AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012873

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012873.t1 MTR_3g103110 63.255 811 136 8 1 660 1 800 0.0 952
MS.gene012873.t1 MTR_7g109640 71.474 631 134 3 53 660 189 796 0.0 870
MS.gene012873.t1 MTR_7g109640 64.634 82 24 1 22 103 74 150 1.42e-18 90.5
MS.gene012873.t1 MTR_3g103120 65.954 608 134 3 53 660 307 841 0.0 762
MS.gene012873.t1 MTR_3g103120 41.139 158 39 2 1 104 1 158 1.43e-20 97.1
MS.gene012873.t1 MTR_5g024390 72.955 440 101 5 225 660 3 428 0.0 664
MS.gene012873.t1 MTR_5g024500 72.500 440 103 5 225 660 3 428 0.0 659
MS.gene012873.t1 MTR_5g071340 40.222 450 234 9 212 653 664 1086 1.57e-105 346
MS.gene012873.t1 MTR_3g464550 37.925 530 282 14 137 651 511 1008 1.37e-104 343
MS.gene012873.t1 MTR_3g103160 40.266 601 188 17 59 653 3 438 1.06e-92 294
MS.gene012873.t1 MTR_2g078040 32.331 532 305 11 138 651 672 1166 1.44e-79 276
MS.gene012873.t1 MTR_1g103830 32.715 431 268 4 222 651 603 1012 2.21e-67 241
MS.gene012873.t1 MTR_1g072130 32.045 440 265 8 222 653 809 1222 3.67e-65 234
MS.gene012873.t1 MTR_7g095140 31.579 323 193 5 340 657 8 307 4.06e-49 176
MS.gene012873.t1 MTR_1g068930 26.013 469 250 15 226 651 441 855 7.24e-43 166
MS.gene012873.t1 MTR_1g068930 26.013 469 250 15 226 651 478 892 7.75e-43 166
MS.gene012873.t1 MTR_7g102610 25.263 475 237 14 251 658 527 950 1.06e-37 150
MS.gene012873.t1 MTR_4g099530 25.339 442 263 14 224 651 110 498 7.23e-34 136
MS.gene012873.t1 MTR_4g057290 26.744 430 206 12 259 623 126 511 8.07e-32 130
MS.gene012873.t1 MTR_1g062330 24.837 459 241 12 259 653 386 804 1.79e-30 128
MS.gene012873.t1 MTR_4g056990 24.351 501 264 15 219 660 184 628 5.18e-30 125
MS.gene012873.t1 MTR_4g050090 24.070 511 263 15 219 654 184 644 6.05e-28 120
MS.gene012873.t1 MTR_6g061000 58.140 86 27 2 312 397 25 101 3.12e-23 95.1
MS.gene012873.t1 MTR_2g084425 29.630 189 112 6 471 655 847 1018 3.14e-18 89.7
MS.gene012873.t1 MTR_3g109988 32.086 187 98 7 443 622 191 355 1.38e-14 76.3
MS.gene012873.t1 MTR_3g110005 32.086 187 98 7 443 622 191 355 1.38e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 367 777 1.34e-101 330
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 598 1008 1.30e-99 328
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 598 1008 1.30e-99 328
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 598 1008 2.64e-99 328
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 589 999 3.27e-99 328
MS.gene012873.t1 AT5G58610 40.741 432 231 6 224 651 589 999 5.04e-99 327
MS.gene012873.t1 AT5G58610 38.605 430 217 6 224 651 589 973 8.42e-88 296
MS.gene012873.t1 AT5G58610 38.605 430 217 6 224 651 589 973 1.12e-87 296
MS.gene012873.t1 AT5G58610 40.263 380 202 6 224 599 598 956 3.93e-84 285
MS.gene012873.t1 AT5G58610 40.263 380 202 6 224 599 589 947 6.55e-84 284
MS.gene012873.t1 AT5G36740 32.054 443 260 8 217 651 540 949 1.12e-69 246
MS.gene012873.t1 AT5G36740 32.054 443 260 8 217 651 540 949 1.12e-69 246
MS.gene012873.t1 AT5G58610 38.889 342 184 6 224 561 589 909 9.15e-69 241
MS.gene012873.t1 AT1G05380 32.494 437 260 5 222 651 517 925 3.19e-68 242
MS.gene012873.t1 AT1G05380 32.494 437 260 5 222 651 517 925 3.19e-68 242
MS.gene012873.t1 AT1G05380 32.494 437 260 5 222 651 517 925 3.19e-68 242
MS.gene012873.t1 AT5G36740 31.473 448 266 8 217 651 387 806 2.30e-67 239
