AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01342

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01342.t1 MTR_2g011940 97.757 758 13 2 1 758 135 888 0.0 1518
MS.gene01342.t1 MTR_2g011940 96.443 506 15 1 1 506 144 646 0.0 1011
MS.gene01342.t1 MTR_2g011940 95.127 513 22 1 1 513 144 653 0.0 1009
MS.gene01342.t1 MTR_4g028130 54.167 552 219 10 168 704 270 802 0.0 563
MS.gene01342.t1 MTR_4g028130 51.531 588 215 13 168 704 270 838 0.0 551
MS.gene01342.t1 MTR_3g467380 48.759 564 229 11 157 678 283 828 5.90e-165 500
MS.gene01342.t1 MTR_4g028130 56.131 367 124 4 168 497 270 636 1.56e-130 402
MS.gene01342.t1 MTR_5g075410 46.801 297 118 4 174 431 283 578 2.12e-77 265
MS.gene01342.t1 MTR_5g075410 41.040 173 82 5 510 678 618 774 5.67e-25 111
MS.gene01342.t1 MTR_7g073240 44.481 308 130 6 180 484 89 358 3.43e-76 250
MS.gene01342.t1 MTR_7g083700 39.394 132 71 3 212 339 126 252 1.47e-19 90.9
MS.gene01342.t1 MTR_7g059330 38.235 102 61 2 212 311 623 724 2.05e-15 80.9
MS.gene01342.t1 MTR_1g058350 35.484 124 72 4 195 312 58 179 2.47e-14 75.1
MS.gene01342.t1 MTR_8g023990 38.182 110 60 4 212 315 171 278 3.17e-14 76.6
MS.gene01342.t1 MTR_7g117455 38.679 106 57 4 212 311 117 220 1.89e-13 73.9
MS.gene01342.t1 MTR_7g105370 41.000 100 52 5 217 312 65 161 6.66e-13 70.5
MS.gene01342.t1 MTR_1g093600 33.094 139 73 5 212 332 191 327 3.50e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01342.t1 AT4G32010 52.990 485 218 6 168 644 237 719 3.28e-161 486
MS.gene01342.t1 AT4G32010 52.556 489 218 7 168 644 237 723 6.74e-159 480
MS.gene01342.t1 AT2G30470 46.441 562 269 8 111 645 182 738 9.11e-147 449
MS.gene01342.t1 AT4G21550 40.506 395 189 10 44 429 169 526 2.52e-73 249
MS.gene01342.t1 AT4G21550 40.476 420 200 11 43 453 168 546 4.54e-70 244
MS.gene01342.t1 AT4G21550 44.720 161 60 4 525 685 531 662 8.65e-22 101
MS.gene01342.t1 AT4G21550 39.895 381 183 10 44 415 169 512 1.48e-67 233
MS.gene01342.t1 AT3G26790 41.346 104 60 1 212 314 91 194 2.12e-19 90.5
MS.gene01342.t1 AT3G24650 35.172 145 76 4 175 311 538 672 2.68e-16 83.6
MS.gene01342.t1 AT2G36080 39.815 108 56 4 212 312 37 142 5.47e-15 75.9
MS.gene01342.t1 AT2G36080 39.815 108 56 4 212 312 37 142 5.76e-15 75.5
MS.gene01342.t1 AT5G06250 37.391 115 56 4 212 312 45 157 6.06e-15 76.3
MS.gene01342.t1 AT2G36080 39.815 108 56 4 212 312 37 142 6.17e-15 73.9
MS.gene01342.t1 AT2G36080 39.815 108 56 4 212 312 37 142 6.17e-15 73.9
MS.gene01342.t1 AT5G06250 37.391 115 56 4 212 312 45 157 6.22e-15 76.6
MS.gene01342.t1 AT5G06250 37.391 115 56 4 212 312 45 157 6.89e-15 76.3
MS.gene01342.t1 AT5G06250 37.391 115 56 4 212 312 45 157 8.91e-15 75.9
MS.gene01342.t1 AT5G06250 37.391 115 56 4 212 312 45 157 1.04e-14 75.5
MS.gene01342.t1 AT1G28300 39.000 100 59 2 198 296 157 255 4.53e-14 75.1
MS.gene01342.t1 AT3G11580 36.207 116 57 4 212 312 28 141 1.07e-13 72.4
MS.gene01342.t1 AT4G01500 40.449 89 50 2 212 299 35 121 2.08e-13 72.8
MS.gene01342.t1 AT3G11580 36.207 116 57 4 212 312 28 141 7.12e-13 69.3
MS.gene01342.t1 AT3G11580 36.207 116 57 4 212 312 28 141 7.12e-13 69.3
MS.gene01342.t1 AT3G11580 36.207 116 57 4 212 312 28 141 7.12e-13 69.3
MS.gene01342.t1 AT2G46870 39.560 91 50 3 212 299 34 122 8.72e-13 70.5
MS.gene01342.t1 AT3G11580 36.207 116 57 4 212 312 28 141 3.67e-12 69.3
MS.gene01342.t1 AT3G25730 39.362 94 49 4 212 299 182 273 3.70e-12 68.9
