Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012193.t1 | KEH32425.1 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 6.20E-30 | 140.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012193.t1 | A2XD79 | 47.0 | 83 | 41 | 2 | 32 | 111 | 96 | 178 | 3.6e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012193.t1 | A0A072V2U3 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 4.5e-30 | 140.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene012193.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012193.t1 | MTR_4g124950 | 70.175 | 114 | 22 | 2 | 1 | 106 | 1 | 110 | 8.13e-47 | 147 |
| MS.gene012193.t1 | MTR_4g124960 | 70.370 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 2.33e-45 | 145 |
| MS.gene012193.t1 | MTR_4g124960 | 70.755 | 106 | 30 | 1 | 1 | 106 | 67 | 171 | 1.70e-44 | 144 |
| MS.gene012193.t1 | MTR_2g042900 | 38.608 | 158 | 44 | 6 | 1 | 106 | 72 | 228 | 3.86e-22 | 87.8 |
| MS.gene012193.t1 | MTR_2g042900 | 43.617 | 94 | 33 | 3 | 1 | 74 | 72 | 165 | 3.15e-17 | 74.7 |
| MS.gene012193.t1 | MTR_5g013515 | 36.190 | 105 | 44 | 3 | 25 | 106 | 4 | 108 | 5.02e-14 | 64.3 |
| MS.gene012193.t1 | MTR_5g013530 | 40.625 | 96 | 40 | 3 | 30 | 108 | 17 | 112 | 1.22e-13 | 63.5 |
| MS.gene012193.t1 | MTR_5g013530 | 37.692 | 130 | 53 | 5 | 7 | 108 | 5 | 134 | 1.61e-13 | 63.5 |
| MS.gene012193.t1 | MTR_5g013520 | 37.234 | 94 | 42 | 2 | 30 | 106 | 10 | 103 | 7.89e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012193.t1 | AT1G19180 | 32.609 | 138 | 59 | 3 | 2 | 105 | 17 | 154 | 4.31e-11 | 57.4 |
| MS.gene012193.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
| MS.gene012193.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
Find 21 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCACTTCTTCGATTCA+TGG | 0.237608 | 8.1:+7104299 | MS.gene012193:CDS |
| AATGTCAACTGTGCAGTTTC+CGG | 0.323565 | 8.1:-7104005 | None:intergenic |
| TCTAGTAACACAAGTGGCTT+TGG | 0.360249 | 8.1:-7104905 | None:intergenic |
| CAACCACCTCCTACACTAAC+TGG | 0.374106 | 8.1:+7104143 | MS.gene012193:CDS |
| TTATTTCTTTAAAGAAGATA+TGG | 0.376656 | 8.1:+7104263 | MS.gene012193:intron |
| TAATGGAGAAGTTATTGTAT+TGG | 0.400656 | 8.1:+7104034 | MS.gene012193:CDS |
| TAACTCAATGGTTAACAAAC+CGG | 0.425259 | 8.1:+7103986 | MS.gene012193:CDS |
| GGTGGTTGAAGACTCATATC+AGG | 0.439597 | 8.1:-7104128 | None:intergenic |
| AGCTCCTCAACTTTGTCAGC+AGG | 0.455494 | 8.1:-7104065 | None:intergenic |
| TTACAGAACTAATAACTCAA+TGG | 0.476118 | 8.1:+7103974 | MS.gene012193:intron |
| TTCTTCGATTCATGGAGAAA+AGG | 0.478429 | 8.1:+7104307 | MS.gene012193:CDS |
| CAATTACCAGTTAGTGTAGG+AGG | 0.522487 | 8.1:-7104149 | None:intergenic |
| CTGTGGATTCTTTGTCTCCA+AGG | 0.531713 | 8.1:+7103830 | MS.gene012193:CDS |
| TCATGGAGAAAAGGAAAGCC+AGG | 0.536399 | 8.1:+7104316 | MS.gene012193:CDS |
| CATTTGCAAGCACTCAAACT+CGG | 0.560353 | 8.1:+7104093 | MS.gene012193:CDS |
| CTCATATCAGGAATAACTGA+AGG | 0.598557 | 8.1:-7104116 | None:intergenic |
| TCAATTTCTAGTAACACAAG+TGG | 0.602083 | 8.1:-7104911 | None:intergenic |
| GTTTGTGGGAAATAAGTCCA+TGG | 0.603000 | 8.1:-7103785 | None:intergenic |
| TTACCAGTTAGTGTAGGAGG+TGG | 0.608915 | 8.1:-7104146 | None:intergenic |
| GAGCAATTACCAGTTAGTGT+AGG | 0.610011 | 8.1:-7104152 | None:intergenic |
