AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012268

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012268.t1 MTR_3g104550 41.504 359 166 9 87 419 33 373 8.77e-78 246
MS.gene012268.t1 MTR_7g095680 47.273 275 124 4 68 321 7 281 5.05e-75 238
MS.gene012268.t1 MTR_1g102860 47.619 252 115 6 64 305 6 250 6.43e-70 224
MS.gene012268.t1 MTR_5g029680 51.220 205 84 3 87 277 21 223 2.37e-64 214
MS.gene012268.t1 MTR_1g071360 47.727 220 106 3 87 304 19 231 4.23e-63 207
MS.gene012268.t1 MTR_1g071360 47.727 220 106 3 87 304 16 228 7.73e-63 207
MS.gene012268.t1 MTR_4g077970 51.852 216 82 5 99 298 11 220 1.27e-61 207
MS.gene012268.t1 MTR_1g061170 49.500 200 96 1 87 281 36 235 2.35e-61 206
MS.gene012268.t1 MTR_8g087540 43.939 198 101 2 99 286 11 208 5.84e-51 177
MS.gene012268.t1 MTR_2g100680 44.041 193 105 1 98 287 29 221 2.40e-50 176
MS.gene012268.t1 MTR_6g043060 41.004 239 118 5 100 335 22 240 2.10e-48 168
MS.gene012268.t1 MTR_2g100670 41.038 212 113 4 94 301 27 230 1.27e-46 162
MS.gene012268.t1 MTR_2g100670 41.038 212 113 4 94 301 135 338 1.25e-45 163
MS.gene012268.t1 MTR_8g083100 45.604 182 95 2 101 279 23 203 2.55e-45 164
MS.gene012268.t1 MTR_8g105780 47.120 191 87 3 100 276 22 212 9.24e-45 159
MS.gene012268.t1 MTR_6g086805 45.263 190 99 2 100 285 11 199 4.31e-44 157
MS.gene012268.t1 MTR_2g017890 43.243 185 95 3 99 273 14 198 6.41e-41 149
MS.gene012268.t1 MTR_3g101870 60.000 110 43 1 94 203 18 126 1.51e-38 143
MS.gene012268.t1 MTR_5g010680 63.441 93 34 0 98 190 22 114 1.31e-37 140
MS.gene012268.t1 MTR_5g017470 62.766 94 35 0 97 190 6 99 1.86e-37 138
MS.gene012268.t1 MTR_5g089170 64.516 93 33 0 98 190 23 115 4.97e-37 135
MS.gene012268.t1 MTR_7g091370 53.571 112 48 1 91 198 9 120 4.20e-36 132
MS.gene012268.t1 MTR_1g100777 58.065 93 39 0 98 190 16 108 5.95e-34 129
MS.gene012268.t1 MTR_0020s0020 59.140 93 38 0 98 190 31 123 8.34e-33 126
MS.gene012268.t1 MTR_4g088900 56.701 97 38 1 98 190 31 127 2.19e-31 119
MS.gene012268.t1 MTR_1g061170 41.732 127 69 1 160 281 14 140 1.27e-23 101
MS.gene012268.t1 MTR_4g045757 56.716 67 29 0 91 157 5 71 1.95e-19 82.8
MS.gene012268.t1 MTR_5g047190 45.263 95 45 2 108 195 1 95 7.94e-19 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012268.t1 AT2G26150 54.054 222 90 4 87 299 31 249 6.66e-76 240
MS.gene012268.t1 AT2G26150 54.054 222 90 4 87 299 31 249 6.66e-76 240
MS.gene012268.t1 AT5G54070 52.713 258 108 7 68 322 46 292 1.31e-73 234
MS.gene012268.t1 AT3G22830 46.586 249 117 4 73 306 34 281 1.71e-73 236
MS.gene012268.t1 AT3G51910 48.792 207 96 3 90 295 19 216 1.19e-62 203
MS.gene012268.t1 AT5G16820 47.391 230 94 3 70 282 2 221 1.75e-62 209
MS.gene012268.t1 AT5G16820 47.391 230 94 3 70 282 2 221 1.75e-62 209
MS.gene012268.t1 AT4G17750 50.000 216 82 3 98 292 50 260 6.20e-62 208
