Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011346.t1 | KEH15972.1 | 48.5 | 99 | 9 | 1 | 6 | 62 | 57 | 155 | 6.80E-10 | 73.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011346.t1 | Q6S4P4 | 72.0 | 50 | 14 | 0 | 33 | 82 | 134 | 183 | 6.4e-09 | 61.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011346.t1 | A0A072TF27 | 48.5 | 99 | 9 | 1 | 6 | 62 | 57 | 155 | 4.9e-10 | 73.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene011346.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011346.t1 | MTR_0405s0060 | 47.525 | 101 | 11 | 1 | 6 | 64 | 57 | 157 | 2.29e-19 | 80.1 |
| MS.gene011346.t1 | MTR_4g072090 | 70.492 | 61 | 17 | 1 | 23 | 82 | 351 | 411 | 1.26e-17 | 77.0 |
| MS.gene011346.t1 | MTR_4g072090 | 70.492 | 61 | 17 | 1 | 23 | 82 | 351 | 411 | 1.57e-17 | 77.0 |
| MS.gene011346.t1 | MTR_2g099050 | 72.000 | 50 | 14 | 0 | 33 | 82 | 223 | 272 | 8.86e-16 | 72.0 |
| MS.gene011346.t1 | MTR_3g467120 | 60.000 | 70 | 22 | 1 | 19 | 82 | 159 | 228 | 3.32e-15 | 70.1 |
| MS.gene011346.t1 | MTR_5g075390 | 60.000 | 70 | 22 | 1 | 19 | 82 | 141 | 210 | 3.97e-15 | 69.7 |
| MS.gene011346.t1 | MTR_6g034945 | 70.000 | 50 | 15 | 0 | 33 | 82 | 153 | 202 | 9.07e-14 | 65.9 |
| MS.gene011346.t1 | MTR_3g094020 | 64.000 | 50 | 18 | 0 | 33 | 82 | 110 | 159 | 7.19e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011346.t1 | AT4G38900 | 67.213 | 61 | 19 | 1 | 23 | 82 | 385 | 445 | 2.95e-16 | 73.6 |
| MS.gene011346.t1 | AT4G38900 | 67.213 | 61 | 19 | 1 | 23 | 82 | 385 | 445 | 2.95e-16 | 73.6 |
| MS.gene011346.t1 | AT4G38900 | 82.222 | 45 | 8 | 0 | 38 | 82 | 407 | 451 | 3.73e-16 | 73.2 |
| MS.gene011346.t1 | AT1G06070 | 72.000 | 50 | 14 | 0 | 33 | 82 | 212 | 261 | 2.43e-15 | 70.9 |
| MS.gene011346.t1 | AT1G06850 | 71.429 | 49 | 14 | 0 | 33 | 81 | 150 | 198 | 7.33e-15 | 68.2 |
| MS.gene011346.t1 | AT1G06850 | 62.903 | 62 | 18 | 1 | 33 | 94 | 150 | 206 | 1.56e-14 | 68.2 |
| MS.gene011346.t1 | AT2G40620 | 68.000 | 50 | 16 | 0 | 33 | 82 | 150 | 199 | 8.88e-14 | 66.2 |
| MS.gene011346.t1 | AT1G43700 | 66.000 | 50 | 17 | 0 | 33 | 82 | 196 | 245 | 9.00e-14 | 66.2 |
| MS.gene011346.t1 | AT2G21230 | 60.000 | 65 | 16 | 1 | 28 | 82 | 357 | 421 | 1.02e-13 | 66.2 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.04e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G31370 | 72.000 | 50 | 14 | 0 | 33 | 82 | 203 | 252 | 1.07e-13 | 65.9 |
| MS.gene011346.t1 | AT2G21230 | 60.000 | 65 | 16 | 1 | 28 | 82 | 357 | 421 | 1.70e-13 | 65.5 |
| MS.gene011346.t1 | AT2G21230 | 60.000 | 65 | 16 | 1 | 28 | 82 | 357 | 421 | 1.70e-13 | 65.5 |
| MS.gene011346.t1 | AT2G21230 | 60.000 | 65 | 16 | 1 | 28 | 82 | 357 | 421 | 1.71e-13 | 65.5 |
Find 18 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACAACTTACTTTGTTGC+AGG | 0.290633 | 8.4:-21430095 | MS.gene011346:intron |