MS.gene01342.t1 AT1G25560 34.746 118 63 5 212 317 194 309 5.39e-12 68.6

Find 207 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCACTCTCGTACCTTTC+TGG 0.160003 2.2:+70068063 None:intergenic
AATGAAAGAAATGGTCAAAT+TGG 0.226571 2.2:-70072949 MS.gene01342:CDS
CTTTCATTTGGGGATGAATA+AGG 0.231314 2.2:+70072964 None:intergenic
TTGTCGCCTGGTTCAGATTC+TGG 0.241849 2.2:-70067049 MS.gene01342:CDS
ATTTGACCATTTCTTTCATT+TGG 0.245941 2.2:+70072952 None:intergenic
TTTGACCATTTCTTTCATTT+GGG 0.271314 2.2:+70072953 None:intergenic
TGTCTGGAATATCAGCTAAT+TGG 0.273177 2.2:-70073246 MS.gene01342:CDS
GGATATTTCAGTTCCGCTTC+TGG 0.286219 2.2:-70071364 MS.gene01342:CDS
TACATTCTGCTGTTGTTATT+CGG 0.300641 2.2:+70071341 None:intergenic
GCTGGATATTGAGATCAATT+TGG 0.303683 2.2:+70067089 None:intergenic
AACCCTTCAGGCTGAGAAAT+TGG 0.305767 2.2:+70071422 None:intergenic
TTGCCCTCCTTGTTCATAGT+TGG 0.309184 2.2:+70067169 None:intergenic
AATGGTCAAATTGGGGTAAC+TGG 0.309222 2.2:-70072940 MS.gene01342:CDS
TCATCCGTGTCTCCGTTTAA+AGG 0.315648 2.2:-70067112 MS.gene01342:CDS
GCCAGTGATGCTGGGCGAAT+TGG 0.316030 2.2:-70072560 MS.gene01342:CDS
CAGACTGGGTGCTTGCTTCC+AGG 0.319580 2.2:+70073399 None:intergenic
GCCTGAAGGGAGGTTGGTTA+TGG 0.323362 2.2:-70070390 MS.gene01342:CDS
ATCTATCGCTTAGGTTGAAT+TGG 0.326369 2.2:-70069615 MS.gene01342:intron
AATAACTTTGACAAAATATA+TGG 0.331049 2.2:-70073316 MS.gene01342:CDS
AGCTAATTGGAATGTCAAAC+TGG 0.337122 2.2:-70073233 MS.gene01342:CDS
GTGCACTTCTTCCAGCAAAA+TGG 0.337367 2.2:-70068110 MS.gene01342:CDS
TGCCCTCCTTGTTCATAGTT+GGG 0.342106 2.2:+70067170 None:intergenic
ATGAAAGAAATGGTCAAATT+GGG 0.345350 2.2:-70072948 MS.gene01342:CDS
GGCCCTTTCCACTAGGCGGC+TGG 0.351565 2.2:+70067404 None:intergenic
CCAGTGATGCTGGGCGAATT+GGG 0.353628 2.2:-70072559 MS.gene01342:CDS
GTGCATGTCTGCTTATGCTT+GGG 0.356470 2.2:+70067382 None:intergenic
TCCTGATCATGCTGAAGCTT+TGG 0.362667 2.2:-70067545 MS.gene01342:CDS
CCTGAAGGGAGGTTGGTTAT+GGG 0.369927 2.2:-70070389 MS.gene01342:CDS
TTTCAGATGAAGGCAGTTGC+TGG 0.379421 2.2:+70067242 None:intergenic
CTTAGCAACGGTGTTATTCA+TGG 0.385576 2.2:-70066890 MS.gene01342:CDS
TCTTCTCACGCCGTAACATA+AGG 0.386896 2.2:+70067320 None:intergenic
AATTGGCTGATCCTAGTTCC+TGG 0.387306 2.2:-70069598 MS.gene01342:CDS
CCCATAACCAACCTCCCTTC+AGG 0.389700 2.2:+70070389 None:intergenic
GTGGGTGTTTAGTTGTGGCC+TGG 0.391891 2.2:+70067461 None:intergenic
TTACAGGATGCTCCGGTGCT+TGG 0.393729 2.2:-70068718 MS.gene01342:intron
GTTGGTTATGGGATTTAGAA+AGG 0.394057 2.2:-70070378 MS.gene01342:CDS
CCCAATTCGCCCAGCATCAC+TGG 0.395558 2.2:+70072559 None:intergenic
GTCAGTTGTTGCCACGGTAT+TGG 0.401190 2.2:-70072789 MS.gene01342:CDS
ACAATGCTTGGGACATGATT+AGG 0.404239 2.2:+70069395 None:intergenic
TACAGGATGCTCCGGTGCTT+GGG 0.413480 2.2:-70068717 MS.gene01342:intron
GGACATGTTCAGATAATTCA+TGG 0.414352 2.2:-70068089 MS.gene01342:CDS
TTGCTTATGAACAGTAATAC+AGG 0.417703 2.2:-70073196 MS.gene01342:intron
TTGGCCCAGGTGTACTGAAT+TGG 0.418132 2.2:-70072770 MS.gene01342:CDS
GATTCATCTTCTCAACCGTT+TGG 0.422726 2.2:-70072997 MS.gene01342:CDS
CGCAGTAACATTTAGCCGGT+TGG 0.424036 2.2:-70070414 MS.gene01342:intron
AGATCAATTTGGCCTTTAAA+CGG 0.428590 2.2:+70067100 None:intergenic
GACAATTCTTCTTCTCGCTC+TGG 0.431144 2.2:+70067067 None:intergenic
ACTTGCAAATCTAGCAATCC+TGG 0.433506 2.2:-70067509 MS.gene01342:CDS
