AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011717.t1 MTR_4g097080 96.863 510 10 3 1 510 1 504 0.0 1023
MS.gene011717.t1 MTR_5g015490 68.008 522 124 14 1 510 1 491 0.0 645
MS.gene011717.t1 MTR_2g099110 52.758 417 170 9 107 510 50 452 3.50e-143 419
MS.gene011717.t1 MTR_2g082090 31.937 382 239 6 135 508 210 578 1.85e-59 206
MS.gene011717.t1 MTR_2g089100 29.004 462 275 9 81 510 4 444 2.09e-59 203
MS.gene011717.t1 MTR_5g094450 30.366 382 251 5 135 509 159 532 5.84e-59 204
MS.gene011717.t1 MTR_5g097480 30.469 384 244 7 135 509 175 544 1.35e-58 203
MS.gene011717.t1 MTR_1g029420 31.088 386 239 8 135 509 223 592 5.14e-58 202
MS.gene011717.t1 MTR_3g056110 29.397 398 250 9 125 509 163 542 1.53e-54 192
MS.gene011717.t1 MTR_3g089055 29.737 380 252 6 135 509 196 565 2.28e-53 189
MS.gene011717.t1 MTR_3g089055 29.737 380 252 6 135 509 201 570 2.29e-53 189
MS.gene011717.t1 MTR_4g095500 28.033 478 286 11 80 510 3 469 3.77e-53 187
MS.gene011717.t1 MTR_5g015950 28.176 433 254 9 126 510 24 447 6.76e-52 182
MS.gene011717.t1 MTR_3g053270 28.176 433 254 9 126 510 24 447 6.76e-52 182
MS.gene011717.t1 MTR_7g027190 29.873 395 236 10 136 509 299 673 6.61e-51 184
MS.gene011717.t1 MTR_4g133660 30.570 386 240 10 135 508 173 542 5.02e-50 180
MS.gene011717.t1 MTR_8g020840 32.494 397 233 13 133 509 167 548 2.74e-49 178
MS.gene011717.t1 MTR_2g026250 29.494 356 236 6 159 509 251 596 9.81e-49 177
MS.gene011717.t1 MTR_4g102790 26.478 389 256 8 136 510 291 663 1.20e-42 161
MS.gene011717.t1 MTR_7g074650 28.191 376 242 11 140 510 441 793 1.45e-42 162
MS.gene011717.t1 MTR_4g077760 29.443 377 246 8 138 509 194 555 2.16e-42 159
MS.gene011717.t1 MTR_3g065980 26.943 386 250 7 138 509 173 540 1.03e-40 154
MS.gene011717.t1 MTR_2g097410 29.921 381 251 8 137 510 371 742 4.54e-40 154
MS.gene011717.t1 MTR_4g064200 28.346 381 253 10 137 510 284 651 9.45e-40 153
MS.gene011717.t1 MTR_8g442410 28.796 382 250 12 138 509 156 525 4.63e-39 149
MS.gene011717.t1 MTR_2g097310 26.579 380 261 9 137 510 272 639 5.21e-39 150
MS.gene011717.t1 MTR_4g064150 27.273 385 262 7 135 510 359 734 1.06e-38 150
MS.gene011717.t1 MTR_4g064160 26.963 382 263 7 135 510 314 685 2.13e-38 149
MS.gene011717.t1 MTR_4g064120 27.937 383 253 10 135 506 252 622 2.63e-38 148
MS.gene011717.t1 MTR_2g097350 25.984 381 262 9 137 510 274 641 6.64e-38 147
MS.gene011717.t1 MTR_7g062120 24.409 381 274 5 135 510 370 741 4.31e-37 145
MS.gene011717.t1 MTR_4g064180 27.937 383 253 10 135 506 252 622 1.09e-36 144
MS.gene011717.t1 MTR_4g104020 25.831 391 253 10 137 509 149 520 7.77e-35 137
MS.gene011717.t1 MTR_3g022830 23.888 427 254 10 138 509 26 436 5.58e-34 133
MS.gene011717.t1 MTR_2g097390 25.326 383 262 9 137 510 275 642 7.49e-34 135
MS.gene011717.t1 MTR_2g097467 24.672 381 266 8 137 510 289 655 1.67e-33 134
MS.gene011717.t1 MTR_4g076020 26.842 380 233 14 140 510 87 430 2.23e-33 132
MS.gene011717.t1 MTR_1g069725 27.441 379 243 12 137 508 109 462 2.94e-32 129
MS.gene011717.t1 MTR_2g097463 25.594 379 265 8 137 510 290 656 1.92e-31 129
MS.gene011717.t1 MTR_2g097473 24.802 379 268 7 137 510 289 655 3.41e-31 128
MS.gene011717.t1 MTR_3g072710 26.735 389 261 11 136 508 119 499 4.09e-29 120
MS.gene011717.t1 MTR_7g057230 25.995 427 267 11 103 508 258 656 5.59e-28 118
MS.gene011717.t1 MTR_1g096030 25.686 401 251 13 136 509 79 459 1.06e-27 115
MS.gene011717.t1 MTR_2g034260 23.940 401 284 7 124 509 190 584 1.90e-27 116
MS.gene011717.t1 MTR_2g097380 26.053 380 228 13 140 510 227 562 4.33e-27 115
MS.gene011717.t1 MTR_5g058860 26.992 389 258 12 136 508 118 496 1.41e-26 113
