AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011666

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011666.t1 MTR_0246s0020 75.269 372 45 2 1 325 9 380 1.15e-169 476
MS.gene011666.t1 MTR_0246s0020 71.625 363 54 3 1 316 9 369 4.05e-151 429
MS.gene011666.t1 MTR_6g047570 55.429 350 108 8 1 324 9 336 1.57e-112 330
MS.gene011666.t1 MTR_0011s0260 52.368 359 110 8 10 323 17 359 3.36e-103 307
MS.gene011666.t1 MTR_0011s0210 52.078 361 112 8 10 325 17 361 3.92e-102 304
MS.gene011666.t1 MTR_4g067010 52.326 344 109 9 1 322 9 319 2.78e-99 296
MS.gene011666.t1 MTR_0250s0040 52.848 316 91 8 16 324 1 265 9.88e-92 274
MS.gene011666.t1 MTR_4g066460 52.023 346 119 9 1 324 9 329 2.40e-89 270
MS.gene011666.t1 MTR_4g066380 49.280 347 115 8 8 326 15 328 3.23e-86 263
MS.gene011666.t1 MTR_4g098035 44.054 370 125 12 1 324 9 342 3.06e-78 243
MS.gene011666.t1 MTR_0246s0050 43.380 355 121 10 15 324 2 321 3.34e-75 234
MS.gene011666.t1 MTR_5g014520 52.521 238 95 5 99 323 101 333 6.79e-65 208
MS.gene011666.t1 MTR_5g014560 52.607 211 83 6 122 323 105 307 5.54e-60 194
MS.gene011666.t1 MTR_6g047550 48.594 249 72 6 1 210 9 240 1.16e-58 190
MS.gene011666.t1 MTR_4g009540 50.829 181 85 3 143 320 152 331 1.53e-53 179
MS.gene011666.t1 MTR_2g010450 49.462 186 83 4 141 322 175 353 4.36e-53 178
MS.gene011666.t1 MTR_5g014600 47.549 204 97 5 125 319 132 334 1.83e-50 171
MS.gene011666.t1 MTR_2g104490 41.767 249 129 8 69 314 67 302 2.65e-47 162
MS.gene011666.t1 MTR_4g009110 39.837 246 140 3 76 320 112 350 7.03e-46 159
MS.gene011666.t1 MTR_4g009540 51.250 160 74 3 143 299 152 310 2.20e-45 157
MS.gene011666.t1 MTR_2g104550 39.768 259 141 8 69 323 68 315 3.23e-44 154
MS.gene011666.t1 MTR_4g097940 39.924 263 130 9 71 314 67 320 1.22e-43 152
MS.gene011666.t1 MTR_4g097920 38.783 263 133 9 71 314 52 305 1.40e-43 152
MS.gene011666.t1 MTR_4g097920 38.783 263 133 9 71 314 67 320 2.81e-43 152
MS.gene011666.t1 MTR_4g097950 48.663 187 83 4 143 321 70 251 6.69e-43 149
MS.gene011666.t1 MTR_4g092700 44.809 183 91 4 143 322 158 333 1.10e-42 150
MS.gene011666.t1 MTR_8g009020 43.781 201 95 5 125 315 126 318 1.14e-42 150
MS.gene011666.t1 MTR_2g104490 40.598 234 123 8 69 299 67 287 1.60e-39 141
MS.gene011666.t1 MTR_2g104560 41.304 184 90 5 144 321 55 226 2.96e-38 136
MS.gene011666.t1 MTR_2g104650 42.553 188 104 2 133 318 82 267 7.73e-38 136
MS.gene011666.t1 MTR_7g080780 37.004 227 121 5 102 327 114 319 1.07e-36 134
MS.gene011666.t1 MTR_7g080780 37.004 227 121 5 102 327 127 332 1.36e-36 134
MS.gene011666.t1 MTR_8g069740 37.949 195 108 4 115 298 73 265 3.42e-36 131
MS.gene011666.t1 MTR_5g014640 41.530 183 96 4 139 319 150 323 8.53e-36 132
MS.gene011666.t1 MTR_2g104500 33.600 250 122 6 90 320 48 272 9.33e-35 128
MS.gene011666.t1 MTR_8g009010 31.930 285 131 6 45 315 32 267 1.53e-31 120
MS.gene011666.t1 MTR_2g104530 38.150 173 97 3 144 315 106 269 1.60e-30 117
MS.gene011666.t1 MTR_2g104590 34.197 193 93 6 133 318 57 222 2.51e-29 112
MS.gene011666.t1 MTR_4g009510 40.741 162 63 5 166 317 64 202 5.29e-27 105
MS.gene011666.t1 MTR_7g451650 38.509 161 67 4 166 317 57 194 2.82e-25 100
MS.gene011666.t1 MTR_5g014550 47.287 129 58 3 173 298 54 175 4.06e-24 97.8
MS.gene011666.t1 MTR_7g050350 37.419 155 65 4 172 317 1 132 1.27e-23 94.7
