Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010941.t1 | XP_024626636.1 | 90.2 | 224 | 22 | 0 | 3 | 226 | 62 | 285 | 9.20E-107 | 396.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010941.t1 | Q8L3W1 | 34.4 | 131 | 79 | 4 | 73 | 203 | 213 | 336 | 1.2e-09 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010941.t1 | G7KVV6 | 90.2 | 224 | 22 | 0 | 3 | 226 | 90 | 313 | 6.6e-107 | 396.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene010941.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010941.t1 | MTR_7g050580 | 89.381 | 226 | 24 | 0 | 1 | 226 | 88 | 313 | 4.01e-138 | 390 |
| MS.gene010941.t1 | MTR_7g050710 | 66.525 | 236 | 62 | 3 | 1 | 219 | 88 | 323 | 2.00e-101 | 297 |
| MS.gene010941.t1 | MTR_7g050590 | 64.957 | 234 | 63 | 5 | 1 | 216 | 61 | 293 | 1.12e-100 | 294 |
| MS.gene010941.t1 | MTR_3g098790 | 64.474 | 228 | 72 | 2 | 1 | 219 | 88 | 315 | 3.45e-100 | 294 |
| MS.gene010941.t1 | MTR_7g050560 | 61.570 | 242 | 61 | 6 | 1 | 226 | 88 | 313 | 3.06e-93 | 276 |
| MS.gene010941.t1 | MTR_7g050720 | 49.573 | 234 | 88 | 9 | 1 | 205 | 85 | 317 | 1.56e-62 | 197 |
| MS.gene010941.t1 | MTR_1g035460 | 30.588 | 255 | 129 | 8 | 1 | 220 | 61 | 302 | 4.54e-21 | 90.5 |
| MS.gene010941.t1 | MTR_1g108780 | 29.388 | 245 | 119 | 9 | 1 | 210 | 72 | 297 | 6.76e-20 | 87.8 |
| MS.gene010941.t1 | MTR_8g103940 | 32.051 | 234 | 112 | 10 | 1 | 203 | 114 | 331 | 8.51e-19 | 84.7 |
| MS.gene010941.t1 | MTR_5g044670 | 30.531 | 226 | 133 | 8 | 3 | 211 | 93 | 311 | 2.03e-15 | 74.3 |
| MS.gene010941.t1 | MTR_4g068320 | 32.331 | 133 | 84 | 2 | 76 | 208 | 267 | 393 | 1.18e-14 | 72.4 |
| MS.gene010941.t1 | MTR_1g034240 | 30.147 | 136 | 89 | 3 | 73 | 208 | 268 | 397 | 7.79e-14 | 70.1 |
| MS.gene010941.t1 | MTR_1g035490 | 34.959 | 123 | 73 | 4 | 83 | 203 | 79 | 196 | 5.62e-12 | 64.3 |
| MS.gene010941.t1 | MTR_1g035570 | 30.000 | 210 | 126 | 7 | 8 | 203 | 85 | 287 | 3.79e-11 | 62.4 |
| MS.gene010941.t1 | MTR_1g021330 | 30.612 | 147 | 93 | 4 | 49 | 195 | 215 | 352 | 5.62e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010941.t1 | AT1G49480 | 34.400 | 125 | 75 | 4 | 79 | 203 | 104 | 221 | 1.41e-14 | 70.5 |
| MS.gene010941.t1 | AT1G49480 | 34.400 | 125 | 75 | 4 | 79 | 203 | 104 | 221 | 1.41e-14 | 70.5 |
| MS.gene010941.t1 | AT1G49480 | 34.400 | 125 | 75 | 4 | 79 | 203 | 104 | 221 | 1.41e-14 | 70.5 |
| MS.gene010941.t1 | AT3G18990 | 34.328 | 134 | 81 | 4 | 73 | 206 | 213 | 339 | 8.38e-14 | 70.1 |
| MS.gene010941.t1 | AT3G18990 | 34.351 | 131 | 79 | 4 | 73 | 203 | 213 | 336 | 2.20e-13 | 68.6 |
Find 27 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTAACTCCTTTGAATT+TGG | 0.175508 | 7.1:-51675098 | MS.gene010941:CDS |
| CAAGGAAATGAATCTCTAAA+TGG | 0.329233 | 7.1:-51675149 | MS.gene010941:CDS |
| ATGTAGCTTCTTCTTTCATT+TGG | 0.329258 | 7.1:+51674074 | None:intergenic |
| AGTAGTAGTTGTGTTAAAGT+TGG | 0.371498 | 7.1:-51675074 | MS.gene010941:CDS |
| AGATTCATTTCCTTGATCAT+TGG | 0.386341 | 7.1:+51675157 | None:intergenic |
