AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013437


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013437.t1 MTR_3g093860 92.357 157 9 2 1 155 1 156 2.73e-103 292
MS.gene013437.t1 MTR_1g049100 60.448 134 52 1 14 146 4 137 6.02e-55 171
MS.gene013437.t1 MTR_3g078613 60.769 130 50 1 19 148 24 152 9.77e-55 171
MS.gene013437.t1 MTR_3g078613 61.069 131 49 2 19 148 24 153 1.79e-53 168
MS.gene013437.t1 MTR_5g036480 60.606 132 47 2 19 146 17 147 4.98e-52 165
MS.gene013437.t1 MTR_8g038620 57.353 136 51 1 19 147 10 145 1.11e-51 162
MS.gene013437.t1 MTR_4g106590 60.305 131 50 2 19 148 27 156 1.64e-51 163
MS.gene013437.t1 MTR_7g490310 59.091 132 46 2 19 142 10 141 1.52e-50 162
MS.gene013437.t1 MTR_3g088630 53.191 141 48 2 19 142 23 162 6.96e-46 150
MS.gene013437.t1 MTR_3g015490 52.143 140 50 3 19 147 5 138 5.93e-43 140
MS.gene013437.t1 MTR_6g007460 40.659 182 54 3 19 147 12 192 6.62e-37 126
MS.gene013437.t1 MTR_2g050020 91.379 58 4 1 1 58 35 91 9.71e-31 107
MS.gene013437.t1 MTR_4g051330 56.863 51 22 0 97 147 77 127 3.71e-16 71.2
MS.gene013437.t1 MTR_4g061360 34.959 123 70 1 20 142 91 203 8.56e-16 73.9
MS.gene013437.t1 MTR_1g067110 34.959 123 70 1 20 142 87 199 6.41e-15 71.2
MS.gene013437.t1 MTR_1g067110 34.959 123 70 1 20 142 87 199 7.80e-15 70.9
MS.gene013437.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.26e-14 68.2
MS.gene013437.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.27e-14 68.2
MS.gene013437.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.41e-14 68.2
MS.gene013437.t1 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.70e-14 68.2
MS.gene013437.t1 MTR_8g024260 30.894 123 75 1 20 142 38 150 3.14e-13 66.2
MS.gene013437.t1 MTR_7g118260 30.894 123 75 1 20 142 16 128 4.29e-13 65.9
MS.gene013437.t1 MTR_7g118260 30.894 123 75 1 20 142 16 128 5.02e-13 65.9
MS.gene013437.t1 MTR_8g079940 28.906 128 78 2 20 147 31 145 9.94e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013437.t1 AT3G56380 72.308 130 36 0 19 148 21 150 2.34e-64 194
MS.gene013437.t1 AT3G56380 72.519 131 35 1 19 148 21 151 4.69e-63 191
MS.gene013437.t1 AT2G40670 70.896 134 33 2 19 148 30 161 7.07e-63 191
MS.gene013437.t1 AT2G40670 71.111 135 32 3 19 148 30 162 1.45e-61 187
MS.gene013437.t1 AT1G10470 61.719 128 48 1 19 146 21 147 3.30e-53 169
MS.gene013437.t1 AT1G59940 58.647 133 54 1 14 146 29 160 4.81e-53 168
MS.gene013437.t1 AT1G10470 61.719 128 48 1 19 146 35 161 6.13e-53 168
MS.gene013437.t1 AT1G59940 58.647 133 54 1 14 146 121 252 7.17e-52 167
MS.gene013437.t1 AT3G48100 57.364 129 55 0 19 147 26 154 1.29e-51 162
MS.gene013437.t1 AT5G62920 58.955 134 54 1 19 152 26 158 3.10e-51 162
