Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011536.t1 | XP_003608704.1 | 88.4 | 320 | 32 | 3 | 1 | 320 | 1 | 315 | 6.90E-108 | 400.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011536.t1 | Q9M202 | 31.1 | 328 | 173 | 7 | 1 | 317 | 1 | 286 | 7.3e-34 | 146.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011536.t1 | G7JIV8 | 88.4 | 320 | 32 | 3 | 1 | 320 | 1 | 315 | 5.0e-108 | 400.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene011536.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011536.t1 | MTR_4g100860 | 94.375 | 320 | 13 | 3 | 1 | 320 | 1 | 315 | 0.0 | 582 |
| MS.gene011536.t1 | MTR_0160s0030 | 42.234 | 367 | 153 | 13 | 1 | 320 | 1 | 355 | 2.02e-66 | 213 |
| MS.gene011536.t1 | MTR_5g012630 | 42.724 | 323 | 103 | 14 | 1 | 305 | 1 | 259 | 7.99e-59 | 190 |
| MS.gene011536.t1 | MTR_4g027440 | 34.332 | 367 | 160 | 8 | 1 | 308 | 2 | 346 | 2.79e-43 | 153 |
| MS.gene011536.t1 | MTR_4g109340 | 29.167 | 216 | 85 | 8 | 3 | 176 | 10 | 199 | 2.30e-11 | 64.7 |
| MS.gene011536.t1 | MTR_4g082980 | 50.000 | 60 | 28 | 1 | 251 | 310 | 393 | 450 | 5.72e-11 | 63.5 |
| MS.gene011536.t1 | MTR_1g018420 | 27.919 | 197 | 103 | 6 | 132 | 306 | 14 | 193 | 6.49e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011536.t1 | AT3G60580 | 36.145 | 332 | 151 | 11 | 1 | 317 | 1 | 286 | 2.85e-46 | 158 |
| MS.gene011536.t1 | AT1G02030 | 35.843 | 332 | 129 | 11 | 2 | 317 | 3 | 266 | 1.93e-41 | 145 |
| MS.gene011536.t1 | AT2G45120 | 35.103 | 339 | 173 | 10 | 1 | 319 | 1 | 312 | 4.84e-39 | 140 |
| MS.gene011536.t1 | AT4G16610 | 38.372 | 172 | 85 | 4 | 3 | 173 | 4 | 155 | 1.63e-24 | 99.0 |
| MS.gene011536.t1 | AT1G26610 | 45.205 | 73 | 37 | 1 | 238 | 310 | 386 | 455 | 1.91e-12 | 68.2 |
| MS.gene011536.t1 | AT3G53600 | 36.364 | 110 | 55 | 5 | 213 | 311 | 51 | 156 | 3.16e-11 | 61.6 |
| MS.gene011536.t1 | AT5G61470 | 43.902 | 82 | 36 | 3 | 205 | 276 | 218 | 299 | 3.33e-11 | 63.5 |
Find 48 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTGTTGTAGAAAATTC+TGG | 0.230051 | 8.2:+22201323 | None:intergenic |
| CCAAACACCTTAAAACAAAA+TGG | 0.311153 | 8.2:+22200932 | None:intergenic |
| TCACGTACAAAAGAAAATTC+AGG | 0.344635 | 8.2:+22201463 | None:intergenic |
| CACATAGCAACATCTTCTTC+AGG | 0.374474 | 8.2:+22201217 | None:intergenic |
| GGCACCATGAAACTCGGCTT+TGG | 0.378686 | 8.2:+22201298 | None:intergenic |
| TCTTTGAATCTAGCAGCAAT+AGG | 0.389886 | 8.2:+22200824 | None:intergenic |
| GAAACCAAAGCCGAGTTTCA+TGG | 0.392923 | 8.2:-22201302 | MS.gene011536:CDS |
| AAGCTTTGTTCAAGGACGTT+TGG | 0.415966 | 8.2:-22201673 | MS.gene011536:CDS |
| TCCGATGATGAGAAAGAATC+AGG | 0.418255 | 8.2:+22201574 | None:intergenic |
| TAGATCGTTGTTGACGAGTC+GGG | 0.431866 | 8.2:+22201386 | None:intergenic |
