AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011425

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011425.t1 MTR_4g102790 98.380 679 8 2 1 678 1 677 0.0 1375
MS.gene011425.t1 MTR_7g027190 37.722 395 223 7 281 662 288 672 9.81e-81 270
MS.gene011425.t1 MTR_7g074650 36.856 369 215 10 297 664 442 793 3.23e-63 225
MS.gene011425.t1 MTR_1g069725 33.810 420 247 11 256 663 63 463 1.06e-58 205
MS.gene011425.t1 MTR_5g094450 33.418 395 227 10 286 662 155 531 5.51e-58 205
MS.gene011425.t1 MTR_4g104020 34.367 387 233 13 287 662 143 519 9.62e-57 201
MS.gene011425.t1 MTR_8g442410 29.259 499 317 12 185 662 41 524 3.68e-56 200
MS.gene011425.t1 MTR_3g065980 31.328 399 236 8 282 662 161 539 1.22e-55 199
MS.gene011425.t1 MTR_3g089055 36.126 382 217 14 293 662 198 564 1.72e-55 199
MS.gene011425.t1 MTR_3g089055 36.126 382 217 14 293 662 203 569 1.79e-55 199
MS.gene011425.t1 MTR_1g096030 33.088 408 226 14 283 662 70 458 1.08e-54 194
MS.gene011425.t1 MTR_1g029420 35.079 382 221 13 293 662 225 591 4.66e-54 195
MS.gene011425.t1 MTR_4g076140 28.689 488 266 11 236 662 2 468 5.58e-54 192
MS.gene011425.t1 MTR_2g082090 32.282 412 244 13 269 662 184 578 4.27e-53 192
MS.gene011425.t1 MTR_5g097480 33.508 382 233 11 290 662 174 543 5.45e-53 191
MS.gene011425.t1 MTR_3g056110 30.769 403 249 9 273 662 156 541 8.30e-51 185
MS.gene011425.t1 MTR_3g022830 27.725 422 240 9 294 662 26 435 4.21e-47 172
MS.gene011425.t1 MTR_4g076020 33.245 376 216 14 297 667 88 433 5.09e-47 172
MS.gene011425.t1 MTR_2g097410 28.979 421 274 11 258 664 333 742 6.06e-46 174
MS.gene011425.t1 MTR_4g133660 29.610 385 249 9 288 662 170 542 5.12e-45 169
MS.gene011425.t1 MTR_2g097310 26.302 384 263 8 289 664 268 639 6.29e-45 171
MS.gene011425.t1 MTR_2g026250 28.319 452 299 12 221 662 159 595 1.36e-44 169
MS.gene011425.t1 MTR_2g097380 27.763 371 223 9 300 664 231 562 6.23e-44 166
MS.gene011425.t1 MTR_4g097080 27.249 389 251 9 292 664 132 504 8.15e-44 165
MS.gene011425.t1 MTR_4g077760 31.152 382 237 12 290 662 190 554 9.27e-44 166
MS.gene011425.t1 MTR_2g097390 26.684 386 259 9 289 664 271 642 1.92e-43 166
MS.gene011425.t1 MTR_4g064160 27.040 429 288 10 250 664 268 685 1.88e-41 161
MS.gene011425.t1 MTR_4g064120 27.404 416 274 11 266 664 223 627 6.31e-41 159
MS.gene011425.t1 MTR_4g064150 27.273 407 268 12 274 665 342 735 1.77e-40 158
MS.gene011425.t1 MTR_2g097463 24.031 516 326 12 197 664 159 656 1.92e-40 157
MS.gene011425.t1 MTR_5g009080 32.443 262 166 2 239 500 9 259 2.49e-40 154
MS.gene011425.t1 MTR_5g009080 33.333 153 95 5 511 662 331 477 6.91e-19 90.5
MS.gene011425.t1 MTR_5g058860 31.785 409 239 16 279 663 105 497 2.77e-40 155
MS.gene011425.t1 MTR_3g072710 31.127 408 237 16 283 663 110 500 2.94e-40 155
MS.gene011425.t1 MTR_4g064180 27.525 396 263 10 282 664 243 627 4.17e-40 156
MS.gene011425.t1 MTR_2g097350 25.515 388 261 7 289 664 270 641 4.63e-40 156
MS.gene011425.t1 MTR_5g015490 28.608 388 253 11 292 664 113 491 9.06e-40 153
MS.gene011425.t1 MTR_4g064200 26.494 385 261 9 289 664 280 651 1.22e-39 155
MS.gene011425.t1 MTR_2g034250 28.109 402 268 10 276 665 195 587 1.75e-39 154
MS.gene011425.t1 MTR_2g034260 27.930 401 263 11 276 662 195 583 2.01e-37 148
MS.gene011425.t1 MTR_2g034280 27.820 399 264 12 276 662 188 574 2.10e-37 147
MS.gene011425.t1 MTR_2g097467 24.936 389 265 9 287 664 283 655 3.21e-37 148
MS.gene011425.t1 MTR_7g062120 24.936 389 264 8 289 664 368 741 1.05e-36 147
MS.gene011425.t1 MTR_8g093070 29.383 405 241 16 278 666 124 499 2.97e-36 143
MS.gene011425.t1 MTR_7g109580 26.992 389 263 10 282 662 178 553 5.09e-36 143
MS.gene011425.t1 MTR_2g089100 25.616 406 261 10 293 667 52 447 1.37e-35 140
MS.gene011425.t1 MTR_2g097473 24.524 420 279 11 267 664 252 655 5.61e-35 141
MS.gene011425.t1 MTR_1g086970 24.359 390 279 10 282 662 95 477 3.25e-34 137
MS.gene011425.t1 MTR_2g099110 25.679 405 242 10 293 664 74 452 5.24e-32 130
MS.gene011425.t1 MTR_7g057230 34.221 263 160 10 405 662 402 656 2.30e-31 130
MS.gene011425.t1 MTR_5g015950 24.048 420 264 13 293 664 35 447 1.67e-25 110
MS.gene011425.t1 MTR_3g053270 24.048 420 264 13 293 664 35 447 1.67e-25 110
MS.gene011425.t1 MTR_6g047750 32.222 270 162 10 405 662 401 661 1.74e-25 112
MS.gene011425.t1 MTR_4g026485 25.526 380 246 11 294 662 270 623 1.05e-24 109
MS.gene011425.t1 MTR_0092s0100 24.814 403 277 9 266 662 345 727 2.63e-23 105
MS.gene011425.t1 MTR_0092s0100 24.378 402 280 7 266 662 348 730 2.88e-23 105
MS.gene011425.t1 MTR_4g095500 22.673 419 269 13 293 664 59 469 3.96e-19 91.3
MS.gene011425.t1 MTR_8g077940 27.577 359 218 14 319 662 209 540 3.72e-17 85.5
MS.gene011425.t1 MTR_8g020840 23.786 412 237 15 293 662 171 547 1.60e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011425.t1 AT1G63100 55.777 701 225 23 1 663 1 654 0.0 689
MS.gene011425.t1 AT1G63100 53.638 701 215 21 1 663 1 629 0.0 656
