AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012219.t1 MTR_4g125490 98.910 367 3 1 1 367 1 366 0.0 750
MS.gene012219.t1 MTR_2g041430 61.680 381 103 12 5 369 3 356 7.08e-140 402
MS.gene012219.t1 MTR_2g041430 61.804 377 101 12 5 365 3 352 2.99e-138 397
MS.gene012219.t1 MTR_2g041430 61.804 377 101 12 5 365 3 352 3.16e-138 397
MS.gene012219.t1 MTR_8g020560 42.740 365 155 16 1 338 1 338 1.17e-68 219
MS.gene012219.t1 MTR_0001s0490 39.266 354 147 14 9 352 25 320 2.62e-55 185
MS.gene012219.t1 MTR_5g027030 60.550 109 31 2 9 117 16 112 1.31e-39 143
MS.gene012219.t1 MTR_1g017490 56.897 116 44 2 7 117 9 123 1.43e-39 147
MS.gene012219.t1 MTR_1g017490 56.897 116 44 2 7 117 142 256 1.88e-39 148
MS.gene012219.t1 MTR_7g104050 53.383 133 41 4 6 124 68 193 5.86e-37 137
MS.gene012219.t1 MTR_7g104050 53.383 133 41 4 6 124 68 193 7.19e-37 137
MS.gene012219.t1 MTR_3g092330 48.739 119 42 4 9 127 113 212 4.13e-27 112
MS.gene012219.t1 MTR_8g035910 45.536 112 47 3 6 117 46 143 4.98e-21 94.4
MS.gene012219.t1 MTR_8g035910 43.750 80 34 2 73 152 297 365 4.99e-13 70.5
MS.gene012219.t1 MTR_8g035640 64.103 39 14 0 73 111 51 89 1.66e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012219.t1 AT3G13960 71.053 114 27 3 7 117 14 124 4.08e-52 178
MS.gene012219.t1 AT2G06200 66.667 120 29 2 7 115 43 162 3.48e-48 164
MS.gene012219.t1 AT2G06200 66.667 120 29 2 7 115 4 123 4.55e-48 163
MS.gene012219.t1 AT4G37740 55.118 127 52 3 9 134 164 286 3.54e-41 152
MS.gene012219.t1 AT2G36400 57.377 122 43 3 10 125 77 195 6.64e-37 138
MS.gene012219.t1 AT2G22840 57.143 112 44 2 7 117 131 239 1.29e-34 134
MS.gene012219.t1 AT3G52910 53.390 118 39 3 10 117 83 194 3.85e-33 127
MS.gene012219.t1 AT5G53660 50.000 110 38 2 8 117 58 150 6.34e-27 110
MS.gene012219.t1 AT2G06200 92.157 51 2 1 67 115 1 51 5.60e-26 102
MS.gene012219.t1 AT4G24150 42.149 121 64 2 2 117 104 223 7.75e-23 99.4
MS.gene012219.t1 AT4G24150 42.149 121 64 2 2 117 142 261 1.33e-22 99.4
MS.gene012219.t1 AT4G24150 36.986 146 61 3 2 117 142 286 8.73e-21 94.0
MS.gene012219.t1 AT2G45480 41.441 111 64 1 5 114 19 129 1.73e-18 85.5
MS.gene012219.t1 AT2G45480 41.441 111 64 1 5 114 17 127 6.59e-18 85.1
MS.gene012219.t1 AT2G45480 41.935 93 42 3 70 155 301 388 8.78e-13 69.7
MS.gene012219.t1 AT2G45480 41.441 111 64 1 5 114 19 129 6.91e-18 85.1
MS.gene012219.t1 AT2G45480 41.935 93 42 3 70 155 303 390 9.08e-13 69.7

Find 80 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGAGTTTCTGCTGCTTT+TGG 0.148849 8.3:+4568093 None:intergenic
