AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011713

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011713.t1 MTR_4g097130 97.411 309 6 2 1 307 1 309 0.0 597
MS.gene011713.t1 MTR_4g097130 97.403 308 6 2 1 306 1 308 0.0 594
MS.gene011713.t1 MTR_4g097130 95.847 313 6 3 1 306 1 313 0.0 588
MS.gene011713.t1 MTR_5g015340 64.935 308 91 5 1 306 1 293 9.51e-104 305
MS.gene011713.t1 MTR_5g015340 64.935 308 91 5 1 306 1 293 9.51e-104 305
MS.gene011713.t1 MTR_5g015340 65.049 309 91 5 1 307 1 294 1.14e-103 308
MS.gene011713.t1 MTR_2g031870 45.455 253 120 5 1 249 1 239 2.91e-67 212
MS.gene011713.t1 MTR_8g494240 44.706 255 117 5 1 249 1 237 1.15e-64 206
MS.gene011713.t1 MTR_8g494240 44.706 255 117 5 1 249 1 237 1.16e-64 206
MS.gene011713.t1 MTR_8g494240 44.706 255 117 5 1 249 1 237 2.97e-63 205
MS.gene011713.t1 MTR_8g092810 40.602 266 131 6 1 253 1 252 4.75e-54 182
MS.gene011713.t1 MTR_2g031870 37.945 253 113 6 1 249 1 213 7.01e-46 156
MS.gene011713.t1 MTR_0095s0040 49.704 169 65 5 1 163 1 155 1.83e-42 147
MS.gene011713.t1 MTR_1g041765 69.048 84 25 1 1 84 1 83 8.68e-33 120
MS.gene011713.t1 MTR_8g018410 35.484 279 155 7 1 279 1 254 2.18e-32 121
MS.gene011713.t1 MTR_2g099610 37.500 216 114 5 1 201 1 210 2.90e-32 121
MS.gene011713.t1 MTR_1g088180 37.705 122 67 5 136 254 296 411 2.84e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011713.t1 AT5G67580 50.160 313 136 6 1 307 1 299 1.39e-70 221
MS.gene011713.t1 AT5G67580 50.160 313 136 6 1 307 1 299 1.39e-70 221
MS.gene011713.t1 AT3G49850 46.885 305 145 5 1 303 1 290 4.08e-64 204
MS.gene011713.t1 AT3G49850 46.885 305 145 5 1 303 1 290 4.08e-64 204
MS.gene011713.t1 AT1G49950 43.191 257 123 7 1 251 1 240 3.19e-61 197
MS.gene011713.t1 AT1G49950 43.191 257 123 7 1 251 1 240 3.19e-61 197
MS.gene011713.t1 AT1G49950 43.191 257 123 7 1 251 1 240 3.19e-61 197
MS.gene011713.t1 AT1G72740 33.101 287 146 8 1 279 1 249 2.48e-30 116
MS.gene011713.t1 AT1G72740 32.881 295 142 10 1 279 1 255 3.53e-28 110
MS.gene011713.t1 AT1G17520 36.408 206 120 4 1 203 1 198 2.95e-27 108
MS.gene011713.t1 AT1G17520 36.715 207 119 5 1 203 1 199 6.77e-26 104
MS.gene011713.t1 AT1G17520 36.735 196 113 4 1 193 1 188 1.12e-25 103
MS.gene011713.t1 AT1G17520 37.056 197 112 5 1 193 1 189 2.26e-24 100

Find 75 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAGCCCCTGACAATTTC+TGG 0.156688 8.3:-22167657 MS.gene011713:CDS
AATGCAAAATGCTTCATATT+TGG 0.195627 8.3:-22165388 MS.gene011713:CDS
TTGATAGCTCTGCCTCAATT+TGG 0.196523 8.3:+22165579 None:intergenic
CAATTGGTTATTGTCAGTTT+TGG 0.201241 8.3:+22167719 None:intergenic
ATTCGAACGCGTACGCAAAA+TGG 0.224697 8.3:+22167958 None:intergenic
GACAATAACTTTCTAAGATT+TGG 0.247003 8.3:+22166504 None:intergenic
CTTCACCTCCGAAGAATCTT+TGG 0.250581 8.3:+22165631 None:intergenic
TGTATTGTGAACAGGATAAA+TGG 0.295045 8.3:-22167815 MS.gene011713:intron
TGATAGCTCTGCCTCAATTT+GGG 0.303115 8.3:+22165580 None:intergenic
ATGTTCCACTACAAATAGTT+AGG 0.338025 8.3:+22167610 None:intergenic
TCAAACACGGGGCTGGAAAA+TGG 0.376865 8.3:-22168012 MS.gene011713:CDS
TCTGCAGTCCATTTCTGCTT+AGG 0.379164 8.3:+22168064 None:intergenic
CTACTTTACTCAAGGCCAAT+TGG 0.386042 8.3:+22167703 None:intergenic