MS.gene011425.t1 AT1G66350 34.961 389 218 11 282 662 144 505 5.87e-67 229
MS.gene011425.t1 AT2G01570 35.990 389 220 11 282 662 213 580 1.97e-65 226
MS.gene011425.t1 AT1G50420 32.360 445 231 10 279 662 43 478 5.26e-65 223
MS.gene011425.t1 AT1G14920 35.219 389 223 11 282 662 161 528 1.08e-64 223
MS.gene011425.t1 AT3G54220 34.949 392 238 8 273 664 277 651 1.70e-64 226
MS.gene011425.t1 AT3G03450 33.162 389 236 11 282 662 172 544 1.55e-61 215
MS.gene011425.t1 AT5G17490 33.073 384 233 11 284 662 151 515 1.03e-59 209
MS.gene011425.t1 AT5G41920 35.641 390 228 13 278 662 28 399 5.75e-59 204
MS.gene011425.t1 AT1G55580 35.036 411 222 12 290 662 41 444 3.72e-53 189
MS.gene011425.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.15e-48 178
MS.gene011425.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.15e-48 178
MS.gene011425.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.15e-48 178
MS.gene011425.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.15e-48 178
MS.gene011425.t1 AT5G66770 32.172 373 217 12 302 662 235 583 1.59e-48 180
MS.gene011425.t1 AT2G04890 32.540 378 237 12 289 662 49 412 5.47e-48 174
MS.gene011425.t1 AT1G50600 31.552 393 242 12 285 662 145 525 9.39e-48 176
MS.gene011425.t1 AT1G50600 31.980 394 239 13 285 662 216 596 3.00e-47 176
MS.gene011425.t1 AT1G50600 31.980 394 239 13 285 662 220 600 3.61e-47 176
MS.gene011425.t1 AT4G17230 32.041 387 240 10 288 664 153 526 1.82e-46 173
MS.gene011425.t1 AT4G17230 32.041 387 240 10 288 664 153 526 1.82e-46 173
MS.gene011425.t1 AT1G21450 30.900 411 256 11 268 662 194 592 1.39e-45 172
MS.gene011425.t1 AT2G29065 31.026 390 241 13 292 664 251 629 1.38e-42 164
MS.gene011425.t1 AT3G50650 32.609 368 209 14 313 662 195 541 1.68e-42 162
MS.gene011425.t1 AT2G29060 27.619 420 265 12 258 665 265 657 2.69e-41 160
MS.gene011425.t1 AT2G29060 27.619 420 265 12 258 665 302 694 3.30e-41 160
MS.gene011425.t1 AT1G07530 27.979 386 257 10 289 664 392 766 1.99e-40 159
MS.gene011425.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.70e-40 154
MS.gene011425.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.70e-40 154
MS.gene011425.t1 AT1G07520 28.288 403 254 16 282 664 307 694 1.80e-39 155
MS.gene011425.t1 AT5G59450 25.887 479 310 14 220 666 136 601 1.88e-39 154
MS.gene011425.t1 AT1G07520 28.288 403 254 16 282 664 308 695 2.06e-39 155
MS.gene011425.t1 AT4G37650 27.481 393 258 9 292 664 145 530 3.50e-39 152
MS.gene011425.t1 AT4G08250 28.856 402 250 14 279 662 93 476 4.83e-39 151
MS.gene011425.t1 AT3G46600 26.877 413 269 10 261 664 192 580 1.75e-36 145
MS.gene011425.t1 AT3G46600 28.010 382 254 9 292 664 3 372 2.15e-36 141
MS.gene011425.t1 AT3G46600 27.119 413 273 10 268 664 148 548 5.49e-36 143
MS.gene011425.t1 AT2G37650 25.700 393 254 11 289 664 331 702 3.24e-32 133
MS.gene011425.t1 AT2G37650 25.700 393 254 11 289 664 343 714 4.29e-32 133
MS.gene011425.t1 AT5G52510 29.283 321 193 10 367 662 328 639 3.10e-27 117
MS.gene011425.t1 AT3G49950 26.700 397 251 12 293 665 30 410 4.83e-26 111
MS.gene011425.t1 AT4G00150 28.011 357 222 12 312 662 226 553 3.54e-25 110
MS.gene011425.t1 AT3G60630 27.597 308 196 12 369 662 327 621 1.06e-23 106
MS.gene011425.t1 AT2G45160 27.302 315 185 11 369 662 347 638 1.92e-21 99.8
MS.gene011425.t1 AT4G36710 23.650 389 261 12 287 662 119 484 1.88e-18 89.4
MS.gene011425.t1 AT3G13840 24.939 413 250 18 275 662 132 509 4.04e-17 85.5

Find 171 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCATTTCATTGCAAAAT+TGG 0.138280 8.2:+20926143 MS.gene011425:CDS
CCTTTCTCTTTGAATTGTTC+TGG 0.202054 8.2:+20925745 MS.gene011425:CDS
ACTGGTTGTGTTGATGCTAT+TGG 0.221753 8.2:+20926099 MS.gene011425:CDS
GTAACAAAGATCACCAATTT+TGG 0.231391 8.2:+20925853 MS.gene011425:CDS
CTGCTGCTGTTCTTGACTTC+AGG 0.245846 8.2:-20925421 None:intergenic
AGATCAAGGATGAGGAGTTT+TGG 0.247813 8.2:+20925512 MS.gene011425:CDS
AGTTTCAGTTTGGCACAATT+TGG 0.253074 8.2:+20925661 MS.gene011425:CDS
TTGGAGAGGCACCAGAGTTT+TGG 0.253860 8.2:+20926984 MS.gene011425:CDS
CTCTCTACACAGTATCTTCT+TGG 0.270369 8.2:+20927180 MS.gene011425:CDS
TGTCACCAACTCACAGATTA+AGG 0.272810 8.2:+20925248 MS.gene011425:CDS
AACCATTTCATTGCAAAATT+GGG 0.272931 8.2:+20926144 MS.gene011425:CDS
CTGGTTGTGTTGATGCTATT+GGG 0.293519 8.2:+20926100 MS.gene011425:CDS
AAATAGAGCTAAGAGGAAAA+AGG 0.307901 8.2:+20925597 MS.gene011425:CDS
ATAAGCACAAATTCGGCTTA+TGG 0.317498 8.2:-20926193 None:intergenic
GCTTGCAGAACATGGATCTT+TGG 0.333074 8.2:+20925561 MS.gene011425:CDS
CAATCCTACAATTGTTGTTA+TGG 0.340164 8.2:+20926770 MS.gene011425:CDS
TGGACACCGGTTGACGTTGC+TGG 0.348429 8.2:+20927200 MS.gene011425:CDS
CATGAAGAGGGTAATGGTTC+GGG 0.351755 8.2:+20925985 MS.gene011425:CDS
TGCCTTTGTCTAACAATCCT+TGG 0.358700 8.2:+20925818 MS.gene011425:CDS
AATACATCTTCAACAGAAAC+TGG 0.362646 8.2:-20927082 None:intergenic
TCAGTTCCTTCATGCTGATA+AGG 0.365523 8.2:-20926012 None:intergenic
