AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01112

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01112.t1 MTR_2g007930 99.766 427 1 0 1 427 1 427 0.0 886
MS.gene01112.t1 MTR_1g098790 80.230 435 74 5 1 427 1 431 0.0 692
MS.gene01112.t1 MTR_5g020580 55.300 434 169 12 1 427 1 416 1.04e-155 446
MS.gene01112.t1 MTR_2g020280 58.005 381 137 6 49 427 34 393 1.36e-154 443
MS.gene01112.t1 MTR_1g088960 53.241 432 167 10 1 427 1 402 7.50e-154 441
MS.gene01112.t1 MTR_8g077195 52.791 430 182 8 1 427 1 412 8.72e-149 429
MS.gene01112.t1 MTR_1g022370 49.777 448 178 9 1 427 1 422 1.60e-141 410
MS.gene01112.t1 MTR_2g020280 57.143 315 112 6 115 427 1 294 2.96e-119 349
MS.gene01112.t1 MTR_2g076200 48.670 376 145 9 54 427 42 371 1.50e-116 345
MS.gene01112.t1 MTR_8g077620 40.421 428 205 14 1 420 1 386 9.20e-93 285
MS.gene01112.t1 MTR_2g076210 42.632 380 163 11 54 427 37 367 1.31e-92 284
MS.gene01112.t1 MTR_8g077675 44.389 401 178 14 32 420 28 395 2.34e-91 282
MS.gene01112.t1 MTR_8g077650 40.263 380 153 15 49 419 14 328 6.79e-67 216
MS.gene01112.t1 MTR_1g023800 51.656 151 65 4 1 148 1 146 1.14e-43 150
MS.gene01112.t1 MTR_8g077640 43.172 227 97 6 1 207 46 260 2.56e-41 150
MS.gene01112.t1 MTR_8g077640 45.455 99 47 2 320 417 291 383 1.98e-16 80.9
MS.gene01112.t1 MTR_0210s0010 40.404 99 52 2 96 194 8 99 5.47e-15 71.2
MS.gene01112.t1 MTR_2g105390 40.909 110 44 5 323 426 265 359 4.89e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01112.t1 AT1G25280 73.392 451 90 14 1 427 1 445 0.0 612
MS.gene01112.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene01112.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene01112.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene01112.t1 AT1G43640 68.552 442 111 7 1 427 1 429 0.0 605
MS.gene01112.t1 AT1G43640 70.400 375 86 5 66 427 1 363 0.0 535
MS.gene01112.t1 AT2G47900 55.093 432 163 7 1 427 1 406 3.63e-167 475
MS.gene01112.t1 AT2G47900 55.093 432 163 7 1 427 1 406 3.63e-167 475
MS.gene01112.t1 AT2G47900 54.965 433 163 8 1 427 1 407 1.09e-165 472
MS.gene01112.t1 AT2G18280 52.995 434 156 9 1 427 1 393 1.37e-146 422
MS.gene01112.t1 AT2G18280 52.995 434 156 9 1 427 1 393 1.37e-146 422
MS.gene01112.t1 AT1G47270 50.230 434 163 8 1 427 1 388 8.90e-140 405
MS.gene01112.t1 AT1G47270 50.230 434 163 8 1 427 26 413 1.19e-139 405
MS.gene01112.t1 AT1G47270 50.230 434 163 8 1 427 25 412 1.23e-139 405
MS.gene01112.t1 AT3G06380 51.979 379 146 8 54 427 33 380 3.41e-128 375
MS.gene01112.t1 AT5G18680 49.148 411 162 11 32 427 11 389 1.71e-125 369
MS.gene01112.t1 AT1G25280 73.063 271 52 11 174 427 1 267 8.60e-124 360
MS.gene01112.t1 AT1G25280 73.063 271 52 11 174 427 1 267 8.60e-124 360
MS.gene01112.t1 AT1G53320 48.294 381 151 8 51 427 41 379 1.02e-115 343
MS.gene01112.t1 AT5G18680 51.703 323 116 9 113 427 7 297 7.53e-101 302
MS.gene01112.t1 AT3G06380 51.111 315 119 7 117 427 5 288 6.85e-100 300
MS.gene01112.t1 AT1G61940 56.977 86 37 0 57 142 1 86 8.72e-31 119
MS.gene01112.t1 AT1G61940 60.656 61 24 0 57 117 1 61 3.13e-19 87.0

Find 118 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTTGTTAGTTGAAAA+TGG 0.215408 2.1:+74606306 MS.gene01112:intron
ATCTGCAACTAACCTGTTTC+AGG 0.217420 2.1:-74605583 None:intergenic
TACACCAGATCAAACTTTCT+TGG 0.237446 2.1:+74606896 MS.gene01112:intron
ATGACATCAAATAGTAATTC+TGG 0.251135 2.1:-74605416 None:intergenic
CGTGACGTTCGTGATAGTTT+TGG 0.276663 2.1:+74605263 MS.gene01112:CDS
CCCGCATCAAGTGCTGAATT+TGG 0.304909 2.1:-74607127 None:intergenic
CAGTCCCCGGAGGACATATA+TGG 0.310396 2.1:-74606964 None:intergenic