ATATAAATGTCACAGCAATA+TGG 0.388272 8.3:-22167788 MS.gene011713:CDS
CGCCTCCCTAGCTGCTGCTT+CGG 0.390489 8.3:+22165475 None:intergenic
CTACAAATAGTTAGGGGCTT+AGG 0.415247 8.3:+22167618 None:intergenic
ATGTTCCACCAGAAATTGTC+AGG 0.418565 8.3:+22167649 None:intergenic
TTCTCAGAGACCGCTGAACT+TGG 0.424839 8.3:+22165692 None:intergenic
GAAGAAGCGGCGTTGAAAGC+TGG 0.427269 8.3:-22168040 MS.gene011713:CDS
TGGGTGCTCCTAAGCAGAAA+TGG 0.427716 8.3:-22168072 MS.gene011713:CDS
TGCTGCTTCGGCCTGTGCAA+TGG 0.434469 8.3:+22165487 None:intergenic
TATAAATGTCACAGCAATAT+GGG 0.435123 8.3:-22167787 MS.gene011713:CDS
GGGAGACAATAATGTATTAG+AGG 0.441902 8.3:-22166909 MS.gene011713:CDS
AATAACTTTCTAAGATTTGG+TGG 0.446528 8.3:+22166507 None:intergenic
GCAGCCTTGTCAGAACCCTT+TGG 0.463786 8.3:+22166866 None:intergenic
AACGAGGTCCAAAGATTCTT+CGG 0.464352 8.3:-22165639 MS.gene011713:CDS
AGCTGCAAAAGCAGTTGCAG+AGG 0.469233 8.3:-22165519 MS.gene011713:CDS
CGAAGCAGCAGCTAGGGAGG+CGG 0.477225 8.3:-22165474 MS.gene011713:CDS
CAAAACTGACAATAACCAAT+TGG 0.479938 8.3:-22167718 MS.gene011713:CDS
GGAGTAGTCAAACACGGGGC+TGG 0.481269 8.3:-22168019 MS.gene011713:CDS
CTTTGCAAAAGCAGCGATGA+AGG 0.481859 8.3:-22165417 MS.gene011713:CDS
ACAGGCCGAAGCAGCAGCTA+GGG 0.483254 8.3:-22165480 MS.gene011713:CDS
GGCCGAAGCAGCAGCTAGGG+AGG 0.499766 8.3:-22165477 MS.gene011713:CDS
TGTTCCACTACAAATAGTTA+GGG 0.500173 8.3:+22167611 None:intergenic
CACAGGCCGAAGCAGCAGCT+AGG 0.511915 8.3:-22165481 MS.gene011713:CDS
TGCCATTGCTTCTTACATAG+AGG 0.516855 8.3:-22166846 MS.gene011713:intron
GTCAATATGAAGGAGCCAAA+GGG 0.526790 8.3:-22166881 MS.gene011713:CDS
GTTCAAAAGAACAAGTATCA+AGG 0.540828 8.3:-22167575 MS.gene011713:intron
CCTTTCAAAATCCCAAATTG+AGG 0.542802 8.3:-22165591 MS.gene011713:CDS
TACCTCTATGTAAGAAGCAA+TGG 0.545469 8.3:+22166844 None:intergenic
TCACAGCAATATGGGGATCC+CGG 0.546612 8.3:-22167779 MS.gene011713:CDS
GGCAGAAGTTGCCATTGCAC+AGG 0.551573 8.3:-22165498 MS.gene011713:CDS
CGCAAAATGGAACTGAACTC+AGG 0.555443 8.3:+22167971 None:intergenic
TTTCTGCTTAGGAGCACCCA+TGG 0.559262 8.3:+22168075 None:intergenic
AGAGGCTATTGTCAATATGA+AGG 0.562785 8.3:-22166891 MS.gene011713:CDS
CTCTGATGCTTGTGGAAACG+AGG 0.563209 8.3:-22165655 MS.gene011713:CDS
AAGCTCTTCTCTGATGCTTG+TGG 0.564200 8.3:-22165663 MS.gene011713:CDS
TGTCAATATGAAGGAGCCAA+AGG 0.567981 8.3:-22166882 MS.gene011713:CDS
GTCAGCAAAATTGCAGCAGA+TGG 0.568574 8.3:-22166484 MS.gene011713:CDS
GACAAGTGGCAAAATAGTCC+AGG 0.570447 8.3:-22166460 MS.gene011713:intron
AAAATGGCGCACAATACTCA+TGG 0.571706 8.3:-22167996 MS.gene011713:CDS
TCAGCGGTCTCTGAGAAAAG+AGG 0.571845 8.3:-22165686 MS.gene011713:CDS
ACGTTGAACTACTTTACTCA+AGG 0.573317 8.3:+22167695 None:intergenic
GGCAGAGCTATCAAAAGTGA+GGG 0.588740 8.3:-22165570 MS.gene011713:CDS
CAAAATTATGCCAAGTTCAG+CGG 0.601681 8.3:-22165702 MS.gene011713:CDS
AAGCCCCTAACTATTTGTAG+TGG 0.611891 8.3:-22167615 MS.gene011713:CDS
GCGTTCGAATGTAGATCTCA+AGG 0.619214 8.3:-22167945 MS.gene011713:intron
AAGCTGGAGTAGTCAAACAC+GGG 0.628346 8.3:-22168024 MS.gene011713:CDS
AGGCAGAGCTATCAAAAGTG+AGG 0.628730 8.3:-22165571 MS.gene011713:CDS
GATTGCAGAAGCTGAAGCTG+AGG 0.636628 8.3:-22165450 MS.gene011713:CDS
GGGGATGTCAGCTCAAGAGG+CGG 0.637055 8.3:-22165549 MS.gene011713:CDS