MS.gene012873.t1 AT3G14980 31.364 440 266 8 224 657 575 984 1.82e-64 231
MS.gene012873.t1 AT3G14980 31.364 440 266 8 224 657 620 1029 1.84e-64 231
MS.gene012873.t1 AT3G14980 31.364 440 266 8 224 657 620 1029 1.84e-64 231
MS.gene012873.t1 AT3G14980 31.364 440 266 8 224 657 620 1029 1.84e-64 231
MS.gene012873.t1 AT3G14980 31.364 440 266 8 224 657 620 1029 3.85e-64 229
MS.gene012873.t1 AT5G36670 29.847 459 263 10 217 651 540 963 6.94e-61 221
MS.gene012873.t1 AT5G36670 29.847 459 263 10 217 651 540 963 6.94e-61 221
MS.gene012873.t1 AT4G14920 30.155 451 283 6 210 654 562 986 7.01e-61 221
MS.gene012873.t1 AT4G14920 30.155 451 283 6 210 654 562 986 7.01e-61 221
MS.gene012873.t1 AT4G14920 30.155 451 283 6 210 654 562 986 7.24e-61 221
MS.gene012873.t1 AT5G36670 29.310 464 269 10 217 651 387 820 2.07e-58 213
MS.gene012873.t1 AT5G36740 31.429 385 223 8 217 593 540 891 2.17e-58 212
MS.gene012873.t1 AT5G36740 31.429 385 223 8 217 593 540 891 2.17e-58 212
MS.gene012873.t1 AT5G36670 31.429 385 223 8 217 593 540 891 2.17e-58 212
MS.gene012873.t1 AT5G36670 29.310 464 269 10 217 651 540 973 3.27e-58 213
MS.gene012873.t1 AT5G63900 36.570 309 169 8 348 653 258 542 5.77e-58 205
MS.gene012873.t1 AT3G53680 28.859 447 222 14 226 626 409 805 6.80e-44 169
MS.gene012873.t1 AT3G53680 28.859 447 222 14 226 626 411 807 8.18e-44 169
MS.gene012873.t1 AT3G14980 30.145 345 205 8 224 562 620 934 4.69e-42 164
MS.gene012873.t1 AT2G37520 26.025 488 239 15 226 653 392 817 5.95e-42 163
MS.gene012873.t1 AT2G27980 26.389 504 263 14 219 654 597 1060 2.74e-38 152
MS.gene012873.t1 AT2G36720 25.272 459 239 13 259 655 557 973 9.74e-38 151
MS.gene012873.t1 AT2G36720 25.272 459 239 13 259 655 557 973 9.74e-38 151

Find 117 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACATTGTTGTTTGCATTT+TGG 0.154123 3.3:-85198787 None:intergenic
GTTGCAACTATAAGAATTTA+TGG 0.208645 3.3:+85199270 MS.gene012873:CDS
AAGGCCATATAGGTCTATTT+AGG 0.215735 3.3:-85199551 None:intergenic
CCAACTATTGATGCTTTATC+TGG 0.251503 3.3:+85199138 MS.gene012873:CDS
AACACCATCATTATTTGCTT+TGG 0.281757 3.3:-85198623 None:intergenic
TTCTGTCTGAAGAAGCTATT+AGG 0.283860 3.3:+85198964 MS.gene012873:CDS
AGGTTGCAAATAAAATAAAT+TGG 0.285418 3.3:+85198998 MS.gene012873:CDS
TAGGTCTATTTAGGGATTTA+AGG 0.304954 3.3:-85199542 None:intergenic
GTTTGTTCTATTTGTGGTTT+GGG 0.311387 3.3:+85198643 MS.gene012873:CDS
GCTATTAGGGAAACCAATTA+AGG 0.314529 3.3:+85198978 MS.gene012873:CDS
AAAGGTATTCACTTGTTTCT+TGG 0.322354 3.3:+85198263 MS.gene012873:CDS
TGTGTAAAGGCTACAAGATT+TGG 0.323605 3.3:+85198871 MS.gene012873:CDS
GTTGATAAAGAATCAAGTTT+TGG 0.324712 3.3:+85198285 MS.gene012873:CDS
AGGCCATATAGGTCTATTTA+GGG 0.326281 3.3:-85199550 None:intergenic
AATCCCTAAATAGACCTATA+TGG 0.346948 3.3:+85199547 MS.gene012873:CDS
TGTTTGTTCTATTTGTGGTT+TGG 0.372212 3.3:+85198642 MS.gene012873:CDS
GAAAATGGGTAAAGTCATTT+CGG 0.376245 3.3:+85199248 MS.gene012873:CDS
TATGGCCTTGTATTGCTTGA+TGG 0.376913 3.3:+85199565 MS.gene012873:CDS
CTTAGGTCTCGATCAAGTTC+CGG 0.377173 3.3:+85198717 MS.gene012873:CDS
TTAGGTTCTGGGCAGTTCTC+AGG 0.378707 3.3:-85197641 None:intergenic
CGTGTTAATTTCACTATCAC+TGG 0.385095 3.3:+85198397 MS.gene012873:CDS
CCCTACTTCAGAACTTGCTT+CGG 0.386240 3.3:-85198587 None:intergenic
CCAGATAAAGCATCAATAGT+TGG 0.388520 3.3:-85199138 None:intergenic
AGTAACAATGTTGTCAAAAG+AGG 0.388998 3.3:+85198337 MS.gene012873:CDS
TCAGGTTCTATGTTGAAATC+AGG 0.401611 3.3:-85197623 None:intergenic