MS.gene013437.t1 AT3G57040 53.623 138 55 1 19 147 10 147 6.22e-51 162
MS.gene013437.t1 AT3G57040 53.623 138 55 1 19 147 10 147 6.22e-51 162
MS.gene013437.t1 AT2G41310 51.724 145 63 2 10 147 1 145 2.24e-50 161
MS.gene013437.t1 AT1G74890 59.091 132 53 1 19 150 19 149 5.77e-50 159
MS.gene013437.t1 AT1G19050 58.015 131 52 2 19 148 25 153 3.33e-49 157
MS.gene013437.t1 AT1G10470 50.000 74 33 3 75 146 14 85 3.10e-15 69.7
MS.gene013437.t1 AT5G24470 31.452 124 73 2 20 142 52 164 6.86e-14 68.2
MS.gene013437.t1 AT5G02810 31.707 123 74 1 20 142 80 192 2.07e-12 64.3
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.24e-12 63.9
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 2.25e-12 63.9
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene013437.t1 AT5G60100 33.333 123 72 1 20 142 66 178 5.14e-12 62.8
MS.gene013437.t1 AT2G46790 28.226 124 79 1 19 142 38 151 1.85e-11 61.2
MS.gene013437.t1 AT2G46790 28.226 124 79 1 19 142 38 151 2.23e-11 61.2
MS.gene013437.t1 AT5G07210 30.081 123 72 3 20 142 18 126 3.21e-11 60.8

Find 39 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGAAGGCATTGGAGTATT+TGG 0.158019 3.4:-85602434 MS.gene013437:CDS
GACAGGCCAGCTGATGAATT+TGG 0.201243 3.4:-85601810 MS.gene013437:CDS
ACAGTGACAACTGCTGAAAA+TGG 0.248549 3.4:-85602457 MS.gene013437:intron
CTGAAGGCATTGGAGTATTT+GGG 0.249714 3.4:-85602433 MS.gene013437:CDS
GTAGGGACCCAAAGTGAATT+TGG 0.331645 3.4:-85602237 MS.gene013437:intron
AATTACTTGTTGATCCTTGT+TGG 0.359191 3.4:+85601986 None:intergenic
TCAAAGTGGGTGATGGAAAA+TGG 0.361841 3.4:-85602727 MS.gene013437:CDS
TGAGTTGCTTAAGAAAATTA+AGG 0.374516 3.4:-85602177 MS.gene013437:intron
ACAGGCCAGCTGATGAATTT+GGG 0.393986 3.4:-85601809 MS.gene013437:CDS
TACTGCATGCCAGGGATGAC+TGG 0.411671 3.4:-85602203 MS.gene013437:CDS
GTGATGACCAAATTCACTTT+GGG 0.424646 3.4:+85602230 None:intergenic
ATCACAGACTACTGCATGCC+AGG 0.428078 3.4:-85602212 MS.gene013437:CDS
TTCTTCTTCAAAGTGGGTGA+TGG 0.456607 3.4:-85602734 MS.gene013437:CDS
CTGATTCTTCTTCTTCAAAG+TGG 0.467692 3.4:-85602741 MS.gene013437:CDS
TGATTCTTCTTCTTCAAAGT+GGG 0.471982 3.4:-85602740 MS.gene013437:CDS
ACCTTCCCAAATTCATCAGC+TGG 0.476038 3.4:+85601804 None:intergenic
TCAGATGACATGATCACAAC+AGG 0.480037 3.4:+85602016 None:intergenic
TCAGCAATCATCTGTGATGA+AGG 0.481432 3.4:-85602044 MS.gene013437:intron
TGTGATGACCAAATTCACTT+TGG 0.486577 3.4:+85602229 None:intergenic
GAACAGAACACTTTGGAGAA+TGG 0.490659 3.4:-85602397 MS.gene013437:CDS
CAGTGACAACTGCTGAAAAT+GGG 0.502968 3.4:-85602456 MS.gene013437:CDS
TTTACATCAGATTGTTTGAG+AGG 0.513388 3.4:+85601839 None:intergenic
TGAAAATGGGCTGAAGGCAT+TGG 0.515465 3.4:-85602443 MS.gene013437:CDS
AACTCATAGCCAGTCATCCC+TGG 0.516855 3.4:+85602194 None:intergenic