CGATGTGAAATGAAGAAACT+TGG 0.373450 8.2:-20926361 None:intergenic
TCTGCCATAACAACAATTGT+AGG 0.376086 8.2:-20926774 None:intergenic
GTGGCTACCCTCACCAAAAT+TGG 0.379531 8.2:-20925866 None:intergenic
AATCTCTGTGTGCTCATTGA+TGG 0.381588 8.2:-20925313 None:intergenic
AATTTGGAGCTGGAAACTTT+TGG 0.382164 8.2:+20925677 MS.gene011425:CDS
TGAAAGGGAAAGTTTCAGTT+TGG 0.386013 8.2:+20925651 MS.gene011425:CDS
CGGTAAAATAAGCACAAATT+CGG 0.394214 8.2:-20926200 None:intergenic
GGAGCAACAGCACTTACTTT+GGG 0.396486 8.2:+20927143 MS.gene011425:CDS
ATCAATGAGCACACAGAGAT+TGG 0.409860 8.2:+20925315 MS.gene011425:CDS
GATGCATGGGAGTCACTGAT+AGG 0.410356 8.2:+20927045 MS.gene011425:CDS
GACTTGAAGATGTAAGGCTT+TGG 0.411643 8.2:+20926628 MS.gene011425:CDS
TGTTCCAAAGTTTAGCTTCA+AGG 0.419556 8.2:+20926472 MS.gene011425:CDS
CCATCAGAGTCACTAGGCTT+TGG 0.420891 8.2:+20926232 MS.gene011425:CDS
AGCAACAACTGCTCAGACAT+TGG 0.422074 8.2:+20925486 MS.gene011425:CDS
TCTTCAAGTCTGTCGACTAC+TGG 0.429762 8.2:-20926615 None:intergenic
TCGAACATGGCTTGGAGGAT+TGG 0.430304 8.2:-20926496 None:intergenic
ACATGAAGAGGGTAATGGTT+CGG 0.431411 8.2:+20925984 MS.gene011425:CDS
TTCGCAGCAGCCGTTGAGGC+CGG 0.436828 8.2:+20925378 MS.gene011425:CDS
AGGAGCAACAGCACTTACTT+TGG 0.437760 8.2:+20927142 MS.gene011425:CDS
AGAGTCAGAGTTTGAGCCTA+AGG 0.440522 8.2:+20925941 MS.gene011425:CDS
GACTCTGATGGCTAAGGCTT+CGG 0.442125 8.2:-20926220 None:intergenic
AAATGAAGAAACTTGGGAAT+TGG 0.443639 8.2:-20926354 None:intergenic
GCAACAACTGCTCAGACATT+GGG 0.444891 8.2:+20925487 MS.gene011425:CDS
AGATGTTAGCTAGGGAGATA+AGG 0.446179 8.2:+20926931 MS.gene011425:CDS
AAGCAAGAGCTGAATGAAAC+CGG 0.462075 8.2:+20926540 MS.gene011425:CDS
TGGCCCTTGAAGCTAAACTT+TGG 0.467104 8.2:-20926476 None:intergenic
CCAGAACAATTCAAAGAGAA+AGG 0.469406 8.2:-20925745 None:intergenic
GAGCTTCCAGCAACGTCAAC+CGG 0.470552 8.2:-20927206 None:intergenic
GTTCTCTGCAGCAGAACATT+CGG 0.471882 8.2:+20925452 MS.gene011425:CDS
TTGTTCTGGAGATGAGGAAA+GGG 0.476638 8.2:+20925759 MS.gene011425:CDS
GAAACCGGAGACAGACTTGC+TGG 0.481646 8.2:+20926555 MS.gene011425:CDS
CATAACAACAATTGTAGGAT+TGG 0.483005 8.2:-20926769 None:intergenic
AGATAGAGGAGATGTTAGCT+AGG 0.487005 8.2:+20926922 MS.gene011425:CDS
CCAAGCCATGTTCGAATCAC+GGG 0.489720 8.2:+20926504 MS.gene011425:CDS
TGATCACAGTGGCCTTCCAC+TGG 0.491665 8.2:+20926881 MS.gene011425:CDS
TCAGACATTGGGAGAGATCA+AGG 0.493655 8.2:+20925498 MS.gene011425:CDS
CCATGTTCGAATCACGGGTG+TGG 0.493749 8.2:+20926509 MS.gene011425:CDS
TCCGATGAGGATCAAGATAG+AGG 0.495969 8.2:+20926908 MS.gene011425:CDS
TGCATTGAGGCTTCTAAACC+AGG 0.497162 8.2:+20926326 MS.gene011425:CDS
CACAGTATCTTCTTGGACAC+CGG 0.497435 8.2:+20927187 MS.gene011425:CDS
CATGTTCGAATCACGGGTGT+GGG 0.498098 8.2:+20926510 MS.gene011425:CDS
TCGACAGACTTGAAGATGTA+AGG 0.499051 8.2:+20926622 MS.gene011425:CDS
ATGCATTCTCATTCAACCTT+AGG 0.503609 8.2:-20925957 None:intergenic
CGGCTTATGGAAGTTCCCCT+TGG 0.503709 8.2:-20926180 None:intergenic
GTTCCAAAGTTTAGCTTCAA+GGG 0.504136 8.2:+20926473 MS.gene011425:CDS
GAAGGAACTGAAGTGGAGAC+AGG 0.505317 8.2:+20926024 MS.gene011425:CDS
ATCGAATGAGATGCTGTTGA+GGG 0.506115 8.2:+20926380 MS.gene011425:CDS
CCCGTGATTCGAACATGGCT+TGG 0.506282 8.2:-20926504 None:intergenic
TTGAAGAGGCTTGCAGAACA+TGG 0.509151 8.2:+20925553 MS.gene011425:CDS
ACCATTTCATTGCAAAATTG+GGG 0.509402 8.2:+20926145 MS.gene011425:CDS
TTCTCCAATTCCCAAAACTC+TGG 0.510256 8.2:-20926995 None:intergenic
CAACAGCACTTACTTTGGGT+TGG 0.512066 8.2:+20927147 MS.gene011425:CDS
TCCTCTATCTTGATCCTCAT+CGG 0.513315 8.2:-20926909 None:intergenic
CGCGAAGAACCGTCTTTACG+AGG 0.513686 8.2:-20925355 None:intergenic
CATCGAATGAGATGCTGTTG+AGG 0.514579 8.2:+20926379 MS.gene011425:CDS
AGTTTGGCACAATTTGGAGC+TGG 0.514639 8.2:+20925667 MS.gene011425:CDS
GGTGCAGAAATCACTTCAGC+AGG 0.516593 8.2:-20925793 None:intergenic
GCAGCAGCCGTTGAGGCCGG+TGG 0.517483 8.2:+20925381 MS.gene011425:CDS
GCATGGAGGGTTCAGATGCA+TGG 0.518052 8.2:+20927031 MS.gene011425:CDS
CATGATGATGTTGATGTTGG+TGG 0.518083 8.2:-20925885 None:intergenic
ACTTGGGAATTGGTGTAACC+TGG 0.522388 8.2:-20926344 None:intergenic
AGGAGAAGAGGACGAAGAAG+AGG 0.523774 8.2:+20926044 MS.gene011425:CDS
TTTGAATTGTTCTGGAGATG+AGG 0.524360 8.2:+20925753 MS.gene011425:CDS
CAGTGTTCACAAGCAGGAGC+AGG 0.525223 8.2:+20927118 MS.gene011425:CDS
CAACATCATCATGTCAATGA+AGG 0.526407 8.2:+20925895 MS.gene011425:CDS
AGGGTGGGAGGAGTTTGAAG+AGG 0.527118 8.2:+20925539 MS.gene011425:CDS
GACTACTGGATGAAAATCAA+AGG 0.528709 8.2:-20926601 None:intergenic
CAGCAGCCGTTGAGGCCGGT+GGG 0.533021 8.2:+20925382 MS.gene011425:CDS
GTTACAGCAGATTCAAGCCA+AGG 0.534387 8.2:-20925835 None:intergenic
CTGACTCTCTGATGAACCAT+TGG 0.536463 8.2:-20925926 None:intergenic
TTCAAAGAGAAAGGAATCGA+AGG 0.539186 8.2:-20925736 None:intergenic
TCCAAGCCATGTTCGAATCA+CGG 0.544215 8.2:+20926503 MS.gene011425:CDS
ACATCGTTGCTTGCGAAGGA+AGG 0.546146 8.2:+20926955 MS.gene011425:CDS