AGTATTAAAGATGAGTATAA+TGG 0.402129 3.3:+85198180 MS.gene012873:CDS
GATGGTGTTTGTTCTATTTG+TGG 0.402818 3.3:+85198637 MS.gene012873:CDS
CAAATTGACACTTGTCTTCA+CGG 0.405716 3.3:-85199479 None:intergenic
TTCTTCAATGCAACTCTCGC+AGG 0.405765 3.3:-85197696 None:intergenic
CAATTTCTTCGTCTCTGTTA+CGG 0.406401 3.3:+85197771 MS.gene012873:CDS
ACCATCCACTACTATCTTGT+TGG 0.407099 3.3:+85198453 MS.gene012873:CDS
TGCTATTGCTGCTGCTTCCT+CGG 0.411590 3.3:-85197882 None:intergenic
GCATTCCACCTCGTTTGCTT+AGG 0.414402 3.3:+85198700 MS.gene012873:CDS
GAGAAGAATTCAGAGGTTTA+CGG 0.421103 3.3:+85198202 MS.gene012873:CDS
GCTGCTTCCTCGGATTCTCT+TGG 0.429778 3.3:-85197872 None:intergenic
CCCACCGAATTAGTAACTTC+TGG 0.432390 3.3:-85198082 None:intergenic
TCTGTCTGAAGAAGCTATTA+GGG 0.432712 3.3:+85198965 MS.gene012873:CDS
TTTCACTATCACTGGATTTG+AGG 0.433458 3.3:+85198405 MS.gene012873:CDS
ATCAAGTTCCGGATGGTGAT+TGG 0.433575 3.3:+85198728 MS.gene012873:CDS
CATAAACGCTTGAATTTCCA+TGG 0.434704 3.3:+85199204 MS.gene012873:CDS
TATAAGAATTTATGGTCAAA+AGG 0.441015 3.3:+85199278 MS.gene012873:CDS
TCAGAGTTCGCATGATGAAA+AGG 0.443317 3.3:+85198513 MS.gene012873:CDS
CAGATAAAGCATCAATAGTT+GGG 0.446006 3.3:-85199137 None:intergenic
TGTTGCTTCTGCTGTTTCCT+CGG 0.450642 3.3:-85197816 None:intergenic
CAGAGTTCGCATGATGAAAA+GGG 0.457917 3.3:+85198514 MS.gene012873:CDS
GAAATTGCTGCTACTGCTAC+TGG 0.465818 3.3:-85197755 None:intergenic
TGAACCAGAAGTTACTAATT+CGG 0.467506 3.3:+85198078 MS.gene012873:CDS
CAAGCAATACAAGGCCATAT+AGG 0.468400 3.3:-85199561 None:intergenic
CTGCAGTCGAAGGGTGTTTG+AGG 0.471352 3.3:-85197730 None:intergenic
GACACGTGGACAAAAGCGTT+TGG 0.477202 3.3:+85199441 MS.gene012873:CDS
GAGAAACAGTTGACACGCTT+AGG 0.478300 3.3:+85199378 MS.gene012873:CDS
AGGCTCATGCTGGTTGTACA+AGG 0.491245 3.3:+85198425 MS.gene012873:CDS
AAATTGACACTTGTCTTCAC+GGG 0.497364 3.3:-85199478 None:intergenic
TACTACGGTGTCAACATCAA+TGG 0.502626 3.3:+85198030 MS.gene012873:CDS
TTGTCTTCGATATTGTTCCT+TGG 0.503462 3.3:-85199325 None:intergenic
ATCATCAGAAGTTGATTCAT+CGG 0.513368 3.3:-85197918 None:intergenic
GGAAGACGGAAGGTCATTGT+TGG 0.514903 3.3:+85198474 MS.gene012873:CDS
ACTGGATTTGAGGCTCATGC+TGG 0.514944 3.3:+85198415 MS.gene012873:CDS
TATCAAAGACGGAAGGTACA+AGG 0.515719 3.3:+85198239 MS.gene012873:CDS
GCTACTGGTGCTGCAGTCGA+AGG 0.520375 3.3:-85197740 None:intergenic
CCACGTGTCTATAGCATCCT+CGG 0.528623 3.3:-85199427 None:intergenic
CCGAAGCAAGTTCTGAAGTA+GGG 0.528703 3.3:+85198587 MS.gene012873:CDS
TCTTCAATGCAACTCTCGCA+GGG 0.531694 3.3:-85197695 None:intergenic
GTTACTAATTCGGTGGGTAG+TGG 0.540215 3.3:+85198088 MS.gene012873:CDS
TGCAGTCGAAGGGTGTTTGA+GGG 0.545311 3.3:-85197729 None:intergenic
TCCGAAGCAAGTTCTGAAGT+AGG 0.548155 3.3:+85198586 MS.gene012873:CDS
ACGCTTAGGAGTTGAAAGCT+TGG 0.553379 3.3:+85199392 MS.gene012873:CDS
CAGACGATTGTGGTAGAAGA+GGG 0.555231 3.3:+85198155 MS.gene012873:CDS
TGCGAGAGTTGCATTGAAGA+AGG 0.557070 3.3:+85197698 MS.gene012873:CDS
AACTACCATCAAGCAATACA+AGG 0.564139 3.3:-85199570 None:intergenic
GGACAAAACCAATCACCATC+CGG 0.566171 3.3:-85198736 None:intergenic
CAGAGGTTTACGGTAAGAGA+GGG 0.567218 3.3:+85198212 MS.gene012873:CDS
TCGGTGGGTAGTGGTAAAAG+AGG 0.571961 3.3:+85198097 MS.gene012873:CDS
AAATCACATTATAGAAACCA+TGG 0.572412 3.3:-85199221 None:intergenic
GGTAGCCACGAAAACTATCT+CGG 0.575564 3.3:-85199304 None:intergenic
TATAATGGAGAAGAATTCAG+AGG 0.575599 3.3:+85198195 MS.gene012873:CDS