MS.gene012268.t1 AT1G32330 46.753 231 107 3 87 301 24 254 2.71e-61 206
MS.gene012268.t1 AT1G32330 46.753 231 107 3 87 301 24 254 4.15e-61 206
MS.gene012268.t1 AT3G63350 39.850 266 150 3 73 329 1 265 1.10e-59 196
MS.gene012268.t1 AT3G02990 51.579 190 81 2 99 277 22 211 2.89e-57 195
MS.gene012268.t1 AT5G43840 45.503 189 96 1 86 274 5 186 2.01e-54 182
MS.gene012268.t1 AT5G03720 47.291 203 101 3 86 282 41 243 4.69e-54 185
MS.gene012268.t1 AT5G03720 47.291 203 101 3 86 282 41 243 4.69e-54 185
MS.gene012268.t1 AT1G67970 48.108 185 88 2 100 276 19 203 2.30e-49 172
MS.gene012268.t1 AT4G13980 43.169 183 101 1 101 280 24 206 7.51e-46 165
MS.gene012268.t1 AT3G24520 41.000 200 102 2 100 299 17 200 1.09e-42 153
MS.gene012268.t1 AT4G18880 44.865 185 94 2 92 268 7 191 1.04e-37 141
MS.gene012268.t1 AT1G46264 61.386 101 39 0 90 190 24 124 1.49e-37 139
MS.gene012268.t1 AT5G45710 41.808 177 96 1 99 268 12 188 3.75e-37 138
MS.gene012268.t1 AT5G45710 41.808 177 96 1 99 268 12 188 3.75e-37 138
MS.gene012268.t1 AT2G41690 61.290 93 36 0 98 190 38 130 1.13e-35 132
MS.gene012268.t1 AT4G11660 61.290 93 36 0 98 190 57 149 3.86e-35 134
MS.gene012268.t1 AT4G36990 62.366 93 35 0 98 190 12 104 5.88e-35 131
MS.gene012268.t1 AT5G62020 58.416 101 41 1 98 198 21 120 6.61e-35 131
MS.gene012268.t1 AT2G26150 37.705 183 82 5 126 299 35 194 1.02e-26 108
MS.gene012268.t1 AT2G26150 37.705 183 82 5 126 299 35 194 1.02e-26 108
MS.gene012268.t1 AT4G18870 42.045 88 51 0 100 187 146 233 2.42e-18 85.5
MS.gene012268.t1 AT4G18870 43.678 87 48 1 101 187 14 99 2.51e-17 82.4
MS.gene012268.t1 AT1G77570 37.778 90 53 2 101 187 27 116 3.77e-12 64.3
MS.gene012268.t1 AT4G19630 38.542 96 52 3 97 187 8 101 2.41e-11 61.6

Find 86 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCAAAAGGCCATTGTTTA+TGG 0.168710 8.1:+6049748 MS.gene012268:CDS
GTACTTAGGAAGAAGAATTT+TGG 0.203486 8.1:-6048568 None:intergenic
GAAACAATCGGGTCAGTTTC+CGG 0.255207 8.1:-6048495 None:intergenic
AGATCAGAACATGTTGAAAT+TGG 0.272987 8.1:+6049607 MS.gene012268:CDS
CTCCATAAACAATGGCCTTT+TGG 0.280439 8.1:-6049750 None:intergenic
TGTAACCCTTCCATTGGTTT+AGG 0.283169 8.1:-6048420 None:intergenic
TAATATCTATCTTGAATTAG+AGG 0.304710 8.1:+6050135 MS.gene012268:CDS
TGTTATGTCAGAGAAGCTTT+TGG 0.350236 8.1:+6050069 MS.gene012268:CDS
TCTGTCACATGCAGGGGTTT+AGG 0.362833 8.1:+6049420 MS.gene012268:intron
GATAAGTAAGCCAACAGATT+GGG 0.381656 8.1:+6050162 MS.gene012268:CDS
CCTTGTGTTCCAAGCAATCT+AGG 0.385680 8.1:-6049836 None:intergenic
TTCTGTTGTTGATGAAGAAA+TGG 0.389880 8.1:+6050099 MS.gene012268:CDS
CGTTGGGAGTTTGCAAATGA+AGG 0.392464 8.1:+6049458 MS.gene012268:CDS
TATTCAACTCACAAATGTTC+AGG 0.406470 8.1:+6049667 MS.gene012268:CDS
TAATGTTGATTATAGGAGTC+AGG 0.409936 8.1:+6049862 MS.gene012268:CDS
AAACTGACCCGATTGTTTCA+TGG 0.421449 8.1:+6048499 MS.gene012268:CDS