GTGCTTCTGTTTGCCAGTCT+TGG 0.312781 2.1:+74605495 MS.gene01112:CDS
GATGCGGGCGGCTCCGTTCC+CGG 0.316477 2.1:+74607142 MS.gene01112:CDS
CAGAATAATCTTATCATGTT+CGG 0.319403 2.1:-74607476 None:intergenic
GCTTCCACCCAACCACCTGC+TGG 0.321946 2.1:+74607424 MS.gene01112:CDS
GCCGTCATCATCCTCATTAC+AGG 0.338459 2.1:-74607281 None:intergenic
TTGTAATTCAAAATAGTTGC+TGG 0.356421 2.1:+74605381 MS.gene01112:CDS
GTGAATTAGCTCTGGCTGTC+CGG 0.359818 2.1:-74607161 None:intergenic
TCATTCAATGCTTTATCAAA+AGG 0.375284 2.1:+74605728 MS.gene01112:CDS
CATATATGTCCTCCGGGGAC+TGG 0.388613 2.1:+74606965 MS.gene01112:CDS
ACATATATGGGCAGAAGTGC+AGG 0.394714 2.1:-74606951 None:intergenic
GTTAGTTTGCCGCAGAACTC+TGG 0.395072 2.1:-74605548 None:intergenic
TTCTGCAATTCGGCAAAGTT+GGG 0.396243 2.1:+74607494 MS.gene01112:CDS
AATTACTATTTGATGTCATT+CGG 0.397064 2.1:+74605420 MS.gene01112:CDS
AAAGCTTGGAATGCAGATAA+AGG 0.399095 2.1:-74607547 None:intergenic
TTACTCGTCCCCGAAAATTA+AGG 0.402869 2.1:-74607369 None:intergenic
ATTCCCTGTGTCCCTGAAAC+AGG 0.406088 2.1:+74605571 MS.gene01112:CDS
TTCTGCCCATATATGTCCTC+CGG 0.408726 2.1:+74606958 MS.gene01112:CDS
CTTCCACCCAACCACCTGCT+GGG 0.409907 2.1:+74607425 MS.gene01112:CDS
CACTGAGTATGTTATTTCAA+TGG 0.410829 2.1:+74606371 MS.gene01112:CDS
GTGAGAATACTTGGCCTTCA+AGG 0.416059 2.1:+74605456 MS.gene01112:CDS
TCGGCGCCCCAGCAGGTGGT+TGG 0.419959 2.1:-74607432 None:intergenic
CTTCTGTTTGCCAGTCTTGG+AGG 0.420970 2.1:+74605498 MS.gene01112:CDS
TGTAATTGTTCATGCCATCT+TGG 0.429018 2.1:-74607337 None:intergenic
CTCAGTGTAGGTTGTTCTCC+TGG 0.432833 2.1:-74606355 None:intergenic
TAACTCCCAGATGGGACCTT+TGG 0.438868 2.1:-74607022 None:intergenic
GCAACTACATGCTTCCTTGA+AGG 0.442687 2.1:-74605470 None:intergenic
TGCTCCGGAACGAGTCCTCC+AGG 0.444143 2.1:-74607189 None:intergenic
GTATCGCCAAAGGTCCCATC+TGG 0.445185 2.1:+74607016 MS.gene01112:CDS
GATGGTGTCGGCGCCCCAGC+AGG 0.447033 2.1:-74607439 None:intergenic
GATAAGATTATTCTGCAATT+CGG 0.448717 2.1:+74607484 MS.gene01112:CDS
CGGCGCCCCAGCAGGTGGTT+GGG 0.449066 2.1:-74607431 None:intergenic
AGAATAATCTTATCATGTTC+GGG 0.450318 2.1:-74607475 None:intergenic
ATTCTGCAATTCGGCAAAGT+TGG 0.451251 2.1:+74607493 MS.gene01112:CDS
ATATATGTCCTCCGGGGACT+GGG 0.453188 2.1:+74606966 MS.gene01112:CDS
TGACATCAAATAGTAATTCT+GGG 0.457179 2.1:-74605415 None:intergenic
TAATGCAATGCATCTTCCTT+GGG 0.462060 2.1:-74607093 None:intergenic
CATTCAATGCTTTATCAAAA+GGG 0.462254 2.1:+74605729 MS.gene01112:CDS
CAGGGCTCAGACAAAGGAAT+AGG 0.465192 2.1:-74605774 None:intergenic
AATTGTTCATGCCATCTTGG+AGG 0.466985 2.1:-74607334 None:intergenic
TGACGGCAAGATGAGACCTT+TGG 0.479423 2.1:+74607297 MS.gene01112:CDS
TCTGCCCATATATGTCCTCC+GGG 0.481297 2.1:+74606959 MS.gene01112:CDS
TGAATTAGCTCTGGCTGTCC+GGG 0.484556 2.1:-74607160 None:intergenic
TAGAGGAGAGTGAGAATACT+TGG 0.484573 2.1:+74605447 MS.gene01112:CDS
CTATTCCTTTGTCTGAGCCC+TGG 0.486417 2.1:+74605775 MS.gene01112:CDS
ATAATGCAATGCATCTTCCT+TGG 0.491165 2.1:-74607094 None:intergenic
AAACTATTCACAAGCCAACT+TGG 0.491824 2.1:-74607596 None:intergenic
TTATCATGTTCGGGTGGTGC+TGG 0.495403 2.1:-74607466 None:intergenic
TTAAGCATATTGCAAAAGCT+TGG 0.496726 2.1:-74607561 None:intergenic
GTTCCCTGGAGGACTCGTTC+CGG 0.496788 2.1:+74607185 MS.gene01112:CDS
ATGATTCCGTCTCGTGGCCC+TGG 0.498403 2.1:-74605709 None:intergenic
TCCTCTCTGCTAAGAGGACC+AGG 0.507703 2.1:+74606337 MS.gene01112:CDS
TTAGTTTGCCGCAGAACTCT+GGG 0.509843 2.1:-74605547 None:intergenic
AAAGTCTCATGGTTCTGTAC+AGG 0.510601 2.1:+74605340 MS.gene01112:CDS
TGTAATTCAAAATAGTTGCT+GGG 0.513418 2.1:+74605382 MS.gene01112:CDS
