Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012398.t1 | AES92327.2 | 97.1 | 547 | 16 | 0 | 1 | 547 | 93 | 639 | 0.00E+00 | 1097.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012398.t1 | Q9XI84 | 24.1 | 278 | 192 | 9 | 144 | 410 | 47 | 316 | 9.4e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012398.t1 | G7JVG8 | 97.1 | 547 | 16 | 0 | 1 | 547 | 93 | 639 | 0.0e+00 | 1097.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene012398.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012398.t1 | MTR_4g128240 | 97.075 | 547 | 16 | 0 | 1 | 547 | 93 | 639 | 0.0 | 1105 |
| MS.gene012398.t1 | MTR_3g082690 | 21.951 | 410 | 278 | 12 | 142 | 518 | 63 | 463 | 4.83e-18 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012398.t1 | AT2G18850 | 51.873 | 534 | 251 | 4 | 5 | 537 | 10 | 538 | 0.0 | 556 |
| MS.gene012398.t1 | AT2G18850 | 51.498 | 534 | 246 | 5 | 5 | 537 | 10 | 531 | 0.0 | 548 |
| MS.gene012398.t1 | AT3G07670 | 23.567 | 471 | 315 | 18 | 82 | 520 | 15 | 472 | 5.57e-21 | 96.7 |
| MS.gene012398.t1 | AT3G07670 | 24.127 | 315 | 205 | 11 | 234 | 520 | 26 | 334 | 1.51e-15 | 79.0 |
Find 116 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATTTAATTTCTGATTT+TGG | 0.059202 | 8.3:+2991577 | MS.gene012398:CDS |
| TGGTCTTCCCTCTCCATTAT+TGG | 0.251856 | 8.3:+2999601 | MS.gene012398:CDS |
| ATACATTATCTTCATGCTAT+TGG | 0.253277 | 8.3:-2998584 | None:intergenic |
| GTTTCAACTTCTTGCTGTAC+TGG | 0.267684 | 8.3:+3000577 | MS.gene012398:CDS |
| TAATTTGCAGGATTGAGTTT+TGG | 0.273161 | 8.3:+2995377 | MS.gene012398:intron |
| GTACTCCCTTCATATTGTTT+TGG | 0.278110 | 8.3:-2991603 | None:intergenic |
| CCGTATGATGTCATTCCTTT+AGG | 0.285448 | 8.3:+2999330 | MS.gene012398:CDS |
| GAAAATGAGCTAGAAGTTCT+TGG | 0.287372 | 8.3:+3000022 | MS.gene012398:CDS |
| CTGAGACAATATTGCTGTTA+TGG | 0.304721 | 8.3:+2992569 | MS.gene012398:CDS |
| GAGAGCCTCCAACTCATTCC+TGG | 0.355681 | 8.3:-2991467 | None:intergenic |
| TCAGAAATTACTACGATGTA+AGG | 0.359589 | 8.3:+2991023 | MS.gene012398:intron |
| AATCACAGCATATTCTATTA+TGG | 0.363717 | 8.3:+2999581 | MS.gene012398:CDS |
| TTCACAGTTGCAAGGAAACT+TGG | 0.371170 | 8.3:+3000000 | MS.gene012398:intron |
| ACATGTTTGATACCTCTTGC+TGG | 0.371762 | 8.3:+2998625 | MS.gene012398:CDS |
| AGATGTTGAAGGAGCAGGTC+GGG | 0.384155 | 8.3:+2991837 | MS.gene012398:intron |
| AGCACTCCTCAGGACCTTCT+TGG | 0.387075 | 8.3:+2991076 | MS.gene012398:CDS |
| TGACAACATGATGGACAGTA+TGG | 0.394682 | 8.3:+3000063 | MS.gene012398:CDS |
| ACCCTTGCAGGACGTTTGTA+TGG | 0.396858 | 8.3:-2991540 | None:intergenic |
| ATCTCTGGAGGAAATATATC+AGG | 0.406816 | 8.3:-2998517 | None:intergenic |
| CTCCATTATTGGATCATCTT+CGG | 0.411384 | 8.3:+2999612 | MS.gene012398:CDS |
| CTATCAAGACCATGCCGATC+TGG | 0.413805 | 8.3:+2999225 | MS.gene012398:CDS |
| ATAGAATATGCTGTGATTGT+TGG | 0.416388 | 8.3:-2999576 | None:intergenic |
| CACCATGTGAGTCGTCCATT+TGG | 0.419396 | 8.3:-2999537 | None:intergenic |
| TGCCCACATATTACACACTA+TGG | 0.422578 | 8.3:+2999165 | MS.gene012398:CDS |
| CTTGAGATGTATCCGTTCCT+TGG | 0.422866 | 8.3:-2991055 | None:intergenic |
| TTCTCCCAAGTGTAAATCTC+TGG | 0.423678 | 8.3:-2998532 | None:intergenic |
| CTCCGAAGATGATCCAATAA+TGG | 0.426430 | 8.3:-2999614 | None:intergenic |
| AATTTCAATGAATTTGTTGA+AGG | 0.427682 | 8.3:-2999198 | None:intergenic |
| GCTTGTGCTTGAAACTGATA+TGG | 0.430090 | 8.3:+2991936 | MS.gene012398:CDS |
| AAAGTCTTTGTTTGCATCAC+AGG | 0.436089 | 8.3:-2999647 | None:intergenic |
| GAAGCAATTACAATGCTAGA+TGG | 0.437043 | 8.3:+2995404 | MS.gene012398:CDS |
| TTACTACGATGTAAGGGGTT+TGG | 0.439522 | 8.3:+2991030 | MS.gene012398:CDS |
| AAGCTCCTCAGAAATGATAA+TGG | 0.442652 | 8.3:-2991917 | None:intergenic |
| TTTCAACTTCTTGCTGTACT+GGG | 0.444329 | 8.3:+3000578 | MS.gene012398:CDS |
| CTCACATGGTGCGTGGTACC+TGG | 0.452049 | 8.3:+2999549 | MS.gene012398:CDS |
| TCCTTTCTTTGATGAAAAGA+AGG | 0.455845 | 8.3:+2990589 | MS.gene012398:CDS |
| GAATGAACTGCACAAGTGTT+TGG | 0.459339 | 8.3:+3000615 | MS.gene012398:CDS |
| TGTTTGGCTGAGGACATTGT+AGG | 0.459596 | 8.3:+3000631 | MS.gene012398:CDS |
| TTGGTTCCTGCTTTGTGCAA+TGG | 0.460579 | 8.3:+2998490 | MS.gene012398:CDS |
| TTGAGATGTATCCGTTCCTT+GGG | 0.461230 | 8.3:-2991054 | None:intergenic |
| TTAATCTACACAGATGTTGA+AGG | 0.462913 | 8.3:+2991826 | MS.gene012398:intron |
| AGGATAAACCTGTGTCCAAA+TGG | 0.465266 | 8.3:+2999522 | MS.gene012398:CDS |
| GCAGGTCGGGGAATGATAGC+AGG | 0.465729 | 8.3:+2991850 | MS.gene012398:CDS |
| GTGAAGTCGATCTTGATAAC+AGG | 0.473430 | 8.3:+3000086 | MS.gene012398:CDS |
| TTCTGATGTTGATTCTATTG+AGG | 0.474846 | 8.3:+2999502 | MS.gene012398:CDS |
| TCAGTATCGTCTCTTGAAAG+AGG | 0.476597 | 8.3:+2992531 | MS.gene012398:intron |
| GAGAAGAACACCTGATTGAG+TGG | 0.477724 | 8.3:+2991650 | MS.gene012398:CDS |
| GCAGGGAAAGATCTAAAAGT+TGG | 0.483249 | 8.3:+2991868 | MS.gene012398:CDS |
| TGGGGACCAAAACAATATGA+AGG | 0.487327 | 8.3:+2991597 | MS.gene012398:CDS |
| GAAGAATGCTGCTTAAGCTA+TGG | 0.488333 | 8.3:+2999249 | MS.gene012398:CDS |
| CGTCTCTTGAAAGAGGTTGA+TGG | 0.489397 | 8.3:+2992538 | MS.gene012398:CDS |
| AGCAAGAAGTTGAAACAGAA+TGG | 0.489908 | 8.3:-3000570 | None:intergenic |
| CCTAAAGGAATGACATCATA+CGG | 0.505016 | 8.3:-2999330 | None:intergenic |
| GCTGTGATTGTTGGAGAGCC+AGG | 0.506463 | 8.3:-2999567 | None:intergenic |
| AAAAGCCATGGTGTGAAGAC+AGG | 0.508669 | 8.3:+2991676 | MS.gene012398:CDS |
| CAGCCCCAGGAATGAGTTGG+AGG | 0.515961 | 8.3:+2991462 | MS.gene012398:CDS |
| AGAGCCTCCAACTCATTCCT+GGG | 0.519139 | 8.3:-2991466 | None:intergenic |
| TCCTCCAGAGATTTACACTT+GGG | 0.521467 | 8.3:+2998528 | MS.gene012398:CDS |
| ACGATATCATTCATCCAAGA+AGG | 0.525319 | 8.3:-2991090 | None:intergenic |
| ATACATCTCAAGCACTCCTC+AGG | 0.526278 | 8.3:+2991066 | MS.gene012398:CDS |
| AAATGTAATAAAATGAGAGC+TGG | 0.529710 | 8.3:-2999280 | None:intergenic |
| TACACAGATGTTGAAGGAGC+AGG | 0.529975 | 8.3:+2991832 | MS.gene012398:intron |
| GGAAAACCATTGCACAAAGC+AGG | 0.530912 | 8.3:-2998496 | None:intergenic |
| CAGAAATTACTACGATGTAA+GGG | 0.531459 | 8.3:+2991024 | MS.gene012398:intron |
| CGTGTCCATTATCATTTCTG+AGG | 0.534034 | 8.3:+2991912 | MS.gene012398:CDS |
| TTCCATACAAACGTCCTGCA+AGG | 0.535585 | 8.3:+2991538 | MS.gene012398:CDS |
| ATGTAAGGGGTTTGGTCCCA+AGG | 0.536177 | 8.3:+2991038 | MS.gene012398:CDS |
| AAGAATGCTGCTTAAGCTAT+GGG | 0.536764 | 8.3:+2999250 | MS.gene012398:CDS |
| TTCTCAGAGAAAGCTACACC+TGG | 0.537382 | 8.3:+3000500 | MS.gene012398:intron |
| GCAAGAAGTTGAAACAGAAT+GGG | 0.537385 | 8.3:-3000569 | None:intergenic |
| TTACAGCCCCAGGAATGAGT+TGG | 0.537964 | 8.3:+2991459 | MS.gene012398:CDS |
| AGCAGCATTCTTCTCCAGAT+CGG | 0.542200 | 8.3:-2999239 | None:intergenic |
| GCACTCCTCGTCACTATCCA+TGG | 0.550146 | 8.3:-2990537 | None:intergenic |
| AGAGATAATGCAATCAAGAC+AGG | 0.553032 | 8.3:+2995442 | MS.gene012398:CDS |
| TGAGTCGTCCATTTGGACAC+AGG | 0.564379 | 8.3:-2999530 | None:intergenic |
| CATTATCTCTTCAAACAGCA+AGG | 0.565535 | 8.3:-2995429 | None:intergenic |
| CTCAGAAATGATAATGGACA+CGG | 0.571725 | 8.3:-2991911 | None:intergenic |
| GATGTTGAAGGAGCAGGTCG+GGG | 0.571834 | 8.3:+2991838 | MS.gene012398:intron |
| TCCATACAAACGTCCTGCAA+GGG | 0.573730 | 8.3:+2991539 | MS.gene012398:CDS |
| GCATGCTCAGTATGATGAGT+TGG | 0.574500 | 8.3:+2998471 | MS.gene012398:CDS |
| AGATGATCCAATAATGGAGA+GGG | 0.579934 | 8.3:-2999608 | None:intergenic |
| TTCCTCCAGAGATTTACACT+TGG | 0.582164 | 8.3:+2998527 | MS.gene012398:CDS |
| TTGCCATAGTGTGTAATATG+TGG | 0.582672 | 8.3:-2999168 | None:intergenic |
| AGAAGAACACCTGATTGAGT+GGG | 0.587488 | 8.3:+2991651 | MS.gene012398:CDS |
| AGAAATTACTACGATGTAAG+GGG | 0.589340 | 8.3:+2991025 | MS.gene012398:intron |
| GGGGACCAAAACAATATGAA+GGG | 0.590986 | 8.3:+2991598 | MS.gene012398:CDS |
| AGACATCTTTGACAACATGA+TGG | 0.594876 | 8.3:+3000054 | MS.gene012398:CDS |
| TTAATCCTGTCTTCACACCA+TGG | 0.598864 | 8.3:-2991681 | None:intergenic |
| AGGGGTTTGGTCCCAAGGAA+CGG | 0.599513 | 8.3:+2991043 | MS.gene012398:CDS |
| ATGAAGATAATGTATTCAGA+TGG | 0.601114 | 8.3:+2998592 | MS.gene012398:CDS |
| CAGATGTTGAAGGAGCAGGT+CGG | 0.610233 | 8.3:+2991836 | MS.gene012398:intron |
| TGATTAAGAAAGCCAGCAAG+AGG | 0.615019 | 8.3:-2998637 | None:intergenic |
| TCCCAAGTGTAAATCTCTGG+AGG | 0.620554 | 8.3:-2998529 | None:intergenic |
| CATTCTTCTCCAGATCGGCA+TGG | 0.630402 | 8.3:-2999234 | None:intergenic |
| GAGCCTCCAACTCATTCCTG+GGG | 0.630829 | 8.3:-2991465 | None:intergenic |
| GTTCTTCTCTCTCGCAACTG+TGG | 0.638819 | 8.3:-2991636 | None:intergenic |
| TGCTGAAGACGATGCCTCGG+TGG | 0.645814 | 8.3:+2991432 | MS.gene012398:CDS |
| TGGACGACTCACATGGTGCG+TGG | 0.646565 | 8.3:+2999542 | MS.gene012398:CDS |
| CTAAAGGAATGACATCATAC+GGG | 0.653617 | 8.3:-2999329 | None:intergenic |
| CAGGTCGGGGAATGATAGCA+GGG | 0.654752 | 8.3:+2991851 | MS.gene012398:CDS |
| TCAGAAATGATAATGGACAC+GGG | 0.657279 | 8.3:-2991910 | None:intergenic |
| TGCCATAGTGTGTAATATGT+GGG | 0.660167 | 8.3:-2999167 | None:intergenic |
| AAGATGATCCAATAATGGAG+AGG | 0.665042 | 8.3:-2999609 | None:intergenic |
| AGGACGTTTGTATGGAAATG+TGG | 0.671538 | 8.3:-2991532 | None:intergenic |
| TGCAGCCATGGATAGTGACG+AGG | 0.679797 | 8.3:+2990532 | None:intergenic |
| GTCCAAATGGACGACTCACA+TGG | 0.681562 | 8.3:+2999535 | MS.gene012398:CDS |
| TCTCAGAGAAAGCTACACCT+GGG | 0.684262 | 8.3:+3000501 | MS.gene012398:intron |
| TCATACGGGTTGTCTCCTTG+AGG | 0.685652 | 8.3:-2999315 | None:intergenic |
| ATTCATCCAAGAAGGTCCTG+AGG | 0.686111 | 8.3:-2991082 | None:intergenic |
| ATTGAGTGGGGTAAAAGCCA+TGG | 0.686263 | 8.3:+2991664 | MS.gene012398:CDS |
| GACAACATGATGGACAGTAT+GGG | 0.688012 | 8.3:+3000064 | MS.gene012398:CDS |
| GAAGAACACCTGATTGAGTG+GGG | 0.693168 | 8.3:+2991652 | MS.gene012398:CDS |
| ACTGCACAAGTGTTTGGCTG+AGG | 0.693657 | 8.3:+3000621 | MS.gene012398:CDS |
| TGTATTCAGATGGAAAACTG+AGG | 0.723155 | 8.3:+2998602 | MS.gene012398:CDS |
| AAGAATCATACAGTTAAACG+AGG | 0.733021 | 8.3:+2991134 | MS.gene012398:CDS |
| GGGGCTGTAAAACTCCACCG+AGG | 0.821008 | 8.3:-2991446 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 2990539 | 3000657 | 2990539 | ID=MS.gene012398 |
| chr8.3 | mRNA | 2990539 | 3000657 | 2990539 | ID=MS.gene012398.t1;Parent=MS.gene012398 |
| chr8.3 | exon | 2990539 | 2990610 | 2990539 | ID=MS.gene012398.t1.exon1;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2990539 | 2990610 | 2990539 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2991027 | 2991155 | 2991027 | ID=MS.gene012398.t1.exon2;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2991027 | 2991155 | 2991027 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2991418 | 2991712 | 2991418 | ID=MS.gene012398.t1.exon3;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2991418 | 2991712 | 2991418 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2991839 | 2991957 | 2991839 | ID=MS.gene012398.t1.exon4;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2991839 | 2991957 | 2991839 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2992535 | 2992667 | 2992535 | ID=MS.gene012398.t1.exon5;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2992535 | 2992667 | 2992535 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2995387 | 2995463 | 2995387 | ID=MS.gene012398.t1.exon6;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2995387 | 2995463 | 2995387 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2998466 | 2998663 | 2998466 | ID=MS.gene012398.t1.exon7;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2998466 | 2998663 | 2998466 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2999162 | 2999351 | 2999162 | ID=MS.gene012398.t1.exon8;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2999162 | 2999351 | 2999162 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 2999492 | 2999670 | 2999492 | ID=MS.gene012398.t1.exon9;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 2999492 | 2999670 | 2999492 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 3000007 | 3000107 | 3000007 | ID=MS.gene012398.t1.exon10;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 3000007 | 3000107 | 3000007 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| chr8.3 | exon | 3000507 | 3000657 | 3000507 | ID=MS.gene012398.t1.exon11;Parent=MS.gene012398.t1 |
| chr8.3 | CDS | 3000507 | 3000657 | 3000507 | ID=cds.MS.gene012398.t1;Parent=MS.gene012398.t1 |
| Gene Sequence |
| Protein sequence |