MS.gene011666.t1 MTR_7g451650 37.419 155 65 4 172 317 1 132 1.27e-23 94.7
MS.gene011666.t1 MTR_4g009420 36.250 160 64 3 166 317 65 194 2.39e-23 95.9
MS.gene011666.t1 MTR_1g072320 31.492 181 113 4 143 314 470 648 4.77e-18 85.5
MS.gene011666.t1 MTR_8g067280 35.404 161 94 3 132 284 451 609 1.51e-17 84.0
MS.gene011666.t1 MTR_7g451650 35.664 143 60 4 166 299 57 176 2.90e-16 76.3
MS.gene011666.t1 MTR_8g024790 29.730 148 88 4 143 284 291 428 3.83e-16 79.3
MS.gene011666.t1 MTR_5g030430 30.220 182 109 3 134 303 484 659 1.80e-14 74.7
MS.gene011666.t1 MTR_4g009420 33.099 142 57 3 166 299 65 176 3.62e-14 70.9
MS.gene011666.t1 MTR_8g027495 48.438 64 33 0 143 206 458 521 5.94e-14 72.8
MS.gene011666.t1 MTR_7g117670 53.571 56 26 0 143 198 402 457 9.12e-14 72.4
MS.gene011666.t1 MTR_7g096350 47.692 65 34 0 132 196 344 408 1.06e-13 72.0
MS.gene011666.t1 MTR_8g064700 26.415 159 104 5 145 296 246 398 1.06e-12 68.9
MS.gene011666.t1 MTR_1g112680 25.434 173 106 3 135 284 309 481 1.26e-12 68.9
MS.gene011666.t1 MTR_8g024770 32.773 119 71 4 143 257 279 392 2.96e-12 67.4
MS.gene011666.t1 MTR_8g098275 36.957 92 53 2 135 223 390 479 1.34e-11 65.9
MS.gene011666.t1 MTR_8g098275 36.957 92 53 2 135 223 297 386 1.49e-11 65.5
MS.gene011666.t1 MTR_8g098275 36.957 92 53 2 135 223 233 322 1.55e-11 65.5
MS.gene011666.t1 MTR_3g498695 26.012 173 122 2 144 310 299 471 2.53e-11 64.7
MS.gene011666.t1 MTR_3g030700 28.736 174 118 5 129 298 181 352 8.58e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011666.t1 AT4G37850 35.103 339 182 12 7 322 5 328 2.47e-48 165
MS.gene011666.t1 AT4G37850 35.103 339 182 12 7 322 5 328 2.47e-48 165
MS.gene011666.t1 AT2G22750 45.405 185 101 0 137 321 120 304 3.80e-42 148
MS.gene011666.t1 AT2G22750 45.405 185 101 0 137 321 144 328 6.50e-42 148
MS.gene011666.t1 AT2G22750 45.405 185 101 0 137 321 148 332 7.43e-42 148
MS.gene011666.t1 AT2G22750 45.405 185 100 1 137 321 120 303 9.10e-41 144
MS.gene011666.t1 AT2G22750 45.405 185 100 1 137 321 148 331 1.76e-40 144
MS.gene011666.t1 AT2G22760 42.553 188 98 6 136 317 110 293 9.94e-40 142
MS.gene011666.t1 AT2G22760 42.604 169 88 6 136 299 110 274 4.43e-33 124
MS.gene011666.t1 AT2G22770 35.323 201 89 4 143 317 132 317 6.40e-33 124
MS.gene011666.t1 AT4G17880 29.717 212 128 4 69 277 352 545 1.32e-16 80.9
MS.gene011666.t1 AT1G01260 35.433 127 55 3 132 258 422 521 1.07e-15 78.2
MS.gene011666.t1 AT1G01260 35.433 127 55 3 132 258 422 521 1.07e-15 78.2
MS.gene011666.t1 AT1G01260 35.433 127 55 3 132 258 422 521 1.07e-15 78.2
MS.gene011666.t1 AT4G16430 48.611 72 37 0 132 203 309 380 1.75e-15 77.4
MS.gene011666.t1 AT5G46760 27.751 209 127 7 115 303 388 592 2.36e-15 77.0
MS.gene011666.t1 AT1G32640 32.450 151 88 3 132 277 441 582 2.36e-15 77.4
MS.gene011666.t1 AT2G46510 45.070 71 39 0 132 202 384 454 7.85e-15 75.5
MS.gene011666.t1 AT1G63650 28.025 157 82 4 143 277 405 552 1.95e-13 71.2
MS.gene011666.t1 AT1G63650 28.025 157 82 4 143 277 405 552 1.95e-13 71.2
MS.gene011666.t1 AT1G63650 28.025 157 82 4 143 277 405 552 1.95e-13 71.2
MS.gene011666.t1 AT4G09820 29.814 161 74 4 133 284 351 481 4.03e-13 70.5
MS.gene011666.t1 AT2G16910 28.313 166 92 4 144 287 315 475 1.76e-12 68.6
MS.gene011666.t1 AT5G41315 25.926 162 96 3 137 277 440 598 3.06e-12 67.8