TGAAGGAACAGGTATGTTTA+TGG 0.231611 8.3:-4568430 MS.gene012219:intron
TGCCACTGAGTTGGAGTAAA+AGG 0.276188 8.3:+4569506 None:intergenic
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8.3:+4569218 None:intergenic
CCCTTGTTTCTTGACAATAC+TGG 0.290985 8.3:-4568849 MS.gene012219:CDS
CAGAAACTCATGGCTTGATT+TGG 0.299180 8.3:-4568083 MS.gene012219:CDS
GCTTTCTTCACTGAACCTTC+TGG 0.302685 8.3:-4568382 MS.gene012219:CDS
CTTTCACAGTACTTTGAATC+TGG 0.319625 8.3:+4569155 None:intergenic
AAAAGAGGAAGAGGAGCTTA+TGG 0.356828 8.3:+4568300 None:intergenic
CTTCTTCAATGGATGATTCA+TGG 0.368355 8.3:-4568339 MS.gene012219:CDS
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8.3:+4569219 None:intergenic
ATCTACAAATACATGGCTTC+TGG 0.368517 8.3:-4569461 MS.gene012219:CDS
TTGTCTTGAAATGAGAAGTT+AGG 0.391585 8.3:+4568879 None:intergenic
AGAGGAAAGGGTGGTTAAGA+TGG 0.391821 8.3:+4568925 None:intergenic
GAACTATTTACCAATGGGAT+TGG 0.400450 8.3:-4569255 MS.gene012219:CDS
TTTAAACAGAGAAATTGTTC+TGG 0.401538 8.3:-4568280 MS.gene012219:CDS
TTCATCGTTTCTTTGATGAA+TGG 0.411092 8.3:-4568120 MS.gene012219:CDS
AACTATTTACCAATGGGATT+GGG 0.420553 8.3:-4569254 MS.gene012219:CDS
CCATTTACCTCGTTGGTTTG+TGG 0.423824 8.3:+4567988 None:intergenic
GCCTTGAGGATGGAGGGTTA+TGG 0.442680 8.3:+4568901 None:intergenic
AAGTTTATCAGCTTCTTCAA+TGG 0.445043 8.3:-4568350 MS.gene012219:CDS
GAGGGTTATGGTAGAGGAAA+GGG 0.446918 8.3:+4568913 None:intergenic
TTGATAGTGAGTAGAAGATC+AGG 0.446923 8.3:+4569425 None:intergenic
AAGCATAAGAAGAGTGTTGA+GGG 0.452542 8.3:+4568952 None:intergenic
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8.3:+4569212 None:intergenic
ACAATTTCTCTGTTTAAAAG+AGG 0.457057 8.3:+4568285 None:intergenic
GAGGAAAGGGTGGTTAAGAT+GGG 0.457253 8.3:+4568926 None:intergenic
GAGTAGAAGATCAGGAGGAA+TGG 0.465599 8.3:+4569433 None:intergenic
GTAAGAGTACTCATTGTCAT+TGG 0.465700 8.3:+4568252 None:intergenic
CTTCCACAGGCTTTCTTGAA+CGG 0.472599 8.3:+4569111 None:intergenic
GGATGGAACTATTTACCAAT+GGG 0.479254 8.3:-4569260 MS.gene012219:CDS
CAATGATGAATGGAAGAAAC+AGG 0.479723 8.3:-4569532 MS.gene012219:CDS
GAAGAACAGATGGAAAGAAA+TGG 0.480263 8.3:-4569199 MS.gene012219:CDS
ATAACAACAACACTGATTGC+AGG 0.480287 8.3:-4568812 MS.gene012219:intron
AAGAAGAGTGTTGAGGGTAA+TGG 0.482978 8.3:+4568958 None:intergenic
GATCAAGGTTGTTACATCTC+AGG 0.490399 8.3:-4568193 MS.gene012219:CDS
TCTCTGTTTAAAAGAGGAAG+AGG 0.498513 8.3:+4568291 None:intergenic
TAACAAACAACAATGATGAA+TGG 0.501615 8.3:-4569542 None:intergenic
AGGATGGAGGGTTATGGTAG+AGG 0.503413 8.3:+4568907 None:intergenic
GTGTGGTGCATGCAGTTGGA+TGG 0.510680 8.3:-4569277 MS.gene012219:intron