TCAGACGATTGTGGTAGAAG+AGG 0.577488 3.3:+85198154 MS.gene012873:CDS
TCAGAGGTTTACGGTAAGAG+AGG 0.577597 3.3:+85198211 MS.gene012873:CDS
GTAACAATGTTGTCAAAAGA+GGG 0.581494 3.3:+85198338 MS.gene012873:CDS
TGTTCTATTTGTGGTTTGGG+AGG 0.585455 3.3:+85198646 MS.gene012873:CDS
ACTACTATCTTGTTGGAAGA+CGG 0.590421 3.3:+85198460 MS.gene012873:CDS
AAAGCGACCAAGAGAATCCG+AGG 0.592053 3.3:+85197865 MS.gene012873:CDS
AGACGGAAGGTACAAGGGAA+AGG 0.593986 3.3:+85198245 MS.gene012873:CDS
GAGAGGGAAGGTATCAAAGA+CGG 0.594876 3.3:+85198228 MS.gene012873:CDS
CTACTGGTGCTGCAGTCGAA+GGG 0.599740 3.3:-85197739 None:intergenic
GTTTCCAAAGCAAATAATGA+TGG 0.599744 3.3:+85198619 MS.gene012873:CDS
ACCAGAAGTTACTAATTCGG+TGG 0.600989 3.3:+85198081 MS.gene012873:CDS
AAAGAGATTGAGAGAATCCG+AGG 0.612293 3.3:+85197799 MS.gene012873:CDS
GAGAAGAATTCAGACGATTG+TGG 0.613172 3.3:+85198145 MS.gene012873:CDS
GTCATTGTTGGAATGTCAGA+GGG 0.614589 3.3:+85198486 MS.gene012873:CDS
GTCTTCCAACAAGATAGTAG+TGG 0.619064 3.3:-85198458 None:intergenic
GGTCATTGTTGGAATGTCAG+AGG 0.622266 3.3:+85198485 MS.gene012873:CDS
AAAGAAGAGAAAGTTGAATG+TGG 0.626130 3.3:+85199086 MS.gene012873:CDS
CCAGAAGTTACTAATTCGGT+GGG 0.626761 3.3:+85198082 MS.gene012873:CDS
ATCAAAGACGGAAGGTACAA+GGG 0.629537 3.3:+85198240 MS.gene012873:CDS
TTCTGAATCAACTTCTGACG+AGG 0.630454 3.3:+85197964 MS.gene012873:CDS
TCCAACAAGATAGTAGTGGA+TGG 0.630549 3.3:-85198454 None:intergenic
CAAACGAGGTGGAATGCAGA+AGG 0.631169 3.3:-85198694 None:intergenic
GAGAGTTGCATTGAAGAAGG+AGG 0.635933 3.3:+85197701 MS.gene012873:CDS
GGGTAGTGGTAAAAGAGGAA+AGG 0.636646 3.3:+85198102 MS.gene012873:CDS
AGAGTTGCATTGAAGAAGGA+GGG 0.637310 3.3:+85197702 MS.gene012873:CDS
CTATCTTGTTGGAAGACGGA+AGG 0.639850 3.3:+85198464 MS.gene012873:CDS
AGAGTTCGCATGATGAAAAG+GGG 0.652963 3.3:+85198515 MS.gene012873:CDS
TCTATGTTGAAATCAGGAGG+TGG 0.653673 3.3:-85197617 None:intergenic
GGTTCTATGTTGAAATCAGG+AGG 0.654128 3.3:-85197620 None:intergenic
GGTTTACGGTAAGAGAGGGA+AGG 0.658054 3.3:+85198216 MS.gene012873:CDS
AAGATTTGGATCTAGTCACA+TGG 0.660288 3.3:+85198885 MS.gene012873:CDS
AATTTGAGTAGTGATGATGA+TGG 0.661624 3.3:+85199033 MS.gene012873:CDS
AAGGAACAATATCGAAGACA+AGG 0.663403 3.3:+85199327 MS.gene012873:CDS
GGGAAGGTATCAAAGACGGA+AGG 0.677116 3.3:+85198232 MS.gene012873:CDS
CCGAGGATGCTATAGACACG+TGG 0.685477 3.3:+85199427 MS.gene012873:CDS
TTGAGTAGTGATGATGATGG+CGG 0.685672 3.3:+85199036 MS.gene012873:CDS
GGTCTCGATCAAGTTCCGGA+TGG 0.685979 3.3:+85198721 MS.gene012873:CDS
GATCGAGACCTAAGCAAACG+AGG 0.703690 3.3:-85198708 None:intergenic
CGAGACCTAAGCAAACGAGG+TGG 0.726299 3.3:-85198705 None:intergenic
CGTGTCTATAGCATCCTCGG+AGG 0.738184 3.3:-85199424 None:intergenic
TGAAAAGGGGAATCATTGTG+TGG 0.749018 3.3:+85198528 MS.gene012873:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGTTGCAAATAAAATAAAT+TGG + chr3.3:85198998-85199017 MS.gene012873:CDS 20.0%
!! AGTATTAAAGATGAGTATAA+TGG + chr3.3:85198180-85198199 MS.gene012873:CDS 20.0%
!! TATAAGAATTTATGGTCAAA+AGG + chr3.3:85199278-85199297 MS.gene012873:CDS 20.0%
!!! AATGTGATTTTAGAGAAAAT+GGG + chr3.3:85199234-85199253 MS.gene012873:CDS 20.0%
!!! ATTTTATTTGCAACCTTAAT+TGG - chr3.3:85198994-85199013 None:intergenic 20.0%
!!! TAATGTGATTTTAGAGAAAA+TGG + chr3.3:85199233-85199252 MS.gene012873:CDS 20.0%
! AAATCACATTATAGAAACCA+TGG - chr3.3:85199224-85199243 None:intergenic 25.0%
