AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012599

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012599.t1 MTR_4g131580 94.518 602 32 1 1 601 1 602 0.0 1126
MS.gene012599.t1 MTR_4g131570 77.066 593 126 5 1 587 1 589 0.0 883
MS.gene012599.t1 MTR_4g131600 71.695 590 158 4 1 589 4 585 0.0 815
MS.gene012599.t1 MTR_2g450070 42.773 678 295 21 3 602 4 666 4.47e-128 392
MS.gene012599.t1 MTR_2g450070 42.688 677 295 21 3 601 4 665 4.86e-127 390
MS.gene012599.t1 MTR_3g106220 44.118 408 191 8 17 402 8 400 8.52e-93 299
MS.gene012599.t1 MTR_1g013170 47.135 384 176 10 17 382 5 379 1.35e-91 291
MS.gene012599.t1 MTR_1g032570 48.464 293 138 6 17 297 11 302 3.07e-83 273
MS.gene012599.t1 MTR_4g121020 42.559 383 184 7 17 375 31 401 1.08e-81 271
MS.gene012599.t1 MTR_4g121020 42.559 383 184 7 17 375 31 401 1.38e-81 270
MS.gene012599.t1 MTR_1g013180 49.186 307 146 3 17 314 156 461 1.47e-79 265
MS.gene012599.t1 MTR_2g034960 46.687 332 158 7 9 326 21 347 2.32e-79 263
MS.gene012599.t1 MTR_7g117705 49.823 283 135 5 15 292 3 283 2.86e-78 256
MS.gene012599.t1 MTR_8g079940 44.311 334 147 9 15 327 23 338 4.71e-77 254
MS.gene012599.t1 MTR_3g086100 51.908 262 93 4 17 260 13 259 5.06e-75 250
MS.gene012599.t1 MTR_1g032570 47.863 234 109 6 76 297 1 233 1.41e-58 206
MS.gene012599.t1 MTR_3g102590 47.541 244 78 2 11 253 2 196 4.13e-58 195
MS.gene012599.t1 MTR_3g102590 46.667 240 78 2 11 249 2 192 1.30e-54 184
MS.gene012599.t1 MTR_3g086100 50.246 203 68 4 76 260 1 188 6.17e-50 181
MS.gene012599.t1 MTR_3g102600 63.710 124 45 0 17 140 8 131 3.81e-47 164
MS.gene012599.t1 MTR_3g102600 59.677 62 24 1 191 251 122 183 1.23e-16 79.0
MS.gene012599.t1 MTR_3g102600 63.710 124 45 0 17 140 8 131 9.77e-47 164
MS.gene012599.t1 MTR_3g102600 64.706 68 23 1 191 257 122 189 2.85e-20 90.5
MS.gene012599.t1 MTR_4g021760 34.274 248 138 3 16 263 10 232 9.53e-44 159
MS.gene012599.t1 MTR_4g021790 36.437 247 132 4 17 263 10 231 1.90e-43 157
MS.gene012599.t1 MTR_4g121020 36.842 304 156 7 96 375 6 297 9.73e-43 162
MS.gene012599.t1 MTR_0450s0040 36.154 260 133 5 21 279 8 235 8.22e-42 154
MS.gene012599.t1 MTR_4g021855 36.032 247 133 5 17 263 10 231 3.80e-41 151
MS.gene012599.t1 MTR_4g021845 37.021 235 121 6 16 249 9 217 5.80e-40 145
MS.gene012599.t1 MTR_6g045327 33.600 250 139 3 15 263 12 235 1.48e-39 149
MS.gene012599.t1 MTR_1g013160 50.000 148 71 2 79 223 1 148 8.87e-37 135
MS.gene012599.t1 MTR_3g102600 64.634 82 29 0 59 140 1 82 3.96e-31 120
MS.gene012599.t1 MTR_3g102600 64.706 68 23 1 191 257 73 140 1.51e-20 89.7
MS.gene012599.t1 MTR_3g102600 64.634 82 29 0 59 140 1 82 4.36e-31 118
MS.gene012599.t1 MTR_3g102600 61.017 59 22 1 194 251 76 134 2.47e-16 76.6
MS.gene012599.t1 MTR_8g105600 34.298 242 152 3 17 257 9 244 5.73e-31 128
MS.gene012599.t1 MTR_7g026400 34.685 222 128 3 47 266 17 223 2.43e-29 123
MS.gene012599.t1 MTR_5g055260 32.365 241 111 6 17 257 10 198 6.23e-29 116
MS.gene012599.t1 MTR_8g032710 25.769 260 172 4 16 255 16 274 4.64e-24 106
MS.gene012599.t1 MTR_3g092780 37.795 127 74 2 21 143 56 181 1.24e-23 105
MS.gene012599.t1 MTR_3g092780 37.795 127 74 2 21 143 56 181 1.50e-23 105
MS.gene012599.t1 MTR_3g092780 37.903 124 72 2 21 140 56 178 4.53e-23 104
MS.gene012599.t1 MTR_3g092780 37.903 124 72 2 21 140 56 178 5.64e-23 103
MS.gene012599.t1 MTR_7g118260 36.090 133 76 2 18 144 11 140 1.98e-22 102
MS.gene012599.t1 MTR_7g118260 36.719 128 72 2 18 139 11 135 4.38e-22 100
MS.gene012599.t1 MTR_4g023980 33.540 161 83 4 63 223 35 171 4.79e-22 94.0
MS.gene012599.t1 MTR_4g061360 39.231 130 75 2 14 139 81 210 5.72e-22 101
MS.gene012599.t1 MTR_8g024260 36.000 125 77 1 23 144 38 162 4.14e-21 98.2
MS.gene012599.t1 MTR_1g067110 38.525 122 72 1 21 139 85 206 7.03e-21 97.4
MS.gene012599.t1 MTR_1g067110 38.525 122 72 1 21 139 85 206 7.73e-21 97.4
MS.gene012599.t1 MTR_4g064730 61.842 76 26 1 182 257 116 188 1.12e-20 93.6
MS.gene012599.t1 MTR_8g093040 28.049 246 135 6 17 261 14 218 1.90e-20 92.0
MS.gene012599.t1 MTR_6g016850 32.836 134 87 2 17 148 9 141 1.07e-18 84.3
MS.gene012599.t1 MTR_7g089010 53.846 78 36 0 180 257 71 148 1.21e-18 87.4
MS.gene012599.t1 MTR_3g037390 33.333 123 79 1 21 140 24 146 7.09e-18 87.8
MS.gene012599.t1 MTR_4g108880 33.333 123 79 1 21 140 33 155 7.47e-18 87.8
MS.gene012599.t1 MTR_5g014040 39.516 124 71 2 17 139 14 134 4.67e-16 82.0
MS.gene012599.t1 MTR_5g014040 59.016 61 25 0 193 253 304 364 9.32e-15 77.8
MS.gene012599.t1 MTR_5g014040 39.516 124 71 2 17 139 14 134 5.03e-16 81.6
MS.gene012599.t1 MTR_5g014040 54.545 66 25 1 193 253 304 369 8.22e-13 71.6
MS.gene012599.t1 MTR_4g098870 62.069 58 22 0 196 253 306 363 6.24e-15 78.2
MS.gene012599.t1 MTR_4g098870 36.290 124 75 2 17 139 14 134 1.37e-14 77.4
MS.gene012599.t1 MTR_4g098870 62.069 58 22 0 196 253 306 363 6.71e-15 78.2