| CTTTCCCTTCATATTGTTGT+TGG | 0.300162 | 8.4:-21429092 | MS.gene011346:CDS |
| TTGAACTAGGTTAGGAAAGT+TGG | 0.356724 | 8.4:-21430802 | MS.gene011346:intron |
| AAGGAATGGAATGGATATAA+AGG | 0.396597 | 8.4:-21430775 | MS.gene011346:intron |
| GCGCAAGATGCAATACATTT+CGG | 0.446357 | 8.4:-21430167 | MS.gene011346:CDS |
| GCTCAAAGGAGTACAAAATC+TGG | 0.448935 | 8.4:+21429020 | None:intergenic |
| TTCTCAGCCTTAGCAATGAA+TGG | 0.462645 | 8.4:+21430626 | None:intergenic |
| AACTAGGTTAGGAAAGTTGG+TGG | 0.467839 | 8.4:-21430799 | MS.gene011346:intron |
| CTAAACCAACAACAATATGA+AGG | 0.504261 | 8.4:+21429087 | None:intergenic |
| GGAAAGTTGGTGGAAAGGAA+TGG | 0.542149 | 8.4:-21430789 | MS.gene011346:CDS |
| CATTTCGGAGCTAGAACAGA+AGG | 0.562989 | 8.4:-21430152 | MS.gene011346:CDS |
| GTTGGTGGAAAGGAATGGAA+TGG | 0.566497 | 8.4:-21430784 | MS.gene011346:CDS |
| GGTTAGGAAAGTTGGTGGAA+AGG | 0.567891 | 8.4:-21430794 | MS.gene011346:CDS |
| TTAGCTAACACATGTATGAC+AGG | 0.571307 | 8.4:-21429068 | MS.gene011346:CDS |
| TAAGGCTCCATTCATTGCTA+AGG | 0.574542 | 8.4:-21430633 | MS.gene011346:CDS |
| TAAACCAACAACAATATGAA+GGG | 0.576531 | 8.4:+21429088 | None:intergenic |
| TAAGGCTGAGAAGTTGAAAG+AGG | 0.582193 | 8.4:-21430615 | MS.gene011346:CDS |
| AAGTTGTGCAGAAAGTGTAG+TGG | 0.665732 | 8.4:+21430108 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATTTCATAAAAAGAA+TGG | - | chr8.4:21430653-21430672 | MS.gene011346:intron | 10.0% |
| !! | AAAATAAATAAGCAATATGA+AGG | + | chr8.4:21430817-21430836 | None:intergenic | 15.0% |
| !! | AAATAAATAAGCAATATGAA+GGG | + | chr8.4:21430816-21430835 | None:intergenic | 15.0% |
| !!! | AGATATTTGTTAATAGAAAT+GGG | + | chr8.4:21430019-21430038 | None:intergenic | 15.0% |
| !!! | TAGATATTTGTTAATAGAAA+TGG | + | chr8.4:21430020-21430039 | None:intergenic | 15.0% |
| !!! | TTTTAGTATATTTTTTCGTT+CGG | - | chr8.4:21430488-21430507 | MS.gene011346:intron | 15.0% |
| !!! | TTTTCGTTGAATTTATTTTT+AGG | - | chr8.4:21429071-21429090 | MS.gene011346:CDS | 15.0% |
| !! | AAAATTAAGCTAAAATGAGA+AGG | - | chr8.4:21430256-21430275 | MS.gene011346:intron | 20.0% |
| !! | AAATTAAGCTAAAATGAGAA+GGG | - | chr8.4:21430257-21430276 | MS.gene011346:intron | 20.0% |
| !! | ATAGACATCTATACTATTAT+TGG | - | chr8.4:21430202-21430221 | MS.gene011346:CDS | 20.0% |
| !! | CAAAAAAATAAATCCAAAAC+TGG | + | chr8.4:21430356-21430375 | None:intergenic | 20.0% |
| !! | TAGACATCTATACTATTATT+GGG | - | chr8.4:21430203-21430222 | MS.gene011346:CDS | 20.0% |
| !! | TCTTATTGAACATTTAATGA+AGG | - | chr8.4:21429984-21430003 | MS.gene011346:intron | 20.0% |
| !! | TTTAGTTAGTAATGTCAATA+TGG | - | chr8.4:21429670-21429689 | MS.gene011346:intron | 20.0% |
| !!! | AGTTTAGTTTTTAACACAAA+AGG | - | chr8.4:21430122-21430141 | MS.gene011346:CDS | 20.0% |