MS.gene011717.t1 MTR_6g047750 25.505 396 250 12 134 509 292 662 3.21e-26 113
MS.gene011717.t1 MTR_2g034250 23.632 402 285 8 124 509 190 585 9.01e-26 111
MS.gene011717.t1 MTR_4g076140 31.776 214 134 3 136 347 47 250 7.87e-25 107
MS.gene011717.t1 MTR_5g009080 31.122 196 111 4 160 347 79 258 9.53e-25 107
MS.gene011717.t1 MTR_5g009080 30.323 155 99 4 357 509 331 478 1.71e-12 70.1
MS.gene011717.t1 MTR_2g034280 24.318 403 278 9 124 509 183 575 4.96e-24 105
MS.gene011717.t1 MTR_1g086970 25.000 360 249 9 159 509 131 478 7.25e-24 104
MS.gene011717.t1 MTR_8g093070 26.873 387 244 14 136 510 138 497 1.17e-23 104
MS.gene011717.t1 MTR_7g109580 22.616 451 325 9 67 509 120 554 5.27e-23 102
MS.gene011717.t1 MTR_4g026485 22.691 379 262 13 138 509 270 624 7.74e-20 93.2
MS.gene011717.t1 MTR_8g077940 23.684 456 316 13 63 509 109 541 3.85e-17 84.7
MS.gene011717.t1 MTR_0092s0100 21.883 393 274 12 127 509 362 731 5.63e-17 84.7
MS.gene011717.t1 MTR_0092s0100 21.883 393 274 12 127 509 359 728 6.35e-17 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011717.t1 AT4G37650 57.353 544 184 13 1 510 1 530 0.0 549
MS.gene011717.t1 AT2G04890 31.746 378 236 7 137 509 53 413 1.87e-61 207
MS.gene011717.t1 AT1G50600 29.439 428 276 8 91 509 116 526 2.98e-58 202
MS.gene011717.t1 AT1G50600 29.673 428 275 9 91 509 187 597 6.71e-58 202
MS.gene011717.t1 AT1G50600 29.439 428 276 8 91 509 191 601 9.21e-58 202
MS.gene011717.t1 AT3G49950 33.249 394 233 10 135 510 28 409 3.29e-56 193
MS.gene011717.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.94e-54 190
MS.gene011717.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.94e-54 190
MS.gene011717.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.94e-54 190
MS.gene011717.t1 AT5G48150 28.382 377 256 5 138 509 123 490 2.94e-54 190
MS.gene011717.t1 AT1G21450 31.328 399 238 9 127 509 215 593 8.14e-54 191
MS.gene011717.t1 AT1G66350 31.117 376 230 10 138 509 156 506 2.62e-48 174
MS.gene011717.t1 AT3G03450 28.684 380 245 7 138 509 184 545 3.34e-47 172
MS.gene011717.t1 AT4G17230 27.344 384 258 6 135 510 156 526 1.15e-45 167
MS.gene011717.t1 AT4G17230 27.344 384 258 6 135 510 156 526 1.15e-45 167
MS.gene011717.t1 AT5G17490 29.101 378 240 7 138 509 161 516 6.84e-45 165
MS.gene011717.t1 AT3G54220 29.024 379 238 12 140 510 296 651 1.58e-42 160
MS.gene011717.t1 AT1G14920 28.533 375 247 8 138 509 173 529 3.01e-42 158
MS.gene011717.t1 AT2G01570 27.467 375 251 8 138 509 225 581 9.17e-42 158
MS.gene011717.t1 AT2G29060 28.640 419 268 12 109 510 252 656 1.77e-41 158
MS.gene011717.t1 AT2G29060 28.640 419 268 12 109 510 289 693 2.37e-41 158
MS.gene011717.t1 AT3G13840 30.928 388 236 13 133 509 144 510 5.75e-41 154
MS.gene011717.t1 AT5G66770 29.189 370 245 8 145 509 227 584 3.56e-40 153
MS.gene011717.t1 AT2G29065 25.907 386 270 8 135 510 250 629 3.26e-38 148
MS.gene011717.t1 AT1G07530 26.371 383 265 8 135 510 394 766 4.71e-38 149
MS.gene011717.t1 AT2G37650 25.984 381 266 7 135 510 333 702 1.11e-37 147
MS.gene011717.t1 AT2G37650 25.984 381 266 7 135 510 345 714 1.26e-37 147
MS.gene011717.t1 AT1G50420 25.792 442 242 8 136 509 56 479 2.79e-37 144
MS.gene011717.t1 AT1G63100 27.506 389 255 9 136 508 276 653 4.47e-36 142
MS.gene011717.t1 AT1G63100 27.506 389 255 9 136 508 251 628 4.86e-36 142
MS.gene011717.t1 AT3G46600 26.440 382 264 6 135 510 2 372 6.50e-36 137
MS.gene011717.t1 AT3G46600 26.440 382 264 6 135 510 210 580 4.54e-35 139
MS.gene011717.t1 AT3G46600 26.440 382 264 6 135 510 178 548 8.76e-35 137
MS.gene011717.t1 AT5G59450 24.655 507 339 11 21 510 119 599 1.43e-33 134
MS.gene011717.t1 AT3G50650 29.248 359 231 9 160 509 198 542 2.79e-33 133
