Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013067.t1 | XP_003602916.2 | 94.4 | 478 | 27 | 0 | 1 | 478 | 3 | 480 | 1.20E-239 | 839 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013067.t1 | G7J542 | 94.4 | 478 | 27 | 0 | 1 | 478 | 3 | 480 | 8.5e-240 | 839.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene013067.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013067.t1 | MTR_3g100350 | 96.862 | 478 | 15 | 0 | 1 | 478 | 3 | 480 | 0.0 | 960 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013067.t1 | AT2G19260 | 47.297 | 222 | 90 | 5 | 259 | 478 | 410 | 606 | 1.65e-54 | 193 |
| MS.gene013067.t1 | AT2G19260 | 47.297 | 222 | 90 | 5 | 259 | 478 | 410 | 606 | 1.65e-54 | 193 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCCTGTATACCAAATT+AGG | 0.145112 | 3.3:-83309940 | None:intergenic |
| CTGCCTTCTGTCTTTCTTGA+TGG | 0.157712 | 3.3:+83310135 | MS.gene013067:CDS |
| GTCATTCATGTCTTAATAAA+AGG | 0.164007 | 3.3:+83310583 | MS.gene013067:CDS |
| TGAAAACACTGCACCTAATT+TGG | 0.214850 | 3.3:+83309927 | MS.gene013067:CDS |
| ATTTCAATTTGATGCTTAAC+TGG | 0.257269 | 3.3:-83310096 | None:intergenic |
| CCTCTTCCAATCCCGCCTTA+TGG | 0.261747 | 3.3:+83316724 | MS.gene013067:CDS |
| CTCTTCCAATCCCGCCTTAT+GGG | 0.272794 | 3.3:+83316725 | MS.gene013067:CDS |
| GGATTAATTTGCAATGGTTC+TGG | 0.279634 | 3.3:-83311687 | None:intergenic |
| CCACGTATCAACAGTGGAAT+AGG | 0.299158 | 3.3:+83310639 | MS.gene013067:CDS |
| TTCCTCTTCAAAGTCAAATA+TGG | 0.306991 | 3.3:+83310215 | MS.gene013067:CDS |
| GTCATTATGCTTGCAGTTGA+TGG | 0.308475 | 3.3:-83309904 | None:intergenic |
| GGGAAATTATATGTGGAAAA+TGG | 0.312495 | 3.3:+83311899 | MS.gene013067:CDS |
| ATCTCTCAACATCAACAGTA+TGG | 0.312758 | 3.3:-83310700 | None:intergenic |
| ACTTGTGAATGAAGTAAAAT+TGG | 0.312843 | 3.3:-83310028 | None:intergenic |
| ACTGGTCAGGCCCTGTAAAA+AGG | 0.314665 | 3.3:+83310781 | MS.gene013067:CDS |
| ATGCAAAATATGTGGTCATT+TGG | 0.356290 | 3.3:+83310458 | MS.gene013067:CDS |
| AACCAATGGAAGAGGATAAT+AGG | 0.372313 | 3.3:+83309833 | MS.gene013067:CDS |
| ACTCGCACACCAGTCGTATA+TGG | 0.376421 | 3.3:-83310729 | None:intergenic |
| GGAGTCCATTACTATTAAAC+TGG | 0.383396 | 3.3:-83310295 | None:intergenic |
| CCGTTAATAACTGCTGTTCC+AGG | 0.393854 | 3.3:+83310433 | MS.gene013067:CDS |
| AGAAGCCATGGACTTCTTAG+AGG | 0.397484 | 3.3:+83310363 | MS.gene013067:CDS |
| TTGAAGTTCAAACCGATGAC+TGG | 0.411899 | 3.3:+83315531 | MS.gene013067:CDS |
| CTCCTCCAGTTTAATAGTAA+TGG | 0.416267 | 3.3:+83310290 | MS.gene013067:CDS |
| GACTCAGTTCCATTGGCAAC+TGG | 0.419564 | 3.3:+83311845 | MS.gene013067:CDS |