| TGATGCTTTCGCGAGACAAA+TGG | 0.443203 | 8.2:-22201189 | MS.gene011536:CDS |
| ACCTGATTCTTTCTCATCAT+CGG | 0.450240 | 8.2:-22201575 | MS.gene011536:CDS |
| TTGTTGTCGTCGTTGTTGTT+TGG | 0.451043 | 8.2:+22201349 | None:intergenic |
| GTGAATTGTGATGGAGTTGT+TGG | 0.452696 | 8.2:+22201277 | None:intergenic |
| GTTTCGATCTTCAAGAGCAT+TGG | 0.459910 | 8.2:-22201035 | MS.gene011536:CDS |
| TTTCGATCTTCAAGAGCATT+GGG | 0.469548 | 8.2:-22201034 | MS.gene011536:CDS |
| TTTGGTTCTGGTCAAGCACT+TGG | 0.469573 | 8.2:-22200914 | MS.gene011536:CDS |
| CTCGTCAACAACGATCTAAA+CGG | 0.483916 | 8.2:-22201381 | MS.gene011536:CDS |
| GGTTCTGGTCAAGCACTTGG+TGG | 0.484093 | 8.2:-22200911 | MS.gene011536:CDS |
| TGAAGAAGAAGAGGGATCGG+TGG | 0.488736 | 8.2:-22201128 | MS.gene011536:CDS |
| TCATCTTCTTCAAGTGGAGC+TGG | 0.500458 | 8.2:+22200779 | None:intergenic |
| ACAAATGCAAGCTTTGTTCA+AGG | 0.502681 | 8.2:-22201681 | MS.gene011536:CDS |
| GCGAAGAATGGTAATGGTAA+TGG | 0.505680 | 8.2:-22200980 | MS.gene011536:CDS |
| ACATAGCAACATCTTCTTCA+GGG | 0.525812 | 8.2:+22201218 | None:intergenic |
| AAGTTATTGAAACGAGTTCG+TGG | 0.531632 | 8.2:-22201088 | MS.gene011536:CDS |
| GCAGTGGAAGTTGATGAAGA+AGG | 0.533864 | 8.2:+22200863 | None:intergenic |
| TGTCGCGATGAAGCGAAGAA+TGG | 0.548591 | 8.2:-22200992 | MS.gene011536:CDS |
| TCTTGTTCAGTGAATTGTGA+TGG | 0.549593 | 8.2:+22201268 | None:intergenic |
| CAGTGGAAGTTGATGAAGAA+GGG | 0.550853 | 8.2:+22200864 | None:intergenic |
| TTTGGCAATGGAAGAGCACT+TGG | 0.550999 | 8.2:-22201655 | MS.gene011536:CDS |
| GTTGTTGGCACCATGAAACT+CGG | 0.554243 | 8.2:+22201292 | None:intergenic |
| TTAGATCGTTGTTGACGAGT+CGG | 0.558622 | 8.2:+22201385 | None:intergenic |
| GGAACAAGATGAAGAAGAAG+AGG | 0.558902 | 8.2:-22201137 | MS.gene011536:CDS |
| AGATCATCATCTTCTTCAAG+TGG | 0.559232 | 8.2:+22200773 | None:intergenic |
| GGCAATGGAAGAGCACTTGG+TGG | 0.563472 | 8.2:-22201652 | MS.gene011536:CDS |
| TGTTCAAGGACGTTTGGCAA+TGG | 0.572618 | 8.2:-22201667 | MS.gene011536:CDS |
| GATGAAGCGAAGAATGGTAA+TGG | 0.574456 | 8.2:-22200986 | MS.gene011536:CDS |
| ATTAACATTAACATTAGCAG+TGG | 0.581335 | 8.2:+22200847 | None:intergenic |
| AGCACTTGGTGGACACATGA+AGG | 0.581864 | 8.2:-22201641 | MS.gene011536:CDS |
| AGATGAAGAAGAAGAGGGAT+CGG | 0.594748 | 8.2:-22201131 | MS.gene011536:CDS |
| AGTTATTGAAACGAGTTCGT+GGG | 0.596995 | 8.2:-22201087 | MS.gene011536:CDS |
| ATATCAGAAACTGAACTCAC+AGG | 0.618063 | 8.2:+22201244 | None:intergenic |
| CATAGCAACATCTTCTTCAG+GGG | 0.618873 | 8.2:+22201219 | None:intergenic |
| GAACAAGATGAAGAAGAAGA+GGG | 0.633530 | 8.2:-22201136 | MS.gene011536:CDS |
| AGCATTTGTGTGAAAACTGT+GGG | 0.639279 | 8.2:-22201063 | MS.gene011536:CDS |
| ATCGGTGGAGAAAATATCGA+AGG | 0.639870 | 8.2:-22201113 | MS.gene011536:CDS |
