AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012838

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012838.t1 MTR_3g103590 83.828 303 30 5 1 294 12 304 3.23e-155 436
MS.gene012838.t1 MTR_1g017090 49.147 293 123 11 6 290 11 285 4.14e-73 226
MS.gene012838.t1 MTR_5g014890 59.509 163 59 4 124 286 114 269 7.63e-57 184
MS.gene012838.t1 MTR_7g093430 69.048 126 36 2 123 248 150 272 3.86e-54 178
MS.gene012838.t1 MTR_1g054285 50.249 201 90 4 50 250 78 268 1.70e-53 176
MS.gene012838.t1 MTR_4g097600 64.567 127 42 2 122 248 116 239 2.13e-51 170
MS.gene012838.t1 MTR_2g061030 64.341 129 43 2 124 252 191 316 1.76e-49 167
MS.gene012838.t1 MTR_4g100550 54.305 151 65 2 123 272 110 257 1.30e-48 163
MS.gene012838.t1 MTR_5g013010 56.944 144 58 2 123 265 126 266 1.85e-48 163
MS.gene012838.t1 MTR_8g006705 67.521 117 34 2 132 247 145 258 1.64e-47 161
MS.gene012838.t1 MTR_4g084750 43.386 189 97 3 62 247 1 182 4.75e-42 144
MS.gene012838.t1 MTR_2g038625 49.102 167 74 4 104 264 50 211 1.02e-38 135
MS.gene012838.t1 MTR_2g038580 49.102 167 74 4 104 264 50 211 1.02e-38 135
MS.gene012838.t1 MTR_4g126900 59.130 115 44 3 135 247 90 203 6.20e-32 118
MS.gene012838.t1 MTR_5g013010 69.355 62 19 0 123 184 126 187 2.50e-21 90.1
MS.gene012838.t1 MTR_1g061660 43.820 89 50 0 133 221 59 147 8.23e-19 83.6
MS.gene012838.t1 MTR_6g007647 48.352 91 47 0 130 220 30 120 3.95e-18 82.4
MS.gene012838.t1 MTR_6g007647 48.352 91 47 0 130 220 64 154 4.91e-18 82.4
MS.gene012838.t1 MTR_7g103340 39.604 101 60 1 121 220 50 150 7.66e-17 78.2
MS.gene012838.t1 MTR_5g039000 40.351 114 58 2 121 224 62 175 1.02e-16 79.0
MS.gene012838.t1 MTR_7g010020 40.952 105 55 1 123 220 70 174 1.53e-16 78.2
MS.gene012838.t1 MTR_4g107650 37.879 132 77 1 89 220 56 182 6.27e-16 77.4
MS.gene012838.t1 MTR_5g038280 45.745 94 44 1 133 219 87 180 6.37e-16 77.0
MS.gene012838.t1 MTR_3g086790 40.984 122 64 2 131 245 58 178 1.38e-15 75.9
MS.gene012838.t1 MTR_8g469430 46.809 94 43 1 133 219 89 182 1.41e-15 75.5
MS.gene012838.t1 MTR_8g468210 40.000 100 57 1 124 220 65 164 1.96e-15 75.1
MS.gene012838.t1 MTR_3g080100 44.211 95 46 1 133 220 20 114 1.44e-14 71.6
MS.gene012838.t1 MTR_6g011610 44.681 94 45 1 133 219 96 189 1.59e-14 72.4
MS.gene012838.t1 MTR_3g080100 44.211 95 46 1 133 220 59 153 1.98e-14 72.0
MS.gene012838.t1 MTR_6g011610 44.681 94 45 1 133 219 77 170 3.38e-14 71.2
MS.gene012838.t1 MTR_8g089895 40.909 110 61 1 121 226 42 151 4.60e-14 71.6
MS.gene012838.t1 MTR_8g026960 37.000 100 60 1 137 236 38 134 3.31e-12 65.5
MS.gene012838.t1 MTR_5g019650 43.158 95 49 1 123 217 48 137 1.44e-11 63.2
MS.gene012838.t1 MTR_3g092150 42.353 85 49 0 136 220 41 125 2.10e-11 63.2
