AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010944


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010944.t1 MTR_7g050710 89.474 95 10 0 1 95 1 95 8.00e-60 186
MS.gene010944.t1 MTR_7g050580 76.923 104 24 0 1 104 1 104 8.30e-55 173
MS.gene010944.t1 MTR_3g098790 73.404 94 25 0 1 94 1 94 6.22e-48 155
MS.gene010944.t1 MTR_7g050560 69.231 104 32 0 1 104 1 104 8.44e-47 152
MS.gene010944.t1 MTR_7g050640 59.615 104 42 0 1 104 1 104 1.05e-40 130
MS.gene010944.t1 MTR_7g050720 68.132 91 29 0 7 97 4 94 1.43e-39 134
MS.gene010944.t1 MTR_7g050590 77.143 70 16 0 28 97 1 70 9.89e-35 120
MS.gene010944.t1 MTR_3g061290 51.765 85 39 1 10 94 9 91 4.83e-25 93.6
MS.gene010944.t1 MTR_8g103940 44.318 88 49 0 11 98 37 124 6.74e-24 94.7
MS.gene010944.t1 MTR_3g061330 47.059 85 43 1 10 94 9 91 1.83e-22 88.2
MS.gene010944.t1 MTR_3g061320 49.398 83 40 1 10 92 9 89 2.57e-22 88.2
MS.gene010944.t1 MTR_3g061310 45.882 85 44 1 10 94 9 91 1.05e-21 86.7
MS.gene010944.t1 MTR_3g061340 46.429 84 43 1 10 93 9 90 2.13e-21 85.9
MS.gene010944.t1 MTR_3g061370 44.444 90 48 1 5 94 1 88 2.36e-21 85.9
MS.gene010944.t1 MTR_5g044670 43.750 80 45 0 13 92 16 95 1.70e-20 84.3
MS.gene010944.t1 MTR_3g061350 43.617 94 50 2 1 94 1 91 8.20e-20 81.6
MS.gene010944.t1 MTR_1g108780 47.297 74 36 1 21 94 8 78 9.07e-19 80.1
MS.gene010944.t1 MTR_1g050845 41.758 91 51 1 10 100 6 94 1.56e-16 73.9
MS.gene010944.t1 MTR_1g035460 41.791 67 39 0 28 94 1 67 5.93e-16 72.0
MS.gene010944.t1 MTR_1g034240 36.471 85 54 0 13 97 7 91 7.47e-16 72.0
MS.gene010944.t1 MTR_1g035620 40.659 91 52 1 10 100 6 94 1.59e-15 70.9
MS.gene010944.t1 MTR_3g005480 31.818 88 60 0 5 92 13 100 2.09e-15 69.7
MS.gene010944.t1 MTR_3g061220 38.824 85 37 2 10 94 9 78 3.98e-15 66.2
MS.gene010944.t1 MTR_4g068320 33.750 80 53 0 13 92 6 85 7.41e-15 68.9
MS.gene010944.t1 MTR_1g021400 34.568 81 53 0 11 91 28 108 2.24e-14 67.8
MS.gene010944.t1 MTR_4g119990 40.000 85 50 1 11 94 13 97 3.20e-14 67.0
MS.gene010944.t1 MTR_1g021320 34.568 81 53 0 11 91 23 103 6.55e-14 66.6
MS.gene010944.t1 MTR_1g021320 34.940 83 51 1 12 91 178 260 2.07e-11 59.3
MS.gene010944.t1 MTR_3g005490 30.864 81 56 0 12 92 20 100 1.24e-13 65.1
MS.gene010944.t1 MTR_1g021440 34.568 81 52 1 11 91 21 100 1.38e-13 65.1
MS.gene010944.t1 MTR_1g021440 34.568 81 52 1 11 91 21 100 1.70e-13 64.7
MS.gene010944.t1 MTR_1g034210 36.471 85 54 0 13 97 7 91 1.95e-13 65.1
MS.gene010944.t1 MTR_1g021440 34.568 81 52 1 11 91 21 100 3.88e-13 64.3
MS.gene010944.t1 MTR_1g021440 34.568 81 52 1 11 91 21 100 3.94e-13 63.9
MS.gene010944.t1 MTR_1g034210 36.471 85 54 0 13 97 7 91 3.96e-13 63.9
MS.gene010944.t1 MTR_1g035480 40.476 84 47 2 8 91 12 92 4.92e-13 62.0