! CAGTTCGATTAAAAAATCTA+CGG + chr3.3:85198006-85198025 MS.gene012873:CDS 25.0%
! GTTGCAACTATAAGAATTTA+TGG + chr3.3:85199270-85199289 MS.gene012873:CDS 25.0%
! TAAAAAATCTACGGTTACTA+CGG + chr3.3:85198015-85198034 MS.gene012873:CDS 25.0%
!! TCTAAGAGAAATGTAGTTTT+TGG - chr3.3:85197672-85197691 None:intergenic 25.0%
!!! GGTTTTAAAAATGAGTACAA+TGG + chr3.3:85198123-85198142 MS.gene012873:CDS 25.0%
!!! GTTGATAAAGAATCAAGTTT+TGG + chr3.3:85198285-85198304 MS.gene012873:CDS 25.0%
AAAGAAGAGAAAGTTGAATG+TGG + chr3.3:85199086-85199105 MS.gene012873:CDS 30.0%
AACACCATCATTATTTGCTT+TGG - chr3.3:85198626-85198645 None:intergenic 30.0%
AATCCCTAAATAGACCTATA+TGG + chr3.3:85199547-85199566 MS.gene012873:CDS 30.0%
AGTAACAATGTTGTCAAAAG+AGG + chr3.3:85198337-85198356 MS.gene012873:CDS 30.0%
GAAAATGGGTAAAGTCATTT+CGG + chr3.3:85199248-85199267 MS.gene012873:CDS 30.0%
GTAACAATGTTGTCAAAAGA+GGG + chr3.3:85198338-85198357 MS.gene012873:CDS 30.0%
GTTTCCAAAGCAAATAATGA+TGG + chr3.3:85198619-85198638 MS.gene012873:CDS 30.0%
GTTTGTTCTATTTGTGGTTT+GGG + chr3.3:85198643-85198662 MS.gene012873:CDS 30.0%
TAGGTCTATTTAGGGATTTA+AGG - chr3.3:85199545-85199564 None:intergenic 30.0%
TGAACCAGAAGTTACTAATT+CGG + chr3.3:85198078-85198097 MS.gene012873:CDS 30.0%
TGTTTGTTCTATTTGTGGTT+TGG + chr3.3:85198642-85198661 MS.gene012873:CDS 30.0%
! AATTTGAGTAGTGATGATGA+TGG + chr3.3:85199033-85199052 MS.gene012873:CDS 30.0%
! ATCATCAGAAGTTGATTCAT+CGG - chr3.3:85197921-85197940 None:intergenic 30.0%
! CAGATAAAGCATCAATAGTT+GGG - chr3.3:85199140-85199159 None:intergenic 30.0%
! CATATTTTGCAACAACAAGT+TGG - chr3.3:85198760-85198779 None:intergenic 30.0%
! TATAATGGAGAAGAATTCAG+AGG + chr3.3:85198195-85198214 MS.gene012873:CDS 30.0%
! TGTGAGCAAAATTTTCACTT+TGG + chr3.3:85198847-85198866 MS.gene012873:CDS 30.0%
! TTTTCACTTTGGATGTGTAA+AGG + chr3.3:85198858-85198877 MS.gene012873:CDS 30.0%
!! AAAGGTATTCACTTGTTTCT+TGG + chr3.3:85198263-85198282 MS.gene012873:CDS 30.0%
!!! AAAGATGTCGTTTTTAGTAG+AGG + chr3.3:85199174-85199193 MS.gene012873:CDS 30.0%
!!! AAGAATCAAGTTTTGGTTTC+TGG + chr3.3:85198292-85198311 MS.gene012873:CDS 30.0%
!!! AGAATCAAGTTTTGGTTTCT+GGG + chr3.3:85198293-85198312 MS.gene012873:CDS 30.0%
!!! AGAGGGAGTTTATTTTATGA+TGG + chr3.3:85198355-85198374 MS.gene012873:CDS 30.0%
!!! ATGTAGTTTTTGGCATTGTT+AGG - chr3.3:85197662-85197681 None:intergenic 30.0%
!!! CACATTGTTGTTTGCATTTT+GGG - chr3.3:85198789-85198808 None:intergenic 30.0%
AAATTGACACTTGTCTTCAC+GGG - chr3.3:85199481-85199500 None:intergenic 35.0%
AAGATTTGGATCTAGTCACA+TGG + chr3.3:85198885-85198904 MS.gene012873:CDS 35.0%
AAGGAACAATATCGAAGACA+AGG + chr3.3:85199327-85199346 MS.gene012873:CDS 35.0%
AAGGCCATATAGGTCTATTT+AGG - chr3.3:85199554-85199573 None:intergenic 35.0%
ACTACTATCTTGTTGGAAGA+CGG + chr3.3:85198460-85198479 MS.gene012873:CDS 35.0%
AGGCCATATAGGTCTATTTA+GGG - chr3.3:85199553-85199572 None:intergenic 35.0%
CAAATTGACACTTGTCTTCA+CGG - chr3.3:85199482-85199501 None:intergenic 35.0%
CAATTTCTTCGTCTCTGTTA+CGG + chr3.3:85197771-85197790 MS.gene012873:CDS 35.0%
CATAAACGCTTGAATTTCCA+TGG + chr3.3:85199204-85199223 MS.gene012873:CDS 35.0%
CCAACTATTGATGCTTTATC+TGG + chr3.3:85199138-85199157 MS.gene012873:CDS 35.0%
CGTGTTAATTTCACTATCAC+TGG + chr3.3:85198397-85198416 MS.gene012873:CDS 35.0%
GAGAAGAATTCAGAGGTTTA+CGG + chr3.3:85198202-85198221 MS.gene012873:CDS 35.0%
GATGGTGTTTGTTCTATTTG+TGG + chr3.3:85198637-85198656 MS.gene012873:CDS 35.0%