MS.gene012838.t1 MTR_5g019680 39.796 98 59 0 129 226 49 146 7.19e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012838.t1 AT5G06710 57.576 165 62 4 89 253 151 307 5.16e-54 179
MS.gene012838.t1 AT4G37790 67.717 127 38 2 122 248 115 238 6.93e-53 174
MS.gene012838.t1 AT2G22800 67.200 125 38 2 124 248 104 225 6.79e-51 169
MS.gene012838.t1 AT4G16780 40.385 260 112 4 42 266 9 260 1.26e-50 169
MS.gene012838.t1 AT3G60390 62.992 127 43 2 122 247 151 274 2.35e-48 164
MS.gene012838.t1 AT5G47370 61.719 128 45 2 122 248 119 243 7.93e-47 159
MS.gene012838.t1 AT4G17460 61.905 126 44 2 124 248 126 248 1.26e-46 158
MS.gene012838.t1 AT2G44910 63.248 117 41 1 132 248 162 276 3.34e-45 155
MS.gene012838.t1 AT2G01430 55.639 133 48 3 122 248 122 249 2.19e-40 142
MS.gene012838.t1 AT1G70920 46.939 147 66 3 121 266 26 161 4.21e-35 125
MS.gene012838.t1 AT1G70920 50.388 129 53 2 121 248 56 174 4.25e-35 126
MS.gene012838.t1 AT5G06710 61.176 85 28 2 100 184 162 241 2.64e-23 96.7
MS.gene012838.t1 AT2G01430 62.319 69 20 2 122 184 122 190 9.12e-19 83.2
MS.gene012838.t1 AT5G03790 40.000 95 57 0 127 221 71 165 1.91e-16 77.4
MS.gene012838.t1 AT3G01470 54.167 72 33 0 133 204 68 139 3.77e-16 77.0
MS.gene012838.t1 AT1G69780 40.541 111 56 2 124 224 73 183 1.66e-15 75.5
MS.gene012838.t1 AT2G36610 35.714 126 66 3 109 221 44 167 3.34e-15 72.8
MS.gene012838.t1 AT2G18550 46.154 91 49 0 129 219 57 147 5.05e-15 73.2
MS.gene012838.t1 AT5G15150 40.741 108 52 2 124 219 101 208 8.05e-15 73.9
MS.gene012838.t1 AT1G26960 37.288 118 69 1 133 250 71 183 1.33e-14 72.4
MS.gene012838.t1 AT5G03790 36.066 122 73 1 127 243 71 192 1.77e-14 70.9
MS.gene012838.t1 AT1G27045 51.389 72 35 0 133 204 68 139 2.47e-14 71.2
MS.gene012838.t1 AT1G27045 51.389 72 35 0 133 204 17 88 2.70e-14 70.1
MS.gene012838.t1 AT1G27045 51.389 72 35 0 133 204 25 96 2.74e-14 70.1
MS.gene012838.t1 AT1G27045 51.389 72 35 0 133 204 25 96 2.74e-14 70.1
MS.gene012838.t1 AT1G27045 51.389 72 35 0 133 204 68 139 3.65e-14 71.2
MS.gene012838.t1 AT4G40060 40.187 107 56 2 131 230 57 162 3.92e-14 71.6
MS.gene012838.t1 AT5G65310 42.857 98 49 1 130 220 51 148 7.26e-14 70.9
MS.gene012838.t1 AT5G65310 42.857 98 49 1 130 220 69 166 8.00e-14 70.9
MS.gene012838.t1 AT3G01220 35.593 118 66 2 99 211 53 165 9.52e-14 70.5
MS.gene012838.t1 AT2G22430 42.268 97 49 1 131 220 60 156 1.02e-13 70.9
MS.gene012838.t1 AT4G36740 40.816 98 58 0 129 226 52 149 1.08e-13 69.3
MS.gene012838.t1 AT4G36740 40.816 98 58 0 129 226 51 148 1.09e-13 69.3
MS.gene012838.t1 AT2G46680 37.895 95 55 1 136 226 35 129 1.09e-11 64.3
MS.gene012838.t1 AT2G46680 40.000 85 51 0 136 220 35 119 2.08e-11 63.5
MS.gene012838.t1 AT3G61890 36.000 100 61 1 136 235 33 129 7.25e-11 61.6

Find 71 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCTTCAATATTATTATT+AGG 0.146207 3.3:-85656911 None:intergenic
TCTCTTCAATATTATTATTA+GGG 0.203525 3.3:-85656910 None:intergenic
TATTTGCATAGAAGTGAATT+TGG 0.206531 3.3:-85658486 None:intergenic
ACTCCTAAGGTAAGAGAATA+TGG 0.209934 3.3:-85656881 None:intergenic
CTGGCTCATGATGTACTTCT+TGG 0.248157 3.3:-85657087 None:intergenic
CAAGATTGAGGATTGTTCTT+TGG 0.279678 3.3:-85657176 None:intergenic
CTCCCTCTTGATGCTATTAG+AGG 0.294156 3.3:-85657047 None:intergenic
TAGAAGTGAATTTGGGCCTT+TGG 0.313028 3.3:-85658478 None:intergenic
TAAACAAAGCAAGAAGATTA+AGG 0.355539 3.3:+85656826 MS.gene012838:CDS
TTTGATATTTCTCTTGGTCT+TGG 0.356272 3.3:+85656779 MS.gene012838:CDS
AGCAATAGTTGCAGAAGATT+TGG 0.363873 3.3:-85658527 None:intergenic
AGAAGTGAATTTGGGCCTTT+GGG 0.375155 3.3:-85658477 None:intergenic
AGAGGAGGATCCATGTAATA+AGG 0.377051 3.3:+85656853 MS.gene012838:CDS
AAATAATTACACGCAGCTTC+CGG 0.391339 3.3:+85658354 MS.gene012838:CDS
AAGCCTAAGTTTCTTCCTAC+TGG 0.393408 3.3:-85657152 None:intergenic
AGCTGCGTGTAATTATTTAC+TGG 0.393707 3.3:-85658349 None:intergenic
AAGAGAAATATCAAAATTCA+TGG 0.395654 3.3:-85656771 None:intergenic
ATTTGCATAGAAGTGAATTT+GGG 0.397943 3.3:-85658485 None:intergenic
CATCACGCTAGTAGAATTAG+AGG 0.402844 3.3:-85657008 None:intergenic
CTCATCGCCGGTGCCGGTGC+CGG 0.407320 3.3:-85658419 None:intergenic
TGGAGGTGTGGTTTCAAAAC+AGG 0.418208 3.3:+85657715 MS.gene012838:CDS
CAAAGAACAATCCTCAATCT+TGG 0.418520 3.3:+85657177 MS.gene012838:CDS
TCAACAAATGAAATCTTCTC+TGG 0.427530 3.3:-85657106 None:intergenic
GAGAGTACAATGATCTTTGA+AGG 0.433397 3.3:-85657206 None:intergenic
GTTTATGTCTTTGTTCATGA+AGG 0.439832 3.3:-85656808 None:intergenic
TCAGAAGCAAAAGCAAGAAT+TGG 0.442692 3.3:+85657662 MS.gene012838:intron
CAAGAAGATTAAGGAGGAAG+AGG 0.445576 3.3:+85656835 MS.gene012838:CDS
ATTTCTCTTGGTCTTGGTCG+TGG 0.454096 3.3:+85656785 MS.gene012838:CDS
CTTGATGCTATTAGAGGAGT+TGG 0.458659 3.3:-85657041 None:intergenic
GCCATTCTCATCGCCGGTGC+CGG 0.463859 3.3:-85658425 None:intergenic
TATTCTCTTACCTTAGGAGT+TGG 0.474125 3.3:+85656884 MS.gene012838:CDS
TGGCAAGCAAGCTGAATCTT+CGG 0.477653 3.3:+85657682 MS.gene012838:CDS
TACTACAACAAAGACTGAAT+CGG 0.478308 3.3:+85656955 MS.gene012838:CDS
TACCCATATTCTCTTACCTT+AGG 0.484324 3.3:+85656878 MS.gene012838:CDS
ATTTGCACCGGCACCGGCAC+CGG 0.484691 3.3:+85658412 MS.gene012838:CDS
GAAGGTGTCCTCCAAGATTG+AGG 0.485914 3.3:-85657188 None:intergenic
AACAGAAGAGAACAAGAGGC+TGG 0.492600 3.3:+85658282 MS.gene012838:CDS
ACCGGCACCGGCGATGAGAA+TGG 0.503337 3.3:+85658424 MS.gene012838:CDS
