AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene012916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012916.t1 MTR_3g102590 94.872 195 9 1 1 194 1 195 1.69e-123 347
MS.gene012916.t1 MTR_3g102590 94.792 192 9 1 1 191 1 192 6.95e-121 342
MS.gene012916.t1 MTR_3g102600 71.649 194 45 1 1 194 1 184 1.99e-92 268
MS.gene012916.t1 MTR_3g102600 71.728 191 44 1 1 191 1 181 8.05e-90 263
MS.gene012916.t1 MTR_1g013170 52.720 239 61 2 5 191 2 240 7.69e-76 236
MS.gene012916.t1 MTR_7g117705 49.791 239 68 3 5 191 2 240 1.51e-70 221
MS.gene012916.t1 MTR_1g013180 49.791 239 68 2 5 191 153 391 6.47e-68 219
MS.gene012916.t1 MTR_4g121020 49.372 239 71 2 3 191 26 264 8.28e-68 219
MS.gene012916.t1 MTR_4g121020 49.372 239 71 2 3 191 26 264 8.87e-68 219
MS.gene012916.t1 MTR_3g102600 68.276 145 36 1 50 194 1 135 6.28e-66 199
MS.gene012916.t1 MTR_2g034960 47.234 235 76 2 5 191 26 260 2.39e-65 211
MS.gene012916.t1 MTR_4g131600 48.485 231 72 1 8 191 20 250 1.63e-64 209
MS.gene012916.t1 MTR_3g102600 68.310 142 35 1 50 191 1 132 1.03e-63 195
MS.gene012916.t1 MTR_3g106220 73.881 134 35 0 1 134 1 134 5.62e-63 206
MS.gene012916.t1 MTR_3g106220 83.051 59 10 0 133 191 189 247 2.05e-25 102
MS.gene012916.t1 MTR_1g032570 46.091 243 76 2 4 191 7 249 2.03e-62 204
MS.gene012916.t1 MTR_4g131580 45.188 239 82 1 2 191 11 249 6.01e-61 199
MS.gene012916.t1 MTR_4g131570 45.763 236 76 1 8 191 17 252 1.08e-59 196
MS.gene012916.t1 MTR_3g086100 43.644 236 81 2 8 191 13 248 4.01e-58 191
MS.gene012916.t1 MTR_8g079940 44.492 236 81 2 6 191 23 258 4.70e-56 185
MS.gene012916.t1 MTR_2g450070 62.595 131 49 0 2 132 12 142 3.90e-50 172
MS.gene012916.t1 MTR_2g450070 70.370 54 16 0 138 191 209 262 8.61e-18 80.9
MS.gene012916.t1 MTR_2g450070 62.595 131 49 0 2 132 12 142 4.05e-50 172
MS.gene012916.t1 MTR_2g450070 70.370 54 16 0 138 191 209 262 9.68e-18 80.9
MS.gene012916.t1 MTR_4g021760 41.121 214 100 3 3 191 6 218 2.25e-49 162
MS.gene012916.t1 MTR_4g021845 36.792 212 108 3 8 194 10 220 3.12e-43 144
MS.gene012916.t1 MTR_4g021790 36.842 209 106 3 8 191 10 217 6.53e-40 137
MS.gene012916.t1 MTR_4g021855 35.885 209 108 3 8 191 10 217 2.19e-39 136
MS.gene012916.t1 MTR_1g032570 41.667 180 50 2 67 191 1 180 1.47e-38 139
MS.gene012916.t1 MTR_0450s0040 32.701 211 111 1 12 191 8 218 2.83e-34 124
MS.gene012916.t1 MTR_3g086100 40.113 177 54 2 67 191 1 177 3.08e-34 126
MS.gene012916.t1 MTR_7g026400 35.751 193 88 1 35 191 14 206 6.79e-33 124
MS.gene012916.t1 MTR_6g045327 33.962 212 114 3 7 193 13 223 5.36e-32 119
MS.gene012916.t1 MTR_1g013160 42.568 148 33 2 70 165 1 148 2.02e-30 109
MS.gene012916.t1 MTR_4g121020 41.935 155 40 2 87 191 6 160 6.43e-29 112
MS.gene012916.t1 MTR_5g055260 35.567 194 102 6 8 191 10 190 3.55e-28 106
MS.gene012916.t1 MTR_8g105600 31.304 230 110 5 8 191 9 236 6.17e-27 107
MS.gene012916.t1 MTR_3g084390 56.962 79 24 2 1 69 1 79 7.44e-24 90.1
MS.gene012916.t1 MTR_8g093040 36.486 148 91 2 1 145 7 154 7.79e-22 90.1
