AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012055

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012055.t1 MTR_0036s0260 83.824 68 11 0 13 80 28 95 1.50e-36 122
MS.gene012055.t1 MTR_2g090305 80.882 68 13 0 13 80 28 95 3.27e-34 115
MS.gene012055.t1 MTR_4g100630 74.627 67 17 0 14 80 96 162 1.59e-32 114
MS.gene012055.t1 MTR_2g100930 69.014 71 22 0 10 80 106 176 3.20e-32 114
MS.gene012055.t1 MTR_6g092540 73.846 65 17 0 13 77 97 161 1.97e-31 111
MS.gene012055.t1 MTR_5g037080 85.507 69 10 0 13 81 102 170 9.51e-31 109
MS.gene012055.t1 MTR_8g101650 65.789 76 24 2 3 77 94 168 8.96e-30 106
MS.gene012055.t1 MTR_4g111975 68.571 70 22 0 10 79 135 204 6.25e-29 104
MS.gene012055.t1 MTR_4g107230 92.754 69 5 0 13 81 84 152 6.39e-29 104
MS.gene012055.t1 MTR_1g111830 68.182 66 21 0 14 79 125 190 4.70e-28 101
MS.gene012055.t1 MTR_3g462790 68.657 67 21 0 13 79 147 213 1.67e-27 101
MS.gene012055.t1 MTR_5g069710 68.657 67 21 0 13 79 132 198 2.15e-27 100
MS.gene012055.t1 MTR_7g067080 68.182 66 21 0 13 78 140 205 1.15e-26 99.0
MS.gene012055.t1 MTR_5g081860 61.972 71 25 1 13 81 125 195 1.34e-23 90.5
MS.gene012055.t1 MTR_3g104370 57.746 71 27 1 13 80 91 161 1.14e-22 87.0
MS.gene012055.t1 MTR_8g063600 57.746 71 28 1 10 78 103 173 3.86e-22 86.7
MS.gene012055.t1 MTR_4g015130 62.500 56 21 0 23 78 3 58 1.72e-19 75.1
MS.gene012055.t1 MTR_1g048660 54.688 64 29 0 14 77 13 76 6.76e-19 73.9
MS.gene012055.t1 MTR_5g088010 46.053 76 38 1 8 80 55 130 3.86e-18 75.5
MS.gene012055.t1 MTR_1g033620 68.627 51 16 0 29 79 21 71 5.25e-18 75.1
MS.gene012055.t1 MTR_5g027570 46.479 71 36 1 10 78 89 159 1.25e-17 73.9
MS.gene012055.t1 MTR_8g063870 53.030 66 29 1 15 78 94 159 1.64e-17 74.3
MS.gene012055.t1 MTR_5g088060 49.275 69 32 1 15 80 274 342 2.42e-16 72.0
MS.gene012055.t1 MTR_5g488160 49.275 69 32 1 15 80 274 342 2.42e-16 72.0
MS.gene012055.t1 MTR_5g027550 62.500 48 18 0 13 60 77 124 7.30e-16 69.3
MS.gene012055.t1 MTR_1g083180 44.156 77 39 2 8 81 56 131 1.84e-13 62.4
MS.gene012055.t1 MTR_5g075760 46.269 67 33 2 15 79 107 172 1.43e-11 57.8
MS.gene012055.t1 MTR_3g070110 44.444 72 33 1 15 79 97 168 2.96e-11 57.0
MS.gene012055.t1 MTR_5g075790 44.776 67 34 2 15 79 115 180 5.51e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012055.t1 AT5G47390 76.119 67 16 0 14 80 97 163 6.90e-33 116