MS.gene011717.t1 AT5G41920 27.676 383 244 13 136 509 42 400 2.64e-32 128
MS.gene011717.t1 AT5G52510 24.874 398 255 9 134 509 265 640 3.15e-32 131
MS.gene011717.t1 AT1G55580 29.017 417 237 14 137 509 44 445 5.10e-32 128
MS.gene011717.t1 AT1G07520 26.478 389 263 10 135 510 316 694 1.03e-31 129
MS.gene011717.t1 AT1G07520 26.478 389 263 10 135 510 317 695 1.23e-31 129
MS.gene011717.t1 AT1G07520 26.478 389 263 10 135 510 152 530 2.00e-31 127
MS.gene011717.t1 AT1G07520 26.478 389 263 10 135 510 152 530 2.00e-31 127
MS.gene011717.t1 AT4G08250 28.859 298 198 8 217 508 187 476 3.93e-26 111
MS.gene011717.t1 AT3G60630 25.497 302 204 9 217 509 333 622 5.07e-15 78.2
MS.gene011717.t1 AT4G00150 24.282 383 241 15 139 509 209 554 1.59e-14 76.6
MS.gene011717.t1 AT4G36710 22.038 422 286 14 96 509 99 485 6.44e-12 68.2
MS.gene011717.t1 AT2G45160 23.362 351 230 12 172 509 315 639 1.61e-11 67.4

Find 120 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCAATTATGTGAAGTTT+AGG 0.248165 8.3:-22140337 None:intergenic
AAACAAATCACGGTGGTTTC+CGG 0.257711 8.3:-22140659 None:intergenic
AAGAGTGTTTAAGATGGTTT+AGG 0.258495 8.3:+22140782 MS.gene011717:CDS
TATCAATTATGTGAAGTTTA+GGG 0.283325 8.3:-22140336 None:intergenic
ATTCTTCGTCTTCTTCTTGT+TGG 0.301725 8.3:-22139754 None:intergenic
AGGAGGAGGTTGCATGGTGG+TGG 0.328666 8.3:+22140952 MS.gene011717:CDS
TTGTCCATGGACTAACTTCT+TGG 0.329314 8.3:-22140261 None:intergenic
TAAGTAGTGTTTGTTGTGTT+TGG 0.339821 8.3:-22139896 None:intergenic
ATGAAGTTTGTGATGATGTT+AGG 0.340214 8.3:+22140998 MS.gene011717:CDS
GCTGATTTAGAAGTTTGTTT+TGG 0.347659 8.3:+22140736 MS.gene011717:CDS
AGAGTGTTTAAGATGGTTTA+GGG 0.373474 8.3:+22140783 MS.gene011717:CDS
TGGAAGAGGTATTGTTGATT+TGG 0.374691 8.3:+22140876 MS.gene011717:CDS
ATCACGGTGGTTTCCGGCTC+CGG 0.383080 8.3:-22140653 None:intergenic
GTTAAATGGATACGTTGTTT+AGG 0.388354 8.3:+22139606 None:intergenic
AACTCTTTACATTCGATATC+CGG 0.397888 8.3:+22140634 MS.gene011717:CDS
TCCACATGAGTTGTTGGATT+CGG 0.400997 8.3:-22140063 None:intergenic
TACTTCAACTTTGAATTCTC+CGG 0.412778 8.3:+22139977 MS.gene011717:CDS
GTTAGTCCATGGACAACGTT+TGG 0.420735 8.3:+22140268 MS.gene011717:CDS
AAAATGCACGTGCGGTTTCA+AGG 0.430548 8.3:-22140024 None:intergenic
CGGTGGTGTGGGCCAGTGTA+TGG 0.430711 8.3:+22141112 MS.gene011717:CDS
CTTCCACGACAAGAATATTC+CGG 0.431546 8.3:-22141082 None:intergenic
GCATGGTGGTGGGTTTAATA+CGG 0.431980 8.3:+22140963 MS.gene011717:CDS
GGAGGAGGTTGCATGGTGGT+GGG 0.435450 8.3:+22140953 MS.gene011717:CDS
TATTAAAGAAGATGAAGCTT+TGG 0.437246 8.3:+22140597 MS.gene011717:CDS
TGTTTATATCTTTGCTTCGT+GGG 0.441689 8.3:+22140677 MS.gene011717:CDS
TTAGCATCCACATGAGTTGT+TGG 0.445709 8.3:-22140069 None:intergenic
TTGTTTATATCTTTGCTTCG+TGG 0.451946 8.3:+22140676 MS.gene011717:CDS
TGCTTCGTGGGTTAGAACCT+AGG 0.461690 8.3:+22140689 MS.gene011717:CDS
CGTCTCTCCATTGACTCATA+AGG 0.462546 8.3:-22140910 None:intergenic
CAGCGTCACGGTGGAGGAGG+AGG 0.463327 8.3:+22140938 MS.gene011717:CDS
TTTGGTTCGGATTTCGTGGA+AGG 0.470121 8.3:+22140754 MS.gene011717:CDS
GATTCGGTTTGTGTTGTTGT+CGG 0.471253 8.3:-22140047 None:intergenic
GCTATCTTAGAAGCATTAGA+AGG 0.474912 8.3:+22140310 MS.gene011717:CDS
TAAGGTGGTGGTGGAGTTGA+AGG 0.476215 8.3:-22139678 None:intergenic
ATGTTAGGGCTTTGTTGAGA+AGG 0.482294 8.3:+22141013 MS.gene011717:CDS
CGGTGACTACAGTGGTTAAA+CGG 0.484162 8.3:-22140435 None:intergenic
GTGGAGTTGAAGGAAGGATC+AGG 0.484573 8.3:-22139668 None:intergenic
TGAAGTTTGTGATGATGTTA+GGG 0.489537 8.3:+22140999 MS.gene011717:CDS
AGAGCTGCTAGAAGTTGTTA+AGG 0.490994 8.3:-22139696 None:intergenic
TCATGAAGGAAATCGGTTCA+AGG 0.493998 8.3:+22140485 MS.gene011717:CDS