MS.gene010944.t1 MTR_1g021380 34.568 81 52 1 11 91 20 99 6.77e-13 63.5
MS.gene010944.t1 MTR_6g043890 38.095 84 51 1 11 93 13 96 1.12e-12 62.8
MS.gene010944.t1 MTR_3g005460 33.000 100 63 2 2 97 248 347 1.44e-12 62.8
MS.gene010944.t1 MTR_3g005460 31.034 87 60 0 6 92 18 104 7.98e-11 57.8
MS.gene010944.t1 MTR_3g005495 35.955 89 55 1 4 92 236 322 2.06e-12 62.0
MS.gene010944.t1 MTR_1g035640 42.500 80 40 2 12 91 8 81 2.77e-12 61.6
MS.gene010944.t1 MTR_1g035500 36.782 87 52 2 6 92 18 101 3.18e-12 60.1
MS.gene010944.t1 MTR_1g035650 37.349 83 50 1 10 92 6 86 3.39e-12 60.5
MS.gene010944.t1 MTR_3g005420 33.708 89 57 1 4 92 282 368 3.40e-12 61.6
MS.gene010944.t1 MTR_1g021435 32.099 81 55 0 11 91 20 100 5.98e-12 60.8
MS.gene010944.t1 MTR_5g070440 28.395 81 58 0 12 92 20 100 2.22e-11 58.9
MS.gene010944.t1 MTR_6g061070 37.931 87 51 2 6 92 11 94 2.92e-11 58.9
MS.gene010944.t1 MTR_1g021410 32.099 81 54 1 11 91 21 100 3.68e-11 58.5
MS.gene010944.t1 MTR_1g021360 34.940 83 51 1 12 91 178 260 3.73e-11 58.5
MS.gene010944.t1 MTR_1g021350 32.099 81 54 1 11 91 20 99 3.76e-11 58.5
MS.gene010944.t1 MTR_1g021290 33.333 78 51 1 14 91 157 233 4.14e-11 58.2
MS.gene010944.t1 MTR_1g021330 30.864 81 56 0 11 91 24 104 7.38e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010944.t1 AT3G18990 40.000 80 48 0 13 92 6 85 1.70e-17 76.3
MS.gene010944.t1 AT3G18990 40.000 80 48 0 13 92 6 85 2.07e-17 75.9
MS.gene010944.t1 AT1G49475 38.372 86 52 1 13 98 34 118 2.13e-16 71.2
MS.gene010944.t1 AT3G18960 37.209 86 54 0 13 98 30 115 3.81e-16 70.5
MS.gene010944.t1 AT3G18960 37.209 86 54 0 13 98 30 115 4.51e-16 70.5
MS.gene010944.t1 AT4G01580 37.209 86 54 0 13 98 30 115 4.57e-16 70.1
MS.gene010944.t1 AT3G18960 41.429 70 41 0 29 98 9 78 9.58e-14 63.9

Find 19 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATTTGTACCTCAAATT+TGG 0.160650 7.1:+51590528 None:intergenic
TGCAAAATGGAAATTAAATT+TGG 0.185593 7.1:-51590655 MS.gene010944:CDS
AAATTTGAGGTACAAATCTT+TGG 0.251414 7.1:-51590526 MS.gene010944:CDS
GTCTCTAGACATATAGCTTT+TGG 0.313968 7.1:+51590687 None:intergenic
AGACTCCAAATGGTGCAAAA+TGG 0.357064 7.1:-51590668 MS.gene010944:CDS
AAAGGCTTACAAGGTTTCCT+TGG 0.416023 7.1:+51591378 None:intergenic
CAATGAGTGATGGCAAGATA+TGG 0.428994 7.1:-51590632 MS.gene010944:CDS
AATCTTTGGAAAGAGTGCTT+TGG 0.429279 7.1:-51590512 MS.gene010944:CDS
GATGATGCCAAGAAAGTTTG+TGG 0.433802 7.1:-51590730 MS.gene010944:CDS
AGATATGGTTTGAGAAGGGT+TGG 0.481735 7.1:-51590617 MS.gene010944:CDS
TTAAATTTGGCAATGAGTGA+TGG 0.512239 7.1:-51590642 MS.gene010944:CDS
TTACAAGGTTTCCTTGGAGA+AGG 0.514232 7.1:+51591384 None:intergenic
TTTGCAGAGCATCACTCTCT+AGG 0.540785 7.1:-51590588 MS.gene010944:CDS
