Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010738.t1 | RHN45395.1 | 94.9 | 175 | 9 | 0 | 1 | 175 | 73 | 247 | 2.10E-90 | 341.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010738.t1 | A0A072V7G2 | 94.9 | 175 | 9 | 0 | 1 | 175 | 166 | 340 | 1.5e-90 | 341.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene010738.t1 | TF | PLATZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010738.t1 | MTR_2g055210 | 94.857 | 175 | 9 | 0 | 1 | 175 | 166 | 340 | 4.60e-120 | 343 |
| MS.gene010738.t1 | MTR_4g116300 | 33.333 | 183 | 110 | 4 | 1 | 174 | 70 | 249 | 1.21e-22 | 91.3 |
| MS.gene010738.t1 | MTR_4g094680 | 33.702 | 181 | 83 | 3 | 1 | 174 | 86 | 236 | 1.60e-21 | 87.8 |
| MS.gene010738.t1 | MTR_2g036170 | 35.028 | 177 | 86 | 7 | 1 | 174 | 101 | 251 | 1.59e-16 | 74.7 |
| MS.gene010738.t1 | MTR_4g078170 | 28.571 | 175 | 78 | 4 | 1 | 174 | 80 | 208 | 2.45e-14 | 68.6 |
| MS.gene010738.t1 | MTR_5g030130 | 28.571 | 175 | 80 | 4 | 1 | 174 | 100 | 230 | 1.46e-13 | 66.6 |
| MS.gene010738.t1 | MTR_5g007230 | 40.580 | 69 | 40 | 1 | 1 | 68 | 86 | 154 | 7.93e-12 | 61.6 |
| MS.gene010738.t1 | MTR_7g102790 | 41.176 | 68 | 39 | 1 | 1 | 67 | 75 | 142 | 1.67e-11 | 60.5 |
| MS.gene010738.t1 | MTR_1g062160 | 28.000 | 175 | 79 | 6 | 1 | 174 | 73 | 201 | 5.38e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010738.t1 | AT3G60670 | 50.273 | 183 | 75 | 7 | 1 | 175 | 71 | 245 | 6.28e-43 | 143 |
| MS.gene010738.t1 | AT2G12646 | 35.079 | 191 | 102 | 5 | 1 | 174 | 70 | 255 | 2.42e-23 | 93.2 |
| MS.gene010738.t1 | AT2G12646 | 35.079 | 191 | 102 | 5 | 1 | 174 | 70 | 255 | 2.42e-23 | 93.2 |
| MS.gene010738.t1 | AT2G12646 | 35.079 | 191 | 102 | 5 | 1 | 174 | 67 | 252 | 2.44e-23 | 93.2 |
| MS.gene010738.t1 | AT1G31040 | 35.429 | 175 | 97 | 4 | 1 | 174 | 83 | 242 | 2.28e-21 | 87.8 |
| MS.gene010738.t1 | AT1G21000 | 30.682 | 176 | 110 | 6 | 1 | 174 | 78 | 243 | 2.24e-13 | 66.2 |
| MS.gene010738.t1 | AT1G21000 | 30.682 | 176 | 110 | 6 | 1 | 174 | 81 | 246 | 2.27e-13 | 66.2 |
| MS.gene010738.t1 | AT1G32700 | 41.791 | 67 | 38 | 1 | 1 | 66 | 38 | 104 | 1.97e-11 | 60.1 |
| MS.gene010738.t1 | AT1G32700 | 41.791 | 67 | 38 | 1 | 1 | 66 | 77 | 143 | 3.34e-11 | 60.1 |
| MS.gene010738.t1 | AT1G76590 | 30.114 | 176 | 108 | 8 | 1 | 174 | 83 | 245 | 4.77e-11 | 60.1 |
| MS.gene010738.t1 | AT2G27930 | 39.437 | 71 | 33 | 1 | 1 | 61 | 73 | 143 | 7.46e-11 | 58.5 |
| MS.gene010738.t1 | AT2G27930 | 39.437 | 71 | 33 | 1 | 1 | 61 | 73 | 143 | 7.46e-11 | 58.5 |
Find 50 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATTTGGTTCACTATC+TGG | 0.237092 | 7.4:+59600870 | MS.gene010738:CDS |
| GTAAGGGTCTTGAAGACTTC+TGG | 0.303743 | 7.4:-59600279 | None:intergenic |
