AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012881

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012881.t1 MTR_3g103030 89.720 321 27 1 1 315 1 321 0.0 600
MS.gene012881.t1 MTR_1g018090 49.176 364 104 12 1 298 1 349 8.85e-86 262
MS.gene012881.t1 MTR_1g018090 45.317 331 100 12 1 265 1 316 3.28e-60 195
MS.gene012881.t1 MTR_5g005110 40.237 338 151 15 1 313 1 312 1.80e-54 181
MS.gene012881.t1 MTR_1g106460 51.445 173 63 4 147 308 161 323 8.35e-47 160
MS.gene012881.t1 MTR_1g106470 78.161 87 19 0 222 308 195 281 2.80e-43 150
MS.gene012881.t1 MTR_1g106480 47.712 153 45 4 187 308 3 151 5.23e-33 119
MS.gene012881.t1 MTR_5g005110 49.630 135 53 5 136 262 136 263 4.79e-23 96.7
MS.gene012881.t1 MTR_5g079500 58.571 70 29 0 222 291 217 286 2.06e-22 95.5
MS.gene012881.t1 MTR_4g131160 52.632 76 36 0 222 297 195 270 2.64e-18 85.1
MS.gene012881.t1 MTR_4g131160 52.632 76 36 0 222 297 198 273 2.74e-18 85.1
MS.gene012881.t1 MTR_8g093500 60.606 66 25 1 222 287 114 178 1.75e-17 81.3
MS.gene012881.t1 MTR_1g080890 46.341 82 44 0 222 303 192 273 4.65e-16 78.2
MS.gene012881.t1 MTR_3g101810 53.968 63 29 0 223 285 171 233 9.07e-16 76.3
MS.gene012881.t1 MTR_7g069590 54.545 55 25 0 230 284 33 87 1.05e-14 70.9
MS.gene012881.t1 MTR_1g106460 38.889 126 56 4 147 261 161 276 5.56e-14 71.2
MS.gene012881.t1 MTR_1g019240 39.241 79 48 0 222 300 84 162 1.11e-13 70.1
MS.gene012881.t1 MTR_1g106470 74.419 43 11 0 222 264 195 237 1.42e-13 69.7
MS.gene012881.t1 MTR_1g084980 45.455 66 36 0 221 286 460 525 4.10e-13 70.5
MS.gene012881.t1 MTR_4g033295 53.030 66 30 1 222 286 117 182 1.22e-12 65.9
MS.gene012881.t1 MTR_4g033295 50.746 67 32 1 222 287 117 183 1.23e-12 65.9
MS.gene012881.t1 MTR_8g062240 31.847 157 88 5 139 286 69 215 3.31e-12 66.2
MS.gene012881.t1 MTR_1g069155 43.939 66 37 0 221 286 315 380 5.52e-12 66.6
MS.gene012881.t1 MTR_4g065870 39.823 113 66 2 175 286 308 419 5.94e-12 66.2
MS.gene012881.t1 MTR_7g099540 36.458 96 56 1 191 286 225 315 6.93e-12 66.2
MS.gene012881.t1 MTR_3g449770 30.822 146 89 2 154 292 233 373 7.39e-12 66.2
MS.gene012881.t1 MTR_1g052470 54.545 66 29 1 222 286 297 362 1.36e-11 65.1
MS.gene012881.t1 MTR_7g053410 30.526 190 116 5 98 286 207 381 1.37e-11 65.5
MS.gene012881.t1 MTR_7g053410 30.526 190 116 5 98 286 207 381 1.48e-11 65.5
MS.gene012881.t1 MTR_8g012290 53.125 64 29 1 224 286 292 355 1.73e-11 65.1
MS.gene012881.t1 MTR_8g012290 53.125 64 29 1 224 286 292 355 1.77e-11 65.1
MS.gene012881.t1 MTR_5g017040 42.188 64 37 0 236 299 118 181 1.81e-11 64.7
MS.gene012881.t1 MTR_1g017350 51.562 64 30 1 224 286 261 324 2.09e-11 64.7
MS.gene012881.t1 MTR_1g017350 51.562 64 30 1 224 286 261 324 2.28e-11 64.7
MS.gene012881.t1 MTR_1g017350 51.562 64 30 1 224 286 261 324 2.44e-11 64.3
MS.gene012881.t1 MTR_1g017350 51.562 64 30 1 224 286 261 324 2.57e-11 64.3
MS.gene012881.t1 MTR_7g110810 46.269 67 34 1 220 286 201 265 2.81e-11 63.9
MS.gene012881.t1 MTR_5g037250 50.000 64 31 1 224 286 113 176 3.38e-11 62.8