CCTTCAATCACCACAATGCT+TGG 0.436508 2.2:+70069383 None:intergenic
CTTTGAGTTTGAGGAATATG+AGG 0.436772 2.2:-70069360 MS.gene01342:intron
ACTTTGAGTGCCAGTGATGC+TGG 0.442164 2.2:-70072569 MS.gene01342:CDS
CATCTAGGCAAGCAGTTTAG+TGG 0.442863 2.2:-70072892 MS.gene01342:CDS
GGTGCTTGCTTCCAGGGTAC+AGG 0.444210 2.2:+70073406 None:intergenic
ACACGTTCATAGACAAAGAC+TGG 0.448302 2.2:+70066834 None:intergenic
TCCTTGCATAATAGTAATAC+AGG 0.448760 2.2:-70067202 MS.gene01342:CDS
CATTTACCTCTTGACTCATC+AGG 0.448985 2.2:-70072862 MS.gene01342:CDS
TGTGCATGTCTGCTTATGCT+TGG 0.449862 2.2:+70067381 None:intergenic
AAATCCTTTGCCATCCGATC+AGG 0.452416 2.2:-70070351 MS.gene01342:intron
TGGGTGTTTAGTTGTGGCCT+GGG 0.452762 2.2:+70067462 None:intergenic
CAGTAGTCATAATGCTGATA+AGG 0.453305 2.2:-70066864 MS.gene01342:CDS
TGCTGGGCGAATTGGGCGCT+TGG 0.455987 2.2:-70072552 MS.gene01342:CDS
TCAACTAGAGAATGCACATC+TGG 0.456237 2.2:+70073346 None:intergenic
TCATCCCCAAATGAAAGAAA+TGG 0.456820 2.2:-70072958 MS.gene01342:CDS
TGATCATGCTGAAGCTTTGG+AGG 0.457477 2.2:-70067542 MS.gene01342:CDS
TCAACATTTGACCAGGAACT+AGG 0.459867 2.2:+70069587 None:intergenic
CTTAAAATCCTCGATGCAAA+AGG 0.461243 2.2:-70071395 MS.gene01342:CDS
CAACAAACTCCACCTCATCT+AGG 0.464917 2.2:-70072907 MS.gene01342:CDS
TGGTCAAATGTTGACAAGTC+AGG 0.467081 2.2:-70069578 MS.gene01342:CDS
GGAATGTCAAACTGGGTTCA+AGG 0.467906 2.2:-70073225 MS.gene01342:CDS
ATTCTTCTTCTCGCTCTGGC+TGG 0.467907 2.2:+70067071 None:intergenic
TTAGTTGTGGCCTGGGAAGA+TGG 0.468284 2.2:+70067469 None:intergenic
GCAAACAATGCAAGAGCAGC+CGG 0.469885 2.2:+70067429 None:intergenic
GCAACAACTGACTTCTTCCT+CGG 0.470752 2.2:+70072800 None:intergenic
TGACTCATCAGGTGAAGCTC+AGG 0.473085 2.2:-70072851 MS.gene01342:CDS
TGGAGTCAGTTAGCTGGATC+AGG 0.473225 2.2:-70073430 MS.gene01342:CDS
CCAAGCATTGTGGTGATTGA+AGG 0.474053 2.2:-70069383 MS.gene01342:CDS
ATGAGGAATGAGGACAAATC+AGG 0.474622 2.2:-70073063 MS.gene01342:CDS
ACCTCCCTTCAGGCTCCAAC+CGG 0.476212 2.2:+70070399 None:intergenic
TTATCACTAGTGAAAATAGA+TGG 0.477763 2.2:+70068691 None:intergenic
CCACCAATTTCTCAGCCTGA+AGG 0.479735 2.2:-70071425 MS.gene01342:CDS
TCCAAAGCTTCAGCATGATC+AGG 0.479989 2.2:+70067544 None:intergenic
ATCCAGCCGCCTAGTGGAAA+GGG 0.481833 2.2:-70067406 MS.gene01342:CDS
GATAATGAAGCTAATAAGAC+TGG 0.483266 2.2:-70069733 MS.gene01342:CDS
GAATCTTCCTTTAGAGTATG+AGG 0.483887 2.2:+70073015 None:intergenic
CCTTTAGAGTATGAGGCCGT+TGG 0.485973 2.2:+70073022 None:intergenic
CAATTCAAACCTGAATTACA+AGG 0.486493 2.2:+70067721 None:intergenic
GTTTCCGTACCCTTATGTTA+CGG 0.488608 2.2:-70067330 MS.gene01342:CDS
CTGGATCAGGACCTGTACCC+TGG 0.491579 2.2:-70073417 MS.gene01342:CDS
GAAGCTAATAAGACTGGAAA+CGG 0.491637 2.2:-70069727 MS.gene01342:CDS
TCTGGTACTGGAGATTCATC+AGG 0.492385 2.2:-70066956 MS.gene01342:CDS
TCAAGCAAAATTTCAGATGA+AGG 0.493848 2.2:+70067232 None:intergenic
GTCACCTGCTTGCAACTGCA+TGG 0.495052 2.2:+70071286 None:intergenic
GAAAATGAAGGCCACTAAGC+AGG 0.495097 2.2:-70067569 MS.gene01342:CDS
GATTGAAGGCTTTGAGTTTG+AGG 0.495120 2.2:-70069369 MS.gene01342:CDS
TGTTGCCACGGTATTGGCCC+AGG 0.495577 2.2:-70072783 MS.gene01342:CDS
TAGCTCCAATTCAGTACACC+TGG 0.498477 2.2:+70072765 None:intergenic
CTTCTCACGCCGTAACATAA+GGG 0.500342 2.2:+70067321 None:intergenic
ATGATCAGGATCCTGCTTAG+TGG 0.500883 2.2:+70067558 None:intergenic
AGACTGGGTGCTTGCTTCCA+GGG 0.501160 2.2:+70073400 None:intergenic