MS.gene012599.t1 MTR_4g098870 36.290 124 75 2 17 139 14 134 1.95e-14 76.6
MS.gene012599.t1 MTR_4g098870 62.069 58 22 0 196 253 326 383 6.80e-15 78.2
MS.gene012599.t1 MTR_4g098870 36.290 124 75 2 17 139 34 154 1.81e-14 77.0
MS.gene012599.t1 MTR_4g098870 62.069 58 22 0 196 253 345 402 7.12e-15 78.2
MS.gene012599.t1 MTR_4g098870 36.290 124 75 2 17 139 53 173 1.88e-14 77.0
MS.gene012599.t1 MTR_4g098870 62.069 58 22 0 196 253 326 383 7.43e-15 78.2
MS.gene012599.t1 MTR_4g098870 36.290 124 75 2 17 139 34 154 2.50e-14 76.6
MS.gene012599.t1 MTR_1g093080 51.471 68 32 1 193 259 204 271 1.99e-14 75.9
MS.gene012599.t1 MTR_4g113140 46.429 84 41 2 197 279 232 312 4.70e-14 75.1
MS.gene012599.t1 MTR_5g014040 54.545 66 25 1 193 253 264 329 7.28e-13 71.6
MS.gene012599.t1 MTR_4g086835 52.459 61 29 0 197 257 271 331 7.85e-13 71.2
MS.gene012599.t1 MTR_7g490310 30.612 147 79 3 23 148 11 155 1.37e-12 68.2
MS.gene012599.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 2.07e-12 68.9
MS.gene012599.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 3.26e-12 68.6
MS.gene012599.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 4.58e-12 68.2
MS.gene012599.t1 MTR_4g078810 40.476 84 48 1 198 281 156 237 4.79e-12 68.6
MS.gene012599.t1 MTR_7g115530 56.667 60 25 1 200 258 218 277 5.53e-12 68.6
MS.gene012599.t1 MTR_7g098250 46.835 79 40 2 196 272 258 336 6.31e-12 68.6
MS.gene012599.t1 MTR_5g017980 50.847 59 29 0 199 257 192 250 6.87e-12 67.4
MS.gene012599.t1 MTR_5g017980 50.847 59 29 0 199 257 191 249 7.01e-12 67.4
MS.gene012599.t1 MTR_2g090675 41.333 75 43 1 178 252 140 213 7.80e-12 68.2
MS.gene012599.t1 MTR_1g080330 51.562 64 30 1 196 258 171 234 7.85e-12 66.6
MS.gene012599.t1 MTR_1g080330 51.562 64 30 1 196 258 171 234 9.82e-12 67.4
MS.gene012599.t1 MTR_6g088950 29.545 132 85 2 47 175 34 160 2.10e-11 64.3
MS.gene012599.t1 MTR_4g086835 51.613 62 29 1 197 257 271 332 2.33e-11 66.6
MS.gene012599.t1 MTR_1874s0010 31.858 113 73 2 19 129 2 112 2.65e-11 64.3
MS.gene012599.t1 MTR_5g036480 26.163 172 107 4 21 172 16 187 2.72e-11 64.3
MS.gene012599.t1 MTR_5g054300 49.180 61 30 1 198 257 196 256 4.02e-11 65.5
MS.gene012599.t1 MTR_2g086450 53.333 60 27 1 196 254 41 100 6.51e-11 65.1
MS.gene012599.t1 MTR_5g041350 52.542 59 27 1 196 253 11 69 6.99e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012599.t1 AT2G25180 48.205 390 175 10 1 374 1 379 1.35e-94 302
MS.gene012599.t1 AT4G31920 51.562 320 131 6 17 325 13 319 1.65e-90 290
MS.gene012599.t1 AT5G58080 41.099 455 238 10 9 450 6 443 5.14e-82 270
MS.gene012599.t1 AT3G16857 47.987 298 128 3 15 288 31 325 6.84e-80 266
MS.gene012599.t1 AT3G16857 47.987 298 128 3 15 288 31 325 1.24e-79 265
MS.gene012599.t1 AT1G67710 55.253 257 96 5 16 260 5 254 5.29e-77 254
MS.gene012599.t1 AT2G01760 49.810 263 108 5 17 262 7 262 1.49e-66 222
MS.gene012599.t1 AT3G16857 47.471 257 108 3 56 288 2 255 8.99e-66 227
MS.gene012599.t1 AT2G01760 45.017 291 108 6 17 262 7 290 1.99e-60 207
MS.gene012599.t1 AT1G67710 53.571 196 73 4 76 260 1 189 4.17e-51 183
MS.gene012599.t1 AT5G49240 40.816 245 135 3 11 249 32 272 1.16e-50 177
MS.gene012599.t1 AT2G01760 50.000 204 78 5 76 262 1 197 2.61e-45 163
MS.gene012599.t1 AT3G62670 36.700 297 164 9 11 300 31 310 1.00e-39 151
MS.gene012599.t1 AT1G49190 29.967 307 168 7 17 300 30 312 1.81e-38 147
MS.gene012599.t1 AT1G67710 49.143 175 71 4 97 260 13 180 6.71e-37 143
MS.gene012599.t1 AT5G07210 29.710 276 153 4 21 262 16 284 2.25e-29 123
MS.gene012599.t1 AT5G07210 28.873 284 153 5 21 262 16 292 5.25e-27 116
MS.gene012599.t1 AT2G27070 30.182 275 158 6 21 263 16 288 5.32e-25 110
MS.gene012599.t1 AT5G24470 38.095 126 73 2 19 140 48 172 3.95e-23 103
MS.gene012599.t1 AT2G46790 36.806 144 83 4 3 139 16 158 4.42e-23 102
MS.gene012599.t1 AT2G46790 36.806 144 83 4 3 139 16 158 4.87e-23 103
MS.gene012599.t1 AT5G59570 61.250 80 30 1 178 257 120 198 1.09e-22 99.4
MS.gene012599.t1 AT5G59570 61.250 80 30 1 178 257 120 198 1.09e-22 99.4
MS.gene012599.t1 AT1G49190 36.800 125 79 0 17 141 30 154 1.13e-22 102
MS.gene012599.t1 AT5G60100 39.231 130 75 2 14 139 56 185 5.60e-22 99.4
MS.gene012599.t1 AT5G60100 39.231 130 75 2 14 139 56 185 5.60e-22 99.4
MS.gene012599.t1 AT5G60100 39.231 130 75 2 14 139 56 185 5.60e-22 99.4
MS.gene012599.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.23e-21 99.0
MS.gene012599.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.23e-21 99.0
MS.gene012599.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.23e-21 99.0
MS.gene012599.t1 AT5G60100 39.231 130 75 2 14 139 56 185 1.52e-21 99.0
MS.gene012599.t1 AT3G46640 62.667 75 27 1 183 257 128 201 2.32e-21 95.9
MS.gene012599.t1 AT3G46640 62.667 75 27 1 183 257 128 201 2.32e-21 95.9
MS.gene012599.t1 AT3G46640 62.667 75 27 1 183 257 128 201 2.74e-21 95.9
MS.gene012599.t1 AT5G05090 59.459 74 30 0 184 257 65 138 2.02e-20 92.0
MS.gene012599.t1 AT3G10760 63.636 66 24 0 192 257 97 162 1.65e-19 90.5
MS.gene012599.t1 AT5G02810 36.885 122 74 1 21 139 78 199 2.35e-19 92.8
MS.gene012599.t1 AT2G40970 63.077 65 24 0 193 257 98 162 5.24e-19 87.4
MS.gene012599.t1 AT1G68210 28.148 270 162 6 21 259 13 281 1.56e-18 90.1
MS.gene012599.t1 AT5G61380 34.959 123 77 1 21 140 19 141 1.19e-17 87.0
MS.gene012599.t1 AT5G44190 58.333 60 25 0 193 252 140 199 3.00e-15 78.6
MS.gene012599.t1 AT5G44190 58.333 60 25 0 193 252 140 199 3.44e-15 77.8
MS.gene012599.t1 AT1G25550 44.828 87 46 2 189 274 199 284 3.75e-14 74.7
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.85e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.64e-11 65.9
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 5.43e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.33e-11 66.2
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 5.43e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.33e-11 66.2
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 5.43e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.33e-11 66.2
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 5.43e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.33e-11 66.2
MS.gene012599.t1 AT4G18020 37.063 143 81 3 15 150 12 152 5.43e-14 75.5
MS.gene012599.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.33e-11 66.2
MS.gene012599.t1 AT2G03500 45.205 73 39 1 199 270 232 304 2.59e-13 72.8
MS.gene012599.t1 AT2G20570 48.101 79 38 1 174 252 130 205 2.86e-13 72.4
MS.gene012599.t1 AT1G68670 44.304 79 42 2 182 259 198 275 3.14e-13 72.0
MS.gene012599.t1 AT4G37180 43.373 83 46 1 176 257 193 275 1.87e-12 69.7
MS.gene012599.t1 AT4G37180 43.373 83 46 1 176 257 186 268 1.89e-12 69.7
MS.gene012599.t1 AT3G13040 51.562 64 30 1 199 261 240 303 1.06e-11 67.8
MS.gene012599.t1 AT3G13040 51.562 64 30 1 199 261 240 303 1.06e-11 67.8
MS.gene012599.t1 AT3G13040 51.562 64 30 1 199 261 240 303 1.06e-11 67.8
MS.gene012599.t1 AT2G20570 62.500 48 18 0 205 252 174 221 1.09e-11 67.8
MS.gene012599.t1 AT3G25790 51.724 58 27 1 204 260 200 257 1.74e-11 66.6
MS.gene012599.t1 AT1G79430 39.130 115 49 4 196 308 30 125 3.78e-11 65.5
MS.gene012599.t1 AT1G49560 45.070 71 38 1 199 268 191 261 3.81e-11 65.5
MS.gene012599.t1 AT3G12730 45.238 84 43 3 179 261 3 84 4.38e-11 63.9
MS.gene012599.t1 AT3G04030 53.333 60 27 1 196 254 41 100 4.68e-11 65.5
MS.gene012599.t1 AT3G04030 53.333 60 27 1 196 254 41 100 5.07e-11 65.5

Find 109 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATGATCAAATTTC+TGG 0.121819 8.1:+2273303 MS.gene012599:CDS
AGCTTGAAGCCATCCATTTC+TGG 0.175471 8.1:-2271646 None:intergenic
GTAGCAGGATTAAAAGATTT+TGG 0.205735 8.1:-2273231 None:intergenic
CCTCTCCCAGTTCTCACTTT+AGG 0.237398 8.1:+2273343 MS.gene012599:CDS
GTAAATCACTCTTCTGTTAG+AGG 0.250033 8.1:+2273195 MS.gene012599:CDS
AAACATCGGCTGCTTGAAAC+TGG 0.273444 8.1:-2272941 None:intergenic
TCCGGTTGGTATGCGTGTTC+TGG 0.308828 8.1:+2270779 MS.gene012599:CDS
TGAGGCATCTGAGTCGTTAA+TGG 0.327980 8.1:-2273579 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.338432 8.1:+2272757 MS.gene012599:CDS
TCGTTAATGGACTATTAGTT+TGG 0.342387 8.1:-2273566 None:intergenic
TTCGACACTTGCTGTTCCTT+CGG 0.359113 8.1:+2273386 MS.gene012599:CDS
CGTTAATGGACTATTAGTTT+GGG 0.359733 8.1:-2273565 None:intergenic
GAGCAACTGCTTGCTCTAGA+TGG 0.366890 8.1:+2273660 MS.gene012599:CDS
CTTCTCACATGCAAGTATAA+TGG 0.379037 8.1:+2270846 MS.gene012599:CDS
CACCTATCAACACATAATTC+AGG 0.390374 8.1:+2273165 MS.gene012599:CDS
TGAGCAAGTTGGACTTGAAA+TGG 0.392446 8.1:+2271672 MS.gene012599:CDS
GATAAATGTTCCGCTGAAAC+TGG 0.395287 8.1:-2273020 None:intergenic
AATAAGTGAAGCGGTTAAAC+AGG 0.412727 8.1:+2273719 MS.gene012599:CDS
AAAGCACTTGTATAATCAAT+TGG 0.414899 8.1:-2273066 None:intergenic
CTTAAGATTCTTCGAGATCC+AGG 0.425401 8.1:-2272796 None:intergenic
GTTTCTATAGTTAATCTGCT+TGG 0.431269 8.1:+2272173 MS.gene012599:CDS
CTTACTTGACAATGTTGTAA+AGG 0.437919 8.1:+2273434 MS.gene012599:CDS
AGTGAAGATGTTACCGAAGA+AGG 0.438022 8.1:+2270753 MS.gene012599:CDS
CCACGGATGTTCGAACGCCC+TGG 0.438585 8.1:-2272990 None:intergenic
ACTGGATCCTCTCCAGCAAA+AGG 0.442603 8.1:+2272738 MS.gene012599:CDS
ATCACTCAATGGAGAAGGAT+TGG 0.449366 8.1:-2273277 None:intergenic
TCTCAAACGACCGTCTAAAC+AGG 0.454349 8.1:+2272699 MS.gene012599:CDS
GCCAGAACACGCATACCAAC+CGG 0.455924 8.1:-2270780 None:intergenic
TAACAGTATCTCTGATGTCT+TGG 0.462540 8.1:-2273107 None:intergenic
ACCAACCGGAAAACCTTCTT+CGG 0.468377 8.1:-2270766 None:intergenic
TTCTGTTAGAGGTGCTTATG+TGG 0.470943 8.1:+2273206 MS.gene012599:CDS
TGAGAATAGTTGTGTGTCCT+TGG 0.476712 8.1:+2273689 MS.gene012599:CDS
TGATGTAAACATGCCAGAAA+TGG 0.478571 8.1:+2271633 MS.gene012599:CDS
CTTAAATTCATGTTACTAAG+TGG 0.478822 8.1:-2273042 None:intergenic
TTCTTCCTTCAACAATACCT+TGG 0.480661 8.1:+2273491 MS.gene012599:CDS
GATGGCTGTGGAGGATCGTT+TGG 0.483919 8.1:+2270728 None:intergenic
TTAGATGAACCATTTGTAGC+AGG 0.485124 8.1:-2273246 None:intergenic
TTGGTGTGATATAGTGTTGT+CGG 0.488347 8.1:+2271812 MS.gene012599:intron
GAGAAAGAAGATTGAAAGCA+AGG 0.491146 8.1:+2271944 MS.gene012599:CDS
CACGGATGTTCGAACGCCCT+GGG 0.491277 8.1:-2272989 None:intergenic
TGGTGTGATATAGTGTTGTC+GGG 0.492589 8.1:+2271813 MS.gene012599:intron
TGGAGGAGCTTAAGAACATA+TGG 0.492597 8.1:+2271910 MS.gene012599:CDS
GGCTAAAGTAGATGCTGCAC+TGG 0.493832 8.1:+2272720 MS.gene012599:CDS
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.1:+2272390 MS.gene012599:CDS
TTATAATTTGTACCTGCAGA+TGG 0.497679 8.1:-2272427 None:intergenic
ATCAAGGTATCGTTAGTGAT+GGG 0.497943 8.1:+2271973 MS.gene012599:CDS
TTAGCTTACTAATGAAAGGC+AGG 0.500886 8.1:-2271696 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.503061 8.1:-2273597 None:intergenic
TACTACAACTACGAATTCAA+TGG 0.503272 8.1:+2271558 MS.gene012599:CDS
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8.1:-2273496 None:intergenic
CGGGACATGATGATAGAGAA+AGG 0.508606 8.1:+2271832 MS.gene012599:CDS
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.1:+2271957 MS.gene012599:CDS
TGAGATTGAAGTGGAGGGAC+TGG 0.515637 8.1:-2272910 None:intergenic
TCAGACTAGTCTCAAAGTGC+TGG 0.521547 8.1:+2270815 MS.gene012599:CDS
TGGTTCCATCACTCTTGCAT+TGG 0.525055 8.1:-2273619 None:intergenic
CATTGCATCGCTTCCTCCGA+AGG 0.526768 8.1:-2273402 None:intergenic
TTTGTTAGCTTACTAATGAA+AGG 0.526782 8.1:-2271700 None:intergenic
AATCAAGGTATCGTTAGTGA+TGG 0.527040 8.1:+2271972 MS.gene012599:CDS
TGTTCTTAAGCTCCTCCAAT+CGG 0.529926 8.1:-2271905 None:intergenic
TTATTCCAATGCAAGAGTGA+TGG 0.531742 8.1:+2273614 MS.gene012599:CDS
CGTGACTTGATGAATGTTGA+AGG 0.537018 8.1:+2272380 MS.gene012599:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.537224 8.1:+2272751 MS.gene012599:CDS
TGGAAGCGCTTGAATTGTTG+AGG 0.538411 8.1:+2271578 MS.gene012599:CDS
ATTCTGGTTGATGATGGCTG+TGG 0.540952 8.1:+2270716 None:intergenic
CTGGATCCTCTCCAGCAAAA+GGG 0.541917 8.1:+2272739 MS.gene012599:CDS
CTCCTGAATTATGTGTTGAT+AGG 0.542919 8.1:-2273167 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.547513 8.1:+2271661 MS.gene012599:CDS
GGAAGCGCTTGAATTGTTGA+GGG 0.548067 8.1:+2271579 MS.gene012599:CDS
GACACTTGCTGTTCCTTCGG+AGG 0.551078 8.1:+2273389 MS.gene012599:CDS
AAATGTAGCTAGCCATCTGC+AGG 0.551114 8.1:+2272415 MS.gene012599:CDS
TTGTTAAAATGATCACTCAA+TGG 0.551340 8.1:-2273288 None:intergenic
AACATCGGCTGCTTGAAACT+GGG 0.553633 8.1:-2272940 None:intergenic
ATCGTTAGTGATGGGGTATG+TGG 0.558412 8.1:+2271981 MS.gene012599:CDS
CTGGTTGATGATGGCTGTGG+AGG 0.558423 8.1:+2270719 None:intergenic
TCTAGTCAAACCGATCCGAT+TGG 0.560044 8.1:+2271890 MS.gene012599:CDS
TTAAGCTCCTCCAATCGGAT+CGG 0.560387 8.1:-2271900 None:intergenic
TTTCAAGTCCAACTTGCTCA+AGG 0.564395 8.1:-2271669 None:intergenic
CTGAAACTGGTTAATTTCCA+CGG 0.564461 8.1:-2273007 None:intergenic
TCTACTTTAGCCTGTTTAGA+CGG 0.566286 8.1:-2272709 None:intergenic
TTCACTTATTGTGTCATCCA+AGG 0.566691 8.1:-2273706 None:intergenic
AAGAAAGGAACAAAGTGAGG+AGG 0.573387 8.1:+2272058 MS.gene012599:CDS
ATAAGAAGCAAGAGAATCCA+AGG 0.574630 8.1:-2273508 None:intergenic
AAATGATCACTCAATGGAGA+AGG 0.574716 8.1:-2273282 None:intergenic
TAATGAAGACACTAGTTCCA+TGG 0.575646 8.1:+2273530 MS.gene012599:CDS
AGTCAAACCGATCCGATTGG+AGG 0.576963 8.1:+2271893 MS.gene012599:CDS
CCAGGGCGTTCGAACATCCG+TGG 0.583592 8.1:+2272990 MS.gene012599:CDS
GTCATATAAACTCACTGTCA+AGG 0.583894 8.1:-2272196 None:intergenic
GAAGCGATGCAATGTCAAGA+TGG 0.589336 8.1:+2273411 MS.gene012599:CDS
GGGACATGATGATAGAGAAA+GGG 0.593987 8.1:+2271833 MS.gene012599:CDS
CTTTCACTTGCAGAAAACAT+CGG 0.600525 8.1:-2272955 None:intergenic
TTGGGGTACACTTTGTACCA+TGG 0.600741 8.1:-2273547 None:intergenic
CCTAAAGTGAGAACTGGGAG+AGG 0.607515 8.1:-2273343 None:intergenic
GCTAACAAGAAGAAAATGCA+TGG 0.607753 8.1:+2272029 MS.gene012599:CDS
CTCCAGCAAAAGGGTTCAGT+AGG 0.616386 8.1:+2272748 MS.gene012599:CDS
GATTCTTCGAGATCCAGGTA+AGG 0.621330 8.1:-2272791 None:intergenic
ATGACGAGGAACACTCAACT+CGG 0.622969 8.1:+2272105 MS.gene012599:CDS
ATGTCACTAGATGCATATGT+TGG 0.628893 8.1:-2272829 None:intergenic
GTTAATGGACTATTAGTTTG+GGG 0.635315 8.1:-2273564 None:intergenic
GATAGAGAAAGGGTGATGAA+AGG 0.639508 8.1:+2271843 MS.gene012599:CDS
TCAAGGTATCGTTAGTGATG+GGG 0.646944 8.1:+2271974 MS.gene012599:CDS
GTGATGAAAGGTGTGATGAA+AGG 0.648487 8.1:+2271855 MS.gene012599:CDS
GTAAACATGCCAGAAATGGA+TGG 0.659081 8.1:+2271637 MS.gene012599:CDS
GGATGACACAATAAGTGAAG+CGG 0.659476 8.1:+2273710 MS.gene012599:CDS
GTGACTTGATGAATGTTGAA+GGG 0.669266 8.1:+2272381 MS.gene012599:CDS