TGGCAAGATATGGTTTGAGA+AGG 0.555350 7.1:-51590622 MS.gene010944:CDS
ATATGTCTAGAGACTCCAAA+TGG 0.586498 7.1:-51590678 MS.gene010944:CDS
GAGCATCACTCTCTAGGTCA+TGG 0.595626 7.1:-51590582 MS.gene010944:CDS
GGCAAGATATGGTTTGAGAA+GGG 0.606783 7.1:-51590621 MS.gene010944:CDS
GTTACTCAAAACCTTCTCCA+AGG 0.614774 7.1:-51591395 MS.gene010944:CDS
CATGGCTAGTGAAATTGCTG+AGG 0.726199 7.1:-51591447 MS.gene010944:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATCATTCATATTAATA+TGG - chr7.1:51591184-51591203 MS.gene010944:intron 10.0%
!!! ATATTATTTTACATTACTTT+AGG + chr7.1:51591160-51591179 None:intergenic 10.0%
!!! TTAAAAAAAGCATAAAAATA+AGG + chr7.1:51590881-51590900 None:intergenic 10.0%
!! AAAACAAGTAACTTCAAATT+TGG - chr7.1:51590736-51590755 MS.gene010944:intron 20.0%
!! ATTCAATTGTTCTGTAATTT+AGG + chr7.1:51590833-51590852 None:intergenic 20.0%
!! TGCAAAATGGAAATTAAATT+TGG - chr7.1:51591297-51591316 MS.gene010944:intron 20.0%
! AAAGATTTGTACCTCAAATT+TGG + chr7.1:51591427-51591446 None:intergenic 25.0%
! AACATCAACAAAGAATCTAA+AGG + chr7.1:51590595-51590614 None:intergenic 25.0%
! AGAAAGTTTGTGGAAAAATA+TGG - chr7.1:51591232-51591251 MS.gene010944:intron 25.0%
! ATGAAAAAACTCCAAATTTG+AGG - chr7.1:51591413-51591432 MS.gene010944:CDS 25.0%
! ATGGGACAAATTGAATAAAT+TGG + chr7.1:51591101-51591120 None:intergenic 25.0%
! TAACTATGATCTTGAAAAAG+TGG + chr7.1:51590541-51590560 None:intergenic 25.0%
! TTTATTCAATTTGTCCCATT+TGG - chr7.1:51591102-51591121 MS.gene010944:intron 25.0%
! TTTGTGGAAAAATATGGAAA+AGG - chr7.1:51591238-51591257 MS.gene010944:intron 25.0%
!! AAATTTGAGGTACAAATCTT+TGG - chr7.1:51591426-51591445 MS.gene010944:CDS 25.0%
!! CGATTACTTGTTAAAAACTT+GGG - chr7.1:51591028-51591047 MS.gene010944:intron 25.0%
!! TACATTACTTTAGGAAATAC+TGG + chr7.1:51591151-51591170 None:intergenic 25.0%
!! TATTTTTCCACAAACTTTCT+TGG + chr7.1:51591232-51591251 None:intergenic 25.0%
!! TCGATTACTTGTTAAAAACT+TGG - chr7.1:51591027-51591046 MS.gene010944:intron 25.0%
!!! TTTTAAGAACGTTTCTCTTT+AGG - chr7.1:51590895-51590914 MS.gene010944:intron 25.0%
AAAGAATCTAAAGGCTTACA+AGG + chr7.1:51590586-51590605 None:intergenic 30.0%
AAGAAACCTAAATACACATC+AGG + chr7.1:51590991-51591010 None:intergenic 30.0%
ATGATCTTGAAAAAGTGGAT+TGG + chr7.1:51590536-51590555 None:intergenic 30.0%
CATATCTAAAAAGCAGAAGA+TGG + chr7.1:51591396-51591415 None:intergenic 30.0%
TAACTACCTGATGTGTATTT+AGG - chr7.1:51590982-51591001 MS.gene010944:intron 30.0%
TACCAGATTATGTTTATTCC+AGG + chr7.1:51590960-51590979 None:intergenic 30.0%
TCCAGGAGTTAATTGTATTA+GGG + chr7.1:51590943-51590962 None:intergenic 30.0%
TTAAATTTGGCAATGAGTGA+TGG - chr7.1:51591310-51591329 MS.gene010944:intron 30.0%