AGAGCTAATTCACCGTTCCC+TGG 0.515311 2.1:+74607171 MS.gene01112:CDS
ACCAAATTCAGCACTTGATG+CGG 0.517901 2.1:+74607126 MS.gene01112:CDS
ACTTCGAAAATGAGATGCTC+CGG 0.519823 2.1:-74607204 None:intergenic
CGCCCCAGCAGGTGGTTGGG+TGG 0.525317 2.1:-74607428 None:intergenic
AGGGAACGGTGAATTAGCTC+TGG 0.526856 2.1:-74607169 None:intergenic
TAGCTCTGGCTGTCCGGGAA+CGG 0.532391 2.1:-74607155 None:intergenic
GAGACTTTCCTCTATGATGA+TGG 0.534079 2.1:-74605325 None:intergenic
TGCACATATTCCTCCAAGAC+TGG 0.534318 2.1:-74605508 None:intergenic
AATTCCTCCTCTCTGCTAAG+AGG 0.537777 2.1:+74606331 MS.gene01112:CDS
GTTTACCAGGGCTCAGACAA+AGG 0.542225 2.1:-74605780 None:intergenic
TTCCACCCAACCACCTGCTG+GGG 0.546085 2.1:+74607426 MS.gene01112:CDS
GGTGCTGGTTGAGATGGTGT+CGG 0.550204 2.1:-74607451 None:intergenic
CCAAATTCAGCACTTGATGC+GGG 0.550546 2.1:+74607127 MS.gene01112:CDS
GGTGTCGGCGCCCCAGCAGG+TGG 0.552680 2.1:-74607436 None:intergenic
CAATCGAGTTCAGCAGTGCA+CGG 0.554602 2.1:+74607239 MS.gene01112:CDS
AAGCAGCTTTGACACCAAGT+TGG 0.560190 2.1:+74607582 MS.gene01112:CDS
CGGCTCGTCTTCCCAGTCCC+CGG 0.560397 2.1:-74606977 None:intergenic
TCGGGTGGTGCTGGTTGAGA+TGG 0.560858 2.1:-74607457 None:intergenic
AGTCCCCGGAGGACATATAT+GGG 0.561123 2.1:-74606963 None:intergenic
AGATCAAGTAATACTTACAT+TGG 0.570296 2.1:+74606411 MS.gene01112:CDS
ACATATGAACTAAATGTGCT+CGG 0.570299 2.1:+74607061 MS.gene01112:CDS
CTAAATGTGCTCGGAACTCG+AGG 0.572448 2.1:+74607070 MS.gene01112:CDS
CTAACCTGTTTCAGGGACAC+AGG 0.573911 2.1:-74605575 None:intergenic
TCTGCAACTAACCTGTTTCA+GGG 0.575798 2.1:-74605582 None:intergenic
TGGGAGTTACAACATAGCTC+AGG 0.584581 2.1:+74607036 MS.gene01112:CDS
CTTACTGGCCATCATCATAG+AGG 0.586395 2.1:+74605317 MS.gene01112:CDS
GACATCAAATAGTAATTCTG+GGG 0.586583 2.1:-74605414 None:intergenic
GGCATGAACAATTACAGTGC+TGG 0.588906 2.1:+74607344 MS.gene01112:CDS
TGATGTCATTCGGCGACTAG+AGG 0.590244 2.1:+74605430 MS.gene01112:CDS
GGAACGAGTCCTCCAGGGAA+CGG 0.592037 2.1:-74607183 None:intergenic
CATCATAGAGGAAAGTCTCA+TGG 0.596493 2.1:+74605329 MS.gene01112:CDS
TGAAATAACATACTCAGTGT+AGG 0.597863 2.1:-74606367 None:intergenic
TAAAGGATATCGATAATCCA+TGG 0.599763 2.1:-74607530 None:intergenic
GCTATGTTGTAACTCCCAGA+TGG 0.603839 2.1:-74607031 None:intergenic
CATTGAATGATTCCGTCTCG+TGG 0.607917 2.1:-74605715 None:intergenic
CTATGTTGTAACTCCCAGAT+GGG 0.609999 2.1:-74607030 None:intergenic
TGCTCGGAACTCGAGGCCCA+AGG 0.612968 2.1:+74607077 MS.gene01112:CDS
TATCGCCAAAGGTCCCATCT+GGG 0.614613 2.1:+74607017 MS.gene01112:CDS
TTTCAGCCAGGGCCACGAGA+CGG 0.619741 2.1:+74605703 MS.gene01112:intron
GAACGTCACGAACTATGCTA+CGG 0.624312 2.1:-74605250 None:intergenic
TAACCTGTTTCAGGGACACA+GGG 0.626828 2.1:-74605574 None:intergenic
TCCTGGTCCTCTTAGCAGAG+AGG 0.627316 2.1:-74606338 None:intergenic
CATATATGGGCAGAAGTGCA+GGG 0.629040 2.1:-74606950 None:intergenic
TCCTGTAATGAGGATGATGA+CGG 0.629322 2.1:+74607280 MS.gene01112:CDS
GTCTTGGAGGAATATGTGCA+AGG 0.636885 2.1:+74605511 MS.gene01112:CDS
TGGGAAAGATATGTTCACCA+TGG 0.642687 2.1:+74607513 MS.gene01112:CDS
GTTAAGAGCCCAGAGTTCTG+CGG 0.649777 2.1:+74605539 MS.gene01112:CDS
TGGTCCTCTTAGCAGAGAGG+AGG 0.654986 2.1:-74606335 None:intergenic
GCTCCGGAACGAGTCCTCCA+GGG 0.659722 2.1:-74607188 None:intergenic
ATACTTACATTGGAAAACTG+AGG 0.671640 2.1:+74606421 MS.gene01112:CDS
ACATCAAATAGTAATTCTGG+GGG 0.673779 2.1:-74605413 None:intergenic
AATTCAGCACTTGATGCGGG+CGG 0.674604 2.1:+74607130 MS.gene01112:CDS
ATAATCTTATCATGTTCGGG+TGG 0.690693 2.1:-74607472 None:intergenic
CTCGTCTTCCCAGTCCCCGG+AGG 0.692752 2.1:-74606974 None:intergenic
GCTAATTCACCGTTCCCTGG+AGG 0.694493 2.1:+74607174 MS.gene01112:CDS