| AGGTGTTAAATGAAGGAGTT+TGG | 0.387476 | 7.1:-51674394 | MS.gene010941:CDS |
| TGGTAATAGACACTTTGATT+TGG | 0.391800 | 7.1:-51674444 | MS.gene010941:CDS |
| CAGAATATACCACGTATGTT+TGG | 0.412403 | 7.1:-51674464 | MS.gene010941:CDS |
| GTAGTAGTTGTGTTAAAGTT+GGG | 0.429796 | 7.1:-51675073 | MS.gene010941:CDS |
| ATGAAGGAGTTTGGCCTACA+AGG | 0.459083 | 7.1:-51674385 | MS.gene010941:CDS |
| CGCATGACAACAACAAATGA+TGG | 0.473085 | 7.1:+51674822 | None:intergenic |
| TTTGATTTGGATAAGAAAAG+AGG | 0.493087 | 7.1:-51674431 | MS.gene010941:CDS |
| ATGAAAGAAGAAGCTACATA+AGG | 0.494645 | 7.1:-51674070 | None:intergenic |
| TCTATCAGGAAGTCCGGTAC+AGG | 0.500433 | 7.1:-51674359 | MS.gene010941:CDS |
| ATCAAATTGTAGTCCTGTAC+CGG | 0.507873 | 7.1:+51674346 | None:intergenic |
| GAGGACAATAACTTGAAAGT+TGG | 0.523457 | 7.1:-51674296 | MS.gene010941:CDS |
| TCTAAAGATATGAACAATGA+AGG | 0.524509 | 7.1:+51674232 | None:intergenic |
| TTCCTGATAGAAAACCTTGT+AGG | 0.540464 | 7.1:+51674371 | None:intergenic |
| ACTACTACTTCCAAATTCAA+AGG | 0.556173 | 7.1:+51675088 | None:intergenic |
| TGATATCACAAGTGGATGGA+AGG | 0.558355 | 7.1:-51674327 | MS.gene010941:CDS |
| TGTTGAACATAGATTTCTGA+TGG | 0.558556 | 7.1:-51674790 | MS.gene010941:intron |
| ATATGTACCTCAAAATTGGA+AGG | 0.569214 | 7.1:+51675242 | None:intergenic |
| CTACAATTTGATATCACAAG+TGG | 0.598375 | 7.1:-51674335 | MS.gene010941:CDS |
| AATTTGATATCACAAGTGGA+TGG | 0.625506 | 7.1:-51674331 | MS.gene010941:CDS |
| AAATCACAAAAGAAGTCCAA+AGG | 0.633010 | 7.1:-51675050 | MS.gene010941:intron |
| TGGATGGAAGGCATTTGCAG+AGG | 0.673309 | 7.1:-51674315 | MS.gene010941:CDS |
| TGTCTATTACCAAACATACG+TGG | 0.737084 | 7.1:+51674455 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGTAAAAAAAAAAAAAAA+AGG | - | chr7.1:51674375-51674394 | MS.gene010941:CDS | 10.0% |
| !!! | TTTTTTTTTTTTTTTACCTT+TGG | + | chr7.1:51674375-51674394 | None:intergenic | 10.0% |
| !! | ATTTAAAAACAATTTAACTG+TGG | - | chr7.1:51674731-51674750 | MS.gene010941:intron | 15.0% |
| !! | AAAAATATGTACCTCAAAAT+TGG | + | chr7.1:51674171-51674190 | None:intergenic | 20.0% |
| !! | GTTAATGAATAAAAAATGTG+TGG | + | chr7.1:51674900-51674919 | None:intergenic | 20.0% |
| ! | AAGTATGCATTAAATCTAGT+AGG | - | chr7.1:51674432-51674451 | MS.gene010941:CDS | 25.0% |
| ! | TCTAAAGATATGAACAATGA+AGG | + | chr7.1:51675177-51675196 | None:intergenic | 25.0% |
| !! | AAACAATTTAACTGTGGTTT+TGG | - | chr7.1:51674737-51674756 | MS.gene010941:intron | 25.0% |
| !! | CTTTTTATCTTCAACTCTTT+TGG | + | chr7.1:51674225-51674244 | None:intergenic | 25.0% |
| !! | TCTAGTCTTTAGTAGATTAA+GGG | - | chr7.1:51674688-51674707 | MS.gene010941:intron | 25.0% |
| !!! | AAGAGGAGATATTTATTTTC+AGG | - | chr7.1:51674992-51675011 | MS.gene010941:intron | 25.0% |
| !!! | CATTCGTTTTTTCTTTTGTT+TGG | - | chr7.1:51674919-51674938 | MS.gene010941:intron | 25.0% |
| !!! | TATTTTCAGGTGTTAAATGA+AGG | - | chr7.1:51675005-51675024 | MS.gene010941:intron | 25.0% |
| !!! | TTTGATTTGGATAAGAAAAG+AGG | - | chr7.1:51674975-51674994 | MS.gene010941:intron | 25.0% |