| GAGGAGCATCTCTTTGGAAT+TGG | 0.338342 | 7.4:-59600233 | None:intergenic |
| TTGCTTGAGGAGCATCTCTT+TGG | 0.338603 | 7.4:-59600239 | None:intergenic |
| AACCGAACTTCATCCGGTTC+CGG | 0.371774 | 7.4:+59600990 | MS.gene010738:CDS |
| AGTCACTTGGTGAAAACATT+TGG | 0.391874 | 7.4:+59600858 | MS.gene010738:CDS |
| GAGAGAGAACAGAATTTGTA+AGG | 0.394736 | 7.4:-59600296 | None:intergenic |
| CACCGGAACCGGATGAAGTT+CGG | 0.397970 | 7.4:-59600992 | None:intergenic |
| TCTCGGAGAGTCTGATGAAT+AGG | 0.410323 | 7.4:+59601144 | MS.gene010738:CDS |
| GGCGATGTATCCACCGGAAC+CGG | 0.417575 | 7.4:-59601003 | None:intergenic |
| GGTTCAAACCGAACTTCATC+CGG | 0.422747 | 7.4:+59600984 | MS.gene010738:CDS |
| ATGCCACCATTATCTGATTC+TGG | 0.428827 | 7.4:+59600915 | MS.gene010738:CDS |
| GCCTGCATGCTCGCCGGTGT+CGG | 0.449198 | 7.4:+59601118 | MS.gene010738:CDS |
| CGGTGGTAGCGGTGCAAGCA+AGG | 0.471427 | 7.4:-59601043 | None:intergenic |
| GGATGAAGTTCGGTTTGAAC+CGG | 0.475769 | 7.4:-59600982 | None:intergenic |
| TCTAGTCCAGAATCAGATAA+TGG | 0.486066 | 7.4:-59600921 | None:intergenic |
| CTCGCCGGTGTCGGAAATCT+CGG | 0.497072 | 7.4:+59601127 | MS.gene010738:CDS |
| GATGGTTCGAGAACCGAGTC+AGG | 0.499983 | 7.4:-59600960 | None:intergenic |
| TCGGACAACCTCGGTGGTAG+CGG | 0.510984 | 7.4:-59601054 | None:intergenic |
| GTTGTCCGAAAGAAAAGAAC+CGG | 0.516356 | 7.4:+59601068 | MS.gene010738:CDS |
| ATTCTGTTCTCTCTCTTGCA+AGG | 0.523330 | 7.4:+59600304 | MS.gene010738:CDS |
| CAATATAACGGGGCCCTATG+TGG | 0.529393 | 7.4:-59601179 | None:intergenic |
| GACTCGGTTCTCGAACCATC+CGG | 0.529624 | 7.4:+59600963 | MS.gene010738:CDS |
| GTCTTGAAGACTTCTGGCAC+AGG | 0.531073 | 7.4:-59600273 | None:intergenic |
| GAGGAAAGGAACGCCACATA+GGG | 0.533397 | 7.4:+59601166 | MS.gene010738:CDS |
| TCCGACACCGGCGAGCATGC+AGG | 0.535651 | 7.4:-59601119 | None:intergenic |
| CTACAGTTCTACAGAACAAA+CGG | 0.540640 | 7.4:+59600182 | MS.gene010738:intron |
| GTGTCGGCCTGCATGCTCGC+CGG | 0.542423 | 7.4:+59601112 | MS.gene010738:CDS |
| CGGTCTAATGACACCTGACT+CGG | 0.553504 | 7.4:+59600947 | MS.gene010738:CDS |
| CGAACTTCATCCGGTTCCGG+TGG | 0.558699 | 7.4:+59600993 | MS.gene010738:CDS |
| GGTGGTAGCGGTGCAAGCAA+GGG | 0.563534 | 7.4:-59601042 | None:intergenic |
| CGGAGAGTCTGATGAATAGG+AGG | 0.571731 | 7.4:+59601147 | MS.gene010738:CDS |
| GGAGGAAAGGAACGCCACAT+AGG | 0.574415 | 7.4:+59601165 | MS.gene010738:CDS |
| AGTCTGATGAATAGGAGGAA+AGG | 0.580615 | 7.4:+59601152 | MS.gene010738:CDS |
| ATCAATGGCGATGTATCCAC+CGG | 0.592241 | 7.4:-59601009 | None:intergenic |
| ATGCAGGCCGACACGGTGGT+CGG | 0.595664 | 7.4:-59601103 | None:intergenic |
| GAAGTTCGGTTTGAACCGGA+TGG | 0.598179 | 7.4:-59600978 | None:intergenic |
| GGTTCTGCAGATATTGCTTG+AGG | 0.599864 | 7.4:-59600252 | None:intergenic |