| !!! | ATTGTTTCTTATATCTGAAA+TGG | - | chr8.4:21430081-21430100 | MS.gene011346:intron | 20.0% |
| !!! | ATTTTTTTTTGGGTTATTTC+AGG | - | chr8.4:21429713-21429732 | MS.gene011346:intron | 20.0% |
| !!! | CAGTTTTGGATTTATTTTTT+TGG | - | chr8.4:21430354-21430373 | MS.gene011346:intron | 20.0% |
| !!! | TGACTTGGATATTTTTTTTT+GGG | - | chr8.4:21429703-21429722 | MS.gene011346:intron | 20.0% |
| !!! | TTGACTTGGATATTTTTTTT+TGG | - | chr8.4:21429702-21429721 | MS.gene011346:intron | 20.0% |
| !!! | TTGTGATTTATGTGTATTTT+AGG | - | chr8.4:21429284-21429303 | MS.gene011346:intron | 20.0% |
| !!! | TTTATGTGTATTTTAGGATA+AGG | - | chr8.4:21429290-21429309 | MS.gene011346:intron | 20.0% |
| ! | AATAGTATAGATGTCTATCA+AGG | + | chr8.4:21430200-21430219 | None:intergenic | 25.0% |
| ! | AATTAAGCTAAAATGAGAAG+GGG | - | chr8.4:21430258-21430277 | MS.gene011346:intron | 25.0% |
| ! | ACAATGTCAAATTGATGTAT+AGG | + | chr8.4:21430234-21430253 | None:intergenic | 25.0% |
| ! | ATGGATATAAAGGTTGAATT+AGG | - | chr8.4:21429176-21429195 | MS.gene011346:intron | 25.0% |
| ! | CAGATAAATTACATAGACAA+TGG | + | chr8.4:21430294-21430313 | None:intergenic | 25.0% |
| ! | TAAACCAACAACAATATGAA+GGG | + | chr8.4:21430856-21430875 | None:intergenic | 25.0% |
| ! | TATAAAGGTTGAATTAGGTT+AGG | - | chr8.4:21429181-21429200 | MS.gene011346:intron | 25.0% |
| ! | TGTAAAACTACTCAAATCAT+AGG | + | chr8.4:21429957-21429976 | None:intergenic | 25.0% |
| ! | TTCAAATAATCCTCAAAACA+TGG | + | chr8.4:21429899-21429918 | None:intergenic | 25.0% |
| ! | TTCATTGAACATTGCAAATT+TGG | + | chr8.4:21429930-21429949 | None:intergenic | 25.0% |
| ! | TTCGGAATTGAAGAAAATTA+TGG | - | chr8.4:21429570-21429589 | MS.gene011346:intron | 25.0% |
| ! | TTTACTTCTCTTATGTTACT+TGG | - | chr8.4:21429423-21429442 | MS.gene011346:intron | 25.0% |
| ! | TTTCATTATCTCCATATCAA+AGG | + | chr8.4:21430737-21430756 | None:intergenic | 25.0% |
| !! | AATTTTCTTCAATGAAACAG+AGG | + | chr8.4:21429224-21429243 | None:intergenic | 25.0% |
| !! | GAGTTTTCTAACGTAATTTA+CGG | - | chr8.4:21429111-21429130 | MS.gene011346:intron | 25.0% |
| !! | TACAAATGAATCAGATTTTG+AGG | - | chr8.4:21430684-21430703 | MS.gene011346:intron | 25.0% |
| !! | TTATATCTGAAATGGACAAA+AGG | - | chr8.4:21430089-21430108 | MS.gene011346:intron | 25.0% |
| !!! | AAATATGCTAAAAGCATTCA+TGG | + | chr8.4:21430047-21430066 | None:intergenic | 25.0% |
| !!! | TGGATTTATTTTTTTGGTGA+AGG | - | chr8.4:21430360-21430379 | MS.gene011346:intron | 25.0% |
| AAAGACACTACTTGTGAAAA+TGG | + | chr8.4:21430600-21430619 | None:intergenic | 30.0% | |
| AAGGAATGGAATGGATATAA+AGG | - | chr8.4:21429166-21429185 | MS.gene011346:intron | 30.0% | |
| ATATGAAGGGAAAAATTCCA+TGG | + | chr8.4:21430803-21430822 | None:intergenic | 30.0% | |
| ATTATTGTCATTGCAAAGTG+AGG | + | chr8.4:21430392-21430411 | None:intergenic | 30.0% | |