AAGCCCCTGACAATTTCTGG+TGG 0.637720 8.3:-22167654 MS.gene011713:CDS
GGAGCCAAAGGGTTCTGACA+AGG 0.637821 8.3:-22166870 MS.gene011713:CDS
TTGCAGCAGATGGTGACAAG+TGG 0.648634 8.3:-22166474 MS.gene011713:CDS
ATAAATGTCACAGCAATATG+GGG 0.648999 8.3:-22167786 MS.gene011713:CDS
TGTTCCACCAGAAATTGTCA+GGG 0.649357 8.3:+22167650 None:intergenic
ATGGACTGCAGAAGAAGAAG+CGG 0.652524 8.3:-22168053 MS.gene011713:CDS
ATTGTGAACAGGATAAATGG+AGG 0.664041 8.3:-22167812 MS.gene011713:intron
CAGAGCTATCAAAAGTGAGG+GGG 0.676061 8.3:-22165568 MS.gene011713:CDS
AAAGCTGGAGTAGTCAAACA+CGG 0.681254 8.3:-22168025 MS.gene011713:CDS
GTTCCACTACAAATAGTTAG+GGG 0.682877 8.3:+22167612 None:intergenic
GCAGAGCTATCAAAAGTGAG+GGG 0.684532 8.3:-22165569 MS.gene011713:CDS
GTTCCACCAGAAATTGTCAG+GGG 0.692592 8.3:+22167651 None:intergenic
GAGGTCCAAAGATTCTTCGG+AGG 0.716472 8.3:-22165636 MS.gene011713:CDS
AGCTGGAGTAGTCAAACACG+GGG 0.727088 8.3:-22168023 MS.gene011713:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTAATTGTTACCTATA+AGG - chr8.3:22166276-22166295 MS.gene011713:intron 15.0%
!! AAAAAATTCAGATTCAAACT+AGG + chr8.3:22166121-22166140 None:intergenic 20.0%
!! AATAAATTATTTGATCTTGC+TGG - chr8.3:22167506-22167525 MS.gene011713:intron 20.0%
!! CAATTCAAATAAAACTATCA+CGG + chr8.3:22167648-22167667 None:intergenic 20.0%
!! GTTGACAATGATAAATAAAT+TGG - chr8.3:22167548-22167567 MS.gene011713:intron 20.0%
!! TAAGCATGTTGAAAATATAA+GGG - chr8.3:22166244-22166263 MS.gene011713:intron 20.0%
!! TCTATTCATTTGTGAAAATT+TGG + chr8.3:22166091-22166110 None:intergenic 20.0%
!!! GTTTTTCTTATAGTTTTTCT+AGG + chr8.3:22167252-22167271 None:intergenic 20.0%
! AAGCATGTTGAAAATATAAG+GGG - chr8.3:22166245-22166264 MS.gene011713:intron 25.0%
! AATGCAAAATGCTTCATATT+TGG - chr8.3:22168070-22168089 MS.gene011713:CDS 25.0%
! AATTACTTTCAATGCAATGA+CGG + chr8.3:22166889-22166908 None:intergenic 25.0%
! ATATAAATGTCACAGCAATA+TGG - chr8.3:22165670-22165689 MS.gene011713:CDS 25.0%
! CAACTGAATATAAAATTACC+TGG + chr8.3:22167019-22167038 None:intergenic 25.0%
! CCCAACTAATAAACAAAATT+CGG - chr8.3:22167677-22167696 MS.gene011713:CDS 25.0%
! GTAAGCATGTTGAAAATATA+AGG - chr8.3:22166243-22166262 MS.gene011713:intron 25.0%
! TAAAAAAGATGGTAGAAACT+TGG - chr8.3:22167130-22167149 MS.gene011713:intron 25.0%
! TATAAATGTCACAGCAATAT+GGG - chr8.3:22165671-22165690 MS.gene011713:CDS 25.0%
! TGAAAATATAAGGGGAAAAA+AGG - chr8.3:22166253-22166272 MS.gene011713:intron 25.0%
! TTAAAAGTTACCTGTGATTT+AGG - chr8.3:22166798-22166817 MS.gene011713:intron 25.0%
!! AATTGGTTATTGTCAGTTTT+GGG + chr8.3:22165741-22165760 None:intergenic 25.0%
!! ACTTTTCATTTCATCTCTAT+AGG - chr8.3:22166914-22166933 MS.gene011713:CDS 25.0%
!! ATGAAACACTTTAGTCTTTA+AGG + chr8.3:22167468-22167487 None:intergenic 25.0%
!! CAGGTAATTTTATATTCAGT+TGG - chr8.3:22167017-22167036 MS.gene011713:intron 25.0%
!! TATCTTTATGTATTTCTGCT+AGG + chr8.3:22165557-22165576 None:intergenic 25.0%
!!! AATAACTTTCTAAGATTTGG+TGG + chr8.3:22166954-22166973 None:intergenic 25.0%
!!! ACCGAATTTTGTTTATTAGT+TGG + chr8.3:22167681-22167700 None:intergenic 25.0%
!!! CCGAATTTTGTTTATTAGTT+GGG + chr8.3:22167680-22167699 None:intergenic 25.0%