MS.gene012881.t1 MTR_4g065870 39.091 110 65 2 175 283 308 416 5.09e-11 63.5
MS.gene012881.t1 MTR_4g070320 51.562 64 30 1 224 286 144 207 5.10e-11 63.2
MS.gene012881.t1 MTR_2g098310 50.000 66 32 1 222 286 331 396 5.93e-11 63.2
MS.gene012881.t1 MTR_3g498825 53.125 64 29 1 224 286 148 211 7.72e-11 62.4
MS.gene012881.t1 MTR_3g116770 51.562 64 30 1 224 286 192 255 8.10e-11 62.8
MS.gene012881.t1 MTR_3g116770 51.562 64 30 1 224 286 192 255 8.51e-11 62.8
MS.gene012881.t1 MTR_5g048860 51.562 64 30 1 224 286 366 429 8.66e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012881.t1 AT2G14760 50.230 217 74 6 109 307 121 321 2.62e-56 185
MS.gene012881.t1 AT4G33880 59.119 159 62 1 150 305 190 348 9.62e-55 182
MS.gene012881.t1 AT1G27740 61.486 148 40 3 162 308 121 252 3.97e-53 175
MS.gene012881.t1 AT2G14760 47.391 230 74 7 109 307 121 334 1.15e-52 176
MS.gene012881.t1 AT5G43175 57.931 145 52 3 165 308 81 217 7.68e-46 155
MS.gene012881.t1 AT5G37800 61.039 77 29 1 216 291 193 269 1.42e-23 98.6
MS.gene012881.t1 AT1G66470 52.809 89 42 0 203 291 175 263 2.76e-23 97.8
MS.gene012881.t1 AT2G24260 51.613 93 43 1 207 297 17 109 1.05e-21 92.4
MS.gene012881.t1 AT4G30980 47.573 103 47 2 189 290 101 197 3.40e-21 92.0
MS.gene012881.t1 AT4G30980 47.573 103 47 2 189 290 149 245 4.52e-21 92.4
MS.gene012881.t1 AT2G24260 51.613 93 43 1 207 297 120 212 6.91e-21 92.0
MS.gene012881.t1 AT5G58010 46.875 96 47 2 222 313 98 193 2.17e-19 87.0
MS.gene012881.t1 AT3G21330 52.381 63 30 0 223 285 269 331 2.65e-15 76.3
MS.gene012881.t1 AT5G50915 34.637 179 77 7 170 314 57 229 7.77e-15 73.9
MS.gene012881.t1 AT5G50915 34.637 179 77 7 170 314 57 229 7.77e-15 73.9
MS.gene012881.t1 AT5G50915 34.637 179 77 7 170 314 57 229 8.93e-15 73.6
MS.gene012881.t1 AT5G50915 34.637 179 77 7 170 314 57 229 8.93e-15 73.6
MS.gene012881.t1 AT5G01305 49.180 61 31 0 222 282 36 96 1.30e-14 71.2
MS.gene012881.t1 AT4G00120 50.000 54 27 0 232 285 121 174 3.46e-14 70.5
MS.gene012881.t1 AT1G25330 50.617 81 39 1 224 303 105 185 4.24e-13 67.8
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT1G09530 37.895 95 53 1 201 295 321 409 6.04e-13 69.7
MS.gene012881.t1 AT4G00050 39.423 104 51 3 183 286 77 168 9.80e-13 67.8
MS.gene012881.t1 AT1G51140 53.846 65 29 1 222 285 303 367 1.14e-12 68.6
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 185 303 1.37e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 185 303 1.37e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 185 303 1.37e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 256 374 1.52e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 256 374 1.52e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 256 374 1.52e-12 68.2
MS.gene012881.t1 AT2G20180 32.031 128 69 3 196 314 256 374 1.52e-12 68.2
MS.gene012881.t1 AT1G18400 54.688 64 28 1 224 286 146 209 1.67e-12 66.6
MS.gene012881.t1 AT1G18400 54.688 64 28 1 224 286 146 209 2.15e-12 66.6