| AGAATCAGATAATGGTGGCA+TGG | 0.607517 | 7.4:-59600913 | None:intergenic |
| GGTAGCGGTGCAAGCAAGGG+CGG | 0.608218 | 7.4:-59601039 | None:intergenic |
| TCTGATTCTGGACTAGATGA+CGG | 0.614207 | 7.4:+59600927 | MS.gene010738:CDS |
| AGAGAGAACAGAATTTGTAA+GGG | 0.616060 | 7.4:-59600295 | None:intergenic |
| GGCGAGCATGCAGGCCGACA+CGG | 0.620248 | 7.4:-59601110 | None:intergenic |
| CTCTCCGAGATTTCCGACAC+CGG | 0.623268 | 7.4:-59601131 | None:intergenic |
| TCACTTTCCGACCACCGTGT+CGG | 0.647068 | 7.4:+59601096 | MS.gene010738:CDS |
| AGTCCAGAATCAGATAATGG+TGG | 0.683117 | 7.4:-59600918 | None:intergenic |
| TTTCTTTCGGACAACCTCGG+TGG | 0.687052 | 7.4:-59601060 | None:intergenic |
| TGCTTGCACCGCTACCACCG+AGG | 0.713943 | 7.4:+59601046 | MS.gene010738:CDS |
| GAGCATGCAGGCCGACACGG+TGG | 0.741450 | 7.4:-59601107 | None:intergenic |
| TGGTCGGAAAGTGATGACAC+CGG | 0.742801 | 7.4:-59601087 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTAAACTATGTATAT+TGG | + | chr7.4:59600029-59600048 | MS.gene010738:intron | 10.0% |
| !!! | TTAATTTTTTACAAGAATTT+AGG | + | chr7.4:59600089-59600108 | MS.gene010738:intron | 10.0% |
| !!! | AAAAAATATGATGATGAAAA+TGG | - | chr7.4:59600644-59600663 | None:intergenic | 15.0% |
| !!! | TTGGAATTTTTAAGTATAAT+TGG | + | chr7.4:59600423-59600442 | MS.gene010738:intron | 15.0% |
| !! | ATATTCAATTCGTGTAAAAT+TGG | - | chr7.4:59600538-59600557 | None:intergenic | 20.0% |
| !!! | ATGTTGAATTTAGTTTTAGT+TGG | + | chr7.4:59600586-59600605 | MS.gene010738:intron | 20.0% |
| !!! | TAATTGTTTGTCTGATTTTA+TGG | - | chr7.4:59600746-59600765 | None:intergenic | 20.0% |
| !!! | TACAATGATTTTATCATGTT+TGG | + | chr7.4:59600404-59600423 | MS.gene010738:intron | 20.0% |
| ! | AGATTAAATATGAATGTGAG+AGG | - | chr7.4:59600339-59600358 | None:intergenic | 25.0% |
| !! | CTATTCAAGTAAGTGATTTT+TGG | + | chr7.4:59599976-59599995 | MS.gene010738:intron | 25.0% |
| !! | TACCACAATTAATTTTGTGT+TGG | + | chr7.4:59600145-59600164 | MS.gene010738:intron | 25.0% |
| !!! | TTCAATTTTTAGTGTGTGTT+TGG | + | chr7.4:59600363-59600382 | MS.gene010738:intron | 25.0% |
| AGAGAGAACAGAATTTGTAA+GGG | - | chr7.4:59600298-59600317 | None:intergenic | 30.0% | |
| CACCAACACAAAATTAATTG+TGG | - | chr7.4:59600150-59600169 | None:intergenic | 30.0% | |
| ! | AAAACATTTGGTTCACTATC+TGG | + | chr7.4:59600870-59600889 | MS.gene010738:CDS | 30.0% |
| CTACAGTTCTACAGAACAAA+CGG | + | chr7.4:59600182-59600201 | MS.gene010738:intron | 35.0% | |
| GAGAGAGAACAGAATTTGTA+AGG | - | chr7.4:59600299-59600318 | None:intergenic | 35.0% | |
| TCTAGTCCAGAATCAGATAA+TGG | - | chr7.4:59600924-59600943 | None:intergenic | 35.0% | |
| ! | AGTCACTTGGTGAAAACATT+TGG | + | chr7.4:59600858-59600877 | MS.gene010738:CDS | 35.0% |
