AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010855

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010855.t1 MTR_7g445710 90.047 211 20 1 1 210 333 543 5.47e-136 392
MS.gene010855.t1 MTR_7g445530 88.950 181 19 1 1 180 333 513 7.79e-113 332
MS.gene010855.t1 MTR_7g450730 74.762 210 28 1 1 210 321 505 2.18e-106 315
MS.gene010855.t1 MTR_5g013420 48.319 238 80 4 2 210 866 1089 5.38e-64 214
MS.gene010855.t1 MTR_5g013420 47.899 238 81 4 2 210 866 1089 8.90e-63 210
MS.gene010855.t1 MTR_7g445600 61.538 169 14 1 12 180 239 356 2.38e-62 197
MS.gene010855.t1 MTR_4g010830 46.218 238 85 4 2 210 757 980 5.08e-62 207
MS.gene010855.t1 MTR_4g010550 42.678 239 76 6 2 210 257 464 5.37e-49 166
MS.gene010855.t1 MTR_7g084090 39.000 200 110 3 13 210 568 757 4.12e-41 148
MS.gene010855.t1 MTR_6g061270 37.143 210 117 4 3 210 498 694 1.47e-38 140
MS.gene010855.t1 MTR_7g052480 36.019 211 120 6 2 210 359 556 3.91e-38 138
MS.gene010855.t1 MTR_1g048950 35.500 200 81 3 13 210 380 533 1.64e-34 129
MS.gene010855.t1 MTR_3g011440 36.041 197 105 5 15 205 478 659 2.46e-33 126
MS.gene010855.t1 MTR_8g078490 38.384 198 86 6 15 210 1090 1253 1.15e-32 124
MS.gene010855.t1 MTR_6g061200 33.654 208 98 4 3 210 498 665 2.35e-31 120
MS.gene010855.t1 MTR_4g132610 35.500 200 89 5 15 210 884 1047 2.99e-31 120
MS.gene010855.t1 MTR_8g042750 35.354 198 104 6 15 205 503 683 6.47e-31 119
MS.gene010855.t1 MTR_5g018850 33.831 201 91 4 15 210 1344 1507 1.77e-29 115
MS.gene010855.t1 MTR_7g088370 35.233 193 95 6 15 205 536 700 9.50e-29 113
MS.gene010855.t1 MTR_8g071030 31.553 206 98 3 3 179 544 735 1.56e-28 112
MS.gene010855.t1 MTR_8g078505 34.197 193 88 5 15 205 378 533 3.00e-27 108
MS.gene010855.t1 MTR_8g070070 30.476 210 97 4 3 180 564 756 4.13e-26 105
MS.gene010855.t1 MTR_8g070075 34.545 165 94 3 15 179 598 748 1.07e-25 104
MS.gene010855.t1 MTR_1g035420 32.335 167 97 3 15 180 494 645 3.16e-25 102
MS.gene010855.t1 MTR_2g054460 30.939 181 103 5 3 180 302 463 1.68e-24 100
MS.gene010855.t1 MTR_6g044690 31.548 168 96 4 15 179 411 562 1.98e-24 100
MS.gene010855.t1 MTR_6g043960 30.357 168 98 4 15 179 329 480 1.19e-23 98.2
MS.gene010855.t1 MTR_0376s0030 30.556 180 103 3 3 179 400 560 1.26e-23 98.2
MS.gene010855.t1 MTR_1g035400 29.885 174 106 3 15 187 413 571 5.68e-20 87.8
MS.gene010855.t1 MTR_1g080340 32.057 209 109 8 2 208 407 584 8.72e-20 87.4
MS.gene010855.t1 MTR_1g069570 30.952 210 103 8 2 205 568 741 1.09e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010855.t1 AT2G22740 45.378 238 86 4 3 210 567 790 2.29e-61 204
MS.gene010855.t1 AT2G22740 45.378 238 86 4 3 210 567 790 2.29e-61 204
MS.gene010855.t1 AT2G35160 42.373 236 68 3 3 210 563 758 5.68e-58 195