MS.gene012881.t1 AT4G00050 39.423 104 51 3 183 286 179 270 2.20e-12 67.8
MS.gene012881.t1 AT4G00050 39.423 104 51 3 183 286 179 270 2.43e-12 67.4
MS.gene012881.t1 AT2G46970 30.645 124 80 2 195 313 165 287 4.16e-12 66.6
MS.gene012881.t1 AT4G09180 48.529 68 34 1 222 288 183 250 4.58e-12 65.5
MS.gene012881.t1 AT2G46970 30.645 124 80 2 195 313 196 318 4.63e-12 66.6
MS.gene012881.t1 AT2G46970 30.645 124 80 2 195 313 174 296 4.73e-12 66.6
MS.gene012881.t1 AT1G35460 50.000 66 32 1 222 286 180 245 8.73e-12 64.7
MS.gene012881.t1 AT4G28790 45.946 74 39 1 227 300 275 347 1.16e-11 65.5
MS.gene012881.t1 AT1G73830 51.562 64 30 1 224 286 148 211 1.23e-11 64.3
MS.gene012881.t1 AT1G73830 51.562 64 30 1 224 286 148 211 1.24e-11 64.3
MS.gene012881.t1 AT4G28815 38.776 98 55 2 206 298 47 144 1.28e-11 63.9
MS.gene012881.t1 AT4G28815 38.776 98 55 2 206 298 123 220 1.98e-11 64.3
MS.gene012881.t1 AT2G18300 36.434 129 73 3 159 286 129 249 2.17e-11 64.3
MS.gene012881.t1 AT2G18300 36.434 129 73 3 159 286 129 249 2.29e-11 64.3
MS.gene012881.t1 AT1G68920 32.237 152 101 2 136 286 216 366 2.33e-11 64.7
MS.gene012881.t1 AT2G18300 36.434 129 73 3 159 286 129 249 2.41e-11 64.3
MS.gene012881.t1 AT5G67110 40.404 99 45 3 219 315 81 167 2.42e-11 62.8
MS.gene012881.t1 AT3G07340 53.125 64 29 1 224 286 259 322 2.88e-11 64.3
MS.gene012881.t1 AT3G07340 53.125 64 29 1 224 286 259 322 3.08e-11 64.3
MS.gene012881.t1 AT4G36930 31.034 116 79 1 200 314 167 282 3.28e-11 63.9
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 173 236 3.81e-11 63.5
MS.gene012881.t1 AT2G42280 52.308 65 30 1 222 285 278 342 4.04e-11 63.5
MS.gene012881.t1 AT5G48560 53.125 64 29 1 224 286 302 365 4.07e-11 63.9
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 224 287 4.23e-11 63.5
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 224 287 4.23e-11 63.5
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 224 287 4.23e-11 63.5
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 224 287 4.23e-11 63.5
MS.gene012881.t1 AT5G48560 53.125 64 29 1 224 286 302 365 4.46e-11 63.9
MS.gene012881.t1 AT1G26260 53.125 64 29 1 224 286 202 265 4.65e-11 63.5
MS.gene012881.t1 AT1G59640 50.000 64 31 1 224 286 137 200 4.91e-11 62.8
MS.gene012881.t1 AT1G10120 51.562 64 30 1 224 286 207 270 4.98e-11 63.5
MS.gene012881.t1 AT1G10120 51.562 64 30 1 224 286 207 270 4.98e-11 63.5
MS.gene012881.t1 AT2G20180 37.079 89 51 1 196 284 256 339 5.75e-11 63.2
MS.gene012881.t1 AT4G34530 51.562 64 30 1 224 286 173 236 7.48e-11 62.8
MS.gene012881.t1 AT3G23690 50.000 64 31 1 224 286 192 255 8.95e-11 62.8
MS.gene012881.t1 AT1G68920 51.562 64 30 1 224 286 304 367 9.93e-11 62.8
MS.gene012881.t1 AT1G68920 51.562 64 30 1 224 286 304 367 9.93e-11 62.8
MS.gene012881.t1 AT1G68920 51.562 64 30 1 224 286 304 367 9.93e-11 62.8

Find 58 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCTTCTTGCTCTTCT+TGG 0.125181 3.3:-85067307 None:intergenic