TTCCAGGAGTTAATTGTATT+AGG + chr7.1:51590944-51590963 None:intergenic 30.0%
! AACAATTGAATCATTTTCGC+CGG - chr7.1:51590842-51590861 MS.gene010944:intron 30.0%
! AATTTCCATTTTGCACCATT+TGG + chr7.1:51591292-51591311 None:intergenic 30.0%
! TAAAACAGAGCTGTCAATTT+TGG - chr7.1:51590786-51590805 MS.gene010944:intron 30.0%
!! TCAATTTTGGTCAAACCTAA+TGG - chr7.1:51590799-51590818 MS.gene010944:intron 30.0%
AGAAAAGAATAGAGAGGTTC+AGG + chr7.1:51590710-51590729 None:intergenic 35.0%
ATATGTCTAGAGACTCCAAA+TGG - chr7.1:51591274-51591293 MS.gene010944:intron 35.0%
CAGGAGTTAATTGTATTAGG+GGG + chr7.1:51590941-51590960 None:intergenic 35.0%
CCAGGAGTTAATTGTATTAG+GGG + chr7.1:51590942-51590961 None:intergenic 35.0%
CCTGTTAGAAAAGAATAGAG+AGG + chr7.1:51590716-51590735 None:intergenic 35.0%
CTCCTGGAATAAACATAATC+TGG - chr7.1:51590955-51590974 MS.gene010944:intron 35.0%
! CCTCTCTATTCTTTTCTAAC+AGG - chr7.1:51590713-51590732 MS.gene010944:CDS 35.0%
! GTCTCTAGACATATAGCTTT+TGG + chr7.1:51591268-51591287 None:intergenic 35.0%
!! AATCTTTGGAAAGAGTGCTT+TGG - chr7.1:51591440-51591459 MS.gene010944:CDS 35.0%
AAAGGCTTACAAGGTTTCCT+TGG + chr7.1:51590577-51590596 None:intergenic 40.0%
AATTTGTCCCATTTGGCTAG+GGG - chr7.1:51591109-51591128 MS.gene010944:intron 40.0%
AGAATAGAGAGGTTCAGGTA+AGG + chr7.1:51590705-51590724 None:intergenic 40.0%
AGACTCCAAATGGTGCAAAA+TGG - chr7.1:51591284-51591303 MS.gene010944:intron 40.0%
AGATATGGTTTGAGAAGGGT+TGG - chr7.1:51591335-51591354 MS.gene010944:intron 40.0%
AGCCTAAAACACACCAGTTT+GGG + chr7.1:51591064-51591083 None:intergenic 40.0%
CAATGAGTGATGGCAAGATA+TGG - chr7.1:51591320-51591339 MS.gene010944:intron 40.0%
CAATTTGTCCCATTTGGCTA+GGG - chr7.1:51591108-51591127 MS.gene010944:intron 40.0%
CCCCTAATACAATTAACTCC+TGG - chr7.1:51590939-51590958 MS.gene010944:intron 40.0%
GATGATGCCAAGAAAGTTTG+TGG - chr7.1:51591222-51591241 MS.gene010944:intron 40.0%
GGCAAGATATGGTTTGAGAA+GGG - chr7.1:51591331-51591350 MS.gene010944:intron 40.0%
GTTACTCAAAACCTTCTCCA+AGG - chr7.1:51590557-51590576 MS.gene010944:CDS 40.0%
TAGCCTAAAACACACCAGTT+TGG + chr7.1:51591065-51591084 None:intergenic 40.0%
TCAATTTGTCCCATTTGGCT+AGG - chr7.1:51591107-51591126 MS.gene010944:intron 40.0%
TGGCAAGATATGGTTTGAGA+AGG - chr7.1:51591330-51591349 MS.gene010944:intron 40.0%
TTACAAGGTTTCCTTGGAGA+AGG + chr7.1:51590571-51590590 None:intergenic 40.0%
! AAAACACACCAGTTTGGGTT+TGG + chr7.1:51591059-51591078 None:intergenic 40.0%
! AACCCAAACTGGTGTGTTTT+AGG - chr7.1:51591059-51591078 MS.gene010944:intron 40.0%
! ATTGAATCATTTTCGCCGGT+TGG - chr7.1:51590846-51590865 MS.gene010944:intron 40.0%
! ATTTAGGAGTGCAGTCCATT+AGG + chr7.1:51590817-51590836 None:intergenic 40.0%