CTGCCCATATATGTCCTCCG+GGG 0.741928 2.1:+74606960 MS.gene01112:CDS
ATATGGGCAGAAGTGCAGGG+AGG 0.788147 2.1:-74606947 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAAATAAAATTGAAGC+AGG - chr2.1:74606279-74606298 None:intergenic 20.0%
!! AATTACTATTTGATGTCATT+CGG + chr2.1:74605420-74605439 MS.gene01112:CDS 20.0%
!! CATAAGTGGAAAATTATTTA+TGG - chr2.1:74606761-74606780 None:intergenic 20.0%
!! TGAAATAACAACAATAGTTA+AGG + chr2.1:74605647-74605666 MS.gene01112:intron 20.0%
!! TTTCAAAATTTAAGCAGTTT+AGG + chr2.1:74605896-74605915 MS.gene01112:intron 20.0%
!!! ATACCTTTTTAGAAAATCTA+CGG - chr2.1:74607000-74607019 None:intergenic 20.0%
!!! CTATGTTAAAAACTTCTTTT+CGG + chr2.1:74606484-74606503 MS.gene01112:intron 20.0%
! AAATATTCATGGAATGACAA+CGG - chr2.1:74606076-74606095 None:intergenic 25.0%
! AGAATAATCTTATCATGTTC+GGG - chr2.1:74607478-74607497 None:intergenic 25.0%
! AGATCAAGTAATACTTACAT+TGG + chr2.1:74606411-74606430 MS.gene01112:CDS 25.0%
! ATGACATCAAATAGTAATTC+TGG - chr2.1:74605419-74605438 None:intergenic 25.0%
! CAAAGGGAAATAAATATTCA+TGG - chr2.1:74606087-74606106 None:intergenic 25.0%
! CAGAATAATCTTATCATGTT+CGG - chr2.1:74607479-74607498 None:intergenic 25.0%
! CATTCAATGCTTTATCAAAA+GGG + chr2.1:74605729-74605748 MS.gene01112:CDS 25.0%
! GATAAGATTATTCTGCAATT+CGG + chr2.1:74607484-74607503 MS.gene01112:CDS 25.0%
! TCATTCAATGCTTTATCAAA+AGG + chr2.1:74605728-74605747 MS.gene01112:CDS 25.0%
! TGACATCAAATAGTAATTCT+GGG - chr2.1:74605418-74605437 None:intergenic 25.0%
! TGTAATTCAAAATAGTTGCT+GGG + chr2.1:74605382-74605401 MS.gene01112:CDS 25.0%
! TGTGTTGTAACTGTTATTAT+CGG + chr2.1:74606706-74606725 MS.gene01112:intron 25.0%
! TTGTAATTCAAAATAGTTGC+TGG + chr2.1:74605381-74605400 MS.gene01112:CDS 25.0%
!! TCTTTTATCGTCATATGTTT+AGG + chr2.1:74606657-74606676 MS.gene01112:intron 25.0%
!! TGATTCTTCTGTTTTACTTT+TGG - chr2.1:74606587-74606606 None:intergenic 25.0%
!!! AACATCTCATTTTTATGCAT+CGG - chr2.1:74606046-74606065 None:intergenic 25.0%
!!! CAACTATTTTGAATTACAAG+AGG - chr2.1:74605380-74605399 None:intergenic 25.0%
!!! GATTCTTCTGTTTTACTTTT+GGG - chr2.1:74606586-74606605 None:intergenic 25.0%
!!! GTTTAATTTGAGATCTACTT+TGG + chr2.1:74605993-74606012 MS.gene01112:intron 25.0%
!!! TTAAAATGCGTTTTGATAGT+CGG - chr2.1:74606561-74606580 None:intergenic 25.0%
AACAGAAAAAACTCAGCAAA+TGG - chr2.1:74606824-74606843 None:intergenic 30.0%
AAGTTGCAATGAATTTCAGT+TGG + chr2.1:74606618-74606637 MS.gene01112:intron 30.0%
ACATATGAACTAAATGTGCT+CGG + chr2.1:74607061-74607080 MS.gene01112:CDS 30.0%
ACATCAAATAGTAATTCTGG+GGG - chr2.1:74605416-74605435 None:intergenic 30.0%
ATACTTACATTGGAAAACTG+AGG + chr2.1:74606421-74606440 MS.gene01112:CDS 30.0%
CTGTTTGATATCCATTATGT+CGG + chr2.1:74606134-74606153 MS.gene01112:intron 30.0%
GAAGATATGAATAGCAAACA+AGG - chr2.1:74606797-74606816 None:intergenic 30.0%
GACATCAAATAGTAATTCTG+GGG - chr2.1:74605417-74605436 None:intergenic 30.0%
TAAAACCATATATACCCACT+AGG - chr2.1:74605828-74605847 None:intergenic 30.0%
TAAAGGATATCGATAATCCA+TGG - chr2.1:74607533-74607552 None:intergenic 30.0%
TGAAATAACATACTCAGTGT+AGG - chr2.1:74606370-74606389 None:intergenic 30.0%
TTAACATAGTAGATGAGAGA+AGG - chr2.1:74606473-74606492 None:intergenic 30.0%
TTAAGCATATTGCAAAAGCT+TGG - chr2.1:74607564-74607583 None:intergenic 30.0%
!! AGTTTTGATGTCAAACTTAC+TGG + chr2.1:74605302-74605321 MS.gene01112:CDS 30.0%
!! CACTGAGTATGTTATTTCAA+TGG + chr2.1:74606371-74606390 MS.gene01112:CDS 30.0%
!!! ACTTCTAAAGTTTTGATGCT+AGG + chr2.1:74606213-74606232 MS.gene01112:intron 30.0%