GTGATTCGAACATGGCTTGG+AGG 0.550236 8.2:-20926501 None:intergenic
TAATTACAGTGTTCACAAGC+AGG 0.552347 8.2:+20927112 MS.gene011425:CDS
TCACTCCTTAATCTGTGAGT+TGG 0.553015 8.2:-20925253 None:intergenic
AAAACCAGCAAGTCTGTCTC+CGG 0.554388 8.2:-20926559 None:intergenic
TTGCTTGCGAAGGAAGGGAC+AGG 0.554464 8.2:+20926961 MS.gene011425:CDS
ATTGTTCTGGAGATGAGGAA+AGG 0.554859 8.2:+20925758 MS.gene011425:CDS
CATGGAGGGTTCAGATGCAT+GGG 0.555361 8.2:+20927032 MS.gene011425:CDS
AGAAAGATGATAGTGGAGCA+TGG 0.555422 8.2:+20927014 MS.gene011425:CDS
GGAACACAATGAGGCGAGGT+TGG 0.556078 8.2:+20926806 MS.gene011425:CDS
GCTTTGGATGCTTCATGTGA+AGG 0.557282 8.2:+20926644 MS.gene011425:CDS
GATAGAGGAGATGTTAGCTA+GGG 0.557387 8.2:+20926923 MS.gene011425:CDS
AAAGGGAAGGTTCAATGCCT+CGG 0.557666 8.2:-20926583 None:intergenic
ATTGGGAGAGATCAAGGATG+AGG 0.561558 8.2:+20925504 MS.gene011425:CDS
TCAGAACATGAAGAGGGTAA+TGG 0.563710 8.2:+20925979 MS.gene011425:CDS
TCATCGGACTCTCCAGTGGA+AGG 0.563830 8.2:-20926893 None:intergenic
GCTAAACTTTGGAACAAGCT+AGG 0.565024 8.2:-20926465 None:intergenic
AAACCATAGACGATGTTGGC+TGG 0.565074 8.2:+20925205 None:intergenic
CCTTAGCCATCAGAGTCACT+AGG 0.567717 8.2:+20926226 MS.gene011425:CDS
GAATTGGAGAAAGATGATAG+TGG 0.568946 8.2:+20927007 MS.gene011425:CDS
TGACATGATGATGTTGATGT+TGG 0.569622 8.2:-20925888 None:intergenic
ACTACTGGATGAAAATCAAA+GGG 0.570042 8.2:-20926600 None:intergenic
CCACACCCGTGATTCGAACA+TGG 0.572154 8.2:-20926509 None:intergenic
TGAAGTGGAGACAGGAGAAG+AGG 0.572761 8.2:+20926032 MS.gene011425:CDS
GTCTGAGCAGTTGTTGCTAA+AGG 0.573475 8.2:-20925481 None:intergenic
CCTAGTGACTCTGATGGCTA+AGG 0.575424 8.2:-20926226 None:intergenic
AGATGATAGTGGAGCATGGA+GGG 0.575789 8.2:+20927018 MS.gene011425:CDS
GGCAATGAATGAATTCAAGC+TGG 0.576427 8.2:-20927245 None:intergenic
GAAATCCCCACCGGCCTCAA+CGG 0.577034 8.2:-20925388 None:intergenic
AAGACACTTCATGATGGTAG+TGG 0.578818 8.2:+20926708 MS.gene011425:CDS
GATGTGAAATGAAGAAACTT+GGG 0.580703 8.2:-20926360 None:intergenic
TCAGCATGAAGGAACTGAAG+TGG 0.582854 8.2:+20926017 MS.gene011425:CDS
TAGGATTGGTGCTACGAATG+AGG 0.584060 8.2:-20926755 None:intergenic
AGCAGCCGTTGAGGCCGGTG+GGG 0.586757 8.2:+20925383 MS.gene011425:CDS
ATCCTCATCGGACTCTCCAG+TGG 0.587086 8.2:-20926897 None:intergenic
TGTAGCTTCCCTCGTAAAGA+CGG 0.588273 8.2:+20925346 MS.gene011425:CDS
ATGCATGGGAGTCACTGATA+GGG 0.590517 8.2:+20927046 MS.gene011425:CDS
AGCCAAGGATTGTTAGACAA+AGG 0.592331 8.2:-20925820 None:intergenic
AGGAACATCGTTGCTTGCGA+AGG 0.592848 8.2:+20926951 MS.gene011425:CDS
CATCGTTGCTTGCGAAGGAA+GGG 0.593672 8.2:+20926956 MS.gene011425:CDS
CCATTTCATTGCAAAATTGG+GGG 0.595375 8.2:+20926146 MS.gene011425:CDS
TCTTTACGAGGGAAGCTACA+AGG 0.595626 8.2:-20925343 None:intergenic
TGTTCGAATCACGGGTGTGG+GGG 0.596570 8.2:+20926512 MS.gene011425:CDS
ATGTTCGAATCACGGGTGTG+GGG 0.598829 8.2:+20926511 MS.gene011425:CDS
CCAAAGCCTAGTGACTCTGA+TGG 0.602086 8.2:-20926232 None:intergenic
GCAAGAAGCGGAACACAATG+AGG 0.609256 8.2:+20926797 MS.gene011425:CDS
CTGGATGAAAATCAAAGGGA+AGG 0.609858 8.2:-20926596 None:intergenic
CGCGTTCGCAGCAGCCGTTG+AGG 0.611606 8.2:+20925374 MS.gene011425:CDS
GTGTTGATGCTATTGGGTCA+AGG 0.614329 8.2:+20926106 MS.gene011425:CDS
GGGAATCCTTATCAGCATGA+AGG 0.614596 8.2:+20926006 MS.gene011425:CDS
TCAAAGAGAAAGGAATCGAA+GGG 0.615189 8.2:-20925735 None:intergenic
TTGCGAAGGAAGGGACAGGT+TGG 0.618359 8.2:+20926965 MS.gene011425:CDS
GAATGCATCAGAACATGAAG+AGG 0.618408 8.2:+20925972 MS.gene011425:CDS
TATTAAACCCTCCAAAACTG+TGG 0.620782 8.2:-20925705 None:intergenic
AATGAAGGTTCAGTCTCCAA+TGG 0.621452 8.2:+20925910 MS.gene011425:CDS
ACACTTCATGATGGTAGTGG+AGG 0.624635 8.2:+20926711 MS.gene011425:CDS
TGCTATTGGGTCAAGGAATG+TGG 0.631345 8.2:+20926113 MS.gene011425:CDS
CAAGAAGATACTGTGTAGAG+AGG 0.631657 8.2:-20927179 None:intergenic
ATGAAACTGTCACTGCATTG+AGG 0.631836 8.2:+20926313 MS.gene011425:CDS
AAGGAAGGGACAGGTTGGAG+AGG 0.635039 8.2:+20926970 MS.gene011425:CDS
ATCGACTTCGACATAAAGCA+AGG 0.635920 8.2:+20926432 MS.gene011425:CDS
AAGATGATAGTGGAGCATGG+AGG 0.636506 8.2:+20927017 MS.gene011425:CDS
TTCTCAACTGAAATCCCCAC+CGG 0.637454 8.2:-20925397 None:intergenic
GCGAAGAACCGTCTTTACGA+GGG 0.645378 8.2:-20925354 None:intergenic
GTTCACAAGCAGGAGCAGGA+AGG 0.645596 8.2:+20927122 MS.gene011425:CDS
AATGCATCAGAACATGAAGA+GGG 0.650132 8.2:+20925973 MS.gene011425:CDS
CTTCACAAGACACTTCATGA+TGG 0.664343 8.2:+20926702 MS.gene011425:CDS
GGATTGTTAGACAAAGGCAA+AGG 0.667360 8.2:-20925814 None:intergenic
ATGAAGAGGGTAATGGTTCG+GGG 0.669794 8.2:+20925986 MS.gene011425:CDS
TGTTATGGCAGAGCAAGAAG+CGG 0.671377 8.2:+20926785 MS.gene011425:CDS
ACATAAAGCAAGGACTACAA+TGG 0.673543 8.2:+20926442 MS.gene011425:CDS
TGAGCAGTTGTTGCTAAAGG+TGG 0.673874 8.2:-20925478 None:intergenic
GGGGATCTTGCATCTCCAAG+GGG 0.674372 8.2:+20926165 MS.gene011425:CDS
AAGCGGAACACAATGAGGCG+AGG 0.682806 8.2:+20926802 MS.gene011425:CDS