TGGATCTGCTAGTGGATTGG+TGG 0.433958 8.1:+6050186 MS.gene012268:CDS
GGTGGTTACGACGGTGAAAT+CGG 0.435742 8.1:+6048177 MS.gene012268:CDS
GTTTGAAATGGTGGAGGATC+CGG 0.440036 8.1:+6048476 MS.gene012268:CDS
GTTAAAAGACCTAGATTGCT+TGG 0.444562 8.1:+6049827 MS.gene012268:CDS
AAGGTACTAATGTTGATTAT+AGG 0.444857 8.1:+6049855 MS.gene012268:CDS
AAATATGAACACTGGTAGAA+TGG 0.455673 8.1:+6049937 MS.gene012268:CDS
GAGCCACTCCATGAAACAAT+CGG 0.466527 8.1:-6048507 None:intergenic
TCTCAAGAAGACGTTTGAAA+TGG 0.467110 8.1:+6048464 MS.gene012268:CDS
TGATAAGTAAGCCAACAGAT+TGG 0.468059 8.1:+6050161 MS.gene012268:CDS
TCCTCACCAAAATGGCCAAA+AGG 0.470362 8.1:+6049735 MS.gene012268:CDS
ACCACCTGTTTCCACTCCTA+TGG 0.477088 8.1:+6049961 MS.gene012268:CDS
TAGCTCTTTACATGACTTGA+GGG 0.478947 8.1:+6049997 MS.gene012268:CDS
GAAGGTTGATTCAGACCGTT+GGG 0.482934 8.1:+6049442 MS.gene012268:CDS
CAAATGAAGGATTTCAAGCA+GGG 0.484602 8.1:+6049471 MS.gene012268:CDS
GACTTACATAGGTGTTGAGC+TGG 0.485810 8.1:-6048623 None:intergenic
ATTTGTTGAAGAACATAAGA+AGG 0.488953 8.1:+6049501 MS.gene012268:CDS
ACCTCATGTAACCCTTCCAT+TGG 0.493568 8.1:-6048426 None:intergenic
TCTTCCATAGGAGTGGAAAC+AGG 0.496566 8.1:-6049965 None:intergenic
GCACTGTCGTTGAATAACTC+TGG 0.499469 8.1:-6048234 None:intergenic
GGGTGGATCTGCTAGTGGAT+TGG 0.502731 8.1:+6050183 MS.gene012268:CDS
GCAAATGAAGGATTTCAAGC+AGG 0.502811 8.1:+6049470 MS.gene012268:CDS
ATTCAAAAGATTAAGCGAAA+AGG 0.508398 8.1:+6049779 MS.gene012268:CDS
TTACATGACTTGAGGGCTTG+TGG 0.510182 8.1:+6050004 MS.gene012268:CDS
AACAAAATGGCGGTGGCACA+CGG 0.510707 8.1:+6048156 None:intergenic
ACTTTGCAATCTGAATTGAA+TGG 0.510922 8.1:+6049902 MS.gene012268:CDS
TTATTATGCTTAAAGTACTT+AGG 0.510929 8.1:-6048582 None:intergenic
CAAGAAGACGTTTGAAATGG+TGG 0.512314 8.1:+6048467 MS.gene012268:CDS
CGCAATTCATATCAAAGAAG+AGG 0.515252 8.1:+6048353 MS.gene012268:CDS
GGAAGGTTGATTCAGACCGT+TGG 0.515312 8.1:+6049441 MS.gene012268:CDS
TCAAACGTCTTCTTGAGAAA+CGG 0.525722 8.1:-6048459 None:intergenic
AATTGGAAATTCTGAAGCTA+AGG 0.526195 8.1:+6049624 MS.gene012268:CDS
TTCGGTTGAAACGTCGTCGT+AGG 0.530109 8.1:-6048274 None:intergenic
GTTTCCACTCCTATGGAAGA+TGG 0.537583 8.1:+6049968 MS.gene012268:CDS
GTAGCTCTTTACATGACTTG+AGG 0.539985 8.1:+6049996 MS.gene012268:CDS
GGATCTGCTAGTGGATTGGT+GGG 0.540365 8.1:+6050187 MS.gene012268:CDS
ACTCAGGTTTCCTAAACCAA+TGG 0.540511 8.1:+6048410 MS.gene012268:CDS
AGGTTTCCTAAACCAATGGA+AGG 0.554746 8.1:+6048414 MS.gene012268:CDS
AGCCACTCCATGAAACAATC+GGG 0.558154 8.1:-6048506 None:intergenic
TCCACTTCAGATTCAACGCA+AGG 0.559576 8.1:-6049572 None:intergenic
CTTCATTTGCAAACTCCCAA+CGG 0.561522 8.1:-6049457 None:intergenic
GACCCGATTGTTTCATGGAG+TGG 0.564406 8.1:+6048504 MS.gene012268:CDS
CCTAGATTGCTTGGAACACA+AGG 0.567220 8.1:+6049836 MS.gene012268:CDS
ACAAATAAACAAAATGGCGG+TGG 0.567551 8.1:+6048149 None:intergenic
