AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012913

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012913.t1 MTR_3g102600 91.848 184 15 0 1 184 1 184 5.72e-118 332
MS.gene012913.t1 MTR_3g102600 92.265 181 14 0 1 181 1 181 2.63e-115 327
MS.gene012913.t1 MTR_3g102600 93.333 135 9 0 50 184 1 135 2.33e-90 260
MS.gene012913.t1 MTR_3g102600 93.939 132 8 0 50 181 1 132 7.13e-88 255
MS.gene012913.t1 MTR_3g102590 70.769 195 46 2 1 184 1 195 1.95e-85 250
MS.gene012913.t1 MTR_3g102590 70.833 192 45 2 1 181 1 192 2.46e-83 246
MS.gene012913.t1 MTR_3g106220 83.459 133 22 0 1 133 1 133 1.26e-73 233
MS.gene012913.t1 MTR_3g106220 75.000 48 12 0 134 181 200 247 2.40e-19 85.1
MS.gene012913.t1 MTR_2g034960 43.830 235 74 3 5 181 26 260 3.88e-60 197
MS.gene012913.t1 MTR_3g086100 44.492 236 69 2 8 181 13 248 8.66e-60 195
MS.gene012913.t1 MTR_8g079940 44.915 236 70 2 6 181 23 258 3.36e-59 193
MS.gene012913.t1 MTR_2g450070 67.424 132 43 0 2 133 12 143 1.39e-58 194
MS.gene012913.t1 MTR_2g450070 68.750 48 15 0 134 181 215 262 2.83e-16 76.3
MS.gene012913.t1 MTR_2g450070 67.424 132 43 0 2 133 12 143 1.85e-58 194
MS.gene012913.t1 MTR_2g450070 68.750 48 15 0 134 181 215 262 2.85e-16 76.3
MS.gene012913.t1 MTR_1g013170 68.992 129 40 0 5 133 2 130 9.36e-57 186
MS.gene012913.t1 MTR_1g013170 84.783 46 7 0 136 181 195 240 6.40e-19 83.6
MS.gene012913.t1 MTR_1g013180 63.910 133 48 0 1 133 149 281 8.88e-53 179
MS.gene012913.t1 MTR_1g013180 68.750 48 15 0 134 181 344 391 6.92e-15 72.4
MS.gene012913.t1 MTR_7g117705 65.385 130 44 1 5 133 2 131 1.83e-51 171
MS.gene012913.t1 MTR_7g117705 66.000 50 17 0 132 181 191 240 2.20e-16 76.3
MS.gene012913.t1 MTR_4g121020 57.143 133 57 0 3 135 26 158 1.19e-50 173
MS.gene012913.t1 MTR_4g121020 70.833 48 14 0 134 181 217 264 4.47e-17 78.6
MS.gene012913.t1 MTR_4g121020 57.143 133 57 0 3 135 26 158 1.20e-50 173
MS.gene012913.t1 MTR_4g121020 70.833 48 14 0 134 181 217 264 4.47e-17 78.6
MS.gene012913.t1 MTR_4g131580 60.938 128 50 0 2 129 11 138 7.53e-49 167
MS.gene012913.t1 MTR_4g131580 68.750 48 15 0 134 181 202 249 3.32e-15 73.2
MS.gene012913.t1 MTR_4g131600 63.934 122 44 0 8 129 20 141 4.34e-48 165
MS.gene012913.t1 MTR_4g131600 66.667 48 16 0 134 181 203 250 2.36e-15 73.6
MS.gene012913.t1 MTR_4g131570 63.934 122 44 0 8 129 17 138 1.88e-47 163
MS.gene012913.t1 MTR_4g131570 66.667 48 16 0 134 181 205 252 1.73e-14 70.9
MS.gene012913.t1 MTR_1g032570 59.524 126 51 0 4 129 7 132 1.41e-44 155
MS.gene012913.t1 MTR_1g032570 68.750 48 15 0 134 181 202 249 1.75e-16 76.6
MS.gene012913.t1 MTR_4g021760 36.449 214 100 3 3 181 6 218 4.30e-40 138
MS.gene012913.t1 MTR_3g086100 42.938 177 39 2 67 181 1 177 3.09e-38 137