GCAACACTTATTGCACTCAA+TGG 0.496242 8.3:+22140362 MS.gene011717:CDS
TTGAATTCTCCGGCAACTCA+TGG 0.501115 8.3:+22139987 MS.gene011717:CDS
GCTTCGTGGGTTAGAACCTA+GGG 0.504275 8.3:+22140690 MS.gene011717:CDS
GTAATTATCAGTTGTGGTGG+GGG 0.506791 8.3:-22139933 None:intergenic
TTGATGCTAGAAAGAGAAGC+TGG 0.507592 8.3:+22140856 MS.gene011717:CDS
ATAGTAGTAGTGGATGCATA+AGG 0.510603 8.3:-22139866 None:intergenic
AGAGTAATTATCAGTTGTGG+TGG 0.511198 8.3:-22139936 None:intergenic
TGTTCCTCCGATGGTGATGC+CGG 0.512206 8.3:+22141063 MS.gene011717:CDS
GAGTAATTATCAGTTGTGGT+GGG 0.513221 8.3:-22139935 None:intergenic
TTTGATCTGTGTCACCGTAT+GGG 0.514015 8.3:-22140105 None:intergenic
CGGTGGAGGAGGAGGTTGCA+TGG 0.514673 8.3:+22140946 MS.gene011717:CDS
GAAGGATAAGCCGGTGGTGT+GGG 0.516813 8.3:+22141101 MS.gene011717:CDS
GGAAGGATAAGCCGGTGGTG+TGG 0.518070 8.3:+22141100 MS.gene011717:CDS
CGGAATATTCTTGTCGTGGA+AGG 0.518332 8.3:+22141083 MS.gene011717:CDS
CTAGAAAGAGAAGCTGGAAG+AGG 0.518793 8.3:+22140862 MS.gene011717:CDS
AAACAGCGTCACGGTGGAGG+AGG 0.519490 8.3:+22140935 MS.gene011717:CDS
CATACACTGGCCCACACCAC+CGG 0.526670 8.3:-22141111 None:intergenic
TTGATCTGTGTCACCGTATG+GGG 0.527505 8.3:-22140104 None:intergenic
TTGTTGAGAAGGTATAAAGA+AGG 0.530989 8.3:+22141024 MS.gene011717:CDS
AGAAGTTGTTAAGGTGGTGG+TGG 0.532155 8.3:-22139687 None:intergenic
ACCTCAAGGCCTCCATACAC+TGG 0.532917 8.3:-22141124 None:intergenic
TGCTTCTAAGATAGCACCGT+TGG 0.532927 8.3:-22140302 None:intergenic
GCCAACACTCCTCGAAGCGT+TGG 0.539050 8.3:+22140384 MS.gene011717:CDS
TGTTGAGAAGGTATAAAGAA+GGG 0.543842 8.3:+22141025 MS.gene011717:CDS
TTTAGTCGCATGAACGACGC+GGG 0.545808 8.3:+22140157 MS.gene011717:CDS
TCAACAACAGTTAAAACCCT+AGG 0.548008 8.3:-22140706 None:intergenic
CATCTTCCTCGTTGAATCGA+AGG 0.549319 8.3:-22140227 None:intergenic
ACGTGTCCAAACGTTGTCCA+TGG 0.550032 8.3:-22140274 None:intergenic
AGTAATTATCAGTTGTGGTG+GGG 0.552091 8.3:-22139934 None:intergenic
GAAGGAAATCGGTTCAAGGA+TGG 0.552770 8.3:+22140489 MS.gene011717:CDS
GCCAACGCTTCGAGGAGTGT+TGG 0.553618 8.3:-22140385 None:intergenic
AGCGGGTGGCCAACGCTTCG+AGG 0.554570 8.3:-22140393 None:intergenic
CACTGAGCCACCGCTGACTG+CGG 0.555174 8.3:-22140455 None:intergenic
TTGTGATCCTTATGAGTCAA+TGG 0.560449 8.3:+22140903 MS.gene011717:CDS
ATTCAAAGTTGAAGTAATCG+TGG 0.563080 8.3:-22139970 None:intergenic
TTGATATGTTGTTTGTCTTG+AGG 0.563090 8.3:-22139723 None:intergenic
GTGGTGGTGGAGTTGAAGGA+AGG 0.565220 8.3:-22139674 None:intergenic
TGGAGTAGTGATAGTAGTAG+TGG 0.565967 8.3:-22139876 None:intergenic
GCCAGTGTATGGAGGCCTTG+AGG 0.566308 8.3:+22141123 MS.gene011717:CDS
GTGAGGCGTATCATCAGAGC+GGG 0.570778 8.3:-22140410 None:intergenic
GTGAAACAGCGTCACGGTGG+AGG 0.572256 8.3:+22140932 MS.gene011717:CDS
GAATATTCCGGCATCACCAT+CGG 0.580354 8.3:-22141070 None:intergenic
TTTCAAGAACAAGTAGTGAG+AGG 0.582987 8.3:+22140833 MS.gene011717:CDS
AGTTCCAAGAAGTTAGTCCA+TGG 0.584447 8.3:+22140257 MS.gene011717:CDS
TTACATTCGATATCCGGAGC+CGG 0.585693 8.3:+22140640 MS.gene011717:CDS
ATGGACAACGTTTGGACACG+TGG 0.586613 8.3:+22140276 MS.gene011717:CDS
TTCAAAGTTGAAGTAATCGT+GGG 0.586851 8.3:-22139969 None:intergenic
ATGACACAGTGTTCCTCCGA+TGG 0.587322 8.3:+22141054 MS.gene011717:CDS
TTTGGACACGTGGCAGCCAA+CGG 0.587329 8.3:+22140286 MS.gene011717:CDS
CTTGTCGTGGAAGGATAAGC+CGG 0.587868 8.3:+22141092 MS.gene011717:CDS
GTTTAGTCGCATGAACGACG+CGG 0.592117 8.3:+22140156 MS.gene011717:CDS
GTGTTGTTTAGAAGAAGATG+AGG 0.594437 8.3:-22139831 None:intergenic
GAAAGAGTAATTATCAGTTG+TGG 0.594879 8.3:-22139939 None:intergenic
GCAAAGATATAAACAAATCA+CGG 0.596997 8.3:-22140669 None:intergenic