MS.gene011666.t1 AT5G41315 25.926 162 96 3 137 277 443 601 3.15e-12 67.8
MS.gene011666.t1 AT5G41315 25.926 162 96 3 137 277 435 593 3.25e-12 67.8
MS.gene011666.t1 AT4G00870 32.800 125 64 2 144 259 250 363 5.24e-12 66.6
MS.gene011666.t1 AT5G57150 27.222 180 116 5 131 300 42 216 9.70e-12 63.9
MS.gene011666.t1 AT3G26744 27.273 165 114 2 144 302 308 472 1.21e-11 65.9
MS.gene011666.t1 AT3G26744 27.273 165 114 2 144 302 308 472 1.21e-11 65.9
MS.gene011666.t1 AT3G26744 27.273 165 114 2 144 302 308 472 1.21e-11 65.9
MS.gene011666.t1 AT5G57150 27.374 179 115 5 131 299 41 214 1.26e-11 63.9
MS.gene011666.t1 AT5G57150 27.374 179 115 5 131 299 42 215 1.30e-11 64.3
MS.gene011666.t1 AT5G57150 27.374 179 115 5 131 299 42 215 1.68e-11 63.5
MS.gene011666.t1 AT5G57150 27.624 181 116 5 131 301 41 216 1.69e-11 63.9
MS.gene011666.t1 AT3G26744 28.058 139 95 1 144 277 308 446 3.55e-11 64.3
MS.gene011666.t1 AT5G46830 41.667 72 42 0 134 205 334 405 5.46e-11 63.9

Find 60 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGTAGGAGGGAGAATT+TGG 0.138604 8.2:+24159507 None:intergenic
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8.2:+24158673 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8.2:-24159938 MS.gene011666:intron
TCTGTTTGACTTTGAGTTTA+AGG 0.248045 8.2:+24159457 None:intergenic
GACATGAATGTTTCTCATTT+GGG 0.281293 8.2:+24159409 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8.2:+24159408 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8.2:+24159785 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8.2:+24159799 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8.2:-24158673 MS.gene011666:CDS
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8.2:-24159235 MS.gene011666:intron
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8.2:-24158605 MS.gene011666:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8.2:+24159939 None:intergenic
GTTGGTAGGAGGGAGAATTT+GGG 0.373658 8.2:+24159508 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8.2:+24159572 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8.2:-24158606 MS.gene011666:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8.2:+24159298 None:intergenic
CTGAACTCAAACATTGTTGT+TGG 0.389224 8.2:+24159735 None:intergenic
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8.2:+24159259 None:intergenic
CATCATCATCAATGATACTA+TGG 0.407350 8.2:+24158805 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8.2:-24159389 MS.gene011666:CDS
TGAACTCAAACATTGTTGTT+GGG 0.428381 8.2:+24159736 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8.2:-24158704 MS.gene011666:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8.2:-24158723 MS.gene011666:CDS
CAGGAGTGAGTCCACAAACC+TGG 0.442702 8.2:+24159480 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8.2:-24159388 MS.gene011666:intron
TCATATGATGAGAGTATTGA+AGG 0.466240 8.2:-24158765 MS.gene011666:CDS
AGGAGTGAGTCCACAAACCT+GGG 0.476028 8.2:+24159481 None:intergenic
TTTGACTTTGAGTTTAAGGC+AGG 0.485881 8.2:+24159461 None:intergenic
TGACATTACTATCATTGCTC+AGG 0.494765 8.2:-24158574 MS.gene011666:intron
AGCAATGATAGTAATGTCAA+TGG 0.495292 8.2:+24158578 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8.2:+24159305 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8.2:+24159311 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8.2:-24159782 MS.gene011666:CDS
