AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011735.t1 MTR_4g095500 98.652 445 4 1 1 443 25 469 0.0 913
MS.gene011735.t1 MTR_5g015950 68.502 454 125 8 1 443 1 447 0.0 616
MS.gene011735.t1 MTR_3g053270 68.502 454 125 8 1 443 1 447 0.0 616
MS.gene011735.t1 MTR_2g089100 43.488 430 199 5 28 443 45 444 1.64e-121 362
MS.gene011735.t1 MTR_4g097080 29.356 419 243 10 33 443 131 504 1.15e-54 190
MS.gene011735.t1 MTR_5g015490 29.469 414 251 8 35 443 114 491 4.38e-53 185
MS.gene011735.t1 MTR_2g099110 29.508 427 237 12 34 443 73 452 1.28e-48 172
MS.gene011735.t1 MTR_3g089055 27.358 424 264 7 19 441 189 569 5.91e-43 159
MS.gene011735.t1 MTR_3g089055 27.358 424 264 7 19 441 184 564 7.96e-43 159
MS.gene011735.t1 MTR_1g029420 27.073 410 257 7 33 441 223 591 8.67e-42 156
MS.gene011735.t1 MTR_5g097480 27.163 416 251 9 32 441 174 543 5.60e-39 148
MS.gene011735.t1 MTR_4g133660 27.381 420 248 10 32 442 172 543 1.81e-38 146
MS.gene011735.t1 MTR_8g442410 27.119 413 250 12 36 441 156 524 6.54e-37 142
MS.gene011735.t1 MTR_5g094450 25.728 412 266 8 32 441 158 531 2.49e-36 140
MS.gene011735.t1 MTR_3g056110 24.703 421 270 9 23 441 166 541 9.86e-36 139
MS.gene011735.t1 MTR_8g020840 28.276 435 240 13 28 441 164 547 5.01e-35 137
MS.gene011735.t1 MTR_2g082090 25.240 416 259 7 32 441 209 578 6.69e-35 137
MS.gene011735.t1 MTR_7g027190 26.316 418 252 9 35 441 300 672 1.25e-33 134
MS.gene011735.t1 MTR_2g026250 25.728 412 263 9 32 441 225 595 3.47e-31 126
MS.gene011735.t1 MTR_3g065980 25.296 423 243 10 36 441 173 539 1.61e-30 124
MS.gene011735.t1 MTR_4g064150 23.831 449 290 11 3 444 332 735 1.81e-30 125
MS.gene011735.t1 MTR_3g022830 24.091 440 270 10 36 441 26 435 2.36e-30 122
MS.gene011735.t1 MTR_2g097410 23.741 417 273 10 32 444 368 743 6.76e-28 117
MS.gene011735.t1 MTR_4g064180 24.163 418 267 13 32 442 251 625 8.95e-28 116
MS.gene011735.t1 MTR_4g104020 26.168 428 240 14 34 441 148 519 1.56e-27 115
MS.gene011735.t1 MTR_4g076140 24.124 485 286 13 5 441 18 468 4.02e-27 113
MS.gene011735.t1 MTR_4g064120 23.981 417 269 12 32 442 251 625 6.59e-27 114
MS.gene011735.t1 MTR_4g064160 24.524 420 264 12 32 444 313 686 6.93e-27 114
MS.gene011735.t1 MTR_5g009080 23.881 469 286 10 15 441 38 477 6.94e-27 112
MS.gene011735.t1 MTR_4g064200 25.542 415 256 12 35 443 284 651 1.02e-26 113
MS.gene011735.t1 MTR_7g062120 23.684 418 270 9 32 444 369 742 1.28e-26 113
MS.gene011735.t1 MTR_2g097310 23.684 418 268 10 32 444 269 640 1.35e-24 107