| TGCTGATTGTGCTGTACCTC+AGG | 0.420947 | 3.3:+83315589 | MS.gene013067:CDS |
| CTTCTTAGTTCAGTTGAAGC+TGG | 0.423834 | 3.3:+83309691 | MS.gene013067:CDS |
| ATCTAGAACTGTCTCAGTTA+AGG | 0.430840 | 3.3:+83310665 | MS.gene013067:CDS |
| TGACAAAACCACTCATCAAT+TGG | 0.432889 | 3.3:-83310564 | None:intergenic |
| TTCCTATTATCCTCTTCCAT+TGG | 0.446285 | 3.3:-83309835 | None:intergenic |
| CTGCACCTAATTTGGTATAC+AGG | 0.447123 | 3.3:+83309935 | MS.gene013067:CDS |
| TGGCAACTGGCTTCAATGCC+GGG | 0.448378 | 3.3:+83311858 | MS.gene013067:CDS |
| GCCTAGTGGTGAAACTTGTC+AGG | 0.449374 | 3.3:+83309762 | MS.gene013067:CDS |
| GCAACTGAAGTATATAGAAA+TGG | 0.449691 | 3.3:+83316402 | MS.gene013067:CDS |
| TTCTGTTAATTCTTCCCTTG+TGG | 0.451346 | 3.3:-83310316 | None:intergenic |
| TTCAAGCAGCAGTCCTTGAC+TGG | 0.453183 | 3.3:+83310763 | MS.gene013067:CDS |
| CCAATGGAACTGAGTCTAGT+TGG | 0.456915 | 3.3:-83311838 | None:intergenic |
| TTGGCAACTGGCTTCAATGC+CGG | 0.457404 | 3.3:+83311857 | MS.gene013067:CDS |
| TAACTGGAAAAGCAGCTGTT+TGG | 0.459703 | 3.3:-83310080 | None:intergenic |
| GGTGGTAACGGCATCTTCAA+TGG | 0.462759 | 3.3:-83310412 | None:intergenic |
| ACGTGCCCATAAGGCGGGAT+TGG | 0.464010 | 3.3:-83316730 | None:intergenic |
| GGGAGGTTATAGACAAAACT+AGG | 0.470852 | 3.3:+83311878 | MS.gene013067:CDS |
| CTTAGACAGTTTGAGTTGAA+AGG | 0.472980 | 3.3:-83309868 | None:intergenic |
| AGAGATAAGACGTGCCCATA+AGG | 0.481151 | 3.3:-83316739 | None:intergenic |
| GCAGCAGTCCTTGACTGGTC+AGG | 0.487944 | 3.3:+83310768 | MS.gene013067:CDS |
| CACGTATCAACAGTGGAATA+GGG | 0.490240 | 3.3:+83310640 | MS.gene013067:CDS |
| AAGGGAAGAATTAACAGAAA+AGG | 0.490860 | 3.3:+83310320 | MS.gene013067:CDS |
| CCTGGAACAGCAGTTATTAA+CGG | 0.498635 | 3.3:-83310433 | None:intergenic |
| GTCATCCATCTCAGTTGCTA+TGG | 0.501394 | 3.3:-83310253 | None:intergenic |
| CTATGTTATGTATATGATGA+TGG | 0.506451 | 3.3:+83309715 | MS.gene013067:CDS |
| ACTGGTGTGCGAGTTGGTAA+AGG | 0.509532 | 3.3:+83310738 | MS.gene013067:CDS |
| AAGTTTCACCACTAGGCATC+CGG | 0.511152 | 3.3:-83309756 | None:intergenic |
| CCATAAGGCGGGATTGGAAG+AGG | 0.521585 | 3.3:-83316724 | None:intergenic |
| TCTGAAGGATTAATTTGCAA+TGG | 0.523395 | 3.3:-83311693 | None:intergenic |
| TACCATATTTGACTTTGAAG+AGG | 0.529766 | 3.3:-83310217 | None:intergenic |
| TTCAGAAATTTATAGACCGC+AGG | 0.529919 | 3.3:+83311710 | MS.gene013067:CDS |
| AAACGTGCATTGTAGCAAGA+TGG | 0.530372 | 3.3:-83310531 | None:intergenic |
| ATAAGACGTGCCCATAAGGC+GGG | 0.530589 | 3.3:-83316735 | None:intergenic |
