AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013532.t1 MTR_3g092150 98.367 245 4 0 1 245 1 245 0.0 496
MS.gene013532.t1 MTR_8g026960 43.750 208 109 4 42 245 38 241 5.02e-48 159
MS.gene013532.t1 MTR_6g007647 45.185 135 63 2 7 130 25 159 3.92e-30 114
MS.gene013532.t1 MTR_6g007647 57.447 94 40 0 37 130 32 125 4.30e-30 113
MS.gene013532.t1 MTR_4g107650 56.122 98 43 0 37 134 94 191 4.73e-29 112
MS.gene013532.t1 MTR_6g011610 56.383 94 41 0 37 130 95 188 8.36e-29 110
MS.gene013532.t1 MTR_6g011610 56.383 94 41 0 37 130 76 169 9.40e-29 109
MS.gene013532.t1 MTR_8g468210 54.255 94 43 0 37 130 76 169 4.50e-28 108
MS.gene013532.t1 MTR_1g061660 49.533 107 46 2 37 142 58 157 1.35e-26 103
MS.gene013532.t1 MTR_5g038280 55.435 92 41 0 39 130 88 179 2.04e-26 104
MS.gene013532.t1 MTR_8g089895 55.435 92 41 0 39 130 55 146 2.49e-26 104
MS.gene013532.t1 MTR_5g039000 54.255 94 43 0 37 130 76 169 3.08e-26 103
MS.gene013532.t1 MTR_3g086790 51.064 94 46 0 37 130 59 152 6.69e-26 103
MS.gene013532.t1 MTR_7g103340 40.690 145 58 4 37 160 62 199 3.37e-25 99.8
MS.gene013532.t1 MTR_8g469430 54.348 92 42 0 39 130 90 181 5.26e-25 100
MS.gene013532.t1 MTR_3g080100 49.474 95 48 0 36 130 18 112 5.97e-25 98.6
MS.gene013532.t1 MTR_3g080100 49.474 95 48 0 36 130 57 151 9.64e-25 99.0
MS.gene013532.t1 MTR_7g010020 47.826 92 48 0 39 130 81 172 3.25e-21 89.7
MS.gene013532.t1 MTR_5g019680 47.368 114 46 2 31 130 39 152 5.76e-21 87.8
MS.gene013532.t1 MTR_5g019650 52.577 97 38 2 31 120 39 134 1.79e-19 84.3
MS.gene013532.t1 MTR_4g100550 44.211 95 46 1 39 133 121 208 9.74e-14 69.3
MS.gene013532.t1 MTR_7g093430 41.584 101 52 1 33 133 155 248 9.45e-13 66.6
MS.gene013532.t1 MTR_4g126900 42.857 84 48 0 41 124 91 174 1.79e-12 65.1
MS.gene013532.t1 MTR_4g097600 43.158 95 47 1 39 133 128 215 2.91e-12 65.1
MS.gene013532.t1 MTR_5g014890 43.750 96 53 1 39 133 124 219 5.18e-12 64.3
MS.gene013532.t1 MTR_1g017090 43.158 95 47 1 39 133 131 218 8.64e-12 63.9
MS.gene013532.t1 MTR_1g054285 39.423 104 56 1 33 136 149 245 1.42e-11 63.5
MS.gene013532.t1 MTR_3g103590 45.333 75 41 0 36 110 143 217 1.60e-11 63.2
MS.gene013532.t1 MTR_8g006705 42.105 95 48 1 39 133 147 234 2.04e-11 63.2
MS.gene013532.t1 MTR_7g027265 31.250 144 73 4 35 177 17 135 2.37e-11 60.5
MS.gene013532.t1 MTR_2g061030 40.000 95 50 1 39 133 201 288 7.45e-11 61.6
MS.gene013532.t1 MTR_5g013010 41.053 95 49 1 39 133 137 224 8.84e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013532.t1 AT2G46680 42.324 241 118 5 25 245 19 258 1.98e-45 152
MS.gene013532.t1 AT2G46680 43.162 234 114 5 30 245 24 256 2.14e-44 150