MS.gene012916.t1 MTR_6g016850 34.375 128 81 2 5 130 6 132 2.73e-21 86.3
MS.gene012916.t1 MTR_4g023980 32.374 139 69 2 55 168 36 174 1.21e-20 84.7
MS.gene012916.t1 MTR_5g014040 38.400 125 71 3 8 130 14 134 4.75e-19 84.3
MS.gene012916.t1 MTR_5g014040 55.000 60 26 1 135 194 304 362 8.11e-13 66.6
MS.gene012916.t1 MTR_5g014040 38.400 125 71 3 8 130 14 134 4.96e-19 84.3
MS.gene012916.t1 MTR_5g014040 54.386 57 26 0 135 191 304 360 8.48e-13 66.2
MS.gene012916.t1 MTR_3g092780 30.357 168 108 3 12 171 56 222 6.13e-19 84.3
MS.gene012916.t1 MTR_3g092780 30.357 168 108 3 12 171 56 222 6.23e-19 84.3
MS.gene012916.t1 MTR_3g092780 30.539 167 108 3 12 171 56 221 1.71e-18 82.8
MS.gene012916.t1 MTR_3g092780 30.539 167 108 3 12 171 56 221 1.74e-18 82.8
MS.gene012916.t1 MTR_8g024260 35.833 120 74 1 14 130 38 157 3.54e-18 82.0
MS.gene012916.t1 MTR_7g118260 31.788 151 97 3 1 145 1 151 3.61e-18 82.0
MS.gene012916.t1 MTR_7g118260 29.240 171 116 2 1 166 1 171 2.16e-17 79.7
MS.gene012916.t1 MTR_1g067110 30.986 142 95 1 12 150 85 226 4.06e-17 79.0
MS.gene012916.t1 MTR_1g067110 30.986 142 95 1 12 150 85 226 4.32e-17 79.0
MS.gene012916.t1 MTR_4g098870 32.520 123 81 1 8 130 34 154 9.12e-17 77.8
MS.gene012916.t1 MTR_4g098870 53.030 66 28 2 127 191 316 379 1.04e-12 66.2
MS.gene012916.t1 MTR_4g098870 32.520 123 81 1 8 130 53 173 1.01e-16 77.8
MS.gene012916.t1 MTR_4g098870 53.030 66 28 2 127 191 335 398 1.26e-12 65.9
MS.gene012916.t1 MTR_4g098870 32.520 123 81 1 8 130 14 134 1.01e-16 77.8
MS.gene012916.t1 MTR_4g098870 53.030 66 28 2 127 191 296 359 1.05e-12 66.2
MS.gene012916.t1 MTR_4g098870 32.520 123 81 1 8 130 34 154 1.05e-16 77.8
MS.gene012916.t1 MTR_4g098870 53.030 66 28 2 127 191 316 379 1.31e-12 65.9
MS.gene012916.t1 MTR_4g098870 32.520 123 81 1 8 130 14 134 1.07e-16 77.8
MS.gene012916.t1 MTR_4g098870 53.030 66 28 2 127 191 296 359 1.26e-12 65.9
MS.gene012916.t1 MTR_3g037390 31.452 124 82 1 12 132 24 147 1.50e-16 77.4
MS.gene012916.t1 MTR_4g061360 34.426 122 77 2 12 130 89 210 3.06e-16 76.6
MS.gene012916.t1 MTR_4g108880 30.645 124 83 1 12 132 33 156 3.84e-16 76.3
MS.gene012916.t1 MTR_6g088950 32.292 96 65 0 38 133 34 129 2.79e-13 65.9
MS.gene012916.t1 MTR_5g014040 55.000 60 26 1 135 194 264 322 8.23e-13 66.2
MS.gene012916.t1 MTR_8g032710 31.933 119 80 1 11 128 20 138 2.21e-12 65.1
MS.gene012916.t1 MTR_4g078810 49.057 53 27 0 139 191 155 207 9.73e-12 63.2
MS.gene012916.t1 MTR_2g090675 49.057 53 27 0 139 191 158 210 2.06e-11 62.4
MS.gene012916.t1 MTR_4g064730 54.902 51 23 0 141 191 130 180 2.15e-11 62.0
MS.gene012916.t1 MTR_7g089010 52.941 51 24 0 141 191 90 140 6.29e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene012916.t1 AT2G25180 52.321 237 65 2 3 191 8 244 1.45e-75 238
MS.gene012916.t1 AT4G31920 53.153 222 68 1 6 191 11 232 1.69e-75 236
MS.gene012916.t1 AT5G58080 45.570 237 81 2 2 191 8 243 4.06e-60 198
MS.gene012916.t1 AT1G67710 46.186 236 74 3 9 191 8 243 1.29e-58 192