!!! GACAATAACTTTCTAAGATT+TGG + chr8.3:22166957-22166976 None:intergenic 25.0%
!!! TGATTGCATTATGCTTTTTT+GGG + chr8.3:22166738-22166757 None:intergenic 25.0%
ACTAGACCTAATTACTCATA+AGG + chr8.3:22166042-22166061 None:intergenic 30.0%
AGAAATTATCTCCATGATTG+TGG - chr8.3:22165953-22165972 MS.gene011713:intron 30.0%
AGGGATATGATAGAACATTA+CGG - chr8.3:22167306-22167325 MS.gene011713:intron 30.0%
ATAAATGTCACAGCAATATG+GGG - chr8.3:22165672-22165691 MS.gene011713:CDS 30.0%
ATGTTCCACTACAAATAGTT+AGG + chr8.3:22165851-22165870 None:intergenic 30.0%
ATTACTTTCAATGCAATGAC+GGG + chr8.3:22166888-22166907 None:intergenic 30.0%
ATTGACCATGTCTATTACAA+AGG + chr8.3:22167424-22167443 None:intergenic 30.0%
ATTGTTACCTATAAGGAGTA+AGG - chr8.3:22166283-22166302 MS.gene011713:intron 30.0%
CAAAACTGACAATAACCAAT+TGG - chr8.3:22165740-22165759 MS.gene011713:intron 30.0%
CTAGACCTAATTACTCATAA+GGG + chr8.3:22166041-22166060 None:intergenic 30.0%
GCTTAGGATGTATTAAATTG+CGG + chr8.3:22166479-22166498 None:intergenic 30.0%
GGTATGTGATAAGTTTATCA+TGG - chr8.3:22167698-22167717 MS.gene011713:CDS 30.0%
GTAAACCTTTGTAATAGACA+TGG - chr8.3:22167416-22167435 MS.gene011713:intron 30.0%
GTTCAAAAGAACAAGTATCA+AGG - chr8.3:22165883-22165902 MS.gene011713:intron 30.0%
TAGACCTAATTACTCATAAG+GGG + chr8.3:22166040-22166059 None:intergenic 30.0%
TGGAAGTAATGAACAGAAAA+TGG - chr8.3:22167526-22167545 MS.gene011713:intron 30.0%
TGTATTGTGAACAGGATAAA+TGG - chr8.3:22165643-22165662 MS.gene011713:CDS 30.0%
TGTTCCACTACAAATAGTTA+GGG + chr8.3:22165850-22165869 None:intergenic 30.0%
TTGTTACCTATAAGGAGTAA+GGG - chr8.3:22166284-22166303 MS.gene011713:intron 30.0%
TTTATATTCAGTTGGATGTG+TGG - chr8.3:22167025-22167044 MS.gene011713:intron 30.0%
TTTCTTATCACAAGCATACA+AGG + chr8.3:22166453-22166472 None:intergenic 30.0%
! ATTGGTTATTGTCAGTTTTG+GGG + chr8.3:22165740-22165759 None:intergenic 30.0%
! CAATTGGTTATTGTCAGTTT+TGG + chr8.3:22165742-22165761 None:intergenic 30.0%
! GATACTTGTTCTTTTGAACT+AGG + chr8.3:22165882-22165901 None:intergenic 30.0%
! TGAGGTACTACAAAAAATGA+AGG + chr8.3:22166210-22166229 None:intergenic 30.0%
! TTATTTTCCCCTCTTTACAA+TGG - chr8.3:22166708-22166727 MS.gene011713:intron 30.0%
!!! CTGATTGCATTATGCTTTTT+TGG + chr8.3:22166739-22166758 None:intergenic 30.0%
AATAGACATGGTCAATGATC+TGG - chr8.3:22167428-22167447 MS.gene011713:intron 35.0%
ACGTTGAACTACTTTACTCA+AGG + chr8.3:22165766-22165785 None:intergenic 35.0%
AGAGGCTATTGTCAATATGA+AGG - chr8.3:22166567-22166586 MS.gene011713:intron 35.0%
AGATCTAGAGATCAATGAGT+TGG - chr8.3:22167281-22167300 MS.gene011713:intron 35.0%
AGTACAAGTAGATCAGATGA+AGG - chr8.3:22167197-22167216 MS.gene011713:intron 35.0%
ATTGTGAACAGGATAAATGG+AGG - chr8.3:22165646-22165665 MS.gene011713:CDS 35.0%
CAAAATTATGCCAAGTTCAG+CGG - chr8.3:22167756-22167775 MS.gene011713:CDS 35.0%
CCTTTCAAAATCCCAAATTG+AGG - chr8.3:22167867-22167886 MS.gene011713:intron 35.0%
GGGAGACAATAATGTATTAG+AGG - chr8.3:22166549-22166568 MS.gene011713:intron 35.0%
GTACAAGTAGATCAGATGAA+GGG - chr8.3:22167198-22167217 MS.gene011713:intron 35.0%
GTATGCTTTGTATTGTGAAC+AGG - chr8.3:22165635-22165654 MS.gene011713:CDS 35.0%
GTTCCACTACAAATAGTTAG+GGG + chr8.3:22165849-22165868 None:intergenic 35.0%