MS.gene013532.t1 AT3G61890 68.132 91 29 0 41 131 33 123 2.00e-38 134
MS.gene013532.t1 AT3G01470 43.382 136 77 0 37 172 67 202 1.14e-29 112
MS.gene013532.t1 AT2G22430 56.383 94 41 0 37 130 61 154 4.87e-29 111
MS.gene013532.t1 AT4G40060 52.041 98 47 0 37 134 58 155 2.13e-28 109
MS.gene013532.t1 AT1G69780 54.255 94 43 0 37 130 84 177 8.92e-28 107
MS.gene013532.t1 AT5G15150 53.191 94 44 0 37 130 114 207 3.48e-26 103
MS.gene013532.t1 AT2G18550 50.943 106 45 1 39 137 62 167 8.26e-26 100
MS.gene013532.t1 AT5G65310 51.064 94 46 0 37 130 53 146 2.88e-25 101
MS.gene013532.t1 AT5G65310 51.064 94 46 0 37 130 71 164 3.70e-25 101
MS.gene013532.t1 AT4G36740 42.647 136 61 2 17 135 25 160 9.24e-25 98.2
MS.gene013532.t1 AT5G66700 51.000 100 42 1 39 131 72 171 1.32e-24 98.2
MS.gene013532.t1 AT4G36740 50.962 104 44 1 39 135 56 159 1.33e-24 97.8
MS.gene013532.t1 AT3G01220 48.936 94 48 0 37 130 86 179 8.91e-24 97.1
MS.gene013532.t1 AT5G03790 43.478 115 54 2 20 134 63 166 1.85e-23 95.1
MS.gene013532.t1 AT1G26960 42.969 128 65 2 11 130 36 163 1.70e-22 93.2
MS.gene013532.t1 AT5G03790 46.000 100 50 1 20 119 63 158 7.68e-22 89.7
MS.gene013532.t1 AT1G27045 40.299 134 74 2 9 138 35 166 4.53e-21 89.4
MS.gene013532.t1 AT1G27045 39.683 126 72 1 9 130 35 160 5.48e-20 85.9
MS.gene013532.t1 AT1G27045 45.361 97 53 0 34 130 21 117 5.99e-20 84.7
MS.gene013532.t1 AT1G27045 45.361 97 53 0 34 130 21 117 5.99e-20 84.7
MS.gene013532.t1 AT1G27045 45.361 97 53 0 34 130 13 109 8.17e-20 84.0
MS.gene013532.t1 AT2G36610 42.308 104 45 2 39 134 72 168 2.58e-19 82.8
MS.gene013532.t1 AT5G53980 39.604 101 57 1 29 129 6 102 1.42e-15 72.0
MS.gene013532.t1 AT2G44910 42.000 100 51 1 39 138 164 256 1.46e-12 66.6
MS.gene013532.t1 AT3G60390 42.000 100 51 1 39 138 163 255 3.50e-12 65.5
MS.gene013532.t1 AT2G22800 42.105 95 48 1 39 133 114 201 1.10e-11 63.5
MS.gene013532.t1 AT5G06710 41.837 98 50 1 33 130 185 275 1.37e-11 63.9
MS.gene013532.t1 AT4G16780 42.105 95 48 1 39 133 130 217 5.85e-11 61.6
MS.gene013532.t1 AT4G37790 41.053 95 49 1 39 133 127 214 8.32e-11 61.2
MS.gene013532.t1 AT2G01430 42.105 95 48 1 39 133 140 227 8.71e-11 60.8

Find 37 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTCATGAAGGGTTTAA+AGG 0.209558 3.4:-84331619 MS.gene013532:CDS
AGCCTAGACAAGTTGCTATA+TGG 0.329458 3.4:-84331414 MS.gene013532:CDS
GCAAGTTGCATCTTCTTCCT+TGG 0.339735 3.4:+84331454 None:intergenic
TGAGGGTTTGGAGGATAAAA+TGG 0.360194 3.4:-84330872 MS.gene013532:CDS
AGCACAGAAGATGGTGGTTC+AGG 0.365057 3.4:-84330946 MS.gene013532:CDS