TTCCACTGGAGAGTCCGATG+AGG 0.697132 8.2:+20926895 MS.gene011425:CDS
TTTGATTCGATTGATCACAG+TGG 0.727954 8.2:+20926870 MS.gene011425:CDS
GACGAAGAAGAGGAGCATCG+CGG 0.735685 8.2:+20926054 MS.gene011425:CDS
TGTGAAGGAACATGAAACTG+TGG 0.739109 8.2:+20926659 MS.gene011425:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTTTGATGAATTTGAA+AGG + chr8.2:20925635-20925654 MS.gene011425:CDS 20.0%
!!! GAATTTTGATGAATTTGAAA+GGG + chr8.2:20925636-20925655 MS.gene011425:CDS 20.0%
! AACCATTTCATTGCAAAATT+GGG + chr8.2:20926144-20926163 MS.gene011425:CDS 25.0%
AAATAGAGCTAAGAGGAAAA+AGG + chr8.2:20925597-20925616 MS.gene011425:CDS 30.0%
AAATGAAGAAACTTGGGAAT+TGG - chr8.2:20926357-20926376 None:intergenic 30.0%
AATACATCTTCAACAGAAAC+TGG - chr8.2:20927085-20927104 None:intergenic 30.0%
AATAGAGCTAAGAGGAAAAA+GGG + chr8.2:20925598-20925617 MS.gene011425:CDS 30.0%
ACCATTTCATTGCAAAATTG+GGG + chr8.2:20926145-20926164 MS.gene011425:CDS 30.0%
ACTACTGGATGAAAATCAAA+GGG - chr8.2:20926603-20926622 None:intergenic 30.0%
CAACCATTTCATTGCAAAAT+TGG + chr8.2:20926143-20926162 MS.gene011425:CDS 30.0%
CATAACAACAATTGTAGGAT+TGG - chr8.2:20926772-20926791 None:intergenic 30.0%
CGGTAAAATAAGCACAAATT+CGG - chr8.2:20926203-20926222 None:intergenic 30.0%
GATGTGAAATGAAGAAACTT+GGG - chr8.2:20926363-20926382 None:intergenic 30.0%
! CAATCCTACAATTGTTGTTA+TGG + chr8.2:20926770-20926789 MS.gene011425:CDS 30.0%
! GTAACAAAGATCACCAATTT+TGG + chr8.2:20925853-20925872 MS.gene011425:CDS 30.0%
AATGCATCAGAACATGAAGA+GGG + chr8.2:20925973-20925992 MS.gene011425:CDS 35.0%
ACATAAAGCAAGGACTACAA+TGG + chr8.2:20926442-20926461 MS.gene011425:CDS 35.0%
AGTTTCAGTTTGGCACAATT+TGG + chr8.2:20925661-20925680 MS.gene011425:CDS 35.0%
ATAAGCACAAATTCGGCTTA+TGG - chr8.2:20926196-20926215 None:intergenic 35.0%
ATGCATTCTCATTCAACCTT+AGG - chr8.2:20925960-20925979 None:intergenic 35.0%
CAACATCATCATGTCAATGA+AGG + chr8.2:20925895-20925914 MS.gene011425:CDS 35.0%
CCAGAACAATTCAAAGAGAA+AGG - chr8.2:20925748-20925767 None:intergenic 35.0%
CCATTTCATTGCAAAATTGG+GGG + chr8.2:20926146-20926165 MS.gene011425:CDS 35.0%
CCTTTCTCTTTGAATTGTTC+TGG + chr8.2:20925745-20925764 MS.gene011425:CDS 35.0%
CGATGTGAAATGAAGAAACT+TGG - chr8.2:20926364-20926383 None:intergenic 35.0%
GAATTGGAGAAAGATGATAG+TGG + chr8.2:20927007-20927026 MS.gene011425:CDS 35.0%
GACTACTGGATGAAAATCAA+AGG - chr8.2:20926604-20926623 None:intergenic 35.0%
TAATTACAGTGTTCACAAGC+AGG + chr8.2:20927112-20927131 MS.gene011425:CDS 35.0%
TATTAAACCCTCCAAAACTG+TGG - chr8.2:20925708-20925727 None:intergenic 35.0%
TCAAAGAGAAAGGAATCGAA+GGG - chr8.2:20925738-20925757 None:intergenic 35.0%
TCTGCCATAACAACAATTGT+AGG - chr8.2:20926777-20926796 None:intergenic 35.0%
TGAAAGGGAAAGTTTCAGTT+TGG + chr8.2:20925651-20925670 MS.gene011425:CDS 35.0%
TTCAAAGAGAAAGGAATCGA+AGG - chr8.2:20925739-20925758 None:intergenic 35.0%
! CTTTTGCAAATAGAGCTAAG+AGG + chr8.2:20925590-20925609 MS.gene011425:CDS 35.0%
! GTTCCAAAGTTTAGCTTCAA+GGG + chr8.2:20926473-20926492 MS.gene011425:CDS 35.0%
! TGACATGATGATGTTGATGT+TGG - chr8.2:20925891-20925910 None:intergenic 35.0%
! TGTTCCAAAGTTTAGCTTCA+AGG + chr8.2:20926472-20926491 MS.gene011425:CDS 35.0%
! TTTGATTCGATTGATCACAG+TGG + chr8.2:20926870-20926889 MS.gene011425:CDS 35.0%
!! AAAGATCACCAATTTTGGTG+AGG + chr8.2:20925858-20925877 MS.gene011425:CDS 35.0%
!! AAGATCACCAATTTTGGTGA+GGG + chr8.2:20925859-20925878 MS.gene011425:CDS 35.0%
!! AATTTGGAGCTGGAAACTTT+TGG + chr8.2:20925677-20925696 MS.gene011425:CDS 35.0%
!! TACTTCTCGTGATTTTGACA+GGG + chr8.2:20926278-20926297 MS.gene011425:CDS 35.0%
!! TTTGAATTGTTCTGGAGATG+AGG + chr8.2:20925753-20925772 MS.gene011425:CDS 35.0%
AAGACACTTCATGATGGTAG+TGG + chr8.2:20926708-20926727 MS.gene011425:CDS 40.0%
AAGCAAGAGCTGAATGAAAC+CGG + chr8.2:20926540-20926559 MS.gene011425:CDS 40.0%
AATCTCTGTGTGCTCATTGA+TGG - chr8.2:20925316-20925335 None:intergenic 40.0%
AATGAAGGTTCAGTCTCCAA+TGG + chr8.2:20925910-20925929 MS.gene011425:CDS 40.0%
AGATAGAGGAGATGTTAGCT+AGG + chr8.2:20926922-20926941 MS.gene011425:CDS 40.0%
AGATGTTAGCTAGGGAGATA+AGG + chr8.2:20926931-20926950 MS.gene011425:CDS 40.0%
AGCCAAGGATTGTTAGACAA+AGG - chr8.2:20925823-20925842 None:intergenic 40.0%
ATCAATGAGCACACAGAGAT+TGG + chr8.2:20925315-20925334 MS.gene011425:CDS 40.0%
ATCGAATGAGATGCTGTTGA+GGG + chr8.2:20926380-20926399 MS.gene011425:CDS 40.0%
ATGAAACTGTCACTGCATTG+AGG + chr8.2:20926313-20926332 MS.gene011425:CDS 40.0%
CAAGAAGATACTGTGTAGAG+AGG - chr8.2:20927182-20927201 None:intergenic 40.0%
CTCTCTACACAGTATCTTCT+TGG + chr8.2:20927180-20927199 MS.gene011425:CDS 40.0%
CTGGATGAAAATCAAAGGGA+AGG - chr8.2:20926599-20926618 None:intergenic 40.0%
CTTCACAAGACACTTCATGA+TGG + chr8.2:20926702-20926721 MS.gene011425:CDS 40.0%
GAATGCATCAGAACATGAAG+AGG + chr8.2:20925972-20925991 MS.gene011425:CDS 40.0%
GACTTGAAGATGTAAGGCTT+TGG + chr8.2:20926628-20926647 MS.gene011425:CDS 40.0%