!!! CTTCTAAAGTTTTGATGCTA+GGG + chr2.1:74606214-74606233 MS.gene01112:intron 30.0%
AAAGCTTGGAATGCAGATAA+AGG - chr2.1:74607550-74607569 None:intergenic 35.0%
ACTCCTAGAATCAATCACAA+AGG - chr2.1:74606104-74606123 None:intergenic 35.0%
ATAATCTTATCATGTTCGGG+TGG - chr2.1:74607475-74607494 None:intergenic 35.0%
ATAATGCAATGCATCTTCCT+TGG - chr2.1:74607097-74607116 None:intergenic 35.0%
CGGAGGTAATAAATAGTCTA+GGG + chr2.1:74606726-74606745 MS.gene01112:intron 35.0%
CTCCTAGAATCAATCACAAA+GGG - chr2.1:74606103-74606122 None:intergenic 35.0%
GAATATCAGCATCACATAAG+TGG - chr2.1:74606775-74606794 None:intergenic 35.0%
GTTGTAACTGTTATTATCGG+AGG + chr2.1:74606709-74606728 MS.gene01112:intron 35.0%
TAATGCAATGCATCTTCCTT+GGG - chr2.1:74607096-74607115 None:intergenic 35.0%
TACACCAGATCAAACTTTCT+TGG + chr2.1:74606896-74606915 MS.gene01112:intron 35.0%
TATATACCCACTAGGTAACT+AGG - chr2.1:74605820-74605839 None:intergenic 35.0%
TCGGAGGTAATAAATAGTCT+AGG + chr2.1:74606725-74606744 MS.gene01112:intron 35.0%
TGTAATTGTTCATGCCATCT+TGG - chr2.1:74607340-74607359 None:intergenic 35.0%
TGTTCCAAGAAAGTTTGATC+TGG - chr2.1:74606903-74606922 None:intergenic 35.0%
TTGATATCCATTATGTCGGA+GGG + chr2.1:74606138-74606157 MS.gene01112:intron 35.0%
TTTGATATCCATTATGTCGG+AGG + chr2.1:74606137-74606156 MS.gene01112:intron 35.0%
! AGTTACCTAGTGGGTATATA+TGG + chr2.1:74605820-74605839 MS.gene01112:intron 35.0%
! GTTTTATGTTCAGATGCTTC+TGG + chr2.1:74605842-74605861 MS.gene01112:intron 35.0%
! TTCCCTTTGTGATTGATTCT+AGG + chr2.1:74606098-74606117 MS.gene01112:intron 35.0%
!! GAGCCGTAGATTTTCTAAAA+AGG + chr2.1:74606994-74607013 MS.gene01112:CDS 35.0%
!! GCAGCTTTGTTAGTTGAAAA+TGG + chr2.1:74606306-74606325 MS.gene01112:intron 35.0%
!! TTCTAAAAAGGTATCGCCAA+AGG + chr2.1:74607006-74607025 MS.gene01112:CDS 35.0%
!!! ATAGTTTTGGGAGCTTATCT+AGG + chr2.1:74605276-74605295 MS.gene01112:CDS 35.0%
AATTGTTCATGCCATCTTGG+AGG - chr2.1:74607337-74607356 None:intergenic 40.0%
ACCAAATTCAGCACTTGATG+CGG + chr2.1:74607126-74607145 MS.gene01112:CDS 40.0%
ACTTCGAAAATGAGATGCTC+CGG - chr2.1:74607207-74607226 None:intergenic 40.0%
ATCTGCAACTAACCTGTTTC+AGG - chr2.1:74605586-74605605 None:intergenic 40.0%
ATTCTGCAATTCGGCAAAGT+TGG + chr2.1:74607493-74607512 MS.gene01112:CDS 40.0%
CATCATAGAGGAAAGTCTCA+TGG + chr2.1:74605329-74605348 MS.gene01112:CDS 40.0%
CTATGTTGTAACTCCCAGAT+GGG - chr2.1:74607033-74607052 None:intergenic 40.0%
GAGACTTTCCTCTATGATGA+TGG - chr2.1:74605328-74605347 None:intergenic 40.0%
TAGAGGAGAGTGAGAATACT+TGG + chr2.1:74605447-74605466 MS.gene01112:CDS 40.0%
TCCTGTAATGAGGATGATGA+CGG + chr2.1:74607280-74607299 MS.gene01112:CDS 40.0%
TCTGCAACTAACCTGTTTCA+GGG - chr2.1:74605585-74605604 None:intergenic 40.0%
TGAAAAACAAGCCTCCAAGA+TGG + chr2.1:74607323-74607342 MS.gene01112:CDS 40.0%
TGGGAAAGATATGTTCACCA+TGG + chr2.1:74607513-74607532 MS.gene01112:CDS 40.0%
TTACTCGTCCCCGAAAATTA+AGG - chr2.1:74607372-74607391 None:intergenic 40.0%
TTCTGCAATTCGGCAAAGTT+GGG + chr2.1:74607494-74607513 MS.gene01112:CDS 40.0%
! AAAGTCTCATGGTTCTGTAC+AGG + chr2.1:74605340-74605359 MS.gene01112:CDS 40.0%
! GTGACGTTCGTGATAGTTTT+GGG + chr2.1:74605264-74605283 MS.gene01112:CDS 40.0%
! GTGATTGATTCTAGGAGTCT+TGG + chr2.1:74606106-74606125 MS.gene01112:intron 40.0%
!! CGTTTTGATAGTCGGAAACT+CGG - chr2.1:74606553-74606572 None:intergenic 40.0%
!! GGCTTGTTTTTCAGAACCAA+AGG - chr2.1:74607316-74607335 None:intergenic 40.0%
!! TAAGCGAAGAGTGTTTACCA+GGG - chr2.1:74605795-74605814 None:intergenic 40.0%
!! TTCGTTTCACTTTTCAGCCA+GGG + chr2.1:74605692-74605711 MS.gene01112:intron 40.0%
!!! AGTGCTGGTGCCTTAATTTT+CGG + chr2.1:74607359-74607378 MS.gene01112:CDS 40.0%