GTAGTCACCGCAGTCAGCGG+TGG 0.597985 8.3:+22140448 MS.gene011717:CDS
TGGTGTGGGCCAGTGTATGG+AGG 0.609272 8.3:+22141115 MS.gene011717:CDS
ATGCCGGAATATTCTTGTCG+TGG 0.614564 8.3:+22141079 MS.gene011717:CDS
ACCGAATCCAACAACTCATG+TGG 0.616002 8.3:+22140062 MS.gene011717:CDS
AACGAGCTTAGTTCCCCATA+CGG 0.616114 8.3:+22140091 MS.gene011717:CDS
GCTAGAAGTTGTTAAGGTGG+TGG 0.616229 8.3:-22139690 None:intergenic
AGGCGTATCATCAGAGCGGG+TGG 0.620961 8.3:-22140407 None:intergenic
AAGTTGAAGTAATCGTGGGA+GGG 0.628358 8.3:-22139965 None:intergenic
GCTGCTAGAAGTTGTTAAGG+TGG 0.637704 8.3:-22139693 None:intergenic
AAAGTTGAAGTAATCGTGGG+AGG 0.640378 8.3:-22139966 None:intergenic
TGAGAAGGTATAAAGAAGGG+TGG 0.652792 8.3:+22141028 MS.gene011717:CDS
ACTGTAGTCACCGCAGTCAG+CGG 0.657652 8.3:+22140445 MS.gene011717:CDS
ACAGTGGTTAAACGGAGGTG+AGG 0.666216 8.3:-22140427 None:intergenic
CAAAACATTAACATCCGCGT+CGG 0.669781 8.3:+22140192 MS.gene011717:CDS
GTCGTGGAAGGATAAGCCGG+TGG 0.674105 8.3:+22141095 MS.gene011717:CDS
TATTCCGGCATCACCATCGG+AGG 0.676733 8.3:-22141067 None:intergenic
CATGTTCCTTCGATTCAACG+AGG 0.690083 8.3:+22140221 MS.gene011717:CDS
TGGAGGAGGAGGTTGCATGG+TGG 0.690855 8.3:+22140949 MS.gene011717:CDS
GTTGTCGGAAAATGCACGTG+CGG 0.703618 8.3:-22140032 None:intergenic
GCTGACTGCGGTGACTACAG+TGG 0.705744 8.3:-22140443 None:intergenic
AGAGACGTGAAACAGCGTCA+CGG 0.722636 8.3:+22140926 MS.gene011717:CDS
GACGTGAAACAGCGTCACGG+TGG 0.727045 8.3:+22140929 MS.gene011717:CDS
ATGCACGTGCGGTTTCAAGG+AGG 0.744530 8.3:-22140021 None:intergenic
TGACTACAGTGGTTAAACGG+AGG 0.748940 8.3:-22140432 None:intergenic
AAGATATAAACAAATCACGG+TGG 0.755243 8.3:-22140666 None:intergenic
GGTGAGGCGTATCATCAGAG+CGG 0.774567 8.3:-22140411 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAATTATGTGAAGTTT+AGG - chr8.3:22140340-22140359 None:intergenic 20.0%
!! TATCAATTATGTGAAGTTTA+GGG - chr8.3:22140339-22140358 None:intergenic 20.0%
! GCAAAGATATAAACAAATCA+CGG - chr8.3:22140672-22140691 None:intergenic 25.0%
! TATTAAAGAAGATGAAGCTT+TGG + chr8.3:22140597-22140616 MS.gene011717:CDS 25.0%
!! CTTCAACAACTTTTATTACA+TGG + chr8.3:22139778-22139797 MS.gene011717:CDS 25.0%
!!! GTTTTAAAGAGTGTTTAAGA+TGG + chr8.3:22140776-22140795 MS.gene011717:CDS 25.0%
!!! TTTAGAAGTTTGTTTTGGTT+CGG + chr8.3:22140741-22140760 MS.gene011717:CDS 25.0%
AACTCTTTACATTCGATATC+CGG + chr8.3:22140634-22140653 MS.gene011717:CDS 30.0%
AAGATATAAACAAATCACGG+TGG - chr8.3:22140669-22140688 None:intergenic 30.0%
ATTCAAAGTTGAAGTAATCG+TGG - chr8.3:22139973-22139992 None:intergenic 30.0%
CAAAAAGTCATGAAGGAAAT+CGG + chr8.3:22140478-22140497 MS.gene011717:CDS 30.0%
GAAAGAGTAATTATCAGTTG+TGG - chr8.3:22139942-22139961 None:intergenic 30.0%
TACTTCAACTTTGAATTCTC+CGG + chr8.3:22139977-22139996 MS.gene011717:CDS 30.0%
TGTTTATATCTTTGCTTCGT+GGG + chr8.3:22140677-22140696 MS.gene011717:CDS 30.0%
TTCAAAGTTGAAGTAATCGT+GGG - chr8.3:22139972-22139991 None:intergenic 30.0%
TTGTTTATATCTTTGCTTCG+TGG + chr8.3:22140676-22140695 MS.gene011717:CDS 30.0%
! AGAGTGTTTAAGATGGTTTA+GGG + chr8.3:22140783-22140802 MS.gene011717:CDS 30.0%
! ATGAAGTTTGTGATGATGTT+AGG + chr8.3:22140998-22141017 MS.gene011717:CDS 30.0%
! TGAAGTTTGTGATGATGTTA+GGG + chr8.3:22140999-22141018 MS.gene011717:CDS 30.0%
! TGTTGAGAAGGTATAAAGAA+GGG + chr8.3:22141025-22141044 MS.gene011717:CDS 30.0%
! TTGATATGTTGTTTGTCTTG+AGG - chr8.3:22139726-22139745 None:intergenic 30.0%
! TTGTTGAGAAGGTATAAAGA+AGG + chr8.3:22141024-22141043 MS.gene011717:CDS 30.0%
!! AAGAGTGTTTAAGATGGTTT+AGG + chr8.3:22140782-22140801 MS.gene011717:CDS 30.0%
!! GCTGATTTAGAAGTTTGTTT+TGG + chr8.3:22140736-22140755 MS.gene011717:CDS 30.0%