AAAAGCAGGTGTATGGAAGA+AGG 0.535716 3.4:-85601884 MS.gene013437:intron
AAAGCAGGTGTATGGAAGAA+GGG 0.542181 3.4:-85601883 MS.gene013437:intron
AACTGCTGAAAATGGGCTGA+AGG 0.568007 3.4:-85602449 MS.gene013437:CDS
CTCAAGAATTCCTCTTGCAA+AGG 0.589496 3.4:-85602640 MS.gene013437:intron
GCCAGCTGATGAATTTGGGA+AGG 0.598518 3.4:-85601805 MS.gene013437:CDS
AAGCAGGTGTATGGAAGAAG+GGG 0.612241 3.4:-85601882 MS.gene013437:intron
TCACAGACTACTGCATGCCA+GGG 0.612620 3.4:-85602211 MS.gene013437:CDS
CTTTGCAAGAGGAATTCTTG+AGG 0.621330 3.4:+85602641 None:intergenic
TCTGATGTAAAACAACTGAC+AGG 0.621647 3.4:-85601827 MS.gene013437:CDS
TCATCAACAGCAAGAACATG+AGG 0.625979 3.4:+85602697 None:intergenic
AAGTGGTGAACAGAACACTT+TGG 0.626039 3.4:-85602404 MS.gene013437:CDS
GAGTATTTGGGCTTGTCAAG+TGG 0.627865 3.4:-85602421 MS.gene013437:CDS
GGTGTACGTACCTTTGCAAG+AGG 0.636893 3.4:+85602630 None:intergenic
GCAATCATCTGTGATGAAGG+AGG 0.667691 3.4:-85602041 MS.gene013437:CDS
CATCTGAGAATATACCAACA+AGG 0.683899 3.4:-85602000 MS.gene013437:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATATACAAAAAAAA+TGG + chr3.4:85602601-85602620 None:intergenic 10.0%
!! AAAAAGAATATAACAGAAAA+TGG + chr3.4:85601948-85601967 None:intergenic 15.0%
!! CTGAAAATAATATCCAATTT+GGG + chr3.4:85602478-85602497 None:intergenic 20.0%
!! TGAAAATAATATCCAATTTG+GGG + chr3.4:85602477-85602496 None:intergenic 20.0%
!!! TGATGTTTGATGTTTTTTTT+TGG - chr3.4:85602006-85602025 MS.gene013437:CDS 20.0%
!!! TTTTTTTTTGGTCCATATTT+TGG - chr3.4:85602018-85602037 MS.gene013437:CDS 20.0%
! GCTGAAAATAATATCCAATT+TGG + chr3.4:85602479-85602498 None:intergenic 25.0%
! TGAGTTGCTTAAGAAAATTA+AGG - chr3.4:85602361-85602380 MS.gene013437:intron 25.0%
!!! GTGTTTTGTTAGTAACATTT+TGG + chr3.4:85602199-85602218 None:intergenic 25.0%
AAATAATATCCAATTTGGGG+TGG + chr3.4:85602474-85602493 None:intergenic 30.0%
CAAACAGAATCACCAAAATA+TGG + chr3.4:85602033-85602052 None:intergenic 30.0%
GTGATTCTGTTTGATTGAAT+GGG - chr3.4:85602040-85602059 MS.gene013437:CDS 30.0%
TGATTCTTCTTCTTCAAAGT+GGG - chr3.4:85601798-85601817 MS.gene013437:CDS 30.0%
TTTACATCAGATTGTTTGAG+AGG + chr3.4:85602702-85602721 None:intergenic 30.0%
! AATTACTTGTTGATCCTTGT+TGG + chr3.4:85602555-85602574 None:intergenic 30.0%
!!! GACTATTTCTGGTTTTTGTA+GGG - chr3.4:85602284-85602303 MS.gene013437:intron 30.0%
!!! TGACTATTTCTGGTTTTTGT+AGG - chr3.4:85602283-85602302 MS.gene013437:intron 30.0%
AAAATGGTTGAGTGAGAGAT+AGG + chr3.4:85601932-85601951 None:intergenic 35.0%
CATCTGAGAATATACCAACA+AGG - chr3.4:85602538-85602557 MS.gene013437:intron 35.0%
CTGATTCTTCTTCTTCAAAG+TGG - chr3.4:85601797-85601816 MS.gene013437:CDS 35.0%
GGTGATTCTGTTTGATTGAA+TGG - chr3.4:85602039-85602058 MS.gene013437:CDS 35.0%