TCAAGCTCTTGCCACTGAGT+TGG 0.513392 8.3:+4569497 None:intergenic
TTATGGACTGAAAGAGGAGG+TGG 0.522907 8.3:-4568413 MS.gene012219:CDS
GAAGAGGAGCTTATGGTAAG+TGG 0.526833 8.3:+4568307 None:intergenic
AAGATTGTTGAAGTAGCAGC+AGG 0.534940 8.3:+4569059 None:intergenic
ATCATGAGAAGATGTAGGAA+TGG 0.536076 8.3:+4568027 None:intergenic
ACCAGTATTGTCAAGAAACA+AGG 0.541001 8.3:+4568848 None:intergenic
AAACAACCACAGCAAGATCA+AGG 0.541674 8.3:-4568208 MS.gene012219:CDS
AGAGAAACCATTTACCTCGT+TGG 0.542197 8.3:+4567981 None:intergenic
GTGAAATCATGAGAAGATGT+AGG 0.543173 8.3:+4568022 None:intergenic
GTGAAAGGCACATGCATAGA+GGG 0.547205 8.3:-4569139 MS.gene012219:CDS
GAAGCATAAGAAGAGTGTTG+AGG 0.548399 8.3:+4568951 None:intergenic
GGAGGGTTATGGTAGAGGAA+AGG 0.556801 8.3:+4568912 None:intergenic
GAACAGATGGAAAGAAATGG+AGG 0.559737 8.3:-4569196 MS.gene012219:CDS
TGGATGGAACTATTTACCAA+TGG 0.564553 8.3:-4569261 MS.gene012219:CDS
GAAGAAACATTCATGAAACA+TGG 0.569282 8.3:-4568166 MS.gene012219:CDS
ATAGTGAGTAGAAGATCAGG+AGG 0.571348 8.3:+4569428 None:intergenic
CAGATTCAAAGTACTGTGAA+AGG 0.571794 8.3:-4569154 MS.gene012219:CDS
GGGAGGTGCAGAAGAACAGA+TGG 0.577454 8.3:-4569209 MS.gene012219:CDS
AAACTTCTTATAGTTCCAGA+AGG 0.579842 8.3:+4568367 None:intergenic
AAGTTAGGCCTTGAGGATGG+AGG 0.587194 8.3:+4568894 None:intergenic
ACCATAACCCTCCATCCTCA+AGG 0.592083 8.3:-4568902 MS.gene012219:CDS
AAATGAGAAGTTAGGCCTTG+AGG 0.594153 8.3:+4568887 None:intergenic
AGTTAGGCCTTGAGGATGGA+GGG 0.597805 8.3:+4568895 None:intergenic
GTATGTTTATGGACTGAAAG+AGG 0.601366 8.3:-4568419 MS.gene012219:CDS
AAGAAACATTCATGAAACAT+GGG 0.603745 8.3:-4568165 MS.gene012219:CDS
GAGAAGTTAGGCCTTGAGGA+TGG 0.604526 8.3:+4568891 None:intergenic
CCAAAAGCAGCAGAAACTCA+TGG 0.605608 8.3:-4568093 MS.gene012219:CDS
AGCTCTTATCTACAAATACA+TGG 0.606523 8.3:-4569468 MS.gene012219:CDS
AGTGTTGTTGTTATTGTGAG+AGG 0.610925 8.3:+4568821 None:intergenic
GGAGATTGAAAGCTGAGTGG+TGG 0.613550 8.3:+4568048 None:intergenic
TAACAACCTTGATCTTGCTG+TGG 0.626299 8.3:+4568202 None:intergenic
TGTGAAAGGCACATGCATAG+AGG 0.626931 8.3:-4569140 MS.gene012219:CDS
AAACCGTTCAAGAAAGCCTG+TGG 0.641388 8.3:-4569114 MS.gene012219:CDS
TGTTTATGGACTGAAAGAGG+AGG 0.650765 8.3:-4568416 MS.gene012219:CDS
CCAGTATTGTCAAGAAACAA+GGG 0.652005 8.3:+4568849 None:intergenic
AAATAGACCCAGAGCCAGGG+AGG 0.659120 8.3:-4569226 MS.gene012219:CDS
GGTTATGGTAGAGGAAAGGG+TGG 0.659870 8.3:+4568916 None:intergenic
TTTAGTTCCACAAACCAACG+AGG 0.729736 8.3:-4567995 MS.gene012219:intron
AATGGAGATTGAAAGCTGAG+TGG 0.781428 8.3:+4568045 None:intergenic