MS.gene011735.t1 MTR_6g047750 26.190 420 252 10 31 441 291 661 1.47e-24 107
MS.gene011735.t1 MTR_2g097390 23.326 433 279 11 17 443 257 642 3.72e-23 102
MS.gene011735.t1 MTR_2g097467 22.276 413 273 8 35 444 289 656 1.05e-22 101
MS.gene011735.t1 MTR_2g097350 22.512 422 268 10 32 444 271 642 4.30e-22 99.8
MS.gene011735.t1 MTR_4g102790 21.770 418 274 11 35 444 292 664 1.85e-21 97.8
MS.gene011735.t1 MTR_4g077760 25.882 425 244 16 30 441 188 554 2.54e-21 97.1
MS.gene011735.t1 MTR_2g097473 21.498 414 275 8 35 444 289 656 3.88e-21 96.7
MS.gene011735.t1 MTR_1g069725 24.700 417 241 16 35 441 109 462 9.10e-21 94.7
MS.gene011735.t1 MTR_2g097463 22.464 414 271 9 35 444 290 657 9.96e-21 95.5
MS.gene011735.t1 MTR_7g057230 24.451 319 199 5 128 441 375 656 1.34e-19 92.0
MS.gene011735.t1 MTR_7g074650 23.301 412 251 14 38 443 441 793 7.78e-19 89.7
MS.gene011735.t1 MTR_2g097380 22.660 406 236 10 42 443 231 562 3.98e-18 87.0
MS.gene011735.t1 MTR_1g096030 24.146 439 241 17 35 441 80 458 1.50e-17 85.1
MS.gene011735.t1 MTR_4g076020 22.439 410 248 14 38 443 87 430 4.00e-16 80.5
MS.gene011735.t1 MTR_5g058860 24.941 421 261 16 34 441 118 496 9.82e-16 79.7
MS.gene011735.t1 MTR_8g093070 22.912 419 253 14 35 443 139 497 1.95e-15 78.6
MS.gene011735.t1 MTR_3g072710 23.515 421 269 14 34 441 119 499 2.00e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011735.t1 AT3G49950 56.591 440 146 10 12 444 9 410 2.34e-171 487
MS.gene011735.t1 AT5G67411 55.947 227 73 4 159 383 1 202 1.03e-76 238
MS.gene011735.t1 AT4G37650 29.621 422 251 8 33 443 144 530 5.67e-52 183
MS.gene011735.t1 AT1G21450 25.547 411 263 7 33 441 223 592 5.09e-39 149
MS.gene011735.t1 AT2G04890 26.034 411 255 8 32 441 50 412 2.29e-38 144
MS.gene011735.t1 AT1G50600 25.176 425 269 10 20 441 147 525 1.56e-37 144
MS.gene011735.t1 AT5G48150 26.988 415 246 10 36 441 123 489 2.40e-37 142
MS.gene011735.t1 AT5G48150 26.988 415 246 10 36 441 123 489 2.40e-37 142
MS.gene011735.t1 AT5G48150 26.988 415 246 10 36 441 123 489 2.40e-37 142
MS.gene011735.t1 AT5G48150 26.988 415 246 10 36 441 123 489 2.40e-37 142
MS.gene011735.t1 AT1G50600 25.176 425 269 10 20 441 222 600 2.92e-37 144
MS.gene011735.t1 AT1G50600 25.176 425 269 10 20 441 218 596 3.18e-37 144
MS.gene011735.t1 AT4G17230 26.988 415 257 8 32 443 155 526 5.21e-34 134
MS.gene011735.t1 AT4G17230 26.988 415 257 8 32 443 155 526 5.21e-34 134
MS.gene011735.t1 AT1G50420 25.835 449 264 11 35 441 57 478 1.71e-31 126
