Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013147.t1 | QDX15124.1 | 99.3 | 152 | 1 | 0 | 1 | 152 | 64 | 215 | 3.70E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013147.t1 | Q9SMP0 | 82.0 | 161 | 18 | 5 | 1 | 152 | 75 | 233 | 1.6e-61 | 236.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013147.t1 | A0A5B8HMF8 | 99.3 | 152 | 1 | 0 | 1 | 152 | 64 | 215 | 2.7e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene013147.t1 | TF | NF-YC |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013147.t1 | MTR_3g099180 | 98.693 | 153 | 0 | 2 | 1 | 152 | 65 | 216 | 1.72e-106 | 303 |
| MS.gene013147.t1 | MTR_3g099180 | 98.693 | 153 | 0 | 2 | 1 | 152 | 65 | 216 | 2.63e-106 | 302 |
| MS.gene013147.t1 | MTR_7g113680 | 63.077 | 130 | 36 | 4 | 1 | 120 | 162 | 289 | 5.13e-48 | 157 |
| MS.gene013147.t1 | MTR_1g082660 | 65.000 | 120 | 30 | 4 | 1 | 109 | 111 | 229 | 1.41e-45 | 150 |
| MS.gene013147.t1 | MTR_2g023340 | 46.053 | 152 | 62 | 4 | 1 | 134 | 95 | 244 | 4.19e-35 | 122 |
| MS.gene013147.t1 | MTR_2g081600 | 56.757 | 74 | 32 | 0 | 2 | 75 | 34 | 107 | 3.35e-24 | 91.3 |
| MS.gene013147.t1 | MTR_2g081630 | 58.571 | 70 | 29 | 0 | 2 | 71 | 34 | 103 | 1.09e-23 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013147.t1 | AT3G48590 | 85.106 | 141 | 14 | 3 | 1 | 135 | 75 | 214 | 1.39e-79 | 236 |
| MS.gene013147.t1 | AT5G63470 | 82.639 | 144 | 15 | 3 | 1 | 135 | 88 | 230 | 1.50e-77 | 231 |
| MS.gene013147.t1 | AT5G63470 | 82.639 | 144 | 15 | 3 | 1 | 135 | 88 | 230 | 1.50e-77 | 231 |
| MS.gene013147.t1 | AT1G54830 | 63.636 | 132 | 36 | 6 | 1 | 125 | 80 | 206 | 5.68e-48 | 155 |
| MS.gene013147.t1 | AT1G54830 | 63.636 | 132 | 36 | 6 | 1 | 125 | 80 | 206 | 5.68e-48 | 155 |
| MS.gene013147.t1 | AT1G54830 | 63.636 | 132 | 36 | 6 | 1 | 125 | 80 | 206 | 5.68e-48 | 155 |
| MS.gene013147.t1 | AT1G56170 | 66.972 | 109 | 31 | 2 | 1 | 107 | 86 | 191 | 4.05e-47 | 152 |
| MS.gene013147.t1 | AT1G56170 | 66.972 | 109 | 31 | 2 | 1 | 107 | 86 | 191 | 4.05e-47 | 152 |
| MS.gene013147.t1 | AT1G08970 | 68.103 | 116 | 27 | 4 | 1 | 107 | 90 | 204 | 5.73e-47 | 152 |
| MS.gene013147.t1 | AT1G08970 | 68.103 | 116 | 27 | 4 | 1 | 107 | 90 | 204 | 5.73e-47 | 152 |
| MS.gene013147.t1 | AT1G08970 | 68.103 | 116 | 27 | 4 | 1 | 107 | 90 | 204 | 5.73e-47 | 152 |
| MS.gene013147.t1 | AT1G08970 | 68.103 | 116 | 27 | 4 | 1 | 107 | 90 | 204 | 5.73e-47 | 152 |
| MS.gene013147.t1 | AT5G50480 | 52.518 | 139 | 52 | 6 | 1 | 135 | 64 | 192 | 4.37e-38 | 129 |
| MS.gene013147.t1 | AT5G27910 | 45.395 | 152 | 69 | 5 | 1 | 148 | 46 | 187 | 3.27e-32 | 114 |