TTACAGTGTTGAGACTGATG+AGG 0.793602 8.3:+4569371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATGGTAAAAATTATTAT+TGG + chr8.3:4569342-4569361 None:intergenic 15.0%
!! TAATTGAATGTAAATTATCA+TGG - chr8.3:4569477-4569496 MS.gene012219:CDS 15.0%
!! TATATATAGTCATTCATATA+TGG + chr8.3:4567785-4567804 None:intergenic 15.0%
!!! AGTTTTTCTCAAATTATTAT+CGG + chr8.3:4569279-4569298 None:intergenic 15.0%
!! AGAGAACTTATAAAAATAAG+TGG - chr8.3:4568494-4568513 MS.gene012219:intron 20.0%
!!! CTTATCGATTATAAACTTAT+AGG + chr8.3:4568436-4568455 None:intergenic 20.0%
!!! TTAAGTTGTTTATTCAAACA+TGG + chr8.3:4568364-4568383 None:intergenic 20.0%
!!! TTTATGAAAGATTCTCTTTT+TGG - chr8.3:4568647-4568666 MS.gene012219:intron 20.0%
! AAGAAACATTCATGAAACAT+GGG - chr8.3:4568950-4568969 MS.gene012219:CDS 25.0%
! ACAATTTCTCTGTTTAAAAG+AGG + chr8.3:4568833-4568852 None:intergenic 25.0%
! CTTTAAACCAACACATATTT+AGG - chr8.3:4568338-4568357 MS.gene012219:CDS 25.0%
! TGAGTTTCGCAAATTATTAT+TGG + chr8.3:4569457-4569476 None:intergenic 25.0%
! TTTAAACAGAGAAATTGTTC+TGG - chr8.3:4568835-4568854 MS.gene012219:CDS 25.0%
!!! TATTATTGGTGTCAATGTAT+CGG + chr8.3:4569443-4569462 None:intergenic 25.0%
AAACGATGAAAAATCTTCTG+AGG + chr8.3:4568985-4569004 None:intergenic 30.0%
AAACTTCTTATAGTTCCAGA+AGG + chr8.3:4568751-4568770 None:intergenic 30.0%
AACTATTTACCAATGGGATT+GGG - chr8.3:4567861-4567880 MS.gene012219:intron 30.0%
AAGTTTATCAGCTTCTTCAA+TGG - chr8.3:4568765-4568784 MS.gene012219:intron 30.0%
AGCTCTTATCTACAAATACA+TGG - chr8.3:4567647-4567666 MS.gene012219:intron 30.0%
AGTGATTAGTGATTTCTCAT+TGG - chr8.3:4569503-4569522 MS.gene012219:CDS 30.0%
GAAGAAACATTCATGAAACA+TGG - chr8.3:4568949-4568968 MS.gene012219:CDS 30.0%
TCAAATAGTCCAATCCAAAA+AGG - chr8.3:4568586-4568605 MS.gene012219:intron 30.0%
TTCATCGTTTCTTTGATGAA+TGG - chr8.3:4568995-4569014 MS.gene012219:CDS 30.0%
TTGTCTTGAAATGAGAAGTT+AGG + chr8.3:4568239-4568258 None:intergenic 30.0%
TTTCCTCAAATTATCACCTA+TGG + chr8.3:4569216-4569235 None:intergenic 30.0%
! GGTATTGTTGTTATCTTTGA+AGG - chr8.3:4568668-4568687 MS.gene012219:intron 30.0%
! TATGTATGCACTTGTTGATT+TGG - chr8.3:4567816-4567835 MS.gene012219:intron 30.0%
!! ATTTCTCATTGGCTTGAATT+TGG - chr8.3:4569514-4569533 MS.gene012219:CDS 30.0%
AACATGGCCTAAATATGTGT+TGG + chr8.3:4568348-4568367 None:intergenic 35.0%
ACCAGTATTGTCAAGAAACA+AGG + chr8.3:4568270-4568289 None:intergenic 35.0%
ATCATGAGAAGATGTAGGAA+TGG + chr8.3:4569091-4569110 None:intergenic 35.0%
ATCTACAAATACATGGCTTC+TGG - chr8.3:4567654-4567673 MS.gene012219:intron 35.0%