| ATTTACGGTGAATTTGAACT+AGG | - | chr8.4:21429126-21429145 | MS.gene011346:intron | 30.0% | |
| CTAAACCAACAACAATATGA+AGG | + | chr8.4:21430857-21430876 | None:intergenic | 30.0% | |
| GTTAGTAATGTCAATATGGA+TGG | - | chr8.4:21429674-21429693 | MS.gene011346:intron | 30.0% | |
| ! | TATGGATGGTTAATTTGACT+TGG | - | chr8.4:21429688-21429707 | MS.gene011346:intron | 30.0% |
| !! | AAATCGATTTACCGTTTTTG+TGG | - | chr8.4:21429026-21429045 | MS.gene011346:CDS | 30.0% |
| !! | ATTTCAGGATTTTGAGAAAC+AGG | - | chr8.4:21429728-21429747 | MS.gene011346:intron | 30.0% |
| AAAGGAACATGAAGACAGAT+AGG | + | chr8.4:21430719-21430738 | None:intergenic | 35.0% | |
| ACAATGCGTGCTTACAATAT+GGG | - | chr8.4:21429360-21429379 | MS.gene011346:intron | 35.0% | |
| CACAAAACTCACCACAAAAA+CGG | + | chr8.4:21429040-21429059 | None:intergenic | 35.0% | |
| CTTTCCCTTCATATTGTTGT+TGG | - | chr8.4:21430849-21430868 | MS.gene011346:intron | 35.0% | |
| GAAAGTTGCTGAGATTGTTA+TGG | - | chr8.4:21429600-21429619 | MS.gene011346:intron | 35.0% | |
| GAGAGAGTAATAACTTTAGC+TGG | + | chr8.4:21430563-21430582 | None:intergenic | 35.0% | |
| GTCTTCATGTTCCTTTGATA+TGG | - | chr8.4:21430723-21430742 | MS.gene011346:intron | 35.0% | |
| TTAGACTGAGAAGAATGCTT+CGG | - | chr8.4:21429488-21429507 | MS.gene011346:intron | 35.0% | |
| TTAGCTAACACATGTATGAC+AGG | - | chr8.4:21430873-21430892 | MS.gene011346:intron | 35.0% | |
| TTGAACATTGCAAATTTGGC+AGG | + | chr8.4:21429926-21429945 | None:intergenic | 35.0% | |
| TTGAACTAGGTTAGGAAAGT+TGG | - | chr8.4:21429139-21429158 | MS.gene011346:intron | 35.0% | |
| ! | GTTTGTGAAACCATGTTTTG+AGG | - | chr8.4:21429886-21429905 | MS.gene011346:intron | 35.0% |
| !! | GATTTTGAGAAACAGGCAAT+CGG | - | chr8.4:21429735-21429754 | MS.gene011346:intron | 35.0% |
| !!! | GCTGGAGTTGTTTTTACAAT+AGG | + | chr8.4:21430545-21430564 | None:intergenic | 35.0% |
| !!! | TATAGTGCTGATACCAGTTT+TGG | - | chr8.4:21430340-21430359 | MS.gene011346:intron | 35.0% |
| AACTAGGTTAGGAAAGTTGG+TGG | - | chr8.4:21429142-21429161 | MS.gene011346:intron | 40.0% | |
| ACAACTTACTTTGTTGCAGG+TGG | - | chr8.4:21429849-21429868 | MS.gene011346:intron | 40.0% | |
| CAACTTACTTTGTTGCAGGT+GGG | - | chr8.4:21429850-21429869 | MS.gene011346:intron | 40.0% | |
| CGGTGAATTTGAACTAGGTT+AGG | - | chr8.4:21429131-21429150 | MS.gene011346:intron | 40.0% | |
| GACAATGCGTGCTTACAATA+TGG | - | chr8.4:21429359-21429378 | MS.gene011346:intron | 40.0% | |
| GACACTACTTGTGAAAATGG+TGG | + | chr8.4:21430597-21430616 | None:intergenic | 40.0% | |
| GCGCAAGATGCAATACATTT+CGG | - | chr8.4:21429774-21429793 | MS.gene011346:intron | 40.0% | |
| TAAGGCTCCATTCATTGCTA+AGG | - | chr8.4:21429308-21429327 | MS.gene011346:intron | 40.0% | |
| TAAGGCTGAGAAGTTGAAAG+AGG | - | chr8.4:21429326-21429345 | MS.gene011346:intron | 40.0% | |