MS.gene012916.t1 AT2G01760 40.650 246 87 3 5 191 4 249 1.49e-52 172
MS.gene012916.t1 AT2G01760 36.496 274 87 4 5 191 4 277 2.02e-46 157
MS.gene012916.t1 AT3G16857 53.103 145 56 1 8 152 33 165 3.53e-44 155
MS.gene012916.t1 AT3G16857 57.692 78 23 1 124 191 209 286 2.84e-20 88.2
MS.gene012916.t1 AT3G16857 53.103 145 56 1 8 152 33 165 4.31e-44 155
MS.gene012916.t1 AT3G16857 57.692 78 23 1 124 191 209 286 2.68e-20 88.2
MS.gene012916.t1 AT5G49240 32.365 241 112 2 5 194 35 275 2.12e-37 131
MS.gene012916.t1 AT1G67710 43.820 178 47 3 67 191 1 178 5.31e-35 128
MS.gene012916.t1 AT3G62670 34.599 237 106 5 2 191 31 265 1.45e-33 124
MS.gene012916.t1 AT3G16857 53.774 106 37 1 47 152 2 95 2.05e-31 120
MS.gene012916.t1 AT3G16857 57.692 78 23 1 124 191 139 216 2.86e-20 88.2
MS.gene012916.t1 AT1G49190 31.667 240 109 5 7 191 29 268 4.01e-31 117
MS.gene012916.t1 AT1G49190 39.200 125 76 0 7 131 29 153 4.56e-25 102
MS.gene012916.t1 AT1G67710 39.873 158 42 3 87 191 12 169 6.91e-24 97.8
MS.gene012916.t1 AT2G01760 33.152 184 64 5 67 191 1 184 1.92e-21 89.7
MS.gene012916.t1 AT2G46790 34.074 135 86 1 12 143 37 171 1.23e-19 85.9
MS.gene012916.t1 AT2G46790 34.074 135 86 1 12 143 37 171 1.32e-19 85.9
MS.gene012916.t1 AT5G24470 33.600 125 80 1 10 131 48 172 3.91e-19 84.7
MS.gene012916.t1 AT5G61380 33.065 124 80 1 12 132 19 142 1.09e-18 83.6
MS.gene012916.t1 AT5G02810 35.246 122 76 1 12 130 78 199 1.94e-16 77.0
MS.gene012916.t1 AT5G60100 33.858 127 80 2 8 130 59 185 2.42e-16 76.6
MS.gene012916.t1 AT5G60100 33.858 127 80 2 8 130 59 185 2.42e-16 76.6
MS.gene012916.t1 AT5G60100 33.858 127 80 2 8 130 59 185 2.42e-16 76.6
MS.gene012916.t1 AT5G60100 34.426 122 77 1 12 130 64 185 2.75e-16 76.6
MS.gene012916.t1 AT5G60100 34.426 122 77 1 12 130 64 185 5.77e-16 75.5
MS.gene012916.t1 AT5G60100 34.426 122 77 1 12 130 64 185 5.77e-16 75.5
MS.gene012916.t1 AT5G60100 34.426 122 77 1 12 130 64 185 5.77e-16 75.5
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.49e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.21e-11 63.2
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.79e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.41e-11 62.8
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.79e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.41e-11 62.8
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.79e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.41e-11 62.8
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.79e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.41e-11 62.8
MS.gene012916.t1 AT4G18020 34.091 132 78 3 6 130 12 141 4.79e-15 73.2
MS.gene012916.t1 AT4G18020 48.485 66 32 1 128 191 280 345 1.41e-11 62.8
MS.gene012916.t1 AT5G44190 59.615 52 21 0 140 191 145 196 1.17e-13 68.6
MS.gene012916.t1 AT5G44190 59.615 52 21 0 140 191 145 196 1.43e-13 68.6
MS.gene012916.t1 AT5G07210 29.012 162 95 4 12 172 16 158 3.01e-13 67.8