!! TAAGTAGTGTTTGTTGTGTT+TGG - chr8.3:22139899-22139918 None:intergenic 30.0%
AGAGTAATTATCAGTTGTGG+TGG - chr8.3:22139939-22139958 None:intergenic 35.0%
AGTAATTATCAGTTGTGGTG+GGG - chr8.3:22139937-22139956 None:intergenic 35.0%
ATTCTTCGTCTTCTTCTTGT+TGG - chr8.3:22139757-22139776 None:intergenic 35.0%
GAGTAATTATCAGTTGTGGT+GGG - chr8.3:22139938-22139957 None:intergenic 35.0%
TCAACAACAGTTAAAACCCT+AGG - chr8.3:22140709-22140728 None:intergenic 35.0%
TTGTGATCCTTATGAGTCAA+TGG + chr8.3:22140903-22140922 MS.gene011717:CDS 35.0%
TTTCAAGAACAAGTAGTGAG+AGG + chr8.3:22140833-22140852 MS.gene011717:CDS 35.0%
! CAAGGATGGAAAAATTTGCT+AGG + chr8.3:22140503-22140522 MS.gene011717:CDS 35.0%
!! ATAGTAGTAGTGGATGCATA+AGG - chr8.3:22139869-22139888 None:intergenic 35.0%
!! GAAAAATTTGCTAGGCTCAT+GGG + chr8.3:22140511-22140530 MS.gene011717:CDS 35.0%
!! GCTATCTTAGAAGCATTAGA+AGG + chr8.3:22140310-22140329 MS.gene011717:CDS 35.0%
!! GTGTTGTTTAGAAGAAGATG+AGG - chr8.3:22139834-22139853 None:intergenic 35.0%
!! TGGAAGAGGTATTGTTGATT+TGG + chr8.3:22140876-22140895 MS.gene011717:CDS 35.0%
!!! GGTTTTAACTGTTGTTGAAG+AGG + chr8.3:22140711-22140730 MS.gene011717:CDS 35.0%
AAACAAATCACGGTGGTTTC+CGG - chr8.3:22140662-22140681 None:intergenic 40.0%
AAAGTTGAAGTAATCGTGGG+AGG - chr8.3:22139969-22139988 None:intergenic 40.0%
AAGTTGAAGTAATCGTGGGA+GGG - chr8.3:22139968-22139987 None:intergenic 40.0%
AGAGCTGCTAGAAGTTGTTA+AGG - chr8.3:22139699-22139718 None:intergenic 40.0%
AGTTCCAAGAAGTTAGTCCA+TGG + chr8.3:22140257-22140276 MS.gene011717:CDS 40.0%
CAAAACATTAACATCCGCGT+CGG + chr8.3:22140192-22140211 MS.gene011717:CDS 40.0%
CTCAGTGCAAAAAGTCATGA+AGG + chr8.3:22140471-22140490 MS.gene011717:CDS 40.0%
CTTCCACGACAAGAATATTC+CGG - chr8.3:22141085-22141104 None:intergenic 40.0%
GCAACACTTATTGCACTCAA+TGG + chr8.3:22140362-22140381 MS.gene011717:CDS 40.0%
GTAATTATCAGTTGTGGTGG+GGG - chr8.3:22139936-22139955 None:intergenic 40.0%
TCCACATGAGTTGTTGGATT+CGG - chr8.3:22140066-22140085 None:intergenic 40.0%
TGGAGTAGTGATAGTAGTAG+TGG - chr8.3:22139879-22139898 None:intergenic 40.0%
TTAGCATCCACATGAGTTGT+TGG - chr8.3:22140072-22140091 None:intergenic 40.0%
TTGATGCTAGAAAGAGAAGC+TGG + chr8.3:22140856-22140875 MS.gene011717:CDS 40.0%
TTTGATCTGTGTCACCGTAT+GGG - chr8.3:22140108-22140127 None:intergenic 40.0%
! CGCGGATGTTAATGTTTTGT+AGG - chr8.3:22140191-22140210 None:intergenic 40.0%
! GATTCGGTTTGTGTTGTTGT+CGG - chr8.3:22140050-22140069 None:intergenic 40.0%
! TCATGAAGGAAATCGGTTCA+AGG + chr8.3:22140485-22140504 MS.gene011717:CDS 40.0%
! TGAGAAGGTATAAAGAAGGG+TGG + chr8.3:22141028-22141047 MS.gene011717:CDS 40.0%
! TTGTCCATGGACTAACTTCT+TGG - chr8.3:22140264-22140283 None:intergenic 40.0%
! TTTTGATCTGTGTCACCGTA+TGG - chr8.3:22140109-22140128 None:intergenic 40.0%
!! ATGTTAGGGCTTTGTTGAGA+AGG + chr8.3:22141013-22141032 MS.gene011717:CDS 40.0%
!! GGAAAAATTTGCTAGGCTCA+TGG + chr8.3:22140510-22140529 MS.gene011717:CDS 40.0%
!!! TTGTTTTGGTTCGGATTTCG+TGG + chr8.3:22140750-22140769 MS.gene011717:CDS 40.0%
AAAATGCACGTGCGGTTTCA+AGG - chr8.3:22140027-22140046 None:intergenic 45.0%
AACGAGCTTAGTTCCCCATA+CGG + chr8.3:22140091-22140110 MS.gene011717:CDS 45.0%
ACCGAATCCAACAACTCATG+TGG + chr8.3:22140062-22140081 MS.gene011717:CDS 45.0%
ATGCCGGAATATTCTTGTCG+TGG + chr8.3:22141079-22141098 MS.gene011717:CDS 45.0%
CATCTTCCTCGTTGAATCGA+AGG - chr8.3:22140230-22140249 None:intergenic 45.0%
CATGTTCCTTCGATTCAACG+AGG + chr8.3:22140221-22140240 MS.gene011717:CDS 45.0%
CGGAATATTCTTGTCGTGGA+AGG + chr8.3:22141083-22141102 MS.gene011717:CDS 45.0%
CGGTGACTACAGTGGTTAAA+CGG - chr8.3:22140438-22140457 None:intergenic 45.0%