ACAAACTCCCCTAGCCAAAT+GGG + chr7.1:51591119-51591138 None:intergenic 45.0%
AGGCCCCACAAATATCTCTA+AGG - chr7.1:51590915-51590934 MS.gene010944:intron 45.0%
GGGCCTTAGAGATATTTGTG+GGG + chr7.1:51590921-51590940 None:intergenic 45.0%
GGGGCCTTAGAGATATTTGT+GGG + chr7.1:51590922-51590941 None:intergenic 45.0%
! TTTGCAGAGCATCACTCTCT+AGG - chr7.1:51591364-51591383 MS.gene010944:intron 45.0%
ATAAGGCCGAGTGAACCAAC+CGG + chr7.1:51590864-51590883 None:intergenic 50.0%
CACAAACTCCCCTAGCCAAA+TGG + chr7.1:51591120-51591139 None:intergenic 50.0%
GAGCATCACTCTCTAGGTCA+TGG - chr7.1:51591370-51591389 MS.gene010944:intron 50.0%
GGGGGCCTTAGAGATATTTG+TGG + chr7.1:51590923-51590942 None:intergenic 50.0%
TTTGGCTAGGGGAGTTTGTG+TGG - chr7.1:51591120-51591139 MS.gene010944:intron 50.0%
!! TTTTCGCCGGTTGGTTCACT+CGG - chr7.1:51590855-51590874 MS.gene010944:intron 50.0%
GGGCTTAGCCAAACCCAAAC+TGG - chr7.1:51591048-51591067 MS.gene010944:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 51590506 51591468 51590506 ID=MS.gene010944
chr7.1 mRNA 51590506 51591468 51590506 ID=MS.gene010944.t1;Parent=MS.gene010944
chr7.1 exon 51591388 51591468 51591388 ID=MS.gene010944.t1.exon1;Parent=MS.gene010944.t1
chr7.1 CDS 51591388 51591468 51591388 ID=cds.MS.gene010944.t1;Parent=MS.gene010944.t1
chr7.1 exon 51590506 51590751 51590506 ID=MS.gene010944.t1.exon2;Parent=MS.gene010944.t1
chr7.1 CDS 51590506 51590751 51590506 ID=cds.MS.gene010944.t1;Parent=MS.gene010944.t1
Gene Sequence

>MS.gene010944

ATGGCTAGTGAAATTGCTGAGGTGAAGCCAATCCACTTTTTCAAGATCATAGTTACTCAAAACCTTCTCCAAGGAAACCTTGTAAGCCTTTAGATTCTTTGTTGATGTTATTAATGTTTTATTTTTTTTTATAAAGAGCATGTTGTTTATGTTTGTATATCTTATAACATGATTTTACTTTGTATTTGACATATTACCTTACCTGAACCTCTCTATTCTTTTCTAACAGGAAAACAAGTAACTTCAAATTTGGATTTACACTTAGTTTTAGTTGTATATATAAAACAGAGCTGTCAATTTTGGTCAAACCTAATGGACTGCACTCCTAAATTACAGAACAATTGAATCATTTTCGCCGGTTGGTTCACTCGGCCTTATTTTTATGCTTTTTTTAAGAACGTTTCTCTTTAGGCCCCACAAATATCTCTAAGGCCCCCTAATACAATTAACTCCTGGAATAAACATAATCTGGTAGATAACTACCTGATGTGTATTTAGGTTTCTTTTTGAAAAAAATATAATCGATTACTTGTTAAAAACTTGGGCTTAGCCAAACCCAAACTGGTGTGTTTTAGGCTATGAACGATTTCTGCCAATTTATTCAATTTGTCCCATTTGGCTAGGGGAGTTTGTGTGGAGCTGCCAGTATTTCCTAAAGTAATGTAAAATAATATTGAGTTATATCATTCATATTAATATGGTGTAATTAATTGACAGATGATGCCAAGAAAGTTTGTGGAAAAATATGGAAAAGGTCTACCAAAAGCTATATGTCTAGAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGCAATGAGTGATGGCAAGATATGGTTTGAGAAGGGTTGGAAACAATTTGCAGAGCATCACTCTCTAGGTCATGGCCATCTTCTGCTTTTTAGATATGAAAAAACTCCAAATTTGAGGTACAAATCTTTGGAAAGAGTGCTTTGGAGATAG

Protein sequence

>MS.gene010944.t1

MASEIAEVKPIHFFKIIVTQNLLQGNLMMPRKFVEKYGKGLPKAICLETPNGAKWKLNLAMSDGKIWFEKGWKQFAEHHSLGHGHLLLFRYEKTPNLRYKSLERVLWR