| TCGGTTTGAACTTCAAATAG+TGG | 0.539950 | 3.3:-83315524 | None:intergenic |
| AGAAAGACAGAAGGCAGAGT+AGG | 0.541798 | 3.3:-83310129 | None:intergenic |
| AAAAGCTGAACCAATGGAAG+AGG | 0.542617 | 3.3:+83309825 | MS.gene013067:CDS |
| AGATTCCATAGCAACTGAGA+TGG | 0.550596 | 3.3:+83310248 | MS.gene013067:CDS |
| TTTGCACTTGTCATCAGCTG+AGG | 0.553862 | 3.3:+83310056 | MS.gene013067:CDS |
| CCAACTAGACTCAGTTCCAT+TGG | 0.554372 | 3.3:+83311838 | MS.gene013067:CDS |
| AAAACTAGGGAAATTATATG+TGG | 0.554778 | 3.3:+83311892 | MS.gene013067:CDS |
| TTACAGGGCCTGACCAGTCA+AGG | 0.559672 | 3.3:-83310776 | None:intergenic |
| GCACTTGTCTGCACATCGGT+CGG | 0.559798 | 3.3:-83310006 | None:intergenic |
| GCAACAAGTACCCTGCTGAC+TGG | 0.560642 | 3.3:+83309797 | MS.gene013067:CDS |
| TTATATGTGGAAAATGGCGC+AGG | 0.561470 | 3.3:+83311905 | MS.gene013067:CDS |
| TGCGGTCTATAAATTTCTGA+AGG | 0.561834 | 3.3:-83311708 | None:intergenic |
| CATTACTATTAAACTGGAGG+AGG | 0.567120 | 3.3:-83310289 | None:intergenic |
| TGAAGTTCAAACCGATGACT+GGG | 0.570865 | 3.3:+83315532 | MS.gene013067:CDS |
| GCAGTTATTAACGGTGGTAA+CGG | 0.572035 | 3.3:-83310424 | None:intergenic |
| GTGTAGAAAAGCTGAACCAA+TGG | 0.583872 | 3.3:+83309819 | MS.gene013067:CDS |
| CCTATTCCACTGTTGATACG+TGG | 0.585601 | 3.3:-83310639 | None:intergenic |
| GCATTGAAGCCAGTTGCCAA+TGG | 0.585878 | 3.3:-83311854 | None:intergenic |
| TCTCTCAACATCAACAGTAT+GGG | 0.587204 | 3.3:-83310699 | None:intergenic |
| ACAGAAGCACTCCCAGTCAT+CGG | 0.588116 | 3.3:-83315543 | None:intergenic |
| TAATAGTAATGGACTCCACA+AGG | 0.589290 | 3.3:+83310301 | MS.gene013067:CDS |
| TATACGACTGGTGTGCGAGT+TGG | 0.590103 | 3.3:+83310732 | MS.gene013067:CDS |
| TATGTTATGTATATGATGAT+GGG | 0.592983 | 3.3:+83309716 | MS.gene013067:CDS |
| TCCAGGTCATGCAAAATATG+TGG | 0.597837 | 3.3:+83310450 | MS.gene013067:CDS |
| AGATACTCTTACAGATAATG+TGG | 0.604361 | 3.3:+83310386 | MS.gene013067:CDS |
| GTCCATTACTATTAAACTGG+AGG | 0.605303 | 3.3:-83310292 | None:intergenic |
| ACAGCACAATCAGCATGAGA+TGG | 0.605642 | 3.3:-83315581 | None:intergenic |
| TATCTCCTCTAAGAAGTCCA+TGG | 0.605795 | 3.3:-83310368 | None:intergenic |
| AAAGAGTACCAATTGATGAG+TGG | 0.612414 | 3.3:+83310556 | MS.gene013067:CDS |
| ATTCCACTGTTGATACGTGG+TGG | 0.618449 | 3.3:-83310636 | None:intergenic |
| CAACGTGACCGGATGCCTAG+TGG | 0.622842 | 3.3:+83309748 | MS.gene013067:CDS |
| ACCTGACAAGTTTCACCACT+AGG | 0.624843 | 3.3:-83309763 | None:intergenic |
| GATAAGACGTGCCCATAAGG+CGG | 0.625500 | 3.3:-83316736 | None:intergenic |
| TCAAACTGTCTAAGAAGCTG+TGG | 0.626076 | 3.3:+83309877 | MS.gene013067:CDS |