AATTCCTCCTCTCTGCTAAG+AGG + chr2.1:74606331-74606350 MS.gene01112:CDS 45.0%
CATTGAATGATTCCGTCTCG+TGG - chr2.1:74605718-74605737 None:intergenic 45.0%
CCAAATTCAGCACTTGATGC+GGG + chr2.1:74607127-74607146 MS.gene01112:CDS 45.0%
CTTACGCCTAGTTACCTAGT+GGG + chr2.1:74605811-74605830 MS.gene01112:intron 45.0%
CTTACTGGCCATCATCATAG+AGG + chr2.1:74605317-74605336 MS.gene01112:CDS 45.0%
GAACGTCACGAACTATGCTA+CGG - chr2.1:74605253-74605272 None:intergenic 45.0%
GCAACTACATGCTTCCTTGA+AGG - chr2.1:74605473-74605492 None:intergenic 45.0%
GCTATGTTGTAACTCCCAGA+TGG - chr2.1:74607034-74607053 None:intergenic 45.0%
GGCATGAACAATTACAGTGC+TGG + chr2.1:74607344-74607363 MS.gene01112:CDS 45.0%
GTCTTGGAGGAATATGTGCA+AGG + chr2.1:74605511-74605530 MS.gene01112:CDS 45.0%
TAACCTGTTTCAGGGACACA+GGG - chr2.1:74605577-74605596 None:intergenic 45.0%
TGCACATATTCCTCCAAGAC+TGG - chr2.1:74605511-74605530 None:intergenic 45.0%
TGGGAGTTACAACATAGCTC+AGG + chr2.1:74607036-74607055 MS.gene01112:CDS 45.0%
TTCTGCCCATATATGTCCTC+CGG + chr2.1:74606958-74606977 MS.gene01112:CDS 45.0%
! AAGCAGCTTTGACACCAAGT+TGG + chr2.1:74607582-74607601 MS.gene01112:CDS 45.0%
! ACATATATGGGCAGAAGTGC+AGG - chr2.1:74606954-74606973 None:intergenic 45.0%
! CATATATGGGCAGAAGTGCA+GGG - chr2.1:74606953-74606972 None:intergenic 45.0%
! CGTGACGTTCGTGATAGTTT+TGG + chr2.1:74605263-74605282 MS.gene01112:CDS 45.0%
! GTGAGAATACTTGGCCTTCA+AGG + chr2.1:74605456-74605475 MS.gene01112:CDS 45.0%
! TGCTGGTGCCTTAATTTTCG+GGG + chr2.1:74607361-74607380 MS.gene01112:CDS 45.0%
!! GTAAGCGAAGAGTGTTTACC+AGG - chr2.1:74605796-74605815 None:intergenic 45.0%
!! GTGCTGGTGCCTTAATTTTC+GGG + chr2.1:74607360-74607379 MS.gene01112:CDS 45.0%
!! GTTCGTTTCACTTTTCAGCC+AGG + chr2.1:74605691-74605710 MS.gene01112:intron 45.0%
!! TTAGTTTGCCGCAGAACTCT+GGG - chr2.1:74605550-74605569 None:intergenic 45.0%
!!! AGTGCACGGTTTTCTGAGAT+CGG + chr2.1:74607253-74607272 MS.gene01112:CDS 45.0%
AATTCAGCACTTGATGCGGG+CGG + chr2.1:74607130-74607149 MS.gene01112:CDS 50.0%
AGAGCTAATTCACCGTTCCC+TGG + chr2.1:74607171-74607190 MS.gene01112:CDS 50.0%
AGGGAACGGTGAATTAGCTC+TGG - chr2.1:74607172-74607191 None:intergenic 50.0%
AGTCCCCGGAGGACATATAT+GGG - chr2.1:74606966-74606985 None:intergenic 50.0%
ATATATGTCCTCCGGGGACT+GGG + chr2.1:74606966-74606985 MS.gene01112:CDS 50.0%
ATCCATTATGTCGGAGGGCA+AGG + chr2.1:74606143-74606162 MS.gene01112:intron 50.0%
ATTCCCTGTGTCCCTGAAAC+AGG + chr2.1:74605571-74605590 MS.gene01112:CDS 50.0%
CAATCGAGTTCAGCAGTGCA+CGG + chr2.1:74607239-74607258 MS.gene01112:CDS 50.0%
CAGGGCTCAGACAAAGGAAT+AGG - chr2.1:74605777-74605796 None:intergenic 50.0%
CTAAATGTGCTCGGAACTCG+AGG + chr2.1:74607070-74607089 MS.gene01112:CDS 50.0%
CTAACCTGTTTCAGGGACAC+AGG - chr2.1:74605578-74605597 None:intergenic 50.0%
CTATTCCTTTGTCTGAGCCC+TGG + chr2.1:74605775-74605794 MS.gene01112:CDS 50.0%
CTCAGTGTAGGTTGTTCTCC+TGG - chr2.1:74606358-74606377 None:intergenic 50.0%
CTTCTGTTTGCCAGTCTTGG+AGG + chr2.1:74605498-74605517 MS.gene01112:CDS 50.0%
GCCGTCATCATCCTCATTAC+AGG - chr2.1:74607284-74607303 None:intergenic 50.0%
GCTTACGCCTAGTTACCTAG+TGG + chr2.1:74605810-74605829 MS.gene01112:intron 50.0%
GTGCTTCTGTTTGCCAGTCT+TGG + chr2.1:74605495-74605514 MS.gene01112:CDS 50.0%
GTTAAGAGCCCAGAGTTCTG+CGG + chr2.1:74605539-74605558 MS.gene01112:CDS 50.0%
GTTTACCAGGGCTCAGACAA+AGG - chr2.1:74605783-74605802 None:intergenic 50.0%
TAACTCCCAGATGGGACCTT+TGG - chr2.1:74607025-74607044 None:intergenic 50.0%
TATCGCCAAAGGTCCCATCT+GGG + chr2.1:74607017-74607036 MS.gene01112:CDS 50.0%