TCATCAATGATACTATGGTC+TGG 0.549545 8.2:+24158810 None:intergenic
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8.2:+24159776 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8.2:-24159951 MS.gene011666:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8.2:+24159413 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8.2:-24159310 MS.gene011666:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8.2:+24159560 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8.2:-24158604 MS.gene011666:CDS
TAAAATTTGGTAGATGGACA+AGG 0.590351 8.2:-24158946 MS.gene011666:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8.2:+24158591 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8.2:-24157598 MS.gene011666:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8.2:+24157562 None:intergenic
GGAGTGAGTCCACAAACCTG+GGG 0.605429 8.2:+24159482 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8.2:-24158661 MS.gene011666:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8.2:-24158682 MS.gene011666:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8.2:-24158680 MS.gene011666:CDS
GGTAGGAGGGAGAATTTGGG+AGG 0.613050 8.2:+24159511 None:intergenic
AATGTCAATGGAATCCCCAA+AGG 0.614554 8.2:+24158590 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8.2:+24159410 None:intergenic
TGAAATTGAGAGACCAACCA+TGG 0.632157 8.2:-24159642 MS.gene011666:intron
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8.2:-24158722 MS.gene011666:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8.2:-24158847 MS.gene011666:CDS
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8.2:+24158830 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8.2:-24158681 MS.gene011666:CDS
GAGTGAGTCCACAAACCTGG+GGG 0.677152 8.2:+24159483 None:intergenic
AGAACAAAACAAGAAAACAA+AGG 0.679620 8.2:-24158859 MS.gene011666:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8.2:-24157574 MS.gene011666:CDS
AGTGAGTCCACAAACCTGGG+GGG 0.749063 8.2:+24159484 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAGTATAGAAATAATTTAT+TGG - chr8.2:24159482-24159501 MS.gene011666:CDS 15.0%
!! TCAAATACTGTTATAAAATT+TGG - chr8.2:24158538-24158557 MS.gene011666:intron 15.0%
!! TTTAAACAATGAATATTTAG+TGG + chr8.2:24158981-24159000 None:intergenic 15.0%
!!! AACTTGTTAAGAATAAAAAA+AGG - chr8.2:24159107-24159126 MS.gene011666:intron 15.0%
!!! AAGTATTATTATTTTGTTTG+AGG - chr8.2:24159036-24159055 MS.gene011666:intron 15.0%
!!! ATTAAATGCACAAAATTTTA+AGG - chr8.2:24159543-24159562 MS.gene011666:CDS 15.0%
!!! ATTATTACATTTTTTGTCTA+GGG - chr8.2:24159270-24159289 MS.gene011666:CDS 15.0%
!! AAGGAATTTATTACCATAAA+AGG - chr8.2:24159126-24159145 MS.gene011666:intron 20.0%
!! ATAATTAATGAAATGCTAAC+CGG + chr8.2:24159243-24159262 None:intergenic 20.0%
!! ATATTTAGTGGCAATTATTA+AGG + chr8.2:24158969-24158988 None:intergenic 20.0%
!!! AACTTGTGTTGTTTTTTTTT+TGG - chr8.2:24159419-24159438 MS.gene011666:CDS 20.0%
!!! ATTGTTATACTTTTGTAGAT+GGG - chr8.2:24159873-24159892 MS.gene011666:intron 20.0%
!!! ATTTTTTTTTTTGTTGTTGC+AGG - chr8.2:24157652-24157671 MS.gene011666:intron 20.0%
!!! CACTACTTTTTTTTTATATC+AGG - chr8.2:24159618-24159637 MS.gene011666:CDS 20.0%