MS.gene012055.t1 AT1G49010 70.000 70 21 0 10 79 131 200 1.28e-29 107
MS.gene012055.t1 AT5G04760 64.000 75 27 0 5 79 91 165 8.30e-29 102
MS.gene012055.t1 AT1G70000 83.824 68 11 0 14 81 99 166 2.31e-28 102
MS.gene012055.t1 AT1G70000 83.824 68 11 0 14 81 99 166 2.31e-28 102
MS.gene012055.t1 AT2G38090 68.657 67 21 0 13 79 141 207 6.05e-28 102
MS.gene012055.t1 AT5G58900 68.657 67 21 0 13 79 141 207 6.41e-28 102
MS.gene012055.t1 AT5G08520 65.714 70 24 0 10 79 115 184 1.86e-27 100
MS.gene012055.t1 AT5G56840 64.706 68 23 1 13 79 91 158 4.56e-27 98.6
MS.gene012055.t1 AT5G61620 64.179 67 24 0 14 80 109 175 9.59e-27 99.4
MS.gene012055.t1 AT1G74840 84.058 69 11 0 13 81 96 164 5.34e-26 95.9
MS.gene012055.t1 AT1G19000 82.609 69 12 0 13 81 102 170 9.65e-26 96.3
MS.gene012055.t1 AT1G19000 82.609 69 12 0 13 81 102 170 9.65e-26 96.3
MS.gene012055.t1 AT1G74840 84.058 69 11 0 13 81 96 164 1.54e-25 95.5
MS.gene012055.t1 AT3G16350 72.059 68 19 0 13 80 137 204 1.93e-25 97.1
MS.gene012055.t1 AT5G01200 64.179 67 24 0 13 79 146 212 5.60e-25 94.0
MS.gene012055.t1 AT5G05790 60.000 70 28 0 10 79 128 197 7.97e-24 91.3
MS.gene012055.t1 AT5G05790 60.000 70 28 0 10 79 128 197 7.97e-24 91.3
MS.gene012055.t1 AT3G11280 61.194 67 26 0 13 79 127 193 2.48e-23 89.7
MS.gene012055.t1 AT3G11280 61.194 67 26 0 13 79 127 193 2.48e-23 89.7
MS.gene012055.t1 AT4G09450 53.623 69 32 0 10 78 88 156 3.01e-21 82.8
MS.gene012055.t1 AT3G10580 56.061 66 27 1 13 78 95 158 4.51e-21 83.6
MS.gene012055.t1 AT3G10580 56.061 66 27 1 13 78 95 158 4.63e-21 84.0
MS.gene012055.t1 AT5G23650 56.522 69 30 0 10 78 117 185 9.24e-20 81.3
MS.gene012055.t1 AT3G10590 51.471 68 32 1 14 81 112 178 1.03e-17 73.9

Find 21 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAATGTGGCTTACATGATC+GGG 0.431194 8.1:+31044465 None:intergenic
AGAAAGTGGGAAAAGGTGAT+TGG 0.465565 8.1:-31043657 MS.gene012055:CDS
GGGAATGTGGCTTACATGAT+CGG 0.480428 8.1:+31044464 None:intergenic
TTGGTTGGATTGAAGAAAGT+GGG 0.486765 8.1:-31043670 MS.gene012055:CDS
TTTGGTTGGATTGAAGAAAG+TGG 0.534445 8.1:-31043671 MS.gene012055:CDS
GGTGGTGATATCAAAGAGAC+TGG 0.540239 8.1:+31043525 None:intergenic
GGATTGAAGAAAGTGGGAAA+AGG 0.562648 8.1:-31043664 MS.gene012055:CDS
TTGTGCTCTTCCTCAGTCCA+CGG 0.578432 8.1:+31043700 None:intergenic
TATCAAAGAGACTGGATCTA+CGG 0.592657 8.1:+31043533 None:intergenic