TCACATGCAGGGGTTTAGGA+AGG 0.572288 8.1:+6049424 MS.gene012268:intron
CTACGCAGTCCATCTTCCAT+AGG 0.572989 8.1:-6049977 None:intergenic
ATAAGTAAGCCAACAGATTG+GGG 0.574399 8.1:+6050163 MS.gene012268:CDS
AAGTATAACAAACTGCATCA+AGG 0.578236 8.1:+6049530 MS.gene012268:CDS
AATGTTGATTATAGGAGTCA+GGG 0.582952 8.1:+6049863 MS.gene012268:CDS
TTTCCTGAAAATATGAACAC+TGG 0.585214 8.1:+6049929 MS.gene012268:CDS
CTAACGCTTCATCTTCACTC+AGG 0.586633 8.1:+6048394 MS.gene012268:CDS
GCAGATCCACCCCAATCTGT+TGG 0.588399 8.1:-6050172 None:intergenic
GTCTTCTTGAGAAACGGTGG+TGG 0.589533 8.1:-6048453 None:intergenic
CAGTCCATCTTCCATAGGAG+TGG 0.594383 8.1:-6049972 None:intergenic
ATGGAAGGGTTACATGAGGT+TGG 0.601818 8.1:+6048429 MS.gene012268:CDS
TCCTTGCGTTGAATCTGAAG+TGG 0.604555 8.1:+6049571 MS.gene012268:CDS
GAAGAGGTTCTTGAAGAAGA+CGG 0.609483 8.1:+6048369 MS.gene012268:CDS
ATTATCTGCTCCATAAACAA+TGG 0.622243 8.1:-6049758 None:intergenic
GATTGGGGTGGATCTGCTAG+TGG 0.632406 8.1:+6050178 MS.gene012268:CDS
GGTTTCCTAAACCAATGGAA+GGG 0.633308 8.1:+6048415 MS.gene012268:CDS
GAAGACGTTTGAAATGGTGG+AGG 0.633963 8.1:+6048470 MS.gene012268:CDS
CATGACTTGAGGGCTTGTGG+TGG 0.643012 8.1:+6050007 MS.gene012268:CDS
GTGGCACACGGTGGTTACGA+CGG 0.645161 8.1:+6048168 MS.gene012268:CDS
AACGTCTTCTTGAGAAACGG+TGG 0.656391 8.1:-6048456 None:intergenic
AGTAAGCCAACAGATTGGGG+TGG 0.660771 8.1:+6050166 MS.gene012268:CDS
ACCAATGGAAGGGTTACATG+AGG 0.667282 8.1:+6048425 MS.gene012268:CDS
TCCATAGGAGTGGAAACAGG+TGG 0.685790 8.1:-6049962 None:intergenic
GGTTACGACGGTGAAATCGG+TGG 0.688203 8.1:+6048180 MS.gene012268:CDS
AGTTGATGCTAATATAGACA+TGG 0.689403 8.1:+6049805 MS.gene012268:CDS
TATAACAAACTGCATCAAGG+AGG 0.715319 8.1:+6049533 MS.gene012268:CDS
AAAATGGCGGTGGCACACGG+TGG 0.747694 8.1:+6048159 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATGATTCATATTTAT+AGG + chr8.1:6048809-6048828 MS.gene012268:intron 10.0%
!! TAAAATGATTCATATTTATA+GGG + chr8.1:6048810-6048829 MS.gene012268:intron 10.0%
!!! TTTTTTCATGTTTTTTTACA+TGG - chr8.1:6049111-6049130 None:intergenic 15.0%
!! AAATCCAGAATAAATTCAAA+TGG - chr8.1:6049164-6049183 None:intergenic 20.0%
!! TAATATCTATCTTGAATTAG+AGG + chr8.1:6050135-6050154 MS.gene012268:CDS 20.0%
!!! AGAATTTTTTTGACTTACAT+AGG - chr8.1:6048637-6048656 None:intergenic 20.0%
!!! GCAAAAAACAAAATAGATTT+TGG - chr8.1:6049243-6049262 None:intergenic 20.0%
!!! TGTACAATTTAAATCAGTTT+TGG + chr8.1:6048699-6048718 MS.gene012268:intron 20.0%
!!! TTATTATGCTTAAAGTACTT+AGG - chr8.1:6048585-6048604 None:intergenic 20.0%
! ACATTAAATTCGAACTTCAT+TGG + chr8.1:6049056-6049075 MS.gene012268:intron 25.0%
! ATTCAAAAGATTAAGCGAAA+AGG + chr8.1:6049779-6049798 MS.gene012268:CDS 25.0%
!! AAGGTACTAATGTTGATTAT+AGG + chr8.1:6049855-6049874 MS.gene012268:CDS 25.0%
!! ATTTGTTGAAGAACATAAGA+AGG + chr8.1:6049501-6049520 MS.gene012268:CDS 25.0%