!!! CATTATTACATTTTTTGTCT+AGG - chr8.2:24159269-24159288 MS.gene011666:CDS 20.0%
!!! GATTTGGTAAGAAAATTAAA+GGG - chr8.2:24157575-24157594 MS.gene011666:CDS 20.0%
!!! TATTGTTATACTTTTGTAGA+TGG - chr8.2:24159872-24159891 MS.gene011666:intron 20.0%
!!! TCTTTTTAGGAATTTTGTTT+AGG - chr8.2:24159781-24159800 MS.gene011666:CDS 20.0%
!!! TGATTTGGTAAGAAAATTAA+AGG - chr8.2:24157574-24157593 MS.gene011666:CDS 20.0%
!!! TTATTACATTTTTTGTCTAG+GGG - chr8.2:24159271-24159290 MS.gene011666:CDS 20.0%
!!! TTTTCTATCTGTTTCTTTTT+AGG - chr8.2:24159768-24159787 MS.gene011666:CDS 20.0%
! AATACAATTAAACAACTCGT+GGG + chr8.2:24159845-24159864 None:intergenic 25.0%
! AGAACAAAACAAGAAAACAA+AGG - chr8.2:24158638-24158657 MS.gene011666:CDS 25.0%
! AGTAGTGAAAAAATGTTTAG+TGG + chr8.2:24159012-24159031 None:intergenic 25.0%
! CTGTTATAAAATTTGGTAGA+TGG - chr8.2:24158545-24158564 MS.gene011666:intron 25.0%
! TAATACAATTAAACAACTCG+TGG + chr8.2:24159846-24159865 None:intergenic 25.0%
! TAATTAATGAAATGCTAACC+GGG + chr8.2:24159242-24159261 None:intergenic 25.0%
! TATTACACGTATTCATGAAA+GGG - chr8.2:24159722-24159741 MS.gene011666:intron 25.0%
! TTATTACACGTATTCATGAA+AGG - chr8.2:24159721-24159740 MS.gene011666:intron 25.0%
!! TAAGGAATACTTCTTCTTTT+GGG - chr8.2:24159561-24159580 MS.gene011666:CDS 25.0%
!! TGTTGGCTGTTATAATTAAT+TGG + chr8.2:24159677-24159696 None:intergenic 25.0%
!! TTAAGGAATACTTCTTCTTT+TGG - chr8.2:24159560-24159579 MS.gene011666:CDS 25.0%
!!! AAAGTATCGTCAATAATTGT+TGG + chr8.2:24159694-24159713 None:intergenic 25.0%
!!! AATAATTAACTTGTGCCTTA+AGG + chr8.2:24159462-24159481 None:intergenic 25.0%
!!! ATAATTAACTTGTGCCTTAA+GGG + chr8.2:24159461-24159480 None:intergenic 25.0%
AAAGATGAGTTAGATGATGA+TGG + chr8.2:24157940-24157959 None:intergenic 30.0%
AATATCATTAATCAATGCCC+CGG - chr8.2:24159221-24159240 MS.gene011666:intron 30.0%
AATTAATGAAATGCTAACCG+GGG + chr8.2:24159241-24159260 None:intergenic 30.0%
ACAAAAACATCTCAAACCAA+AGG - chr8.2:24158156-24158175 MS.gene011666:intron 30.0%
ACATGAATGTTTCTCATTTG+GGG + chr8.2:24158090-24158109 None:intergenic 30.0%
AGACATGAATGTTTCTCATT+TGG + chr8.2:24158092-24158111 None:intergenic 30.0%
AGCAATGATAGTAATGTCAA+TGG + chr8.2:24158922-24158941 None:intergenic 30.0%
ATTATTAGGGTTATGCTAAC+CGG - chr8.2:24159074-24159093 MS.gene011666:intron 30.0%
CATCATCATCAATGATACTA+TGG + chr8.2:24158695-24158714 None:intergenic 30.0%
GACATGAATGTTTCTCATTT+GGG + chr8.2:24158091-24158110 None:intergenic 30.0%
TAAAATTTGGTAGATGGACA+AGG - chr8.2:24158551-24158570 MS.gene011666:intron 30.0%
TCTCAACTCAAAATCCTAAA+GGG - chr8.2:24158109-24158128 MS.gene011666:intron 30.0%
TTAGTGGCAATTATTAAGGT+AGG + chr8.2:24158965-24158984 None:intergenic 30.0%
! AAGGAATACTTCTTCTTTTG+GGG - chr8.2:24159562-24159581 MS.gene011666:CDS 30.0%
! GTTAACGAAGTTTCCTTTTA+TGG + chr8.2:24159142-24159161 None:intergenic 30.0%
! TCTGTTTGACTTTGAGTTTA+AGG + chr8.2:24158043-24158062 None:intergenic 30.0%
! TGAACTCAAACATTGTTGTT+GGG + chr8.2:24157764-24157783 None:intergenic 30.0%
!! TCATATGATGAGAGTATTGA+AGG - chr8.2:24158732-24158751 MS.gene011666:CDS 30.0%
!!! AACATTGATTTTGAAAGCGA+GGG + chr8.2:24159318-24159337 None:intergenic 30.0%
!!! ACTTTTGACTCTTTTGACTT+TGG - chr8.2:24157830-24157849 MS.gene011666:intron 30.0%