| AAAAAGATCTCCAATGATCA+AGG | - | chr7.1:51674239-51674258 | MS.gene010941:CDS | 30.0% | |
| AAAGCTAACTCCTTTGAATT+TGG | - | chr7.1:51674308-51674327 | MS.gene010941:CDS | 30.0% | |
| AAATCACAAAAGAAGTCCAA+AGG | - | chr7.1:51674356-51674375 | MS.gene010941:CDS | 30.0% | |
| AATTTGATATCACAAGTGGA+TGG | - | chr7.1:51675075-51675094 | MS.gene010941:CDS | 30.0% | |
| ACTACTACTTCCAAATTCAA+AGG | + | chr7.1:51674321-51674340 | None:intergenic | 30.0% | |
| AGATTCATTTCCTTGATCAT+TGG | + | chr7.1:51674252-51674271 | None:intergenic | 30.0% | |
| AGTAGTAGTTGTGTTAAAGT+TGG | - | chr7.1:51674332-51674351 | MS.gene010941:CDS | 30.0% | |
| ATATGTACCTCAAAATTGGA+AGG | + | chr7.1:51674167-51674186 | None:intergenic | 30.0% | |
| ATGTAGCTTCTTCTTTCATT+TGG | + | chr7.1:51675335-51675354 | None:intergenic | 30.0% | |
| CAAGGAAATGAATCTCTAAA+TGG | - | chr7.1:51674257-51674276 | MS.gene010941:CDS | 30.0% | |
| CATATCTAAAAAGCAGAAGA+TGG | + | chr7.1:51674139-51674158 | None:intergenic | 30.0% | |
| CTAAAGACTAGAGAAAGATT+TGG | + | chr7.1:51674680-51674699 | None:intergenic | 30.0% | |
| CTACAATTTGATATCACAAG+TGG | - | chr7.1:51675071-51675090 | MS.gene010941:CDS | 30.0% | |
| GTAGTAGTTGTGTTAAAGTT+GGG | - | chr7.1:51674333-51674352 | MS.gene010941:CDS | 30.0% | |
| TGCAAAATTGGAACATTATC+AGG | + | chr7.1:51674492-51674511 | None:intergenic | 30.0% | |
| TGGTAATAGACACTTTGATT+TGG | - | chr7.1:51674962-51674981 | MS.gene010941:intron | 30.0% | |
| TGTTGAACATAGATTTCTGA+TGG | - | chr7.1:51674616-51674635 | MS.gene010941:intron | 30.0% | |
| ! | CTAGAGAAAGATTTGGTTAT+TGG | + | chr7.1:51674673-51674692 | None:intergenic | 30.0% |
| ! | CTAGTCTTTAGTAGATTAAG+GGG | - | chr7.1:51674689-51674708 | MS.gene010941:intron | 30.0% |
| ! | CTCTAGTCTTTAGTAGATTA+AGG | - | chr7.1:51674687-51674706 | MS.gene010941:intron | 30.0% |
| ! | TGAAAAACCTTCCAATTTTG+AGG | - | chr7.1:51674157-51674176 | MS.gene010941:intron | 30.0% |
| ! | TTATCTTCAACTCTTTTGGA+AGG | + | chr7.1:51674221-51674240 | None:intergenic | 30.0% |
| !! | TATCTTCAACTCTTTTGGAA+GGG | + | chr7.1:51674220-51674239 | None:intergenic | 30.0% |
| !! | TATTTTTGAAAAGAGTGCGT+TGG | - | chr7.1:51674184-51674203 | MS.gene010941:intron | 30.0% |
| !! | TGATAATGTTCCAATTTTGC+AGG | - | chr7.1:51674491-51674510 | MS.gene010941:intron | 30.0% |
| AGGTATGGTTTAAAAAGGGT+TGG | - | chr7.1:51674078-51674097 | MS.gene010941:CDS | 35.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | - | chr7.1:51675012-51675031 | MS.gene010941:intron | 35.0% | |
| ATCAAATTGTAGTCCTGTAC+CGG | + | chr7.1:51675063-51675082 | None:intergenic | 35.0% | |
| CAGAATATACCACGTATGTT+TGG | - | chr7.1:51674942-51674961 | MS.gene010941:intron | 35.0% | |
| GAGGACAATAACTTGAAAGT+TGG | - | chr7.1:51675110-51675129 | MS.gene010941:CDS | 35.0% | |
| GGCAAGGTATGGTTTAAAAA+GGG | - | chr7.1:51674074-51674093 | MS.gene010941:CDS | 35.0% | |
| TGGCAAGGTATGGTTTAAAA+AGG | - | chr7.1:51674073-51674092 | MS.gene010941:CDS | 35.0% | |