| ! | TTTATGGTTGTGTTTTCCAC+AGG | + | chr7.4:59600833-59600852 | MS.gene010738:intron | 35.0% |
| AGTCTGATGAATAGGAGGAA+AGG | + | chr7.4:59601152-59601171 | MS.gene010738:CDS | 40.0% | |
| ATGCCACCATTATCTGATTC+TGG | + | chr7.4:59600915-59600934 | MS.gene010738:CDS | 40.0% | |
| ATTCTGTTCTCTCTCTTGCA+AGG | + | chr7.4:59600304-59600323 | MS.gene010738:CDS | 40.0% | |
| GTTGTCCGAAAGAAAAGAAC+CGG | + | chr7.4:59601068-59601087 | MS.gene010738:CDS | 40.0% | |
| ! | AGAATCAGATAATGGTGGCA+TGG | - | chr7.4:59600916-59600935 | None:intergenic | 40.0% |
| ! | AGTCCAGAATCAGATAATGG+TGG | - | chr7.4:59600921-59600940 | None:intergenic | 40.0% |
| ! | TCTGATTCTGGACTAGATGA+CGG | + | chr7.4:59600927-59600946 | MS.gene010738:CDS | 40.0% |
| ! | TCTTTTCTTTCGGACAACCT+CGG | - | chr7.4:59601066-59601085 | None:intergenic | 40.0% |
| !!! | TGACACCGGTTCTTTTCTTT+CGG | - | chr7.4:59601076-59601095 | None:intergenic | 40.0% |
| ATCAATGGCGATGTATCCAC+CGG | - | chr7.4:59601012-59601031 | None:intergenic | 45.0% | |
| GGTTCAAACCGAACTTCATC+CGG | + | chr7.4:59600984-59601003 | MS.gene010738:CDS | 45.0% | |
| GGTTCTGCAGATATTGCTTG+AGG | - | chr7.4:59600255-59600274 | None:intergenic | 45.0% | |
| GTAAGGGTCTTGAAGACTTC+TGG | - | chr7.4:59600282-59600301 | None:intergenic | 45.0% | |
| TCTCGGAGAGTCTGATGAAT+AGG | + | chr7.4:59601144-59601163 | MS.gene010738:CDS | 45.0% | |
| ! | AAGGGCGGTTTTGCAATCAA+TGG | - | chr7.4:59601027-59601046 | None:intergenic | 45.0% |
| ! | GGATGAAGTTCGGTTTGAAC+CGG | - | chr7.4:59600985-59601004 | None:intergenic | 45.0% |
| ! | TTTTCCACAGGTGAGTCACT+TGG | + | chr7.4:59600845-59600864 | MS.gene010738:intron | 45.0% |
| !! | GAGGAGCATCTCTTTGGAAT+TGG | - | chr7.4:59600236-59600255 | None:intergenic | 45.0% |
| !! | TTGCTTGAGGAGCATCTCTT+TGG | - | chr7.4:59600242-59600261 | None:intergenic | 45.0% |
| !!! | ATTTTTTTTTTGTTATTTTA+TGG | + | chr7.4:59600817-59600836 | MS.gene010738:intron | 5.0% |
| AACCGAACTTCATCCGGTTC+CGG | + | chr7.4:59600990-59601009 | MS.gene010738:CDS | 50.0% | |
| CGGAGAGTCTGATGAATAGG+AGG | + | chr7.4:59601147-59601166 | MS.gene010738:CDS | 50.0% | |
| CGGTCTAATGACACCTGACT+CGG | + | chr7.4:59600947-59600966 | MS.gene010738:CDS | 50.0% | |
| GAGGAAAGGAACGCCACATA+GGG | + | chr7.4:59601166-59601185 | MS.gene010738:CDS | 50.0% | |
| GTCTTGAAGACTTCTGGCAC+AGG | - | chr7.4:59600276-59600295 | None:intergenic | 50.0% | |
| TGGTCGGAAAGTGATGACAC+CGG | - | chr7.4:59601090-59601109 | None:intergenic | 50.0% | |
| TTCACCAAGTGACTCACCTG+TGG | - | chr7.4:59600852-59600871 | None:intergenic | 50.0% | |
| TTTCTTTCGGACAACCTCGG+TGG | - | chr7.4:59601063-59601082 | None:intergenic | 50.0% | |
| ! | CAATATAACGGGGCCCTATG+TGG | - | chr7.4:59601182-59601201 | None:intergenic | 50.0% |