MS.gene012913.t1 MTR_4g021845 34.434 212 103 3 8 184 10 220 7.88e-37 127
MS.gene012913.t1 MTR_4g021790 35.407 209 99 3 8 181 10 217 4.50e-36 127
MS.gene012913.t1 MTR_4g021855 33.493 209 103 2 8 181 10 217 1.12e-34 124
MS.gene012913.t1 MTR_6g045327 34.434 212 103 4 7 183 13 223 5.82e-33 121
MS.gene012913.t1 MTR_0450s0040 32.701 211 101 2 12 181 8 218 7.01e-33 120
MS.gene012913.t1 MTR_7g026400 34.197 193 81 2 35 181 14 206 2.31e-30 116
MS.gene012913.t1 MTR_1g013160 68.750 64 20 0 70 133 1 64 7.69e-25 94.7
MS.gene012913.t1 MTR_1g032570 74.603 63 16 0 67 129 1 63 8.19e-25 100
MS.gene012913.t1 MTR_1g032570 68.750 48 15 0 134 181 133 180 1.43e-16 77.0
MS.gene012913.t1 MTR_5g055260 31.217 189 107 5 8 181 10 190 1.80e-22 91.3
MS.gene012913.t1 MTR_1g067110 38.843 121 71 2 12 129 85 205 8.22e-22 92.0
MS.gene012913.t1 MTR_1g067110 38.843 121 71 2 12 129 85 205 8.26e-22 92.0
MS.gene012913.t1 MTR_4g061360 40.496 121 69 1 12 129 89 209 1.40e-21 91.7
MS.gene012913.t1 MTR_3g092780 37.302 126 74 2 12 133 56 180 4.60e-21 90.1
MS.gene012913.t1 MTR_3g092780 37.302 126 74 2 12 133 56 180 4.67e-21 90.1
MS.gene012913.t1 MTR_7g118260 36.806 144 79 4 1 135 1 141 8.33e-21 89.4
MS.gene012913.t1 MTR_3g092780 37.705 122 71 2 12 129 56 176 8.78e-21 89.4
MS.gene012913.t1 MTR_3g092780 37.705 122 71 2 12 129 56 176 8.91e-21 89.4
MS.gene012913.t1 MTR_7g118260 36.170 141 79 3 1 133 1 138 9.82e-21 89.0
MS.gene012913.t1 MTR_8g093040 34.559 136 84 1 7 142 13 143 2.25e-20 85.9
MS.gene012913.t1 MTR_8g024260 39.496 119 69 1 14 129 38 156 5.05e-20 87.0
MS.gene012913.t1 MTR_6g016850 33.333 126 81 3 6 129 7 131 3.30e-19 80.5
MS.gene012913.t1 MTR_4g108880 33.884 121 77 1 12 129 33 153 7.23e-18 80.9
MS.gene012913.t1 MTR_8g105600 34.146 123 79 2 8 129 9 130 1.44e-17 80.1
MS.gene012913.t1 MTR_8g105600 58.696 46 19 0 136 181 191 236 8.58e-11 60.5
MS.gene012913.t1 MTR_4g121020 70.833 48 14 0 134 181 113 160 2.44e-17 79.3
MS.gene012913.t1 MTR_4g121020 48.980 49 25 0 87 135 6 54 2.11e-11 62.0
MS.gene012913.t1 MTR_3g037390 33.058 121 78 1 12 129 24 144 6.36e-17 78.2
MS.gene012913.t1 MTR_8g032710 32.609 138 84 3 11 139 20 157 3.77e-16 75.9
MS.gene012913.t1 MTR_5g014040 39.024 123 69 3 8 128 14 132 1.03e-15 74.7
MS.gene012913.t1 MTR_5g014040 60.000 45 18 0 137 181 316 360 5.39e-13 66.6
MS.gene012913.t1 MTR_5g014040 39.024 123 69 3 8 128 14 132 1.03e-15 74.7
MS.gene012913.t1 MTR_5g014040 60.000 45 18 0 137 181 316 360 5.94e-13 66.6
MS.gene012913.t1 MTR_3g084390 48.101 79 31 2 1 69 1 79 2.38e-15 68.2
MS.gene012913.t1 MTR_4g078810 60.000 45 18 0 137 181 163 207 1.44e-13 68.2
MS.gene012913.t1 MTR_4g064730 46.377 69 33 1 117 181 112 180 1.93e-13 67.4