GGAGGCTCTCCCATCTTCCC+AGG 0.502386 2.2:-70067479 MS.gene01342:CDS
CTGAGAAATTGGTGGGAAGT+AGG 0.504512 2.2:+70071433 None:intergenic
GAGCAGCCGGGTCTATGTCG+TGG 0.505284 2.2:+70067442 None:intergenic
ATGAACAAGGAGGGCAATGA+TGG 0.506662 2.2:-70067163 MS.gene01342:CDS
ACAAAATATATGGCAATGAA+AGG 0.507284 2.2:-70073306 MS.gene01342:CDS
TTCAGATGAAGGCAGTTGCT+GGG 0.508534 2.2:+70067243 None:intergenic
CATCCAGCCGCCTAGTGGAA+AGG 0.510624 2.2:-70067407 MS.gene01342:CDS
GAGACATGTCTGGCAAAAGT+TGG 0.514952 2.2:-70073450 None:intergenic
TTTGTTGTATTGCAGGAATG+AGG 0.515892 2.2:-70073080 MS.gene01342:intron
ATCAGGTAGTCAGTCACAGC+AGG 0.516556 2.2:-70066939 MS.gene01342:CDS
CCAACGGCCTCATACTCTAA+AGG 0.527271 2.2:-70073022 MS.gene01342:CDS
ACTTTGTTGAACTCTGGTAC+TGG 0.527710 2.2:-70066968 MS.gene01342:CDS
ATACATAGCAAAAGAAGCAC+TGG 0.528619 2.2:-70069555 MS.gene01342:CDS
CAGCAGACTTTGTTGAACTC+TGG 0.530003 2.2:-70066974 MS.gene01342:CDS
CTTGCAAATCTAGCAATCCT+GGG 0.532774 2.2:-70067508 MS.gene01342:CDS
CTTCAATCACCACAATGCTT+GGG 0.534367 2.2:+70069384 None:intergenic
TGCTTGGGGCCCTTTCCACT+AGG 0.534401 2.2:+70067397 None:intergenic
AGATGAGGTGGAGTTTGTTG+TGG 0.534680 2.2:+70072910 None:intergenic
GAGAGATATAGAGACATGTC+TGG 0.539014 2.2:-70073460 None:intergenic
CAACAACTGACTTCTTCCTC+GGG 0.544517 2.2:+70072801 None:intergenic
GTGAAGCTCAGGTTCGCAAT+GGG 0.546771 2.2:-70072840 MS.gene01342:CDS
CAGTCCATGCAGTTGCAAGC+AGG 0.549929 2.2:-70071290 MS.gene01342:CDS
GAATGTCAAACTGGGTTCAA+GGG 0.552101 2.2:-70073224 MS.gene01342:CDS
GAGAATGCACATCTGGAGGC+TGG 0.553030 2.2:+70073353 None:intergenic
TAGTTGTGGCCTGGGAAGAT+GGG 0.553270 2.2:+70067470 None:intergenic
TGCATGTCTGCTTATGCTTG+GGG 0.556813 2.2:+70067383 None:intergenic
CGAGAAGAAGAATTGTCGCC+TGG 0.557693 2.2:-70067061 MS.gene01342:CDS
ATAATTCATGGGATCCAGAA+AGG 0.562018 2.2:-70068077 MS.gene01342:intron
GTTGGAGCCTGAAGGGAGGT+TGG 0.564406 2.2:-70070396 MS.gene01342:CDS
ACCTGTATTACTATTATGCA+AGG 0.565040 2.2:+70067201 None:intergenic
AAAAGTTGGAGTCAGTTAGC+TGG 0.568196 2.2:-70073436 MS.gene01342:CDS
AGAAGCTGAATTGAACAGAC+TGG 0.568636 2.2:+70073384 None:intergenic
AACTGCTTGCCTAGATGAGG+TGG 0.570136 2.2:+70072898 None:intergenic
TGCTTGGGACATGATTAGGA+GGG 0.573196 2.2:+70069399 None:intergenic
TAATAGAGCTGAAGATTACA+TGG 0.574564 2.2:-70069448 MS.gene01342:CDS
CACCAATTTCTCAGCCTGAA+GGG 0.575416 2.2:-70071424 MS.gene01342:CDS
AGCAGCCGGGTCTATGTCGT+GGG 0.575738 2.2:+70067443 None:intergenic
TTTAGCCGGTTGGAGCCTGA+AGG 0.576874 2.2:-70070404 MS.gene01342:CDS
CCTTCAGGCTGAGAAATTGG+TGG 0.578628 2.2:+70071425 None:intergenic
ATAGATGGCTTCCCAAGCAC+CGG 0.579291 2.2:+70068706 None:intergenic
CAATGCAGATATTACCTGAT+CGG 0.581192 2.2:+70070337 None:intergenic
GCAGATATTACCTGATCGGA+TGG 0.582173 2.2:+70070341 None:intergenic
ATGTCGTGGGTGTTTAGTTG+TGG 0.582924 2.2:+70067456 None:intergenic
GGTGAAGCTCAGGTTCGCAA+TGG 0.584201 2.2:-70072841 MS.gene01342:CDS
TAAGGTACAGATAAACCAAA+CGG 0.588759 2.2:+70072982 None:intergenic
GATCATGCTGAAGCTTTGGA+GGG 0.590184 2.2:-70067541 MS.gene01342:CDS
GCAGGTAGGAGATGTTGTGA+GGG 0.592609 2.2:-70066921 MS.gene01342:CDS
AATCCTCGATGCAAAAGGAA+AGG 0.594386 2.2:-70071390 MS.gene01342:CDS
TACATAGCAAAAGAAGCACT+GGG 0.595326 2.2:-70069554 MS.gene01342:CDS
AGCAGTTTCCAAGAAAATGA+AGG 0.595741 2.2:-70067581 MS.gene01342:CDS
TTGACCATTTCTTTCATTTG+GGG 0.596631 2.2:+70072954 None:intergenic