MS.gene011735.t1 AT3G03450 25.362 414 248 9 36 441 184 544 1.85e-30 124
MS.gene011735.t1 AT5G17490 24.818 411 252 8 34 441 159 515 2.92e-30 123
MS.gene011735.t1 AT2G29065 26.316 418 261 11 35 444 252 630 8.50e-30 122
MS.gene011735.t1 AT3G46600 25.060 419 260 9 32 443 1 372 1.10e-28 116
MS.gene011735.t1 AT3G46600 25.000 420 261 9 32 444 209 581 7.68e-28 116
MS.gene011735.t1 AT3G46600 25.060 419 260 9 32 443 177 548 8.17e-28 116
MS.gene011735.t1 AT5G52510 23.040 421 269 7 31 441 264 639 1.10e-27 116
MS.gene011735.t1 AT2G29060 24.476 429 264 12 28 444 277 657 3.22e-26 112
MS.gene011735.t1 AT2G29060 24.476 429 264 12 28 444 314 694 3.52e-26 112
MS.gene011735.t1 AT1G07530 23.543 429 282 10 19 443 380 766 5.34e-26 111
MS.gene011735.t1 AT2G01570 23.902 410 254 11 36 441 225 580 5.50e-26 111
MS.gene011735.t1 AT3G13840 26.429 420 242 14 33 441 146 509 1.13e-25 109
MS.gene011735.t1 AT5G59450 23.490 447 290 10 6 444 198 600 4.96e-25 108
MS.gene011735.t1 AT1G14920 23.659 410 255 11 36 441 173 528 3.80e-24 105
MS.gene011735.t1 AT2G37650 23.077 416 271 11 32 443 332 702 1.15e-23 104
MS.gene011735.t1 AT2G37650 23.077 416 271 11 32 443 344 714 1.22e-23 104
MS.gene011735.t1 AT5G41920 25.542 415 243 15 35 441 43 399 5.42e-21 95.1
MS.gene011735.t1 AT1G55580 22.273 440 276 11 32 441 41 444 5.34e-20 92.4
MS.gene011735.t1 AT3G54220 23.971 413 250 15 38 443 296 651 1.07e-19 92.4
MS.gene011735.t1 AT1G63100 22.642 424 264 13 35 441 277 653 4.26e-19 90.5
MS.gene011735.t1 AT5G66770 24.346 382 233 12 34 410 219 549 4.96e-19 90.1
MS.gene011735.t1 AT1G63100 22.642 424 264 13 35 441 252 628 6.22e-19 89.7
MS.gene011735.t1 AT4G08250 25.175 429 242 20 34 441 106 476 2.56e-13 72.0
MS.gene011735.t1 AT3G50650 23.136 389 237 12 64 441 204 541 3.44e-11 65.5

Find 0 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGAGTTTGAATTTGATA+GGG - 32652:48241-48260 None:intergenic 20.0%
! CATATGAGTTTGAATTTGAT+AGG - 32652:48242-48261 None:intergenic 25.0%
! TCTACTAAAATAACAAGTGT+TGG - 32652:48357-48376 None:intergenic 25.0%
! TTCTTTGATTGAACATTTGA+GGG + 32652:48140-48159 MS.gene011735:CDS 25.0%
!! ATGAGAAATGCTAATTTTCA+AGG + 32652:48600-48619 MS.gene011735:CDS 25.0%
!! TGAGAAATGCTAATTTTCAA+GGG + 32652:48601-48620 MS.gene011735:CDS 25.0%
!! TGATTTAACTTCAAACAACT+TGG + 32652:48386-48405 MS.gene011735:CDS 25.0%
AATTTGATAGGGTTTCATCT+GGG - 32652:48230-48249 None:intergenic 30.0%
ACAACTTGGTTAGTAGATTA+AGG + 32652:48400-48419 MS.gene011735:CDS 30.0%