TAAGATATGCAGCAAATGCA+TGG + chr8.3:22166775-22166794 None:intergenic 35.0%
TACACCCCTTATGAGTAATT+AGG - chr8.3:22166033-22166052 MS.gene011713:intron 35.0%
TACCTCTATGTAAGAAGCAA+TGG + chr8.3:22166617-22166636 None:intergenic 35.0%
TCAAGATTAAGATGCAGCTT+AGG + chr8.3:22166495-22166514 None:intergenic 35.0%
TCATTTATAGCACCTTTGTG+AGG - chr8.3:22166173-22166192 MS.gene011713:intron 35.0%
TGTAAAGAGGGGAAAATAAC+AGG + chr8.3:22166707-22166726 None:intergenic 35.0%
TTACTTTCAATGCAATGACG+GGG + chr8.3:22166887-22166906 None:intergenic 35.0%
! AAGCATTGTATTGCTAGTAG+TGG + chr8.3:22166853-22166872 None:intergenic 35.0%
! AGAGATCAATGAGTTGGATA+GGG - chr8.3:22167287-22167306 MS.gene011713:intron 35.0%
! TAGAGATCAATGAGTTGGAT+AGG - chr8.3:22167286-22167305 MS.gene011713:intron 35.0%
!! AAGCTTTATTCCACTAAGTG+GGG + chr8.3:22167355-22167374 None:intergenic 35.0%
!! ACAAGCTTTATTCCACTAAG+TGG + chr8.3:22167357-22167376 None:intergenic 35.0%
!! CAAGCTTTATTCCACTAAGT+GGG + chr8.3:22167356-22167375 None:intergenic 35.0%
!! CACAAAGGTGCTATAAATGA+AGG + chr8.3:22166173-22166192 None:intergenic 35.0%
!! CCTCAATTTGGGATTTTGAA+AGG + chr8.3:22167870-22167889 None:intergenic 35.0%
!! TTGGTTATTGTCAGTTTTGG+GGG + chr8.3:22165739-22165758 None:intergenic 35.0%
!!! TCTACCATCTTTTTTACGAC+AGG + chr8.3:22167126-22167145 None:intergenic 35.0%
AAAATGGCGCACAATACTCA+TGG - chr8.3:22165462-22165481 MS.gene011713:CDS 40.0%
AAACTTGGCTTAGGTGGTTT+GGG - chr8.3:22167145-22167164 MS.gene011713:intron 40.0%
AAAGCTGGAGTAGTCAAACA+CGG - chr8.3:22165433-22165452 MS.gene011713:CDS 40.0%
AACGAGGTCCAAAGATTCTT+CGG - chr8.3:22167819-22167838 MS.gene011713:intron 40.0%
AAGCCCCTAACTATTTGTAG+TGG - chr8.3:22165843-22165862 MS.gene011713:intron 40.0%
AAGGGTTGTCAAATCACTAG+AGG - chr8.3:22167216-22167235 MS.gene011713:intron 40.0%
AGACCTTGTTAACTGTGCAA+TGG - chr8.3:22166341-22166360 MS.gene011713:intron 40.0%
AGATGGTAGAAACTTGGCTT+AGG - chr8.3:22167136-22167155 MS.gene011713:intron 40.0%
AGCACCTGTCGTAAAAAAGA+TGG - chr8.3:22167119-22167138 MS.gene011713:intron 40.0%
AGTTACCTGTGATTTAGGCT+TGG - chr8.3:22166803-22166822 MS.gene011713:intron 40.0%
ATGTCTTAATGATGGCAGGT+TGG - chr8.3:22166528-22166547 MS.gene011713:intron 40.0%
ATGTTCCACCAGAAATTGTC+AGG + chr8.3:22165812-22165831 None:intergenic 40.0%
ATTAAGCCCCTGACAATTTC+TGG - chr8.3:22165801-22165820 MS.gene011713:intron 40.0%
CTACAAATAGTTAGGGGCTT+AGG + chr8.3:22165843-22165862 None:intergenic 40.0%
CTACTTTACTCAAGGCCAAT+TGG + chr8.3:22165758-22165777 None:intergenic 40.0%
CTTACAGCAAAACTGCTATG+AGG + chr8.3:22166228-22166247 None:intergenic 40.0%
GAACCATTGCACAGTTAACA+AGG + chr8.3:22166347-22166366 None:intergenic 40.0%
GCAATGACATTTACGTTGAC+AGG - chr8.3:22167720-22167739 MS.gene011713:CDS 40.0%
GCCATCATTAAGACATGCAA+TGG + chr8.3:22166524-22166543 None:intergenic 40.0%
GCCATTGCATGTCTTAATGA+TGG - chr8.3:22166520-22166539 MS.gene011713:CDS 40.0%
GTCAATATGAAGGAGCCAAA+GGG - chr8.3:22166577-22166596 MS.gene011713:intron 40.0%
TACCTATAAGGAGTAAGGGA+AGG - chr8.3:22166288-22166307 MS.gene011713:intron 40.0%
TGCCATTGCTTCTTACATAG+AGG - chr8.3:22166612-22166631 MS.gene011713:intron 40.0%
TGTCAATATGAAGGAGCCAA+AGG - chr8.3:22166576-22166595 MS.gene011713:intron 40.0%