TGAGTATTATGATTACTTTC+TGG 0.228641 3.3:-85066853 None:intergenic
GCTTGATGTGCCAGAATTTC+AGG 0.228928 3.3:+85066990 MS.gene012881:CDS
GCTGCAGAAATTTCACATAT+TGG 0.264015 3.3:-85067955 None:intergenic
ACTCATATATTACCTTGATT+TGG 0.291701 3.3:-85067979 None:intergenic
TCTACATGCACCTGAAATTC+TGG 0.295024 3.3:-85067000 None:intergenic
TAAATTACCTTTGTCCCATT+TGG 0.321779 3.3:-85067809 None:intergenic
TCAAGTCCATTATAAGCAAT+TGG 0.332783 3.3:-85068145 None:intergenic
TATGCCCCAATTGCTTATAA+TGG 0.346115 3.3:+85068139 MS.gene012881:CDS
CTTCTGCTTGGAGAAAATAA+TGG 0.350452 3.3:+85066727 MS.gene012881:CDS
CAAGTCCATTATAAGCAATT+GGG 0.382500 3.3:-85068144 None:intergenic
CTACAAAATCTTGTACCAAA+TGG 0.385534 3.3:+85067794 MS.gene012881:CDS
CATTCCCATTCGCCATCATC+AGG 0.393250 3.3:-85066637 None:intergenic
ACCCTTGCATAGAGGCTTTG+AGG 0.393741 3.3:-85067513 None:intergenic
TACAAAATCTTGTACCAAAT+GGG 0.416760 3.3:+85067795 MS.gene012881:CDS
GGAGAAAATAATGGGAACTT+TGG 0.422902 3.3:+85066736 MS.gene012881:CDS
GAAGCCAATGCAAGTGTAGA+TGG 0.429354 3.3:+85067351 MS.gene012881:CDS
TTCTGCTTGGAGAAAATAAT+GGG 0.435352 3.3:+85066728 MS.gene012881:CDS
AACCTAAGTTGAAGCTGAAA+AGG 0.448825 3.3:+85066965 MS.gene012881:CDS
ACATTGGATTCAATCTCAAC+CGG 0.458816 3.3:+85068167 MS.gene012881:CDS
TTGCTCCCCTTCTGAAGCTG+AGG 0.486357 3.3:+85066774 MS.gene012881:CDS
GATCAAACCTCGCTTCTGCT+TGG 0.495958 3.3:+85066715 MS.gene012881:CDS
GCTTATAATGGACTTGACAT+TGG 0.507048 3.3:+85068151 MS.gene012881:CDS
ATTTCTGCAGCTCCAAATCA+AGG 0.513225 3.3:+85067967 MS.gene012881:CDS
TATCCCTGATGATGGCGAAT+GGG 0.522217 3.3:+85066633 MS.gene012881:CDS
ATGGTGGTGGAGAAATCTTC+CGG 0.539717 3.3:-85068186 None:intergenic
ATCCTCAAAGCCTCTATGCA+AGG 0.540493 3.3:+85067511 MS.gene012881:CDS
ATGTTTCCCTCAGCTTCAGA+AGG 0.542520 3.3:-85066781 None:intergenic
CTTGGAGCTATCCCTGATGA+TGG 0.545545 3.3:+85066625 MS.gene012881:CDS
TGCTCCCCTTCTGAAGCTGA+GGG 0.548801 3.3:+85066775 MS.gene012881:CDS
AAGATAAGATAAACTCATGT+GGG 0.556515 3.3:+85067022 MS.gene012881:CDS
ACTGTGTCTCTCAACTCCAA+TGG 0.558276 3.3:+85067456 MS.gene012881:CDS
ATGTCCATCTACACTTGCAT+TGG 0.565311 3.3:-85067355 None:intergenic
TGATAATTACCCTTGCATAG+AGG 0.574539 3.3:-85067521 None:intergenic
TGTTTCCCTCAGCTTCAGAA+GGG 0.575825 3.3:-85066780 None:intergenic
CTATCCCTGATGATGGCGAA+TGG 0.588808 3.3:+85066632 MS.gene012881:CDS
GAAGATAAGATAAACTCATG+TGG 0.590178 3.3:+85067021 MS.gene012881:CDS
ATTGTGGTGGAGAGTCATCA+TGG 0.590276 3.3:-85066687 None:intergenic
TCCTCAAAGCCTCTATGCAA+GGG 0.593714 3.3:+85067512 MS.gene012881:CDS
TTCAGAAGGGGAGCAAAACA+TGG 0.599755 3.3:-85066767 None:intergenic
TCTTGTACCAAATGGGACAA+AGG 0.600952 3.3:+85067802 MS.gene012881:CDS
CTCTGAGGTATAACCACTAG+AGG 0.615332 3.3:-85067379 None:intergenic
GAAAGTAATCATAATACTCA+AGG 0.616654 3.3:+85066856 MS.gene012881:CDS
AAGTCCATTATAAGCAATTG+GGG 0.617408 3.3:-85068143 None:intergenic
GTTTCCCTCAGCTTCAGAAG+GGG 0.626059 3.3:-85066779 None:intergenic
GGAAAGAAAAGAGCTAGTAG+AGG 0.626774 3.3:+85067477 MS.gene012881:CDS
