0% |
| ! | TATGAATAGAAACAAGAACA+TGG | - | chr2.4:70908652-70908671 | MS.gene01070:intron | 25.0% |
| ! | TATTGTCAATGCAAGTTATA+AGG | - | chr2.4:70908802-70908821 | MS.gene01070:intron | 25.0% |
| !! | ATTTCTTCTTTTGCTTTCAA+TGG | + | chr2.4:70908239-70908258 | None:intergenic | 25.0% |
| !! | CATTTAGTGAATGATTTTGT+CGG | + | chr2.4:70909185-70909204 | None:intergenic | 25.0% |
| !! | TGATGTTATTGACTACAATA+TGG | - | chr2.4:70909649-70909668 | MS.gene01070:intron | 25.0% |
| ACATGATCCGTTCATATTAA+AGG | + | chr2.4:70908892-70908911 | None:intergenic | 30.0% | |
| ACTTTCTACTAGGAATTCAA+AGG | - | chr2.4:70908618-70908637 | MS.gene01070:intron | 30.0% | |
| ATATGAAATCAAGTGCAAGA+AGG | - | chr2.4:70909093-70909112 | MS.gene01070:intron | 30.0% | |
| CTTGCACTTGATTTCATATT+GGG | + | chr2.4:70909092-70909111 | None:intergenic | 30.0% | |
| GAAAAGAAGAACTTTCTACT+AGG | - | chr2.4:70908608-70908627 | MS.gene01070:intron | 30.0% | |
| GTCAATAACATCAAACTAAC+GGG | + | chr2.4:70909642-70909661 | None:intergenic | 30.0% | |
| TCAATAACATCAAACTAACG+GGG | + | chr2.4:70909641-70909660 | None:intergenic | 30.0% | |
| TCTATTTAGGTAGGAGAATT+TGG | - | chr2.4:70909826-70909845 | MS.gene01070:CDS | 30.0% | |
| TCTTGCACTTGATTTCATAT+TGG | + | chr2.4:70909093-70909112 | None:intergenic | 30.0% | |
| TTAGAGGTTGTTCTAATAAC+TGG | - | chr2.4:70908719-70908738 | MS.gene01070:intron | 30.0% | |
| TTGCATTGTTCTATTTAGGT+AGG | - | chr2.4:70909817-70909836 | MS.gene01070:CDS | 30.0% | |
| ! | CTACCGAATATTTTCTCAAA+GGG | - | chr2.4:70909601-70909620 | MS.gene01070:intron | 30.0% |
| ! | GTCTTACATACTTTTACGTA+TGG | + | chr2.4:70909216-70909235 | None:intergenic | 30.0% |
| ! | TACCCCTTTGAGAAAATATT+CGG | + | chr2.4:70909607-70909626 | None:intergenic | 30.0% |
| ! | TACCGAATATTTTCTCAAAG+GGG | - | chr2.4:70909602-70909621 | MS.gene01070:intron | 30.0% |
| ! | TCTACCGAATATTTTCTCAA+AGG | - | chr2.4:70909600-70909619 | MS.gene01070:intron | 30.0% |
| !! | ACAAATTGGTTTAGTCTTAG+AGG | - | chr2.4:70908703-70908722 | MS.gene01070:intron | 30.0% |
| !! | GTCAATTATGCTTTGTTTCT+AGG | - | chr2.4:70909248-70909267 | MS.gene01070:CDS | 30.0% |
| !! | TTCCTTAACTTGTGTTCTTA+AGG | + | chr2.4:70908924-70908943 | None:intergenic | 30.0% |
| !!! | GTCACTATGTTTTAAAACGA+CGG | - | chr2.4:70908458-70908477 | MS.gene01070:intron | 30.0% |
| CTAAGAGAAGAAGAGAGTAA+AGG | + | chr2.4:70908333-70908352 | None:intergenic | 35.0% | |
| GATCAACTTGGAGAAAATGA+AGG | - | chr2.4:70908586-70908605 | MS.gene01070:intron | 35.0% | |
| GGTTGTTCTAATAACTGGAT+TGG | - | chr2.4:70908724-70908743 | MS.gene01070:intron | 35.0% | |
| TGCCTTAAGAACACAAGTTA+AGG | - | chr2.4:70908919-70908938 | MS.gene01070:intron | 35.0% | |
| TGTCAACTCTTGATGAACTA+GGG | + | chr2.4:70908210-70908229 | None:intergenic | 35.0% | |