MS.gene012916.t1 AT3G46640 54.237 59 25 1 135 191 135 193 2.38e-12 64.7
MS.gene012916.t1 AT3G46640 54.237 59 25 1 135 191 135 193 2.49e-12 64.7
MS.gene012916.t1 AT3G46640 54.237 59 25 1 135 191 135 193 2.49e-12 64.7
MS.gene012916.t1 AT5G59570 52.542 59 26 1 135 191 132 190 6.19e-12 63.5
MS.gene012916.t1 AT5G59570 52.542 59 26 1 135 191 132 190 6.19e-12 63.5
MS.gene012916.t1 AT2G40970 54.902 51 23 0 141 191 104 154 6.81e-12 62.8
MS.gene012916.t1 AT2G20570 54.717 53 24 0 139 191 150 202 9.23e-12 63.2
MS.gene012916.t1 AT2G20570 62.222 45 17 0 147 191 174 218 1.59e-11 62.8
MS.gene012916.t1 AT5G05090 52.941 51 24 0 141 191 80 130 3.81e-11 60.8
MS.gene012916.t1 AT3G10760 52.941 51 24 0 141 191 104 154 5.64e-11 60.8

Find 50 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGGTGCCTCTGAATTTC+TGG 0.024455 3.4:-90179385 MS.gene012916:CDS
GCTGTACCCGAAAAGATTCT+TGG 0.366985 3.4:-90178937 MS.gene012916:CDS
TAGAGCAGCTAAAGACCATT+TGG 0.387046 3.4:-90179347 MS.gene012916:CDS
AGGACGTGGCAAGCCATCTC+CGG 0.418300 3.4:-90178885 MS.gene012916:CDS
CCATTTGGCAGCATGTAATT+CGG 0.418909 3.4:-90179332 MS.gene012916:CDS
AGAAGAAGTCCCAGTCTGTT+TGG 0.426238 3.4:-90179278 MS.gene012916:CDS
CACGCATTCCGATGGGAAAC+CGG 0.429749 3.4:+90180370 None:intergenic
GTTGCTGCTGTTAATCAGTT+AGG 0.449852 3.4:-90179231 MS.gene012916:CDS
CCGAATTACATGCTGCCAAA+TGG 0.458584 3.4:+90179332 None:intergenic
AGCTTAAGTCCATCCATGTC+AGG 0.462326 3.4:+90179611 None:intergenic
TCCGATGGGAAACCGGTCAC+TGG 0.476693 3.4:+90180377 None:intergenic
TTTAGCTGCTCTAATCTCAC+AGG 0.481770 3.4:+90179357 None:intergenic
GGACGTGGCAAGCCATCTCC+GGG 0.509409 3.4:-90178884 MS.gene012916:CDS
ACTAGAAAATTGCCCGGAGA+TGG 0.513115 3.4:+90178872 None:intergenic
GTCCCAGTCTGTTTGGTCTG+TGG 0.513687 3.4:-90179271 MS.gene012916:CDS
CTATAATGAGCTAGTGATGA+AGG 0.515535 3.4:-90179415 MS.gene012916:CDS
ATGCGTGTTCTCGCCGTCGA+CGG 0.517593 3.4:-90180354 MS.gene012916:CDS
GCTCCACAGACCAAACAGAC+TGG 0.522152 3.4:+90179268 None:intergenic
TTCTAAATCCGAGAGATAAG+TGG 0.525410 3.4:+90180326 None:intergenic
GGGACTTCTTCTGAGCTGAT+AGG 0.528115 3.4:+90179289 None:intergenic
GGTGCACATCAGCGATTACC+AGG 0.528972 3.4:+90179636 None:intergenic
CGGCGATTCCACTTATCTCT+CGG 0.532887 3.4:-90180334 MS.gene012916:CDS
ACTTAAGCTGCTTGAACTTG+TGG 0.534364 3.4:-90179597 MS.gene012916:CDS
GGCGAGAACACGCATTCCGA+TGG 0.535415 3.4:+90180362 None:intergenic
CTCCACAGACCAAACAGACT+GGG 0.537731 3.4:+90179269 None:intergenic
GTGCACCTGCCTGACATGGA+TGG 0.546404 3.4:-90179620 MS.gene012916:CDS
AAATATTACAAGGGAGGACG+TGG 0.547028 3.4:-90178899 MS.gene012916:CDS
TGAACTTACTTACGACCGGC+AGG 0.552218 3.4:+90179561 None:intergenic
TTATGGATGACTCCAGTGAC+CGG 0.561206 3.4:-90180389 MS.gene012916:CDS
GCATGTAATTCGGAAGAAGA+AGG 0.566666 3.4:-90179322 MS.gene012916:CDS
TGAACTTGTGGAACTTGAGA+CGG 0.569359 3.4:-90179585 MS.gene012916:CDS
GAACTGAACTTACTTACGAC+CGG 0.571061 3.4:+90179557 None:intergenic
TGATGTGCACCTGCCTGACA+TGG 0.573968 3.4:-90179624 MS.gene012916:CDS
TAAGTCCATCCATGTCAGGC+AGG 0.574028 3.4:+90179615 None:intergenic
TCCAGTGACCGGTTTCCCAT+CGG 0.576158 3.4:-90180378 MS.gene012916:CDS
TGAATGTTGAAAATATTACA+AGG 0.578744 3.4:-90178909 MS.gene012916:CDS
GACCGTGGTATTGACATGAT+CGG 0.585397 3.4:+90180295 None:intergenic
CCAGAAATTCAGAGGCACCA+TGG 0.591294 3.4:+90179385 None:intergenic
ATAAGTGGAATCGCCGTCGA+CGG 0.593520 3.4:+90180341 None:intergenic
GGAACTTGAGACGGACCTGC+CGG 0.597204 3.4:-90179576 MS.gene012916:CDS
AGGTTTCACCAGAAATTCAG+AGG 0.602943 3.4:+90179377 None:intergenic
TAAAGGACTAGAAAATTGCC+CGG 0.605859 3.4:+90178866 None:intergenic
GCGAGAACACGCATTCCGAT+GGG 0.617268 3.4:+90180363 None:intergenic
GAATGTTGAAAATATTACAA+GGG 0.643931 3.4:-90178908 MS.gene012916:CDS
TAACAGCAGCAACAAACTTG+TGG 0.645143 3.4:+90179241 None:intergenic
TCGGAAGAAGAAGGACAATG+AGG 0.649373 3.4:-90179313 MS.gene012916:CDS
TGTTGAAAATATTACAAGGG+AGG 0.651126 3.4:-90178905 MS.gene012916:CDS
AAACAAAGACAAGTTTGACC+TGG 0.671629 3.4:-90179654 MS.gene012916:CDS
CTCCGATCATGTCAATACCA+CGG 0.699650 3.4:-90180297 MS.gene012916:intron
CGTGGTATTGACATGATCGG+AGG 0.711493 3.4:+90180298 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATAAATCAAATAAAAT+TGG + chr3.4:90179012-90179031 None:intergenic 10.0%
!! TAAAAAATTTATAACAACAA+AGG + chr3.4:90179803-90179822 None:intergenic 10.0%
!! TGAATAAATCAAATAAAATT+GGG + chr3.4:90179011-90179030 None:intergenic 10.0%
!!! ATATTTATGTTATTTATGTA+TGG - chr3.4:90179524-90179543 MS.gene012916:intron 10.0%
!! GTAAAATGAGAAATAAAATT+CGG + chr3.4:90180128-90180147 None:intergenic 15.0%
!!! TATGTTATTTATACACATTT+TGG - chr3.4:90179404-90179423 MS.gene012916:CDS 15.0%
!! AATAAAAATTGTTTGTGTTG+CGG + chr3.4:90180081-90180100 None:intergenic 20.0%
!! GAATGTTGAAAATATTACAA+GGG - chr3.4:90180352-90180371 MS.gene012916:CDS 20.0%
!! GTTATTAATCAATGATTGTT+TGG - chr3.4:90179773-90179792 MS.gene012916:intron 20.0%
!! TGAAAAAAACTTATGATGAT+AGG - chr3.4:90180187-90180206 MS.gene012916:intron 20.0%
!! TGAATGTTGAAAATATTACA+AGG - chr3.4:90180351-90180370 MS.gene012916:CDS 20.0%
!! TGATCATAATTGATAAGTAA+AGG - chr3.4:90179065-90179084 MS.gene012916:intron 20.0%
!!! TAGATATGATTTTAGTTTGT+TGG - chr3.4:90179130-90179149 MS.gene012916:intron 20.0%
!!! TATGTTATTTATGTATGGTT+GGG - chr3.4:90179529-90179548 MS.gene012916:intron 20.0%
!!! TTATGTTATTTATGTATGGT+TGG - chr3.4:90179528-90179547 MS.gene012916:intron 20.0%
!!! TTTAATTTATTTTCAATCCG+AGG - chr3.4:90180163-90180182 MS.gene012916:intron 20.0%
! ATTGTTCCTATACTATAACA+AGG + chr3.4:90179477-90179496 None:intergenic 25.0%
! CACATTTATAAACACTATAC+AGG + chr3.4:90179250-90179269 None:intergenic 25.0%
! CATGCATTATACACATAAAT+TGG + chr3.4:90179044-90179063 None:intergenic 25.0%
! GAACTTACTTTGAATTCATT+TGG - chr3.4:90179298-90179317 MS.gene012916:CDS 25.0%
! TATGTATATGTAGAGAGATA+CGG - chr3.4:90179089-90179108 MS.gene012916:intron 25.0%
! TCATGTTAATGTTGTATTAC+TGG - chr3.4:90180295-90180314 MS.gene012916:intron 25.0%
!! ATGTTATTTATGTATGGTTG+GGG - chr3.4:90179530-90179549 MS.gene012916:intron 25.0%
!!! TCATAAGTTTTTTTCAACCT+CGG + chr3.4:90180183-90180202 None:intergenic 25.0%
CTTTGAATTCATTTGGACTT+TGG - chr3.4:90179305-90179324 MS.gene012916:CDS 30.0%
TAATGAAACACTTCATGACA+TGG + chr3.4:90180258-90180277 None:intergenic 30.0%
TGTTGAAAATATTACAAGGG+AGG - chr3.4:90180355-90180374 MS.gene012916:CDS 30.0%
! ATCAAACCAAGAATCTTTTC+GGG + chr3.4:90180332-90180351 None:intergenic 30.0%
! CAAACTCCTTGTTATAGTAT+AGG - chr3.4:90179468-90179487 MS.gene012916:intron 30.0%
! CATCAAACCAAGAATCTTTT+CGG + chr3.4:90180333-90180352 None:intergenic 30.0%
AAACAAAGACAAGTTTGACC+TGG - chr3.4:90179606-90179625 MS.gene012916:CDS 35.0%
AACAAGGAGTTTGTGAAACT+AGG + chr3.4:90179461-90179480 None:intergenic 35.0%
AACAGCTAGTGAAGCAAAAA+CGG - chr3.4:90179564-90179583 MS.gene012916:intron 35.0%
CTATAATGAGCTAGTGATGA+AGG - chr3.4:90179845-90179864 MS.gene012916:intron 35.0%
GAAATAAAATTCGGTCAAGC+TGG + chr3.4:90180119-90180138 None:intergenic 35.0%
TAATGTTGTATTACTGGCAG+AGG - chr3.4:90180301-90180320 MS.gene012916:CDS 35.0%
TGCTGATAAAAAGTTTGACC+TGG - chr3.4:90179366-90179385 MS.gene012916:CDS 35.0%
TTCTAAATCCGAGAGATAAG+TGG + chr3.4:90178937-90178956 None:intergenic 35.0%
! GTTTGTTGGAAATTCTGCTT+CGG - chr3.4:90179144-90179163 MS.gene012916:intron 35.0%
! TTATGATGATAGGTGCTACA+TGG - chr3.4:90180197-90180216 MS.gene012916:intron 35.0%
AAAAGAAAAGAAGCAGACCG+TGG + chr3.4:90178983-90179002 None:intergenic 40.0%
AAATATTACAAGGGAGGACG+TGG - chr3.4:90180361-90180380 MS.gene012916:CDS 40.0%
AATTAACCACCTCAAAGCCA+AGG + chr3.4:90179204-90179223 None:intergenic 40.0%
ACTTAAGCTGCTTGAACTTG+TGG - chr3.4:90179663-90179682 MS.gene012916:CDS 40.0%
AGGTTTCACCAGAAATTCAG+AGG + chr3.4:90179886-90179905 None:intergenic 40.0%
ATAAAATTCGGTCAAGCTGG+AGG + chr3.4:90180116-90180135 None:intergenic 40.0%
ATACCATAACAGCGATTACC+AGG + chr3.4:90179387-90179406 None:intergenic 40.0%
CAGGTCATATCTAAGCAATG+TGG + chr3.4:90179231-90179250 None:intergenic 40.0%
CCATTTGGCAGCATGTAATT+CGG - chr3.4:90179928-90179947 MS.gene012916:intron 40.0%
GAACTGAACTTACTTACGAC+CGG + chr3.4:90179706-90179725 None:intergenic 40.0%
GATCAAAGACTCCTAAGTCA+TGG - chr3.4:90179426-90179445 MS.gene012916:CDS 40.0%
GCATGTAATTCGGAAGAAGA+AGG - chr3.4:90179938-90179957 MS.gene012916:intron 40.0%
GGTCAAACATACCATGACTT+AGG + chr3.4:90179440-90179459 None:intergenic 40.0%
GTTGCTGCTGTTAATCAGTT+AGG - chr3.4:90180029-90180048 MS.gene012916:intron 40.0%
TAACAGCAGCAACAAACTTG+TGG + chr3.4:90180022-90180041 None:intergenic 40.0%
TAGAGCAGCTAAAGACCATT+TGG - chr3.4:90179913-90179932 MS.gene012916:intron 40.0%
TCAAGCTGGAGGAAATTGAT+AGG + chr3.4:90180105-90180124 None:intergenic 40.0%
TGAACTTGTGGAACTTGAGA+CGG - chr3.4:90179675-90179694 MS.gene012916:CDS 40.0%
TTTAGCTGCTCTAATCTCAC+AGG + chr3.4:90179906-90179925 None:intergenic 40.0%
!!! GTCTTTGTTTTCTCGCAACA+TGG + chr3.4:90179596-90179615 None:intergenic 40.0%
AGAAGAAGTCCCAGTCTGTT+TGG - chr3.4:90179982-90180001 MS.gene012916:intron 45.0%