| GATGAACAACATCAACAATG+TGG | 0.658258 | 8.2:-22201158 | MS.gene011536:CDS |
| AAGCATTTGTGTGAAAACTG+TGG | 0.714401 | 8.2:-22201064 | MS.gene011536:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAACATTAACATTAGCAG+TGG | + | chr8.2:22201598-22201617 | None:intergenic | 25.0% |
| !!! | TGAATGTCCATTTTGTTTTA+AGG | - | chr8.2:22201503-22201522 | MS.gene011536:CDS | 25.0% |
| CCAAACACCTTAAAACAAAA+TGG | + | chr8.2:22201513-22201532 | None:intergenic | 30.0% | |
| TCACGTACAAAAGAAAATTC+AGG | + | chr8.2:22200982-22201001 | None:intergenic | 30.0% | |
| TCTTCTGTTGTAGAAAATTC+TGG | + | chr8.2:22201122-22201141 | None:intergenic | 30.0% | |
| !!! | CCATTTTGTTTTAAGGTGTT+TGG | - | chr8.2:22201510-22201529 | MS.gene011536:CDS | 30.0% |
| AAGCATTTGTGTGAAAACTG+TGG | - | chr8.2:22201378-22201397 | MS.gene011536:CDS | 35.0% | |
| AAGTTATTGAAACGAGTTCG+TGG | - | chr8.2:22201354-22201373 | MS.gene011536:CDS | 35.0% | |
| ACATAGCAACATCTTCTTCA+GGG | + | chr8.2:22201227-22201246 | None:intergenic | 35.0% | |
| ACCTGATTCTTTCTCATCAT+CGG | - | chr8.2:22200867-22200886 | MS.gene011536:CDS | 35.0% | |
| AGATCATCATCTTCTTCAAG+TGG | + | chr8.2:22201672-22201691 | None:intergenic | 35.0% | |
| AGCATTTGTGTGAAAACTGT+GGG | - | chr8.2:22201379-22201398 | MS.gene011536:CDS | 35.0% | |
| AGTTATTGAAACGAGTTCGT+GGG | - | chr8.2:22201355-22201374 | MS.gene011536:CDS | 35.0% | |
| ATATCAGAAACTGAACTCAC+AGG | + | chr8.2:22201201-22201220 | None:intergenic | 35.0% | |
| GAACAAGATGAAGAAGAAGA+GGG | - | chr8.2:22201306-22201325 | MS.gene011536:CDS | 35.0% | |
| GATGAACAACATCAACAATG+TGG | - | chr8.2:22201284-22201303 | MS.gene011536:CDS | 35.0% | |
| TCTTGTTCAGTGAATTGTGA+TGG | + | chr8.2:22201177-22201196 | None:intergenic | 35.0% | |
| TCTTTGAATCTAGCAGCAAT+AGG | + | chr8.2:22201621-22201640 | None:intergenic | 35.0% | |
| !! | ACAAATGCAAGCTTTGTTCA+AGG | - | chr8.2:22200761-22200780 | MS.gene011536:CDS | 35.0% |
| !! | TTTCGATCTTCAAGAGCATT+GGG | - | chr8.2:22201408-22201427 | MS.gene011536:CDS | 35.0% |
| !!! | GCAACTTTGAGACTTTTCTT+GGG | + | chr8.2:22200955-22200974 | None:intergenic | 35.0% |
| !!! | TGCAACTTTGAGACTTTTCT+TGG | + | chr8.2:22200956-22200975 | None:intergenic | 35.0% |
| !!! | TGTTTTAAGGTGTTTGGTTC+TGG | - | chr8.2:22201516-22201535 | MS.gene011536:CDS | 35.0% |
| AGATGAAGAAGAAGAGGGAT+CGG | - | chr8.2:22201311-22201330 | MS.gene011536:CDS | 40.0% | |
| ATCGGTGGAGAAAATATCGA+AGG | - | chr8.2:22201329-22201348 | MS.gene011536:CDS | 40.0% | |
| CACATAGCAACATCTTCTTC+AGG | + | chr8.2:22201228-22201247 | None:intergenic | 40.0% | |
| CATAGCAACATCTTCTTCAG+GGG | + | chr8.2:22201226-22201245 | None:intergenic | 40.0% | |
| CTCGTCAACAACGATCTAAA+CGG | - | chr8.2:22201061-22201080 | MS.gene011536:CDS | 40.0% | |