!!! TAAAAAGCGTTTGGAAGTTT+TGG - chr8.2:24158614-24158633 MS.gene011666:CDS 30.0%
!!! TGTTGCAAAGTTTTGAGTTT+TGG + chr8.2:24158139-24158158 None:intergenic 30.0%
!!! TTTTTTAACATGTGCCCTAA+GGG - chr8.2:24159585-24159604 MS.gene011666:CDS 30.0%
!!! TTTTTTGTTGTTGCAGGAAA+TGG - chr8.2:24157658-24157677 MS.gene011666:intron 30.0%
AAACTTCCTTGATGCTAATG+AGG - chr8.2:24157715-24157734 MS.gene011666:intron 35.0%
AATGTGTATTAGTGTCGTGT+TGG + chr8.2:24158343-24158362 None:intergenic 35.0%
CACTTCACACTTATGTTTGA+AGG - chr8.2:24158288-24158307 MS.gene011666:intron 35.0%
CATTAGCATCAAGGAAGTTT+AGG + chr8.2:24157715-24157734 None:intergenic 35.0%
CTGAACTCAAACATTGTTGT+TGG + chr8.2:24157765-24157784 None:intergenic 35.0%
GTCTCAACTCAAAATCCTAA+AGG - chr8.2:24158108-24158127 MS.gene011666:intron 35.0%
TAACAAGTTTCTCAGAGTAC+CGG + chr8.2:24159096-24159115 None:intergenic 35.0%
TACTATCATTGCTCAGGTAA+AGG - chr8.2:24158929-24158948 MS.gene011666:CDS 35.0%
TATCAGAAGAAACCAGCAAA+TGG - chr8.2:24157546-24157565 MS.gene011666:CDS 35.0%
TCATCAATGATACTATGGTC+TGG + chr8.2:24158690-24158709 None:intergenic 35.0%
TCTTGTTCCTAACCTGAAAA+AGG - chr8.2:24158262-24158281 MS.gene011666:intron 35.0%
TGACATTACTATCATTGCTC+AGG - chr8.2:24158923-24158942 MS.gene011666:CDS 35.0%
TGTTTCATCAGTGAATGAGT+TGG + chr8.2:24157810-24157829 None:intergenic 35.0%
! ATACTTTTGTAGATGGGTGA+AGG - chr8.2:24159879-24159898 MS.gene011666:intron 35.0%
! TACTTTTGTAGATGGGTGAA+GGG - chr8.2:24159880-24159899 MS.gene011666:intron 35.0%
! TTGGTGATTAGTTGTGGAAT+TGG + chr8.2:24157791-24157810 None:intergenic 35.0%
! TTTGACTTTGAGTTTAAGGC+AGG + chr8.2:24158039-24158058 None:intergenic 35.0%
!! AACATTTGTTTTCAGCTCCA+TGG + chr8.2:24157875-24157894 None:intergenic 35.0%
!! AAGTTTAGGTTGCATTCTTC+AGG + chr8.2:24157701-24157720 None:intergenic 35.0%
!! AATGAGTTGGTGATTAGTTG+TGG + chr8.2:24157797-24157816 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG - chr8.2:24159444-24159463 MS.gene011666:CDS 35.0%
!! GAAAGAGCTTAAAAAGCGTT+TGG - chr8.2:24158605-24158624 MS.gene011666:CDS 35.0%
!! TGGAGAAAGTTTTGGTGAAA+TGG + chr8.2:24157920-24157939 None:intergenic 35.0%
!! TGTGAAGTGTTACCTTTTTC+AGG + chr8.2:24158277-24158296 None:intergenic 35.0%
!! TTGAGTGCTATTTAGCTAGT+GGG + chr8.2:24158473-24158492 None:intergenic 35.0%
!! TTTGAGTGCTATTTAGCTAG+TGG + chr8.2:24158474-24158493 None:intergenic 35.0%
!!! AGTGTTACCTTTTTCAGGTT+AGG + chr8.2:24158272-24158291 None:intergenic 35.0%
!!! CAACATTGATTTTGAAAGCG+AGG + chr8.2:24159319-24159338 None:intergenic 35.0%
!!! GATGATGATGGAGAAAGTTT+TGG + chr8.2:24157928-24157947 None:intergenic 35.0%
!!! GTTTTTTAACATGTGCCCTA+AGG - chr8.2:24159584-24159603 MS.gene011666:CDS 35.0%
!!! TGAGTTTTGGATAACCCTTT+AGG + chr8.2:24158126-24158145 None:intergenic 35.0%
AATAGCAGTGTCTTACCCTT+TGG - chr8.2:24158891-24158910 MS.gene011666:CDS 40.0%
AATGTCAATGGAATCCCCAA+AGG + chr8.2:24158910-24158929 None:intergenic 40.0%
ACACGTATTCATGAAAGGGA+GGG - chr8.2:24159726-24159745 MS.gene011666:intron 40.0%
ACTCTTCCTCATTAGCATCA+AGG + chr8.2:24157724-24157743 None:intergenic 40.0%
AGGGTACAACTTGAGCATAA+AGG - chr8.2:24159899-24159918 MS.gene011666:intron 40.0%
ATAGCAGTGTCTTACCCTTT+GGG - chr8.2:24158892-24158911 MS.gene011666:CDS 40.0%