MS.gene012913.t1 MTR_2g090675 60.000 45 18 0 137 181 166 210 3.55e-13 67.0
MS.gene012913.t1 MTR_4g098870 34.426 122 76 2 8 128 14 132 3.91e-13 67.0
MS.gene012913.t1 MTR_4g098870 60.000 45 18 0 137 181 315 359 5.63e-13 66.6
MS.gene012913.t1 MTR_5g014040 60.000 45 18 0 137 181 276 320 5.23e-13 66.6
MS.gene012913.t1 MTR_4g098870 34.426 122 76 2 8 128 14 132 5.64e-13 66.6
MS.gene012913.t1 MTR_4g098870 60.000 45 18 0 137 181 315 359 7.51e-13 66.2
MS.gene012913.t1 MTR_4g098870 34.426 122 76 2 8 128 53 171 5.67e-13 66.6
MS.gene012913.t1 MTR_4g098870 60.000 45 18 0 137 181 354 398 7.34e-13 66.2
MS.gene012913.t1 MTR_4g098870 34.426 122 76 2 8 128 34 152 5.68e-13 66.6
MS.gene012913.t1 MTR_4g098870 60.000 45 18 0 137 181 335 379 7.56e-13 66.2
MS.gene012913.t1 MTR_4g098870 34.426 122 76 2 8 128 34 152 7.09e-13 66.2
MS.gene012913.t1 MTR_4g098870 60.000 45 18 0 137 181 335 379 9.81e-13 65.9
MS.gene012913.t1 MTR_6g088950 30.682 88 61 0 47 134 43 130 1.36e-12 63.9
MS.gene012913.t1 MTR_7g089010 54.167 48 22 0 134 181 93 140 6.99e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012913.t1 AT4G31920 52.679 224 60 1 6 183 11 234 1.55e-73 231
MS.gene012913.t1 AT5G58080 48.523 237 64 3 2 181 8 243 4.02e-58 192
MS.gene012913.t1 AT2G25180 67.969 128 41 0 3 130 8 135 5.52e-58 191
MS.gene012913.t1 AT2G25180 66.000 50 17 0 132 181 195 244 8.89e-17 77.8
MS.gene012913.t1 AT1G67710 43.697 238 67 5 9 181 8 243 2.48e-52 175
MS.gene012913.t1 AT3G16857 54.167 144 64 1 8 151 33 174 1.63e-48 167
MS.gene012913.t1 AT3G16857 76.087 46 11 0 136 181 241 286 5.87e-17 78.2
MS.gene012913.t1 AT3G16857 54.167 144 64 1 8 151 33 174 1.99e-48 167
MS.gene012913.t1 AT3G16857 76.087 46 11 0 136 181 241 286 5.97e-17 78.2
MS.gene012913.t1 AT2G01760 56.349 126 54 1 5 129 4 129 2.46e-42 145
MS.gene012913.t1 AT2G01760 63.462 52 19 0 132 183 200 251 5.39e-17 77.8
MS.gene012913.t1 AT2G01760 46.104 154 54 2 5 129 4 157 2.12e-36 130
MS.gene012913.t1 AT2G01760 63.462 52 19 0 132 183 228 279 6.40e-17 77.8
MS.gene012913.t1 AT3G16857 56.190 105 44 1 47 151 2 104 1.28e-34 128
MS.gene012913.t1 AT3G16857 76.087 46 11 0 136 181 171 216 5.77e-17 78.2
MS.gene012913.t1 AT1G67710 42.222 180 37 5 67 181 1 178 2.47e-31 118
MS.gene012913.t1 AT5G49240 30.705 241 106 3 5 184 35 275 3.27e-29 109
MS.gene012913.t1 AT1G49190 38.211 123 76 0 7 129 29 151 2.52e-23 95.9
MS.gene012913.t1 AT1G49190 38.211 123 76 0 7 129 29 151 2.19e-22 94.0
MS.gene012913.t1 AT5G24470 37.063 143 79 4 6 138 42 183 4.31e-22 92.8
MS.gene012913.t1 AT2G01760 64.062 64 22 1 67 129 1 64 2.79e-21 89.0
MS.gene012913.t1 AT2G01760 63.462 52 19 0 132 183 135 186 3.62e-17 77.8
MS.gene012913.t1 AT5G60100 38.400 125 74 1 12 133 64 188 4.40e-21 90.1