CTCTAGTGGTTATACCTCAG+AGG 0.631424 3.3:+85067380 MS.gene012881:CDS
TTCTAAGCTCTGATGATCTG+TGG 0.632585 3.3:+85068113 MS.gene012881:CDS
GAGGTTTGATCAAGTAATTG+TGG 0.634212 3.3:-85066703 None:intergenic
AGAGTCCAAGAAGAGCAAGA+AGG 0.635660 3.3:+85067302 MS.gene012881:CDS
GTTTGATCAAGTAATTGTGG+TGG 0.639569 3.3:-85066700 None:intergenic
ATTCCCATTCGCCATCATCA+GGG 0.639610 3.3:-85066636 None:intergenic
GTAGATGGACATACCTCTAG+TGG 0.644629 3.3:+85067366 MS.gene012881:CDS
CTTCAGTTGCAAATATTCTG+CGG 0.648235 3.3:-85066663 None:intergenic
GGTTGATAGAAATGAAAACA+AGG 0.650583 3.3:+85067323 MS.gene012881:CDS
AGAGGAGCAAAACATGTGTG+AGG 0.671006 3.3:+85067398 MS.gene012881:CDS
ATGTGTGAGGAAAACAGTGG+AGG 0.712518 3.3:+85067411 MS.gene012881:CDS
AACATGTGTGAGGAAAACAG+TGG 0.786485 3.3:+85067408 MS.gene012881:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATTATTACTAATTAAAG+TGG - chr3.3:85067232-85067251 None:intergenic 15.0%
!!! AATAATGATTTGAAATTTTG+TGG + chr3.3:85067243-85067262 MS.gene012881:intron 15.0%
!! ATTTCTGTTTAGAAATATCA+AGG + chr3.3:85068028-85068047 MS.gene012881:intron 20.0%
!! GAGATGTATAATTTGATATA+TGG + chr3.3:85067886-85067905 MS.gene012881:intron 20.0%
!! TGTATAATTTGATATATGGA+TGG + chr3.3:85067890-85067909 MS.gene012881:intron 20.0%
!!! AATGTTTTGCATTTTAAAGA+GGG + chr3.3:85067842-85067861 MS.gene012881:intron 20.0%
!!! TTGAATTTTACAGAAAAGAA+GGG + chr3.3:85067745-85067764 MS.gene012881:intron 20.0%
!!! TTTGAATTTTACAGAAAAGA+AGG + chr3.3:85067744-85067763 MS.gene012881:intron 20.0%
! AAGAGGAATAGTTAAACAAA+AGG - chr3.3:85067567-85067586 None:intergenic 25.0%
! AAGATAAGATAAACTCATGT+GGG + chr3.3:85067022-85067041 MS.gene012881:CDS 25.0%
! ACTCATATATTACCTTGATT+TGG - chr3.3:85067982-85068001 None:intergenic 25.0%
! GAAAGTAATCATAATACTCA+AGG + chr3.3:85066856-85066875 MS.gene012881:CDS 25.0%
! TACAAAATCTTGTACCAAAT+GGG + chr3.3:85067795-85067814 MS.gene012881:CDS 25.0%
! TCATATGATATTTACTCCAT+CGG + chr3.3:85067104-85067123 MS.gene012881:intron 25.0%
! TGAGTATTATGATTACTTTC+TGG - chr3.3:85066856-85066875 None:intergenic 25.0%
!! TTGACATTTTGTAATCTCAA+AGG + chr3.3:85067159-85067178 MS.gene012881:intron 25.0%
!!! GAATGTTTTGCATTTTAAAG+AGG + chr3.3:85067841-85067860 MS.gene012881:intron 25.0%
!!! TAATTAATTTGTCAGCTTAG+CGG + chr3.3:85067128-85067147 MS.gene012881:intron 25.0%
AAGTCCATTATAAGCAATTG+GGG - chr3.3:85068146-85068165 None:intergenic 30.0%
ACAGAATGGAGATTAAAAAG+AGG - chr3.3:85067584-85067603 None:intergenic 30.0%
ACTAATTAAAGTGGTACTAG+TGG - chr3.3:85067223-85067242 None:intergenic 30.0%
CAAGTCCATTATAAGCAATT+GGG - chr3.3:85068147-85068166 None:intergenic 30.0%
CTACAAAATCTTGTACCAAA+TGG + chr3.3:85067794-85067813 MS.gene012881:CDS 30.0%
CTGACAAATTAATTAACCGA+TGG - chr3.3:85067123-85067142 None:intergenic 30.0%
GAAGATAAGATAAACTCATG+TGG + chr3.3:85067021-85067040 MS.gene012881:CDS 30.0%
TAAATTACCTTTGTCCCATT+TGG - chr3.3:85067812-85067831 None:intergenic 30.0%