ATGTCAATGGAATCCCCAAA+GGG + chr8.2:24158909-24158928 None:intergenic 40.0%
CAAATCAGAAAGCCATTTGC+TGG + chr8.2:24157561-24157580 None:intergenic 40.0%
CAGCAAATGGCTTTCTGATT+TGG - chr8.2:24157559-24157578 MS.gene011666:CDS 40.0%
GCTTTCTTTCAGCCATGATA+TGG + chr8.2:24158202-24158221 None:intergenic 40.0%
TACACGTATTCATGAAAGGG+AGG - chr8.2:24159725-24159744 MS.gene011666:intron 40.0%
TGAAATTGAGAGACCAACCA+TGG - chr8.2:24157855-24157874 MS.gene011666:intron 40.0%
TGAATGTTTCTCATTTGGGG+TGG + chr8.2:24158087-24158106 None:intergenic 40.0%
TGGTTTCTTCAGAACAACCA+CGG + chr8.2:24158670-24158689 None:intergenic 40.0%
TTACTGTCGATGTTGTGTCT+TGG - chr8.2:24158412-24158431 MS.gene011666:intron 40.0%
TTCAGCCATGATATGGTCAT+CGG + chr8.2:24158195-24158214 None:intergenic 40.0%
! CACTTTTGCTTGCACTTGAA+AGG + chr8.2:24158772-24158791 None:intergenic 40.0%
!! AAGTGCAAGCAAAAGTGTCA+GGG - chr8.2:24158775-24158794 MS.gene011666:CDS 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG + chr8.2:24159602-24159621 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG + chr8.2:24159603-24159622 None:intergenic 40.0%
!! TGAGTGCTATTTAGCTAGTG+GGG + chr8.2:24158472-24158491 None:intergenic 40.0%
!! TTTGTTTTCAGCTCCATGGT+TGG + chr8.2:24157871-24157890 None:intergenic 40.0%
ACTTGTCAAGAACCTACGCA+TGG - chr8.2:24159923-24159942 MS.gene011666:intron 45.0%
CTGCAAGAGCAATGAAGCTT+TGG + chr8.2:24158241-24158260 None:intergenic 45.0%
GAAAACAAAGGTAGAGTCCG+TGG - chr8.2:24158650-24158669 MS.gene011666:CDS 45.0%
TAGCAGTGTCTTACCCTTTG+GGG - chr8.2:24158893-24158912 MS.gene011666:CDS 45.0%
! ATTCACTGCCAGAAGCACAA+GGG - chr8.2:24158816-24158835 MS.gene011666:CDS 45.0%
! CAGGGAAAGAGATGCTGATT+CGG - chr8.2:24158793-24158812 MS.gene011666:CDS 45.0%
! GTTGGTAGGAGGGAGAATTT+GGG + chr8.2:24157992-24158011 None:intergenic 45.0%
! TGTTGGTAGGAGGGAGAATT+TGG + chr8.2:24157993-24158012 None:intergenic 45.0%
!! CAAGTGCAAGCAAAAGTGTC+AGG - chr8.2:24158774-24158793 MS.gene011666:CDS 45.0%
!! GGGAGGGCGTATTTTATTCT+AGG - chr8.2:24159742-24159761 MS.gene011666:intron 45.0%
!! ACTAATTATTATTAATTATT+AGG - chr8.2:24159060-24159079 MS.gene011666:intron 5.0%
!! CTAATTATTATTAATTATTA+GGG - chr8.2:24159061-24159080 MS.gene011666:intron 5.0%
ACTTCAATGCTGCCATGCGT+AGG + chr8.2:24159938-24159957 None:intergenic 50.0%
AGGAGTGAGTCCACAAACCT+GGG + chr8.2:24158019-24158038 None:intergenic 50.0%
CAACGCCGATGACCATATCA+TGG - chr8.2:24158187-24158206 MS.gene011666:intron 50.0%
CATGATATGGTCATCGGCGT+TGG + chr8.2:24158189-24158208 None:intergenic 50.0%
GGGGATTCTGGTGAAAGTCA+TGG - chr8.2:24158836-24158855 MS.gene011666:CDS 50.0%
GGTCATCTGAGTGACACGTT+TGG + chr8.2:24159297-24159316 None:intergenic 50.0%
! GATTCACTGCCAGAAGCACA+AGG - chr8.2:24158815-24158834 MS.gene011666:CDS 50.0%
! TTCACTGCCAGAAGCACAAG+GGG - chr8.2:24158817-24158836 MS.gene011666:CDS 50.0%
AGTGAGTCCACAAACCTGGG+GGG + chr8.2:24158016-24158035 None:intergenic 55.0%
CAGGAGTGAGTCCACAAACC+TGG + chr8.2:24158020-24158039 None:intergenic 55.0%
CCAGAATCCCCTTGTGCTTC+TGG + chr8.2:24158827-24158846 None:intergenic 55.0%
GAGTGAGTCCACAAACCTGG+GGG + chr8.2:24158017-24158036 None:intergenic 55.0%
GGAGTGAGTCCACAAACCTG+GGG + chr8.2:24158018-24158037 None:intergenic 55.0%
GGTAGGAGGGAGAATTTGGG+AGG + chr8.2:24157989-24158008 None:intergenic 55.0%