GTGATGACCAAATTCACTTT+GGG + chr3.4:85602311-85602330 None:intergenic 35.0%
TCTGATGTAAAACAACTGAC+AGG - chr3.4:85602711-85602730 MS.gene013437:CDS 35.0%
TGCTAACAAGTGACTATTTC+TGG - chr3.4:85602273-85602292 MS.gene013437:intron 35.0%
TGTGATGACCAAATTCACTT+TGG + chr3.4:85602312-85602331 None:intergenic 35.0%
!! TGATTGAATGGGCTTTTTGT+TGG - chr3.4:85602051-85602070 MS.gene013437:intron 35.0%
AAAAGCAGGTGTATGGAAGA+AGG - chr3.4:85602654-85602673 MS.gene013437:CDS 40.0%
AAAGCAGGTGTATGGAAGAA+GGG - chr3.4:85602655-85602674 MS.gene013437:CDS 40.0%
AAGTGGTGAACAGAACACTT+TGG - chr3.4:85602134-85602153 MS.gene013437:intron 40.0%
ACAGTGACAACTGCTGAAAA+TGG - chr3.4:85602081-85602100 MS.gene013437:intron 40.0%
CAGCACAACAATGATTGAGT+AGG + chr3.4:85602633-85602652 None:intergenic 40.0%
CAGTGACAACTGCTGAAAAT+GGG - chr3.4:85602082-85602101 MS.gene013437:intron 40.0%
CATTGTTGTGCTGAAAAAGC+AGG - chr3.4:85602640-85602659 MS.gene013437:intron 40.0%
CTCAAGAATTCCTCTTGCAA+AGG - chr3.4:85601898-85601917 MS.gene013437:intron 40.0%
CTTTGCAAGAGGAATTCTTG+AGG + chr3.4:85601900-85601919 None:intergenic 40.0%
TAACACTAACCACCCCAAAT+TGG - chr3.4:85602462-85602481 MS.gene013437:intron 40.0%
TCAGATGACATGATCACAAC+AGG + chr3.4:85602525-85602544 None:intergenic 40.0%
TCAGCAATCATCTGTGATGA+AGG - chr3.4:85602494-85602513 MS.gene013437:intron 40.0%
TCATCAACAGCAAGAACATG+AGG + chr3.4:85601844-85601863 None:intergenic 40.0%
TTCTTCTTCAAAGTGGGTGA+TGG - chr3.4:85601804-85601823 MS.gene013437:CDS 40.0%
! GAACAGAACACTTTGGAGAA+TGG - chr3.4:85602141-85602160 MS.gene013437:intron 40.0%
!! CTGAAGGCATTGGAGTATTT+GGG - chr3.4:85602105-85602124 MS.gene013437:intron 40.0%
!! TCAAAGTGGGTGATGGAAAA+TGG - chr3.4:85601811-85601830 MS.gene013437:CDS 40.0%
AAGCAGGTGTATGGAAGAAG+GGG - chr3.4:85602656-85602675 MS.gene013437:CDS 45.0%
ACAGGCCAGCTGATGAATTT+GGG - chr3.4:85602729-85602748 MS.gene013437:CDS 45.0%
ACCTTCCCAAATTCATCAGC+TGG + chr3.4:85602737-85602756 None:intergenic 45.0%
GAGTATTTGGGCTTGTCAAG+TGG - chr3.4:85602117-85602136 MS.gene013437:intron 45.0%
GCAATCATCTGTGATGAAGG+AGG - chr3.4:85602497-85602516 MS.gene013437:intron 45.0%
GTAGGGACCCAAAGTGAATT+TGG - chr3.4:85602301-85602320 MS.gene013437:intron 45.0%
GTGCTGAAAAAGCAGGTGTA+TGG - chr3.4:85602647-85602666 MS.gene013437:CDS 45.0%
!! AACTGCTGAAAATGGGCTGA+AGG - chr3.4:85602089-85602108 MS.gene013437:intron 45.0%
!! GCTGAAGGCATTGGAGTATT+TGG - chr3.4:85602104-85602123 MS.gene013437:intron 45.0%
!! TGAAAATGGGCTGAAGGCAT+TGG - chr3.4:85602095-85602114 MS.gene013437:intron 45.0%
AACTCATAGCCAGTCATCCC+TGG + chr3.4:85602347-85602366 None:intergenic 50.0%
ATCACAGACTACTGCATGCC+AGG - chr3.4:85602326-85602345 MS.gene013437:intron 50.0%