CAATCCAAAAAGGTTCATGA+TGG - chr8.3:4568596-4568615 MS.gene012219:intron 35.0%
CCAGTATTGTCAAGAAACAA+GGG + chr8.3:4568269-4568288 None:intergenic 35.0%
CTTCTTCAATGGATGATTCA+TGG - chr8.3:4568776-4568795 MS.gene012219:intron 35.0%
CTTTCACAGTACTTTGAATC+TGG + chr8.3:4567963-4567982 None:intergenic 35.0%
GAACTATTTACCAATGGGAT+TGG - chr8.3:4567860-4567879 MS.gene012219:intron 35.0%
GAAGAACAGATGGAAAGAAA+TGG - chr8.3:4567916-4567935 MS.gene012219:intron 35.0%
GGATGGAACTATTTACCAAT+GGG - chr8.3:4567855-4567874 MS.gene012219:intron 35.0%
GTGAAATCATGAGAAGATGT+AGG + chr8.3:4569096-4569115 None:intergenic 35.0%
TCTCTGTTTAAAAGAGGAAG+AGG + chr8.3:4568827-4568846 None:intergenic 35.0%
TGAAGGAACAGGTATGTTTA+TGG - chr8.3:4568685-4568704 MS.gene012219:intron 35.0%
TGGATGGAACTATTTACCAA+TGG - chr8.3:4567854-4567873 MS.gene012219:intron 35.0%
! AGTGTTGTTGTTATTGTGAG+AGG + chr8.3:4568297-4568316 None:intergenic 35.0%
! ATAACAACAACACTGATTGC+AGG - chr8.3:4568303-4568322 MS.gene012219:CDS 35.0%
! ATTTTTCTTCCCAATCCCAT+TGG + chr8.3:4567873-4567892 None:intergenic 35.0%
! CATCATGAACCTTTTTGGAT+TGG + chr8.3:4568598-4568617 None:intergenic 35.0%
! GTATGTTTATGGACTGAAAG+AGG - chr8.3:4568696-4568715 MS.gene012219:intron 35.0%
! GTTGTTATCTTTGAAGGAAC+AGG - chr8.3:4568674-4568693 MS.gene012219:intron 35.0%
!! AAGCATAAGAAGAGTGTTGA+GGG + chr8.3:4568166-4568185 None:intergenic 35.0%
!! ATTATCGGTGTTGATGTGTT+TGG + chr8.3:4569264-4569283 None:intergenic 35.0%
!! CAGATTCAAAGTACTGTGAA+AGG - chr8.3:4567961-4567980 MS.gene012219:intron 35.0%
!! GTAAGAGTACTCATTGTCAT+TGG + chr8.3:4568866-4568885 None:intergenic 35.0%
!! GTTGTTTTCAAAACTTCCAC+AGG + chr8.3:4568020-4568039 None:intergenic 35.0%
!! TTGATAGTGAGTAGAAGATC+AGG + chr8.3:4567693-4567712 None:intergenic 35.0%
!!! CTTGAATTTGGTTTTGCAGA+TGG - chr8.3:4569526-4569545 MS.gene012219:CDS 35.0%
AAAAGAGGAAGAGGAGCTTA+TGG + chr8.3:4568818-4568837 None:intergenic 40.0%
AAACAACCACAGCAAGATCA+AGG - chr8.3:4568907-4568926 MS.gene012219:CDS 40.0%
AAATGAGAAGTTAGGCCTTG+AGG + chr8.3:4568231-4568250 None:intergenic 40.0%
AAGATTGTTGAAGTAGCAGC+AGG + chr8.3:4568059-4568078 None:intergenic 40.0%
AAGCTATCGTTGAGACTGAA+TGG - chr8.3:4568466-4568485 MS.gene012219:intron 40.0%
AATGGAGATTGAAAGCTGAG+TGG + chr8.3:4569073-4569092 None:intergenic 40.0%
AGAGAAACCATTTACCTCGT+TGG + chr8.3:4569137-4569156 None:intergenic 40.0%
ATAGTGAGTAGAAGATCAGG+AGG + chr8.3:4567690-4567709 None:intergenic 40.0%
CAGAAACTCATGGCTTGATT+TGG - chr8.3:4569032-4569051 MS.gene012219:CDS 40.0%