GATAGAGGAGATGTTAGCTA+GGG + chr8.2:20926923-20926942 MS.gene011425:CDS 40.0%
GCTAAGAGGAAAAAGGGTAA+TGG + chr8.2:20925604-20925623 MS.gene011425:CDS 40.0%
GGATTGTTAGACAAAGGCAA+AGG - chr8.2:20925817-20925836 None:intergenic 40.0%
TCACTCCTTAATCTGTGAGT+TGG - chr8.2:20925256-20925275 None:intergenic 40.0%
TCAGAACATGAAGAGGGTAA+TGG + chr8.2:20925979-20925998 MS.gene011425:CDS 40.0%
TCAGTTCCTTCATGCTGATA+AGG - chr8.2:20926015-20926034 None:intergenic 40.0%
TCCTCTATCTTGATCCTCAT+CGG - chr8.2:20926912-20926931 None:intergenic 40.0%
TCGACAGACTTGAAGATGTA+AGG + chr8.2:20926622-20926641 MS.gene011425:CDS 40.0%
TGCCTTTGTCTAACAATCCT+TGG + chr8.2:20925818-20925837 MS.gene011425:CDS 40.0%
TGTCACCAACTCACAGATTA+AGG + chr8.2:20925248-20925267 MS.gene011425:CDS 40.0%
TGTGAAGGAACATGAAACTG+TGG + chr8.2:20926659-20926678 MS.gene011425:CDS 40.0%
TTCTCCAATTCCCAAAACTC+TGG - chr8.2:20926998-20927017 None:intergenic 40.0%
! AAGAGGAAAAAGGGTAATGG+TGG + chr8.2:20925607-20925626 MS.gene011425:CDS 40.0%
! ACATGAAGAGGGTAATGGTT+CGG + chr8.2:20925984-20926003 MS.gene011425:CDS 40.0%
! ACTGGTTGTGTTGATGCTAT+TGG + chr8.2:20926099-20926118 MS.gene011425:CDS 40.0%
! AGATCAAGGATGAGGAGTTT+TGG + chr8.2:20925512-20925531 MS.gene011425:CDS 40.0%
! ATCGACTTCGACATAAAGCA+AGG + chr8.2:20926432-20926451 MS.gene011425:CDS 40.0%
! CATGATGATGTTGATGTTGG+TGG - chr8.2:20925888-20925907 None:intergenic 40.0%
! CCCCCAATTTTGCAATGAAA+TGG - chr8.2:20926149-20926168 None:intergenic 40.0%
! CTGGTTGTGTTGATGCTATT+GGG + chr8.2:20926100-20926119 MS.gene011425:CDS 40.0%
! GATCAAGGATGAGGAGTTTT+GGG + chr8.2:20925513-20925532 MS.gene011425:CDS 40.0%
! TTTTGGTCTGTCCACAGTTT+TGG + chr8.2:20925694-20925713 MS.gene011425:CDS 40.0%
!! AGAAAGATGATAGTGGAGCA+TGG + chr8.2:20927014-20927033 MS.gene011425:CDS 40.0%
!! ATTGTTCTGGAGATGAGGAA+AGG + chr8.2:20925758-20925777 MS.gene011425:CDS 40.0%
!! CTACTTCTCGTGATTTTGAC+AGG + chr8.2:20926277-20926296 MS.gene011425:CDS 40.0%
!! GCGCTGATTTGAAAAACATG+AGG - chr8.2:20926258-20926277 None:intergenic 40.0%
!! GCTAAACTTTGGAACAAGCT+AGG - chr8.2:20926468-20926487 None:intergenic 40.0%
!! GGCTTTTGAAGGGAAAGAAA+GGG + chr8.2:20926401-20926420 MS.gene011425:CDS 40.0%
!! TTGTTCTGGAGATGAGGAAA+GGG + chr8.2:20925759-20925778 MS.gene011425:CDS 40.0%
!!! AGGAGCATTGAGAGACTTTT+TGG + chr8.2:20926731-20926750 MS.gene011425:CDS 40.0%
!!! GGAGCATTGAGAGACTTTTT+GGG + chr8.2:20926732-20926751 MS.gene011425:CDS 40.0%
AAAACCAGCAAGTCTGTCTC+CGG - chr8.2:20926562-20926581 None:intergenic 45.0%
AAAGGGAAGGTTCAATGCCT+CGG - chr8.2:20926586-20926605 None:intergenic 45.0%
ACTTGGGAATTGGTGTAACC+TGG - chr8.2:20926347-20926366 None:intergenic 45.0%
AGAGTCAGAGTTTGAGCCTA+AGG + chr8.2:20925941-20925960 MS.gene011425:CDS 45.0%
AGCAACAACTGCTCAGACAT+TGG + chr8.2:20925486-20925505 MS.gene011425:CDS 45.0%
AGGAGCAACAGCACTTACTT+TGG + chr8.2:20927142-20927161 MS.gene011425:CDS 45.0%
ATGCATGGGAGTCACTGATA+GGG + chr8.2:20927046-20927065 MS.gene011425:CDS 45.0%
ATTGGGAGAGATCAAGGATG+AGG + chr8.2:20925504-20925523 MS.gene011425:CDS 45.0%
CAACAGCACTTACTTTGGGT+TGG + chr8.2:20927147-20927166 MS.gene011425:CDS 45.0%
CACAGTATCTTCTTGGACAC+CGG + chr8.2:20927187-20927206 MS.gene011425:CDS 45.0%
CATCGAATGAGATGCTGTTG+AGG + chr8.2:20926379-20926398 MS.gene011425:CDS 45.0%
CTGACTCTCTGATGAACCAT+TGG - chr8.2:20925929-20925948 None:intergenic 45.0%
GCAACAACTGCTCAGACATT+GGG + chr8.2:20925487-20925506 MS.gene011425:CDS 45.0%
GCTTGCAGAACATGGATCTT+TGG + chr8.2:20925561-20925580 MS.gene011425:CDS 45.0%
GGAGCAACAGCACTTACTTT+GGG + chr8.2:20927143-20927162 MS.gene011425:CDS 45.0%
GGGAATCCTTATCAGCATGA+AGG + chr8.2:20926006-20926025 MS.gene011425:CDS 45.0%
GTCTGAGCAGTTGTTGCTAA+AGG - chr8.2:20925484-20925503 None:intergenic 45.0%
GTTACAGCAGATTCAAGCCA+AGG - chr8.2:20925838-20925857 None:intergenic 45.0%
GTTCTCTGCAGCAGAACATT+CGG + chr8.2:20925452-20925471 MS.gene011425:CDS 45.0%
TCAAGCTGGCTGAGAAAAAG+AGG - chr8.2:20927234-20927253 None:intergenic 45.0%
TCAGACATTGGGAGAGATCA+AGG + chr8.2:20925498-20925517 MS.gene011425:CDS 45.0%
TCAGCATGAAGGAACTGAAG+TGG + chr8.2:20926017-20926036 MS.gene011425:CDS 45.0%
TCCAAGCCATGTTCGAATCA+CGG + chr8.2:20926503-20926522 MS.gene011425:CDS 45.0%
TCCGATGAGGATCAAGATAG+AGG + chr8.2:20926908-20926927 MS.gene011425:CDS 45.0%
TCTTTACGAGGGAAGCTACA+AGG - chr8.2:20925346-20925365 None:intergenic 45.0%
TGAGCAGTTGTTGCTAAAGG+TGG - chr8.2:20925481-20925500 None:intergenic 45.0%
TGCATTGAGGCTTCTAAACC+AGG + chr8.2:20926326-20926345 MS.gene011425:CDS 45.0%
TGCTATTGGGTCAAGGAATG+TGG + chr8.2:20926113-20926132 MS.gene011425:CDS 45.0%
TGGCCCTTGAAGCTAAACTT+TGG - chr8.2:20926479-20926498 None:intergenic 45.0%
TGTAGCTTCCCTCGTAAAGA+CGG + chr8.2:20925346-20925365 MS.gene011425:CDS 45.0%
TGTTATGGCAGAGCAAGAAG+CGG + chr8.2:20926785-20926804 MS.gene011425:CDS 45.0%