GACAGGCCAGCTGATGAATT+TGG - chr3.4:85602728-85602747 MS.gene013437:CDS 50.0%
GCCAGCTGATGAATTTGGGA+AGG - chr3.4:85602733-85602752 MS.gene013437:CDS 50.0%
GGTGTACGTACCTTTGCAAG+AGG + chr3.4:85601911-85601930 None:intergenic 50.0%
TCACAGACTACTGCATGCCA+GGG - chr3.4:85602327-85602346 MS.gene013437:intron 50.0%
TACTGCATGCCAGGGATGAC+TGG - chr3.4:85602335-85602354 MS.gene013437:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 85601790 85602770 85601790 ID=MS.gene013437
chr3.4 mRNA 85601790 85602770 85601790 ID=MS.gene013437.t1;Parent=MS.gene013437
chr3.4 exon 85602641 85602770 85602641 ID=MS.gene013437.t1.exon1;Parent=MS.gene013437.t1
chr3.4 CDS 85602641 85602770 85602641 ID=cds.MS.gene013437.t1;Parent=MS.gene013437.t1
chr3.4 exon 85602396 85602475 85602396 ID=MS.gene013437.t1.exon2;Parent=MS.gene013437.t1
chr3.4 CDS 85602396 85602475 85602396 ID=cds.MS.gene013437.t1;Parent=MS.gene013437.t1
chr3.4 exon 85602178 85602255 85602178 ID=MS.gene013437.t1.exon3;Parent=MS.gene013437.t1
chr3.4 CDS 85602178 85602255 85602178 ID=cds.MS.gene013437.t1;Parent=MS.gene013437.t1
chr3.4 exon 85601992 85602062 85601992 ID=MS.gene013437.t1.exon4;Parent=MS.gene013437.t1
chr3.4 CDS 85601992 85602062 85601992 ID=cds.MS.gene013437.t1;Parent=MS.gene013437.t1
chr3.4 exon 85601790 85601898 85601790 ID=MS.gene013437.t1.exon5;Parent=MS.gene013437.t1
chr3.4 CDS 85601790 85601898 85601790 ID=cds.MS.gene013437.t1;Parent=MS.gene013437.t1
Gene Sequence

>MS.gene013437

ATGGCTTCTGATTCTTCTTCTTCAAAGTGGGTGATGGAAAATGGTGACATTCCTCATGTTCTTGCTGTTGATGACAATATCATTGATCGCACACTTGTTGAGAAACTCCTCAAGAATTCCTCTTGCAAAGGTACGTACACCTATCTCTCACTCAACCATTTTCTGTTATATTCTTTTTCACTTTTTCAAACTAACTACTTTTACACTACATGCTTTTGATGTTTGATGTTTTTTTTTGGTCCATATTTTGGTGATTCTGTTTGATTGAATGGGCTTTTTGTTGGATACACTACAGTGACAACTGCTGAAAATGGGCTGAAGGCATTGGAGTATTTGGGCTTGTCAAGTGGTGAACAGAACACTTTGGAGAATGGAGTATGTTTTCTCTTTATGTGCATACATTTTACCAAAATGTTACTAACAAAACACACAATGACACTGAATTTGACATCAAATTTTGATGTTTAAAATTCATTTCTCACTTGCTAACAAGTGACTATTTCTGGTTTTTGTAGGGACCCAAAGTGAATTTGGTCATCACAGACTACTGCATGCCAGGGATGACTGGCTATGAGTTGCTTAAGAAAATTAAGGTACTTGCTGCAAAATCTTGTATATTACTATTTTTTTTTTCACAATACAGTATTGCTGCATTATATATATATGATATAGTAACACTAACCACCCCAAATTGGATATTATTTTCAGCAATCATCTGTGATGAAGGAGGTTCCTGTTGTGATCATGTCATCTGAGAATATACCAACAAGGATCAACAAGTAATTATTTGTCTCTAGCACTAACAATGCCATTTTTTTTGTATATTTTTCTTTTGTTTAACCTACTCAATCATTGTTGTGCTGAAAAAGCAGGTGTATGGAAGAAGGGGCTCAGATGTTCATACAAAAGCCTCTCAAACAATCTGATGTAAAACAACTGACAGGCCAGCTGATGAATTTGGGAAGGTTGCAAAAGAATTGA

Protein sequence

>MS.gene013437.t1

MASDSSSSKWVMENGDIPHVLAVDDNIIDRTLVEKLLKNSSCKVTTAENGLKALEYLGLSSGEQNTLENGGPKVNLVITDYCMPGMTGYELLKKIKQSSVMKEVPVVIMSSENIPTRINKCMEEGAQMFIQKPLKQSDVKQLTGQLMNLGRLQKN