| TGTCTATTACCAAACATACG+TGG | + | chr7.1:51674954-51674973 | None:intergenic | 35.0% | |
| TTCCTGATAGAAAACCTTGT+AGG | + | chr7.1:51675038-51675057 | None:intergenic | 35.0% | |
| !! | CACTTGTTTTCCTGCAAAAT+TGG | + | chr7.1:51674504-51674523 | None:intergenic | 35.0% |
| CGCATGACAACAACAAATGA+TGG | + | chr7.1:51674587-51674606 | None:intergenic | 40.0% | |
| CTCAGAGTGTGTTTCTCTTA+AGG | - | chr7.1:51674768-51674787 | MS.gene010941:intron | 40.0% | |
| CTTAAGAGAAACACACTCTG+AGG | + | chr7.1:51674770-51674789 | None:intergenic | 40.0% | |
| GTCTCAGGTAAGATTTACCT+CGG | - | chr7.1:51674790-51674809 | MS.gene010941:intron | 40.0% | |
| TGATATCACAAGTGGATGGA+AGG | - | chr7.1:51675079-51675098 | MS.gene010941:CDS | 40.0% | |
| TGGAGAGATTCTGCATAAGA+TGG | + | chr7.1:51675315-51675334 | None:intergenic | 40.0% | |
| TGTGTTTCTCTTAAGGTCTC+AGG | - | chr7.1:51674775-51674794 | MS.gene010941:intron | 40.0% | |
| ! | AGGTTTTCTATCAGGAAGTC+CGG | - | chr7.1:51675041-51675060 | MS.gene010941:intron | 40.0% |
| !! | GATTTACCTCGGTGCAATTT+TGG | - | chr7.1:51674801-51674820 | MS.gene010941:CDS | 40.0% |
| ATACCACCAAAATTGCACCG+AGG | + | chr7.1:51674810-51674829 | None:intergenic | 45.0% | |
| ATGAAGGAGTTTGGCCTACA+AGG | - | chr7.1:51675021-51675040 | MS.gene010941:intron | 45.0% | |
| ! | GGCCTACAAGGTTTTCTATC+AGG | - | chr7.1:51675033-51675052 | MS.gene010941:intron | 45.0% |
| !! | TTACCTCGGTGCAATTTTGG+TGG | - | chr7.1:51674804-51674823 | MS.gene010941:CDS | 45.0% |
| TCTATCAGGAAGTCCGGTAC+AGG | - | chr7.1:51675047-51675066 | MS.gene010941:intron | 50.0% | |
| ! | GAGCATCACTCTCTAGCTCA+TGG | - | chr7.1:51674113-51674132 | MS.gene010941:intron | 50.0% |
| !! | TGGATGGAAGGCATTTGCAG+AGG | - | chr7.1:51675091-51675110 | MS.gene010941:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 51674072 | 51675356 | 51674072 | ID=MS.gene010941 |
| chr7.1 | mRNA | 51674072 | 51675356 | 51674072 | ID=MS.gene010941.t1;Parent=MS.gene010941 |
| chr7.1 | exon | 51675349 | 51675356 | 51675349 | ID=MS.gene010941.t1.exon1;Parent=MS.gene010941.t1 |
| chr7.1 | CDS | 51675349 | 51675356 | 51675349 | ID=cds.MS.gene010941.t1;Parent=MS.gene010941.t1 |
| chr7.1 | exon | 51675051 | 51675274 | 51675051 | ID=MS.gene010941.t1.exon2;Parent=MS.gene010941.t1 |
| chr7.1 | CDS | 51675051 | 51675274 | 51675051 | ID=cds.MS.gene010941.t1;Parent=MS.gene010941.t1 |
| chr7.1 | exon | 51674791 | 51674915 | 51674791 | ID=MS.gene010941.t1.exon3;Parent=MS.gene010941.t1 |
| chr7.1 | CDS | 51674791 | 51674915 | 51674791 | ID=cds.MS.gene010941.t1;Parent=MS.gene010941.t1 |
| chr7.1 | exon | 51674192 | 51674483 | 51674192 | ID=MS.gene010941.t1.exon4;Parent=MS.gene010941.t1 |
| chr7.1 | CDS | 51674192 | 51674483 | 51674192 | ID=cds.MS.gene010941.t1;Parent=MS.gene010941.t1 |
| chr7.1 | exon | 51674072 | 51674103 | 51674072 | ID=MS.gene010941.t1.exon5;Parent=MS.gene010941.t1 |
| chr7.1 | CDS | 51674072 | 51674103 | 51674072 | ID=cds.MS.gene010941.t1;Parent=MS.gene010941.t1 |
| Gene Sequence |
| Protein sequence |