TTCTCAACTGAAATCCCCAC+CGG - chr8.2:20925400-20925419 None:intergenic 45.0%
TTGAAGAGGCTTGCAGAACA+TGG + chr8.2:20925553-20925572 MS.gene011425:CDS 45.0%
! AGTTTGGCACAATTTGGAGC+TGG + chr8.2:20925667-20925686 MS.gene011425:CDS 45.0%
! ATGAAGAGGGTAATGGTTCG+GGG + chr8.2:20925986-20926005 MS.gene011425:CDS 45.0%
! ATGCTGTTGAGGGCTTTTGA+AGG + chr8.2:20926390-20926409 MS.gene011425:CDS 45.0%
! CATGAAGAGGGTAATGGTTC+GGG + chr8.2:20925985-20926004 MS.gene011425:CDS 45.0%
! GCTTTGGATGCTTCATGTGA+AGG + chr8.2:20926644-20926663 MS.gene011425:CDS 45.0%
! GTGTTGATGCTATTGGGTCA+AGG + chr8.2:20926106-20926125 MS.gene011425:CDS 45.0%
! TCTTCAAGTCTGTCGACTAC+TGG - chr8.2:20926618-20926637 None:intergenic 45.0%
! TGCTGTTGAGGGCTTTTGAA+GGG + chr8.2:20926391-20926410 MS.gene011425:CDS 45.0%
!! AAGATGATAGTGGAGCATGG+AGG + chr8.2:20927017-20927036 MS.gene011425:CDS 45.0%
!! ACACTTCATGATGGTAGTGG+AGG + chr8.2:20926711-20926730 MS.gene011425:CDS 45.0%
!! AGATGATAGTGGAGCATGGA+GGG + chr8.2:20927018-20927037 MS.gene011425:CDS 45.0%
!! GAGCTTTTGAGCTTGCTTAC+TGG + chr8.2:20926081-20926100 MS.gene011425:CDS 45.0%
!! GGGCTTTTGAAGGGAAAGAA+AGG + chr8.2:20926400-20926419 MS.gene011425:CDS 45.0%
!! TAGGATTGGTGCTACGAATG+AGG - chr8.2:20926758-20926777 None:intergenic 45.0%
!! TTCTCGTGATTTTGACAGGG+TGG + chr8.2:20926281-20926300 MS.gene011425:CDS 45.0%
!!! GATGAGGAGTTTTGGGAGAA+GGG + chr8.2:20925520-20925539 MS.gene011425:CDS 45.0%
ACATCGTTGCTTGCGAAGGA+AGG + chr8.2:20926955-20926974 MS.gene011425:CDS 50.0%
AGGAACATCGTTGCTTGCGA+AGG + chr8.2:20926951-20926970 MS.gene011425:CDS 50.0%
AGGAGAAGAGGACGAAGAAG+AGG + chr8.2:20926044-20926063 MS.gene011425:CDS 50.0%
ATGTTCGAATCACGGGTGTG+GGG + chr8.2:20926511-20926530 MS.gene011425:CDS 50.0%
CATCGTTGCTTGCGAAGGAA+GGG + chr8.2:20926956-20926975 MS.gene011425:CDS 50.0%
CATGGAGGGTTCAGATGCAT+GGG + chr8.2:20927032-20927051 MS.gene011425:CDS 50.0%
CATGTTCGAATCACGGGTGT+GGG + chr8.2:20926510-20926529 MS.gene011425:CDS 50.0%
CCAAAGCCTAGTGACTCTGA+TGG - chr8.2:20926235-20926254 None:intergenic 50.0%
CCAAGCCATGTTCGAATCAC+GGG + chr8.2:20926504-20926523 MS.gene011425:CDS 50.0%
CCATCAGAGTCACTAGGCTT+TGG + chr8.2:20926232-20926251 MS.gene011425:CDS 50.0%
CCTAGTGACTCTGATGGCTA+AGG - chr8.2:20926229-20926248 None:intergenic 50.0%
CCTTAGCCATCAGAGTCACT+AGG + chr8.2:20926226-20926245 MS.gene011425:CDS 50.0%
CTGCTGCTGTTCTTGACTTC+AGG - chr8.2:20925424-20925443 None:intergenic 50.0%
GAAGGAACTGAAGTGGAGAC+AGG + chr8.2:20926024-20926043 MS.gene011425:CDS 50.0%
GACTCTGATGGCTAAGGCTT+CGG - chr8.2:20926223-20926242 None:intergenic 50.0%
GATGCATGGGAGTCACTGAT+AGG + chr8.2:20927045-20927064 MS.gene011425:CDS 50.0%
GCAAGAAGCGGAACACAATG+AGG + chr8.2:20926797-20926816 MS.gene011425:CDS 50.0%
GCGAAGAACCGTCTTTACGA+GGG - chr8.2:20925357-20925376 None:intergenic 50.0%
GGTGCAGAAATCACTTCAGC+AGG - chr8.2:20925796-20925815 None:intergenic 50.0%
GTGGCTACCCTCACCAAAAT+TGG - chr8.2:20925869-20925888 None:intergenic 50.0%
TGAAGTGGAGACAGGAGAAG+AGG + chr8.2:20926032-20926051 MS.gene011425:CDS 50.0%
! GTGATTCGAACATGGCTTGG+AGG - chr8.2:20926504-20926523 None:intergenic 50.0%
! TCGAACATGGCTTGGAGGAT+TGG - chr8.2:20926499-20926518 None:intergenic 50.0%
!! TCGTGATTTTGACAGGGTGG+TGG + chr8.2:20926284-20926303 MS.gene011425:CDS 50.0%
!! TGGTCTGTCCACAGTTTTGG+AGG + chr8.2:20925697-20925716 MS.gene011425:CDS 50.0%
!!! AGGAGTTTTGGGAGAAGGGT+GGG + chr8.2:20925524-20925543 MS.gene011425:CDS 50.0%
!!! GGATGAGGAGTTTTGGGAGA+AGG + chr8.2:20925519-20925538 MS.gene011425:CDS 50.0%
!!! GGCACCAGAGTTTTGGGAAT+TGG + chr8.2:20926991-20927010 MS.gene011425:CDS 50.0%
!!! GGTCTGTCCACAGTTTTGGA+GGG + chr8.2:20925698-20925717 MS.gene011425:CDS 50.0%
!!! TGGAGAGGCACCAGAGTTTT+GGG + chr8.2:20926985-20927004 MS.gene011425:CDS 50.0%
!!! TTGGAGAGGCACCAGAGTTT+TGG + chr8.2:20926984-20927003 MS.gene011425:CDS 50.0%
AAGCGGAACACAATGAGGCG+AGG + chr8.2:20926802-20926821 MS.gene011425:CDS 55.0%
AGGGTGGGAGGAGTTTGAAG+AGG + chr8.2:20925539-20925558 MS.gene011425:CDS 55.0%
ATCCTCATCGGACTCTCCAG+TGG - chr8.2:20926900-20926919 None:intergenic 55.0%
CAGTGTTCACAAGCAGGAGC+AGG + chr8.2:20927118-20927137 MS.gene011425:CDS 55.0%
CCACACCCGTGATTCGAACA+TGG - chr8.2:20926512-20926531 None:intergenic 55.0%
CCATGTTCGAATCACGGGTG+TGG + chr8.2:20926509-20926528 MS.gene011425:CDS 55.0%
CCCGTGATTCGAACATGGCT+TGG - chr8.2:20926507-20926526 None:intergenic 55.0%
CGCGAAGAACCGTCTTTACG+AGG - chr8.2:20925358-20925377 None:intergenic 55.0%
CGGCTTATGGAAGTTCCCCT+TGG - chr8.2:20926183-20926202 None:intergenic 55.0%
GAAACCGGAGACAGACTTGC+TGG + chr8.2:20926555-20926574 MS.gene011425:CDS 55.0%
GAGCTTCCAGCAACGTCAAC+CGG - chr8.2:20927209-20927228 None:intergenic 55.0%
GCATGGAGGGTTCAGATGCA+TGG + chr8.2:20927031-20927050 MS.gene011425:CDS 55.0%
GGAACACAATGAGGCGAGGT+TGG + chr8.2:20926806-20926825 MS.gene011425:CDS 55.0%
GGGGATCTTGCATCTCCAAG+GGG + chr8.2:20926165-20926184 MS.gene011425:CDS 55.0%