TGTCTTAATGATGGCAGGTT+GGG - chr8.3:22166529-22166548 MS.gene011713:intron 40.0%
TGTTCCACCAGAAATTGTCA+GGG + chr8.3:22165811-22165830 None:intergenic 40.0%
TTCCTTCCCTTACTCCTTAT+AGG + chr8.3:22166293-22166312 None:intergenic 40.0%
TTGCATGTCTTAATGATGGC+AGG - chr8.3:22166524-22166543 MS.gene011713:CDS 40.0%
! CTTTTATGTGCAGTCTCACT+TGG - chr8.3:22166671-22166690 MS.gene011713:intron 40.0%
! TGATAGCTCTGCCTCAATTT+GGG + chr8.3:22167881-22167900 None:intergenic 40.0%
! TTGATAGCTCTGCCTCAATT+TGG + chr8.3:22167882-22167901 None:intergenic 40.0%
AAGCTCTTCTCTGATGCTTG+TGG - chr8.3:22167795-22167814 MS.gene011713:CDS 45.0%
AAGCTGGAGTAGTCAAACAC+GGG - chr8.3:22165434-22165453 MS.gene011713:CDS 45.0%
AGAGAGAGAGAGAGAGAATC+GGG - chr8.3:22167094-22167113 MS.gene011713:intron 45.0%
AGGCAGAGCTATCAAAAGTG+AGG - chr8.3:22167887-22167906 MS.gene011713:intron 45.0%
ATCTCCATGATTGTGGTGCT+TGG - chr8.3:22165960-22165979 MS.gene011713:intron 45.0%
ATGCTCCAAGCCTAAATCAC+AGG + chr8.3:22166811-22166830 None:intergenic 45.0%
ATTCGAACGCGTACGCAAAA+TGG + chr8.3:22165503-22165522 None:intergenic 45.0%
CAAACCAAGCACCACAATCA+TGG + chr8.3:22165967-22165986 None:intergenic 45.0%
CAGAGCTATCAAAAGTGAGG+GGG - chr8.3:22167890-22167909 MS.gene011713:intron 45.0%
CGCAAAATGGAACTGAACTC+AGG + chr8.3:22165490-22165509 None:intergenic 45.0%
CTTCACCTCCGAAGAATCTT+TGG + chr8.3:22167830-22167849 None:intergenic 45.0%
CTTTGCAAAAGCAGCGATGA+AGG - chr8.3:22168041-22168060 MS.gene011713:CDS 45.0%
GAAACTTGGCTTAGGTGGTT+TGG - chr8.3:22167144-22167163 MS.gene011713:intron 45.0%
GACAAGTGGCAAAATAGTCC+AGG - chr8.3:22166998-22167017 MS.gene011713:intron 45.0%
GAGAGAGAGAGAGAGAGAAT+CGG - chr8.3:22167093-22167112 MS.gene011713:intron 45.0%
GCAGAGCTATCAAAAGTGAG+GGG - chr8.3:22167889-22167908 MS.gene011713:intron 45.0%
GCGTTCGAATGTAGATCTCA+AGG - chr8.3:22165513-22165532 MS.gene011713:CDS 45.0%
GGCAGAGCTATCAAAAGTGA+GGG - chr8.3:22167888-22167907 MS.gene011713:intron 45.0%
GTCAGCAAAATTGCAGCAGA+TGG - chr8.3:22166974-22166993 MS.gene011713:intron 45.0%
GTTCCACCAGAAATTGTCAG+GGG + chr8.3:22165810-22165829 None:intergenic 45.0%
TCTGCAGTCCATTTCTGCTT+AGG + chr8.3:22165397-22165416 None:intergenic 45.0%
TGGTAGAAACTTGGCTTAGG+TGG - chr8.3:22167139-22167158 MS.gene011713:intron 45.0%
! ATGGACTGCAGAAGAAGAAG+CGG - chr8.3:22165405-22165424 MS.gene011713:CDS 45.0%
! TAGCACCTTTGTGAGGTATC+TGG - chr8.3:22166180-22166199 MS.gene011713:intron 45.0%
! TGGGTGTGCCATTGTAAAGA+GGG + chr8.3:22166719-22166738 None:intergenic 45.0%
! TTGGGTGTGCCATTGTAAAG+AGG + chr8.3:22166720-22166739 None:intergenic 45.0%
!!! TTATTGTCAGTTTTGGGGGC+TGG + chr8.3:22165735-22165754 None:intergenic 45.0%
ACTAAGTGGGGTCAGCTACA+TGG + chr8.3:22167343-22167362 None:intergenic 50.0%
AGCTGCAAAAGCAGTTGCAG+AGG - chr8.3:22167939-22167958 MS.gene011713:intron 50.0%
AGCTGGAGTAGTCAAACACG+GGG - chr8.3:22165435-22165454 MS.gene011713:CDS 50.0%
ATGTAGCTGACCCCACTTAG+TGG - chr8.3:22167342-22167361 MS.gene011713:intron 50.0%
CTCTGATGCTTGTGGAAACG+AGG - chr8.3:22167803-22167822 MS.gene011713:CDS 50.0%
GAGAGAGAGAGAGAGAATCG+GGG - chr8.3:22167095-22167114 MS.gene011713:intron 50.0%
GAGGTCCAAAGATTCTTCGG+AGG - chr8.3:22167822-22167841 MS.gene011713:intron 50.0%