| ! | GAAGTTCGGTTTGAACCGGA+TGG | - | chr7.4:59600981-59601000 | None:intergenic | 50.0% |
| CACCGGAACCGGATGAAGTT+CGG | - | chr7.4:59600995-59601014 | None:intergenic | 55.0% | |
| CTCTCCGAGATTTCCGACAC+CGG | - | chr7.4:59601134-59601153 | None:intergenic | 55.0% | |
| GATGGTTCGAGAACCGAGTC+AGG | - | chr7.4:59600963-59600982 | None:intergenic | 55.0% | |
| GGAGGAAAGGAACGCCACAT+AGG | + | chr7.4:59601165-59601184 | MS.gene010738:CDS | 55.0% | |
| TCACTTTCCGACCACCGTGT+CGG | + | chr7.4:59601096-59601115 | MS.gene010738:CDS | 55.0% | |
| ! | GACTCGGTTCTCGAACCATC+CGG | + | chr7.4:59600963-59600982 | MS.gene010738:CDS | 55.0% |
| CGAACTTCATCCGGTTCCGG+TGG | + | chr7.4:59600993-59601012 | MS.gene010738:CDS | 60.0% | |
| GGCGATGTATCCACCGGAAC+CGG | - | chr7.4:59601006-59601025 | None:intergenic | 60.0% | |
| TCGGACAACCTCGGTGGTAG+CGG | - | chr7.4:59601057-59601076 | None:intergenic | 60.0% | |
| ! | CTCGCCGGTGTCGGAAATCT+CGG | + | chr7.4:59601127-59601146 | MS.gene010738:CDS | 60.0% |
| !! | GGTGGTAGCGGTGCAAGCAA+GGG | - | chr7.4:59601045-59601064 | None:intergenic | 60.0% |
| ATGCAGGCCGACACGGTGGT+CGG | - | chr7.4:59601106-59601125 | None:intergenic | 65.0% | |
| TGCTTGCACCGCTACCACCG+AGG | + | chr7.4:59601046-59601065 | MS.gene010738:CDS | 65.0% | |
| ! | GGTAGCGGTGCAAGCAAGGG+CGG | - | chr7.4:59601042-59601061 | None:intergenic | 65.0% |
| !! | CGGTGGTAGCGGTGCAAGCA+AGG | - | chr7.4:59601046-59601065 | None:intergenic | 65.0% |
| GAGCATGCAGGCCGACACGG+TGG | - | chr7.4:59601110-59601129 | None:intergenic | 70.0% | |
| GCCTGCATGCTCGCCGGTGT+CGG | + | chr7.4:59601118-59601137 | MS.gene010738:CDS | 70.0% | |
| GGCGAGCATGCAGGCCGACA+CGG | - | chr7.4:59601113-59601132 | None:intergenic | 70.0% | |
| TCCGACACCGGCGAGCATGC+AGG | - | chr7.4:59601122-59601141 | None:intergenic | 70.0% | |
| ! | GTGTCGGCCTGCATGCTCGC+CGG | + | chr7.4:59601112-59601131 | MS.gene010738:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 59599942 | 59601202 | 59599942 | ID=MS.gene010738 |
| chr7.4 | mRNA | 59599942 | 59601202 | 59599942 | ID=MS.gene010738.t1;Parent=MS.gene010738 |
| chr7.4 | exon | 59599942 | 59599983 | 59599942 | ID=MS.gene010738.t1.exon1;Parent=MS.gene010738.t1 |
| chr7.4 | CDS | 59599942 | 59599983 | 59599942 | ID=cds.MS.gene010738.t1;Parent=MS.gene010738.t1 |
| chr7.4 | exon | 59600188 | 59600325 | 59600188 | ID=MS.gene010738.t1.exon2;Parent=MS.gene010738.t1 |
| chr7.4 | CDS | 59600188 | 59600325 | 59600188 | ID=cds.MS.gene010738.t1;Parent=MS.gene010738.t1 |
| chr7.4 | exon | 59600855 | 59601202 | 59600855 | ID=MS.gene010738.t1.exon3;Parent=MS.gene010738.t1 |
| chr7.4 | CDS | 59600855 | 59601202 | 59600855 | ID=cds.MS.gene010738.t1;Parent=MS.gene010738.t1 |
| Gene Sequence |
| Protein sequence |