| MS.gene013147.t1 | AT5G50490 | 48.214 | 112 | 53 | 2 | 1 | 109 | 46 | 155 | 1.08e-26 | 99.8 |
| MS.gene013147.t1 | AT5G50470 | 40.789 | 152 | 63 | 7 | 1 | 143 | 73 | 206 | 7.04e-26 | 98.2 |
| MS.gene013147.t1 | AT5G38140 | 45.588 | 68 | 37 | 0 | 1 | 68 | 77 | 144 | 8.50e-18 | 76.6 |
Find 41 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGGCTTGGCAGTCTGTT+TGG | 0.288988 | 3.3:+81707056 | MS.gene013147:CDS |
| TTGAACTCACCATTCGTTCT+TGG | 0.290380 | 3.3:+81706774 | MS.gene013147:CDS |
| TCCTTATTATTATCCTCCTA+TGG | 0.322109 | 3.3:+81706961 | MS.gene013147:CDS |
| CCCATAGGAGGATAATAATA+AGG | 0.322822 | 3.3:-81706962 | None:intergenic |
| CAAGCTTTGGCAAAGAGAAT+TGG | 0.395526 | 3.3:-81706737 | None:intergenic |
| GGTGCTGCTGCTGCTATTGT+TGG | 0.404432 | 3.3:+81706920 | MS.gene013147:CDS |
| TTATGTTCAGCCGCCTTCTC+AGG | 0.407879 | 3.3:+81707039 | MS.gene013147:CDS |
| AATGAAAAGCTCACAAGCTT+TGG | 0.414215 | 3.3:-81706750 | None:intergenic |
| TGGTCGTCCCGCTGTTGATC+CGG | 0.430949 | 3.3:+81707006 | MS.gene013147:CDS |
| CCTCCTATGGGACAACCTGC+TGG | 0.443243 | 3.3:+81706974 | MS.gene013147:CDS |
| TTCAGCCGCCTTCTCAGGCT+TGG | 0.445274 | 3.3:+81707044 | MS.gene013147:CDS |
| ATATTGCTGCTGCTATTACT+AGG | 0.451638 | 3.3:+81706840 | MS.gene013147:CDS |
| TGGCAGACGGGAGCGGATGA+TGG | 0.454235 | 3.3:+81707076 | MS.gene013147:CDS |
| TGGCAGTCTGTTTGGCAGAC+GGG | 0.457754 | 3.3:+81707064 | MS.gene013147:CDS |
| TCTTATGCTGGTGGTGGTGT+TGG | 0.460055 | 3.3:+81707100 | MS.gene013147:CDS |
| TTGGCAGTCTGTTTGGCAGA+CGG | 0.463047 | 3.3:+81707063 | MS.gene013147:CDS |
| GTCTGTTTGGCAGACGGGAG+CGG | 0.463208 | 3.3:+81707069 | MS.gene013147:CDS |
| CCCGCTGTTGATCCGGCTAC+CGG | 0.469158 | 3.3:+81707013 | MS.gene013147:CDS |
| CCTTATTATTATCCTCCTAT+GGG | 0.475192 | 3.3:+81706962 | MS.gene013147:CDS |
| GCGGATGATGGTTCTTATGC+TGG | 0.494268 | 3.3:+81707088 | MS.gene013147:CDS |
| ATCGAACAAAATGATACCTC+TGG | 0.517463 | 3.3:-81707147 | None:intergenic |
| ATTCCAGCAGGTTGTCCCAT+AGG | 0.521379 | 3.3:-81706977 | None:intergenic |
| GCTGGTGGTGGTGTTGGCAG+TGG | 0.530864 | 3.3:+81707106 | MS.gene013147:CDS |
| CAACCTGCTGGAATGATGAT+TGG | 0.531059 | 3.3:+81706986 | MS.gene013147:CDS |
| TCATCTTTAATCTCGTCTCT+CGG | 0.549440 | 3.3:-81706896 | None:intergenic |
| TGAACATAAACACCGGTAGC+CGG | 0.556443 | 3.3:-81707025 | None:intergenic |
| ATTGTTGGTGCTGCTGCTAG+TGG | 0.559151 | 3.3:+81706935 | MS.gene013147:CDS |
| CGACCAATCATCATTCCAGC+AGG | 0.560161 | 3.3:-81706989 | None:intergenic |
| CCGGTAGCCGGATCAACAGC+GGG | 0.566961 | 3.3:-81707013 | None:intergenic |