MS.gene010855.t1 AT2G35160 42.373 236 68 3 3 210 599 794 8.76e-58 194
MS.gene010855.t1 AT2G35160 42.373 236 68 3 3 210 599 794 8.76e-58 194
MS.gene010855.t1 AT2G35160 42.373 236 68 3 3 210 599 794 8.76e-58 194
MS.gene010855.t1 AT2G35160 42.373 236 68 3 3 210 599 794 8.76e-58 194
MS.gene010855.t1 AT5G13960 37.019 208 119 4 3 210 295 490 6.10e-39 140
MS.gene010855.t1 AT5G13960 37.019 208 119 4 3 210 429 624 1.97e-38 140
MS.gene010855.t1 AT1G73100 34.021 194 110 5 15 205 486 664 3.41e-28 111
MS.gene010855.t1 AT5G04940 34.171 199 103 8 15 205 487 665 8.63e-28 110
MS.gene010855.t1 AT5G04940 34.171 199 103 8 15 205 487 665 8.63e-28 110
MS.gene010855.t1 AT2G23740 31.633 196 95 4 15 205 1209 1370 1.00e-24 101
MS.gene010855.t1 AT2G23740 31.633 196 95 4 15 205 1216 1377 1.03e-24 101
MS.gene010855.t1 AT2G23740 31.633 196 95 4 15 205 1216 1377 1.03e-24 101
MS.gene010855.t1 AT2G23740 31.633 196 95 4 15 205 1216 1377 1.03e-24 101
MS.gene010855.t1 AT4G13460 34.545 165 95 2 15 179 485 636 1.31e-24 101
MS.gene010855.t1 AT4G13460 34.545 165 95 2 15 179 485 636 1.31e-24 101
MS.gene010855.t1 AT2G24740 30.736 231 113 7 3 205 539 750 1.69e-23 98.2
MS.gene010855.t1 AT1G17770 31.282 195 113 4 15 205 511 688 1.30e-22 95.5
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 257 433 1.73e-21 92.0
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 257 433 1.82e-21 91.7
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 241 417 1.90e-21 91.7
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 284 460 2.47e-21 91.7
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 284 460 2.54e-21 91.7
MS.gene010855.t1 AT3G04380 32.536 209 107 7 2 208 268 444 2.88e-21 91.3
MS.gene010855.t1 AT2G33290 30.168 179 104 4 3 179 478 637 2.36e-20 89.0
MS.gene010855.t1 AT2G33290 30.168 179 104 4 3 179 478 637 2.36e-20 89.0
MS.gene010855.t1 AT2G33290 30.168 179 104 4 3 179 478 637 2.36e-20 89.0
MS.gene010855.t1 AT2G33290 30.168 179 104 4 3 179 478 637 2.36e-20 89.0
MS.gene010855.t1 AT1G04050 29.612 206 108 7 2 205 548 718 8.63e-18 81.6
MS.gene010855.t1 AT1G04050 29.612 206 108 7 2 205 548 718 8.63e-18 81.6
MS.gene010855.t1 AT1G04050 29.612 206 108 7 2 205 444 614 9.12e-18 81.6
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 443 615 1.66e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 535 707 1.67e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 535 707 1.67e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 463 635 1.69e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 463 635 1.69e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 463 635 1.69e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 463 635 1.69e-16 77.8