GCAATGTTAAAAACAGGAAC+TGG + chr3.3:85198911-85198930 MS.gene012873:CDS 35.0%
GCTATTAGGGAAACCAATTA+AGG + chr3.3:85198978-85198997 MS.gene012873:CDS 35.0%
TCAGGTTCTATGTTGAAATC+AGG - chr3.3:85197626-85197645 None:intergenic 35.0%
TCTGTCTGAAGAAGCTATTA+GGG + chr3.3:85198965-85198984 MS.gene012873:CDS 35.0%
TGGAGAGCAATGTTAAAAAC+AGG + chr3.3:85198905-85198924 MS.gene012873:CDS 35.0%
TGTGTAAAGGCTACAAGATT+TGG + chr3.3:85198871-85198890 MS.gene012873:CDS 35.0%
TTCTGTCTGAAGAAGCTATT+AGG + chr3.3:85198964-85198983 MS.gene012873:CDS 35.0%
TTGTCTTCGATATTGTTCCT+TGG - chr3.3:85199328-85199347 None:intergenic 35.0%
TTTCACTATCACTGGATTTG+AGG + chr3.3:85198405-85198424 MS.gene012873:CDS 35.0%
! CCAGATAAAGCATCAATAGT+TGG - chr3.3:85199141-85199160 None:intergenic 35.0%
! TTTTGGCATTGTTAGGTTCT+GGG - chr3.3:85197655-85197674 None:intergenic 35.0%
!! TTTTTGGCATTGTTAGGTTC+TGG - chr3.3:85197656-85197675 None:intergenic 35.0%
!!! GCACATTGTTGTTTGCATTT+TGG - chr3.3:85198790-85198809 None:intergenic 35.0%
AAAGAGATTGAGAGAATCCG+AGG + chr3.3:85197799-85197818 MS.gene012873:CDS 40.0%
ACCAGAAGTTACTAATTCGG+TGG + chr3.3:85198081-85198100 MS.gene012873:CDS 40.0%
ACCATCCACTACTATCTTGT+TGG + chr3.3:85198453-85198472 MS.gene012873:CDS 40.0%
AGAGTTCGCATGATGAAAAG+GGG + chr3.3:85198515-85198534 MS.gene012873:CDS 40.0%
AGAGTTGCATTGAAGAAGGA+GGG + chr3.3:85197702-85197721 MS.gene012873:CDS 40.0%
CAAGCAATACAAGGCCATAT+AGG - chr3.3:85199564-85199583 None:intergenic 40.0%
CAGAGTTCGCATGATGAAAA+GGG + chr3.3:85198514-85198533 MS.gene012873:CDS 40.0%
CCAGAAGTTACTAATTCGGT+GGG + chr3.3:85198082-85198101 MS.gene012873:CDS 40.0%
GAGAAGAATTCAGACGATTG+TGG + chr3.3:85198145-85198164 MS.gene012873:CDS 40.0%
GGTTCTATGTTGAAATCAGG+AGG - chr3.3:85197623-85197642 None:intergenic 40.0%
GTCATTGTTGGAATGTCAGA+GGG + chr3.3:85198486-85198505 MS.gene012873:CDS 40.0%
GTCTTCCAACAAGATAGTAG+TGG - chr3.3:85198461-85198480 None:intergenic 40.0%
TCAGAGTTCGCATGATGAAA+AGG + chr3.3:85198513-85198532 MS.gene012873:CDS 40.0%
TCTATGTTGAAATCAGGAGG+TGG - chr3.3:85197620-85197639 None:intergenic 40.0%
TGAAAAGGGGAATCATTGTG+TGG + chr3.3:85198528-85198547 MS.gene012873:CDS 40.0%
TTCTGAATCAACTTCTGACG+AGG + chr3.3:85197964-85197983 MS.gene012873:CDS 40.0%
! ATCAAAGACGGAAGGTACAA+GGG + chr3.3:85198240-85198259 MS.gene012873:CDS 40.0%
! TACTACGGTGTCAACATCAA+TGG + chr3.3:85198030-85198049 MS.gene012873:CDS 40.0%
! TATCAAAGACGGAAGGTACA+AGG + chr3.3:85198239-85198258 MS.gene012873:CDS 40.0%
! TCCAACAAGATAGTAGTGGA+TGG - chr3.3:85198457-85198476 None:intergenic 40.0%
! TGTTCTATTTGTGGTTTGGG+AGG + chr3.3:85198646-85198665 MS.gene012873:CDS 40.0%
!! TGGTTTTGCAGTGTAGTTTG+CGG + chr3.3:85198931-85198950 MS.gene012873:CDS 40.0%
!! TTGAGTAGTGATGATGATGG+CGG + chr3.3:85199036-85199055 MS.gene012873:CDS 40.0%
!!! CAAAAGCGTTTGGTTTTGCT+AGG + chr3.3:85199451-85199470 MS.gene012873:CDS 40.0%
ACGCTTAGGAGTTGAAAGCT+TGG + chr3.3:85199392-85199411 MS.gene012873:CDS 45.0%
ATCAAGTTCCGGATGGTGAT+TGG + chr3.3:85198728-85198747 MS.gene012873:CDS 45.0%
CAGACGATTGTGGTAGAAGA+GGG + chr3.3:85198155-85198174 MS.gene012873:CDS 45.0%
CAGAGGTTTACGGTAAGAGA+GGG + chr3.3:85198212-85198231 MS.gene012873:CDS 45.0%
CCCACCGAATTAGTAACTTC+TGG - chr3.3:85198085-85198104 None:intergenic 45.0%
CTATCTTGTTGGAAGACGGA+AGG + chr3.3:85198464-85198483 MS.gene012873:CDS 45.0%
CTTAGGTCTCGATCAAGTTC+CGG + chr3.3:85198717-85198736 MS.gene012873:CDS 45.0%
GAAATTGCTGCTACTGCTAC+TGG - chr3.3:85197758-85197777 None:intergenic 45.0%
GAGAAACAGTTGACACGCTT+AGG + chr3.3:85199378-85199397 MS.gene012873:CDS 45.0%
GAGAGTTGCATTGAAGAAGG+AGG + chr3.3:85197701-85197720 MS.gene012873:CDS 45.0%
GGACAAAACCAATCACCATC+CGG - chr3.3:85198739-85198758 None:intergenic 45.0%
GGTAGCCACGAAAACTATCT+CGG - chr3.3:85199307-85199326 None:intergenic 45.0%
GGTCATTGTTGGAATGTCAG+AGG + chr3.3:85198485-85198504 MS.gene012873:CDS 45.0%
TCAGACGATTGTGGTAGAAG+AGG + chr3.3:85198154-85198173 MS.gene012873:CDS 45.0%
TCAGAGGTTTACGGTAAGAG+AGG + chr3.3:85198211-85198230 MS.gene012873:CDS 45.0%
TCTTCAATGCAACTCTCGCA+GGG - chr3.3:85197698-85197717 None:intergenic 45.0%
TGCGAGAGTTGCATTGAAGA+AGG + chr3.3:85197698-85197717 MS.gene012873:CDS 45.0%
TTCTTCAATGCAACTCTCGC+AGG - chr3.3:85197699-85197718 None:intergenic 45.0%
! CCCTACTTCAGAACTTGCTT+CGG - chr3.3:85198590-85198609 None:intergenic 45.0%
! GATAGTTTTCGTGGCTACCA+AGG + chr3.3:85199308-85199327 MS.gene012873:CDS 45.0%
! GGGTAGTGGTAAAAGAGGAA+AGG + chr3.3:85198102-85198121 MS.gene012873:CDS 45.0%
! GTTACTAATTCGGTGGGTAG+TGG + chr3.3:85198088-85198107 MS.gene012873:CDS 45.0%
!! CCGAAGCAAGTTCTGAAGTA+GGG + chr3.3:85198587-85198606 MS.gene012873:CDS 45.0%
!! GAGAGGGAAGGTATCAAAGA+CGG + chr3.3:85198228-85198247 MS.gene012873:CDS 45.0%
!! GGGACTACTGTGTTTTGTTG+TGG + chr3.3:85198313-85198332 MS.gene012873:CDS 45.0%
!! GGTTTTGCAGTGTAGTTTGC+GGG + chr3.3:85198932-85198951 MS.gene012873:CDS 45.0%
!! TCCGAAGCAAGTTCTGAAGT+AGG + chr3.3:85198586-85198605 MS.gene012873:CDS 45.0%
!! TGTTGCTTCTGCTGTTTCCT+CGG - chr3.3:85197819-85197838 None:intergenic 45.0%
AAAGCGACCAAGAGAATCCG+AGG + chr3.3:85197865-85197884 MS.gene012873:CDS 50.0%
CAAACGAGGTGGAATGCAGA+AGG - chr3.3:85198697-85198716 None:intergenic 50.0%
CCACGTGTCTATAGCATCCT+CGG - chr3.3:85199430-85199449 None:intergenic 50.0%
GATCGAGACCTAAGCAAACG+AGG - chr3.3:85198711-85198730 None:intergenic 50.0%
GCATTCCACCTCGTTTGCTT+AGG + chr3.3:85198700-85198719 MS.gene012873:CDS 50.0%
GGAAGACGGAAGGTCATTGT+TGG + chr3.3:85198474-85198493 MS.gene012873:CDS 50.0%
GGTTTACGGTAAGAGAGGGA+AGG + chr3.3:85198216-85198235 MS.gene012873:CDS 50.0%
! ACTGGATTTGAGGCTCATGC+TGG + chr3.3:85198415-85198434 MS.gene012873:CDS 50.0%
! AGACGGAAGGTACAAGGGAA+AGG + chr3.3:85198245-85198264 MS.gene012873:CDS 50.0%
! AGGCTCATGCTGGTTGTACA+AGG + chr3.3:85198425-85198444 MS.gene012873:CDS 50.0%
! GGTTGCCGAGATAGTTTTCG+TGG + chr3.3:85199299-85199318 MS.gene012873:CDS 50.0%
! TCGGTGGGTAGTGGTAAAAG+AGG + chr3.3:85198097-85198116 MS.gene012873:CDS 50.0%
! TGCTATTGCTGCTGCTTCCT+CGG - chr3.3:85197885-85197904 None:intergenic 50.0%
!! GACACGTGGACAAAAGCGTT+TGG + chr3.3:85199441-85199460 MS.gene012873:CDS 50.0%
!! GGGAAGGTATCAAAGACGGA+AGG + chr3.3:85198232-85198251 MS.gene012873:CDS 50.0%
!! TGCAGTCGAAGGGTGTTTGA+GGG - chr3.3:85197732-85197751 None:intergenic 50.0%
!! TTAGGTTCTGGGCAGTTCTC+AGG - chr3.3:85197644-85197663 None:intergenic 50.0%
!!! CTTGGTTTTGCATTCCTCCG+AGG + chr3.3:85199410-85199429 MS.gene012873:CDS 50.0%
CCGAGGATGCTATAGACACG+TGG + chr3.3:85199427-85199446 MS.gene012873:CDS 55.0%
CGAGACCTAAGCAAACGAGG+TGG - chr3.3:85198708-85198727 None:intergenic 55.0%
CGTGTCTATAGCATCCTCGG+AGG - chr3.3:85199427-85199446 None:intergenic 55.0%
GCTGCTTCCTCGGATTCTCT+TGG - chr3.3:85197875-85197894 None:intergenic 55.0%
