AlfalfaGEDB | MS.gene010899

M. sativa cultivar XinJiangDaYe / MS.gene010899

KEGG: K20791
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010899.t1	RHN67051.1	81.9	155	27	1	1	154	1	155	4.20E-63	250.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010899.t1	G7J1Z7	81.9	155	27	1	1	154	1	155	3.1e-63	250.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene010899.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010899.t1	MTR_3g050860	81.935	155	27	1	1	154	1	155	1.99e-87	259
MS.gene010899.t1	MTR_0439s0020	80.420	143	26	1	1	141	1	143	1.12e-79	232

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCGTCAGTGTTGGTCTTC+GGG	0.301799	7.2:+53050893	None:intergenic
GGTTTCATCGTCTTTGATAT+TGG	0.310497	7.2:-53051184	MS.gene010899:CDS
AACCTTGTCCAAGTCCATTT+CGG	0.354048	7.2:+53051066	None:intergenic
AATGCGTCAGTGTTGGTCTT+CGG	0.376239	7.2:+53050892	None:intergenic
ACTTGTGACCATGGCCGAAA+TGG	0.380600	7.2:-53051080	MS.gene010899:CDS
CATCCACAAGAATCTTAAAT+AGG	0.405177	7.2:+53051293	None:intergenic
AACCCTATCTTTCGGAGAAA+AGG	0.415290	7.2:-53051115	MS.gene010899:CDS
GTGAAGAGCAATAATATTGA+TGG	0.429245	7.2:-53050835	MS.gene010899:CDS
AGAATCTTAAATAGGTCAAC+AGG	0.430459	7.2:+53051301	None:intergenic
TGACGTGGATGCTAAAAGTT+TGG	0.430756	7.2:-53051260	MS.gene010899:CDS
TCTTCGTTAACCCTATCTTT+CGG	0.431368	7.2:-53051123	MS.gene010899:CDS
GTCCAAGTCCATTTCGGCCA+TGG	0.446272	7.2:+53051072	None:intergenic
GACCATGGCCGAAATGGACT+TGG	0.499061	7.2:-53051074	MS.gene010899:CDS
GATCTGAAGCATACTGGTTA+TGG	0.504056	7.2:-53051160	MS.gene010899:CDS
TGACCTATTTAAGATTCTTG+TGG	0.505182	7.2:-53051296	MS.gene010899:CDS
GAGCAATAATATTGATGGGA+AGG	0.524325	7.2:-53050830	MS.gene010899:CDS
ATTGGTGATCTGAAGCATAC+TGG	0.528676	7.2:-53051166	MS.gene010899:CDS
GTCGCCGAGGATAAACTCCT+AGG	0.541691	7.2:-53051217	MS.gene010899:CDS
GGCCGAAATGGACTTGGACA+AGG	0.545609	7.2:-53051068	MS.gene010899:intron
TGAAGAGCAATAATATTGAT+GGG	0.562579	7.2:-53050834	MS.gene010899:CDS
GCATCTCTTACGACCACATG+TGG	0.566352	7.2:+53051328	None:intergenic
AAACTCCTAGGGTTTATCCT+CGG	0.580594	7.2:-53051205	MS.gene010899:CDS
ATCATGACGAAGAAGCTCGA+GGG	0.582010	7.2:-53050793	MS.gene010899:CDS
CGCTACTCGACTTGTGACCA+TGG	0.583316	7.2:-53051089	MS.gene010899:CDS
TAAGAGTAATGCGTCAGTGT+TGG	0.594559	7.2:+53050885	None:intergenic
AAAGATAGGGTTAACGAAGA+GGG	0.602204	7.2:+53051126	None:intergenic
TCGCCGAGGATAAACTCCTA+GGG	0.604901	7.2:-53051216	MS.gene010899:CDS
AAACCCTAGGAGTTTATCCT+CGG	0.607628	7.2:+53051213	None:intergenic
GAAAGATAGGGTTAACGAAG+AGG	0.620492	7.2:+53051125	None:intergenic
GTCAGTGTTGGTCTTCGGGA+CGG	0.627712	7.2:+53050897	None:intergenic
ATCTCTTACGACCACATGTG+GGG	0.629012	7.2:+53051330	None:intergenic
TGAAACCGAGGATAAACCCT+AGG	0.631949	7.2:+53051200	None:intergenic
CATCTCTTACGACCACATGT+GGG	0.632600	7.2:+53051329	None:intergenic
GATGCTAAAAGTTTGGCGAA+TGG	0.635951	7.2:-53051253	MS.gene010899:CDS
CATCATGACGAAGAAGCTCG+AGG	0.646448	7.2:-53050794	MS.gene010899:CDS
TCATGACGAAGAAGCTCGAG+GGG	0.655307	7.2:-53050792	MS.gene010899:CDS
AAAGATGGAGTCCCCACATG+TGG	0.657747	7.2:-53051341	None:intergenic
TATCAAAGACGATGAAACCG+AGG	0.669702	7.2:+53051188	None:intergenic
TAAGATTCTTCAAGTCGCCG+AGG	0.715340	7.2:-53051230	MS.gene010899:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTGTCTTCTTTTAATTTAA+TGG	-	chr7.2:53051114-53051133	MS.gene010899:CDS	20.0%
!	AAAATTATGACAGAGTCAAT+AGG	+	chr7.2:53051081-53051100	None:intergenic	25.0%
!	ATTCAAAAAGATGAAGTATG+AGG	-	chr7.2:53051245-53051264	MS.gene010899:CDS	25.0%
!	TGAAGAGCAATAATATTGAT+GGG	-	chr7.2:53051274-53051293	MS.gene010899:CDS	25.0%
!!!	TTTTAATTTAATGGCGTGAT+TGG	-	chr7.2:53051123-53051142	MS.gene010899:CDS	25.0%
	AGAATCTTAAATAGGTCAAC+AGG	+	chr7.2:53050810-53050829	None:intergenic	30.0%
	CATCCACAAGAATCTTAAAT+AGG	+	chr7.2:53050818-53050837	None:intergenic	30.0%
	GTGAAGAGCAATAATATTGA+TGG	-	chr7.2:53051273-53051292	MS.gene010899:CDS	30.0%