GCTGGAAGAAGTGCACATGC+TGG 0.600458 2.2:+70068117 None:intergenic
CAGTTGCAAGCAGGTGACAC+AGG 0.601495 2.2:-70071281 MS.gene01342:intron
GTTTGCATCCAGCCGCCTAG+TGG 0.603172 2.2:-70067412 MS.gene01342:CDS
CAAACAATGCAAGAGCAGCC+GGG 0.603823 2.2:+70067430 None:intergenic
CTTCCTCGGGCATCTGCTCG+AGG 0.605073 2.2:+70072814 None:intergenic
ACACCACCAGAATCTGAACC+AGG 0.607711 2.2:+70067043 None:intergenic
ATTACCTGATCGGATGGCAA+AGG 0.607719 2.2:+70070347 None:intergenic
ATGCACATCTGGAGGCTGGT+CGG 0.608646 2.2:+70073357 None:intergenic
TGTTGTTATTCGGCCAGAAG+CGG 0.609669 2.2:+70071351 None:intergenic
CGTGTTTAAATATGTGCCAA+CGG 0.609774 2.2:-70073038 MS.gene01342:CDS
GACATGTTCAGATAATTCAT+GGG 0.609897 2.2:-70068088 MS.gene01342:CDS
GCCGGTTGGAGCCTGAAGGG+AGG 0.610013 2.2:-70070400 MS.gene01342:CDS
TGACTTGTCAACATTTGACC+AGG 0.610368 2.2:+70069580 None:intergenic
TTAGCCGGTTGGAGCCTGAA+GGG 0.610617 2.2:-70070403 MS.gene01342:CDS
ATGCTTGGGACATGATTAGG+AGG 0.611515 2.2:+70069398 None:intergenic
CTTCAGGCTGAGAAATTGGT+GGG 0.611961 2.2:+70071426 None:intergenic
CACGTTCATAGACAAAGACT+GGG 0.613315 2.2:+70066835 None:intergenic
TGAAAGAAATGGTCAAATTG+GGG 0.613362 2.2:-70072947 MS.gene01342:CDS
CTTTGAGTGCCAGTGATGCT+GGG 0.613991 2.2:-70072568 MS.gene01342:CDS
GAAGAAGTCAGTTGTTGCCA+CGG 0.618200 2.2:-70072795 MS.gene01342:CDS
CTAAACTGCTTGCCTAGATG+AGG 0.618575 2.2:+70072895 None:intergenic
AATTCAAACCTGAATTACAA+GGG 0.620703 2.2:+70067722 None:intergenic
TCGATGCAAAAGGAAAGGAA+TGG 0.623875 2.2:-70071385 MS.gene01342:CDS
ATTACATGGCAAGAAGCTCA+AGG 0.624749 2.2:-70069434 MS.gene01342:CDS
AGCAGGTAGGAGATGTTGTG+AGG 0.624795 2.2:-70066922 MS.gene01342:CDS
CTTGGGACATGATTAGGAGG+GGG 0.628705 2.2:+70069401 None:intergenic
TTGGCCTTTAAACGGAGACA+CGG 0.628717 2.2:+70067108 None:intergenic
AGCTCCAATTCAGTACACCT+GGG 0.629096 2.2:+70072766 None:intergenic
CGTTGGCACATATTTAAACA+CGG 0.631143 2.2:+70073039 None:intergenic
CGACCTCGAGCAGATGCCCG+AGG 0.632234 2.2:-70072817 MS.gene01342:CDS
GGTAGTCAGTCACAGCAGGT+AGG 0.633855 2.2:-70066935 MS.gene01342:CDS
GCTTCACCTGATGAGTCAAG+AGG 0.634530 2.2:+70072856 None:intergenic
GCTAATTGGAATGTCAAACT+GGG 0.636082 2.2:-70073232 MS.gene01342:CDS
TATTGCAGGAATGAGGAATG+AGG 0.637424 2.2:-70073073 MS.gene01342:intron
AGCCCAACTATGAACAAGGA+GGG 0.637935 2.2:-70067172 MS.gene01342:CDS
TTGCAAATCTAGCAATCCTG+GGG 0.642083 2.2:-70067507 MS.gene01342:CDS
ATTCAAACCTGAATTACAAG+GGG 0.643071 2.2:+70067723 None:intergenic
TAATCATGTCCCAAGCATTG+TGG 0.643328 2.2:-70069393 MS.gene01342:CDS
AATGTGAAGATTGTCTGAAG+TGG 0.644577 2.2:-70068149 MS.gene01342:CDS
GTTCTGATGATGATCCTCAC+CGG 0.646876 2.2:-70067141 MS.gene01342:CDS
GCTTGGGACATGATTAGGAG+GGG 0.648870 2.2:+70069400 None:intergenic
ACTAGAGAATGCACATCTGG+AGG 0.649096 2.2:+70073349 None:intergenic
CACGCCGTAACATAAGGGTA+CGG 0.656740 2.2:+70067326 None:intergenic
TAGTAGCCCAACTATGAACA+AGG 0.657573 2.2:-70067176 MS.gene01342:CDS
GAAGCTGAATTGAACAGACT+GGG 0.665746 2.2:+70073385 None:intergenic
AAACACCCACGACATAGACC+CGG 0.669434 2.2:-70067448 MS.gene01342:CDS
AACTGCATGGACTGTATGCA+CGG 0.670080 2.2:+70071299 None:intergenic
AGGGAAGTGAAGCTTAGCAA+CGG 0.671793 2.2:-70066902 MS.gene01342:CDS
CACATGTAATGTCTGTCTAA+CGG 0.673914 2.2:-70067362 MS.gene01342:CDS
TTGGGGCCCTTTCCACTAGG+CGG 0.682669 2.2:+70067400 None:intergenic
TCGCCTGGTTCAGATTCTGG+TGG 0.694439 2.2:-70067046 MS.gene01342:CDS