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 555 727 1.76e-16 77.8
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 555 727 1.76e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 512 684 1.87e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 512 684 1.87e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 512 684 1.87e-16 77.8
MS.gene010855.t1 AT5G43990 28.230 209 112 6 2 208 512 684 1.87e-16 77.8
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 532 704 2.03e-16 77.4
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 532 704 2.03e-16 77.4
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 532 704 2.03e-16 77.4
MS.gene010855.t1 AT5G43990 28.708 209 111 6 2 208 532 704 2.03e-16 77.4
MS.gene010855.t1 AT2G23740 31.783 129 53 3 15 138 1216 1314 3.80e-13 68.2
MS.gene010855.t1 AT2G23740 31.783 129 53 3 15 138 1216 1314 3.80e-13 68.2
MS.gene010855.t1 AT1G76710 32.065 184 74 9 24 205 90 224 7.68e-13 67.0
MS.gene010855.t1 AT1G76710 32.065 184 74 9 24 205 90 224 8.08e-13 67.0
MS.gene010855.t1 AT1G76710 32.065 184 74 9 24 205 90 224 8.79e-13 67.0
MS.gene010855.t1 AT1G76710 32.065 184 74 9 24 205 90 224 8.79e-13 67.0
MS.gene010855.t1 AT3G61740 27.717 184 87 6 23 205 839 977 8.85e-11 61.2

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACCACTTGAAGCTTCTA+AGG 0.221245 7.2:+54326876 None:intergenic
AACATTTCGAGCAAACAAAT+TGG 0.261209 7.2:+54326759 None:intergenic
ACCATTGATGCTGCTAAGTA+TGG 0.290913 7.2:-54326817 MS.gene010855:CDS
TCAGAGAATGCAATTCCTTT+TGG 0.305406 7.2:-54327069 MS.gene010855:CDS
TTCATATATGGTTGATAGTA+AGG 0.403159 7.2:-54326917 MS.gene010855:CDS
ATTATATTCACAAGGTCTAT+AGG 0.407539 7.2:-54326954 MS.gene010855:CDS
ACAAATTGGGAGAACAACTA+TGG 0.427505 7.2:+54326773 None:intergenic
AATCAAGTGATTGATTCAAA+TGG 0.439888 7.2:-54326634 MS.gene010855:CDS
ACATTTCGAGCAAACAAATT+GGG 0.449844 7.2:+54326760 None:intergenic
GTGGGAAGAGGATTCATATA+TGG 0.450043 7.2:-54326929 MS.gene010855:CDS
GGTCTATAGGTCGTGGGAAG+AGG 0.454401 7.2:-54326941 MS.gene010855:CDS
TCAAGACTAAAGCGAAAGGA+TGG 0.483022 7.2:-54327101 MS.gene010855:CDS
ACCATACTTAGCAGCATCAA+TGG 0.496537 7.2:+54326816 None:intergenic
AAAGTCAGCTCATTCATGCT+TGG 0.503473 7.2:+54326676 None:intergenic
TTCACAAGGTCTATAGGTCG+TGG 0.504274 7.2:-54326948 MS.gene010855:CDS
AAGACGAAAGCATGTTATTG+TGG 0.528019 7.2:-54326604 MS.gene010855:CDS
TCACAAATAAAACTTCCAAA+AGG 0.534102 7.2:+54327054 None:intergenic
CAAGACTAAAGCGAAAGGAT+GGG 0.536026 7.2:-54327100 MS.gene010855:CDS
TTACCACTTGAAGCTTCTAA+GGG 0.542917 7.2:+54326877 None:intergenic
CCCTCCACTCTTAAGAGCAC+AGG 0.546581 7.2:+54327173 None:intergenic
GTTGATGATCGATGTGATAG+TGG 0.551003 7.2:-54326847 MS.gene010855:CDS