TATGGGTATGCCTTATTACA+TGG 0.508332 3.3:-85656863 None:intergenic
CTCCTAAGGTAAGAGAATAT+GGG 0.512060 3.3:-85656880 None:intergenic
AAAAGAATTTGCACCGGCAC+CGG 0.514546 3.3:+85658406 MS.gene012838:CDS
AGAGAACAAGAGGCTGGAAG+AGG 0.519798 3.3:+85658288 MS.gene012838:CDS
AATCAAAGCCGTGGTGTGAG+AGG 0.529506 3.3:-85658449 None:intergenic
AAAGCTGAAACAAACGGAAG+TGG 0.530773 3.3:+85658225 MS.gene012838:CDS
GGCTTTGATTCTCTGCCCAA+AGG 0.536458 3.3:+85658462 MS.gene012838:CDS
CTCTTCAATATTATTATTAG+GGG 0.543753 3.3:-85656909 None:intergenic
CTCTAACAGAAGAGAACAAG+AGG 0.544383 3.3:+85658278 MS.gene012838:CDS
CAGGACAAAGCTGAAACAAA+CGG 0.546061 3.3:+85658219 MS.gene012838:intron
AGATCATTGTACTCTCAATC+CGG 0.550528 3.3:+85657213 MS.gene012838:CDS
AGAAGGGCAAACTGTAAGAC+TGG 0.550866 3.3:-85658380 None:intergenic
ACTCCAGTAGGAAGAAACTT+AGG 0.556676 3.3:+85657149 MS.gene012838:CDS
GGACGAGACAAGTGGAGGTG+TGG 0.566994 3.3:+85657703 MS.gene012838:CDS
CAAACTGTAAGACTGGCAAC+CGG 0.573412 3.3:-85658373 None:intergenic
ATTAGGGGATCCAACTCCTA+AGG 0.574104 3.3:-85656894 None:intergenic
GAAGATTAAGGAGGAAGAGG+AGG 0.576607 3.3:+85656838 MS.gene012838:CDS
AGAGGAGCCATTCTCATCGC+CGG 0.585404 3.3:-85658431 None:intergenic
GGTTTCAAAACAGGAGGGCA+AGG 0.585888 3.3:+85657724 MS.gene012838:CDS
AGAACAATCCTCAATCTTGG+AGG 0.594021 3.3:+85657180 MS.gene012838:CDS
AGGTGTGGTTTCAAAACAGG+AGG 0.617414 3.3:+85657718 MS.gene012838:CDS
ACTCCTCTAATAGCATCAAG+AGG 0.625878 3.3:+85657044 MS.gene012838:CDS
ATGTAAATGATAACTCCAGT+AGG 0.626226 3.3:+85657137 MS.gene012838:CDS
TCTTGTAAAAGAATTTGCAC+CGG 0.632422 3.3:+85658400 MS.gene012838:CDS
GAATCTTCGGACGAGACAAG+TGG 0.639016 3.3:+85657695 MS.gene012838:CDS
CTCCTCTAATAGCATCAAGA+GGG 0.641427 3.3:+85657045 MS.gene012838:CDS
ACAAAGCAAGAAGATTAAGG+AGG 0.671987 3.3:+85656829 MS.gene012838:CDS
TGGGCAGAGAATCAAAGCCG+TGG 0.678880 3.3:-85658458 None:intergenic
TCTTCGGACGAGACAAGTGG+AGG 0.693782 3.3:+85657698 MS.gene012838:CDS
ACAAACGGAAGTGGACTGTG+AGG 0.696543 3.3:+85658234 MS.gene012838:CDS
GGTGTGGTTTCAAAACAGGA+GGG 0.700214 3.3:+85657719 MS.gene012838:CDS
GCTTAAGTCCATGAAAACAA+CGG 0.721053 3.3:+85658321 MS.gene012838:CDS
GAATGGCTCCTCTCACACCA+CGG 0.757581 3.3:+85658441 MS.gene012838:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTAATAAAATAAA+TGG - chr3.3:85657447-85657466 None:intergenic 0.0%
!! AAAAAATTAATAAAATAAAT+GGG - chr3.3:85657446-85657465 None:intergenic 0.0%
!!! TATTATTATAAATATTTTTA+TGG + chr3.3:85657797-85657816 MS.gene012838:intron 0.0%
!! TAATATTATGTAAAAAAACA+TGG - chr3.3:85657782-85657801 None:intergenic 10.0%
!!! AAATTGAATAAATTTTTTGA+TGG + chr3.3:85657523-85657542 MS.gene012838:intron 10.0%