! CCAGAAGCACAAGGGGATTC+TGG - chr8.2:24158824-24158843 MS.gene011666:CDS 55.0%
ACCAACACCCCCCAGGTTTG+TGG - chr8.2:24158006-24158025 MS.gene011666:intron 60.0%
! TCCACAAACCTGGGGGGTGT+TGG + chr8.2:24158010-24158029 None:intergenic 60.0%
!! CAAACCTGGGGGGTGTTGGT+AGG + chr8.2:24158006-24158025 None:intergenic 60.0%
!! ACCTGGGGGGTGTTGGTAGG+AGG + chr8.2:24158003-24158022 None:intergenic 65.0%
!! CCTGGGGGGTGTTGGTAGGA+GGG + chr8.2:24158002-24158021 None:intergenic 65.0%
!! CGGCGTTGGCTCGAGACCTT+TGG + chr8.2:24158175-24158194 None:intergenic 65.0%
CCCTCCTACCAACACCCCCC+AGG - chr8.2:24157999-24158018 MS.gene011666:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 24157539 24159980 24157539 ID=MS.gene011666
chr8.2 mRNA 24157539 24159980 24157539 ID=MS.gene011666.t1;Parent=MS.gene011666
chr8.2 exon 24159939 24159980 24159939 ID=MS.gene011666.t1.exon1;Parent=MS.gene011666.t1
chr8.2 CDS 24159939 24159980 24159939 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
chr8.2 exon 24159747 24159845 24159747 ID=MS.gene011666.t1.exon2;Parent=MS.gene011666.t1
chr8.2 CDS 24159747 24159845 24159747 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
chr8.2 exon 24159389 24159654 24159389 ID=MS.gene011666.t1.exon3;Parent=MS.gene011666.t1
chr8.2 CDS 24159389 24159654 24159389 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
chr8.2 exon 24159236 24159341 24159236 ID=MS.gene011666.t1.exon4;Parent=MS.gene011666.t1
chr8.2 CDS 24159236 24159341 24159236 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
chr8.2 exon 24158575 24158955 24158575 ID=MS.gene011666.t1.exon5;Parent=MS.gene011666.t1
chr8.2 CDS 24158575 24158955 24158575 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
chr8.2 exon 24157539 24157628 24157539 ID=MS.gene011666.t1.exon6;Parent=MS.gene011666.t1
chr8.2 CDS 24157539 24157628 24157539 ID=cds.MS.gene011666.t1;Parent=MS.gene011666.t1
Gene Sequence

>MS.gene011666

ATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGTAAGAAAATTAAAGGGCAAATTAACATATTATTGATTATATAAGTAAATTCTTTATCATTTTAAATTTTAATTTTTTTTTTTGTTGTTGCAGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTAATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAAATGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTGTCTTTTGACAACCTCCCAAATTCTCCCTCCTACCAACACCCCCCAGGTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAACAGAACAAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCATACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTTATGCTAACCGGTACTCTGAGAAACTTGTTAAGAATAAAAAAAGGAATTTATTACCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTGTCTAGGGGCCAAACGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTGTTTTTTTTTTGGAAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTGTAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAACTTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATACTTCTTCTTTTGGGGTTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTTATATCAGGATGTTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>MS.gene011666.t1

MNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLHKNKPNNNRPTMELKTNVSDNNIIETISPKLSPSSSNSSLHSQICLLTTSQILPPTNTPQVCGLTPALNSKSNRTKTVSVFPPQMRNIHVSTQNPKGSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSIQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE