AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013182

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013182.t1 MTR_3g498825 98.350 303 3 2 1 302 1 302 0.0 607
MS.gene013182.t1 MTR_8g099880 59.032 310 94 11 1 295 1 292 2.26e-95 285
MS.gene013182.t1 MTR_1g017350 60.000 125 41 2 121 239 228 349 2.19e-43 154
MS.gene013182.t1 MTR_1g017350 60.000 125 41 2 121 239 228 349 3.10e-43 153
MS.gene013182.t1 MTR_3g116770 63.200 125 30 1 111 219 139 263 7.05e-43 152
MS.gene013182.t1 MTR_3g116770 63.200 125 30 1 111 219 139 263 7.51e-43 152
MS.gene013182.t1 MTR_7g053410 57.516 153 45 3 86 218 236 388 1.27e-42 153
MS.gene013182.t1 MTR_1g017350 61.667 120 37 2 121 234 228 344 1.55e-42 151
MS.gene013182.t1 MTR_8g065740 69.091 110 31 1 113 219 268 377 1.87e-42 152
MS.gene013182.t1 MTR_8g012290 62.500 136 40 4 109 236 245 377 2.18e-42 152
MS.gene013182.t1 MTR_8g012290 62.500 136 40 4 109 236 245 377 2.26e-42 152
MS.gene013182.t1 MTR_7g053410 57.516 153 45 3 86 218 236 388 2.29e-42 153
MS.gene013182.t1 MTR_1g017350 61.667 120 37 2 121 234 228 344 2.36e-42 151
MS.gene013182.t1 MTR_5g037250 61.475 122 45 2 98 218 63 183 3.16e-42 145
MS.gene013182.t1 MTR_5g048860 56.738 141 49 2 91 219 297 437 4.21e-41 150
MS.gene013182.t1 MTR_4g070320 70.642 109 28 3 113 219 109 215 2.32e-40 144
MS.gene013182.t1 MTR_8g062240 86.667 75 10 0 144 218 148 222 8.05e-40 140
MS.gene013182.t1 MTR_1g059270 55.652 115 42 2 144 252 157 268 2.23e-36 133
MS.gene013182.t1 MTR_1g059270 65.217 92 29 1 144 235 157 245 2.84e-36 130
MS.gene013182.t1 MTR_7g092510 68.889 90 25 1 144 233 170 256 2.15e-35 130
MS.gene013182.t1 MTR_6g084120 50.000 124 33 5 112 231 134 232 6.10e-27 106
MS.gene013182.t1 MTR_4g131160 41.722 151 71 4 147 293 196 333 1.86e-23 99.4
MS.gene013182.t1 MTR_4g131160 41.722 151 71 4 147 293 199 336 2.31e-23 99.4
MS.gene013182.t1 MTR_1g080890 61.039 77 29 1 136 212 182 257 1.56e-21 93.6
MS.gene013182.t1 MTR_4g033295 60.000 65 26 0 147 211 118 182 6.35e-18 80.5
MS.gene013182.t1 MTR_4g033295 58.209 67 27 1 147 212 118 184 9.08e-18 79.7
MS.gene013182.t1 MTR_4g065870 61.538 65 25 0 147 211 355 419 5.56e-17 81.3
MS.gene013182.t1 MTR_4g065870 61.290 62 24 0 147 208 355 416 2.79e-16 79.0
MS.gene013182.t1 MTR_1g069155 40.952 105 60 2 118 221 287 390 4.22e-16 79.0
MS.gene013182.t1 MTR_1g052470 50.633 79 39 0 147 225 298 376 6.61e-16 77.8
MS.gene013182.t1 MTR_2g098310 56.923 65 28 0 147 211 332 396 1.41e-15 77.0
MS.gene013182.t1 MTR_1g070870 40.909 132 72 2 110 236 78 208 2.06e-15 75.1
MS.gene013182.t1 MTR_7g092540 53.731 67 31 0 147 213 243 309 4.53e-15 74.7
MS.gene013182.t1 MTR_2g098310 56.061 66 29 0 145 210 330 395 4.64e-15 75.5
MS.gene013182.t1 MTR_1g070870 48.980 98 49 1 139 236 115 211 6.34e-15 73.9
MS.gene013182.t1 MTR_1g084980 37.273 110 64 3 107 212 418 526 8.13e-15 75.1
MS.gene013182.t1 MTR_7g092540 54.688 64 29 0 147 210 243 306 8.62e-15 73.9
MS.gene013182.t1 MTR_3g028520 53.846 65 30 0 147 211 322 386 4.01e-14 72.8
MS.gene013182.t1 MTR_3g027650 53.846 65 30 0 147 211 322 386 4.01e-14 72.8
MS.gene013182.t1 MTR_3g027650 54.839 62 28 0 147 208 322 383 1.73e-13 70.9
MS.gene013182.t1 MTR_7g099540 48.611 72 36 1 150 221 255 325 2.77e-13 70.1
MS.gene013182.t1 MTR_5g017040 37.391 115 67 3 112 222 67 180 1.48e-12 67.8
MS.gene013182.t1 MTR_3g449770 47.945 73 36 2 149 221 311 381 2.34e-12 67.4
MS.gene013182.t1 MTR_8g036235 49.231 65 33 0 147 211 316 380 2.52e-12 67.0
MS.gene013182.t1 MTR_1g019240 39.130 92 54 2 149 239 87 177 6.18e-12 65.1
MS.gene013182.t1 MTR_1g018090 53.125 64 29 1 148 211 275 337 8.60e-12 65.5
MS.gene013182.t1 MTR_4g088710 96.552 29 1 0 275 303 9 37 1.32e-11 59.7
MS.gene013182.t1 MTR_1g116790 50.000 64 32 0 147 210 265 328 1.69e-11 64.3
MS.gene013182.t1 MTR_2g098310 43.750 96 46 3 147 235 332 426 3.58e-11 63.9
MS.gene013182.t1 MTR_7g099540 44.872 78 36 2 150 221 255 331 4.56e-11 63.5
MS.gene013182.t1 MTR_3g103030 48.052 77 38 2 148 223 230 305 5.12e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013182.t1 AT5G50915 48.069 233 97 6 74 299 57 272 3.32e-59 191
MS.gene013182.t1 AT5G50915 48.069 233 97 6 74 299 57 272 3.32e-59 191
MS.gene013182.t1 AT5G50915 50.228 219 82 6 74 286 57 254 4.17e-59 190
MS.gene013182.t1 AT5G50915 50.228 219 82 6 74 286 57 254 4.17e-59 190
MS.gene013182.t1 AT1G68920 57.718 149 59 2 86 234 243 387 1.26e-46 164
MS.gene013182.t1 AT1G68920 57.718 149 59 2 86 234 243 387 1.26e-46 164
MS.gene013182.t1 AT1G68920 57.718 149 59 2 86 234 243 387 1.26e-46 164
MS.gene013182.t1 AT1G68920 56.209 153 54 2 92 234 237 386 1.36e-46 163
MS.gene013182.t1 AT1G59640 68.966 116 31 2 108 218 92 207 9.32e-45 153
MS.gene013182.t1 AT1G10120 60.976 123 45 1 112 234 171 290 1.01e-44 156
MS.gene013182.t1 AT1G10120 60.976 123 45 1 112 234 171 290 1.01e-44 156
MS.gene013182.t1 AT3G07340 65.079 126 43 1 93 218 205 329 2.62e-44 156
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 90 256 3.47e-44 154
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 119 285 4.44e-44 154
MS.gene013182.t1 AT3G07340 65.079 126 43 1 93 218 205 329 4.84e-44 156
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 141 307 6.70e-44 154
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 141 307 6.70e-44 154
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 141 307 6.70e-44 154
MS.gene013182.t1 AT1G26260 52.353 170 49 4 94 234 141 307 6.70e-44 154
MS.gene013182.t1 AT1G59640 68.966 116 31 2 108 218 92 207 1.62e-43 152
MS.gene013182.t1 AT5G48560 83.146 89 15 0 139 227 293 381 2.00e-43 155
MS.gene013182.t1 AT5G48560 83.146 89 15 0 139 227 293 381 3.68e-43 154
MS.gene013182.t1 AT3G23690 60.000 135 41 4 112 234 146 279 5.80e-42 149
MS.gene013182.t1 AT4G34530 87.179 78 10 0 142 219 167 244 3.29e-41 146
MS.gene013182.t1 AT5G62610 71.910 89 25 0 130 218 136 224 7.48e-39 138
MS.gene013182.t1 AT5G62610 71.910 89 25 0 130 218 136 224 7.48e-39 138
MS.gene013182.t1 AT4G36540 57.143 133 46 3 111 235 97 226 2.61e-38 137
MS.gene013182.t1 AT4G36540 57.143 133 46 3 111 235 97 226 2.82e-38 137
MS.gene013182.t1 AT2G18300 49.057 159 70 4 85 243 131 278 7.29e-38 137
MS.gene013182.t1 AT2G18300 55.556 135 52 3 85 219 131 257 1.50e-37 136
MS.gene013182.t1 AT2G18300 55.556 135 52 3 85 219 131 257 2.49e-37 136
MS.gene013182.t1 AT2G42300 64.583 96 31 1 144 239 182 274 6.84e-37 133
MS.gene013182.t1 AT1G18400 71.264 87 24 1 145 231 143 228 7.68e-37 132
MS.gene013182.t1 AT3G57800 71.111 90 23 1 144 233 201 287 9.49e-37 135
MS.gene013182.t1 AT1G18400 71.264 87 24 1 145 231 143 228 1.12e-36 132
MS.gene013182.t1 AT1G73830 68.478 92 28 1 146 237 146 236 4.43e-36 130
MS.gene013182.t1 AT1G73830 70.930 86 24 1 146 231 146 230 9.22e-36 129
MS.gene013182.t1 AT2G42300 66.304 92 28 1 144 235 182 270 1.26e-35 131
MS.gene013182.t1 AT1G25330 52.518 139 54 4 99 231 51 183 9.85e-34 123
MS.gene013182.t1 AT3G57800 46.763 139 24 2 144 235 201 336 5.90e-28 112
MS.gene013182.t1 AT2G24260 65.753 73 24 1 140 212 28 99 1.41e-23 97.1
MS.gene013182.t1 AT2G24260 65.753 73 24 1 140 212 131 202 1.17e-22 96.7
MS.gene013182.t1 AT5G58010 52.941 102 37 3 147 244 99 193 2.34e-22 95.1
MS.gene013182.t1 AT4G30980 71.212 66 18 1 147 212 130 194 3.32e-22 94.7
MS.gene013182.t1 AT4G30980 71.212 66 18 1 147 212 178 242 4.67e-22 95.1
MS.gene013182.t1 AT2G42300 83.721 43 7 0 144 186 182 224 6.18e-19 84.0
MS.gene013182.t1 AT2G42300 83.721 43 7 0 144 186 182 224 6.18e-19 84.0
MS.gene013182.t1 AT2G42300 83.721 43 7 0 144 186 182 224 7.63e-19 84.0
MS.gene013182.t1 AT4G09180 53.731 67 31 0 145 211 182 248 3.41e-15 74.3
MS.gene013182.t1 AT1G35460 53.846 65 30 0 147 211 181 245 3.48e-15 74.3
MS.gene013182.t1 AT2G42280 54.688 64 29 0 147 210 279 342 5.59e-15 75.1
MS.gene013182.t1 AT2G42280 56.061 66 27 1 147 210 279 344 8.45e-15 74.3
MS.gene013182.t1 AT1G51140 57.812 64 27 0 147 210 304 367 8.82e-15 74.3
MS.gene013182.t1 AT4G28790 47.297 74 38 1 149 222 273 345 1.71e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT1G09530 43.820 89 46 2 124 212 317 401 1.93e-14 73.9
MS.gene013182.t1 AT4G02590 40.310 129 63 4 109 224 36 163 4.54e-14 70.9
MS.gene013182.t1 AT4G28800 48.611 72 36 1 150 221 252 322 4.83e-14 72.4
MS.gene013182.t1 AT4G02590 40.310 129 63 4 109 224 99 226 5.06e-14 71.6
MS.gene013182.t1 AT4G02590 40.310 129 63 4 109 224 99 226 5.06e-14 71.6
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 210 271 6.65e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 210 271 6.65e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 210 271 6.65e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 281 342 6.95e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 281 342 6.95e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 281 342 6.95e-14 72.0
MS.gene013182.t1 AT2G20180 52.381 63 29 1 150 212 281 342 6.95e-14 72.0
MS.gene013182.t1 AT4G28815 50.000 70 33 2 150 218 71 139 7.34e-14 70.1
MS.gene013182.t1 AT4G28815 50.000 70 33 2 150 218 147 215 2.45e-13 69.7
MS.gene013182.t1 AT1G03040 53.659 82 33 2 147 224 139 219 3.96e-13 68.9
MS.gene013182.t1 AT1G03040 53.659 82 33 2 147 224 144 224 4.37e-13 68.9
MS.gene013182.t1 AT2G43140 53.125 64 30 0 147 210 134 197 6.08e-13 67.0
MS.gene013182.t1 AT2G43140 53.125 64 30 0 147 210 231 294 7.13e-13 68.2
MS.gene013182.t1 AT1G03040 53.659 82 33 2 147 224 144 224 7.76e-13 68.6
MS.gene013182.t1 AT4G28800 52.459 61 28 1 150 210 264 323 1.37e-12 68.2
MS.gene013182.t1 AT2G20180 51.667 60 28 1 150 209 281 339 1.86e-12 67.4
MS.gene013182.t1 AT1G27740 36.170 141 72 5 86 223 118 243 3.17e-12 65.9
MS.gene013182.t1 AT4G00050 49.275 69 34 1 143 211 101 168 1.26e-11 64.7
MS.gene013182.t1 AT4G00050 49.275 69 34 1 143 211 203 270 2.27e-11 64.3
MS.gene013182.t1 AT1G05805 48.438 64 33 0 147 210 283 346 2.28e-11 64.3
MS.gene013182.t1 AT4G00050 49.275 69 34 1 143 211 203 270 2.81e-11 63.9
MS.gene013182.t1 AT4G28790 45.455 66 35 1 149 214 273 337 4.54e-11 63.2
MS.gene013182.t1 AT2G14760 45.349 86 42 3 147 228 237 321 4.97e-11 63.2
MS.gene013182.t1 AT4G33880 50.769 65 31 1 147 211 266 329 9.71e-11 62.4

Find 63 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGGGTTAGTATTGTTTC+TGG 0.191984 3.3:-81212396 None:intergenic
TCCACAGTCTTCCATGGTTC+TGG 0.296713 3.3:+81212307 MS.gene013182:CDS
GCAAAAGTTGTGCCTTGTTT+AGG 0.309108 3.3:-81213652 None:intergenic
CTCAGTTATGGTAGTAACTT+TGG 0.320242 3.3:-81212252 None:intergenic
AGCTGCTAATGACTACCTTT+TGG 0.330118 3.3:+81213711 MS.gene013182:CDS
ATGGATTGTAACCAGGTAAC+TGG 0.332974 3.3:+81213235 MS.gene013182:intron
AAAAGATCAGAGAAAGTGTT+TGG 0.372452 3.3:+81212807 MS.gene013182:CDS
GGACGTGGATATAGCCAGTT+GGG 0.373564 3.3:-81212837 None:intergenic
AACTGTGAAGGTGGAGGAGG+AGG 0.387298 3.3:-81212182 None:intergenic
AACAGACCTAGACACTCTTT+TGG 0.397586 3.3:+81213466 MS.gene013182:CDS
GTTCTGGACAAGCTTGAAAA+TGG 0.416319 3.3:+81212323 MS.gene013182:CDS
TGAAACTGTGAAGGTGGAGG+AGG 0.419275 3.3:-81212185 None:intergenic
AGAAGGTTCATTCTCAGTTA+TGG 0.434337 3.3:-81212264 None:intergenic
TTATCAGAAATTAAATAACT+TGG 0.449559 3.3:+81213588 MS.gene013182:intron
TGGCCTGACCCCTTCTTGCT+CGG 0.454614 3.3:-81212858 None:intergenic
TAGCCACAGCCTTGCTGAAA+GGG 0.456249 3.3:+81212885 MS.gene013182:CDS
GACCCCTTCTTGCTCGGACG+TGG 0.465774 3.3:-81212852 None:intergenic
CGTCCGAGCAAGAAGGGGTC+AGG 0.472206 3.3:+81212855 MS.gene013182:CDS
GAAGATGTTGGTTGGAGTCA+TGG 0.483883 3.3:-81213686 None:intergenic
GAAGAGAGAAAATAAGTGAA+AGG 0.496748 3.3:+81213048 MS.gene013182:CDS
GACACAGATGATAATGTTGA+TGG 0.508396 3.3:-81213616 None:intergenic
ATTAGCAGCTTGGAAGATGT+TGG 0.513901 3.3:-81213698 None:intergenic
AATATTATTACATGAGAGTG+AGG 0.517794 3.3:-81212413 None:intergenic
TGACAGGATAATGCAGCAGA+AGG 0.520312 3.3:+81212730 MS.gene013182:intron
AAGTTGCAGCAACTTGTGCC+AGG 0.526665 3.3:+81213077 MS.gene013182:CDS
GGGTGAAACTGTGAAGGTGG+AGG 0.526667 3.3:-81212188 None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG 0.530497 3.3:+81212884 MS.gene013182:CDS
ACTTGTGCCAGGCTGTGATA+AGG 0.531492 3.3:+81213088 MS.gene013182:CDS
AAAGGTAGTCATTAGCAGCT+TGG 0.531755 3.3:-81213708 None:intergenic
CCATGGAAGACTGTGGACTA+AGG 0.533292 3.3:-81212301 None:intergenic
CCTTAGTCCACAGTCTTCCA+TGG 0.535388 3.3:+81212301 MS.gene013182:CDS
GCAGCTTGGAAGATGTTGGT+TGG 0.538644 3.3:-81213694 None:intergenic
TTAATAATATCTTGATGAAG+AGG 0.545994 3.3:-81212209 None:intergenic
AGCAAGGCTGTGGCTATCAG+TGG 0.554257 3.3:-81212878 None:intergenic
TATCCACGTCCGAGCAAGAA+GGG 0.556525 3.3:+81212849 MS.gene013182:CDS
TTGAAGCTGAAGTATCCAAA+AGG 0.556829 3.3:-81213726 None:intergenic
GTTCTCCTCTTCTTCTCCGT+AGG 0.559768 3.3:-81212374 None:intergenic
CGGACGTGGATATAGCCAGT+TGG 0.559837 3.3:-81212838 None:intergenic
ATAGTCCTACGGAGAAGAAG+AGG 0.562653 3.3:+81212369 MS.gene013182:CDS
AATGATGAGTCAACAAGGAA+AGG 0.563177 3.3:-81212134 None:intergenic
ATGAGTCAACAAGGAAAGGA+TGG 0.563812 3.3:-81212130 None:intergenic
ATACCCTTTCAGCAAGGCTG+TGG 0.567250 3.3:-81212888 None:intergenic
TGACCTACCTTATCACAGCC+TGG 0.577941 3.3:-81213095 None:intergenic
GTCTAACCAAAAGAGTGTCT+AGG 0.588703 3.3:-81213472 None:intergenic
TTCAAGCTTGTCCAGAACCA+TGG 0.592122 3.3:-81212318 None:intergenic
GTTGCCATGTCATAGAACAT+AGG 0.595363 3.3:-81213446 None:intergenic
ATATCCACGTCCGAGCAAGA+AGG 0.602822 3.3:+81212848 MS.gene013182:CDS
AGAGGGTGAAACTGTGAAGG+TGG 0.604355 3.3:-81212191 None:intergenic
GTCTTTACAGAATCAAGTAG+AGG 0.604540 3.3:+81213297 MS.gene013182:CDS
GTCATCAAATGTTGTCTCTG+AGG 0.606377 3.3:+81213768 MS.gene013182:CDS
TTGTAACCAGGTAACTGGAA+AGG 0.609989 3.3:+81213240 MS.gene013182:intron
ATATTATTACATGAGAGTGA+GGG 0.611838 3.3:-81212412 None:intergenic
TAATAATATCTTGATGAAGA+GGG 0.621927 3.3:-81212208 None:intergenic
GACGTGGATATAGCCAGTTG+GGG 0.624086 3.3:-81212836 None:intergenic
GACAGGATAATGCAGCAGAA+GGG 0.624145 3.3:+81212731 MS.gene013182:intron
ATCCACGTCCGAGCAAGAAG+GGG 0.635705 3.3:+81212850 MS.gene013182:CDS
TCCAGAACCATGGAAGACTG+TGG 0.648687 3.3:-81212308 None:intergenic
GCATGAATGATGAGTCAACA+AGG 0.650211 3.3:-81212139 None:intergenic
CATTGCAATAGTCCTAAACA+AGG 0.657273 3.3:+81213640 MS.gene013182:CDS
TGTACTATACCCTTTCAGCA+AGG 0.661128 3.3:-81212894 None:intergenic
TGAAGAGGGTGAAACTGTGA+AGG 0.663457 3.3:-81212194 None:intergenic
GAATCCTATGTTCTATGACA+TGG 0.666100 3.3:+81213442 MS.gene013182:CDS
ACGTGGATATAGCCAGTTGG+GGG 0.714907 3.3:-81212835 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTTACTTAATAATACT+TGG + chr3.3:81214042-81214061 MS.gene013182:intron 10.0%
!! AAAATGATTTAATCCTAAAA+TGG + chr3.3:81213813-81213832 MS.gene013182:intron 15.0%
!! AATTATATAATATAAGAGAC+AGG - chr3.3:81214023-81214042 None:intergenic 15.0%
!! TTATCAGAAATTAAATAACT+TGG + chr3.3:81213588-81213607 MS.gene013182:intron 15.0%
!!! GTGAATTTTAAATTAATGAA+AGG - chr3.3:81213951-81213970 None:intergenic 15.0%
!!! TTAGGATTAAATCATTTTTT+TGG - chr3.3:81213811-81213830 None:intergenic 15.0%
!! AAATTGGATGAATTATATTC+AGG + chr3.3:81213022-81213041 MS.gene013182:intron 20.0%
!! TAATAATATCTTGATGAAGA+GGG - chr3.3:81212211-81212230 None:intergenic 20.0%
!! TGTTAGTAATTAAATATGAC+AGG + chr3.3:81212714-81212733 MS.gene013182:intron 20.0%
!! TGTTGCAAATTGAATTAAAT+TGG + chr3.3:81213006-81213025 MS.gene013182:intron 20.0%
!! TTAATAATATCTTGATGAAG+AGG - chr3.3:81212212-81212231 None:intergenic 20.0%
!!! AAAAAATAACATTTTCAGGA+TGG + chr3.3:81213863-81213882 MS.gene013182:intron 20.0%
!!! AAACTTGTGGATAATTTTTT+TGG + chr3.3:81212575-81212594 MS.gene013182:intron 20.0%
!!! AGAATAATTACATCCATTTT+AGG - chr3.3:81213829-81213848 None:intergenic 20.0%
!!! CTCATAATTTTTTTTTCTTC+AGG + chr3.3:81214080-81214099 MS.gene013182:intron 20.0%
!!! TGACAAAAAATAACATTTTC+AGG + chr3.3:81213859-81213878 MS.gene013182:intron 20.0%
!!! TTAGCACTTTTTTTATACTA+TGG + chr3.3:81212608-81212627 MS.gene013182:intron 20.0%
! AATATTATTACATGAGAGTG+AGG - chr3.3:81212416-81212435 None:intergenic 25.0%
! AGACATGAAGATAACTAAAA+GGG - chr3.3:81212977-81212996 None:intergenic 25.0%
! ATATTATTACATGAGAGTGA+GGG - chr3.3:81212415-81212434 None:intergenic 25.0%
! TAATCAACTCAATTCATAAC+AGG - chr3.3:81212680-81212699 None:intergenic 25.0%
! TATATGATGATTCACATGTT+AGG + chr3.3:81213521-81213540 MS.gene013182:intron 25.0%
!! ATAACTAAAAGGGACATTTT+TGG - chr3.3:81212967-81212986 None:intergenic 25.0%
!! ATTATTTATGGATTGTAACC+AGG + chr3.3:81213228-81213247 MS.gene013182:intron 25.0%
!! CCACAAGTTTTCTTAAAATA+AGG - chr3.3:81212565-81212584 None:intergenic 25.0%
!!! CCTTATTTTAAGAAAACTTG+TGG + chr3.3:81212562-81212581 MS.gene013182:intron 25.0%
!!! TTTGAGACTTTTAATTGCAT+GGG - chr3.3:81213150-81213169 None:intergenic 25.0%
!!! TTTTGAGACTTTTAATTGCA+TGG - chr3.3:81213151-81213170 None:intergenic 25.0%
ATCTCTAATTCTTCTGTCAT+AGG - chr3.3:81212528-81212547 None:intergenic 30.0%
CAAAAGTTTCTTCATTCACA+TGG + chr3.3:81213905-81213924 MS.gene013182:intron 30.0%
GAAAAACAAAGAAGATGAGA+AGG + chr3.3:81212771-81212790 MS.gene013182:CDS 30.0%
GAAGAGAGAAAATAAGTGAA+AGG + chr3.3:81213048-81213067 MS.gene013182:CDS 30.0%
GAGACATGAAGATAACTAAA+AGG - chr3.3:81212978-81212997 None:intergenic 30.0%
TACTCAGAAAAATAGTCCTA+CGG + chr3.3:81212358-81212377 MS.gene013182:CDS 30.0%
TCTAGCAGTGATATATATGA+TGG - chr3.3:81212459-81212478 None:intergenic 30.0%
TTGGAATTGTATCATACAAG+TGG - chr3.3:81212948-81212967 None:intergenic 30.0%
! ACGAGTATTTTCATTTCATG+TGG + chr3.3:81212919-81212938 MS.gene013182:intron 30.0%
! AGTGATATATATGATGGAGA+TGG - chr3.3:81212453-81212472 None:intergenic 30.0%
! CAGCTTCAATTTTTCTACAA+GGG + chr3.3:81213740-81213759 MS.gene013182:CDS 30.0%
! CGAGTATTTTCATTTCATGT+GGG + chr3.3:81212920-81212939 MS.gene013182:intron 30.0%
! GAGTATTTTCATTTCATGTG+GGG + chr3.3:81212921-81212940 MS.gene013182:intron 30.0%
! TCAGCTTCAATTTTTCTACA+AGG + chr3.3:81213739-81213758 MS.gene013182:CDS 30.0%
!! AAAAGATCAGAGAAAGTGTT+TGG + chr3.3:81212807-81212826 MS.gene013182:CDS 30.0%
!! AATATTCTCAAGCTGTTTTG+TGG + chr3.3:81213327-81213346 MS.gene013182:intron 30.0%
!!! AAACATGTTGGTAGTTTTGT+AGG - chr3.3:81213986-81214005 None:intergenic 30.0%
!!! AACATGTTGGTAGTTTTGTA+GGG - chr3.3:81213985-81214004 None:intergenic 30.0%
AATGATGAGTCAACAAGGAA+AGG - chr3.3:81212137-81212156 None:intergenic 35.0%
AGAAGGTTCATTCTCAGTTA+TGG - chr3.3:81212267-81212286 None:intergenic 35.0%
CATTGCAATAGTCCTAAACA+AGG + chr3.3:81213640-81213659 MS.gene013182:CDS 35.0%
CTCAGTTATGGTAGTAACTT+TGG - chr3.3:81212255-81212274 None:intergenic 35.0%
GAATCCTATGTTCTATGACA+TGG + chr3.3:81213442-81213461 MS.gene013182:CDS 35.0%
GTCTTTACAGAATCAAGTAG+AGG + chr3.3:81213297-81213316 MS.gene013182:CDS 35.0%
TTGAGAGCTTACAAACATGT+TGG - chr3.3:81213998-81214017 None:intergenic 35.0%
! CAATTTTTCTACAAGGGCAA+AGG + chr3.3:81213746-81213765 MS.gene013182:CDS 35.0%
! GACACAGATGATAATGTTGA+TGG - chr3.3:81213619-81213638 None:intergenic 35.0%
! TTGAAGCTGAAGTATCCAAA+AGG - chr3.3:81213729-81213748 None:intergenic 35.0%
!! TTCAGGATGGTAGTCATTTT+TGG + chr3.3:81213876-81213895 MS.gene013182:intron 35.0%
!!! AGGTTTTTGGTGAAAGAAGA+AGG - chr3.3:81212284-81212303 None:intergenic 35.0%
!!! TCAGGATGGTAGTCATTTTT+GGG + chr3.3:81213877-81213896 MS.gene013182:intron 35.0%
AAAGGTAGTCATTAGCAGCT+TGG - chr3.3:81213711-81213730 None:intergenic 40.0%
ACCTTCATGACAGCACATAT+AGG + chr3.3:81213124-81213143 MS.gene013182:intron 40.0%
ATGAGTCAACAAGGAAAGGA+TGG - chr3.3:81212133-81212152 None:intergenic 40.0%
ATTAGCAGCTTGGAAGATGT+TGG - chr3.3:81213701-81213720 None:intergenic 40.0%
GCATGAATGATGAGTCAACA+AGG - chr3.3:81212142-81212161 None:intergenic 40.0%
GTCATCAAATGTTGTCTCTG+AGG + chr3.3:81213768-81213787 MS.gene013182:CDS 40.0%
GTTGCCATGTCATAGAACAT+AGG - chr3.3:81213449-81213468 None:intergenic 40.0%
TGTACTATACCCTTTCAGCA+AGG - chr3.3:81212897-81212916 None:intergenic 40.0%
TTGTAACCAGGTAACTGGAA+AGG + chr3.3:81213240-81213259 MS.gene013182:intron 40.0%
! AACAGACCTAGACACTCTTT+TGG + chr3.3:81213466-81213485 MS.gene013182:CDS 40.0%
! AGCTGCTAATGACTACCTTT+TGG + chr3.3:81213711-81213730 MS.gene013182:CDS 40.0%
! ATGGATTGTAACCAGGTAAC+TGG + chr3.3:81213235-81213254 MS.gene013182:intron 40.0%
! GTGAGGGTTAGTATTGTTTC+TGG - chr3.3:81212399-81212418 None:intergenic 40.0%
! GTTCTGGACAAGCTTGAAAA+TGG + chr3.3:81212323-81212342 MS.gene013182:CDS 40.0%
!! AGACTGTGGACTAAGGTTTT+TGG - chr3.3:81212297-81212316 None:intergenic 40.0%
!! CACTCTTTTGGTTAGACAAG+AGG + chr3.3:81213478-81213497 MS.gene013182:CDS 40.0%
!! GCAAAAGTTGTGCCTTGTTT+AGG - chr3.3:81213655-81213674 None:intergenic 40.0%
!! GTCTAACCAAAAGAGTGTCT+AGG - chr3.3:81213475-81213494 None:intergenic 40.0%
!!! GATGGTAGTCATTTTTGGGA+GGG + chr3.3:81213881-81213900 MS.gene013182:intron 40.0%
ATAGTCCTACGGAGAAGAAG+AGG + chr3.3:81212369-81212388 MS.gene013182:CDS 45.0%
GACAGGATAATGCAGCAGAA+GGG + chr3.3:81212731-81212750 MS.gene013182:intron 45.0%
GCCTATATGTGCTGTCATGA+AGG - chr3.3:81213128-81213147 None:intergenic 45.0%
TAAGAGCCTTTCCAGTTACC+TGG - chr3.3:81213249-81213268 None:intergenic 45.0%
TGAAGAGGGTGAAACTGTGA+AGG - chr3.3:81212197-81212216 None:intergenic 45.0%
TGACAGGATAATGCAGCAGA+AGG + chr3.3:81212730-81212749 MS.gene013182:intron 45.0%
TTCAAGCTTGTCCAGAACCA+TGG - chr3.3:81212321-81212340 None:intergenic 45.0%
! GAAGATGTTGGTTGGAGTCA+TGG - chr3.3:81213689-81213708 None:intergenic 45.0%
!!! GGATGGTAGTCATTTTTGGG+AGG + chr3.3:81213880-81213899 MS.gene013182:intron 45.0%
!! TCATAAAATATAATTATTTA+TGG + chr3.3:81213216-81213235 MS.gene013182:intron 5.0%
AAGTTGCAGCAACTTGTGCC+AGG + chr3.3:81213077-81213096 MS.gene013182:CDS 50.0%
ACGTGGATATAGCCAGTTGG+GGG - chr3.3:81212838-81212857 None:intergenic 50.0%
ACTTGTGCCAGGCTGTGATA+AGG + chr3.3:81213088-81213107 MS.gene013182:CDS 50.0%
AGAGGGTGAAACTGTGAAGG+TGG - chr3.3:81212194-81212213 None:intergenic 50.0%
ATACCCTTTCAGCAAGGCTG+TGG - chr3.3:81212891-81212910 None:intergenic 50.0%
ATAGCCACAGCCTTGCTGAA+AGG + chr3.3:81212884-81212903 MS.gene013182:CDS 50.0%
ATATCCACGTCCGAGCAAGA+AGG + chr3.3:81212848-81212867 MS.gene013182:CDS 50.0%
CATGTGTGTGTGTGTGTGCA+TGG - chr3.3:81212648-81212667 None:intergenic 50.0%
CCTTAGTCCACAGTCTTCCA+TGG + chr3.3:81212301-81212320 MS.gene013182:CDS 50.0%
GACGTGGATATAGCCAGTTG+GGG - chr3.3:81212839-81212858 None:intergenic 50.0%
GCAGCTTGGAAGATGTTGGT+TGG - chr3.3:81213697-81213716 None:intergenic 50.0%
GGACGTGGATATAGCCAGTT+GGG - chr3.3:81212840-81212859 None:intergenic 50.0%
GTTCTCCTCTTCTTCTCCGT+AGG - chr3.3:81212377-81212396 None:intergenic 50.0%
TAGCCACAGCCTTGCTGAAA+GGG + chr3.3:81212885-81212904 MS.gene013182:CDS 50.0%
TATCCACGTCCGAGCAAGAA+GGG + chr3.3:81212849-81212868 MS.gene013182:CDS 50.0%
TCCACAGTCTTCCATGGTTC+TGG + chr3.3:81212307-81212326 MS.gene013182:CDS 50.0%
TCCAGAACCATGGAAGACTG+TGG - chr3.3:81212311-81212330 None:intergenic 50.0%
TGAAACTGTGAAGGTGGAGG+AGG - chr3.3:81212188-81212207 None:intergenic 50.0%
TGACCTACCTTATCACAGCC+TGG - chr3.3:81213098-81213117 None:intergenic 50.0%
! CCATGGAAGACTGTGGACTA+AGG - chr3.3:81212304-81212323 None:intergenic 50.0%
AACTGTGAAGGTGGAGGAGG+AGG - chr3.3:81212185-81212204 None:intergenic 55.0%
AGCAAGGCTGTGGCTATCAG+TGG - chr3.3:81212881-81212900 None:intergenic 55.0%
ATCCACGTCCGAGCAAGAAG+GGG + chr3.3:81212850-81212869 MS.gene013182:CDS 55.0%
CGGACGTGGATATAGCCAGT+TGG - chr3.3:81212841-81212860 None:intergenic 55.0%
GGGTGAAACTGTGAAGGTGG+AGG - chr3.3:81212191-81212210 None:intergenic 55.0%
GTGCCAGGCTGTGATAAGGT+AGG + chr3.3:81213092-81213111 MS.gene013182:intron 55.0%
TGTTTGGATGAGCCCCCAAC+TGG + chr3.3:81212823-81212842 MS.gene013182:CDS 55.0%
TGGCCTGACCCCTTCTTGCT+CGG - chr3.3:81212861-81212880 None:intergenic 60.0%
CGTCCGAGCAAGAAGGGGTC+AGG + chr3.3:81212855-81212874 MS.gene013182:CDS 65.0%
GACCCCTTCTTGCTCGGACG+TGG - chr3.3:81212855-81212874 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 81212107 81214110 81212107 ID=MS.gene013182
chr3.3 mRNA 81212107 81214110 81212107 ID=MS.gene013182.t1;Parent=MS.gene013182
chr3.3 exon 81212107 81212424 81212107 ID=MS.gene013182.t1.exon1;Parent=MS.gene013182.t1
chr3.3 CDS 81212107 81212424 81212107 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81212736 81212906 81212736 ID=MS.gene013182.t1.exon2;Parent=MS.gene013182.t1