| GATGAAGCGAAGAATGGTAA+TGG | - | chr8.2:22201456-22201475 | MS.gene011536:CDS | 40.0% | |
| GGAACAAGATGAAGAAGAAG+AGG | - | chr8.2:22201305-22201324 | MS.gene011536:CDS | 40.0% | |
| TCCGATGATGAGAAAGAATC+AGG | + | chr8.2:22200871-22200890 | None:intergenic | 40.0% | |
| TTAGATCGTTGTTGACGAGT+CGG | + | chr8.2:22201060-22201079 | None:intergenic | 40.0% | |
| ! | GTGAATTGTGATGGAGTTGT+TGG | + | chr8.2:22201168-22201187 | None:intergenic | 40.0% |
| ! | GTTTCGATCTTCAAGAGCAT+TGG | - | chr8.2:22201407-22201426 | MS.gene011536:CDS | 40.0% |
| ! | TTGTTGTCGTCGTTGTTGTT+TGG | + | chr8.2:22201096-22201115 | None:intergenic | 40.0% |
| !! | AAGCTTTGTTCAAGGACGTT+TGG | - | chr8.2:22200769-22200788 | MS.gene011536:CDS | 40.0% |
| !! | CAGTGGAAGTTGATGAAGAA+GGG | + | chr8.2:22201581-22201600 | None:intergenic | 40.0% |
| !! | GCGAAGAATGGTAATGGTAA+TGG | - | chr8.2:22201462-22201481 | MS.gene011536:CDS | 40.0% |
| GAAACCAAAGCCGAGTTTCA+TGG | - | chr8.2:22201140-22201159 | MS.gene011536:CDS | 45.0% | |
| GTTGTTGGCACCATGAAACT+CGG | + | chr8.2:22201153-22201172 | None:intergenic | 45.0% | |
| TAGATCGTTGTTGACGAGTC+GGG | + | chr8.2:22201059-22201078 | None:intergenic | 45.0% | |
| TCATCTTCTTCAAGTGGAGC+TGG | + | chr8.2:22201666-22201685 | None:intergenic | 45.0% | |
| TGATGCTTTCGCGAGACAAA+TGG | - | chr8.2:22201253-22201272 | MS.gene011536:CDS | 45.0% | |
| TGTTCAAGGACGTTTGGCAA+TGG | - | chr8.2:22200775-22200794 | MS.gene011536:CDS | 45.0% | |
| !! | GCAGTGGAAGTTGATGAAGA+AGG | + | chr8.2:22201582-22201601 | None:intergenic | 45.0% |
| !! | TTTGGCAATGGAAGAGCACT+TGG | - | chr8.2:22200787-22200806 | MS.gene011536:CDS | 45.0% |
| !! | TTTGGTTCTGGTCAAGCACT+TGG | - | chr8.2:22201528-22201547 | MS.gene011536:CDS | 45.0% |
| TGAAGAAGAAGAGGGATCGG+TGG | - | chr8.2:22201314-22201333 | MS.gene011536:CDS | 50.0% | |
| TGTCGCGATGAAGCGAAGAA+TGG | - | chr8.2:22201450-22201469 | MS.gene011536:CDS | 50.0% | |
| ! | AGCACTTGGTGGACACATGA+AGG | - | chr8.2:22200801-22200820 | MS.gene011536:CDS | 50.0% |
| GGCACCATGAAACTCGGCTT+TGG | + | chr8.2:22201147-22201166 | None:intergenic | 55.0% | |
| ! | GGTTCTGGTCAAGCACTTGG+TGG | - | chr8.2:22201531-22201550 | MS.gene011536:CDS | 55.0% |
| !! | GGCAATGGAAGAGCACTTGG+TGG | - | chr8.2:22200790-22200809 | MS.gene011536:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 22200751 | 22201713 | 22200751 | ID=MS.gene011536 |
| chr8.2 | mRNA | 22200751 | 22201713 | 22200751 | ID=MS.gene011536.t1;Parent=MS.gene011536 |
| chr8.2 | exon | 22200751 | 22201713 | 22200751 | ID=MS.gene011536.t1.exon1;Parent=MS.gene011536.t1 |
| chr8.2 | CDS | 22200751 | 22201713 | 22200751 | ID=cds.MS.gene011536.t1;Parent=MS.gene011536.t1 |
| Gene Sequence |
| Protein sequence |