AGAAGCTTCAAGTGGTAAAG+AGG 0.552219 7.2:-54326872 MS.gene010855:CDS
AGGTGAGATTATTAAAGATG+AGG 0.556261 7.2:-54327025 MS.gene010855:CDS
CTAAGTATGGTAACGTGGCA+AGG 0.558339 7.2:-54326804 MS.gene010855:CDS
TCCTGTGCTCTTAAGAGTGG+AGG 0.560747 7.2:-54327174 MS.gene010855:CDS
AAGACTAAAGCGAAAGGATG+GGG 0.572218 7.2:-54327099 MS.gene010855:CDS
ATGATCGATGTGATAGTGGT+AGG 0.595335 7.2:-54326843 MS.gene010855:CDS
TGTTCCTGTGCTCTTAAGAG+TGG 0.599372 7.2:-54327177 MS.gene010855:CDS
CCTGTGCTCTTAAGAGTGGA+GGG 0.600581 7.2:-54327173 MS.gene010855:CDS
AATCTCATCAATACAGTCGA+CGG 0.610597 7.2:+54326566 None:intergenic
TCACAAGGTCTATAGGTCGT+GGG 0.621148 7.2:-54326947 MS.gene010855:CDS
TATTAGCAATTATATTCACA+AGG 0.626162 7.2:-54326962 MS.gene010855:CDS
TTTGCGATGATGATGATCTG+AGG 0.630579 7.2:-54326735 MS.gene010855:CDS
CAACCCTTAGAAGCTTCAAG+TGG 0.647600 7.2:-54326880 MS.gene010855:CDS
ACTATCACATCGATCATCAA+CGG 0.657622 7.2:+54326849 None:intergenic
GCTGCATATAGCATGATGTG+TGG 0.717627 7.2:+54326709 None:intergenic
TGCTGCTAAGTATGGTAACG+TGG 0.728265 7.2:-54326809 MS.gene010855:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAGCAATTATATTCACA+AGG - chr7.2:54326794-54326813 MS.gene010855:CDS 20.0%
!!! AGTTTTATTTGTGAGTATTT+AGG - chr7.2:54326711-54326730 MS.gene010855:CDS 20.0%
! ATTATATTCACAAGGTCTAT+AGG - chr7.2:54326802-54326821 MS.gene010855:CDS 25.0%
! TCACAAATAAAACTTCCAAA+AGG + chr7.2:54326705-54326724 None:intergenic 25.0%
!! AATCAAGTGATTGATTCAAA+TGG - chr7.2:54327122-54327141 MS.gene010855:CDS 25.0%
!! ATCTGATTTTTCTTTACTCT+AGG + chr7.2:54326615-54326634 None:intergenic 25.0%
!!! TTCATATATGGTTGATAGTA+AGG - chr7.2:54326839-54326858 MS.gene010855:CDS 25.0%
AACATTTCGAGCAAACAAAT+TGG + chr7.2:54327000-54327019 None:intergenic 30.0%
ACATTTCGAGCAAACAAATT+GGG + chr7.2:54326999-54327018 None:intergenic 30.0%
AGGTGAGATTATTAAAGATG+AGG - chr7.2:54326731-54326750 MS.gene010855:CDS 30.0%
!! ATTTTCAAGACTAAAGCGAA+AGG - chr7.2:54326651-54326670 MS.gene010855:CDS 30.0%
ACAAATTGGGAGAACAACTA+TGG + chr7.2:54326986-54327005 None:intergenic 35.0%
ACTATCACATCGATCATCAA+CGG + chr7.2:54326910-54326929 None:intergenic 35.0%
TTACCACTTGAAGCTTCTAA+GGG + chr7.2:54326882-54326901 None:intergenic 35.0%
TTTACCACTTGAAGCTTCTA+AGG + chr7.2:54326883-54326902 None:intergenic 35.0%
! AAGACGAAAGCATGTTATTG+TGG - chr7.2:54327152-54327171 MS.gene010855:CDS 35.0%
! TCAGAGAATGCAATTCCTTT+TGG - chr7.2:54326687-54326706 MS.gene010855:CDS 35.0%
AAAGTCAGCTCATTCATGCT+TGG + chr7.2:54327083-54327102 None:intergenic 40.0%
AAGACTAAAGCGAAAGGATG+GGG - chr7.2:54326657-54326676 MS.gene010855:CDS 40.0%
ACCATACTTAGCAGCATCAA+TGG + chr7.2:54326943-54326962 None:intergenic 40.0%
ACCATTGATGCTGCTAAGTA+TGG - chr7.2:54326939-54326958 MS.gene010855:CDS 40.0%