chr3.3 CDS 81212736 81212906 81212736 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81213044 81213109 81213044 ID=MS.gene013182.t1.exon3;Parent=MS.gene013182.t1
chr3.3 CDS 81213044 81213109 81213044 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81213250 81213318 81213250 ID=MS.gene013182.t1.exon4;Parent=MS.gene013182.t1
chr3.3 CDS 81213250 81213318 81213250 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81213416 81213499 81213416 ID=MS.gene013182.t1.exon5;Parent=MS.gene013182.t1
chr3.3 CDS 81213416 81213499 81213416 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81213595 81213789 81213595 ID=MS.gene013182.t1.exon6;Parent=MS.gene013182.t1
chr3.3 CDS 81213595 81213789 81213595 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
chr3.3 exon 81214102 81214110 81214102 ID=MS.gene013182.t1.exon7;Parent=MS.gene013182.t1
chr3.3 CDS 81214102 81214110 81214102 ID=cds.MS.gene013182.t1;Parent=MS.gene013182.t1
Gene Sequence

>MS.gene013182

ATGGCAGCTTTTTCATATCAATACCATCCTTTCCTTGTTGACTCATCATTCATGCTCAACATGAACACTGCTTCTCCTCCTCCTCCACCTTCACAGTTTCACCCTCTTCATCAAGATATTATTAACTGTGTTGATCAACAAAGCTCCAAAGTTACTACCATAACTGAGAATGAACCTTCTTCTTTCACCAAAAACCTTAGTCCACAGTCTTCCATGGTTCTGGACAAGCTTGAAAATGGTGATGAGCAAGTTACTCAGAAAAATAGTCCTACGGAGAAGAAGAGGAGAACCAGAAACAATACTAACCCTCACTCTCATGTAATAATATTAATTGTTTCTGTTTCCATCTCCATCATATATATCACTGCTAGAAAAAACTAAAATTATCAATCAAAATTATAGACAAAAATATAAAATTCCTATGACAGAAGAATTAGAGATATGTTTAATGTTTTCCTTATTTTAAGAAAACTTGTGGATAATTTTTTTGGTAATGTATTTTTAGCACTTTTTTTATACTATGGTTTCATTAATTAAGCCATGCACACACACACACACATGTGTTTTGTTCCTGTTATGAATTGAGTTGATTATTTTGTACTGAATTTGTTAGTAATTAAATATGACAGGATAATGCAGCAGAAGGGAAAAACAAGAAACTGAAGAAAAACAAAGAAGATGAGAAGGCAAAAGCAGAGAAAAAAGATCAGAGAAAGTGTTTGGATGAGCCCCCAACTGGCTATATCCACGTCCGAGCAAGAAGGGGTCAGGCCACTGATAGCCACAGCCTTGCTGAAAGGGTATAGTACATTACGAGTATTTTCATTTCATGTGGGGTCCACTTGTATGATACAATTCCAAAAATGTCCCTTTTAGTTATCTTCATGTCTCATTCTGAATGTTGCAAATTGAATTAAATTGGATGAATTATATTCAGGTAAGAAGAGAGAAAATAAGTGAAAGGATGAAGAAGTTGCAGCAACTTGTGCCAGGCTGTGATAAGGTAGGTCAAGTAAAACCTTCATGACAGCACATATAGGCCCATGCAATTAAAAGTCTCAAAACTATACTGTTGAAAATAATATCTTTAATAATGAACATTCTATTTTTCATAAAATATAATTATTTATGGATTGTAACCAGGTAACTGGAAAGGCTCTTATGTTAGATGAAATAATCAATTATGTTCAGTCTTTACAGAATCAAGTAGAGGTAACATCAATATTCTCAAGCTGTTTTGTGGTTATTTATTTACTTAAAATGTATAAATATACTAATTAACAAGCTATATGCTCTCTATTTTTTTCAGTTCTTGTCAATGAAACTTACTTCTGTGAATCCTATGTTCTATGACATGGCAACAGACCTAGACACTCTTTTGGTTAGACAAGAGGTGAGTAAGAAAATTCAGTGATATATGATGATTCACATGTTAGGTCTAATAATGAAATTAACTAACTAACTTAGTATTTTTGAATGAATTATCAGAAATTAAATAACTTGGCATCACCATCAACATTATCATCTGTGTCACATTGCAATAGTCCTAAACAAGGCACAACTTTTGCTGATACAACAACCATGACTCCAACCAACATCTTCCAAGCTGCTAATGACTACCTTTTGGATACTTCAGCTTCAATTTTTCTACAAGGGCAAAGGTCATCAAATGTTGTCTCTGAGGTATAAAAACACAAATAACCAAAAAAATGATTTAATCCTAAAATGGATGTAATTATTCTTTTCTTATTCTGACAAAAAATAACATTTTCAGGATGGTAGTCATTTTTGGGAGGGACAAAAGTTTCTTCATTCACATGGATTCAACAGCAACTTATGTTCCTTTCATTAATTTAAAATTCACAGATGTGCATGCCCTACAAAACTACCAACATGTTTGTAAGCTCTCAAATCCTGTCTCTTATATTATATAATTTAATTTACTTAATAATACTTGGTTTCTAATTCTAATACTCATAATTTTTTTTTCTTCAGGATTTTTAG
Protein sequence

>MS.gene013182.t1

MAAFSYQYHPFLVDSSFMLNMNTASPPPPPSQFHPLHQDIINCVDQQSSKVTTITENEPSSFTKNLSPQSSMVLDKLENGDEQVTQKNSPTEKKRRTRNNTNPHSHDNAAEGKNKKLKKNKEDEKAKAEKKDQRKCLDEPPTGYIHVRARRGQATDSHSLAERVRREKISERMKKLQQLVPGCDKVTGKALMLDEIINYVQSLQNQVEFLSMKLTSVNPMFYDMATDLDTLLVRQEKLNNLASPSTLSSVSHCNSPKQGTTFADTTTMTPTNIFQAANDYLLDTSASIFLQGQRSSNVVSEDF