GTGATTGGAGAGGAATCTCA+AGG 0.619264 8.1:-31043642 MS.gene012055:CDS
GTGGGAAAAGGTGATTGGAG+AGG 0.624127 8.1:-31043652 MS.gene012055:CDS
TGAATGGATCAGGGATTCCG+TGG 0.626840 8.1:-31043717 MS.gene012055:intron
GGCGATTGAGATTACTTCGG+CGG 0.629813 8.1:+31043560 None:intergenic
TGACATGGAATGGTGTTGCT+CGG 0.634157 8.1:-31044499 None:intergenic
GCATGACTTGCAACATGCGT+AGG 0.635692 8.1:+31043598 None:intergenic
CTTTGATATCACCACCGACA+CGG 0.638033 8.1:-31043518 None:intergenic
GACGGCGATTGAGATTACTT+CGG 0.641222 8.1:+31043557 None:intergenic
ATCAGGGATTCCGTGGACTG+AGG 0.660333 8.1:-31043710 MS.gene012055:intron
GAATGTGGCTTACATGATCG+GGG 0.660918 8.1:+31044466 None:intergenic
AGAGACTGGATCTACGGCGA+CGG 0.684123 8.1:+31043539 None:intergenic
AATGTGGCTTACATGATCGG+GGG 0.708447 8.1:+31044467 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAATATAATAGTTTAA+GGG + chr8.1:31043569-31043588 None:intergenic 10.0%
!! AAAGAATATAATAGTTTAAG+GGG + chr8.1:31043568-31043587 None:intergenic 15.0%
!! CAAAAATAATAAAAATTGGA+TGG + chr8.1:31043734-31043753 None:intergenic 15.0%
!! CTTGCAAAAATAATAAAAAT+TGG + chr8.1:31043738-31043757 None:intergenic 15.0%
!! GAAAAGAATATAATAGTTTA+AGG + chr8.1:31043570-31043589 None:intergenic 15.0%
!! ATTTCAATCAATTCAAACAA+AGG + chr8.1:31044242-31044261 None:intergenic 20.0%
!! TATTGAAGCAAAAATATTCA+TGG + chr8.1:31043678-31043697 None:intergenic 20.0%
!!! TGTTATTGATTTTCTTTGAA+TGG - chr8.1:31044276-31044295 MS.gene012055:intron 20.0%
! AATACAGCACAATTCAAAAT+TGG + chr8.1:31043943-31043962 None:intergenic 25.0%
! AATATCTACTTAAAATCCTG+AGG + chr8.1:31044091-31044110 None:intergenic 25.0%
! CTTCAATATTAATTGTGTCT+TGG - chr8.1:31043690-31043709 MS.gene012055:CDS 25.0%
! TGTGCTGTATTATTCATTTA+GGG - chr8.1:31043952-31043971 MS.gene012055:intron 25.0%
! TTGTGCTGTATTATTCATTT+AGG - chr8.1:31043951-31043970 MS.gene012055:intron 25.0%
!! TCTCACATTTAAATGGTTTT+TGG - chr8.1:31044147-31044166 MS.gene012055:intron 25.0%
ACAAAAAACACACAAGATAG+GGG + chr8.1:31043870-31043889 None:intergenic 30.0%
ACATGTGCACAAACTTTAAT+TGG - chr8.1:31044006-31044025 MS.gene012055:intron 30.0%
ATAACTACATCTTATGCAAG+AGG - chr8.1:31044030-31044049 MS.gene012055:intron 30.0%
ATAATAGTTTAAGGGGAATG+TGG + chr8.1:31043561-31043580 None:intergenic 30.0%
CACAAAAAACACACAAGATA+GGG + chr8.1:31043871-31043890 None:intergenic 30.0%
CTACATGTCTCACATTTAAA+TGG - chr8.1:31044140-31044159 MS.gene012055:intron 30.0%