ACATCTCAGCAGCAGCATCG+TGG 0.696358 2.2:+70067008 None:intergenic
GTACACCTGGGCCAATACCG+TGG 0.704903 2.2:+70072778 None:intergenic
TAGCCCAACTATGAACAAGG+AGG 0.716754 2.2:-70067173 MS.gene01342:CDS
TGCAAATCTAGCAATCCTGG+GGG 0.721871 2.2:-70067506 MS.gene01342:CDS
TTCAAACCTGAATTACAAGG+GGG 0.743313 2.2:+70067724 None:intergenic
ACATAGCAAAAGAAGCACTG+GGG 0.799937 2.2:-70069553 MS.gene01342:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.2 gene 70066832 70073467 70066832 ID=MS.gene01342
chr2.2 mRNA 70066832 70073467 70066832 ID=MS.gene01342.t1;Parent=MS.gene01342
chr2.2 exon 70073197 70073467 70073197 ID=MS.gene01342.t1.exon1;Parent=MS.gene01342.t1
chr2.2 CDS 70073197 70073467 70073197 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70072748 70073087 70072748 ID=MS.gene01342.t1.exon2;Parent=MS.gene01342.t1
chr2.2 CDS 70072748 70073087 70072748 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70072529 70072625 70072529 ID=MS.gene01342.t1.exon3;Parent=MS.gene01342.t1
chr2.2 CDS 70072529 70072625 70072529 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70071282 70071456 70071282 ID=MS.gene01342.t1.exon4;Parent=MS.gene01342.t1
chr2.2 CDS 70071282 70071456 70071282 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70070352 70070431 70070352 ID=MS.gene01342.t1.exon5;Parent=MS.gene01342.t1
chr2.2 CDS 70070352 70070431 70070352 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70069711 70069755 70069711 ID=MS.gene01342.t1.exon6;Parent=MS.gene01342.t1
chr2.2 CDS 70069711 70069755 70069711 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70069361 70069624 70069361 ID=MS.gene01342.t1.exon7;Parent=MS.gene01342.t1
chr2.2 CDS 70069361 70069624 70069361 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70068685 70068734 70068685 ID=MS.gene01342.t1.exon8;Parent=MS.gene01342.t1
chr2.2 CDS 70068685 70068734 70068685 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70068078 70068191 70068078 ID=MS.gene01342.t1.exon9;Parent=MS.gene01342.t1
chr2.2 CDS 70068078 70068191 70068078 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70067731 70067801 70067731 ID=MS.gene01342.t1.exon10;Parent=MS.gene01342.t1
chr2.2 CDS 70067731 70067801 70067731 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
chr2.2 exon 70066832 70067601 70066832 ID=MS.gene01342.t1.exon11;Parent=MS.gene01342.t1
chr2.2 CDS 70066832 70067601 70066832 ID=cds.MS.gene01342.t1;Parent=MS.gene01342.t1
Gene Sequence

>MS.gene01342

ATGTCTGGCAAAAGTTGGAGTCAGTTAGCTGGATCAGGACCTGTACCCTGGAAGCAAGCACCCAGTCTGTTCAATTCAGCTTCTTCATCCGACCAGCCTCCAGATGTGCATTCTCTAGTTGATTTATCAAATAACTTTGACAAAATATATGGCAATGAAAGGTTGCCCCCCTCATCATTGGAAAAGAAAAATGAGGATTTGTCTGGAATATCAGCTAATTGGAATGTCAAACTGGGTTCAAGGGACATGTTGCTTATGAACAGTAATACAGGTTTTGACCTTCTCAACTGTTTACTTTTTCAGTTACAGGTTTAGGAGGTAGATGAATCTTTTTATAACATGGAGATGGCTACATAACCGGATTCTTTGTTGTATTGCAGGAATGAGGAATGAGGACAAATCAGGTCCGTGTTTAAATATGTGCCAACGGCCTCATACTCTAAAGGAAGATTCATCTTCTCAACCGTTTGGTTTATCTGTACCTTATTCATCCCCAAATGAAAGAAATGGTCAAATTGGGGTAACTGGAATTCATCCACAACAAACTCCACCTCATCTAGGCAAGCAGTTTAGTGGCACTATGCATTTACCTCTTGACTCATCAGGTGAAGCTCAGGTTCGCAATGGGCGACCTCGAGCAGATGCCCGAGGAAGAAGTCAGTTGTTGCCACGGTATTGGCCCAGGTGTACTGAATTGGAGCTACAGCAAATAGCTGTCGAGTATCCTTCAAATTACATACTTATTAAGTAATTACATTGGTTTACATATATTTTAATGTACTTAATTTTTCAATTATGTATAAATCCATATCGGAATGTACAATATCTTAACTTCACCCTAGTTCTAATTCTGTAATAACTCCCTTGTTCCAAAAAACTTTGAGTGCCAGTGATGCTGGGCGAATTGGGCGCTTGGTACTACCCAAAAAATGTGCTGAGGTTGGTCTTGAGATCCTCTCTGCTAGCTATTAATTTGAAAATATTTTTGGATCGAGTATGTGTTGTGTGAATGCTTCATGCCCATAGTAAATTGAATTTAAAGTGATTAACAATTGATTGTCTGCCTTTTGTAATGCCTGGCCTGCATGTCATAAATCTCTTGCTTTTTGCCCTGTAACTTTGGTACACTGATAAGCTCCTTGTTTGGCATGTAAATTAGTGTATACTGCACAATGTGGTGTTAGTTTTTGTGGCAAGTTCTTATGGCATGACAATATTATTCTTCTATCATTTGTACTTCTCTAGCAAGTCTTTCAATAGATTGTAGAAACCAGGAAGGGTAAAACTGCATTAATAAAATCAGGAAGATTGTCTTCCCTGGAGCAACAGAGAGTAGAAAGTAGAAACCAAGAAAACATCAGCATGAGAGTATATGTATTAGCCAATTCCCACCTAAATGGTCTATCTTTAGCTTAATGTATGGTAGAGGATTGCTGCATCATTTAATCCATGTAGCCAACTCCACCTAGTGAGATAAGGCTTGGTGGTGATTGTTACCGTATGATTTATGACCAAATAGTCTTCCTAGGTTATTTATCATCTCTGTTAATCTGTCTTTGGGGATTCCTTATACTGATATGATCTTGGAAGTCTTTGTTGAGCTAATTACTTTATCATTGTATTTTTGTGCATTGAAGGACATGTGTGTTGTTCTCTGTTTCTGTTCCCCCCAAAAAAATTATGCAACAAAATTTAGTAGTATCGAAAGAAGATTGCAATATGCCCACCAAAGTTCATATGCTTTAAGAAAATGGAAAGTGTTATTCTTCAGAAGCATGTTAAAACTGTATCTCATGAACTATACTGATAATATGACTGTTTCCTTGAAATATAGTGTGATGCAATCATTACACACTCGTATCATTTTGGATTGCTCTGTGGATTTGTGGGATTACTTCTGGGCTTTAGCAATAGTCTTTTAATGTTTTGCATGGCACAGCCACCTTACTTTTTCATGATATTTAATAAAAAGTTATGCTATAATAATATTATATTTTGTTCCATGAGACAGACCTACTTCCCACCAATTTCTCAGCCTGAAGGGTTACCTCTTAAAATCCTCGATGCAAAAGGAAAGGAATGGATATTTCAGTTCCGCTTCTGGCCGAATAACAACAGCAGAATGTATGTTTTAGAGGGTGTAACTCCGTGCATACAGTCCATGCAGTTGCAAGCAGGTGACACAGGTAAGCCAATGGCATGAAGGAAATTTATGTGTTTGATTATTTAGAGTATTCTTATTTCCTTCCTTCTGAGTAAAACTTAAGCTTAATGGGTCAGATATGCACGGACCTTCCTTCTGGCCCTCGTTTAGAATTGTAGGTATAGGGTTGGAATTAAGAAAAACAATCGCCCTGAATATGTTATTGGGGTGTAATATGCTAATAATTTGTTCACCTGCTCTAGAAGCATATCTGTGCATTTGTTAGACCTGTGTTTCTTGTGTCTTTAAAAAAGGACAACGAACAGGCCCTTTGTAAGGAACGAAATAATCAACATATTCTTTGATAATCTTAAGATATTCTTGGCATCTCCCTACATACTTTGGAGTTGTTCAAAATCTGAACTTGATTCTATTGTTTTCCATCCGAAAGTTTTCTGTTTTCTAAACTTTCTTCAGTGATAACTTGCCTGATAACACTAGGTGTTGGAATTTCCGCCTTCATTGCGGTCAGCCCCTAACCGCCTAATTGCTGGCATTTAGGTTGCCATCATTGCAGCCTCCAGCACTACGTAATGACCTATGTGGTGCTTATAAAGCTTGGGCAGCCGTCACCTTACAAGCCAGTTTTGTAAGGTTGAGTTAGGCCCAACCCAAGTTCTAATACTAGGCATATATTGATGTTTGTTACAGCGCTTCTAGGTCATTATGCTCTGAAATATTGCCTCAAATAATCCCTGTTGAATCCTTGTCTTGGGTGCTTTTATGTCTTCTCCCCTCCCATTGTATCCTGTGTTGTACCACAACCTTGTTGTGGTTGATTAGTTGTGAGTTAATTATCTTGTATTGTGGCTGACCTGAACTGATTTTCGCAGTAACATTTAGCCGGTTGGAGCCTGAAGGGAGGTTGGTTATGGGATTTAGAAAGGCTACAAATCCTTTGCCATCCGATCAGGTAATATCTGCATTGTCTAATTGTCTGGGCTGTGTACATAATTTGAGTGTACATTGTATGGTTGAATGGAACTAACTTCAACTGGAACTTGTTTGGATAGTTTATGCTTGGAATTGGTTAGAAACGTGTACTAAATTGTATGGTTGAGTGTAGATTGTATGGTTGGATGGAACTAGCTTTAACTGGAATTTATGTGGATAGTTTATGCATGAAATTGGTTAGAAACATGTACTGAATCGGAATTTGCTTTGATATAGATTTTTTTGGATATGGTGTGACTGTTATGCCCTTTTGTAATTTCTGATTTCAATTTTTATATGCTGTTTGGATTCAGCTCAGGTTAATATGGCTAATCTTATAGTGTTGGATATTTCCTTTCTATTTTTTTTTGACAAAAGTTGTGGATATTTCCTAATTACTATAACGGATAGTTCATGAATTTATTCAAACTTTTCTTATTTAGTGCATATTTTATTTATTTTTTTTTGGTTACACGTATTTAATCTAATACATCCTATATCTATTTGTATAAAGTTCGGTGAAATTTTAATGTCTACTAAGAAAGTACATCCGTTCACGTATTTTTCTTTTTGCAGGATAATGAAGCTAATAAGACTGGAAACGGATTTTCTGCTCACGAAGTTAGTATAAAATAACTAACTGTATTGTAATTGCTAATTTGTCATTTAAGTCTCATACCTGGTTGCATTTTTCATCTATCGCTTAGGTTGAATTGGCTGATCCTAGTTCCTGGTCAAATGTTGACAAGTCAGGATACATAGCAAAAGAAGCACTGGGGAGCAAATCTTTAATCTCTAGAAAGAGAAAAAACAACATATTGGGTTCAAAAAGTAAACGTCTAAAAATTGAAAATGAGGATATAATAGAGCTGAAGATTACATGGCAAGAAGCTCAAGGCCTGCTTCGTCCCCCTCCTAATCATGTCCCAAGCATTGTGGTGATTGAAGGCTTTGAGTTTGAGGAATATGAGGTACCATTTCTCTTGCTTTGTTTCAGAAATGGTTTTGACATGCTAATGTAATTTAACTTTTAGTGATGTGTCAATCTAAACCTAAATGTGCTTTCTTTAAGTATACCAATAATATAAATTAATACGCTTTCATCTTCGGATAGTAATTCTTTGAATCCTTTGTTTATATAAAACTAGCCAAATGATAATTAGAACGTGTCCATGATGCAGGAGATATAGTTCTTCCATTTTGCGTTCTTAGTTGAAATGTAGTCAATCGTCCATGTTGTTATAAGACTTGTAAACAGAATTGTAAGTTCCTAAGTCACAGACATAACAAACAGGGAATTAAAGTGATGAAGGAGAGTTGCTTTGTGCATTCAATCATCACAAATGATCTTCAACTGGTTTTAGTTGAGGATTGTGTATTATAGACACTCATATTCCCAAACAATCCTTCGTCCTCGCCATCTTGTAGCCTGTCTGTGAACATAGATACTATTTTGTTGAAGTCACTTGGCTCGTCTTATATTGGATTGAAATTATTGTGTCTGTGTTGTAATCTTTCTTGGTATTTTATTTGATTGAAATTTTTATGAGAATTGCTATGCATTGTGGAAACCTTTATGCTGAGAATGCTTTTACAGGATGCTCCGGTGCTTGGGAAGCCATCTATTTTCACTAGTGATAATATGGGGTAAATAACTCATCCTTAATTCGGTTTTGATCAAATTGTATTGGTCAATTTTAAATTTTGCACATCTGATTTTTTGTATTATTTTTATATGGTGTGGAATTATGTTTTGTATGTGTGACATGCCTGTTGACTATTGTTGTTGAGTTATCATTAATTGATTATAATAACGACCATGATGTTTTGGATTTGATTGTGTTGTTGCTCTCACCTGGAGACTAGCATTGACTTTTGAGTGTTTATGAAGGAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTGCTGTGCATGTTTATCAGGTAGTTTCTAAGAAAGACATGATTTATACGCACACATACATATATAATTTAATTAAGTTCTGATTTAGACTGGGGGATCTGTTTATTGTAATTAACTAAGTAATTAATTGCTTGCAATGTACTTTATTTTCCAGTGAAAGGATTCAGTATACTCAATGTGAAGATTGTCTGAAGTGGCGCAAATTGCCAGCATGTGCACTTCTTCCAGCAAAATGGACATGTTCAGATAATTCATGGGATCCAGAAAGGTACGAGAGTGATCCATTCACTTGTTAACACATCCTTCCACATGTAAAACAGATTGGTTGGGAAAAAATATAAGAAATGGAAGTAGTGGTCGAAAAATTGACAATGTACCATACCATGCTTCATGTCAGTCAGGATACATTGTAATTTCTTGCACTTTTCCAGTCTTCCTCAACTTATTCCATGCTTATTTGCACAAAGCACATCATGAACCTATATAAATATGCAATCAATACACTAGTGTTTTATTTTTATTTGCAAATCCTCTTTGCTGCCAGATCTTCTTGTTCAGCTGCACAAGAGCTTACAACAGAACAACTTGAGAATTTGTTACCCCCTTGTAATTCAGGTTTGAATTGTTTTATTTCTTCACTTGAACAAATGTCTCAGATTCCTTGTCATGGTGTATGTAATATATAATATAATAAATTGCTAAACAAGGGTATGGAAACTATTGTTAATTGCATTTGTTTGGCAGCAGTTTCCAAGAAAATGAAGGCCACTAAGCAGGATCCTGATCATGCTGAAGCTTTGGAGGGACTTGATACACTTGCAAATCTAGCAATCCTGGGGGAAGGGGAGGCTCTCCCATCTTCCCAGGCCACAACTAAACACCCACGACATAGACCCGGCTGCTCTTGCATTGTTTGCATCCAGCCGCCTAGTGGAAAGGGCCCCAAGCATAAGCAGACATGCACATGTAATGTCTGTCTAACGGTGAAGCGTCGTTTCCGTACCCTTATGTTACGGCGTGAGAAGAAACAGTCAGAAAAAGAAGCAGAAACAACACGTAAAAAGCAGCAGCAACAGAATTCCCAGCAACTGCCTTCATCTGAAATTTTGCTTGACGATGATTCCTTGCATAATAGTAATACAGGTGATAGTAGCCCAACTATGAACAAGGAGGGCAATGATGGTTCTGATGATGATCCTCACCGGATAAAATCATCCGTGTCTCCGTTTAAAGGCCAAATTGATCTCAATATCCAGCCAGAGCGAGAAGAAGAATTGTCGCCTGGTTCAGATTCTGGTGGTGTGATGAAGCTGCTCCACGATGCTGCTGCTGAGATGTATCTCAAGCAGCAGCAGACTTTGTTGAACTCTGGTACTGGAGATTCATCAGGTAGTCAGTCACAGCAGGTAGGAGATGTTGTGAGGGAAGTGAAGCTTAGCAACGGTGTTATTCATGGTAGCAGTAGTCATAATGCTGATAAGGAACATGCCCAGTCTTTGTCTATGAACGTGTGA
Protein sequence

>MS.gene01342.t1

MSGKSWSQLAGSGPVPWKQAPSLFNSASSSDQPPDVHSLVDLSNNFDKIYGNERLPPSSLEKKNEDLSGISANWNVKLGSRDMLLMNSNTGMRNEDKSGPCLNMCQRPHTLKEDSSSQPFGLSVPYSSPNERNGQIGVTGIHPQQTPPHLGKQFSGTMHLPLDSSGEAQVRNGRPRADARGRSQLLPRYWPRCTELELQQIAVDSNSVITPLFQKTLSASDAGRIGRLVLPKKCAETYFPPISQPEGLPLKILDAKGKEWIFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRLEPEGRLVMGFRKATNPLPSDQDNEANKTGNGFSAHEVELADPSSWSNVDKSGYIAKEALGSKSLISRKRKNNILGSKSKRLKIENEDIIELKITWQEAQGLLRPPPNHVPSIVVIEGFEFEEYEDAPVLGKPSIFTSDNMGERIQYTQCEDCLKWRKLPACALLPAKWTCSDNSWDPERSSCSAAQELTTEQLENLLPPCNSAVSKKMKATKQDPDHAEALEGLDTLANLAILGEGEALPSSQATTKHPRHRPGCSCIVCIQPPSGKGPKHKQTCTCNVCLTVKRRFRTLMLRREKKQSEKEAETTRKKQQQQNSQQLPSSEILLDDDSLHNSNTGDSSPTMNKEGNDGSDDDPHRIKSSVSPFKGQIDLNIQPEREEELSPGSDSGGVMKLLHDAAAEMYLKQQQTLLNSGTGDSSGSQSQQVGDVVREVKLSNGVIHGSSSHNADKEHAQSLSMNV