TGGAAATTAACCAGTTTCAG+CGG 0.669997 8.1:+2273010 MS.gene012599:CDS
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.1:+2272391 MS.gene012599:CDS
TCATTATCCCAAACAAAACG+CGG 0.696392 8.1:-2272134 None:intergenic
TACTGATGAAGAGAATGACG+AGG 0.706123 8.1:+2272091 MS.gene012599:CDS
GCAAGAGTGATGGAACCACG+TGG 0.841092 8.1:+2273624 MS.gene012599:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAAGGGAAAAAAA+GGG - chr8.1:2271004-2271023 None:intergenic 15.0%
!! AATTAATCATATTTGCAAAA+AGG - chr8.1:2272513-2272532 None:intergenic 15.0%
!! CAAATATGATTAATTTAAAG+GGG + chr8.1:2272518-2272537 MS.gene012599:intron 15.0%
!! CATGTAAACATATAATTAAT+TGG + chr8.1:2270957-2270976 MS.gene012599:intron 15.0%
!! CCGATAAAATAAAATAAAAA+GGG - chr8.1:2271014-2271033 None:intergenic 15.0%
!! GCAAATATGATTAATTTAAA+GGG + chr8.1:2272517-2272536 MS.gene012599:intron 15.0%
!! TAAAATAAAAAGGGAAAAAA+AGG - chr8.1:2271005-2271024 None:intergenic 15.0%
!! TGCAAATATGATTAATTTAA+AGG + chr8.1:2272516-2272535 MS.gene012599:intron 15.0%
!!! CCCTTTTTATTTTATTTTAT+CGG + chr8.1:2271011-2271030 MS.gene012599:intron 15.0%
!!! TGAGATACATTTAAAATAAA+AGG - chr8.1:2271504-2271523 None:intergenic 15.0%
!! AAGATGTAAATTTCAACTTT+AGG - chr8.1:2271351-2271370 None:intergenic 20.0%
!! ATGAAGAAACTACATATATT+AGG - chr8.1:2272328-2272347 None:intergenic 20.0%
!! GCCGATAAAATAAAATAAAA+AGG - chr8.1:2271015-2271034 None:intergenic 20.0%
!! TACTATCTAAATATAAGAAG+TGG - chr8.1:2271769-2271788 None:intergenic 20.0%
!! TGATGATTGATACTTAAATA+GGG + chr8.1:2272291-2272310 MS.gene012599:intron 20.0%
!! TTTAACAATGATCAAATTTC+TGG + chr8.1:2273303-2273322 MS.gene012599:CDS 20.0%
!! TTTAAGTATCAATCATCAAA+TGG - chr8.1:2272289-2272308 None:intergenic 20.0%
!!! AGTGATTATATTAGTTTCTA+GGG + chr8.1:2271060-2271079 MS.gene012599:intron 20.0%
!!! ATTCAACATTTGCTATTATT+GGG + chr8.1:2271448-2271467 MS.gene012599:intron 20.0%
!!! TTAGTGATGATTTTTTCTTT+TGG + chr8.1:2271793-2271812 MS.gene012599:intron 20.0%
!!! TTGATGATTGATACTTAAAT+AGG + chr8.1:2272290-2272309 MS.gene012599:intron 20.0%
! ACACATGTTAAGGAAAATTA+AGG - chr8.1:2271726-2271745 None:intergenic 25.0%
! ATTGAACGAATGAAAAAGAA+AGG - chr8.1:2270879-2270898 None:intergenic 25.0%
! CTATAAGCTATAAGAATGTT+CGG + chr8.1:2271103-2271122 MS.gene012599:intron 25.0%
! CTTAAATTCATGTTACTAAG+TGG - chr8.1:2273045-2273064 None:intergenic 25.0%
! GAATAAAATTTCTCAACTTG+AGG - chr8.1:2273600-2273619 None:intergenic 25.0%
! TAAGTGTGATGTGTATATAT+AGG + chr8.1:2271193-2271212 MS.gene012599:intron 25.0%
! TAATCATATTTGCAAAAAGG+CGG - chr8.1:2272510-2272529 None:intergenic 25.0%
! TATAGGTTATATAAGTCTTC+CGG + chr8.1:2271210-2271229 MS.gene012599:intron 25.0%
! TTGTTAAAATGATCACTCAA+TGG - chr8.1:2273291-2273310 None:intergenic 25.0%
!! AAAGCACTTGTATAATCAAT+TGG - chr8.1:2273069-2273088 None:intergenic 25.0%
!! AAATTCATTCTTTTGCAAGA+TGG + chr8.1:2271304-2271323 MS.gene012599:intron 25.0%
!! GAGTGATTATATTAGTTTCT+AGG + chr8.1:2271059-2271078 MS.gene012599:intron 25.0%
!! TTTGTTAGCTTACTAATGAA+AGG - chr8.1:2271703-2271722 None:intergenic 25.0%
!!! AGTCTGAAACTTTTTACTAA+AGG - chr8.1:2272462-2272481 None:intergenic 25.0%
!!! ATCAAGTCACGTATTTTTTT+AGG - chr8.1:2272371-2272390 None:intergenic 25.0%
!!! ATTACTACTTTTTATTGTGC+CGG + chr8.1:2271377-2271396 MS.gene012599:intron 25.0%
!!! GATTCAACATTTGCTATTAT+TGG + chr8.1:2271447-2271466 MS.gene012599:intron 25.0%
!!! GTCTGAAACTTTTTACTAAA+GGG - chr8.1:2272461-2272480 None:intergenic 25.0%
!!! TCTGAAACTTTTTACTAAAG+GGG - chr8.1:2272460-2272479 None:intergenic 25.0%
AAAAAGAAAGGAACAAAGTG+AGG + chr8.1:2272055-2272074 MS.gene012599:CDS 30.0%
ACACATGTGTTAATTAGCAT+CGG + chr8.1:2271130-2271149 MS.gene012599:intron 30.0%
ACAGTGAGTTTATATGACAA+TGG + chr8.1:2272201-2272220 MS.gene012599:intron 30.0%
AGAGAATGTAAGTGATTGAA+TGG - chr8.1:2271163-2271182 None:intergenic 30.0%
ATACGCTTGCATACAAATAA+TGG + chr8.1:2272558-2272577 MS.gene012599:intron 30.0%
CTTACTTGACAATGTTGTAA+AGG + chr8.1:2273434-2273453 MS.gene012599:CDS 30.0%
GAGAATGTAAGTGATTGAAT+GGG - chr8.1:2271162-2271181 None:intergenic 30.0%
GCTGAATAATACACATGTTA+AGG - chr8.1:2271736-2271755 None:intergenic 30.0%
GTTGAATCAAAATAGTGATG+AGG - chr8.1:2271435-2271454 None:intergenic 30.0%
GTTTCTATAGTTAATCTGCT+TGG + chr8.1:2272173-2272192 MS.gene012599:CDS 30.0%
TACTACAACTACGAATTCAA+TGG + chr8.1:2271558-2271577 MS.gene012599:CDS 30.0%
TGAATCACTACTTTGATTCT+TGG + chr8.1:2271525-2271544 MS.gene012599:intron 30.0%
TTCTTCATCTATTATTGCAG+AGG + chr8.1:2272340-2272359 MS.gene012599:intron 30.0%
! AATTTTCCTAAAGTGAGAAC+TGG - chr8.1:2273352-2273371 None:intergenic 30.0%
! ATTTTCCTAAAGTGAGAACT+GGG - chr8.1:2273351-2273370 None:intergenic 30.0%
! GTAGCAGGATTAAAAGATTT+TGG - chr8.1:2273234-2273253 None:intergenic 30.0%
! GTTAGCAATATTTTCAGATG+AGG - chr8.1:2272015-2272034 None:intergenic 30.0%