GAACAGATGGAAAGAAATGG+AGG - chr8.3:4567919-4567938 MS.gene012219:intron 40.0%
GATCAAGGTTGTTACATCTC+AGG - chr8.3:4568922-4568941 MS.gene012219:CDS 40.0%
TAACAACCTTGATCTTGCTG+TGG + chr8.3:4568916-4568935 None:intergenic 40.0%
TCGCCATAGGTGATAATTTG+AGG - chr8.3:4569210-4569229 MS.gene012219:CDS 40.0%
TGACTAGAGCATGTTGACAA+TGG + chr8.3:4569358-4569377 None:intergenic 40.0%
! AGCACCATCATGAACCTTTT+TGG + chr8.3:4568603-4568622 None:intergenic 40.0%
! ATCCTTTTACTCCAACTCAG+TGG - chr8.3:4567607-4567626 MS.gene012219:intron 40.0%
! CCCTTGTTTCTTGACAATAC+TGG - chr8.3:4568266-4568285 MS.gene012219:CDS 40.0%
! TGTTTATGGACTGAAAGAGG+AGG - chr8.3:4568699-4568718 MS.gene012219:intron 40.0%
! TTACAGTGTTGAGACTGATG+AGG + chr8.3:4567747-4567766 None:intergenic 40.0%
! TTTAGTTCCACAAACCAACG+AGG - chr8.3:4569120-4569139 MS.gene012219:CDS 40.0%
!! AAGAAGAGTGTTGAGGGTAA+TGG + chr8.3:4568160-4568179 None:intergenic 40.0%
!! ATGCACTTGTTGATTTGGTG+TGG - chr8.3:4567821-4567840 MS.gene012219:intron 40.0%
!! GAAGCATAAGAAGAGTGTTG+AGG + chr8.3:4568167-4568186 None:intergenic 40.0%
!!! CATGAGTTTCTGCTGCTTTT+GGG + chr8.3:4569024-4569043 None:intergenic 40.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr8.3:4568001-4568020 MS.gene012219:CDS 45.0%
AGAAAAATAGACCCAGAGCC+AGG - chr8.3:4567885-4567904 MS.gene012219:intron 45.0%
AGAGGAAAGGGTGGTTAAGA+TGG + chr8.3:4568193-4568212 None:intergenic 45.0%
CCAAAAGCAGCAGAAACTCA+TGG - chr8.3:4569022-4569041 MS.gene012219:CDS 45.0%
CCACAAACCAACGAGGTAAA+TGG - chr8.3:4569127-4569146 MS.gene012219:CDS 45.0%
CTTCCACAGGCTTTCTTGAA+CGG + chr8.3:4568007-4568026 None:intergenic 45.0%
GAAAAATAGACCCAGAGCCA+GGG - chr8.3:4567886-4567905 MS.gene012219:intron 45.0%
GAAGAGGAGCTTATGGTAAG+TGG + chr8.3:4568811-4568830 None:intergenic 45.0%
GAGGAAAGGGTGGTTAAGAT+GGG + chr8.3:4568192-4568211 None:intergenic 45.0%
GAGGGTTATGGTAGAGGAAA+GGG + chr8.3:4568205-4568224 None:intergenic 45.0%
GAGTAGAAGATCAGGAGGAA+TGG + chr8.3:4567685-4567704 None:intergenic 45.0%
GCTTTCTTCACTGAACCTTC+TGG - chr8.3:4568733-4568752 MS.gene012219:intron 45.0%
TGCTTCAAAGCTATGAGCCA+CGG + chr8.3:4569397-4569416 None:intergenic 45.0%
! GTGAAAGGCACATGCATAGA+GGG - chr8.3:4567976-4567995 MS.gene012219:intron 45.0%
! TGCCACTGAGTTGGAGTAAA+AGG + chr8.3:4567612-4567631 None:intergenic 45.0%
! TGTGAAAGGCACATGCATAG+AGG - chr8.3:4567975-4567994 MS.gene012219:intron 45.0%
! TTATGGACTGAAAGAGGAGG+TGG - chr8.3:4568702-4568721 MS.gene012219:intron 45.0%
!! CCATTTACCTCGTTGGTTTG+TGG + chr8.3:4569130-4569149 None:intergenic 45.0%
!!! CCATGAGTTTCTGCTGCTTT+TGG + chr8.3:4569025-4569044 None:intergenic 45.0%