TCAAGTCCATTATAAGCAAT+TGG - chr3.3:85068148-85068167 None:intergenic 30.0%
TGCTATATACTTCATTCACT+TGG - chr3.3:85067701-85067720 None:intergenic 30.0%
TTCTGCTTGGAGAAAATAAT+GGG + chr3.3:85066728-85066747 MS.gene012881:CDS 30.0%
! ATTTTGTGGTTTCCAAATTC+AGG + chr3.3:85067257-85067276 MS.gene012881:intron 30.0%
! GGGACAAAGGTAATTTATTT+TGG + chr3.3:85067815-85067834 MS.gene012881:intron 30.0%
!! GGTTGATAGAAATGAAAACA+AGG + chr3.3:85067323-85067342 MS.gene012881:CDS 30.0%
!! TTTTGATTGTTCTGATCATG+TGG + chr3.3:85066894-85066913 MS.gene012881:CDS 30.0%
ACATTGGATTCAATCTCAAC+CGG + chr3.3:85068167-85068186 MS.gene012881:CDS 35.0%
ATATATGGATGGTCATACAC+AGG + chr3.3:85067901-85067920 MS.gene012881:intron 35.0%
CATATTACAATCGTCTCTCA+TGG - chr3.3:85067199-85067218 None:intergenic 35.0%
CTTCAGTTGCAAATATTCTG+CGG - chr3.3:85066666-85066685 None:intergenic 35.0%
CTTCTGCTTGGAGAAAATAA+TGG + chr3.3:85066727-85066746 MS.gene012881:CDS 35.0%
GAGGTTTGATCAAGTAATTG+TGG - chr3.3:85066706-85066725 None:intergenic 35.0%
GCTGCAGAAATTTCACATAT+TGG - chr3.3:85067958-85067977 None:intergenic 35.0%
GTTTGATCAAGTAATTGTGG+TGG - chr3.3:85066703-85066722 None:intergenic 35.0%
TATGCCCCAATTGCTTATAA+TGG + chr3.3:85068139-85068158 MS.gene012881:CDS 35.0%
TCCTTCCAAAGAAGAAAAGA+AGG - chr3.3:85067623-85067642 None:intergenic 35.0%
TGATAATTACCCTTGCATAG+AGG - chr3.3:85067524-85067543 None:intergenic 35.0%
! AACCTAAGTTGAAGCTGAAA+AGG + chr3.3:85066965-85066984 MS.gene012881:CDS 35.0%
! ATCCTTTTCAGCTTCAACTT+AGG - chr3.3:85066970-85066989 None:intergenic 35.0%
! GGAGAAAATAATGGGAACTT+TGG + chr3.3:85066736-85066755 MS.gene012881:CDS 35.0%
! TTTTCAGCTTCAACTTAGGT+TGG - chr3.3:85066966-85066985 None:intergenic 35.0%
!! ACTAGCTCTTTTCTTTCCAT+TGG - chr3.3:85067475-85067494 None:intergenic 35.0%
!! GCTTATAATGGACTTGACAT+TGG + chr3.3:85068151-85068170 MS.gene012881:CDS 35.0%
!! TCTTTTCTTCTTTGGAAGGA+TGG + chr3.3:85067623-85067642 MS.gene012881:intron 35.0%
AACATGTGTGAGGAAAACAG+TGG + chr3.3:85067408-85067427 MS.gene012881:CDS 40.0%
ACATGCTATGGTTCACAGAA+TGG - chr3.3:85067598-85067617 None:intergenic 40.0%
ATCAACCTTCTTGCTCTTCT+TGG - chr3.3:85067310-85067329 None:intergenic 40.0%
ATGTCCATCTACACTTGCAT+TGG - chr3.3:85067358-85067377 None:intergenic 40.0%
ATTCTGTGAACCATAGCATG+TGG + chr3.3:85067597-85067616 MS.gene012881:intron 40.0%
ATTTCTGCAGCTCCAAATCA+AGG + chr3.3:85067967-85067986 MS.gene012881:CDS 40.0%
GGAAAGAAAAGAGCTAGTAG+AGG + chr3.3:85067477-85067496 MS.gene012881:CDS 40.0%
TCTACATGCACCTGAAATTC+TGG - chr3.3:85067003-85067022 None:intergenic 40.0%
TCTTGTACCAAATGGGACAA+AGG + chr3.3:85067802-85067821 MS.gene012881:CDS 40.0%
TGCATCTTTGCACCTGAATT+TGG - chr3.3:85067272-85067291 None:intergenic 40.0%
! GCCTTCTTTTCTTCTTTGGA+AGG + chr3.3:85067619-85067638 MS.gene012881:intron 40.0%
! TGTGGCCTTCTTTTCTTCTT+TGG + chr3.3:85067615-85067634 MS.gene012881:intron 40.0%
! TTCTAAGCTCTGATGATCTG+TGG + chr3.3:85068113-85068132 MS.gene012881:CDS 40.0%