GATCGCCAGATACCTCACAA+AGG + chr8.3:22166188-22166207 None:intergenic 50.0%
GATTGCAGAAGCTGAAGCTG+AGG - chr8.3:22168008-22168027 MS.gene011713:CDS 50.0%
TCAAACACGGGGCTGGAAAA+TGG - chr8.3:22165446-22165465 MS.gene011713:CDS 50.0%
TCACAGCAATATGGGGATCC+CGG - chr8.3:22165679-22165698 MS.gene011713:CDS 50.0%
TGGGTGCTCCTAAGCAGAAA+TGG - chr8.3:22165386-22165405 MS.gene011713:CDS 50.0%
TTCTCAGAGACCGCTGAACT+TGG + chr8.3:22167769-22167788 None:intergenic 50.0%
TTGCAGCAGATGGTGACAAG+TGG - chr8.3:22166984-22167003 MS.gene011713:intron 50.0%
! AAGCCCCTGACAATTTCTGG+TGG - chr8.3:22165804-22165823 MS.gene011713:intron 50.0%
! GGCAAGTTTTGCTTTCTGCC+GGG + chr8.3:22165700-22165719 None:intergenic 50.0%
! GGGCAAGTTTTGCTTTCTGC+CGG + chr8.3:22165701-22165720 None:intergenic 50.0%
! GGGTGTGCCATTGTAAAGAG+GGG + chr8.3:22166718-22166737 None:intergenic 50.0%
! TCAGCGGTCTCTGAGAAAAG+AGG - chr8.3:22167772-22167791 MS.gene011713:CDS 50.0%
GGCAGAAGTTGCCATTGCAC+AGG - chr8.3:22167960-22167979 MS.gene011713:CDS 55.0%
! GAAGAAGCGGCGTTGAAAGC+TGG - chr8.3:22165418-22165437 MS.gene011713:CDS 55.0%
! GCAGCCTTGTCAGAACCCTT+TGG + chr8.3:22166595-22166614 None:intergenic 55.0%
! GGAGCCAAAGGGTTCTGACA+AGG - chr8.3:22166588-22166607 MS.gene011713:intron 55.0%
!!! TGTCAGTTTTGGGGGCTGGA+AGG + chr8.3:22165731-22165750 None:intergenic 55.0%
ACAGGCCGAAGCAGCAGCTA+GGG - chr8.3:22167978-22167997 MS.gene011713:CDS 60.0%
GAGGGGGATGTCAGCTCAAG+AGG - chr8.3:22167906-22167925 MS.gene011713:intron 60.0%
GGAGTAGTCAAACACGGGGC+TGG - chr8.3:22165439-22165458 MS.gene011713:CDS 60.0%
GGGGATGTCAGCTCAAGAGG+CGG - chr8.3:22167909-22167928 MS.gene011713:intron 60.0%
GGGGGCTGGAAGGCTCTTAA+GGG + chr8.3:22165721-22165740 None:intergenic 60.0%
TGGGGGCTGGAAGGCTCTTA+AGG + chr8.3:22165722-22165741 None:intergenic 60.0%
! TGCTGCTTCGGCCTGTGCAA+TGG + chr8.3:22167974-22167993 None:intergenic 60.0%
CACAGGCCGAAGCAGCAGCT+AGG - chr8.3:22167977-22167996 MS.gene011713:CDS 65.0%
CGAAGCAGCAGCTAGGGAGG+CGG - chr8.3:22167984-22168003 MS.gene011713:CDS 65.0%
! CGCCTCCCTAGCTGCTGCTT+CGG + chr8.3:22167986-22168005 None:intergenic 65.0%
GGCCGAAGCAGCAGCTAGGG+AGG - chr8.3:22167981-22168000 MS.gene011713:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 22165385 22168095 22165385 ID=MS.gene011713
chr8.3 mRNA 22165385 22168095 22165385 ID=MS.gene011713.t1;Parent=MS.gene011713
chr8.3 exon 22167946 22168095 22167946 ID=MS.gene011713.t1.exon1;Parent=MS.gene011713.t1
chr8.3 CDS 22167946 22168095 22167946 ID=cds.MS.gene011713.t1;Parent=MS.gene011713.t1
chr8.3 exon 22167576 22167823 22167576 ID=MS.gene011713.t1.exon2;Parent=MS.gene011713.t1
chr8.3 CDS 22167576 22167823 22167576 ID=cds.MS.gene011713.t1;Parent=MS.gene011713.t1
chr8.3 exon 22166847 22166934 22166847 ID=MS.gene011713.t1.exon3;Parent=MS.gene011713.t1
chr8.3 CDS 22166847 22166934 22166847 ID=cds.MS.gene011713.t1;Parent=MS.gene011713.t1
chr8.3 exon 22166461 22166544 22166461 ID=MS.gene011713.t1.exon4;Parent=MS.gene011713.t1
chr8.3 CDS 22166461 22166544 22166461 ID=cds.MS.gene011713.t1;Parent=MS.gene011713.t1
chr8.3 exon 22165385 22165738 22165385 ID=MS.gene011713.t1.exon5;Parent=MS.gene011713.t1