!	GATTGGTGTAGTTTGTAATA+TGG	-	chr7.2:53051140-53051159	MS.gene010899:CDS	30.0%
!	TGACCTATTTAAGATTCTTG+TGG	-	chr7.2:53050812-53050831	MS.gene010899:CDS	30.0%
	AAAGATAGGGTTAACGAAGA+GGG	+	chr7.2:53050985-53051004	None:intergenic	35.0%
	CAAAAAGATGAAGTATGAGG+AGG	-	chr7.2:53051248-53051267	MS.gene010899:CDS	35.0%
	GAGCAATAATATTGATGGGA+AGG	-	chr7.2:53051278-53051297	MS.gene010899:CDS	35.0%
	GGTTTCATCGTCTTTGATAT+TGG	-	chr7.2:53050924-53050943	MS.gene010899:CDS	35.0%
	TCTTCGTTAACCCTATCTTT+CGG	-	chr7.2:53050985-53051004	MS.gene010899:intron	35.0%
	AAACCCTAGGAGTTTATCCT+CGG	+	chr7.2:53050898-53050917	None:intergenic	40.0%
	AAACTCCTAGGGTTTATCCT+CGG	-	chr7.2:53050903-53050922	MS.gene010899:CDS	40.0%
	AACCCTATCTTTCGGAGAAA+AGG	-	chr7.2:53050993-53051012	MS.gene010899:intron	40.0%
	AACCTTGTCCAAGTCCATTT+CGG	+	chr7.2:53051045-53051064	None:intergenic	40.0%
	CTGTTACTGTGTTTGTAGCA+AGG	-	chr7.2:53051168-53051187	MS.gene010899:CDS	40.0%
	GAAAGATAGGGTTAACGAAG+AGG	+	chr7.2:53050986-53051005	None:intergenic	40.0%
	GATGCTAAAAGTTTGGCGAA+TGG	-	chr7.2:53050855-53050874	MS.gene010899:CDS	40.0%
	TAAGAGTAATGCGTCAGTGT+TGG	+	chr7.2:53051226-53051245	None:intergenic	40.0%
	TATCAAAGACGATGAAACCG+AGG	+	chr7.2:53050923-53050942	None:intergenic	40.0%
	TGACGTGGATGCTAAAAGTT+TGG	-	chr7.2:53050848-53050867	MS.gene010899:CDS	40.0%
	TGCTACAAACACAGTAACAG+AGG	+	chr7.2:53051168-53051187	None:intergenic	40.0%
!	AAACTTTTAGCATCCACGTC+AGG	+	chr7.2:53050849-53050868	None:intergenic	40.0%
!	ATTGGTGATCTGAAGCATAC+TGG	-	chr7.2:53050942-53050961	MS.gene010899:intron	40.0%
!	GATCTGAAGCATACTGGTTA+TGG	-	chr7.2:53050948-53050967	MS.gene010899:intron	40.0%
!	GTCCTTTTCTCCGAAAGATA+GGG	+	chr7.2:53050998-53051017	None:intergenic	40.0%
!	TGTCCTTTTCTCCGAAAGAT+AGG	+	chr7.2:53050999-53051018	None:intergenic	40.0%
	ATCATGACGAAGAAGCTCGA+GGG	-	chr7.2:53051315-53051334	MS.gene010899:CDS	45.0%
	ATCTCTTACGACCACATGTG+GGG	+	chr7.2:53050781-53050800	None:intergenic	45.0%
	CATCTCTTACGACCACATGT+GGG	+	chr7.2:53050782-53050801	None:intergenic	45.0%
	TAAGATTCTTCAAGTCGCCG+AGG	-	chr7.2:53050878-53050897	MS.gene010899:CDS	45.0%
	TGAAACCGAGGATAAACCCT+AGG	+	chr7.2:53050911-53050930	None:intergenic	45.0%
!!	AATGCGTCAGTGTTGGTCTT+CGG	+	chr7.2:53051219-53051238	None:intergenic	45.0%
	ACTTGTGACCATGGCCGAAA+TGG	-	chr7.2:53051028-53051047	MS.gene010899:intron	50.0%
	CATCATGACGAAGAAGCTCG+AGG	-	chr7.2:53051314-53051333	MS.gene010899:CDS	50.0%
	GCATCTCTTACGACCACATG+TGG	+	chr7.2:53050783-53050802	None:intergenic	50.0%
	TCATGACGAAGAAGCTCGAG+GGG	-	chr7.2:53051316-53051335	MS.gene010899:CDS	50.0%
	TCGCCGAGGATAAACTCCTA+GGG	-	chr7.2:53050892-53050911	MS.gene010899:CDS	50.0%
!!	ATGCGTCAGTGTTGGTCTTC+GGG	+	chr7.2:53051218-53051237	None:intergenic	50.0%
!!!	GGATGCTGTTTTACCTGACG+TGG	-	chr7.2:53050833-53050852	MS.gene010899:CDS	50.0%
	GTCCAAGTCCATTTCGGCCA+TGG	+	chr7.2:53051039-53051058	None:intergenic	55.0%
	GTCGCCGAGGATAAACTCCT+AGG	-	chr7.2:53050891-53050910	MS.gene010899:CDS	55.0%
!	CGCTACTCGACTTGTGACCA+TGG	-	chr7.2:53051019-53051038	MS.gene010899:intron	55.0%
!	GACCATGGCCGAAATGGACT+TGG	-	chr7.2:53051034-53051053	MS.gene010899:intron	55.0%
!	GGCCGAAATGGACTTGGACA+AGG	-	chr7.2:53051040-53051059	MS.gene010899:intron	55.0%
!!	GTCAGTGTTGGTCTTCGGGA+CGG	+	chr7.2:53051214-53051233	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	53050771	53051359	53050771	ID=MS.gene010899
chr7.2	mRNA	53050771	53051359	53050771	ID=MS.gene010899.t1;Parent=MS.gene010899
chr7.2	exon	53051069	53051359	53051069	ID=MS.gene010899.t1.exon1;Parent=MS.gene010899.t1
chr7.2	CDS	53051069	53051359	53051069	ID=cds.MS.gene010899.t1;Parent=MS.gene010899.t1
chr7.2	exon	53050771	53050944	53050771	ID=MS.gene010899.t1.exon2;Parent=MS.gene010899.t1
chr7.2	CDS	53050771	53050944	53050771	ID=cds.MS.gene010899.t1;Parent=MS.gene010899.t1