GTGCTGTATTATTCATTTAG+GGG - chr8.1:31043953-31043972 MS.gene012055:intron 30.0%
TCACAAAAAACACACAAGAT+AGG + chr8.1:31043872-31043891 None:intergenic 30.0%
TTTAATCTTTCATCTCTAGC+AGG - chr8.1:31043772-31043791 MS.gene012055:intron 30.0%
! GATTTTCTTTGAATGGATCA+GGG - chr8.1:31044283-31044302 MS.gene012055:intron 30.0%
! TGATTTTCTTTGAATGGATC+AGG - chr8.1:31044282-31044301 MS.gene012055:intron 30.0%
CAAAAAACACACAAGATAGG+GGG + chr8.1:31043869-31043888 None:intergenic 35.0%
CATTAAAGGTTGTTTACCTC+AGG - chr8.1:31044072-31044091 MS.gene012055:intron 35.0%
GATGGAGCAAATTGAAACAA+AGG + chr8.1:31043716-31043735 None:intergenic 35.0%
TATCAAAGAGACTGGATCTA+CGG + chr8.1:31044479-31044498 None:intergenic 35.0%
TCACTTGGATTGAGCATTAA+AGG - chr8.1:31044058-31044077 MS.gene012055:intron 35.0%
TTGGTTGGATTGAAGAAAGT+GGG - chr8.1:31044339-31044358 MS.gene012055:intron 35.0%
TTTGGTTGGATTGAAGAAAG+TGG - chr8.1:31044338-31044357 MS.gene012055:intron 35.0%
! GAATTTGCTTCTGCTTTGTA+AGG - chr8.1:31043890-31043909 MS.gene012055:intron 35.0%
!! AGGTTTGTTACTTACAAGGA+TGG - chr8.1:31043917-31043936 MS.gene012055:intron 35.0%
!! TAGCAGGTTTGTTACTTACA+AGG - chr8.1:31043913-31043932 MS.gene012055:intron 35.0%
AGAAAGTGGGAAAAGGTGAT+TGG - chr8.1:31044352-31044371 MS.gene012055:intron 40.0%
ATGCAAGAGGAACTTTCACT+TGG - chr8.1:31044043-31044062 MS.gene012055:intron 40.0%
CATTCTCTCTGAGCAAACAA+TGG - chr8.1:31044117-31044136 MS.gene012055:intron 40.0%
GGATTGAAGAAAGTGGGAAA+AGG - chr8.1:31044345-31044364 MS.gene012055:intron 40.0%
!! GGAAGAGCACAAGATGTTTT+TGG - chr8.1:31044320-31044339 MS.gene012055:intron 40.0%
!!! GAGCACAAGATGTTTTTGGT+TGG - chr8.1:31044324-31044343 MS.gene012055:intron 40.0%
AATGTGGCTTACATGATCGG+GGG + chr8.1:31043545-31043564 None:intergenic 45.0%
CTTCTGCTTTGTAAGGTAGC+AGG - chr8.1:31043897-31043916 MS.gene012055:intron 45.0%
GAATGTGGCTTACATGATCG+GGG + chr8.1:31043546-31043565 None:intergenic 45.0%
GACGGCGATTGAGATTACTT+CGG + chr8.1:31044455-31044474 None:intergenic 45.0%
GGAATGTGGCTTACATGATC+GGG + chr8.1:31043547-31043566 None:intergenic 45.0%
GGGAATGTGGCTTACATGAT+CGG + chr8.1:31043548-31043567 None:intergenic 45.0%
GGTGGTGATATCAAAGAGAC+TGG + chr8.1:31044487-31044506 None:intergenic 45.0%
GTGATTGGAGAGGAATCTCA+AGG - chr8.1:31044367-31044386 MS.gene012055:intron 45.0%
TTACATGATCGGGGGATTGA+TGG + chr8.1:31043537-31043556 None:intergenic 45.0%