| CTTGGAGACAAAGAATCCAC+AGG | 0.648213 | 8.1:-7103829 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATTTTGAAACAAATAA+AGG | + | chr8.1:7104187-7104206 | MS.gene012193:intron | 10.0% |
| !! | AAGAAATAAAATTTATGATC+AGG | - | chr8.1:7104252-7104271 | None:intergenic | 15.0% |
| !!! | AAGGAAATATTTTTTGTATT+AGG | + | chr8.1:7104206-7104225 | MS.gene012193:intron | 15.0% |
| !!! | AGGAAATATTTTTTGTATTA+GGG | + | chr8.1:7104207-7104226 | MS.gene012193:intron | 15.0% |
| !!! | ATTATGAATATTTTGATTAC+CGG | - | chr8.1:7104612-7104631 | None:intergenic | 15.0% |
| !!! | CAATCAACTAATATTTTTTT+GGG | + | chr8.1:7104495-7104514 | MS.gene012193:intron | 15.0% |
| !!! | TAAATTCAAGTTTTTATTCA+AGG | + | chr8.1:7104357-7104376 | MS.gene012193:intron | 15.0% |
| !!! | TCAATCAACTAATATTTTTT+TGG | + | chr8.1:7104494-7104513 | MS.gene012193:intron | 15.0% |
| !!! | TTATTTCTTTAAAGAAGATA+TGG | + | chr8.1:7104263-7104282 | MS.gene012193:intron | 15.0% |
| ! | TAATGGAGAAGTTATTGTAT+TGG | + | chr8.1:7104034-7104053 | MS.gene012193:CDS | 25.0% |
| ! | TTACAGAACTAATAACTCAA+TGG | + | chr8.1:7103974-7103993 | MS.gene012193:intron | 25.0% |
| ! | TTGTTTCAACATTTCAATCA+AGG | - | chr8.1:7104472-7104491 | None:intergenic | 25.0% |
| !!! | CAGTTGACATTGTTTTATAA+TGG | + | chr8.1:7104017-7104036 | MS.gene012193:CDS | 25.0% |
| !!! | GTAAAATGTGATTTTTGAGA+TGG | - | chr8.1:7104845-7104864 | None:intergenic | 25.0% |
| !!! | TTGAATTTAATTTGTCTGAG+AGG | - | chr8.1:7104346-7104365 | None:intergenic | 25.0% |
| GTTGTCGTAACTTTGTAATT+TGG | + | chr8.1:7104739-7104758 | MS.gene012193:intron | 30.0% | |
| TAACTCAATGGTTAACAAAC+CGG | + | chr8.1:7103986-7104005 | MS.gene012193:CDS | 30.0% | |
| !! | AGTAGTTGATGATATGCATA+AGG | - | chr8.1:7104812-7104831 | None:intergenic | 30.0% |
| !! | TCAATTTCTAGTAACACAAG+TGG | - | chr8.1:7104914-7104933 | None:intergenic | 30.0% |
| ATAGACAGAGAAATGGAGAA+GGG | - | chr8.1:7104449-7104468 | None:intergenic | 35.0% | |
| ATCAAGGATAGACAGAGAAA+TGG | - | chr8.1:7104456-7104475 | None:intergenic | 35.0% | |
| CTCATATCAGGAATAACTGA+AGG | - | chr8.1:7104119-7104138 | None:intergenic | 35.0% | |
| TAGAATTGGATGGAATCAGT+TGG | - | chr8.1:7104871-7104890 | None:intergenic | 35.0% | |
| TTCTTCGATTCATGGAGAAA+AGG | + | chr8.1:7104307-7104326 | MS.gene012193:CDS | 35.0% | |
| ! | AACTGATTCCATCCAATTCT+AGG | + | chr8.1:7104870-7104889 | MS.gene012193:intron | 35.0% |
| ! | AGAAGCACAAAAACATACCT+TGG | - | chr8.1:7103850-7103869 | None:intergenic | 35.0% |
| !!! | ACTTTTTTGCACACAAGCAA+TGG | - | chr8.1:7103913-7103932 | None:intergenic | 35.0% |
| !!! | CAACTTTCGTGTTGTTTTCA+CGG | - | chr8.1:7104723-7104742 | None:intergenic | 35.0% |
| !!! | CTTTTTTGCACACAAGCAAT+GGG | - | chr8.1:7103912-7103931 | None:intergenic | 35.0% |
| !!! | TTTTTTGCACACAAGCAATG+GGG | - | chr8.1:7103911-7103930 | None:intergenic | 35.0% |
| !!! | TTTTTTGGTGTCACGTTTGT+GGG | - | chr8.1:7103802-7103821 | None:intergenic | 35.0% |
| AATGTCAACTGTGCAGTTTC+CGG | - | chr8.1:7104008-7104027 | None:intergenic | 40.0% | |
| ACCAAAAAACGTTACACCTG+TGG | + | chr8.1:7103813-7103832 | MS.gene012193:CDS | 40.0% | |
| AGCTTCACTTCTTCGATTCA+TGG | + | chr8.1:7104299-7104318 | MS.gene012193:CDS | 40.0% | |
| CAAGCAAAATCAAGGACTTC+CGG | + | chr8.1:7104590-7104609 | MS.gene012193:intron | 40.0% | |