!!! TTATAAATATTTTTATGGTT+TGG + chr3.3:85657802-85657821 MS.gene012838:intron 10.0%
!! ATAACATCATATTATATAGT+AGG - chr3.3:85657594-85657613 None:intergenic 15.0%
!! ATTTATTTGAGCAAATTAAT+TGG + chr3.3:85657286-85657305 MS.gene012838:intron 15.0%
!! TAACATCATATTATATAGTA+GGG - chr3.3:85657593-85657612 None:intergenic 15.0%
!! TCTCTTCAATATTATTATTA+GGG - chr3.3:85656913-85656932 None:intergenic 15.0%
!!! CAAAGATATTAGTTTTTTTT+AGG + chr3.3:85657346-85657365 MS.gene012838:intron 15.0%
!! AAGAATTTGAAGATATATAC+CGG - chr3.3:85657235-85657254 None:intergenic 20.0%
!! AAGAGAAATATCAAAATTCA+TGG - chr3.3:85656774-85656793 None:intergenic 20.0%
!! CTCTTCAATATTATTATTAG+GGG - chr3.3:85656912-85656931 None:intergenic 20.0%
!! GAGATATCATAATCATTAAT+CGG - chr3.3:85657988-85658007 None:intergenic 20.0%
!! GTCTCTTCAATATTATTATT+AGG - chr3.3:85656914-85656933 None:intergenic 20.0%
!! GTTATTTATAAGTCATGAAT+TGG + chr3.3:85657609-85657628 MS.gene012838:intron 20.0%
!! TAAATTAACTAACATGTACT+TGG + chr3.3:85657258-85657277 MS.gene012838:intron 20.0%
!! TTAATAAAAATGAGTGCATT+TGG + chr3.3:85657466-85657485 MS.gene012838:intron 20.0%
!! TTACCATATAATATATATGC+AGG + chr3.3:85658200-85658219 MS.gene012838:intron 20.0%
!! TTTAATTACTTTGCTTTACA+AGG - chr3.3:85657506-85657525 None:intergenic 20.0%
!!! AATTATCCTTAAGAGTTTTA+AGG - chr3.3:85657853-85657872 None:intergenic 20.0%
!!! ATGAATTTTGATATTTCTCT+TGG + chr3.3:85656773-85656792 MS.gene012838:CDS 20.0%
!!! ATTAATCTTTTGAATCTTGT+GGG - chr3.3:85657892-85657911 None:intergenic 20.0%
! ATTTGCATAGAAGTGAATTT+GGG - chr3.3:85658488-85658507 None:intergenic 25.0%
! TAAACAAAGCAAGAAGATTA+AGG + chr3.3:85656826-85656845 MS.gene012838:CDS 25.0%
! TATTTGCATAGAAGTGAATT+TGG - chr3.3:85658489-85658508 None:intergenic 25.0%
! TGTCCTGCATATATATTATA+TGG - chr3.3:85658206-85658225 None:intergenic 25.0%
!! GATTAATCTTTTGAATCTTG+TGG - chr3.3:85657893-85657912 None:intergenic 25.0%
!! TGCAAATTCTTTTACAAGAA+GGG - chr3.3:85658399-85658418 None:intergenic 25.0%
!!! TATTTTTATGGTTTGGAATC+CGG + chr3.3:85657809-85657828 MS.gene012838:intron 25.0%
ATGTAAATGATAACTCCAGT+AGG + chr3.3:85657137-85657156 MS.gene012838:CDS 30.0%
CTTTACAAGGACTAAATACA+TGG - chr3.3:85657493-85657512 None:intergenic 30.0%
GTTTATGTCTTTGTTCATGA+AGG - chr3.3:85656811-85656830 None:intergenic 30.0%
TACTACAACAAAGACTGAAT+CGG + chr3.3:85656955-85656974 MS.gene012838:CDS 30.0%
TCAACAAATGAAATCTTCTC+TGG - chr3.3:85657109-85657128 None:intergenic 30.0%
TCTTGTAAAAGAATTTGCAC+CGG + chr3.3:85658400-85658419 MS.gene012838:CDS 30.0%
TTTGATATTTCTCTTGGTCT+TGG + chr3.3:85656779-85656798 MS.gene012838:CDS 30.0%
! GTGCAAATTCTTTTACAAGA+AGG - chr3.3:85658400-85658419 None:intergenic 30.0%