GGCGATTGAGATTACTTCGG+CGG + chr8.1:31044452-31044471 None:intergenic 50.0%
GGCTGAGAAGAGACTGCTAT+TGG - chr8.1:31043974-31043993 MS.gene012055:intron 50.0%
GTGGGAAAAGGTGATTGGAG+AGG - chr8.1:31044357-31044376 MS.gene012055:intron 50.0%
TGAATGGATCAGGGATTCCG+TGG - chr8.1:31044292-31044311 MS.gene012055:intron 50.0%
TTGTGCTCTTCCTCAGTCCA+CGG + chr8.1:31044312-31044331 None:intergenic 50.0%
! GCATGACTTGCAACATGCGT+AGG + chr8.1:31044414-31044433 None:intergenic 50.0%
AGAGACTGGATCTACGGCGA+CGG + chr8.1:31044473-31044492 None:intergenic 55.0%
! ATCAGGGATTCCGTGGACTG+AGG - chr8.1:31044299-31044318 MS.gene012055:intron 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31043519 31044512 31043519 ID=MS.gene012055
chr8.1 mRNA 31043519 31044512 31043519 ID=MS.gene012055.t1;Parent=MS.gene012055
chr8.1 exon 31044479 31044512 31044479 ID=MS.gene012055.t1.exon1;Parent=MS.gene012055.t1
chr8.1 CDS 31044479 31044512 31044479 ID=cds.MS.gene012055.t1;Parent=MS.gene012055.t1
chr8.1 exon 31043519 31043727 31043519 ID=MS.gene012055.t1.exon2;Parent=MS.gene012055.t1
chr8.1 CDS 31043519 31043727 31043519 ID=cds.MS.gene012055.t1;Parent=MS.gene012055.t1
Gene Sequence

>MS.gene012055

ATGGTGTTGCTCGGTCCATCAATCCCCCGATCATGTAAGCCACATTCCCCTTAAACTATTATATTCTTTTCAATTTTCAACATTTTATATCAGATATCACAACACTTATATTGCTCTATGAATAGAGTAATTATATTTAACATATGAAATTATAGACCATGAATATTTTTGCTTCAATATTAATTGTGTCTTGGCCTTTGTTTCAATTTGCTCCATCCAATTTTTATTATTTTTGCAAGCTTTTCTTTATAAGTTTAATCTTTCATCTCTAGCAGGCAATGTCTATCATGAAGATTCAAAACGAAAAAATGTTTGTTGCTGAGTTTTTGAGAGCTGTTTTTTGTGTGCCCCCTATCTTGTGTGTTTTTTGTGAATTTGCTTCTGCTTTGTAAGGTAGCAGGTTTGTTACTTACAAGGATGGCCAATTTTGAATTGTGCTGTATTATTCATTTAGGGGCTGAGAAGAGACTGCTATTGGAGTTAACATACATGTGCACAAACTTTAATTGGAATAACTACATCTTATGCAAGAGGAACTTTCACTTGGATTGAGCATTAAAGGTTGTTTACCTCAGGATTTTAAGTAGATATTTGACTGCATTCTCTCTGAGCAAACAATGGCTACATGTCTCACATTTAAATGGTTTTTGGCTATTGTTTAATAGAGATTCTATAAATTAACTAAAACTTGAAATTAACTTGATAATTTTTGTGTTTTTTCCTTTGTTTGAATTGATTGAAATTGAAAAGTAATTATTGTTATTGATTTTCTTTGAATGGATCAGGGATTCCGTGGACTGAGGAAGAGCACAAGATGTTTTTGGTTGGATTGAAGAAAGTGGGAAAAGGTGATTGGAGAGGAATCTCAAGGAACTCTGTGAAAACAAGAACGCCTACGCATGTTGCAAGTCATGCTCATAAGTACTTTCTCCGCCGAAGTAATCTCAATCGCCGTCGCCGTAGATCCAGTCTCTTTGATATCACCACCGACACG
Protein sequence

>MS.gene012055.t1

MVLLGPSIPRSWIPWTEEEHKMFLVGLKKVGKGDWRGISRNSVKTRTPTHVASHAHKYFLRRSNLNRRRRRSSLFDITTDT