CTAGAAAGAGAAGCTGGAAG+AGG + chr8.3:22140862-22140881 MS.gene011717:CDS 45.0%
GAATATTCCGGCATCACCAT+CGG - chr8.3:22141073-22141092 None:intergenic 45.0%
GCTAGAAGTTGTTAAGGTGG+TGG - chr8.3:22139693-22139712 None:intergenic 45.0%
GCTGCTAGAAGTTGTTAAGG+TGG - chr8.3:22139696-22139715 None:intergenic 45.0%
GTTAGTCCATGGACAACGTT+TGG + chr8.3:22140268-22140287 MS.gene011717:CDS 45.0%
TGACTACAGTGGTTAAACGG+AGG - chr8.3:22140435-22140454 None:intergenic 45.0%
TTACATTCGATATCCGGAGC+CGG + chr8.3:22140640-22140659 MS.gene011717:CDS 45.0%
TTGAATTCTCCGGCAACTCA+TGG + chr8.3:22139987-22140006 MS.gene011717:CDS 45.0%
TTGATCTGTGTCACCGTATG+GGG - chr8.3:22140107-22140126 None:intergenic 45.0%
! AGAAGTTGTTAAGGTGGTGG+TGG - chr8.3:22139690-22139709 None:intergenic 45.0%
! GAAGGAAATCGGTTCAAGGA+TGG + chr8.3:22140489-22140508 MS.gene011717:CDS 45.0%
! GTTTTGTGACCATGAGTTGC+CGG - chr8.3:22139999-22140018 None:intergenic 45.0%
!! CGTCTCTCCATTGACTCATA+AGG - chr8.3:22140913-22140932 None:intergenic 45.0%
!! GCATGGTGGTGGGTTTAATA+CGG + chr8.3:22140963-22140982 MS.gene011717:CDS 45.0%
!! TGCTTCTAAGATAGCACCGT+TGG - chr8.3:22140305-22140324 None:intergenic 45.0%
!! TTTGGTTCGGATTTCGTGGA+AGG + chr8.3:22140754-22140773 MS.gene011717:CDS 45.0%
!!! GGAACATGTTTTTTCCGACG+CGG - chr8.3:22140209-22140228 None:intergenic 45.0%
!!! AAAATAATTTTGAATTTAAA+CGG - chr8.3:22140541-22140560 None:intergenic 5.0%
ACAGTGGTTAAACGGAGGTG+AGG - chr8.3:22140430-22140449 None:intergenic 50.0%
ACGTGTCCAAACGTTGTCCA+TGG - chr8.3:22140277-22140296 None:intergenic 50.0%
AGAGACGTGAAACAGCGTCA+CGG + chr8.3:22140926-22140945 MS.gene011717:CDS 50.0%
ATGACACAGTGTTCCTCCGA+TGG + chr8.3:22141054-22141073 MS.gene011717:CDS 50.0%
ATGGACAACGTTTGGACACG+TGG + chr8.3:22140276-22140295 MS.gene011717:CDS 50.0%
CTTGTCGTGGAAGGATAAGC+CGG + chr8.3:22141092-22141111 MS.gene011717:CDS 50.0%
GTGGAGTTGAAGGAAGGATC+AGG - chr8.3:22139671-22139690 None:intergenic 50.0%
GTTGTCGGAAAATGCACGTG+CGG - chr8.3:22140035-22140054 None:intergenic 50.0%
GTTTAGTCGCATGAACGACG+CGG + chr8.3:22140156-22140175 MS.gene011717:CDS 50.0%
TTTAGTCGCATGAACGACGC+GGG + chr8.3:22140157-22140176 MS.gene011717:CDS 50.0%
!! GCTTCGTGGGTTAGAACCTA+GGG + chr8.3:22140690-22140709 MS.gene011717:CDS 50.0%
!! TAAGGTGGTGGTGGAGTTGA+AGG - chr8.3:22139681-22139700 None:intergenic 50.0%
!! TGCTTCGTGGGTTAGAACCT+AGG + chr8.3:22140689-22140708 MS.gene011717:CDS 50.0%
ACTGTAGTCACCGCAGTCAG+CGG + chr8.3:22140445-22140464 MS.gene011717:CDS 55.0%
ATGCACGTGCGGTTTCAAGG+AGG - chr8.3:22140024-22140043 None:intergenic 55.0%
GAAGGATAAGCCGGTGGTGT+GGG + chr8.3:22141101-22141120 MS.gene011717:CDS 55.0%
GGTGAGGCGTATCATCAGAG+CGG - chr8.3:22140414-22140433 None:intergenic 55.0%
GTGAGGCGTATCATCAGAGC+GGG - chr8.3:22140413-22140432 None:intergenic 55.0%
TATTCCGGCATCACCATCGG+AGG - chr8.3:22141070-22141089 None:intergenic 55.0%
TTTGGACACGTGGCAGCCAA+CGG + chr8.3:22140286-22140305 MS.gene011717:CDS 55.0%
! TGTTCCTCCGATGGTGATGC+CGG + chr8.3:22141063-22141082 MS.gene011717:CDS 55.0%
!! GTGGTGGTGGAGTTGAAGGA+AGG - chr8.3:22139677-22139696 None:intergenic 55.0%
AAACAGCGTCACGGTGGAGG+AGG + chr8.3:22140935-22140954 MS.gene011717:CDS 60.0%
AGGAGGAGGTTGCATGGTGG+TGG + chr8.3:22140952-22140971 MS.gene011717:CDS 60.0%
AGGCGTATCATCAGAGCGGG+TGG - chr8.3:22140410-22140429 None:intergenic 60.0%
ATCACGGTGGTTTCCGGCTC+CGG - chr8.3:22140656-22140675 None:intergenic 60.0%
CATACACTGGCCCACACCAC+CGG - chr8.3:22141114-22141133 None:intergenic 60.0%
GACGTGAAACAGCGTCACGG+TGG + chr8.3:22140929-22140948 MS.gene011717:CDS 60.0%
GCCAACACTCCTCGAAGCGT+TGG + chr8.3:22140384-22140403 MS.gene011717:CDS 60.0%