GGTCTCGATCAAGTTCCGGA+TGG + chr3.3:85198721-85198740 MS.gene012873:CDS 55.0%
!! CTACTGGTGCTGCAGTCGAA+GGG - chr3.3:85197742-85197761 None:intergenic 55.0%
!! CTGCAGTCGAAGGGTGTTTG+AGG - chr3.3:85197733-85197752 None:intergenic 55.0%
!! GCTACTGGTGCTGCAGTCGA+AGG - chr3.3:85197743-85197762 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 85197602 85199584 85197602 ID=MS.gene012873
chr3.3 mRNA 85197602 85199584 85197602 ID=MS.gene012873.t1;Parent=MS.gene012873
chr3.3 exon 85197602 85199584 85197602 ID=MS.gene012873.t1.exon1;Parent=MS.gene012873.t1
chr3.3 CDS 85197602 85199584 85197602 ID=cds.MS.gene012873.t1;Parent=MS.gene012873.t1
Gene Sequence

>MS.gene012873

ATGGATTCATGTTCACCACCTCCTGATTTCAACATAGAACCTGAGAACTGCCCAGAACCTAACAATGCCAAAAACTACATTTCTCTTAGAAAACCCTGCGAGAGTTGCATTGAAGAAGGAGGGTGTTCCCTCAAACACCCTTCGACTGCAGCACCAGTAGCAGTAGCAGCAATTTCTTCGTCTCTGTTACGGTTGAAAAAGAGATTGAGAGAATCCGAGGAAACAGCAGAAGCAACAATTTCTTTGTCTCTATCACAGTCGAAAAAGCGACCAAGAGAATCCGAGGAAGCAGCAGCAATAGCAATTTCTTCAGAATCCGATGAATCAACTTCTGATGATGATTCTGAAGTCTCTAGTTGCAGTTCTGAATCAACTTCTGACGAGGATTTTGAAGTCTCGAGTTTCAGTTCGATTAAAAAATCTACGGTTACTACGGTGTCAACATCAATGGAAAATGAGAAGCATGTGACTCGATCTGAACCAGAAGTTACTAATTCGGTGGGTAGTGGTAAAAGAGGAAAGGTTTTAAAAATGAGTACAATGGAGAAGAATTCAGACGATTGTGGTAGAAGAGGGAAAGTATTAAAGATGAGTATAATGGAGAAGAATTCAGAGGTTTACGGTAAGAGAGGGAAGGTATCAAAGACGGAAGGTACAAGGGAAAGGTATTCACTTGTTTCTTGGTTGATAAAGAATCAAGTTTTGGTTTCTGGGACTACTGTGTTTTGTTGTGGTAGTAACAATGTTGTCAAAAGAGGGAGTTTATTTTATGATGGCATAGTTTGTGATTGTTGTCGTGTTAATTTCACTATCACTGGATTTGAGGCTCATGCTGGTTGTACAAGGCATAGACCATCCACTACTATCTTGTTGGAAGACGGAAGGTCATTGTTGGAATGTCAGAGGGAAGCTCAGAGTTCGCATGATGAAAAGGGGAATCATTGTGTGGCTGAAGCAGATTCTGAAGCACAAAATCATTGTGTTTCCGAAGCAAGTTCTGAAGTAGGGAATCACTGTGTTTCCAAAGCAAATAATGATGGTGTTTGTTCTATTTGTGGTTTGGGAGGTGATATAGTACTATGTGATCGATGTCCTTCTGCATTCCACCTCGTTTGCTTAGGTCTCGATCAAGTTCCGGATGGTGATTGGTTTTGTCCAACTTGTTGTTGCAAAATATGTTATCACCCAAAATGCAAACAACAATGTGCAGATGAAAATGATAACAATATTCTTGTTTGTGTTCAATGTGAGCAAAATTTTCACTTTGGATGTGTAAAGGCTACAAGATTTGGATCTAGTCACATGGAGAGCAATGTTAAAAACAGGAACTGGTTTTGCAGTGTAGTTTGCGGGAAGATGTTCTTCTGTCTGAAGAAGCTATTAGGGAAACCAATTAAGGTTGCAAATAAAATAAATTGGACACTGTTGAAGAATTTGAGTAGTGATGATGATGGCGGAGATTTTACTTCAAATGAGCTTAGTCAAAAGAAGAGAAAGTTGAATGTGGCTCTTGATGTATTGTATGAGAGTTTTAACCCAACTATTGATGCTTTATCTGGAAGAGAAATGATTAAAGATGTCGTTTTTAGTAGAGGCTCAAAACATAAACGCTTGAATTTCCATGGTTTCTATAATGTGATTTTAGAGAAAATGGGTAAAGTCATTTCGGTTGCAACTATAAGAATTTATGGTCAAAAGGTTGCCGAGATAGTTTTCGTGGCTACCAAGGAACAATATCGAAGACAAGGAATGTGTCGTTTGTTGATGCACGAGCTTGAGAAACAGTTGACACGCTTAGGAGTTGAAAGCTTGGTTTTGCATTCCTCCGAGGATGCTATAGACACGTGGACAAAAGCGTTTGGTTTTGCTAGGATAACCCGTGAAGACAAGTGTCAATTTGTTGATAAAACTTTCTTAGAATTTCAGAACACTATCATGTGCCTTAAATCCCTAAATAGACCTATATGGCCTTGTATTGCTTGA
Protein sequence

>MS.gene012873.t1

MDSCSPPPDFNIEPENCPEPNNAKNYISLRKPCESCIEEGGCSLKHPSTAAPVAVAAISSSLLRLKKRLRESEETAEATISLSLSQSKKRPRESEEAAAIAISSESDESTSDDDSEVSSCSSESTSDEDFEVSSFSSIKKSTVTTVSTSMENEKHVTRSEPEVTNSVGSGKRGKVLKMSTMEKNSDDCGRRGKVLKMSIMEKNSEVYGKRGKVSKTEGTRERYSLVSWLIKNQVLVSGTTVFCCGSNNVVKRGSLFYDGIVCDCCRVNFTITGFEAHAGCTRHRPSTTILLEDGRSLLECQREAQSSHDEKGNHCVAEADSEAQNHCVSEASSEVGNHCVSKANNDGVCSICGLGGDIVLCDRCPSAFHLVCLGLDQVPDGDWFCPTCCCKICYHPKCKQQCADENDNNILVCVQCEQNFHFGCVKATRFGSSHMESNVKNRNWFCSVVCGKMFFCLKKLLGKPIKVANKINWTLLKNLSSDDDGGDFTSNELSQKKRKLNVALDVLYESFNPTIDALSGREMIKDVVFSRGSKHKRLNFHGFYNVILEKMGKVISVATIRIYGQKVAEIVFVATKEQYRRQGMCRLLMHELEKQLTRLGVESLVLHSSEDAIDTWTKAFGFARITREDKCQFVDKTFLEFQNTIMCLKSLNRPIWPCIA