chr8.3 CDS 22165385 22165738 22165385 ID=cds.MS.gene011713.t1;Parent=MS.gene011713.t1
Gene Sequence

>MS.gene011713

ATGGGTGCTCCTAAGCAGAAATGGACTGCAGAAGAAGAAGCGGCGTTGAAAGCTGGAGTAGTCAAACACGGGGCTGGAAAATGGCGCACAATACTCATGGATCCTGAGTTCAGTTCCATTTTGCGTACGCGTTCGAATGTAGATCTCAAGGTAAGTCTTTTCAACACAGCCTAGCAGAAATACATAAAGATAGATAATTTTTCTTTGATCAAATTGATCAAGCTATCGCATACTTTTAGTGAACTAATTTGTATGCTTTGTATTGTGAACAGGATAAATGGAGGAATATAAATGTCACAGCAATATGGGGATCCCGGCAGAAAGCAAAACTTGCCCTTAAGAGCCTTCCAGCCCCCAAAACTGACAATAACCAATTGGCCTTGAGTAAAGTAGTTCAACGTGAAGACTTTCTCGATATTAAGCCCCTGACAATTTCTGGTGGAACATTGCAATCTCCTAAGCCCCTAACTATTTGTAGTGGAACATTGCAATCTCCTAGTTCAAAAGAACAAGTATCAAGGTTCAAGAACTTTCAGTTAAATACATCTGCTATTATTATCTAGCGAAAAGAAATTATCTCCATGATTGTGGTGCTTGGTTTGTGATTTTGTTCTTAAATAACATCTAAAGATTGTAGAGTGTAAATGTTACACCCCTTATGAGTAATTAGGTCTAGTATTGCATAAATTTTTGTGCTAGTTTACCAAATTTTCACAAATGAATAGATAAATAGCCTAGTTTGAATCTGAATTTTTTGTTATTTTCTACGTGATTTATCTTGTGATCCTTCATTTATAGCACCTTTGTGAGGTATCTGGCGATCCTTCATTTTTTGTAGTACCTCATAGCAGTTTTGCTGTAAGCATGTTGAAAATATAAGGGGAAAAAAGGTTTATTAATTGTTACCTATAAGGAGTAAGGGAAGGAAAAAAAAAAAGAAAGAATAGAAAGATCATAGACCTTGTTAACTGTGCAATGGTTCATTGCATTTGAATGAAGTTATATGACTCATACTTTTATTTATTTACTTATTTTTAATTATATCATTCTCTGTCTACGTGCACTCCTTGTATGCTTGTGATAAGAAAATACCGCAATTTAATACATCCTAAGCTGCATCTTAATCTTGACATATGCCATTGCATGTCTTAATGATGGCAGGTTGGGAGACAATAATGTATTAGAGGCTATTGTCAATATGAAGGAGCCAAAGGGTTCTGACAAGGCTGCCATTGCTTCTTACATAGAGGTATTTCTATGTCGCGTTGTGTGTTTGTGTGTTAAAGCTTTTATGTGCAGTCTCACTTGGCAAGTAAAAACCTGTTATTTTCCCCTCTTTACAATGGCACACCCAAAAAAGCATAATGCAATCAGTCATAGTATCATACCATGCATTTGCTGCATATCTTATCTTTAAAAGTTACCTGTGATTTAGGCTTGGAGCATTAGTGTATAGTCAATTCAGCCACTACTAGCAATACAATGCTTGTTTACATTAACCCCGTCATTGCATTGAAAGTAATTCTGAAACTTTTCATTTCATCTCTATAGGAAAAATATCAGTGTCCACCAAATCTTAGAAAGTTATTGTCAGCAAAATTGCAGCAGATGGTGACAAGTGGCAAAATAGTCCAGGTAATTTTATATTCAGTTGGATGTGTGGTAAGACTAGACAAGATAGAATTAGAAATACAACATTAGAGAGAGAGAGAGAGAGAGAGAGAGAATCGGGGTAGCACCTGTCGTAAAAAAGATGGTAGAAACTTGGCTTAGGTGGTTTGGGCATGTTGAGAGAAGACTTGTATATTCTATAGTACAAGTAGATCAGATGAAGGGTTGTCAAATCACTAGAGGCAAATAAAGACCTAGAAAAACTATAAGAAAAACTGTTAAGAAAGATCTAGAGATCAATGAGTTGGATAGGGATATGATAGAACATTACGGCGTCGTTTGATCCATGTAGCTGACCCCACTTAGTGGAATAAAGCTTGTTTGTTCTTGTGATTGTCATTGTTACATTAAGCTTTATGTGTAAACCTTTGTAATAGACATGGTCAATGATCTGGATAATATTTGAAGACCTTAAAGACTAAAGTGTTTCATCTATGACAGAATTACTATAATAAATTATTTGATCTTGCTGGAAGTAATGAACAGAAAATGGTTGACAATGATAAATAAATTGGTTATAACATTATGTTTAGATGCGTTTTTCGTCAAACAAAAAAAAAAAAGATACGTGTTCTCTTTTGCAGTAGTACCGTGATAGTTTTATTTGAATTGATAGATTGTCCCAACTAATAAACAAAATTCGGTATGTGATAAGTTTATCATGGCAATGACATTTACGTTGACAGGAAAAGCACAAGTACAAAATTATGCCAAGTTCAGCGGTCTCTGAGAAAAGAGGAAGCTCTTCTCTGATGCTTGTGGAAACGAGGTCCAAAGATTCTTCGGAGGTGAAGATGTGTGATGTCAACATCCTTTCAAAATCCCAAATTGAGGCAGAGCTATCAAAAGTGAGGGGGATGTCAGCTCAAGAGGCGGCTGCTGCAGCTGCAAAAGCAGTTGCAGAGGCAGAAGTTGCCATTGCACAGGCCGAAGCAGCAGCTAGGGAGGCGGAGATTGCAGAAGCTGAAGCTGAGGCTGCTCAAGTCTTTGCAAAAGCAGCGATGAAGGCACTCAAATGCAAAATGCTTCATATTTGGTAG
Protein sequence

>MS.gene011713.t1

MGAPKQKWTAEEEAALKAGVVKHGAGKWRTILMDPEFSSILRTRSNVDLKDKWRNINVTAIWGSRQKAKLALKSLPAPKTDNNQLALSKVVQREDFLDIKPLTISGGTLQSPKPLTICSGTLQSPSSKEQVSRLGDNNVLEAIVNMKEPKGSDKAAIASYIEEKYQCPPNLRKLLSAKLQQMVTSGKIVQEKHKYKIMPSSAVSEKRGSSSLMLVETRSKDSSEVKMCDVNILSKSQIEAELSKVRGMSAQEAAAAAAKAVAEAEVAIAQAEAAAREAEIAEAEAEAAQVFAKAAMKALKCKMLHIW