! TCTTTTAATCCTGCTACAAA+TGG + chr8.1:2273237-2273256 MS.gene012599:CDS 30.0%
! TTATAATTTGTACCTGCAGA+TGG - chr8.1:2272430-2272449 None:intergenic 30.0%
!! CGTTAATGGACTATTAGTTT+GGG - chr8.1:2273568-2273587 None:intergenic 30.0%
!! GTTAATGGACTATTAGTTTG+GGG - chr8.1:2273567-2273586 None:intergenic 30.0%
!! TCGTTAATGGACTATTAGTT+TGG - chr8.1:2273569-2273588 None:intergenic 30.0%
AAATGATCACTCAATGGAGA+AGG - chr8.1:2273285-2273304 None:intergenic 35.0%
AATAAGTGAAGCGGTTAAAC+AGG + chr8.1:2273719-2273738 MS.gene012599:CDS 35.0%
AATGCATGGACGAAAAAGAA+AGG + chr8.1:2272043-2272062 MS.gene012599:CDS 35.0%
ATAAGAAGCAAGAGAATCCA+AGG - chr8.1:2273511-2273530 None:intergenic 35.0%
ATGTCACTAGATGCATATGT+TGG - chr8.1:2272832-2272851 None:intergenic 35.0%
CACCTATCAACACATAATTC+AGG + chr8.1:2273165-2273184 MS.gene012599:CDS 35.0%
CTGAAACTGGTTAATTTCCA+CGG - chr8.1:2273010-2273029 None:intergenic 35.0%
CTTCTCACATGCAAGTATAA+TGG + chr8.1:2270846-2270865 MS.gene012599:CDS 35.0%
CTTTCACTTGCAGAAAACAT+CGG - chr8.1:2272958-2272977 None:intergenic 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.1:2271957-2271976 MS.gene012599:CDS 35.0%
GAGAAAGAAGATTGAAAGCA+AGG + chr8.1:2271944-2271963 MS.gene012599:CDS 35.0%
GCTAACAAGAAGAAAATGCA+TGG + chr8.1:2272029-2272048 MS.gene012599:CDS 35.0%
GGTTTCTATTACTAATGTGC+CGG - chr8.1:2271399-2271418 None:intergenic 35.0%
GTAAATCACTCTTCTGTTAG+AGG + chr8.1:2273195-2273214 MS.gene012599:CDS 35.0%
GTCATATAAACTCACTGTCA+AGG - chr8.1:2272199-2272218 None:intergenic 35.0%
GTGACTTGATGAATGTTGAA+GGG + chr8.1:2272381-2272400 MS.gene012599:CDS 35.0%
TAACAGTATCTCTGATGTCT+TGG - chr8.1:2273110-2273129 None:intergenic 35.0%
TAATGAAGACACTAGTTCCA+TGG + chr8.1:2273530-2273549 MS.gene012599:CDS 35.0%
TCATTATCCCAAACAAAACG+CGG - chr8.1:2272137-2272156 None:intergenic 35.0%
TCTACTTTAGCCTGTTTAGA+CGG - chr8.1:2272712-2272731 None:intergenic 35.0%
TGATGAGGTTTATAGTGATG+AGG - chr8.1:2271420-2271439 None:intergenic 35.0%
TGATGTAAACATGCCAGAAA+TGG + chr8.1:2271633-2271652 MS.gene012599:CDS 35.0%
TGGAAATTAACCAGTTTCAG+CGG + chr8.1:2273010-2273029 MS.gene012599:CDS 35.0%
TTAGATGAACCATTTGTAGC+AGG - chr8.1:2273249-2273268 None:intergenic 35.0%
TTAGCTTACTAATGAAAGGC+AGG - chr8.1:2271699-2271718 None:intergenic 35.0%
TTATTCCAATGCAAGAGTGA+TGG + chr8.1:2273614-2273633 MS.gene012599:CDS 35.0%
TTCACTTATTGTGTCATCCA+AGG - chr8.1:2273709-2273728 None:intergenic 35.0%
TTCTTCCTTCAACAATACCT+TGG + chr8.1:2273491-2273510 MS.gene012599:CDS 35.0%
TTGGTGTGATATAGTGTTGT+CGG + chr8.1:2271812-2271831 MS.gene012599:intron 35.0%
! AGTGCTTTTACAGTTTCTTC+TGG + chr8.1:2273081-2273100 MS.gene012599:CDS 35.0%
! AGTGTCGAAGAAAAATCAAC+TGG - chr8.1:2273375-2273394 None:intergenic 35.0%
! CATCTAGTGACATGTTTTCT+CGG + chr8.1:2272839-2272858 MS.gene012599:CDS 35.0%
! CTCCTGAATTATGTGTTGAT+AGG - chr8.1:2273170-2273189 None:intergenic 35.0%
!! AATCAAGGTATCGTTAGTGA+TGG + chr8.1:2271972-2271991 MS.gene012599:CDS 35.0%
!! AGAATCCAAGGTATTGTTGA+AGG - chr8.1:2273499-2273518 None:intergenic 35.0%
!! ATCAAGGTATCGTTAGTGAT+GGG + chr8.1:2271973-2271992 MS.gene012599:CDS 35.0%
!!! ATGTTTTGAGATTGAAGTGG+AGG - chr8.1:2272919-2272938 None:intergenic 35.0%
!!! GGAATGTTTTGAGATTGAAG+TGG - chr8.1:2272922-2272941 None:intergenic 35.0%
!!! TGTTTTGAGATTGAAGTGGA+GGG - chr8.1:2272918-2272937 None:intergenic 35.0%
AAAGGCTTCCTGCGTTATAA+AGG - chr8.1:2271486-2271505 None:intergenic 40.0%
AAGAAAGGAACAAAGTGAGG+AGG + chr8.1:2272058-2272077 MS.gene012599:CDS 40.0%
ACCTATATGTAGCTGATGTG+AGG - chr8.1:2271240-2271259 None:intergenic 40.0%
AGTGAAGATGTTACCGAAGA+AGG + chr8.1:2270753-2270772 MS.gene012599:CDS 40.0%
ATCACTCAATGGAGAAGGAT+TGG - chr8.1:2273280-2273299 None:intergenic 40.0%
CCTATATGTAGCTGATGTGA+GGG - chr8.1:2271239-2271258 None:intergenic 40.0%
CGTGACTTGATGAATGTTGA+AGG + chr8.1:2272380-2272399 MS.gene012599:CDS 40.0%
CTTAAGATTCTTCGAGATCC+AGG - chr8.1:2272799-2272818 None:intergenic 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.1:2272391-2272410 MS.gene012599:CDS 40.0%
GATAAATGTTCCGCTGAAAC+TGG - chr8.1:2273023-2273042 None:intergenic 40.0%
GATAGAGAAAGGGTGATGAA+AGG + chr8.1:2271843-2271862 MS.gene012599:CDS 40.0%
GCTATTATTGGGTGAATCGA+CGG + chr8.1:2271459-2271478 MS.gene012599:intron 40.0%
GGATGACACAATAAGTGAAG+CGG + chr8.1:2273710-2273729 MS.gene012599:CDS 40.0%
GGGACATGATGATAGAGAAA+GGG + chr8.1:2271833-2271852 MS.gene012599:CDS 40.0%
GGGTTTGAACAATGACTTCT+TGG + chr8.1:2271080-2271099 MS.gene012599:intron 40.0%
GTAAACATGCCAGAAATGGA+TGG + chr8.1:2271637-2271656 MS.gene012599:CDS 40.0%
TACTGATGAAGAGAATGACG+AGG + chr8.1:2272091-2272110 MS.gene012599:CDS 40.0%
TCAAAAAAGCAATCACCACG+TGG - chr8.1:2273642-2273661 None:intergenic 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.1:2272390-2272409 MS.gene012599:CDS 40.0%