| TGCACAACTTACTTTGTTGC+AGG | - | chr8.4:21429846-21429865 | MS.gene011346:intron | 40.0% | |
| TTCTCAGCCTTAGCAATGAA+TGG | + | chr8.4:21429318-21429337 | None:intergenic | 40.0% | |
| ! | AAGTTGTGCAGAAAGTGTAG+TGG | + | chr8.4:21429836-21429855 | None:intergenic | 40.0% |
| ! | CTGTATCAACTGTCGTTTCA+TGG | - | chr8.4:21430311-21430330 | MS.gene011346:intron | 40.0% |
| ! | TGCTGCTCTATTTTCTACCA+TGG | - | chr8.4:21430783-21430802 | MS.gene011346:CDS | 40.0% |
| AAGAATGCTTCGGAGGAAGA+AGG | - | chr8.4:21429498-21429517 | MS.gene011346:intron | 45.0% | |
| CATTTCGGAGCTAGAACAGA+AGG | - | chr8.4:21429789-21429808 | MS.gene011346:intron | 45.0% | |
| GGAACATGAAGACAGATAGG+AGG | + | chr8.4:21430716-21430735 | None:intergenic | 45.0% | |
| TTGTTATGGCTGATCCGAAG+CGG | - | chr8.4:21429614-21429633 | MS.gene011346:intron | 45.0% | |
| ! | GTTGGTGGAAAGGAATGGAA+TGG | - | chr8.4:21429157-21429176 | MS.gene011346:intron | 45.0% |
| !! | GGAAAGTTGGTGGAAAGGAA+TGG | - | chr8.4:21429152-21429171 | MS.gene011346:intron | 45.0% |
| !! | GGTTAGGAAAGTTGGTGGAA+AGG | - | chr8.4:21429147-21429166 | MS.gene011346:intron | 45.0% |
| GACTGAGAAGAATGCTTCGG+AGG | - | chr8.4:21429491-21429510 | MS.gene011346:intron | 50.0% | |
| TGTTATGGCTGATCCGAAGC+GGG | - | chr8.4:21429615-21429634 | MS.gene011346:intron | 50.0% | |
| CAACACCTCTTTGCCCGCTT+CGG | + | chr8.4:21429631-21429650 | None:intergenic | 55.0% | |
| CTGATCCGAAGCGGGCAAAG+AGG | - | chr8.4:21429623-21429642 | MS.gene011346:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 21429022 | 21430941 | 21429022 | ID=MS.gene011346 |
| chr8.4 | mRNA | 21429022 | 21430941 | 21429022 | ID=MS.gene011346.t1;Parent=MS.gene011346 |
| chr8.4 | exon | 21430916 | 21430941 | 21430916 | ID=MS.gene011346.t1.exon1;Parent=MS.gene011346.t1 |
| chr8.4 | CDS | 21430916 | 21430941 | 21430916 | ID=cds.MS.gene011346.t1;Parent=MS.gene011346.t1 |
| chr8.4 | exon | 21430776 | 21430815 | 21430776 | ID=MS.gene011346.t1.exon2;Parent=MS.gene011346.t1 |
| chr8.4 | CDS | 21430776 | 21430815 | 21430776 | ID=cds.MS.gene011346.t1;Parent=MS.gene011346.t1 |
| chr8.4 | exon | 21430614 | 21430657 | 21430614 | ID=MS.gene011346.t1.exon3;Parent=MS.gene011346.t1 |
| chr8.4 | CDS | 21430614 | 21430657 | 21430614 | ID=cds.MS.gene011346.t1;Parent=MS.gene011346.t1 |
| chr8.4 | exon | 21430096 | 21430228 | 21430096 | ID=MS.gene011346.t1.exon4;Parent=MS.gene011346.t1 |
| chr8.4 | CDS | 21430096 | 21430228 | 21430096 | ID=cds.MS.gene011346.t1;Parent=MS.gene011346.t1 |
| chr8.4 | exon | 21429022 | 21429120 | 21429022 | ID=MS.gene011346.t1.exon5;Parent=MS.gene011346.t1 |
| chr8.4 | CDS | 21429022 | 21429120 | 21429022 | ID=cds.MS.gene011346.t1;Parent=MS.gene011346.t1 |
| Gene Sequence |
| Protein sequence |