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 5.09e-21 89.4
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 5.09e-21 89.4
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 5.09e-21 89.4
MS.gene012913.t1 AT5G02810 38.710 124 73 1 12 132 78 201 5.67e-21 89.7
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 1.00e-20 89.0
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 1.00e-20 89.0
MS.gene012913.t1 AT5G60100 38.843 121 71 1 12 129 64 184 1.00e-20 89.0
MS.gene012913.t1 AT2G46790 37.705 122 71 2 12 129 37 157 1.46e-20 88.2
MS.gene012913.t1 AT2G46790 37.705 122 71 2 12 129 37 157 1.62e-20 88.2
MS.gene012913.t1 AT3G62670 41.748 103 60 0 27 129 54 156 6.49e-20 86.3
MS.gene012913.t1 AT1G67710 35.625 160 36 5 87 181 12 169 3.51e-18 81.6
MS.gene012913.t1 AT5G61380 34.711 121 76 1 12 129 19 139 1.04e-17 80.5
MS.gene012913.t1 AT5G44190 68.889 45 14 0 137 181 152 196 4.84e-15 72.4
MS.gene012913.t1 AT5G44190 68.889 45 14 0 137 181 152 196 5.09e-15 72.4
MS.gene012913.t1 AT2G20570 68.889 45 14 0 137 181 158 202 3.40e-14 70.1
MS.gene012913.t1 AT2G20570 68.889 45 14 0 137 181 174 218 4.03e-14 69.7
MS.gene012913.t1 AT2G40970 56.250 48 21 0 134 181 107 154 3.86e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 50.909 55 27 0 129 183 293 347 8.63e-13 66.2
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 50.909 55 27 0 129 183 293 347 8.63e-13 66.2
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 50.909 55 27 0 129 183 293 347 8.63e-13 66.2
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 50.909 55 27 0 129 183 293 347 8.63e-13 66.2
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 50.909 55 27 0 129 183 293 347 8.63e-13 66.2
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT4G18020 35.385 130 75 3 6 128 12 139 6.12e-13 66.6
MS.gene012913.t1 AT4G18020 59.574 47 19 0 137 183 301 347 9.69e-13 65.9
MS.gene012913.t1 AT5G59570 56.250 48 21 0 134 181 143 190 1.10e-12 65.1
MS.gene012913.t1 AT5G59570 56.250 48 21 0 134 181 143 190 1.10e-12 65.1
MS.gene012913.t1 AT3G46640 56.250 48 21 0 134 181 146 193 1.60e-12 65.1
MS.gene012913.t1 AT3G46640 56.250 48 21 0 134 181 146 193 1.60e-12 65.1
MS.gene012913.t1 AT3G46640 56.250 48 21 0 134 181 146 193 1.69e-12 64.7
MS.gene012913.t1 AT5G07210 31.667 120 79 2 12 130 16 133 3.57e-12 64.3
MS.gene012913.t1 AT5G05090 54.167 48 22 0 134 181 83 130 4.38e-12 63.2
MS.gene012913.t1 AT3G10760 56.522 46 20 0 136 181 109 154 4.67e-12 63.5

Find 51 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCCTTCTCTCTTGCTTT+TGG 0.208333 3.4:-90260178 MS.gene012913:CDS
GTCCCAGTTTGTTTGGTCTA+TGG 0.314039 3.4:-90259472 MS.gene012913:CDS
CTTGAAGCTGTTAATCAGTT+AGG 0.332985 3.4:-90259432 MS.gene012913:CDS
GGAACTTGTGGGACTTGAAA+TGG 0.353940 3.4:-90259765 MS.gene012913:CDS
TCTGCTGAAACCTGTGAGAT+TGG 0.389884 3.4:-90259538 MS.gene012913:CDS
TGGAGGAGCTAAGGAACATT+TGG 0.390210 3.4:-90259518 MS.gene012913:CDS
GTTCCATAGACCAAACAAAC+TGG 0.396655 3.4:+90259469 None:intergenic
ATACGGAGTCCCAGTTTGTT+TGG 0.408262 3.4:-90259479 MS.gene012913:CDS
ATTTCAGTGTTGTCTGCATA+TGG 0.417968 3.4:-90259609 MS.gene012913:intron
ACATTTGGCAGCATGTAATT+CGG 0.438242 3.4:-90259503 MS.gene012913:CDS
GCAGCATGTAATTCGGAATA+CGG 0.461939 3.4:-90259496 MS.gene012913:CDS
AGCTTAAGTCCATCCATGTC+AGG 0.462326 3.4:+90259791 None:intergenic
GGACGTGGCGACCCATCTCC+AGG 0.480850 3.4:-90259263 MS.gene012913:CDS
CACAACTAGTAATGTAATAA+CGG 0.482381 3.4:-90259876 MS.gene012913:CDS
ACTAGAAAAGTGCCTGGAGA+TGG 0.493120 3.4:+90259251 None:intergenic
CGTTATTACATTACTAGTTG+TGG 0.495202 3.4:+90259877 None:intergenic
CTTAGCTCCTCCAATCTCAC+AGG 0.496928 3.4:+90259528 None:intergenic
CAGAAAATCACGGGCACCGT+GGG 0.504818 3.4:+90259557 None:intergenic
TAGAGGACTAGAAAAGTGCC+TGG 0.518904 3.4:+90259245 None:intergenic
TGTGAACTTCAGCGATTACC+AGG 0.522457 3.4:+90259816 None:intergenic
CTAGAAAAGTGCCTGGAGAT+GGG 0.532146 3.4:+90259252 None:intergenic
TCCAAAAGCAAGAGAGAAGG+AGG 0.535506 3.4:+90260177 None:intergenic
CTTAAGCTGCTGGAACTTGT+GGG 0.537675 3.4:-90259776 MS.gene012913:CDS
CTGTGAGATTGGAGGAGCTA+AGG 0.543793 3.4:-90259527 MS.gene012913:CDS
TCCACTGATCGGTTTCCCAT+CGG 0.547632 3.4:-90260228 MS.gene012913:CDS
AGCGAGAACACGCATTCCGA+TGG 0.550357 3.4:+90260212 None:intergenic
ACTTAAGCTGCTGGAACTTG+TGG 0.552160 3.4:-90259777 MS.gene012913:CDS
GCTGAAACCTGTGAGATTGG+AGG 0.556603 3.4:-90259535 MS.gene012913:CDS
TGCATATGGTGATACGGAGC+TGG 0.563561 3.4:-90259595 MS.gene012913:CDS
CATGGATGGACTTAAGCTGC+TGG 0.564074 3.4:-90259786 MS.gene012913:CDS
GACCGTGGTATTGACATGAT+CGG 0.585397 3.4:+90260145 None:intergenic
CAGGTTTCAGCAGAAAATCA+CGG 0.587467 3.4:+90259547 None:intergenic
GTTTCCAAAAGCAAGAGAGA+AGG 0.592241 3.4:+90260174 None:intergenic
TAATGGATGATTCCACTGAT+CGG 0.598611 3.4:-90260239 MS.gene012913:CDS
GTTGTCTGCATATGGTGATA+CGG 0.598779 3.4:-90259601 MS.gene012913:CDS
TGATACGGAGCTGGTGATGA+AGG 0.599556 3.4:-90259586 MS.gene012913:CDS
TCCGATGGGAAACCGATCAG+TGG 0.602032 3.4:+90260227 None:intergenic
AAATCTTACAAGAGAGGACG+TGG 0.604587 3.4:-90259278 MS.gene012913:CDS
AGGTTTCAGCAGAAAATCAC+GGG 0.606322 3.4:+90259548 None:intergenic
CCCACTTACTTATGACAGGA+AGG 0.611035 3.4:+90259741 None:intergenic
TTCCATAGACCAAACAAACT+GGG 0.611190 3.4:+90259470 None:intergenic
GCGAGAACACGCATTCCGAT+GGG 0.611220 3.4:+90260213 None:intergenic
GTTCACATGCCTGACATGGA+TGG 0.616361 3.4:-90259800 MS.gene012913:CDS
TAACAGCTTCAAGAAACTTG+CGG 0.617068 3.4:+90259442 None:intergenic
TGTTGAAAATCTTACAAGAG+AGG 0.626999 3.4:-90259284 MS.gene012913:CDS
TGAAGTTCACATGCCTGACA+TGG 0.628648 3.4:-90259804 MS.gene012913:CDS
GTGATGAAGGCAATTTCCCA+CGG 0.657007 3.4:-90259573 MS.gene012913:CDS
AAACATAGACATGTTTGACC+TGG 0.663608 3.4:-90259834 MS.gene012913:CDS
CGTGGTATTGACATGATCGG+AGG 0.677686 3.4:+90260148 None:intergenic
GCAGAAAATCACGGGCACCG+TGG 0.679138 3.4:+90259556 None:intergenic
CTCCGATCATGTCAATACCA+CGG 0.680497 3.4:-90260147 MS.gene012913:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAATATTATACACATAAAT+TGG + chr3.4:90259394-90259413 None:intergenic 15.0%
!! GTACAAAGTATAATAAAAAT+TGG + chr3.4:90259441-90259460 None:intergenic 15.0%
!!! ATATCTCTCTTATTTATTTA+TGG - chr3.4:90259573-90259592 MS.gene012913:CDS 15.0%
!!! ATCAATTCAAAAATCTTTTT+GGG + chr3.4:90260182-90260201 None:intergenic 15.0%
!!! CATCAATTCAAAAATCTTTT+TGG + chr3.4:90260183-90260202 None:intergenic 20.0%
!!! TCTCTTATTTATTTATGGTT+GGG - chr3.4:90259578-90259597 MS.gene012913:CDS 20.0%
! CACAACTAGTAATGTAATAA+CGG - chr3.4:90259613-90259632 MS.gene012913:intron 25.0%
!! CTCTCTTATTTATTTATGGT+TGG - chr3.4:90259577-90259596 MS.gene012913:CDS 25.0%
!! CTCTTATTTATTTATGGTTG+GGG - chr3.4:90259579-90259598 MS.gene012913:CDS 25.0%
!!! TCATGTTAATGTTGTTTTAC+TGG - chr3.4:90260145-90260164 MS.gene012913:intron 25.0%
AAAGTACACTTTGAATGACA+AGG + chr3.4:90259539-90259558 None:intergenic 30.0%
CGTTATTACATTACTAGTTG+TGG + chr3.4:90259615-90259634 None:intergenic 30.0%
TGTTGAAAATCTTACAAGAG+AGG - chr3.4:90260205-90260224 MS.gene012913:CDS 30.0%
!! TCAGAATCTCTATTTCTACA+TGG - chr3.4:90259478-90259497 MS.gene012913:CDS 30.0%
AAACATAGACATGTTTGACC+TGG - chr3.4:90259655-90259674 MS.gene012913:intron 35.0%
ACATTTGGCAGCATGTAATT+CGG - chr3.4:90259986-90260005 MS.gene012913:intron 35.0%
ACTTTGTACCTGTTATTGCT+TGG - chr3.4:90259452-90259471 MS.gene012913:CDS 35.0%
ATTTCAGTGTTGTCTGCATA+TGG - chr3.4:90259880-90259899 MS.gene012913:CDS 35.0%
CTTGAAGCTGTTAATCAGTT+AGG - chr3.4:90260057-90260076 MS.gene012913:intron 35.0%
TAACAGCTTCAAGAAACTTG+CGG + chr3.4:90260050-90260069 None:intergenic 35.0%
TTCCATAGACCAAACAAACT+GGG + chr3.4:90260022-90260041 None:intergenic 35.0%
! TCTGAAATCCAAGCAATAAC+AGG + chr3.4:90259463-90259482 None:intergenic 35.0%
!!! TAATGTTGTTTTACTGGCAG+AGG - chr3.4:90260151-90260170 MS.gene012913:CDS 35.0%
AAATCTTACAAGAGAGGACG+TGG - chr3.4:90260211-90260230 MS.gene012913:CDS 40.0%
ACCTTCCTGTCATAAGTAAG+TGG - chr3.4:90259747-90259766 MS.gene012913:intron 40.0%
AGGTTTCAGCAGAAAATCAC+GGG + chr3.4:90259944-90259963 None:intergenic 40.0%
CAGGTTTCAGCAGAAAATCA+CGG + chr3.4:90259945-90259964 None:intergenic 40.0%
CCTTCCTGTCATAAGTAAGT+GGG - chr3.4:90259748-90259767 MS.gene012913:intron 40.0%
CTTCCTGTCATAAGTAAGTG+GGG - chr3.4:90259749-90259768 MS.gene012913:intron 40.0%
GCAGCATGTAATTCGGAATA+CGG - chr3.4:90259993-90260012 MS.gene012913:intron 40.0%
GTTCCATAGACCAAACAAAC+TGG + chr3.4:90260023-90260042 None:intergenic 40.0%
GTTGTCTGCATATGGTGATA+CGG - chr3.4:90259888-90259907 MS.gene012913:intron 40.0%
GTTTCCAAAAGCAAGAGAGA+AGG + chr3.4:90259318-90259337 None:intergenic 40.0%
TATCAAAAAGAAGCAGACCG+TGG + chr3.4:90259362-90259381 None:intergenic 40.0%
TTCCTGTCATAAGTAAGTGG+GGG - chr3.4:90259750-90259769 MS.gene012913:CDS 40.0%
ACTTAAGCTGCTGGAACTTG+TGG - chr3.4:90259712-90259731 MS.gene012913:intron 45.0%
AGCTTAAGTCCATCCATGTC+AGG + chr3.4:90259701-90259720 None:intergenic 45.0%
ATCCCCCACTTACTTATGAC+AGG + chr3.4:90259755-90259774 None:intergenic 45.0%
CCCACTTACTTATGACAGGA+AGG + chr3.4:90259751-90259770 None:intergenic 45.0%
CTCCGATCATGTCAATACCA+CGG - chr3.4:90259342-90259361 MS.gene012913:intron 45.0%
CTTAAGCTGCTGGAACTTGT+GGG - chr3.4:90259713-90259732 MS.gene012913:intron 45.0%
GGAACTTGTGGGACTTGAAA+TGG - chr3.4:90259724-90259743 MS.gene012913:intron 45.0%
GTGATGAAGGCAATTTCCCA+CGG - chr3.4:90259916-90259935 MS.gene012913:intron 45.0%
TCCAAAAGCAAGAGAGAAGG+AGG + chr3.4:90259315-90259334 None:intergenic 45.0%
TCTGCTGAAACCTGTGAGAT+TGG - chr3.4:90259951-90259970 MS.gene012913:intron 45.0%
TGAAGTTCACATGCCTGACA+TGG - chr3.4:90259685-90259704 MS.gene012913:intron 45.0%
TGGAGGAGCTAAGGAACATT+TGG - chr3.4:90259971-90259990 MS.gene012913:intron 45.0%
TGTGAACTTCAGCGATTACC+AGG + chr3.4:90259676-90259695 None:intergenic 45.0%
! ATACGGAGTCCCAGTTTGTT+TGG - chr3.4:90260010-90260029 MS.gene012913:intron 45.0%
! GTCCCAGTTTGTTTGGTCTA+TGG - chr3.4:90260017-90260036 MS.gene012913:intron 45.0%
! TCCTCCTTCTCTCTTGCTTT+TGG - chr3.4:90259311-90259330 MS.gene012913:CDS 45.0%
!! CTAGAAAAGTGCCTGGAGAT+GGG + chr3.4:90260240-90260259 None:intergenic 45.0%
!! GACCGTGGTATTGACATGAT+CGG + chr3.4:90259347-90259366 None:intergenic 45.0%
CTGTGAGATTGGAGGAGCTA+AGG - chr3.4:90259962-90259981 MS.gene012913:intron 50.0%
CTTAGCTCCTCCAATCTCAC+AGG + chr3.4:90259964-90259983 None:intergenic 50.0%
GCTGAAACCTGTGAGATTGG+AGG - chr3.4:90259954-90259973 MS.gene012913:intron 50.0%
GTTCACATGCCTGACATGGA+TGG - chr3.4:90259689-90259708 MS.gene012913:intron 50.0%
TGCATATGGTGATACGGAGC+TGG - chr3.4:90259894-90259913 MS.gene012913:intron 50.0%
! CATGGATGGACTTAAGCTGC+TGG - chr3.4:90259703-90259722 MS.gene012913:intron 50.0%
! TCCACTGATCGGTTTCCCAT+CGG - chr3.4:90259261-90259280 MS.gene012913:CDS 50.0%
! TGATACGGAGCTGGTGATGA+AGG - chr3.4:90259903-90259922 MS.gene012913:intron 50.0%