CTTCTTTGATTGAACATTTG+AGG + 32652:48139-48158 MS.gene011735:CDS 30.0%
GGTCTGCATTTAATTATCTA+TGG + 32652:48421-48440 MS.gene011735:CDS 30.0%
TAAGAAAGATCAAGTTTAGG+TGG - 32652:48024-48043 None:intergenic 30.0%
TCGTAAGAAAGATCAAGTTT+AGG - 32652:48027-48046 None:intergenic 30.0%
! GTTTCTTCAAGTTACACAAA+TGG + 32652:48111-48130 MS.gene011735:CDS 30.0%
! TTGTTCTACTAGCATTTTAC+AGG - 32652:47707-47726 None:intergenic 30.0%
!! AATGCTAATTTTCAAGGGAT+TGG + 32652:48606-48625 MS.gene011735:CDS 30.0%
!! AATTTTCAAGGGATTGGATT+TGG + 32652:48612-48631 MS.gene011735:CDS 30.0%
!! TAGTTAAAGCTTTGGATGTT+GGG - 32652:47492-47511 None:intergenic 30.0%
AATGTGTCCACAGTATCATA+AGG - 32652:48450-48469 None:intergenic 35.0%
ACCAAAGGTAGTTAAAGCTT+TGG - 32652:47500-47519 None:intergenic 35.0%
ACTTGCTCAACAAATCCTTT+GGG + 32652:47585-47604 MS.gene011735:CDS 35.0%
AGTTGAACCTATAGGCAAAT+GGG + 32652:48569-48588 MS.gene011735:CDS 35.0%
ATGAGCATATTGTGCTTACT+TGG + 32652:48706-48725 MS.gene011735:CDS 35.0%
ATGGATACCTTATGATACTG+TGG + 32652:48440-48459 MS.gene011735:CDS 35.0%
CATATTGTGCTTACTTGGAA+AGG + 32652:48711-48730 MS.gene011735:CDS 35.0%
GAAACAGTGAGTTTGATCAA+AGG - 32652:47967-47986 None:intergenic 35.0%
GAATTTGATAGGGTTTCATC+TGG - 32652:48231-48250 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + 32652:48033-48052 MS.gene011735:CDS 35.0%
GGTATGAAGCAGATATTTGT+TGG + 32652:48499-48518 MS.gene011735:CDS 35.0%
TGAGAATCTGTGTGTATCTA+TGG - 32652:47758-47777 None:intergenic 35.0%
! GTTCAACGTTTGGTTTATTC+AGG + 32652:48162-48181 MS.gene011735:CDS 35.0%
! TACTAGCATTTTACAGGTAC+CGG - 32652:47701-47720 None:intergenic 35.0%
! TCCAAAGCTTTAACTACCTT+TGG + 32652:47496-47515 MS.gene011735:CDS 35.0%
!! GTAGTTAAAGCTTTGGATGT+TGG - 32652:47493-47512 None:intergenic 35.0%
!!! ATGTTGTTTTTGCTTCTGCT+TGG + 32652:48739-48758 MS.gene011735:CDS 35.0%
AACAACATAGCACCACAAGA+TGG + 32652:47613-47632 MS.gene011735:CDS 40.0%
ACATTTGAGGGTTCAACGTT+TGG + 32652:48152-48171 MS.gene011735:CDS 40.0%
ACTTCGTGGATTTAACACCT+TGG + 32652:47794-47813 MS.gene011735:CDS 40.0%
AGTAGTTGCAGTAGTAGTAG+AGG - 32652:48280-48299 None:intergenic 40.0%
CACTTGCTCAACAAATCCTT+TGG + 32652:47584-47603 MS.gene011735:CDS 40.0%
CTATGTTGTTGAGAACCCAA+AGG - 32652:47603-47622 None:intergenic 40.0%
CTTTGTTCCCATTTGCCTAT+AGG - 32652:48579-48598 None:intergenic 40.0%
GAGAATGTGATAGCTCATGA+AGG + 32652:48528-48547 MS.gene011735:CDS 40.0%
GAGTTGAACCTATAGGCAAA+TGG + 32652:48568-48587 MS.gene011735:CDS 40.0%