| AGTGGTCAGCACAATGGCGA+TGG | 0.568390 | 3.3:+81707124 | MS.gene013147:CDS |
| GATGGTTCTTATGCTGGTGG+TGG | 0.575597 | 3.3:+81707094 | MS.gene013147:CDS |
| GACTGCCAAGCCTGAGAAGG+CGG | 0.581694 | 3.3:-81707049 | None:intergenic |
| AGCATGAAGCCAAGAACGAA+TGG | 0.590029 | 3.3:-81706783 | None:intergenic |
| AGAGACGAGATTAAAGATGA+AGG | 0.596434 | 3.3:+81706899 | MS.gene013147:CDS |
| CCAGCAGGTTGTCCCATAGG+AGG | 0.597127 | 3.3:-81706974 | None:intergenic |
| ACAGACTGCCAAGCCTGAGA+AGG | 0.599594 | 3.3:-81707052 | None:intergenic |
| AGCACAATGGCGATGGCCAG+AGG | 0.601116 | 3.3:+81707131 | MS.gene013147:CDS |
| ACCGGTAGCCGGATCAACAG+CGG | 0.640253 | 3.3:-81707014 | None:intergenic |
| GTTGGCAGTGGTCAGCACAA+TGG | 0.663712 | 3.3:+81707118 | MS.gene013147:CDS |
| GATGATGGTTCTTATGCTGG+TGG | 0.665141 | 3.3:+81707091 | MS.gene013147:CDS |
| AGGCGGCTGAACATAAACAC+CGG | 0.726448 | 3.3:-81707032 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GACTGATATTTTTGATTTTT+TGG | + | chr3.3:81706862-81706881 | MS.gene013147:CDS | 20.0% |
| CCTTATTATTATCCTCCTAT+GGG | + | chr3.3:81706962-81706981 | MS.gene013147:CDS | 30.0% | |
| TCCTTATTATTATCCTCCTA+TGG | + | chr3.3:81706961-81706980 | MS.gene013147:CDS | 30.0% | |
| AATGAAAAGCTCACAAGCTT+TGG | - | chr3.3:81706753-81706772 | None:intergenic | 35.0% | |
| AGAGACGAGATTAAAGATGA+AGG | + | chr3.3:81706899-81706918 | MS.gene013147:CDS | 35.0% | |
| ATATTGCTGCTGCTATTACT+AGG | + | chr3.3:81706840-81706859 | MS.gene013147:CDS | 35.0% | |
| ATCGAACAAAATGATACCTC+TGG | - | chr3.3:81707150-81707169 | None:intergenic | 35.0% | |
| CCCATAGGAGGATAATAATA+AGG | - | chr3.3:81706965-81706984 | None:intergenic | 35.0% | |
| TCATCTTTAATCTCGTCTCT+CGG | - | chr3.3:81706899-81706918 | None:intergenic | 35.0% | |
| TTGAACTCACCATTCGTTCT+TGG | + | chr3.3:81706774-81706793 | MS.gene013147:CDS | 40.0% | |
| ! | CAAGCTTTGGCAAAGAGAAT+TGG | - | chr3.3:81706740-81706759 | None:intergenic | 40.0% |
| AGCATGAAGCCAAGAACGAA+TGG | - | chr3.3:81706786-81706805 | None:intergenic | 45.0% | |
| CAACCTGCTGGAATGATGAT+TGG | + | chr3.3:81706986-81707005 | MS.gene013147:CDS | 45.0% | |
| TGAACATAAACACCGGTAGC+CGG | - | chr3.3:81707028-81707047 | None:intergenic | 45.0% | |
| ! | GATGATGGTTCTTATGCTGG+TGG | + | chr3.3:81707091-81707110 | MS.gene013147:CDS | 45.0% |
| AGGCGGCTGAACATAAACAC+CGG | - | chr3.3:81707035-81707054 | None:intergenic | 50.0% | |
| ATTCCAGCAGGTTGTCCCAT+AGG | - | chr3.3:81706980-81706999 | None:intergenic | 50.0% | |
| CGACCAATCATCATTCCAGC+AGG | - | chr3.3:81706992-81707011 | None:intergenic | 50.0% | |