GCTGACTGCGGTGACTACAG+TGG - chr8.3:22140446-22140465 None:intergenic 60.0%
GGAAGGATAAGCCGGTGGTG+TGG + chr8.3:22141100-22141119 MS.gene011717:CDS 60.0%
GGAGGAGGTTGCATGGTGGT+GGG + chr8.3:22140953-22140972 MS.gene011717:CDS 60.0%
GTCGTGGAAGGATAAGCCGG+TGG + chr8.3:22141095-22141114 MS.gene011717:CDS 60.0%
GTGAAACAGCGTCACGGTGG+AGG + chr8.3:22140932-22140951 MS.gene011717:CDS 60.0%
TGGAGGAGGAGGTTGCATGG+TGG + chr8.3:22140949-22140968 MS.gene011717:CDS 60.0%
TGGTGTGGGCCAGTGTATGG+AGG + chr8.3:22141115-22141134 MS.gene011717:CDS 60.0%
! GCCAACGCTTCGAGGAGTGT+TGG - chr8.3:22140388-22140407 None:intergenic 60.0%
CGGTGGAGGAGGAGGTTGCA+TGG + chr8.3:22140946-22140965 MS.gene011717:CDS 65.0%
CGGTGGTGTGGGCCAGTGTA+TGG + chr8.3:22141112-22141131 MS.gene011717:CDS 65.0%
GTAGTCACCGCAGTCAGCGG+TGG + chr8.3:22140448-22140467 MS.gene011717:CDS 65.0%
! CACTGAGCCACCGCTGACTG+CGG - chr8.3:22140458-22140477 None:intergenic 65.0%
AGCGGGTGGCCAACGCTTCG+AGG - chr8.3:22140396-22140415 None:intergenic 70.0%
CAGCGTCACGGTGGAGGAGG+AGG + chr8.3:22140938-22140957 MS.gene011717:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 22139611 22141143 22139611 ID=MS.gene011717
chr8.3 mRNA 22139611 22141143 22139611 ID=MS.gene011717.t1;Parent=MS.gene011717
chr8.3 exon 22139611 22141143 22139611 ID=MS.gene011717.t1.exon1;Parent=MS.gene011717.t1
chr8.3 CDS 22139611 22141143 22139611 ID=cds.MS.gene011717.t1;Parent=MS.gene011717.t1
Gene Sequence

>MS.gene011717

ATGGATACGTTGTTTAGGCTTGTCAATTTTCAACAACAACAACAACAACAATATCAACCTGATCCTTCCTTCAACTCCACCACCACCTTAACAACTTCTAGCAGCTCTAGATCCTCAAGACAAACAACATATCAATATTACAACCAACAAGAAGAAGACGAAGAATGCTTCAACAACTTTTATTACATGGATCATAATAACAACAATGATGAAGATTTATCCTCATCTTCTTCTAAACAACACTATTATACTTATCCTTATGCATCCACTACTACTATCACTACTCCAAACACAACAAACACTACTTATAATACTATCAACACCCCCACCACAACTGATAATTACTCTTTCTCACCCTCCCACGATTACTTCAACTTTGAATTCTCCGGCAACTCATGGTCACAAAACATCCTCCTTGAAACCGCACGTGCATTTTCCGACAACAACACAAACCGAATCCAACAACTCATGTGGATGCTAAACGAGCTTAGTTCCCCATACGGTGACACAGATCAAAAACTTTCATCTTATTTTCTTCAAGCTTTGTTTAGTCGCATGAACGACGCGGGAGATAGAACCTACAAAACATTAACATCCGCGTCGGAAAAAACATGTTCCTTCGATTCAACGAGGAAGATGTTGTTGAAGTTCCAAGAAGTTAGTCCATGGACAACGTTTGGACACGTGGCAGCCAACGGTGCTATCTTAGAAGCATTAGAAGGTAACCCTAAACTTCACATAATTGATATTAGCAACACTTATTGCACTCAATGGCCAACACTCCTCGAAGCGTTGGCCACCCGCTCTGATGATACGCCTCACCTCCGTTTAACCACTGTAGTCACCGCAGTCAGCGGTGGCTCAGTGCAAAAAGTCATGAAGGAAATCGGTTCAAGGATGGAAAAATTTGCTAGGCTCATGGGAGTGCCGTTTAAATTCAAAATTATTTTCAGTGATTTAAGAGAGTTGAATTTGTGTGATTTAGATATTAAAGAAGATGAAGCTTTGGCTATAAATTGTGTAAACTCTTTACATTCGATATCCGGAGCCGGAAACCACCGTGATTTGTTTATATCTTTGCTTCGTGGGTTAGAACCTAGGGTTTTAACTGTTGTTGAAGAGGAAGCTGATTTAGAAGTTTGTTTTGGTTCGGATTTCGTGGAAGGTTTTAAAGAGTGTTTAAGATGGTTTAGGGTTTATTTTGAAGCACTTGATGAAAGTTTTTCAAGAACAAGTAGTGAGAGGTTGATGCTAGAAAGAGAAGCTGGAAGAGGTATTGTTGATTTGGTTGCTTGTGATCCTTATGAGTCAATGGAGAGACGTGAAACAGCGTCACGGTGGAGGAGGAGGTTGCATGGTGGTGGGTTTAATACGGTGTCGTTTAGCGATGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGAAGGTATAAAGAAGGGTGGTCGATGACACAGTGTTCCTCCGATGGTGATGCCGGAATATTCTTGTCGTGGAAGGATAAGCCGGTGGTGTGGGCCAGTGTATGGAGGCCTTGA
Protein sequence

>MS.gene011717.t1

MDTLFRLVNFQQQQQQQYQPDPSFNSTTTLTTSSSSRSSRQTTYQYYNQQEEDEECFNNFYYMDHNNNNDEDLSSSSSKQHYYTYPYASTTTITTPNTTNTTYNTINTPTTTDNYSFSPSHDYFNFEFSGNSWSQNILLETARAFSDNNTNRIQQLMWMLNELSSPYGDTDQKLSSYFLQALFSRMNDAGDRTYKTLTSASEKTCSFDSTRKMLLKFQEVSPWTTFGHVAANGAILEALEGNPKLHIIDISNTYCTQWPTLLEALATRSDDTPHLRLTTVVTAVSGGSVQKVMKEIGSRMEKFARLMGVPFKFKIIFSDLRELNLCDLDIKEDEALAINCVNSLHSISGAGNHRDLFISLLRGLEPRVLTVVEEEADLEVCFGSDFVEGFKECLRWFRVYFEALDESFSRTSSERLMLEREAGRGIVDLVACDPYESMERRETASRWRRRLHGGGFNTVSFSDEVCDDVRALLRRYKEGWSMTQCSSDGDAGIFLSWKDKPVVWASVWRP