!!! CAGCACGAGGTTTTTTCTTA+GGG - chr3.3:85067050-85067069 None:intergenic 40.0%
ACTGTGTCTCTCAACTCCAA+TGG + chr3.3:85067456-85067475 MS.gene012881:CDS 45.0%
AGAGGAGCAAAACATGTGTG+AGG + chr3.3:85067398-85067417 MS.gene012881:CDS 45.0%
AGAGTCCAAGAAGAGCAAGA+AGG + chr3.3:85067302-85067321 MS.gene012881:CDS 45.0%
ATCCTCAAAGCCTCTATGCA+AGG + chr3.3:85067511-85067530 MS.gene012881:CDS 45.0%
ATGTGTGAGGAAAACAGTGG+AGG + chr3.3:85067411-85067430 MS.gene012881:CDS 45.0%
ATGTTTCCCTCAGCTTCAGA+AGG - chr3.3:85066784-85066803 None:intergenic 45.0%
ATTCCCATTCGCCATCATCA+GGG - chr3.3:85066639-85066658 None:intergenic 45.0%
CTCTAGTGGTTATACCTCAG+AGG + chr3.3:85067380-85067399 MS.gene012881:CDS 45.0%
GAAAAGAAGGCCACATGCTA+TGG - chr3.3:85067610-85067629 None:intergenic 45.0%
GAAGCCAATGCAAGTGTAGA+TGG + chr3.3:85067351-85067370 MS.gene012881:CDS 45.0%
GCTTGATGTGCCAGAATTTC+AGG + chr3.3:85066990-85067009 MS.gene012881:CDS 45.0%
GTAGATGGACATACCTCTAG+TGG + chr3.3:85067366-85067385 MS.gene012881:CDS 45.0%
TCCTCAAAGCCTCTATGCAA+GGG + chr3.3:85067512-85067531 MS.gene012881:CDS 45.0%
TGTTTCCCTCAGCTTCAGAA+GGG - chr3.3:85066783-85066802 None:intergenic 45.0%
TTCAGAAGGGGAGCAAAACA+TGG - chr3.3:85066770-85066789 None:intergenic 45.0%
! ACACATGTTTTGCTCCTCTG+AGG - chr3.3:85067397-85067416 None:intergenic 45.0%
! ATTGTGGTGGAGAGTCATCA+TGG - chr3.3:85066690-85066709 None:intergenic 45.0%
! ATTTTCTCCAAGCAGAAGCG+AGG - chr3.3:85066725-85066744 None:intergenic 45.0%
! CTTACATCTTTTGCAGCACG+AGG - chr3.3:85067063-85067082 None:intergenic 45.0%
!! ATGGTGGTGGAGAAATCTTC+CGG - chr3.3:85068189-85068208 None:intergenic 45.0%
!! CTCTGAGGTATAACCACTAG+AGG - chr3.3:85067382-85067401 None:intergenic 45.0%
!! TATCCCTGATGATGGCGAAT+GGG + chr3.3:85066633-85066652 MS.gene012881:CDS 45.0%
!!! GCAGCACGAGGTTTTTTCTT+AGG - chr3.3:85067051-85067070 None:intergenic 45.0%
AATCTCGCCATGCCATCAAG+GGG - chr3.3:85067658-85067677 None:intergenic 50.0%
ACCCTTGCATAGAGGCTTTG+AGG - chr3.3:85067516-85067535 None:intergenic 50.0%
CATTCCCATTCGCCATCATC+AGG - chr3.3:85066640-85066659 None:intergenic 50.0%
CTTGGAGCTATCCCTGATGA+TGG + chr3.3:85066625-85066644 MS.gene012881:CDS 50.0%
GAATCTCGCCATGCCATCAA+GGG - chr3.3:85067659-85067678 None:intergenic 50.0%
GATCAAACCTCGCTTCTGCT+TGG + chr3.3:85066715-85066734 MS.gene012881:CDS 50.0%
GTTTCCCTCAGCTTCAGAAG+GGG - chr3.3:85066782-85066801 None:intergenic 50.0%
!! CTATCCCTGATGATGGCGAA+TGG + chr3.3:85066632-85066651 MS.gene012881:CDS 50.0%
CGAATCTCGCCATGCCATCA+AGG - chr3.3:85067660-85067679 None:intergenic 55.0%
TGGTCACACTAGCCCCTTGA+TGG + chr3.3:85067643-85067662 MS.gene012881:intron 55.0%
! ACACTAGCCCCTTGATGGCA+TGG + chr3.3:85067648-85067667 MS.gene012881:intron 55.0%
!! TGCTCCCCTTCTGAAGCTGA+GGG + chr3.3:85066775-85066794 MS.gene012881:CDS 55.0%
!! TTGCTCCCCTTCTGAAGCTG+AGG + chr3.3:85066774-85066793 MS.gene012881:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 85066616 85068210 85066616 ID=MS.gene012881