TCTGCCCATATATGTCCTCC+GGG + chr2.1:74606959-74606978 MS.gene01112:CDS 50.0%
TGACGGCAAGATGAGACCTT+TGG + chr2.1:74607297-74607316 MS.gene01112:CDS 50.0%
TGATGTCATTCGGCGACTAG+AGG + chr2.1:74605430-74605449 MS.gene01112:CDS 50.0%
TTATCATGTTCGGGTGGTGC+TGG - chr2.1:74607469-74607488 None:intergenic 50.0%
!! CCCGCATCAAGTGCTGAATT+TGG - chr2.1:74607130-74607149 None:intergenic 50.0%
!! GTGAATTAGCTCTGGCTGTC+CGG - chr2.1:74607164-74607183 None:intergenic 50.0%
!! GTTAGTTTGCCGCAGAACTC+TGG - chr2.1:74605551-74605570 None:intergenic 50.0%
!! TGAATTAGCTCTGGCTGTCC+GGG - chr2.1:74607163-74607182 None:intergenic 50.0%
!!! GTGCACGGTTTTCTGAGATC+GGG + chr2.1:74607254-74607273 MS.gene01112:CDS 50.0%
!!! TGCACGGTTTTCTGAGATCG+GGG + chr2.1:74607255-74607274 MS.gene01112:CDS 50.0%
AGCCTTGCCCTCCGACATAA+TGG - chr2.1:74606148-74606167 None:intergenic 55.0%
CAGTCCCCGGAGGACATATA+TGG - chr2.1:74606967-74606986 None:intergenic 55.0%
CATATATGTCCTCCGGGGAC+TGG + chr2.1:74606965-74606984 MS.gene01112:CDS 55.0%
CTGCCCATATATGTCCTCCG+GGG + chr2.1:74606960-74606979 MS.gene01112:CDS 55.0%
GATCGGGGGATCCTGTAATG+AGG + chr2.1:74607270-74607289 MS.gene01112:CDS 55.0%
GCTAATTCACCGTTCCCTGG+AGG + chr2.1:74607174-74607193 MS.gene01112:CDS 55.0%
GTATCGCCAAAGGTCCCATC+TGG + chr2.1:74607016-74607035 MS.gene01112:CDS 55.0%
TCCTCTCTGCTAAGAGGACC+AGG + chr2.1:74606337-74606356 MS.gene01112:CDS 55.0%
TCCTGGTCCTCTTAGCAGAG+AGG - chr2.1:74606341-74606360 None:intergenic 55.0%
TGGTCCTCTTAGCAGAGAGG+AGG - chr2.1:74606338-74606357 None:intergenic 55.0%
TGTCGGAGGGCAAGGCTTAA+TGG + chr2.1:74606151-74606170 MS.gene01112:intron 55.0%
! ATATGGGCAGAAGTGCAGGG+AGG - chr2.1:74606950-74606969 None:intergenic 55.0%
!! GGTGCTGGTTGAGATGGTGT+CGG - chr2.1:74607454-74607473 None:intergenic 55.0%
!!! GCACGGTTTTCTGAGATCGG+GGG + chr2.1:74607256-74607275 MS.gene01112:CDS 55.0%
ATGATTCCGTCTCGTGGCCC+TGG - chr2.1:74605712-74605731 None:intergenic 60.0%
CTTCCACCCAACCACCTGCT+GGG + chr2.1:74607425-74607444 MS.gene01112:CDS 60.0%
GGAACGAGTCCTCCAGGGAA+CGG - chr2.1:74607186-74607205 None:intergenic 60.0%
GTTCCCTGGAGGACTCGTTC+CGG + chr2.1:74607185-74607204 MS.gene01112:CDS 60.0%
TTCCACCCAACCACCTGCTG+GGG + chr2.1:74607426-74607445 MS.gene01112:CDS 60.0%
TTTCAGCCAGGGCCACGAGA+CGG + chr2.1:74605703-74605722 MS.gene01112:intron 60.0%
!! TAGCTCTGGCTGTCCGGGAA+CGG - chr2.1:74607158-74607177 None:intergenic 60.0%
!! TCGGGTGGTGCTGGTTGAGA+TGG - chr2.1:74607460-74607479 None:intergenic 60.0%
GCTCCGGAACGAGTCCTCCA+GGG - chr2.1:74607191-74607210 None:intergenic 65.0%
GCTTCCACCCAACCACCTGC+TGG + chr2.1:74607424-74607443 MS.gene01112:CDS 65.0%
TGCTCCGGAACGAGTCCTCC+AGG - chr2.1:74607192-74607211 None:intergenic 65.0%
TGCTCGGAACTCGAGGCCCA+AGG + chr2.1:74607077-74607096 MS.gene01112:CDS 65.0%
CGGCTCGTCTTCCCAGTCCC+CGG - chr2.1:74606980-74606999 None:intergenic 70.0%
CTCGTCTTCCCAGTCCCCGG+AGG - chr2.1:74606977-74606996 None:intergenic 70.0%
CGCCCCAGCAGGTGGTTGGG+TGG - chr2.1:74607431-74607450 None:intergenic 75.0%
CGGCGCCCCAGCAGGTGGTT+GGG - chr2.1:74607434-74607453 None:intergenic 75.0%
GATGCGGGCGGCTCCGTTCC+CGG + chr2.1:74607142-74607161 MS.gene01112:CDS 75.0%
TCGGCGCCCCAGCAGGTGGT+TGG - chr2.1:74607435-74607454 None:intergenic 75.0%
!! GATGGTGTCGGCGCCCCAGC+AGG - chr2.1:74607442-74607461 None:intergenic 75.0%
!!! GGTGTCGGCGCCCCAGCAGG+TGG - chr2.1:74607439-74607458 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 74605242 74607615 74605242 ID=MS.gene01112
chr2.1 mRNA 74605242 74607615 74605242 ID=MS.gene01112.t1;Parent=MS.gene01112