AGCTTAAGTCCATCCATGTC+AGG + chr3.4:90179652-90179671 None:intergenic 45.0%
CCGAATTACATGCTGCCAAA+TGG + chr3.4:90179931-90179950 None:intergenic 45.0%
CTCCGATCATGTCAATACCA+CGG - chr3.4:90178963-90178982 MS.gene012916:intron 45.0%
GCTGTACCCGAAAAGATTCT+TGG - chr3.4:90180323-90180342 MS.gene012916:CDS 45.0%
TCGGAAGAAGAAGGACAATG+AGG - chr3.4:90179947-90179966 MS.gene012916:intron 45.0%
TGACCTGGTAATCGCTGTTA+TGG - chr3.4:90179381-90179400 MS.gene012916:CDS 45.0%
! GTGATGAAGGCAGTTTTCCA+TGG - chr3.4:90179858-90179877 MS.gene012916:intron 45.0%
!! GACCGTGGTATTGACATGAT+CGG + chr3.4:90178968-90178987 None:intergenic 45.0%
AGGCAGCAAATGCCGCAATT+AGG + chr3.4:90179184-90179203 None:intergenic 50.0%
ATAAGTGGAATCGCCGTCGA+CGG + chr3.4:90178922-90178941 None:intergenic 50.0%
CAGCAAATGCCGCAATTAGG+CGG + chr3.4:90179181-90179200 None:intergenic 50.0%
CCATGGTGCCTCTGAATTTC+TGG - chr3.4:90179875-90179894 MS.gene012916:intron 50.0%
CGGCGATTCCACTTATCTCT+CGG - chr3.4:90178926-90178945 MS.gene012916:CDS 50.0%
CTCCACAGACCAAACAGACT+GGG + chr3.4:90179994-90180013 None:intergenic 50.0%
GGGACTTCTTCTGAGCTGAT+AGG + chr3.4:90179974-90179993 None:intergenic 50.0%
TAAGTCCATCCATGTCAGGC+AGG + chr3.4:90179648-90179667 None:intergenic 50.0%
TGAACTTACTTACGACCGGC+AGG + chr3.4:90179702-90179721 None:intergenic 50.0%
TGTTTGTGTTGCGGTCAGAG+TGG + chr3.4:90180072-90180091 None:intergenic 50.0%
! AATTGCGGCATTTGCTGCCT+TGG - chr3.4:90179184-90179203 MS.gene012916:intron 50.0%
!! CATTTGCTGCCTTGGCTTTG+AGG - chr3.4:90179192-90179211 MS.gene012916:intron 50.0%
!! CCAGAAATTCAGAGGCACCA+TGG + chr3.4:90179878-90179897 None:intergenic 50.0%
!! CGTGGTATTGACATGATCGG+AGG + chr3.4:90178965-90178984 None:intergenic 50.0%
AATGCCGCAATTAGGCGGCA+AGG + chr3.4:90179176-90179195 None:intergenic 55.0%
ATGCCGCAATTAGGCGGCAA+GGG + chr3.4:90179175-90179194 None:intergenic 55.0%
CACGCATTCCGATGGGAAAC+CGG + chr3.4:90178893-90178912 None:intergenic 55.0%
GCGAGAACACGCATTCCGAT+GGG + chr3.4:90178900-90178919 None:intergenic 55.0%
GCTCCACAGACCAAACAGAC+TGG + chr3.4:90179995-90180014 None:intergenic 55.0%
GGTGCACATCAGCGATTACC+AGG + chr3.4:90179627-90179646 None:intergenic 55.0%
GTCCCAGTCTGTTTGGTCTG+TGG - chr3.4:90179989-90180008 MS.gene012916:intron 55.0%
TGATGTGCACCTGCCTGACA+TGG - chr3.4:90179636-90179655 MS.gene012916:CDS 55.0%
! TCCAGTGACCGGTTTCCCAT+CGG - chr3.4:90178882-90178901 MS.gene012916:CDS 55.0%
!! TTGCTGCCTTGGCTTTGAGG+TGG - chr3.4:90179195-90179214 MS.gene012916:intron 55.0%
AGGACGTGGCAAGCCATCTC+CGG - chr3.4:90180375-90180394 MS.gene012916:CDS 60.0%
ATGCGTGTTCTCGCCGTCGA+CGG - chr3.4:90178906-90178925 MS.gene012916:CDS 60.0%
CGCAATTAGGCGGCAAGGGA+CGG + chr3.4:90179171-90179190 None:intergenic 60.0%
CGTCCCTTGCCGCCTAATTG+CGG - chr3.4:90179169-90179188 MS.gene012916:intron 60.0%
GGAACTTGAGACGGACCTGC+CGG - chr3.4:90179684-90179703 MS.gene012916:CDS 60.0%
GGCGAGAACACGCATTCCGA+TGG + chr3.4:90178901-90178920 None:intergenic 60.0%