!! CGTGGTATTGACATGATCGG+AGG + chr3.4:90259344-90259363 None:intergenic 50.0%
AGCGAGAACACGCATTCCGA+TGG + chr3.4:90259280-90259299 None:intergenic 55.0%
GCGAGAACACGCATTCCGAT+GGG + chr3.4:90259279-90259298 None:intergenic 55.0%
TCCGATGGGAAACCGATCAG+TGG + chr3.4:90259265-90259284 None:intergenic 55.0%
! CAGAAAATCACGGGCACCGT+GGG + chr3.4:90259935-90259954 None:intergenic 55.0%
! GCAGAAAATCACGGGCACCG+TGG + chr3.4:90259936-90259955 None:intergenic 60.0%
GGACGTGGCGACCCATCTCC+AGG - chr3.4:90260226-90260245 MS.gene012913:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 90259252 90260259 90259252 ID=MS.gene012913
chr3.4 mRNA 90259252 90260259 90259252 ID=MS.gene012913.t1;Parent=MS.gene012913
chr3.4 exon 90260148 90260259 90260148 ID=MS.gene012913.t1.exon1;Parent=MS.gene012913.t1
chr3.4 CDS 90260148 90260259 90260148 ID=cds.MS.gene012913.t1;Parent=MS.gene012913.t1
chr3.4 exon 90259750 90259902 90259750 ID=MS.gene012913.t1.exon2;Parent=MS.gene012913.t1
chr3.4 CDS 90259750 90259902 90259750 ID=cds.MS.gene012913.t1;Parent=MS.gene012913.t1
chr3.4 exon 90259424 90259624 90259424 ID=MS.gene012913.t1.exon3;Parent=MS.gene012913.t1
chr3.4 CDS 90259424 90259624 90259424 ID=cds.MS.gene012913.t1;Parent=MS.gene012913.t1
chr3.4 exon 90259252 90259340 90259252 ID=MS.gene012913.t1.exon4;Parent=MS.gene012913.t1
chr3.4 CDS 90259252 90259340 90259252 ID=cds.MS.gene012913.t1;Parent=MS.gene012913.t1
Gene Sequence

>MS.gene012913

ATGGATGATTCCACTGATCGGTTTCCCATCGGAATGCGTGTTCTCGCTGTCGACGACAATCCTCCTTCTCTCTTGCTTTTGGAAACTCTCCTCCGATCATGTCAATACCACGGTCTGCTTCTTTTTGATACTTTTGTGTCCAATTTATGTGTATAATATTACTAATAATGATGATATACTTTATGTCCAATTTTTATTATACTTTGTACCTGTTATTGCTTGGATTTCAGAATCTCTATTTCTACATGGTTAGACTCTTTTGTTTTTCAAGTATTTCACAATTGCCTTGTCATTCAAAGTGTACTTTGTTCGTTCTGAATAATATCTCTCTTATTTATTTATGGTTGGGGATTGCAGTAACCACAACTAGTAATGTAATAACGGCATTGACTATGTTGCGAGAAAACATAGACATGTTTGACCTGGTAATCGCTGAAGTTCACATGCCTGACATGGATGGACTTAAGCTGCTGGAACTTGTGGGACTTGAAATGGACCTTCCTGTCATAAGTAAGTGGGGGATTGTTCTGTAATATTCAGTTCTTCATCTTTGTTCTGTTTCTTCATTTTCACTCTCATTTATTCATATTATTATTATTAATCAATGATTGTTGTTGTAAATTGTTTGATTTCAGTGTTGTCTGCATATGGTGATACGGAGCTGGTGATGAAGGCAATTTCCCACGGTGCCCGTGATTTTCTGCTGAAACCTGTGAGATTGGAGGAGCTAAGGAACATTTGGCAGCATGTAATTCGGAATACGGAGTCCCAGTTTGTTTGGTCTATGGAACTGCACCGCAAGTTTCTTGAAGCTGTTAATCAGTTAGGAGTTGACAGTATGTTCTTCACGCTGTCTGCAACACTAAAACAATTTCATTTCTCGAGTTTTAGTCTCATGTTAATGTTGTTTTACTGGCAGAGGCTGTACCCAAAAAGATTTTTGAATTGATGAATGTTGAAAATCTTACAAGAGAGGACGTGGCGACCCATCTCCAGGCACTTTTCTAG
Protein sequence

>MS.gene012913.t1

MDDSTDRFPIGMRVLAVDDNPPSLLLLETLLRSCQYHVTTTSNVITALTMLRENIDMFDLVIAEVHMPDMDGLKLLELVGLEMDLPVIMLSAYGDTELVMKAISHGARDFLLKPVRLEELRNIWQHVIRNTESQFVWSMELHRKFLEAVNQLGVDKAVPKKIFELMNVENLTREDVATHLQALF