AAATAATTACACGCAGCTTC+CGG + chr3.3:85658354-85658373 MS.gene012838:CDS 35.0%
AAGCGACCTTAAAACTCTTA+AGG + chr3.3:85657844-85657863 MS.gene012838:intron 35.0%
ACAAAGCAAGAAGATTAAGG+AGG + chr3.3:85656829-85656848 MS.gene012838:CDS 35.0%
ACTCCTAAGGTAAGAGAATA+TGG - chr3.3:85656884-85656903 None:intergenic 35.0%
AGATCATTGTACTCTCAATC+CGG + chr3.3:85657213-85657232 MS.gene012838:CDS 35.0%
AGCAATAGTTGCAGAAGATT+TGG - chr3.3:85658530-85658549 None:intergenic 35.0%
AGCTGCGTGTAATTATTTAC+TGG - chr3.3:85658352-85658371 None:intergenic 35.0%
CAAAGAACAATCCTCAATCT+TGG + chr3.3:85657177-85657196 MS.gene012838:CDS 35.0%
CAAGATTGAGGATTGTTCTT+TGG - chr3.3:85657179-85657198 None:intergenic 35.0%
CTCCTAAGGTAAGAGAATAT+GGG - chr3.3:85656883-85656902 None:intergenic 35.0%
GCTTAAGTCCATGAAAACAA+CGG + chr3.3:85658321-85658340 MS.gene012838:CDS 35.0%
TACCCATATTCTCTTACCTT+AGG + chr3.3:85656878-85656897 MS.gene012838:CDS 35.0%
TATTCTCTTACCTTAGGAGT+TGG + chr3.3:85656884-85656903 MS.gene012838:CDS 35.0%
TCAGAAGCAAAAGCAAGAAT+TGG + chr3.3:85657662-85657681 MS.gene012838:intron 35.0%
! GAGAGTACAATGATCTTTGA+AGG - chr3.3:85657209-85657228 None:intergenic 35.0%
! TATGGGTATGCCTTATTACA+TGG - chr3.3:85656866-85656885 None:intergenic 35.0%
AAAGCTGAAACAAACGGAAG+TGG + chr3.3:85658225-85658244 MS.gene012838:CDS 40.0%
AAGCCTAAGTTTCTTCCTAC+TGG - chr3.3:85657155-85657174 None:intergenic 40.0%
ACTCCAGTAGGAAGAAACTT+AGG + chr3.3:85657149-85657168 MS.gene012838:CDS 40.0%
ACTCCTCTAATAGCATCAAG+AGG + chr3.3:85657044-85657063 MS.gene012838:CDS 40.0%
AGAACAATCCTCAATCTTGG+AGG + chr3.3:85657180-85657199 MS.gene012838:CDS 40.0%
AGAAGTGAATTTGGGCCTTT+GGG - chr3.3:85658480-85658499 None:intergenic 40.0%
AGAGGAGGATCCATGTAATA+AGG + chr3.3:85656853-85656872 MS.gene012838:CDS 40.0%
CAAGAAGATTAAGGAGGAAG+AGG + chr3.3:85656835-85656854 MS.gene012838:CDS 40.0%
CAGGACAAAGCTGAAACAAA+CGG + chr3.3:85658219-85658238 MS.gene012838:intron 40.0%
CATCACGCTAGTAGAATTAG+AGG - chr3.3:85657011-85657030 None:intergenic 40.0%
CTCCTCTAATAGCATCAAGA+GGG + chr3.3:85657045-85657064 MS.gene012838:CDS 40.0%
CTCTAACAGAAGAGAACAAG+AGG + chr3.3:85658278-85658297 MS.gene012838:CDS 40.0%
CTTGATGCTATTAGAGGAGT+TGG - chr3.3:85657044-85657063 None:intergenic 40.0%
TAGAAGTGAATTTGGGCCTT+TGG - chr3.3:85658481-85658500 None:intergenic 40.0%
TGCTTCTGATCATGTGTCAA+AGG - chr3.3:85657651-85657670 None:intergenic 40.0%
AACAGAAGAGAACAAGAGGC+TGG + chr3.3:85658282-85658301 MS.gene012838:CDS 45.0%
AGAAGGGCAAACTGTAAGAC+TGG - chr3.3:85658383-85658402 None:intergenic 45.0%
AGGTGTGGTTTCAAAACAGG+AGG + chr3.3:85657718-85657737 MS.gene012838:CDS 45.0%
ATTAGGGGATCCAACTCCTA+AGG - chr3.3:85656897-85656916 None:intergenic 45.0%