!!! AATAACTCTGGTTTTATTGA+TGG - chr8.1:6048225-6048244 None:intergenic 25.0%
!!! ATAACTCTGGTTTTATTGAT+GGG - chr8.1:6048224-6048243 None:intergenic 25.0%
!!! TAGTACTGATTCTTTTCTTT+CGG - chr8.1:6048295-6048314 None:intergenic 25.0%
!!! TTACATGAACTCACTTTTAT+AGG + chr8.1:6049015-6049034 MS.gene012268:intron 25.0%
!!! TTTTTTGCAACCACAAAAAT+AGG + chr8.1:6049255-6049274 MS.gene012268:intron 25.0%
AAATATGAACACTGGTAGAA+TGG + chr8.1:6049937-6049956 MS.gene012268:CDS 30.0%
AAATCAACTCAATGCATGTT+TGG - chr8.1:6048754-6048773 None:intergenic 30.0%
AAGTATAACAAACTGCATCA+AGG + chr8.1:6049530-6049549 MS.gene012268:CDS 30.0%
ACTTTGCAATCTGAATTGAA+TGG + chr8.1:6049902-6049921 MS.gene012268:CDS 30.0%
AGATCAGAACATGTTGAAAT+TGG + chr8.1:6049607-6049626 MS.gene012268:CDS 30.0%
ATTATCTGCTCCATAAACAA+TGG - chr8.1:6049761-6049780 None:intergenic 30.0%
TATTCAACTCACAAATGTTC+AGG + chr8.1:6049667-6049686 MS.gene012268:CDS 30.0%
TTCGCCATTTGAATTTATTC+TGG + chr8.1:6049157-6049176 MS.gene012268:intron 30.0%
TTTCCTGAAAATATGAACAC+TGG + chr8.1:6049929-6049948 MS.gene012268:CDS 30.0%
! AATGTTGATTATAGGAGTCA+GGG + chr8.1:6049863-6049882 MS.gene012268:CDS 30.0%
! AGTTGATGCTAATATAGACA+TGG + chr8.1:6049805-6049824 MS.gene012268:CDS 30.0%
! GTACTTAGGAAGAAGAATTT+TGG - chr8.1:6048571-6048590 None:intergenic 30.0%
! TAATGTTGATTATAGGAGTC+AGG + chr8.1:6049862-6049881 MS.gene012268:CDS 30.0%
! TTCTGTTGTTGATGAAGAAA+TGG + chr8.1:6050099-6050118 MS.gene012268:CDS 30.0%
!! AATTGGAAATTCTGAAGCTA+AGG + chr8.1:6049624-6049643 MS.gene012268:CDS 30.0%
!! TTAAATCAGTTTTGGCATCA+CGG + chr8.1:6048707-6048726 MS.gene012268:intron 30.0%
!!! TCGTGATAGTTTTGTTGTTT+GGG + chr8.1:6048530-6048549 MS.gene012268:CDS 30.0%
AATGCATGTTTGGTATCATG+TGG - chr8.1:6048744-6048763 None:intergenic 35.0%
ACCTTCTTCTTCTTCTTCTT+CGG - chr8.1:6048319-6048338 None:intergenic 35.0%
ATAAGTAAGCCAACAGATTG+GGG + chr8.1:6050163-6050182 MS.gene012268:CDS 35.0%
CAAATGAAGGATTTCAAGCA+GGG + chr8.1:6049471-6049490 MS.gene012268:CDS 35.0%
CGCAATTCATATCAAAGAAG+AGG + chr8.1:6048353-6048372 MS.gene012268:CDS 35.0%
GATAAGTAAGCCAACAGATT+GGG + chr8.1:6050162-6050181 MS.gene012268:CDS 35.0%
GTTAAAAGACCTAGATTGCT+TGG + chr8.1:6049827-6049846 MS.gene012268:CDS 35.0%
TAGCTCTTTACATGACTTGA+GGG + chr8.1:6049997-6050016 MS.gene012268:CDS 35.0%
TATAACAAACTGCATCAAGG+AGG + chr8.1:6049533-6049552 MS.gene012268:CDS 35.0%
TATAGGGAGCTATGAATGTT+TGG + chr8.1:6048826-6048845 MS.gene012268:intron 35.0%
TCAAACGTCTTCTTGAGAAA+CGG - chr8.1:6048462-6048481 None:intergenic 35.0%
TCTCAAGAAGACGTTTGAAA+TGG + chr8.1:6048464-6048483 MS.gene012268:CDS 35.0%
TGATAAGTAAGCCAACAGAT+TGG + chr8.1:6050161-6050180 MS.gene012268:CDS 35.0%
TTATGAATCTGTCACATGCA+GGG + chr8.1:6049413-6049432 MS.gene012268:intron 35.0%
TTTATGAATCTGTCACATGC+AGG + chr8.1:6049412-6049431 MS.gene012268:intron 35.0%