| GATACTGAGCCATATACGAC+TGG | 0.630007 | 3.3:+83310720 | MS.gene013067:CDS |
| AGCTGATGACAAGTGCAAAG+AGG | 0.633110 | 3.3:-83310052 | None:intergenic |
| AAGGCGGGATTGGAAGAGGA+GGG | 0.636500 | 3.3:-83316720 | None:intergenic |
| TAAGGCGGGATTGGAAGAGG+AGG | 0.641496 | 3.3:-83316721 | None:intergenic |
| GGAGGTTATAGACAAAACTA+GGG | 0.658232 | 3.3:+83311879 | MS.gene013067:CDS |
| GCAGGAGGTGGAAACAGATG+AGG | 0.662977 | 3.3:+83316372 | MS.gene013067:intron |
| ACTCCATCAAGAAAGACAGA+AGG | 0.671873 | 3.3:-83310138 | None:intergenic |
| AAACCACCACGTATCAACAG+TGG | 0.676321 | 3.3:+83310633 | MS.gene013067:CDS |
| AATAGTAATGGACTCCACAA+GGG | 0.679473 | 3.3:+83310302 | MS.gene013067:CDS |
| ATTGGCACTTGTCTGCACAT+CGG | 0.680439 | 3.3:-83310010 | None:intergenic |
| AGGCGGGATTGGAAGAGGAG+GGG | 0.692067 | 3.3:-83316719 | None:intergenic |
| CAACTGGCTTCAATGCCGGG+AGG | 0.715328 | 3.3:+83311861 | MS.gene013067:CDS |
| GCAACTGAGATGGATGACAC+TGG | 0.735945 | 3.3:+83310258 | MS.gene013067:CDS |
| GGAACAGCAGTTATTAACGG+TGG | 0.741703 | 3.3:-83310430 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 83309679 | 83316776 | 83309679 | ID=MS.gene013067 |
| chr3.3 | mRNA | 83309679 | 83316776 | 83309679 | ID=MS.gene013067.t1;Parent=MS.gene013067 |
| chr3.3 | exon | 83309679 | 83310802 | 83309679 | ID=MS.gene013067.t1.exon1;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83309679 | 83310802 | 83309679 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| chr3.3 | exon | 83311671 | 83311731 | 83311671 | ID=MS.gene013067.t1.exon2;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83311671 | 83311731 | 83311671 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| chr3.3 | exon | 83311820 | 83311926 | 83311820 | ID=MS.gene013067.t1.exon3;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83311820 | 83311926 | 83311820 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| chr3.3 | exon | 83315520 | 83315610 | 83315520 | ID=MS.gene013067.t1.exon4;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83315520 | 83315610 | 83315520 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| chr3.3 | exon | 83316376 | 83316423 | 83316376 | ID=MS.gene013067.t1.exon5;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83316376 | 83316423 | 83316376 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| chr3.3 | exon | 83316708 | 83316776 | 83316708 | ID=MS.gene013067.t1.exon6;Parent=MS.gene013067.t1 |
| chr3.3 | CDS | 83316708 | 83316776 | 83316708 | ID=cds.MS.gene013067.t1;Parent=MS.gene013067.t1 |
| Gene Sequence |
| Protein sequence |