chr3.3 mRNA 85066616 85068210 85066616 ID=MS.gene012881.t1;Parent=MS.gene012881
chr3.3 exon 85066616 85067077 85066616 ID=MS.gene012881.t1.exon1;Parent=MS.gene012881.t1
chr3.3 CDS 85066616 85067077 85066616 ID=cds.MS.gene012881.t1;Parent=MS.gene012881.t1
chr3.3 exon 85067279 85067533 85067279 ID=MS.gene012881.t1.exon2;Parent=MS.gene012881.t1
chr3.3 CDS 85067279 85067533 85067279 ID=cds.MS.gene012881.t1;Parent=MS.gene012881.t1
chr3.3 exon 85067758 85067823 85067758 ID=MS.gene012881.t1.exon3;Parent=MS.gene012881.t1
chr3.3 CDS 85067758 85067823 85067758 ID=cds.MS.gene012881.t1;Parent=MS.gene012881.t1
chr3.3 exon 85067923 85067988 85067923 ID=MS.gene012881.t1.exon4;Parent=MS.gene012881.t1
chr3.3 CDS 85067923 85067988 85067923 ID=cds.MS.gene012881.t1;Parent=MS.gene012881.t1
chr3.3 exon 85068112 85068210 85068112 ID=MS.gene012881.t1.exon5;Parent=MS.gene012881.t1
chr3.3 CDS 85068112 85068210 85068112 ID=cds.MS.gene012881.t1;Parent=MS.gene012881.t1
Gene Sequence

>MS.gene012881

ATGGAAACACTTGGAGCTATCCCTGATGATGGCGAATGGGAATGCTTCCGCAGAATATTTGCAACTGAAGACCATGATGACTCTCCACCACAATTACTTGATCAAACCTCGCTTCTGCTTGGAGAAAATAATGGGAACTTTGGAGTACAATCCATGTTTTGCTCCCCTTCTGAAGCTGAGGGAAACATGAATACTAATGTGTTTTATTCTTTTGATTCTCACATTAACTATAATTCACCAGAAAGTAATCATAATACTCAAGGTCACACAAACTACTATTTTGATTGTTCTGATCATGTGGTAGCTAATAATAGTGACAGAACAGAGAATTTTGATCACAATCAAGTCCAACCTAAGTTGAAGCTGAAAAGGATGCTTGATGTGCCAGAATTTCAGGTGCATGTAGAAGATAAGATAAACTCATGTGGGAACCCTAAGAAAAAACCTCGTGCTGCAAAAGATGTAAGCTTAATTTGTCTATATTTATATCATATGATATTTACTCCATCGGTTAATTAATTTGTCAGCTTAGCGGTTAGAGTCTTGACATTTTGTAATCTCAAAGGACAAAGTTCAAATTCCATGAGAGACGATTGTAATATGACCACTAGTACCACTTTAATTAGTAATAATGATTTGAAATTTTGTGGTTTCCAAATTCAGGTGCAAAGATGCATGAAAAATTCAGAGTCCAAGAAGAGCAAGAAGGTTGATAGAAATGAAAACAAGGCAGAAGAAGCCAATGCAAGTGTAGATGGACATACCTCTAGTGGTTATACCTCAGAGGAGCAAAACATGTGTGAGGAAAACAGTGGAGGAGCTTCTGCAGCATCTAAATCAACTGTGTCTCTCAACTCCAATGGAAAGAAAAGAGCTAGTAGAGGATCAGCAACAGATCCTCAAAGCCTCTATGCAAGGGTAATTATCAATTTCTTTTAAGTACAAATGCCTTTTGTTTAACTATTCCTCTTTTTAATCTCCATTCTGTGAACCATAGCATGTGGCCTTCTTTTCTTCTTTGGAAGGATGGTCACACTAGCCCCTTGATGGCATGGCGAGATTCGCGAAGCGAAGATCATTCGCCAAGTGAATGAAGTATATAGCAATTTATGTGATGCTTATTGTCTTTTTGAATTTTACAGAAAAGAAGGGAGAAAATAAACGAGAGACTGAGAACTCTACAAAATCTTGTACCAAATGGGACAAAGGTAATTTATTTTGGCATGAATGTTTTGCATTTTAAAGAGGGTTGTAAGTTGCAATTATTTGAGAGATGTATAATTTGATATATGGATGGTCATACACAGGTTGATATAAGCACAATGCTTGAAGATGCAATCCAATATGTGAAATTTCTGCAGCTCCAAATCAAGGTAATATATGAGTCGCGAAATATAGACGATTAATAATTAATTTCTGTTTAGAAATATCAAGGTGTGTGTATGTGTTCAATCAGAATAACATCTTTTTAATATGAATGTTTATCTCTTATATAGCTTCTAAGCTCTGATGATCTGTGGATGTATGCCCCAATTGCTTATAATGGACTTGACATTGGATTCAATCTCAACCGGAAGATTTCTCCACCACCATGA
Protein sequence

>MS.gene012881.t1

METLGAIPDDGEWECFRRIFATEDHDDSPPQLLDQTSLLLGENNGNFGVQSMFCSPSEAEGNMNTNVFYSFDSHINYNSPESNHNTQGHTNYYFDCSDHVVANNSDRTENFDHNQVQPKLKLKRMLDVPEFQVHVEDKINSCGNPKKKPRAAKDVQRCMKNSESKKSKKVDRNENKAEEANASVDGHTSSGYTSEEQNMCEENSGGASAASKSTVSLNSNGKKRASRGSATDPQSLYARKRREKINERLRTLQNLVPNGTKVDISTMLEDAIQYVKFLQLQIKLLSSDDLWMYAPIAYNGLDIGFNLNRKISPPP