TTTGCAACCACAAAAATAGG+TGG + chr8.1:6049258-6049277 MS.gene012268:intron 35.0%
! CTACCAGTGTTCATATTTTC+AGG - chr8.1:6049935-6049954 None:intergenic 35.0%
! GTTATGTCAGAGAAGCTTTT+GGG + chr8.1:6050070-6050089 MS.gene012268:CDS 35.0%
! TGTTATGTCAGAGAAGCTTT+TGG + chr8.1:6050069-6050088 MS.gene012268:CDS 35.0%
! TTATGTCAGAGAAGCTTTTG+GGG + chr8.1:6050071-6050090 MS.gene012268:CDS 35.0%
! TTCAACATCTCCTCTAAAAG+AGG - chr8.1:6048854-6048873 None:intergenic 35.0%
!! ATGTTTGGTTCCTCTTTTAG+AGG + chr8.1:6048841-6048860 MS.gene012268:intron 35.0%
!!! CTCGTGATAGTTTTGTTGTT+TGG + chr8.1:6048529-6048548 MS.gene012268:CDS 35.0%
!!! CTGGTTTTATTGATGGGATT+AGG - chr8.1:6048218-6048237 None:intergenic 35.0%
!!! GCTATTTTTCCTCACCAAAA+TGG + chr8.1:6049727-6049746 MS.gene012268:CDS 35.0%
!!! TTAGTCTCCACCTATTTTTG+TGG - chr8.1:6049268-6049287 None:intergenic 35.0%
AAACTGACCCGATTGTTTCA+TGG + chr8.1:6048499-6048518 MS.gene012268:CDS 40.0%
ACCGAAGAAGAAGAAGAAGA+AGG + chr8.1:6048315-6048334 MS.gene012268:CDS 40.0%
ACTCAGGTTTCCTAAACCAA+TGG + chr8.1:6048410-6048429 MS.gene012268:CDS 40.0%
AGGTTTCCTAAACCAATGGA+AGG + chr8.1:6048414-6048433 MS.gene012268:CDS 40.0%
CTTCATTTGCAAACTCCCAA+CGG - chr8.1:6049460-6049479 None:intergenic 40.0%
GAAGAGGTTCTTGAAGAAGA+CGG + chr8.1:6048369-6048388 MS.gene012268:CDS 40.0%
GCAAATGAAGGATTTCAAGC+AGG + chr8.1:6049470-6049489 MS.gene012268:CDS 40.0%
GGTGGAGACTAAAAATCAAG+AGG + chr8.1:6049276-6049295 MS.gene012268:intron 40.0%
GGTTTCCTAAACCAATGGAA+GGG + chr8.1:6048415-6048434 MS.gene012268:CDS 40.0%
GTAGCTCTTTACATGACTTG+AGG + chr8.1:6049996-6050015 MS.gene012268:CDS 40.0%
TATGAATCTGTCACATGCAG+GGG + chr8.1:6049414-6049433 MS.gene012268:intron 40.0%
! CAAGAAGACGTTTGAAATGG+TGG + chr8.1:6048467-6048486 MS.gene012268:CDS 40.0%
! CTCCATAAACAATGGCCTTT+TGG - chr8.1:6049753-6049772 None:intergenic 40.0%
! TATGTCAGAGAAGCTTTTGG+GGG + chr8.1:6050072-6050091 MS.gene012268:CDS 40.0%
!! TGTAACCCTTCCATTGGTTT+AGG - chr8.1:6048423-6048442 None:intergenic 40.0%
AACGTCTTCTTGAGAAACGG+TGG - chr8.1:6048459-6048478 None:intergenic 45.0%
ACCAATGGAAGGGTTACATG+AGG + chr8.1:6048425-6048444 MS.gene012268:CDS 45.0%
ACCTCATGTAACCCTTCCAT+TGG - chr8.1:6048429-6048448 None:intergenic 45.0%
AGCCACTCCATGAAACAATC+GGG - chr8.1:6048509-6048528 None:intergenic 45.0%
ATGGAAGGGTTACATGAGGT+TGG + chr8.1:6048429-6048448 MS.gene012268:CDS 45.0%
CCTAGATTGCTTGGAACACA+AGG + chr8.1:6049836-6049855 MS.gene012268:CDS 45.0%
CCTTGTGTTCCAAGCAATCT+AGG - chr8.1:6049839-6049858 None:intergenic 45.0%
CGTTGGGAGTTTGCAAATGA+AGG + chr8.1:6049458-6049477 MS.gene012268:CDS 45.0%
CTAACGCTTCATCTTCACTC+AGG + chr8.1:6048394-6048413 MS.gene012268:CDS 45.0%
GAAACAATCGGGTCAGTTTC+CGG - chr8.1:6048498-6048517 None:intergenic 45.0%
GAGCCACTCCATGAAACAAT+CGG - chr8.1:6048510-6048529 None:intergenic 45.0%