!!! ATAAACATTTTAATAAAAAA+AGG + chr8.3:4568632-4568651 None:intergenic 5.0%
AAGTTAGGCCTTGAGGATGG+AGG + chr8.3:4568224-4568243 None:intergenic 50.0%
ACCATAACCCTCCATCCTCA+AGG - chr8.3:4568213-4568232 MS.gene012219:CDS 50.0%
AGGATGGAGGGTTATGGTAG+AGG + chr8.3:4568211-4568230 None:intergenic 50.0%
AGTTAGGCCTTGAGGATGGA+GGG + chr8.3:4568223-4568242 None:intergenic 50.0%
GAGAAGTTAGGCCTTGAGGA+TGG + chr8.3:4568227-4568246 None:intergenic 50.0%
GGAGATTGAAAGCTGAGTGG+TGG + chr8.3:4569070-4569089 None:intergenic 50.0%
GGAGGGTTATGGTAGAGGAA+AGG + chr8.3:4568206-4568225 None:intergenic 50.0%
GGTTATGGTAGAGGAAAGGG+TGG + chr8.3:4568202-4568221 None:intergenic 50.0%
TCAAGCTCTTGCCACTGAGT+TGG + chr8.3:4567621-4567640 None:intergenic 50.0%
! TTTGGTGTGGTGCATGCAGT+TGG - chr8.3:4567834-4567853 MS.gene012219:intron 50.0%
!!! GAGTTTCTGCTGCTTTTGGG+TGG + chr8.3:4569021-4569040 None:intergenic 50.0%
AAATAGACCCAGAGCCAGGG+AGG - chr8.3:4567889-4567908 MS.gene012219:intron 55.0%
CGACACTGACACATCGCCAT+AGG - chr8.3:4569197-4569216 MS.gene012219:CDS 55.0%
GCCTTGAGGATGGAGGGTTA+TGG + chr8.3:4568217-4568236 None:intergenic 55.0%
GCTCTAGTCAGTGTTGTGCC+TGG - chr8.3:4569368-4569387 MS.gene012219:intron 55.0%
GTGTGGTGCATGCAGTTGGA+TGG - chr8.3:4567838-4567857 MS.gene012219:intron 55.0%
TCTGTTCTTCTGCACCTCCC+TGG + chr8.3:4567906-4567925 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG - chr8.3:4567906-4567925 MS.gene012219:intron 55.0%
AGCTATGAGCCACGGACACC+AGG + chr8.3:4569389-4569408 None:intergenic 60.0%
AGTGTTGTGCCTGGTGTCCG+TGG - chr8.3:4569377-4569396 MS.gene012219:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG + chr8.3:4567899-4567918 None:intergenic 60.0%
CTTCTGCACCTCCCTGGCTC+TGG + chr8.3:4567900-4567919 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 4567585 4569552 4567585 ID=MS.gene012219
chr8.3 mRNA 4567585 4569552 4567585 ID=MS.gene012219.t1;Parent=MS.gene012219
chr8.3 exon 4569375 4569552 4569375 ID=MS.gene012219.t1.exon1;Parent=MS.gene012219.t1
chr8.3 CDS 4569375 4569552 4569375 ID=cds.MS.gene012219.t1;Parent=MS.gene012219.t1
chr8.3 exon 4568813 4569284 4568813 ID=MS.gene012219.t1.exon2;Parent=MS.gene012219.t1
chr8.3 CDS 4568813 4569284 4568813 ID=cds.MS.gene012219.t1;Parent=MS.gene012219.t1
chr8.3 exon 4567996 4568447 4567996 ID=MS.gene012219.t1.exon3;Parent=MS.gene012219.t1
chr8.3 CDS 4567996 4568447 4567996 ID=cds.MS.gene012219.t1;Parent=MS.gene012219.t1
chr8.3 exon 4567585 4567592 4567585 ID=MS.gene012219.t1.exon4;Parent=MS.gene012219.t1