AGAAGCTTCAAGTGGTAAAG+AGG - chr7.2:54326884-54326903 MS.gene010855:CDS 40.0%
CAAGACTAAAGCGAAAGGAT+GGG - chr7.2:54326656-54326675 MS.gene010855:CDS 40.0%
GTGGGAAGAGGATTCATATA+TGG - chr7.2:54326827-54326846 MS.gene010855:CDS 40.0%
TCAAGACTAAAGCGAAAGGA+TGG - chr7.2:54326655-54326674 MS.gene010855:CDS 40.0%
! TTTGCGATGATGATGATCTG+AGG - chr7.2:54327021-54327040 MS.gene010855:CDS 40.0%
!! ATGATCGATGTGATAGTGGT+AGG - chr7.2:54326913-54326932 MS.gene010855:CDS 40.0%
!! GTTGATGATCGATGTGATAG+TGG - chr7.2:54326909-54326928 MS.gene010855:CDS 40.0%
CAACCCTTAGAAGCTTCAAG+TGG - chr7.2:54326876-54326895 MS.gene010855:CDS 45.0%
GCTGCATATAGCATGATGTG+TGG + chr7.2:54327050-54327069 None:intergenic 45.0%
TCACAAGGTCTATAGGTCGT+GGG - chr7.2:54326809-54326828 MS.gene010855:CDS 45.0%
TGTTCCTGTGCTCTTAAGAG+TGG - chr7.2:54326579-54326598 MS.gene010855:CDS 45.0%
TTCACAAGGTCTATAGGTCG+TGG - chr7.2:54326808-54326827 MS.gene010855:CDS 45.0%
! CTAAGTATGGTAACGTGGCA+AGG - chr7.2:54326952-54326971 MS.gene010855:CDS 45.0%
! TGCTGCTAAGTATGGTAACG+TGG - chr7.2:54326947-54326966 MS.gene010855:CDS 45.0%
CCTGTGCTCTTAAGAGTGGA+GGG - chr7.2:54326583-54326602 MS.gene010855:CDS 50.0%
TCCTGTGCTCTTAAGAGTGG+AGG - chr7.2:54326582-54326601 MS.gene010855:CDS 50.0%
GGTCTATAGGTCGTGGGAAG+AGG - chr7.2:54326815-54326834 MS.gene010855:CDS 55.0%
! CCCTCCACTCTTAAGAGCAC+AGG + chr7.2:54326586-54326605 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 54326573 54327205 54326573 ID=MS.gene010855
chr7.2 mRNA 54326573 54327205 54326573 ID=MS.gene010855.t1;Parent=MS.gene010855
chr7.2 exon 54326573 54327205 54326573 ID=MS.gene010855.t1.exon1;Parent=MS.gene010855.t1
chr7.2 CDS 54326573 54327205 54326573 ID=cds.MS.gene010855.t1;Parent=MS.gene010855.t1
Gene Sequence

>MS.gene010855

ATGAAATGTTCCTGTGCTCTTAAGAGTGGAGGGAGAATTCCTAGAGTAAAGAAAAATCAGATTAAATTTAAGCTTCAAATTTTCAAGACTAAAGCGAAAGGATGGGGTGTGAGATCAGAGAATGCAATTCCTTTTGGAAGTTTTATTTGTGAGTATTTAGGTGAGATTATTAAAGATGAGGAAGCTTTGAAAATAGTTGATAATGATGAGTATTTATTTAATATTAGCAATTATATTCACAAGGTCTATAGGTCGTGGGAAGAGGATTCATATATGGTTGATAGTAAGGAGATGAGTTCATATCAACCCTTAGAAGCTTCAAGTGGTAAAGAGGCCGTTGATGATCGATGTGATAGTGGTAGGTTTACCATTGATGCTGCTAAGTATGGTAACGTGGCAAGGTTTATCAACCATAGTTGTTCTCCCAATTTGTTTGCTCGAAATGTTCTTTGCGATGATGATGATCTGAGGATTCCACACATCATGCTATATGCAGCTGAGAACATTCCAAGCATGAATGAGCTGACTTTGAATTACAATTACAAGATCAATCAAGTGATTGATTCAAATGGAAATTTAAAGACGAAAGCATGTTATTGTGGAGCTTCTGAATGCATCCGTCGACTGTATTGA
Protein sequence

>MS.gene010855.t1

MKCSCALKSGGRIPRVKKNQIKFKLQIFKTKAKGWGVRSENAIPFGSFICEYLGEIIKDEEALKIVDNDEYLFNISNYIHKVYRSWEEDSYMVDSKEMSSYQPLEASSGKEAVDDRCDSGRFTIDAAKYGNVARFINHSCSPNLFARNVLCDDDDLRIPHIMLYAAENIPSMNELTLNYNYKINQVIDSNGNLKTKACYCGASECIRRLY