GCACTGTCGTTGAATAACTC+TGG - chr8.1:6048237-6048256 None:intergenic 45.0%
GGCCAAAAGGCCATTGTTTA+TGG + chr8.1:6049748-6049767 MS.gene012268:CDS 45.0%
GTTTCCACTCCTATGGAAGA+TGG + chr8.1:6049968-6049987 MS.gene012268:CDS 45.0%
TCCACTTCAGATTCAACGCA+AGG - chr8.1:6049575-6049594 None:intergenic 45.0%
TCCTCACCAAAATGGCCAAA+AGG + chr8.1:6049735-6049754 MS.gene012268:CDS 45.0%
TCCTTGCGTTGAATCTGAAG+TGG + chr8.1:6049571-6049590 MS.gene012268:CDS 45.0%
TCTTCCATAGGAGTGGAAAC+AGG - chr8.1:6049968-6049987 None:intergenic 45.0%
TTACATGACTTGAGGGCTTG+TGG + chr8.1:6050004-6050023 MS.gene012268:CDS 45.0%
! CAATGGCCTTTTGGCCATTT+TGG - chr8.1:6049744-6049763 None:intergenic 45.0%
! GAAGACGTTTGAAATGGTGG+AGG + chr8.1:6048470-6048489 MS.gene012268:CDS 45.0%
! GAAGGTTGATTCAGACCGTT+GGG + chr8.1:6049442-6049461 MS.gene012268:CDS 45.0%
! GTTTGAAATGGTGGAGGATC+CGG + chr8.1:6048476-6048495 MS.gene012268:CDS 45.0%
!! GACTTACATAGGTGTTGAGC+TGG - chr8.1:6048626-6048645 None:intergenic 45.0%
ACCACCTGTTTCCACTCCTA+TGG + chr8.1:6049961-6049980 MS.gene012268:CDS 50.0%
AGTAAGCCAACAGATTGGGG+TGG + chr8.1:6050166-6050185 MS.gene012268:CDS 50.0%
CAGTCCATCTTCCATAGGAG+TGG - chr8.1:6049975-6049994 None:intergenic 50.0%
CTACGCAGTCCATCTTCCAT+AGG - chr8.1:6049980-6049999 None:intergenic 50.0%
GACCCGATTGTTTCATGGAG+TGG + chr8.1:6048504-6048523 MS.gene012268:CDS 50.0%
GGTGGTTACGACGGTGAAAT+CGG + chr8.1:6048177-6048196 MS.gene012268:CDS 50.0%
GTCTTCTTGAGAAACGGTGG+TGG - chr8.1:6048456-6048475 None:intergenic 50.0%
TCACATGCAGGGGTTTAGGA+AGG + chr8.1:6049424-6049443 MS.gene012268:intron 50.0%
TCCATAGGAGTGGAAACAGG+TGG - chr8.1:6049965-6049984 None:intergenic 50.0%
TCTGTCACATGCAGGGGTTT+AGG + chr8.1:6049420-6049439 MS.gene012268:intron 50.0%
TTCGGTTGAAACGTCGTCGT+AGG - chr8.1:6048277-6048296 None:intergenic 50.0%
! GGAAGGTTGATTCAGACCGT+TGG + chr8.1:6049441-6049460 MS.gene012268:CDS 50.0%
!! GCCTTTTGGCCATTTTGGTG+AGG - chr8.1:6049739-6049758 None:intergenic 50.0%
!! GGATCTGCTAGTGGATTGGT+GGG + chr8.1:6050187-6050206 MS.gene012268:CDS 50.0%
!! TGGATCTGCTAGTGGATTGG+TGG + chr8.1:6050186-6050205 MS.gene012268:CDS 50.0%
CATGACTTGAGGGCTTGTGG+TGG + chr8.1:6050007-6050026 MS.gene012268:CDS 55.0%
GATTGGGGTGGATCTGCTAG+TGG + chr8.1:6050178-6050197 MS.gene012268:CDS 55.0%
GCAGATCCACCCCAATCTGT+TGG - chr8.1:6050175-6050194 None:intergenic 55.0%
GGTTACGACGGTGAAATCGG+TGG + chr8.1:6048180-6048199 MS.gene012268:CDS 55.0%
! GGGTGGATCTGCTAGTGGAT+TGG + chr8.1:6050183-6050202 MS.gene012268:CDS 55.0%
GTGGCACACGGTGGTTACGA+CGG + chr8.1:6048168-6048187 MS.gene012268:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 6048162 6050222 6048162 ID=MS.gene012268
chr8.1 mRNA 6048162 6050222 6048162 ID=MS.gene012268.t1;Parent=MS.gene012268
chr8.1 exon 6048162 6048638 6048162 ID=MS.gene012268.t1.exon1;Parent=MS.gene012268.t1