GGTTTATTCAGGTGAAGCTT+TGG + 32652:48173-48192 MS.gene011735:CDS 40.0%
GTGGAAGTAAACAAAGGCAA+TGG + 32652:48478-48497 MS.gene011735:CDS 40.0%
GTTGAGAGAGTTGAACCTAT+AGG + 32652:48561-48580 MS.gene011735:CDS 40.0%
TAGGTCAAGAAACGTGACAT+TGG + 32652:48077-48096 MS.gene011735:CDS 40.0%
TGATTTGAGTCACCATCTTG+TGG - 32652:47628-47647 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + 32652:47805-47824 MS.gene011735:CDS 40.0%
TTGAGCAAGTGTTACATCGT+TGG - 32652:47575-47594 None:intergenic 40.0%
!! CGACGAATTAGGTTCTAAGT+TGG + 32652:48044-48063 MS.gene011735:CDS 40.0%
!! CTAAGTTGGTGAATTTCGCT+AGG + 32652:48058-48077 MS.gene011735:CDS 40.0%
!! GCGATAGCATCGATTAATGT+AGG - 32652:47925-47944 None:intergenic 40.0%
ACAAATCGTGCAGCGAAAAC+CGG + 32652:47679-47698 MS.gene011735:CDS 45.0%
AGCTGCTAATGCAGCAATTC+TGG + 32652:47834-47853 MS.gene011735:CDS 45.0%
ATGCTTGATGAACATGCTGC+TGG + 32652:48663-48682 MS.gene011735:CDS 45.0%
CAGATAAGCCTACCAAACCA+TGG + 32652:47464-47483 MS.gene011735:CDS 45.0%
CATGCAGTTTGTGTCACCAA+AGG - 32652:47515-47534 None:intergenic 45.0%
CATTGAGCTAGCTAACTTCG+TGG + 32652:47780-47799 MS.gene011735:CDS 45.0%
TAACACCTTGGCATCGTTTC+GGG + 32652:47806-47825 MS.gene011735:CDS 45.0%
TGATCAAAGGAGCAACTTCG+TGG - 32652:47954-47973 None:intergenic 45.0%
TGATGAACATGCTGCTGGAT+GGG + 32652:48668-48687 MS.gene011735:CDS 45.0%
TGCCACGTGGAAGTAAACAA+AGG + 32652:48472-48491 MS.gene011735:CDS 45.0%
TTGATGAACATGCTGCTGGA+TGG + 32652:48667-48686 MS.gene011735:CDS 45.0%
TTTCGCTGCACGATTTGTGA+GGG - 32652:47677-47696 None:intergenic 45.0%
! CTTTGGTGACACAAACTGCA+TGG + 32652:47513-47532 MS.gene011735:CDS 45.0%
! GCAATTCTGGAAGCAACAGA+AGG + 32652:47847-47866 MS.gene011735:CDS 45.0%
! TGCCTTTGTTTACTTCCACG+TGG - 32652:48477-48496 None:intergenic 45.0%
! TTGTTTCTTAACGCGCTTCG+AGG + 32652:48324-48343 MS.gene011735:CDS 45.0%
! TTTTCGCTGCACGATTTGTG+AGG - 32652:47678-47697 None:intergenic 45.0%
!! CATGGTTTGGTAGGCTTATC+TGG - 32652:47466-47485 None:intergenic 45.0%
!! GGATTTGGTGAGGAATGTGT+TGG + 32652:48627-48646 MS.gene011735:CDS 45.0%
!! TCAAGGGATTGGATTTGGTG+AGG + 32652:48617-48636 MS.gene011735:CDS 45.0%
CACGATTTGTGAGGGCTCTA+AGG - 32652:47669-47688 None:intergenic 50.0%
GATGAACATGCTGCTGGATG+GGG + 32652:48669-48688 MS.gene011735:CDS 50.0%
GGATGTTGGGAACCATGGTT+TGG - 32652:47479-47498 None:intergenic 50.0%
! GCTTTGGATGTTGGGAACCA+TGG - 32652:47484-47503 None:intergenic 50.0%
! GGTTTGGTAGGCTTATCTGG+TGG - 32652:47463-47482 None:intergenic 50.0%