| ! | CAAGCTTTAGTTTCACCTAA+GGG | + | chr2.4:70909288-70909307 | None:intergenic | 35.0% |
| ! | TCAAGCTTTAGTTTCACCTA+AGG | + | chr2.4:70909289-70909308 | None:intergenic | 35.0% |
| !! | AATTTGGTAATGAAGCTGCA+AGG | - | chr2.4:70909842-70909861 | MS.gene01070:CDS | 35.0% |
| ACATGACCTCGAAGTTATAG+GGG | - | chr2.4:70909124-70909143 | MS.gene01070:intron | 40.0% | |
| CACATGACCTCGAAGTTATA+GGG | - | chr2.4:70909123-70909142 | MS.gene01070:intron | 40.0% | |
| CTAGTTCATCAAGAGTTGAC+AGG | - | chr2.4:70908209-70908228 | MS.gene01070:CDS | 40.0% | |
| CTGTCAACTCTTGATGAACT+AGG | + | chr2.4:70908211-70908230 | None:intergenic | 40.0% | |
| CTTCGTGTTCTTCATGAAGA+AGG | - | chr2.4:70908773-70908792 | MS.gene01070:intron | 40.0% | |
| CTTCTTCATGAAGAACACGA+AGG | + | chr2.4:70908775-70908794 | None:intergenic | 40.0% | |
| GAGACCTTAGAAGTTTGATG+AGG | + | chr2.4:70908301-70908320 | None:intergenic | 40.0% | |
| GTAAATGTCTATGTGCCCTT+AGG | - | chr2.4:70909270-70909289 | MS.gene01070:CDS | 40.0% | |
| TCTGCCTCATCAAACTTCTA+AGG | - | chr2.4:70908294-70908313 | MS.gene01070:intron | 40.0% | |
| TTACCTGGCAGTGTATTGAA+TGG | + | chr2.4:70908847-70908866 | None:intergenic | 40.0% | |
| TTTCCATTCAATACACTGCC+AGG | - | chr2.4:70908841-70908860 | MS.gene01070:intron | 40.0% | |
| ACTTCGAGGTCATGTGCTAA+AGG | + | chr2.4:70909119-70909138 | None:intergenic | 45.0% | |
| GCACATGACCTCGAAGTTAT+AGG | - | chr2.4:70909122-70909141 | MS.gene01070:intron | 45.0% | |
| ! | GAAATGAGTTGAGCTTGTGG+CGG | + | chr2.4:70908276-70908295 | None:intergenic | 45.0% |
| ! | GCAGAAATGAGTTGAGCTTG+TGG | + | chr2.4:70908279-70908298 | None:intergenic | 45.0% |
| !! | TTATTTAATAAACAAAATAA+AGG | - | chr2.4:70909537-70909556 | MS.gene01070:CDS | 5.0% |
| TCTGATGCAGGCGATTTCAG+CGG | - | chr2.4:70908520-70908539 | MS.gene01070:intron | 50.0% | |
| TGAGGTCCCCTATAACTTCG+AGG | + | chr2.4:70909133-70909152 | None:intergenic | 50.0% | |
| TTCAGCGGCAGATCAGCTCG+AGG | - | chr2.4:70908535-70908554 | MS.gene01070:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 70908196 | 70909900 | 70908196 | ID=MS.gene01070 |
| chr2.4 | mRNA | 70908196 | 70909900 | 70908196 | ID=MS.gene01070.t1;Parent=MS.gene01070 |
| chr2.4 | exon | 70909781 | 70909900 | 70909781 | ID=MS.gene01070.t1.exon1;Parent=MS.gene01070.t1 |
| chr2.4 | CDS | 70909781 | 70909900 | 70909781 | ID=cds.MS.gene01070.t1;Parent=MS.gene01070.t1 |
| chr2.4 | exon | 70909234 | 70909566 | 70909234 | ID=MS.gene01070.t1.exon2;Parent=MS.gene01070.t1 |
| chr2.4 | CDS | 70909234 | 70909566 | 70909234 | ID=cds.MS.gene01070.t1;Parent=MS.gene01070.t1 |
| chr2.4 | exon | 70908196 | 70908261 | 70908196 | ID=MS.gene01070.t1.exon3;Parent=MS.gene01070.t1 |
| chr2.4 | CDS | 70908196 | 70908261 | 70908196 | ID=cds.MS.gene01070.t1;Parent=MS.gene01070.t1 |
| Gene Sequence |
| Protein sequence |