GGGGGATCTTGCATCTCCAA+GGG + chr8.2:20926164-20926183 MS.gene011425:CDS 55.0%
GTTCACAAGCAGGAGCAGGA+AGG + chr8.2:20927122-20927141 MS.gene011425:CDS 55.0%
TCATCGGACTCTCCAGTGGA+AGG - chr8.2:20926896-20926915 None:intergenic 55.0%
TGATCACAGTGGCCTTCCAC+TGG + chr8.2:20926881-20926900 MS.gene011425:CDS 55.0%
TGGGGGATCTTGCATCTCCA+AGG + chr8.2:20926163-20926182 MS.gene011425:CDS 55.0%
TGTTCGAATCACGGGTGTGG+GGG + chr8.2:20926512-20926531 MS.gene011425:CDS 55.0%
TTCCACTGGAGAGTCCGATG+AGG + chr8.2:20926895-20926914 MS.gene011425:CDS 55.0%
TTGCGAAGGAAGGGACAGGT+TGG + chr8.2:20926965-20926984 MS.gene011425:CDS 55.0%
TTGCTTGCGAAGGAAGGGAC+AGG + chr8.2:20926961-20926980 MS.gene011425:CDS 55.0%
! AAGGAAGGGACAGGTTGGAG+AGG + chr8.2:20926970-20926989 MS.gene011425:CDS 55.0%
!! GACGAAGAAGAGGAGCATCG+CGG + chr8.2:20926054-20926073 MS.gene011425:CDS 55.0%
!!! AGTTTTGGGAGAAGGGTGGG+AGG + chr8.2:20925527-20925546 MS.gene011425:CDS 55.0%
!!! CAGACTTGCTGGTTTTGCCG+AGG + chr8.2:20926566-20926585 MS.gene011425:CDS 55.0%
!!! GAGGAGTTTTGGGAGAAGGG+TGG + chr8.2:20925523-20925542 MS.gene011425:CDS 55.0%
GAAATCCCCACCGGCCTCAA+CGG - chr8.2:20925391-20925410 None:intergenic 60.0%
! TGGACACCGGTTGACGTTGC+TGG + chr8.2:20927200-20927219 MS.gene011425:CDS 60.0%
TTCGCAGCAGCCGTTGAGGC+CGG + chr8.2:20925378-20925397 MS.gene011425:CDS 65.0%
AGCAGCCGTTGAGGCCGGTG+GGG + chr8.2:20925383-20925402 MS.gene011425:CDS 70.0%
CAGCAGCCGTTGAGGCCGGT+GGG + chr8.2:20925382-20925401 MS.gene011425:CDS 70.0%
CGCGTTCGCAGCAGCCGTTG+AGG + chr8.2:20925374-20925393 MS.gene011425:CDS 70.0%
GCAGCAGCCGTTGAGGCCGG+TGG + chr8.2:20925381-20925400 MS.gene011425:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 20925217 20927253 20925217 ID=MS.gene011425
chr8.2 mRNA 20925217 20927253 20925217 ID=MS.gene011425.t1;Parent=MS.gene011425
chr8.2 exon 20925217 20927253 20925217 ID=MS.gene011425.t1.exon1;Parent=MS.gene011425.t1
chr8.2 CDS 20925217 20927253 20925217 ID=cds.MS.gene011425.t1;Parent=MS.gene011425.t1
Gene Sequence

>MS.gene011425

ATGTTGGCTGGTTGTTCTAGCTCGACATTGTTGTCACCAACTCACAGATTAAGGAGTGAAGCATCAGCACAGTTTCAAGCTTGTCATTTTCAGTTACCATCAATGAGCACACAGAGATTGGACTTACCTTGTAGCTTCCCTCGTAAAGACGGTTCTTCGCGTTCGCAGCAGCCGTTGAGGCCGGTGGGGATTTCAGTTGAGAAGCCTGAAGTCAAGAACAGCAGCAGCAGTACTTGTTCTCTGCAGCAGAACATTCGGTTACCACCTTTAGCAACAACTGCTCAGACATTGGGAGAGATCAAGGATGAGGAGTTTTGGGAGAAGGGTGGGAGGAGTTTGAAGAGGCTTGCAGAACATGGATCTTTGGATGAGTCTTTTGCAAATAGAGCTAAGAGGAAAAAGGGTAATGGTGGTGATGAGAATTTTGATGAATTTGAAAGGGAAAGTTTCAGTTTGGCACAATTTGGAGCTGGAAACTTTTGGTCTGTCCACAGTTTTGGAGGGTTTAATAGTAATAGCCCTTCGATTCCTTTCTCTTTGAATTGTTCTGGAGATGAGGAAAGGGTTTGTTTTGCTCCTGCTGAAGTGATTTCTGCACCTTTGCCTTTGTCTAACAATCCTTGGCTTGAATCTGCTGTAACAAAGATCACCAATTTTGGTGAGGGTAGCCACCAACATCAACATCATCATGTCAATGAAGGTTCAGTCTCCAATGGTTCATCAGAGAGTCAGAGTTTGAGCCTAAGGTTGAATGAGAATGCATCAGAACATGAAGAGGGTAATGGTTCGGGGAATCCTTATCAGCATGAAGGAACTGAAGTGGAGACAGGAGAAGAGGACGAAGAAGAGGAGCATCGCGGTTTTGAGCTTTTGAGCTTGCTTACTGGTTGTGTTGATGCTATTGGGTCAAGGAATGTGGCTGCAGTCAACCATTTCATTGCAAAATTGGGGGATCTTGCATCTCCAAGGGGAACTTCCATAAGCCGAATTTGTGCTTATTTTACCGAAGCCTTAGCCATCAGAGTCACTAGGCTTTGGCCTCATGTTTTTCAAATCAGCGCTACTTCTCGTGATTTTGACAGGGTGGTGGATGATGATGAAACTGTCACTGCATTGAGGCTTCTAAACCAGGTTACACCAATTCCCAAGTTTCTTCATTTCACATCGAATGAGATGCTGTTGAGGGCTTTTGAAGGGAAAGAAAGGGTTCACATTATCGACTTCGACATAAAGCAAGGACTACAATGGCCTAGCTTGTTCCAAAGTTTAGCTTCAAGGGCCAATCCTCCAAGCCATGTTCGAATCACGGGTGTGGGGGAATCGAAGCAAGAGCTGAATGAAACCGGAGACAGACTTGCTGGTTTTGCCGAGGCATTGAACCTTCCCTTTGATTTTCATCCAGTAGTCGACAGACTTGAAGATGTAAGGCTTTGGATGCTTCATGTGAAGGAACATGAAACTGTGGCTGTGAACTGTGTTTTGCAGCTTCACAAGACACTTCATGATGGTAGTGGAGGAGCATTGAGAGACTTTTTGGGCCTCATTCGTAGCACCAATCCTACAATTGTTGTTATGGCAGAGCAAGAAGCGGAACACAATGAGGCGAGGTTGGACTCAAGAGTCTGCAATTCATTGAAATATTACTCAGCGCTGTTTGATTCGATTGATCACAGTGGCCTTCCACTGGAGAGTCCGATGAGGATCAAGATAGAGGAGATGTTAGCTAGGGAGATAAGGAACATCGTTGCTTGCGAAGGAAGGGACAGGTTGGAGAGGCACCAGAGTTTTGGGAATTGGAGAAAGATGATAGTGGAGCATGGAGGGTTCAGATGCATGGGAGTCACTGATAGGGAACTGATTCAGAGCCAGTTTCTGTTGAAGATGTATTCTTGTGATAATTACAGTGTTCACAAGCAGGAGCAGGAAGGAGCAACAGCACTTACTTTGGGTTGGCTTGATCAACCTCTCTACACAGTATCTTCTTGGACACCGGTTGACGTTGCTGGAAGCTCATCCTCTTTTTCTCAGCCAGCTTGA
Protein sequence

>MS.gene011425.t1

MLAGCSSSTLLSPTHRLRSEASAQFQACHFQLPSMSTQRLDLPCSFPRKDGSSRSQQPLRPVGISVEKPEVKNSSSSTCSLQQNIRLPPLATTAQTLGEIKDEEFWEKGGRSLKRLAEHGSLDESFANRAKRKKGNGGDENFDEFERESFSLAQFGAGNFWSVHSFGGFNSNSPSIPFSLNCSGDEERVCFAPAEVISAPLPLSNNPWLESAVTKITNFGEGSHQHQHHHVNEGSVSNGSSESQSLSLRLNENASEHEEGNGSGNPYQHEGTEVETGEEDEEEEHRGFELLSLLTGCVDAIGSRNVAAVNHFIAKLGDLASPRGTSISRICAYFTEALAIRVTRLWPHVFQISATSRDFDRVVDDDETVTALRLLNQVTPIPKFLHFTSNEMLLRAFEGKERVHIIDFDIKQGLQWPSLFQSLASRANPPSHVRITGVGESKQELNETGDRLAGFAEALNLPFDFHPVVDRLEDVRLWMLHVKEHETVAVNCVLQLHKTLHDGSGGALRDFLGLIRSTNPTIVVMAEQEAEHNEARLDSRVCNSLKYYSALFDSIDHSGLPLESPMRIKIEEMLAREIRNIVACEGRDRLERHQSFGNWRKMIVEHGGFRCMGVTDRELIQSQFLLKMYSCDNYSVHKQEQEGATALTLGWLDQPLYTVSSWTPVDVAGSSSSFSQPA