chr8.1 CDS 6048162 6048638 6048162 ID=cds.MS.gene012268.t1;Parent=MS.gene012268.t1
chr8.1 exon 6049434 6050222 6049434 ID=MS.gene012268.t1.exon2;Parent=MS.gene012268.t1
chr8.1 CDS 6049434 6050222 6049434 ID=cds.MS.gene012268.t1;Parent=MS.gene012268.t1
Gene Sequence

>MS.gene012268

ATGGCGGTGGCACACGGTGGTTACGACGGTGAAATCGGTGGCTACAATTGTCGCCTAATCCCATCAATAAAACCAGAGTTATTCAACGACAGTGCAAATTCAAGCTCTTACACCTACGACGACGTTTCAACCGAAAGAAAAGAATCAGTACTAACCGAAGAAGAAGAAGAAGAAGGTGCTACAGCTTCTCACGCAATTCATATCAAAGAAGAGGTTCTTGAAGAAGACGGTGCTAACGCTTCATCTTCACTCAGGTTTCCTAAACCAATGGAAGGGTTACATGAGGTTGGTCCACCACCGTTTCTCAAGAAGACGTTTGAAATGGTGGAGGATCCGGAAACTGACCCGATTGTTTCATGGAGTGGCTCTCGTGATAGTTTTGTTGTTTGGGATTCTCACGAATTCTCCAAAATTCTTCTTCCTAAGTACTTTAAGCATAATAATTTTTCTAGCTTCATTCGCCAGCTCAACACCTATGTAAGTCAAAAAAATTCTCTATATCTTCATATGTTATCATTGCATACATATTTATAATCTTGTACAATTTAAATCAGTTTTGGCATCACGGTTTTGTTCAATCCACATGATACCAAACATGCATTGAGTTGATTTTCTAAAATTAATTTTGTTAAAAAGTTGATTCATACATAAAATGATTCATATTTATAGGGAGCTATGAATGTTTGGTTCCTCTTTTAGAGGAGATGTTGAATTGATTAAATAGATGTTGAATTGATTTTTGACATGTTTGTTGTTCTTGATTAGAATGATTTTGCTTTTGTAACTGATTTTGATTTTAAGTTGAAGCTAAAAATTTGATTTTTTAATTCTAAAATTTATTGTTCAACTTATTTTACATGAACTCACTTTTATAGGAATGCATTCAATCATTTTACATTAAATTCGAACTTCATTGGAATCATTTCAATCAAAATCAATTTTTGTGCCATGTAAAAAAACATGAAAAAACTGAAGACTTCAAGATATCTTTGCAATTCGCCATTTGAATTTATTCTGGATTTTTACACTCAAATTCATATCTAATTTTTACACTCACTTTTAACTACGAACAATTGAACCAAAATCTATTTTGTTTTTTGCAACCACAAAAATAGGTGGAGACTAAAAATCAAGAGGTAAAAGCTCTTAGATTTTGCTTGAGAACATTCAAACAACTCTAAATCAATTCTATATTTTTAAAATCACTTTGTATCTCTTAAAATTTAATACAAACATATTCTTTGTTGAATTTTATGAATCTGTCACATGCAGGGGTTTAGGAAGGTTGATTCAGACCGTTGGGAGTTTGCAAATGAAGGATTTCAAGCAGGGAAAAAGCATTTGTTGAAGAACATAAGAAGGAGAAGCAAGTATAACAAACTGCATCAAGGAGGATTCAATTTGATGAATCCTTGCGTTGAATCTGAAGTGGAGAAACTGAAAAAAGATCAGAACATGTTGAAATTGGAAATTCTGAAGCTAAGGCAACAACAAGAGAATTCAAATATTCAACTCACAAATGTTCAGGAGCGAGTTCAGCGAGCAGAATCGAAGCAATATCAGATGCTATTTTTCCTCACCAAAATGGCCAAAAGGCCATTGTTTATGGAGCAGATAATTCAAAAGATTAAGCGAAAAGGAGAAGTTGATGCTAATATAGACATGGTTAAAAGACCTAGATTGCTTGGAACACAAGGTACTAATGTTGATTATAGGAGTCAGGGTCGTGAACAATTTGCTACTTTGCAATCTGAATTGAATGGACTTTTTCCTGAAAATATGAACACTGGTAGAATGGAACCACCTGTTTCCACTCCTATGGAAGATGGACTGCGTAGCTCTTTACATGACTTGAGGGCTTGTGGTGGTTCTAGACAAAGTGTTCAAGATGCGACTTCTGCATATCATGTTATGTCAGAGAAGCTTTTGGGGGAAAATTCTGTTGTTGATGAAGAAATGGATGTGAATGACTCTAATATCTATCTTGAATTAGAGGATTTGATAAGTAAGCCAACAGATTGGGGTGGATCTGCTAGTGGATTGGTGGGACAAACTAGTTGA
Protein sequence

>MS.gene012268.t1

MAVAHGGYDGEIGGYNCRLIPSIKPELFNDSANSSSYTYDDVSTERKESVLTEEEEEEGATASHAIHIKEEVLEEDGANASSSLRFPKPMEGLHEVGPPPFLKKTFEMVEDPETDPIVSWSGSRDSFVVWDSHEFSKILLPKYFKHNNFSSFIRQLNTYGFRKVDSDRWEFANEGFQAGKKHLLKNIRRRSKYNKLHQGGFNLMNPCVESEVEKLKKDQNMLKLEILKLRQQQENSNIQLTNVQERVQRAESKQYQMLFFLTKMAKRPLFMEQIIQKIKRKGEVDANIDMVKRPRLLGTQGTNVDYRSQGREQFATLQSELNGLFPENMNTGRMEPPVSTPMEDGLRSSLHDLRACGGSRQSVQDATSAYHVMSEKLLGENSVVDEEMDVNDSNIYLELEDLISKPTDWGGSASGLVGQTS