chr8.3 CDS 4567585 4567592 4567585 ID=cds.MS.gene012219.t1;Parent=MS.gene012219.t1
Gene Sequence

>MS.gene012219

ATGATGAATGGAAGAAACAGGTATCCTTTTACTCCAACTCAGTGGCAAGAGCTTGAACATCAAGCTCTTATCTACAAATACATGGCTTCTGGTATTTCCATTCCTCCTGATCTTCTACTCACTATCAAAAGAAGCTACTTAGACTCTTCAAGACTCTCACCTCATCAGTCTCAACACTGTAAGTACTATTCTATGATCCATATATGAATGACTATATATATCAGCTGAATATATGTATGCACTTGTTGATTTGGTGTGGTGCATGCAGTTGGATGGAACTATTTACCAATGGGATTGGGAAGAAAAATAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGAAAGAAATGGAGGTGTTCAAAAGAAGCATATCCAGATTCAAAGTACTGTGAAAGGCACATGCATAGAGGGAAAAACCGTTCAAGAAAGCCTGTGGAAGTTTTGAAAACAACAACAAACACAGATGCTCCTGCTGCTACTTCAACAATCTTATCAATCACTAAAAATAGTAGTCTTGATTCTGATCATAACACTGCAGCACTAACATCAACAACTCATGACACTAATTACCATTACCCTCAACACTCTTCTTATGCTTCTTCCCATCTTAACCACCCTTTCCTCTACCATAACCCTCCATCCTCAAGGCCTAACTTCTCATTTCAAGACAATAGTGCTCCCTTGTTTCTTGACAATACTGGTTCTTCCTCTCACAATAACAACAACACTGATTGCAGGTTACTAACTTTTCTTTAAACCAACACATATTTAGGCCATGTTTGAATAAACAACTTAAAATAATCTTGCTTACATCATAAACACTTATCATATATGTAAGCTGTTTTCCTATAAGTTTATAATCGATAAGTTGTTTTCATAAGCTATCGTTGAGACTGAATGGAGACTAGAGAACTTATAAAAATAAGTGGAAAACAGTTTATGAACATGTCATAAGTTGTTTCTATAAGTTTTCTCAAACATTCTGTAAGTAGAATAGTTCAAATAGTCCAATCCAAAAAGGTTCATGATGGTGCTTGCTTTCCTTTTTTTATTAAAATGTTTATGAAAGATTCTCTTTTTGGTATTGTTGTTATCTTTGAAGGAACAGGTATGTTTATGGACTGAAAGAGGAGGTGGATGAACATGCTTTCTTCACTGAACCTTCTGGAACTATAAGAAGTTTATCAGCTTCTTCAATGGATGATTCATGGCAACTCACACCACTTACCATAAGCTCCTCTTCCTCTTTTAAACAGAGAAATTGTTCTGGTTTATCCAATGACAATGAGTACTCTTACTTGCAACTTGACAACAACTCAAAACAACCACAGCAAGATCAAGGTTGTTACATCTCAGGTAGTGAAGAAACATTCATGAAACATGGGAAAGAAGAACCTCAGAAGATTTTTCATCGTTTCTTTGATGAATGGCCACCCAAAAGCAGCAGAAACTCATGGCTTGATTTGGATGATAAATCATCCACCACTCAGCTTTCAATCTCCATTCCTACATCTTCTCATGATTTCACAACTTTTAGTTCCACAAACCAACGAGGTAAATGGTTTCTCTTCACTCTATTCTCTTTCAAGCATTGTTACAAACATGTACACGACACTGACACATCGCCATAGGTGATAATTTGAGGAAAACTCTATTGTCTTTGAAGCATTGTTCCAAACACATCAACACCGATAATAATTTGAGAAAAACTTTATTGTCTTTGAAGCTCTGTAGCACTAACATTTCTAGCACCAATAATAATTTTTACCATTGTCAACATGCTCTAGTCAGTGTTGTGCCTGGTGTCCGTGGCTCATAGCTTTGAAGCAATGTTACAAACACATACACAACACCGATACATTGACACCAATAATAATTTGCGAAACTCATAATTGAATGTAAATTATCATGGATTAGTGATTAGTGATTTCTCATTGGCTTGAATTTGGTTTTGCAGATGGTTGA
Protein sequence

>MS.gene012219.t1

MMNGRNRYPFTPTQWQELEHQALIYKYMASGISIPPDLLLTIKRSYLDSSRLSPHQSQHFGWNYLPMGLGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVLKTTTNTDAPAATSTILSITKNSSLDSDHNTAALTSTTHDTNYHYPQHSSYASSHLNHPFLYHNPPSSRPNFSFQDNSAPLFLDNTGSSSHNNNNTDCRNRYVYGLKEEVDEHAFFTEPSGTIRSLSASSMDDSWQLTPLTISSSSSFKQRNCSGLSNDNEYSYLQLDNNSKQPQQDQGCYISGSEETFMKHGKEEPQKIFHRFFDEWPPKSSRNSWLDLDDKSSTTQLSISIPTSSHDFTTFSSTNQRDG