CAAACTGTAAGACTGGCAAC+CGG - chr3.3:85658376-85658395 None:intergenic 45.0%
CTCCCTCTTGATGCTATTAG+AGG - chr3.3:85657050-85657069 None:intergenic 45.0%
CTGGCTCATGATGTACTTCT+TGG - chr3.3:85657090-85657109 None:intergenic 45.0%
GAAGATTAAGGAGGAAGAGG+AGG + chr3.3:85656838-85656857 MS.gene012838:CDS 45.0%
GGTGTGGTTTCAAAACAGGA+GGG + chr3.3:85657719-85657738 MS.gene012838:CDS 45.0%
TGGCAAGCAAGCTGAATCTT+CGG + chr3.3:85657682-85657701 MS.gene012838:CDS 45.0%
! AAAAGAATTTGCACCGGCAC+CGG + chr3.3:85658406-85658425 MS.gene012838:CDS 45.0%
! TGGAGGTGTGGTTTCAAAAC+AGG + chr3.3:85657715-85657734 MS.gene012838:CDS 45.0%
!! ATTTCTCTTGGTCTTGGTCG+TGG + chr3.3:85656785-85656804 MS.gene012838:CDS 45.0%
!! TGGTTGTGCCGTTGTTTTCA+TGG - chr3.3:85658332-85658351 None:intergenic 45.0%
!!! AATTGAATAAATTTTTTAAA+TGG - chr3.3:85657420-85657439 None:intergenic 5.0%
AATCAAAGCCGTGGTGTGAG+AGG - chr3.3:85658452-85658471 None:intergenic 50.0%
AGAGAACAAGAGGCTGGAAG+AGG + chr3.3:85658288-85658307 MS.gene012838:CDS 50.0%
GAATCTTCGGACGAGACAAG+TGG + chr3.3:85657695-85657714 MS.gene012838:CDS 50.0%
GGTTTCAAAACAGGAGGGCA+AGG + chr3.3:85657724-85657743 MS.gene012838:CDS 50.0%
GTCGCTTGCATGTCTAACAC+CGG - chr3.3:85657831-85657850 None:intergenic 50.0%
TTGAGCGTGCGAAGGATACT+TGG + chr3.3:85657920-85657939 MS.gene012838:intron 50.0%
! GGCTTTGATTCTCTGCCCAA+AGG + chr3.3:85658462-85658481 MS.gene012838:CDS 50.0%
!! ACAAACGGAAGTGGACTGTG+AGG + chr3.3:85658234-85658253 MS.gene012838:CDS 50.0%
!! GAAGGTGTCCTCCAAGATTG+AGG - chr3.3:85657191-85657210 None:intergenic 50.0%
AGAGGAGCCATTCTCATCGC+CGG - chr3.3:85658434-85658453 None:intergenic 55.0%
ATCCTTCGCACGCTCAACTG+CGG - chr3.3:85657917-85657936 None:intergenic 55.0%
GAATGGCTCCTCTCACACCA+CGG + chr3.3:85658441-85658460 MS.gene012838:CDS 55.0%
TCTTCGGACGAGACAAGTGG+AGG + chr3.3:85657698-85657717 MS.gene012838:CDS 55.0%
TGGGCAGAGAATCAAAGCCG+TGG - chr3.3:85658461-85658480 None:intergenic 55.0%
GGACGAGACAAGTGGAGGTG+TGG + chr3.3:85657703-85657722 MS.gene012838:CDS 60.0%
CTCCGCAGTTGAGCGTGCGA+AGG + chr3.3:85657912-85657931 MS.gene012838:intron 65.0%
GCCATTCTCATCGCCGGTGC+CGG - chr3.3:85658428-85658447 None:intergenic 65.0%
!! ACCGGCACCGGCGATGAGAA+TGG + chr3.3:85658424-85658443 MS.gene012838:CDS 65.0%
!! ATTTGCACCGGCACCGGCAC+CGG + chr3.3:85658412-85658431 MS.gene012838:CDS 65.0%
!! CTCATCGCCGGTGCCGGTGC+CGG - chr3.3:85658422-85658441 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 85656764 85658555 85656764 ID=MS.gene012838
chr3.3 mRNA 85656764 85658555 85656764 ID=MS.gene012838.t1;Parent=MS.gene012838
chr3.3 exon 85656764 85657234 85656764 ID=MS.gene012838.t1.exon1;Parent=MS.gene012838.t1