Gene Sequence

>MS.gene010899

ATGGAGTCCCCACATGTGGTCGTAAGAGATGCAACACCTGTTGACCTATTTAAGATTCTTGTGGATGCTGTTTTACCTGACGTGGATGCTAAAAGTTTGGCGAATGGTAAGATTCTTCAAGTCGCCGAGGATAAACTCCTAGGGTTTATCCTCGGTTTCATCGTCTTTGATATTGGTGATCTGAAGCATACTGGTTATGGTCATATCACTTCCCTCTTCGTTAACCCTATCTTTCGGAGAAAAGGACACGCTACTCGACTTGTGACCATGGCCGAAATGGACTTGGACAAGGTTTGTTTGTTTTCTACCTATTGACTCTGTCATAATTTTTTTTGTTAACAATTGTGTCTTCTTTTAATTTAATGGCGTGATTGGTGTAGTTTGTAATATGGTTCCTCTGTTACTGTGTTTGTAGCAAGGTGCTGCTTACGTCTCAGTGACCGTCCCGAAGACCAACACTGACGCATTACTCTTATTCAAAAAGATGAAGTATGAGGAGGCTGTGAAGAGCAATAATATTGATGGGAAGGATGATGCTAATGTCATCATGACGAAGAAGCTCGAGGGGAAGCAGATGCATTCTGCGTAG

Protein sequence

>MS.gene010899.t1

MESPHVVVRDATPVDLFKILVDAVLPDVDAKSLANGKILQVAEDKLLGFILGFIVFDIGDLKHTGYGHITSLFVNPIFRRKGHATRLVTMAEMDLDKQGAAYVSVTVPKTNTDALLLFKKMKYEEAVKSNNIDGKDDANVIMTKKLEGKQMHSA