chr2.1 exon 74605242 74605592 74605242 ID=MS.gene01112.t1.exon1;Parent=MS.gene01112.t1
chr2.1 CDS 74605242 74605592 74605242 ID=cds.MS.gene01112.t1;Parent=MS.gene01112.t1
chr2.1 exon 74605709 74605796 74605709 ID=MS.gene01112.t1.exon2;Parent=MS.gene01112.t1
chr2.1 CDS 74605709 74605796 74605709 ID=cds.MS.gene01112.t1;Parent=MS.gene01112.t1
chr2.1 exon 74606310 74606442 74606310 ID=MS.gene01112.t1.exon3;Parent=MS.gene01112.t1
chr2.1 CDS 74606310 74606442 74606310 ID=cds.MS.gene01112.t1;Parent=MS.gene01112.t1
chr2.1 exon 74606904 74607615 74606904 ID=MS.gene01112.t1.exon4;Parent=MS.gene01112.t1
chr2.1 CDS 74606904 74607615 74606904 ID=cds.MS.gene01112.t1;Parent=MS.gene01112.t1
Gene Sequence

>MS.gene01112

ATGTCTTTCCGTAGCATAGTTCGTGACGTTCGTGATAGTTTTGGGAGCTTATCTAGGCGTAGTTTTGATGTCAAACTTACTGGCCATCATCATAGAGGAAAGTCTCATGGTTCTGTACAGGATTTGCATGATCAGCCTCTTGTAATTCAAAATAGTTGCTGGGCGAACTTACCCCCAGAATTACTATTTGATGTCATTCGGCGACTAGAGGAGAGTGAGAATACTTGGCCTTCAAGGAAGCATGTAGTTGCATGTGCTTCTGTTTGCCAGTCTTGGAGGAATATGTGCAAGGACATTGTTAAGAGCCCAGAGTTCTGCGGCAAACTAACATTCCCTGTGTCCCTGAAACAGGTTAGTTGCAGATTATCTCACTTTTTATTTATTAAGAATCAATTACATTTATGTTGAAATAACAACAATAGTTAAGGTAGCAACATGCTGACTTTGTCGTTCGTTTCACTTTTCAGCCAGGGCCACGAGACGGAATCATTCAATGCTTTATCAAAAGGGATAAATCTAACTTAACATATCACCTATTCCTTTGTCTGAGCCCTGGTAAACACTCTTCGCTTACGCCTAGTTACCTAGTGGGTATATATGGTTTTATGTTCAGATGCTTCTGGTGTGATTGTGTAAATTTGTTCTTATGTAATGTTTCAAAATTTAAGCAGTTTAGGTTTTTTAAGTTACTGCTATATGAATATTTGTGCATGTGTTGTTTGTCGCTGCACGTGAAGATGTATGCATCGATGTTTAATTTGAGATCTACTTTGGATGACACTTGTAATAGTGTGACTTGTGCCGATGCATAAAAATGAGATGTTTCTATAACCGTTGTCATTCCATGAATATTTATTTCCCTTTGTGATTGATTCTAGGAGTCTTGGATATTCTGTTTGATATCCATTATGTCGGAGGGCAAGGCTTAATGGTCTATTTTGTCGTTTTATATTAATTATTTTAGCAACATTACTTCTAAAGTTTTGATGCTAGGGAAGTTGTCTACAGCATCTTGAACTCATTTTAGTTTTGTTCCTGCTTCAATTTTATTTACTTTGACTTTTGCAGCTTTGTTAGTTGAAAATGGAAAATTCCTCCTCTCTGCTAAGAGGACCAGGAGAACAACCTACACTGAGTATGTTATTTCAATGGATGCTGACAACATCTCTAGATCAAGTAATACTTACATTGGAAAACTGAGGTAAATTTACTACTTGCAAATGTTGATCCTTCTCTCATCTACTATGTTAAAAACTTCTTTTCGGTGTCTATAAAAATATACTGTTAAAACTACTATGAATTATGATTCCGAGTTTCCGACTATCAAAACGCATTTTAACTCCCAAAAGTAAAACAGAAGAATCAATGATCTCGCAAAGTTGCAATGAATTTCAGTTGGTTTATGATTAAGTACTTCTTTTATCGTCATATGTTTAGGTGCACGAAATTATAATTATACAAAAATGTGTTGTAACTGTTATTATCGGAGGTAATAAATAGTCTAGGGTTCTTTATTCCATAAATAATTTTCCACTTATGTGATGCTGATATTCCTTGTTTGCTATTCATATCTTCAAGACCATTTGCTGAGTTTTTTCTGTTCTATTTTATATCTATGTCTTCTCACTTGCAAGATTATTTGTTATTATTTTGATACACCAGATCAAACTTTCTTGGAACAAAGTTCATCATATATGATACACAGCCTCCCTGCACTTCTGCCCATATATGTCCTCCGGGGACTGGGAAGACGAGCCGTAGATTTTCTAAAAAGGTATCGCCAAAGGTCCCATCTGGGAGTTACAACATAGCTCAGGTAACATATGAACTAAATGTGCTCGGAACTCGAGGCCCAAGGAAGATGCATTGCATTATGCATTCAATACCAAATTCAGCACTTGATGCGGGCGGCTCCGTTCCCGGACAGCCAGAGCTAATTCACCGTTCCCTGGAGGACTCGTTCCGGAGCATCTCATTTTCGAAGTCTCTAGATCATTCAATCGAGTTCAGCAGTGCACGGTTTTCTGAGATCGGGGGATCCTGTAATGAGGATGATGACGGCAAGATGAGACCTTTGGTTCTGAAAAACAAGCCTCCAAGATGGCATGAACAATTACAGTGCTGGTGCCTTAATTTTCGGGGACGAGTAACTGTTGCATCTGTTAAAAACTTTCAGCTGATTGCTTCCACCCAACCACCTGCTGGGGCGCCGACACCATCTCAACCAGCACCACCCGAACATGATAAGATTATTCTGCAATTCGGCAAAGTTGGGAAAGATATGTTCACCATGGATTATCGATATCCTTTATCTGCATTCCAAGCTTTTGCAATATGCTTAAGCAGCTTTGACACCAAGTTGGCTTGTGAATAG
Protein sequence

>MS.gene01112.t1

MSFRSIVRDVRDSFGSLSRRSFDVKLTGHHHRGKSHGSVQDLHDQPLVIQNSCWANLPPELLFDVIRRLEESENTWPSRKHVVACASVCQSWRNMCKDIVKSPEFCGKLTFPVSLKQPGPRDGIIQCFIKRDKSNLTYHLFLCLSPALLVENGKFLLSAKRTRRTTYTEYVISMDADNISRSSNTYIGKLRSNFLGTKFIIYDTQPPCTSAHICPPGTGKTSRRFSKKVSPKVPSGSYNIAQVTYELNVLGTRGPRKMHCIMHSIPNSALDAGGSVPGQPELIHRSLEDSFRSISFSKSLDHSIEFSSARFSEIGGSCNEDDDGKMRPLVLKNKPPRWHEQLQCWCLNFRGRVTVASVKNFQLIASTQPPAGAPTPSQPAPPEHDKIILQFGKVGKDMFTMDYRYPLSAFQAFAICLSSFDTKLACE