GTGCACCTGCCTGACATGGA+TGG - chr3.4:90179640-90179659 MS.gene012916:CDS 60.0%
TCCGATGGGAAACCGGTCAC+TGG + chr3.4:90178886-90178905 None:intergenic 60.0%
GGACGTGGCAAGCCATCTCC+GGG - chr3.4:90180376-90180395 MS.gene012916:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 90178873 90180409 90178873 ID=MS.gene012916
chr3.4 mRNA 90178873 90180409 90178873 ID=MS.gene012916.t1;Parent=MS.gene012916
chr3.4 exon 90180298 90180409 90180298 ID=MS.gene012916.t1.exon1;Parent=MS.gene012916.t1
chr3.4 CDS 90180298 90180409 90180298 ID=cds.MS.gene012916.t1;Parent=MS.gene012916.t1
chr3.4 exon 90179570 90179722 90179570 ID=MS.gene012916.t1.exon2;Parent=MS.gene012916.t1
chr3.4 CDS 90179570 90179722 90179570 ID=cds.MS.gene012916.t1;Parent=MS.gene012916.t1
chr3.4 exon 90179223 90179453 90179223 ID=MS.gene012916.t1.exon3;Parent=MS.gene012916.t1
chr3.4 CDS 90179223 90179453 90179223 ID=cds.MS.gene012916.t1;Parent=MS.gene012916.t1
chr3.4 exon 90178873 90178961 90178873 ID=MS.gene012916.t1.exon4;Parent=MS.gene012916.t1
chr3.4 CDS 90178873 90178961 90178873 ID=cds.MS.gene012916.t1;Parent=MS.gene012916.t1
Gene Sequence

>MS.gene012916

ATGGATGACTCCAGTGACCGGTTTCCCATCGGAATGCGTGTTCTCGCCGTCGACGGCGATTCCACTTATCTCTCGGATTTAGAAACTCGCCTCCGATCATGTCAATACCACGGTCTGCTTCTTTTCTTTTTTATACCCAATTTTATTTGATTTATTCATACATTATGTCCAATTTATGTGTATAATGCATGATGATCATAATTGATAAGTAAAGGATATGTATATGTAGAGAGATACGGTTAATTCTTTTATTGATGTAGATATGATTTTAGTTTGTTGGAAATTCTGCTTCGGTCCGTCCCTTGCCGCCTAATTGCGGCATTTGCTGCCTTGGCTTTGAGGTGGTTAATTTAGTCCACATTGCTTAGATATGACCTGTATAGTGTTTATAAATGTGTAATTGCAATTATAGAAATTCAAACAAAGAACTTACTTTGAATTCATTTGGACTTTGGAATCAATCAAATCTAACTGCGAAATCAAAAAGATGTTGTGCTGATAAAAAGTTTGACCTGGTAATCGCTGTTATGGTATGTTATTTATACACATTTTGGATCAAAGACTCCTAAGTCATGGTATGTTTGACCTAGTTTCACAAACTCCTTGTTATAGTATAGGAACAATATATACACATTTTGCTTGTTCTGAACGATATTTATGTTATTTATGTATGGTTGGGGATTGCAGTAACAACAGCTAGTGAAGCAAAAACGGCATTGACCATGTTGCGAGAAAACAAAGACAAGTTTGACCTGGTAATCGCTGATGTGCACCTGCCTGACATGGATGGACTTAAGCTGCTTGAACTTGTGGAACTTGAGACGGACCTGCCGGTCGTAAGTAAGTTCAGTTCTTCATCTTTGTTTTGTTTCTTCATTTTCACTCTCATTTATTCATATAGTTATTAATCAATGATTGTTTGGTGAACCTTTGTTGTTATAAATTTTTTATTTCAGTGTTTTCTGAATATAGCTATAATGAGCTAGTGATGAAGGCAGTTTTCCATGGTGCCTCTGAATTTCTGGTGAAACCTGTGAGATTAGAGCAGCTAAAGACCATTTGGCAGCATGTAATTCGGAAGAAGAAGGACAATGAGGACCTATCAGCTCAGAAGAAGTCCCAGTCTGTTTGGTCTGTGGAGCTGCACCACAAGTTTGTTGCTGCTGTTAATCAGTTAGGCATTGACAGTATGTTCTCCACTCTGACCGCAACACAAACAATTTTTATTTCCTATCAATTTCCTCCAGCTTGACCGAATTTTATTTCTCATTTTACATTATCAAAGTGTTTTTTAATTTATTTTCAATCCGAGGTTGAAAAAAACTTATGATGATAGGTGCTACATGGTATAAATTTCATTTCTATCATCACTCAAATTTATGCCATGTCATGAAGTGTTTCATTACTGAATTTGAGTGAGTCTCATGTTAATGTTGTATTACTGGCAGAGGCTGTACCCGAAAAGATTCTTGGTTTGATGAATGTTGAAAATATTACAAGGGAGGACGTGGCAAGCCATCTCCGGGCAATTTTCTAG
Protein sequence

>MS.gene012916.t1

MDDSSDRFPIGMRVLAVDGDSTYLSDLETRLRSCQYHVTTASEAKTALTMLRENKDKFDLVIADVHLPDMDGLKLLELVELETDLPVVMFSEYSYNELVMKAVFHGASEFLVKPVRLEQLKTIWQHVIRKKKDNEDLSAQKKSQSVWSVELHHKFVAAVNQLGIDKAVPEKILGLMNVENITREDVASHLRAIF