chr3.3 CDS 85656764 85657234 85656764 ID=cds.MS.gene012838.t1;Parent=MS.gene012838.t1
chr3.3 exon 85657666 85657745 85657666 ID=MS.gene012838.t1.exon2;Parent=MS.gene012838.t1
chr3.3 CDS 85657666 85657745 85657666 ID=cds.MS.gene012838.t1;Parent=MS.gene012838.t1
chr3.3 exon 85658222 85658555 85658222 ID=MS.gene012838.t1.exon3;Parent=MS.gene012838.t1
chr3.3 CDS 85658222 85658555 85658222 ID=cds.MS.gene012838.t1;Parent=MS.gene012838.t1
Gene Sequence

>MS.gene012838

ATGAACACCATGAATTTTGATATTTCTCTTGGTCTTGGTCGTGGCCTTCATGAACAAAGACATAAACAAAGCAAGAAGATTAAGGAGGAAGAGGAGGATCCATGTAATAAGGCATACCCATATTCTCTTACCTTAGGAGTTGGATCCCCTAATAATAATATTGAAGAGACAGATAATCATCAAGTAGCTACTACTACAACAAAGACTGAATCGGATGAGTTTATTTATAATTTGCATCAACGTGCCTCTAATTCTACTAGCGTGATGTCTTCATTTTCCAACTCCTCTAATAGCATCAAGAGGGAGAGAAATGATTTCAACAGCCAAGAAGTACATCATGAGCCAGAGAAGATTTCATTTGTTGATGCTGATGATGTAAATGATAACTCCAGTAGGAAGAAACTTAGGCTTACCAAAGAACAATCCTCAATCTTGGAGGACACCTTCAAAGATCATTGTACTCTCAATCCGGTATATATCTTCAAATTCTTCATTAAATTAACTAACATGTACTTGGTTAGAATTTATTTGAGCAAATTAATTGGTTATAATTAGTGAGCATAATTAAATATGTTTTTTTTACAAAGATATTAGTTTTTTTTAGGAATTAAATGTTTTTAATCTCTATAAAACGATTATTATTTTAATTTAGTCCATTTAAAAAATTTATTCAATTTTACCCATTTATTTTATTAATTTTTTTTAATAAAAATGAGTGCATTTGGTCCATGTATTTAGTCCTTGTAAAGCAAAGTAATTAAATTGAATAAATTTTTTGATGGACTAAATTCAAGTAGTAACTAGTAGAGAGAAAAAACATAATTAACCCTACTATATAATATGATGTTATTTATAAGTCATGAATTGGTTTGATTTCTCATTTTCCTTTGACACATGATCAGAAGCAAAAGCAAGAATTGGCAAGCAAGCTGAATCTTCGGACGAGACAAGTGGAGGTGTGGTTTCAAAACAGGAGGGCAAGGTTGTCATTCTATATTTACAATCATGATTCTCTCCATGTTTTTTTACATAATATTATTATAAATATTTTTATGGTTTGGAATCCGGTGTTAGACATGCAAGCGACCTTAAAACTCTTAAGGATAATTTGTCGTCTATTTAAGTCCCACAAGATTCAAAAGATTAATCTCCGCAGTTGAGCGTGCGAAGGATACTTGGTTAATTTCAACTCAAAATTATTTTAGATATAATAAATTGTCACCGATTAATGATTATGATATCTCATATGCTATCAAACAAATGAAATTATATATTTTCACATAGTAGAATGCAAAATTATTTTAGATATAATAAATTGTCACCGATTAATGATTATGATATCTCATATGCTATCAAACAAATGAAATTATATATTTTCACATAGTAGAATGCATGTGTAAAATTTACACACAAGCAAAATTCTTAAATGTAATCATTTACCATATAATATATATGCAGGACAAAGCTGAAACAAACGGAAGTGGACTGTGAGGCACTGAAGCATTGTTATGAAACTCTAACAGAAGAGAACAAGAGGCTGGAAGAGGAGTTGAAAGAGCTTAAGTCCATGAAAACAACGGCACAACCAGTAAATAATTACACGCAGCTTCCGGTTGCCAGTCTTACAGTTTGCCCTTCTTGTAAAAGAATTTGCACCGGCACCGGCACCGGCGATGAGAATGGCTCCTCTCACACCACGGCTTTGATTCTCTGCCCAAAGGCCCAAATTCACTTCTATGCAAATAATAATAACTATACGTTCGCCAAATCTTCTGCAACTATTGCTAGCTAG
Protein sequence

>MS.gene012838.t1

MNTMNFDISLGLGRGLHEQRHKQSKKIKEEEEDPCNKAYPYSLTLGVGSPNNNIEETDNHQVATTTTKTESDEFIYNLHQRASNSTSVMSSFSNSSNSIKRERNDFNSQEVHHEPEKISFVDADDVNDNSSRKKLRLTKEQSSILEDTFKDHCTLNPKQKQELASKLNLRTRQVEVWFQNRRARTKLKQTEVDCEALKHCYETLTEENKRLEEELKELKSMKTTAQPVNNYTQLPVASLTVCPSCKRICTGTGTGDENGSSHTTALILCPKAQIHFYANNNNYTFAKSSATIAS