| CAATTACCAGTTAGTGTAGG+AGG | - | chr8.1:7104152-7104171 | None:intergenic | 40.0% | |
| CACTATTTATGTTCCTCTCG+TGG | - | chr8.1:7104658-7104677 | None:intergenic | 40.0% | |
| CATTTGCAAGCACTCAAACT+CGG | + | chr8.1:7104093-7104112 | MS.gene012193:CDS | 40.0% | |
| GAGCAATTACCAGTTAGTGT+AGG | - | chr8.1:7104155-7104174 | None:intergenic | 40.0% | |
| GATAGACAGAGAAATGGAGA+AGG | - | chr8.1:7104450-7104469 | None:intergenic | 40.0% | |
| GCAATGGGGCAAAACAAAAA+TGG | - | chr8.1:7103897-7103916 | None:intergenic | 40.0% | |
| TCTAGTAACACAAGTGGCTT+TGG | - | chr8.1:7104908-7104927 | None:intergenic | 40.0% | |
| TTGTGCTCATGCTCTTACAA+GGG | - | chr8.1:7104684-7104703 | None:intergenic | 40.0% | |
| ! | TTTTCCTGCTGACAAAGTTG+AGG | + | chr8.1:7104061-7104080 | MS.gene012193:CDS | 40.0% |
| !!! | GTTTTTTGGTGTCACGTTTG+TGG | - | chr8.1:7103803-7103822 | None:intergenic | 40.0% |
| !!! | TCCACAGGTGTAACGTTTTT+TGG | - | chr8.1:7103817-7103836 | None:intergenic | 40.0% |
| !!! | TTCGTGTTGTTTTCACGGTA+CGG | - | chr8.1:7104718-7104737 | None:intergenic | 40.0% |
| CTCTTCAGCAAGCCTAGAAT+TGG | - | chr8.1:7104885-7104904 | None:intergenic | 45.0% | |
| CTGTGGATTCTTTGTCTCCA+AGG | + | chr8.1:7103830-7103849 | MS.gene012193:CDS | 45.0% | |
| CTTCGACGCAAGCAAAATCA+AGG | + | chr8.1:7104582-7104601 | MS.gene012193:intron | 45.0% | |
| CTTGGAGACAAAGAATCCAC+AGG | - | chr8.1:7103832-7103851 | None:intergenic | 45.0% | |
| GGTGGTTGAAGACTCATATC+AGG | - | chr8.1:7104131-7104150 | None:intergenic | 45.0% | |
| GTTGTGCTCATGCTCTTACA+AGG | - | chr8.1:7104685-7104704 | None:intergenic | 45.0% | |
| TCAGCAAGCCTAGAATTGGA+TGG | - | chr8.1:7104881-7104900 | None:intergenic | 45.0% | |
| TCATAGTCAAGAACCACGAG+AGG | + | chr8.1:7104642-7104661 | MS.gene012193:intron | 45.0% | |
| TCATGGAGAAAAGGAAAGCC+AGG | + | chr8.1:7104316-7104335 | MS.gene012193:CDS | 45.0% | |
| TTACCAGTTAGTGTAGGAGG+TGG | - | chr8.1:7104149-7104168 | None:intergenic | 45.0% | |
| ! | ATTTGTCTGAGAGGCATACC+TGG | - | chr8.1:7104337-7104356 | None:intergenic | 45.0% |
| ! | CTTGATTTTGCTTGCGTCGA+AGG | - | chr8.1:7104584-7104603 | None:intergenic | 45.0% |
| AGCTCCTCAACTTTGTCAGC+AGG | - | chr8.1:7104068-7104087 | None:intergenic | 50.0% | |
| CAACCACCTCCTACACTAAC+TGG | + | chr8.1:7104143-7104162 | MS.gene012193:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 7103787 | 7104940 | 7103787 | ID=MS.gene012193 |
| chr8.1 | mRNA | 7103787 | 7104940 | 7103787 | ID=MS.gene012193.t1;Parent=MS.gene012193 |
| chr8.1 | exon | 7103787 | 7103851 | 7103787 | ID=MS.gene012193.t1.exon1;Parent=MS.gene012193.t1 |
| chr8.1 | CDS | 7103787 | 7103851 | 7103787 | ID=cds.MS.gene012193.t1;Parent=MS.gene012193.t1 |
| chr8.1 | exon | 7103980 | 7104164 | 7103980 | ID=MS.gene012193.t1.exon2;Parent=MS.gene012193.t1 |
| chr8.1 | CDS | 7103980 | 7104164 | 7103980 | ID=cds.MS.gene012193.t1;Parent=MS.gene012193.t1 |
| chr8.1 | exon | 7104277 | 7104337 | 7104277 | ID=MS.gene012193.t1.exon3;Parent=MS.gene012193.t1 |
| chr8.1 | CDS | 7104277 | 7104337 | 7104277 | ID=cds.MS.gene012193.t1;Parent=MS.gene012193.t1 |
| chr8.1 | exon | 7104892 | 7104940 | 7104892 | ID=MS.gene012193.t1.exon4;Parent=MS.gene012193.t1 |
| chr8.1 | CDS | 7104892 | 7104940 | 7104892 | ID=cds.MS.gene012193.t1;Parent=MS.gene012193.t1 |
| Gene Sequence |
| Protein sequence |