TGAGAATAGTTGTGTGTCCT+TGG + chr8.1:2273689-2273708 MS.gene012599:CDS 40.0%
TGGAGGAGCTTAAGAACATA+TGG + chr8.1:2271910-2271929 MS.gene012599:CDS 40.0%
TGGTGTGATATAGTGTTGTC+GGG + chr8.1:2271813-2271832 MS.gene012599:intron 40.0%
TGTTCTTAAGCTCCTCCAAT+CGG - chr8.1:2271908-2271927 None:intergenic 40.0%
! GAAAGCTTGAGTTTTTCCCA+GGG + chr8.1:2272973-2272992 MS.gene012599:CDS 40.0%
! GTGATGAAAGGTGTGATGAA+AGG + chr8.1:2271855-2271874 MS.gene012599:CDS 40.0%
! TGAAAGCTTGAGTTTTTCCC+AGG + chr8.1:2272972-2272991 MS.gene012599:CDS 40.0%
! TGAGCAAGTTGGACTTGAAA+TGG + chr8.1:2271672-2271691 MS.gene012599:CDS 40.0%
! TGTTACCGAAGAAGGTTTTC+CGG + chr8.1:2270761-2270780 MS.gene012599:CDS 40.0%
! TTTCAAGTCCAACTTGCTCA+AGG - chr8.1:2271672-2271691 None:intergenic 40.0%
!! TCAAGGTATCGTTAGTGATG+GGG + chr8.1:2271974-2271993 MS.gene012599:CDS 40.0%
!! TTCTGTTAGAGGTGCTTATG+TGG + chr8.1:2273206-2273225 MS.gene012599:CDS 40.0%
AAATGTAGCTAGCCATCTGC+AGG + chr8.1:2272415-2272434 MS.gene012599:CDS 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.1:2272757-2272776 MS.gene012599:CDS 45.0%
ACCAACCGGAAAACCTTCTT+CGG - chr8.1:2270769-2270788 None:intergenic 45.0%
AGCTTGAAGCCATCCATTTC+TGG - chr8.1:2271649-2271668 None:intergenic 45.0%
ATGACGAGGAACACTCAACT+CGG + chr8.1:2272105-2272124 MS.gene012599:CDS 45.0%
CCCTCACATCAGCTACATAT+AGG + chr8.1:2271236-2271255 MS.gene012599:intron 45.0%
CGGGACATGATGATAGAGAA+AGG + chr8.1:2271832-2271851 MS.gene012599:CDS 45.0%
GAAGCGATGCAATGTCAAGA+TGG + chr8.1:2273411-2273430 MS.gene012599:CDS 45.0%
GATTCTTCGAGATCCAGGTA+AGG - chr8.1:2272794-2272813 None:intergenic 45.0%
TCTAGTCAAACCGATCCGAT+TGG + chr8.1:2271890-2271909 MS.gene012599:CDS 45.0%
TCTCAAACGACCGTCTAAAC+AGG + chr8.1:2272699-2272718 MS.gene012599:CDS 45.0%
TGGTTCCATCACTCTTGCAT+TGG - chr8.1:2273622-2273641 None:intergenic 45.0%
TTAAGCTCCTCCAATCGGAT+CGG - chr8.1:2271903-2271922 None:intergenic 45.0%
TTCAAGCTCCTTGAGCAAGT+TGG + chr8.1:2271661-2271680 MS.gene012599:CDS 45.0%
TTGGGGTACACTTTGTACCA+TGG - chr8.1:2273550-2273569 None:intergenic 45.0%
! AAACATCGGCTGCTTGAAAC+TGG - chr8.1:2272944-2272963 None:intergenic 45.0%
! AACATCGGCTGCTTGAAACT+GGG - chr8.1:2272943-2272962 None:intergenic 45.0%
! ATCGTTAGTGATGGGGTATG+TGG + chr8.1:2271981-2272000 MS.gene012599:CDS 45.0%
! TGAGGCATCTGAGTCGTTAA+TGG - chr8.1:2273582-2273601 None:intergenic 45.0%
!! GGAAGCGCTTGAATTGTTGA+GGG + chr8.1:2271579-2271598 MS.gene012599:CDS 45.0%
!! TCAGACTAGTCTCAAAGTGC+TGG + chr8.1:2270815-2270834 MS.gene012599:CDS 45.0%
!! TGGAAGCGCTTGAATTGTTG+AGG + chr8.1:2271578-2271597 MS.gene012599:CDS 45.0%
!! TTCGACACTTGCTGTTCCTT+CGG + chr8.1:2273386-2273405 MS.gene012599:CDS 45.0%
!!! GAAGAAGCCGCGTTTTGTTT+GGG + chr8.1:2272127-2272146 MS.gene012599:CDS 45.0%
ACTGGATCCTCTCCAGCAAA+AGG + chr8.1:2272738-2272757 MS.gene012599:CDS 50.0%
AGTCAAACCGATCCGATTGG+AGG + chr8.1:2271893-2271912 MS.gene012599:CDS 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8.1:2272751-2272770 MS.gene012599:CDS 50.0%
CCTAAAGTGAGAACTGGGAG+AGG - chr8.1:2273346-2273365 None:intergenic 50.0%
CCTCTCCCAGTTCTCACTTT+AGG + chr8.1:2273343-2273362 MS.gene012599:CDS 50.0%
CTCCAGCAAAAGGGTTCAGT+AGG + chr8.1:2272748-2272767 MS.gene012599:CDS 50.0%
CTGGATCCTCTCCAGCAAAA+GGG + chr8.1:2272739-2272758 MS.gene012599:CDS 50.0%
GGCTAAAGTAGATGCTGCAC+TGG + chr8.1:2272720-2272739 MS.gene012599:CDS 50.0%
TCGACGGTCCTTTATAACGC+AGG + chr8.1:2271475-2271494 MS.gene012599:intron 50.0%
TGAGATTGAAGTGGAGGGAC+TGG - chr8.1:2272913-2272932 None:intergenic 50.0%
! ACCGAAGAAGGTTTTCCGGT+TGG + chr8.1:2270765-2270784 MS.gene012599:CDS 50.0%
! ACTGAACCCTTTTGCTGGAG+AGG - chr8.1:2272748-2272767 None:intergenic 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8.1:2272753-2272772 None:intergenic 50.0%
! GAGCAACTGCTTGCTCTAGA+TGG + chr8.1:2273660-2273679 MS.gene012599:CDS 50.0%
! GGAGATTTTCGCACCTTACC+TGG + chr8.1:2272778-2272797 MS.gene012599:CDS 50.0%
!! GGAAGAAGCCGCGTTTTGTT+TGG + chr8.1:2272126-2272145 MS.gene012599:CDS 50.0%
CATTGCATCGCTTCCTCCGA+AGG - chr8.1:2273405-2273424 None:intergenic 55.0%
GCCAGAACACGCATACCAAC+CGG - chr8.1:2270783-2270802 None:intergenic 55.0%
GTAGCTGATGTGAGGGACAC+CGG - chr8.1:2271232-2271251 None:intergenic 55.0%
! GCAAGAGTGATGGAACCACG+TGG + chr8.1:2273624-2273643 MS.gene012599:CDS 55.0%
! TCCGGTTGGTATGCGTGTTC+TGG + chr8.1:2270779-2270798 MS.gene012599:CDS 55.0%
!! GACACTTGCTGTTCCTTCGG+AGG + chr8.1:2273389-2273408 MS.gene012599:CDS 55.0%
CACGGATGTTCGAACGCCCT+GGG - chr8.1:2272992-2273011 None:intergenic 60.0%
CCACGGATGTTCGAACGCCC+TGG - chr8.1:2272993-2273012 None:intergenic 65.0%
! CCAGGGCGTTCGAACATCCG+TGG + chr8.1:2272990-2273009 MS.gene012599:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 2270729 2273773 2270729 ID=MS.gene012599