! TGGTGAGGAATGTGTTGGTG+AGG + 32652:48632-48651 MS.gene011735:CDS 50.0%
!! GTTGGGAACCATGGTTTGGT+AGG - 32652:47475-47494 None:intergenic 50.0%
GTGGACACATTTCTGCCACG+TGG + 32652:48459-48478 MS.gene011735:CDS 55.0%
GCGTACCCGAAACGATGCCA+AGG - 32652:47814-47833 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
32652 gene 47439 48773 47439 ID=MS.gene011735
32652 mRNA 47439 48773 47439 ID=MS.gene011735.t1;Parent=MS.gene011735
32652 exon 47439 48773 47439 ID=MS.gene011735.t1.exon1;Parent=MS.gene011735.t1
32652 CDS 47439 48773 47439 ID=cds.MS.gene011735.t1;Parent=MS.gene011735.t1
Gene Sequence

>MS.gene011735

ATGAACAAGAACAATCAAGTACCACCAGATAAGCCTACCAAACCATGGTTCCCAACATCCAAAGCTTTAACTACCTTTGGTGACACAAACTGCATGGAACAGTTACTTGTTCATTGCGCAAACGCAATCGAAACCAACGATGTAACACTTGCTCAACAAATCCTTTGGGTTCTCAACAACATAGCACCACAAGATGGTGACTCAAATCAACGCTTAGCTTATAGTTTCCTTAGAGCCCTCACAAATCGTGCAGCGAAAACCGGTACCTGTAAAATGCTAGTAGAACAAGTTTATTCAAATGCTCATAACAATCTCACCATAGATACACACAGATTCTCAGTCATTGAGCTAGCTAACTTCGTGGATTTAACACCTTGGCATCGTTTCGGGTACGCAGCTGCTAATGCAGCAATTCTGGAAGCAACAGAAGGTTTCTCAGTGATTCATATAGTTGATTTGAGTTCAACTCATTGCATGCAGATTCCTACATTAATCGATGCTATCGCTAATCGCCACGAAGTTGCTCCTTTGATCAAACTCACTGTTTCTGATCATGCTAACAACAACAGTGATCAACTAATTCCACCTAAACTTGATCTTTCTTACGACGAATTAGGTTCTAAGTTGGTGAATTTCGCTAGGTCAAGAAACGTGACATTGGAATTTAGAGTAGTTTCTTCAAGTTACACAAATGGTTTTGCTTCTTTGATTGAACATTTGAGGGTTCAACGTTTGGTTTATTCAGGTGAAGCTTTGGTTATAAATTGTCACATGATGCTTCATTATATCCCAGATGAAACCCTATCAAATTCAAACTCATATGTTTATGATTCTATGTCCTCTACTACTACTGCAACTACTATTACTACTACGTCTCTTAGATCGTTGTTTCTTAACGCGCTTCGAGGTTTAGATCCAACACTTGTTATTTTAGTAGATGAAGATGTTGATTTAACTTCAAACAACTTGGTTAGTAGATTAAGGTCTGCATTTAATTATCTATGGATACCTTATGATACTGTGGACACATTTCTGCCACGTGGAAGTAAACAAAGGCAATGGTATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGATAGCTCATGAAGGTGTTCAAAGAGTTGAGAGAGTTGAACCTATAGGCAAATGGGAACAAAGAATGAGAAATGCTAATTTTCAAGGGATTGGATTTGGTGAGGAATGTGTTGGTGAGGTAAAAGCTATGCTTGATGAACATGCTGCTGGATGGGGATTGAAGAAAGAAGATGAGCATATTGTGCTTACTTGGAAAGGACATAATGTTGTTTTTGCTTCTGCTTGGTTACCTGTTTAA
Protein sequence

>MS.gene011735.t1

MNKNNQVPPDKPTKPWFPTSKALTTFGDTNCMEQLLVHCANAIETNDVTLAQQILWVLNNIAPQDGDSNQRLAYSFLRALTNRAAKTGTCKMLVEQVYSNAHNNLTIDTHRFSVIELANFVDLTPWHRFGYAAANAAILEATEGFSVIHIVDLSSTHCMQIPTLIDAIANRHEVAPLIKLTVSDHANNNSDQLIPPKLDLSYDELGSKLVNFARSRNVTLEFRVVSSSYTNGFASLIEHLRVQRLVYSGEALVINCHMMLHYIPDETLSNSNSYVYDSMSSTTTATTITTTSLRSLFLNALRGLDPTLVILVDEDVDLTSNNLVSRLRSAFNYLWIPYDTVDTFLPRGSKQRQWYEADICWKIENVIAHEGVQRVERVEPIGKWEQRMRNANFQGIGFGEECVGEVKAMLDEHAAGWGLKKEDEHIVLTWKGHNVVFASAWLPV