| GATGGTTCTTATGCTGGTGG+TGG | + | chr3.3:81707094-81707113 | MS.gene013147:CDS | 50.0% | |
| TTATGTTCAGCCGCCTTCTC+AGG | + | chr3.3:81707039-81707058 | MS.gene013147:CDS | 50.0% | |
| TTGGCAGTCTGTTTGGCAGA+CGG | + | chr3.3:81707063-81707082 | MS.gene013147:CDS | 50.0% | |
| ! | GCGGATGATGGTTCTTATGC+TGG | + | chr3.3:81707088-81707107 | MS.gene013147:CDS | 50.0% |
| !! | ATTGTTGGTGCTGCTGCTAG+TGG | + | chr3.3:81706935-81706954 | MS.gene013147:CDS | 50.0% |
| !! | TCTTATGCTGGTGGTGGTGT+TGG | + | chr3.3:81707100-81707119 | MS.gene013147:CDS | 50.0% |
| ACAGACTGCCAAGCCTGAGA+AGG | - | chr3.3:81707055-81707074 | None:intergenic | 55.0% | |
| AGTGGTCAGCACAATGGCGA+TGG | + | chr3.3:81707124-81707143 | MS.gene013147:CDS | 55.0% | |
| CTCAGGCTTGGCAGTCTGTT+TGG | + | chr3.3:81707056-81707075 | MS.gene013147:CDS | 55.0% | |
| GTTGGCAGTGGTCAGCACAA+TGG | + | chr3.3:81707118-81707137 | MS.gene013147:CDS | 55.0% | |
| TGGCAGTCTGTTTGGCAGAC+GGG | + | chr3.3:81707064-81707083 | MS.gene013147:CDS | 55.0% | |
| !! | GGTGCTGCTGCTGCTATTGT+TGG | + | chr3.3:81706920-81706939 | MS.gene013147:CDS | 55.0% |
| ACCGGTAGCCGGATCAACAG+CGG | - | chr3.3:81707017-81707036 | None:intergenic | 60.0% | |
| AGCACAATGGCGATGGCCAG+AGG | + | chr3.3:81707131-81707150 | MS.gene013147:CDS | 60.0% | |
| CCAGCAGGTTGTCCCATAGG+AGG | - | chr3.3:81706977-81706996 | None:intergenic | 60.0% | |
| CCTCCTATGGGACAACCTGC+TGG | + | chr3.3:81706974-81706993 | MS.gene013147:CDS | 60.0% | |
| GACTGCCAAGCCTGAGAAGG+CGG | - | chr3.3:81707052-81707071 | None:intergenic | 60.0% | |
| GTCTGTTTGGCAGACGGGAG+CGG | + | chr3.3:81707069-81707088 | MS.gene013147:CDS | 60.0% | |
| TGGTCGTCCCGCTGTTGATC+CGG | + | chr3.3:81707006-81707025 | MS.gene013147:CDS | 60.0% | |
| TTCAGCCGCCTTCTCAGGCT+TGG | + | chr3.3:81707044-81707063 | MS.gene013147:CDS | 60.0% | |
| CCCGCTGTTGATCCGGCTAC+CGG | + | chr3.3:81707013-81707032 | MS.gene013147:CDS | 65.0% | |
| CCGGTAGCCGGATCAACAGC+GGG | - | chr3.3:81707016-81707035 | None:intergenic | 65.0% | |
| TGGCAGACGGGAGCGGATGA+TGG | + | chr3.3:81707076-81707095 | MS.gene013147:CDS | 65.0% | |
| !! | GCTGGTGGTGGTGTTGGCAG+TGG | + | chr3.3:81707106-81707125 | MS.gene013147:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 81706695 | 81707177 | 81706695 | ID=MS.gene013147 |
| chr3.3 | mRNA | 81706695 | 81707177 | 81706695 | ID=MS.gene013147.t1;Parent=MS.gene013147 |
| chr3.3 | exon | 81706695 | 81707177 | 81706695 | ID=MS.gene013147.t1.exon1;Parent=MS.gene013147.t1 |
| chr3.3 | CDS | 81706695 | 81707177 | 81706695 | ID=cds.MS.gene013147.t1;Parent=MS.gene013147.t1 |
| Gene Sequence |
| Protein sequence |
