Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011725.t1 | XP_013457495.1 | 91.3 | 69 | 6 | 0 | 1 | 69 | 60 | 128 | 4.90E-27 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011725.t1 | Q42381 | 62.5 | 72 | 19 | 2 | 1 | 69 | 55 | 121 | 4.1e-14 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011725.t1 | A0A072UQ97 | 91.3 | 69 | 6 | 0 | 1 | 69 | 60 | 128 | 3.5e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene011725.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011725.t1 | MTR_4g095700 | 91.304 | 69 | 6 | 0 | 1 | 69 | 60 | 128 | 4.92e-38 | 130 |
| MS.gene011725.t1 | MTR_5g015810 | 69.565 | 69 | 15 | 2 | 1 | 69 | 62 | 124 | 1.64e-25 | 96.7 |
| MS.gene011725.t1 | MTR_5g015810 | 69.492 | 59 | 12 | 2 | 11 | 69 | 1 | 53 | 4.14e-21 | 84.0 |
| MS.gene011725.t1 | MTR_4g007130 | 53.968 | 63 | 10 | 2 | 7 | 69 | 84 | 127 | 1.47e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011725.t1 | AT4G37580 | 59.459 | 74 | 18 | 3 | 1 | 69 | 55 | 121 | 1.78e-19 | 80.1 |
| MS.gene011725.t1 | AT2G23060 | 58.442 | 77 | 21 | 3 | 1 | 69 | 58 | 131 | 5.28e-19 | 79.0 |
| MS.gene011725.t1 | AT4G37580 | 61.538 | 65 | 16 | 2 | 7 | 69 | 7 | 64 | 8.90e-19 | 77.8 |
| MS.gene011725.t1 | AT5G67430 | 59.420 | 69 | 21 | 2 | 1 | 69 | 56 | 117 | 1.59e-15 | 69.3 |
Find 0 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTCTTAAGCCTAAAACAT+AGG | + | 32616:15455-15474 | MS.gene011724:intergenic | 30.0% | |
| AATCATAGAAGAATGGGAAT+AGG | - | 32616:15481-15500 | MS.gene011725:CDS | 30.0% | |
| ATTCCCATTCTTCTATGATT+TGG | + | 32616:15481-15500 | MS.gene011725:intergenic | 30.0% | |
| TCTCCAAATCATAGAAGAAT+GGG | - | 32616:15475-15494 | MS.gene011725:CDS | 30.0% | |
| TTCTCCAAATCATAGAAGAA+TGG | - | 32616:15474-15493 | MS.gene011725:CDS | 30.0% | |
| !! | ACTAAACTAGCCTATGTTTT+AGG | - | 32616:15442-15461 | MS.gene011725:CDS | 30.0% |
| AATGGGAATAGGACTAAAAC+TGG | - | 32616:15492-15511 | MS.gene011725:CDS | 35.0% | |
| TGTATCAAAACCGTTACATG+CGG | - | 32616:15349-15368 | MS.gene011725:CDS | 35.0% | |
| !! | AGGCTAGTTTAGTGAAAACA+GGG | + | 32616:15435-15454 | MS.gene011725:intergenic | 35.0% |
| !! | TAGGCTAGTTTAGTGAAAAC+AGG | + | 32616:15436-15455 | MS.gene011725:intergenic | 35.0% |
| !!! | TCTGTGATGGTGTTTTTTGT+TGG | + | 32616:15391-15410 | MS.gene011725:intergenic | 35.0% |
| ACAGGGACATGATTGGAATT+TGG | + | 32616:15418-15437 | MS.gene011725:intergenic | 40.0% | |
| AGTGAAAACAGGGACATGAT+TGG | + | 32616:15425-15444 | MS.gene011725:intergenic | 40.0% | |
| !! | GGAATTTGGTGTTTCTGTGA+TGG | + | 32616:15404-15423 | MS.gene011725:intergenic | 40.0% |
| !!! | GAGTTTTTTGCCGCATGTAA+CGG | + | 32616:15362-15381 | MS.gene011725:intergenic | 40.0% |
| GAGATAGGCAATGAGACAGT+CGG | - | 32616:15313-15332 | MS.gene011725:CDS | 45.0% | |
| GAGACAGTCGGAATGATACG+AGG | - | 32616:15325-15344 | MS.gene011725:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32616 | gene | 15307 | 15513 | 15307 | ID=MS.gene011725 |
| 32616 | mRNA | 15307 | 15513 | 15307 | ID=MS.gene011725.t1;Parent=MS.gene011725 |
| 32616 | exon | 15307 | 15513 | 15307 | ID=MS.gene011725.t1.exon1;Parent=MS.gene011725.t1 |
| 32616 | CDS | 15307 | 15513 | 15307 | ID=cds.MS.gene011725.t1;Parent=MS.gene011725.t1 |
| Gene Sequence |
| Protein sequence |