chr8.1 mRNA 2270729 2273773 2270729 ID=MS.gene012599.t1;Parent=MS.gene012599
chr8.1 exon 2270729 2270867 2270729 ID=MS.gene012599.t1.exon1;Parent=MS.gene012599.t1
chr8.1 CDS 2270729 2270867 2270729 ID=cds.MS.gene012599.t1;Parent=MS.gene012599.t1
chr8.1 exon 2271557 2271709 2271557 ID=MS.gene012599.t1.exon2;Parent=MS.gene012599.t1
chr8.1 CDS 2271557 2271709 2271557 ID=cds.MS.gene012599.t1;Parent=MS.gene012599.t1
chr8.1 exon 2271826 2272203 2271826 ID=MS.gene012599.t1.exon3;Parent=MS.gene012599.t1
chr8.1 CDS 2271826 2272203 2271826 ID=cds.MS.gene012599.t1;Parent=MS.gene012599.t1
chr8.1 exon 2272360 2272436 2272360 ID=MS.gene012599.t1.exon4;Parent=MS.gene012599.t1
chr8.1 CDS 2272360 2272436 2272360 ID=cds.MS.gene012599.t1;Parent=MS.gene012599.t1
chr8.1 exon 2272685 2273773 2272685 ID=MS.gene012599.t1.exon5;Parent=MS.gene012599.t1
chr8.1 CDS 2272685 2273773 2272685 ID=cds.MS.gene012599.t1;Parent=MS.gene012599.t1
Gene Sequence

>MS.gene012599

ATGGCTGTGGAGGATCGTTTGGTGAGTGAAGATGTTACCGAAGAAGGTTTTCCGGTTGGTATGCGTGTTCTGGCTGTTGATGACGATCAGACTAGTCTCAAAGTGCTGGAGAAGCATCTTCTCACATGCAAGTATAATGGTTCGTTGCCTTTCTTTTTCATTCGTTCAATTTGATTCTGCTTTGATGATGCTTTCATGTTTTCACTTACTATTCTTCTCTCATGCAATCATGTAAACATATAATTAATTGGAAGTATCTTTGATATAGCAAACCCTTTTTTTCCCTTTTTATTTTATTTTATCGGCAAGTGTTAGTTGTTAGTGATTAGTGAGTGATTATATTAGTTTCTAGGGTTTGAACAATGACTTCTTGGCTATAAGCTATAAGAATGTTCGGACTCACACATGTGTTAATTAGCATCGGACACAACCCATTCAATCACTTACATTCTCTAAAATTATTATAAGTGTGATGTGTATATATAGGTTATATAAGTCTTCCGGTGTCCCTCACATCAGCTACATATAGGTTATATACACACATCAGTCTACAGTTTTCATGAATTTAGATACAAAAATTCATTCTTTTGCAAGATGGCTGTGTTTTAATTTCTATTATCCTAAAGTTGAAATTTACATCTTTTAAAGATTACTACTTTTTATTGTGCCGGCACATTAGTAATAGAAACCTCATCACTATAAACCTCATCACTATTTTGATTCAACATTTGCTATTATTGGGTGAATCGACGGTCCTTTATAACGCAGGAAGCCTTTTATTTTAAATGTATCTCACTGAATCACTACTTTGATTCTTGGTTGTTGCAGTTACTACAACTACGAATTCAATGGAAGCGCTTGAATTGTTGAGGGAAAAGAGAAGTATGTTTGATCTTATAATTAGTGATGTAAACATGCCAGAAATGGATGGCTTCAAGCTCCTTGAGCAAGTTGGACTTGAAATGGACCTGCCTTTCATTAGTAAGCTAACAAACCTTAATTTTCCTTAACATGTGTATTATTCAGCTAATTAAGATCCACTTCTTATATTTAGATAGTATCTTTTAGTGATGATTTTTTCTTTTGGTGTGATATAGTGTTGTCGGGACATGATGATAGAGAAAGGGTGATGAAAGGTGTGATGAAAGGCGCTTGCGACTATCTAGTCAAACCGATCCGATTGGAGGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCGTTAGTGATGGGGTATGTGGTCAAGACACCTCATCTGAAAATATTGCTAACAAGAAGAAAATGCATGGACGAAAAAGAAAGGAACAAAGTGAGGAGGAAGAAGCAGATACTGATGAAGAGAATGACGAGGAACACTCAACTCGGAAGAAGCCGCGTTTTGTTTGGGATAATGAGTTGCACAAAAAATTTGTTTCTATAGTTAATCTGCTTGGCCTTGACAGTGAGTTTATATGACAATGGTTTTTTTTTTTTCTTTTTACTTTGTTAACTTGCTTGACTTGAAAACATGAAACTTAATAAGACCATTTGATGATTGATACTTAAATAGGGATATTATTGTTCCTAATATATGTAGTTTCTTCATCTATTATTGCAGAGGCTTATCCTAAAAAAATACGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTAGCTAGCCATCTGCAGGTACAAATTATAAATAACTTCCCCTTTAGTAAAAAGTTTCAGACTAGTTGATCATTAAAGTATTTGATCACCGCCTTTTTGCAAATATGATTAATTTAAAGGGGATAGTTTACTTTTAAACATACGCTTGCATACAAATAATGGTGTGAAATTTTTTTAGATCAGTTTTTCATTGAATTTCAACAAGCTAGTGTATGAAACAAAAGTATGAAAACAGTGTGTAAGCTCATTCTCGTTTTCGATTGCAGAAATACAGACTTAGTCTCAAACGACCGTCTAAACAGGCTAAAGTAGATGCTGCACTGGATCCTCTCCAGCAAAAGGGTTCAGTAGGTGGATATGGAGATTTTCGCACCTTACCTGGATCTCGAAGAATCTTAAGTAGCACACTGCCAACATATGCATCTAGTGACATGTTTTCTCGGCTGAACGCATCATCTAGTTTGAACTTTAGACGAATGAGTTCTTCTGCACCAGTCCCTCCACTTCAATCTCAAAACATTCCCAGTTTCAAGCAGCCGATGTTTTCTGCAAGTGAAAGCTTGAGTTTTTCCCAGGGCGTTCGAACATCCGTGGAAATTAACCAGTTTCAGCGGAACATTTATCCACTTAGTAACATGAATTTAAGTCCAATTGATTATACAAGTGCTTTTACAGTTTCTTCTGGCTTCCAAGACATCAGAGATACTGTTAATGCAAATAGTTCTCTTTCTTGCATCTCAAGCAGTCACCTATCAACACATAATTCAGGAGCATTTGTAAATCACTCTTCTGTTAGAGGTGCTTATGTGGAACCAAAATCTTTTAATCCTGCTACAAATGGTTCATCTAACTTTGCTTCCAATCCTTCTCCATTGAGTGATCATTTTAACAATGATCAAATTTCTGGCAATAGCTTAAATTTTGCCTCTCCCAGTTCTCACTTTAGGAAAATTCCAGTTGATTTTTCTTCGACACTTGCTGTTCCTTCGGAGGAAGCGATGCAATGTCAAGATGGCTTACTTGACAATGTTGTAAAGGCTTCATGTTACACGACGCAGCAGCAAAATGTCAGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTATAATGAAGACACTAGTTCCATGGTACAAAGTGTACCCCAAACTAATAGTCCATTAACGACTCAGATGCCTCAAGTTGAGAAATTTTATTCCAATGCAAGAGTGATGGAACCACGTGGTGATTGCTTTTTTGAGCAACTGCTTGCTCTAGATGGATTGATTGAGAATAGTTGTGTGTCCTTGGATGACACAATAAGTGAAGCGGTTAAACAGGTATATATGATTAAAAATATTCTTATGTATTAA
Protein sequence

>MS.gene012599.t1

MAVEDRLVSEDVTEEGFPVGMRVLAVDDDQTSLKVLEKHLLTCKYNVTTTTNSMEALELLREKRSMFDLIISDVNMPEMDGFKLLEQVGLEMDLPFIMLSGHDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDQNQGIVSDGVCGQDTSSENIANKKKMHGRKRKEQSEEEEADTDEENDEEHSTRKKPRFVWDNELHKKFVSIVNLLGLDKAYPKKIRDLMNVEGLTRENVASHLQKYRLSLKRPSKQAKVDAALDPLQQKGSVGGYGDFRTLPGSRRILSSTLPTYASSDMFSRLNASSSLNFRRMSSSAPVPPLQSQNIPSFKQPMFSASESLSFSQGVRTSVEINQFQRNIYPLSNMNLSPIDYTSAFTVSSGFQDIRDTVNANSSLSCISSSHLSTHNSGAFVNHSSVRGAYVEPKSFNPATNGSSNFASNPSPLSDHFNNDQISGNSLNFASPSSHFRKIPVDFSSTLAVPSEEAMQCQDGLLDNVVKASCYTTQQQNVSSSFNNTLDSLASYNEDTSSMVQSVPQTNSPLTTQMPQVEKFYSNARVMEPRGDCFFEQLLALDGLIENSCVSLDDTISEAVKQVYMIKNILMY