GGTTCAGAAGCTAAGTTATA+TGG 0.367405 3.4:-84331010 MS.gene013532:CDS
GAAGATGGTGGTTCAGGAAA+TGG 0.372815 3.4:-84330940 MS.gene013532:CDS
GAAATGGATACAACAATTGC+AGG 0.379643 3.4:-84330924 MS.gene013532:CDS
ATGCAACTTGCAAGAGATCT+TGG 0.385707 3.4:-84331442 MS.gene013532:CDS
TTCTAAGTTTCAGTGCCTTA+AGG 0.396105 3.4:-84331308 MS.gene013532:CDS
AAGACTCTTTCTCTTCCTTA+AGG 0.407960 3.4:+84331293 None:intergenic
TGATGAACAGATAAGATCAT+TGG 0.455417 3.4:-84331509 MS.gene013532:CDS
ATGCAATAGTTCTCAATGGT+TGG 0.475551 3.4:-84330686 MS.gene013532:CDS
GAAGAAGAGAATATTCATGA+AGG 0.503417 3.4:-84331628 MS.gene013532:CDS
CAGAATCAAAGTTGGAGCCA+AGG 0.518559 3.4:-84331471 MS.gene013532:CDS
ATTTGAGTCAGAATCAAAGT+TGG 0.518777 3.4:-84331479 MS.gene013532:CDS
AAGAAGAGAATATTCATGAA+GGG 0.519573 3.4:-84331627 MS.gene013532:CDS
AAGGAAAATAGCACAGAAGA+TGG 0.540540 3.4:-84330955 MS.gene013532:CDS
GAAAGAGTCTTTGCAATCAG+AGG 0.541327 3.4:-84331281 MS.gene013532:intron
AATCATGCAATAGTTCTCAA+TGG 0.553811 3.4:-84330690 MS.gene013532:CDS
AGAAGATAGAAAACAAGAGA+AGG 0.569555 3.4:-84331537 MS.gene013532:CDS
AAACATCGCATGATGGAGGA+AGG 0.574577 3.4:-84330984 MS.gene013532:CDS
AACCATATAGCAACTTGTCT+AGG 0.580258 3.4:+84331412 None:intergenic
TTCAGAACAGAAGAGCAAGA+TGG 0.602218 3.4:-84331390 MS.gene013532:CDS
TGATGGAGGAAGGGAAGCTA+AGG 0.606332 3.4:-84330974 MS.gene013532:CDS
TCAGAAGCTAAGTTATATGG+TGG 0.615960 3.4:-84331007 MS.gene013532:CDS
ATGGTGGAAACATCGCATGA+TGG 0.617224 3.4:-84330991 MS.gene013532:CDS
AACATCGCATGATGGAGGAA+GGG 0.628539 3.4:-84330983 MS.gene013532:CDS
GTGGAAACATCGCATGATGG+AGG 0.645127 3.4:-84330988 MS.gene013532:CDS
GGCCATATATAATATGATGG+AGG 0.645919 3.4:-84331650 None:intergenic
GAGCAAGATGGAAATCAAAG+AGG 0.650346 3.4:-84331378 MS.gene013532:CDS
GATTGATCTAACATACCACA+TGG 0.663576 3.4:+84330709 None:intergenic
GAAAATAGCACAGAAGATGG+TGG 0.663671 3.4:-84330952 MS.gene013532:CDS
AAAATGAAAGAAGCACAACA+TGG 0.668449 3.4:-84330831 MS.gene013532:CDS
ACAGAGAAAATTGAGGACAG+AGG 0.671559 3.4:-84330808 MS.gene013532:CDS
AACATGGACAGAGAAAATTG+AGG 0.710156 3.4:-84330815 MS.gene013532:CDS
TGGTACAATGTTGATCCATG+TGG 0.723453 3.4:-84330724 MS.gene013532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAAGAGAATATTCATGAA+GGG - chr3.4:84330682-84330701 MS.gene013532:CDS 25.0%
! AATATTCATGAAGGGTTTAA+AGG - chr3.4:84330690-84330709 MS.gene013532:CDS 25.0%
! TCAACTCATTTAATTAATCG+AGG + chr3.4:84331222-84331241 None:intergenic 25.0%
!! AAAGCAAAGATTTTCAAATG+AGG - chr3.4:84331419-84331438 MS.gene013532:CDS 25.0%
!! AAGCAAAGATTTTCAAATGA+GGG - chr3.4:84331420-84331439 MS.gene013532:CDS 25.0%
!! TTTTTCTTCTTTGAATTCTG+AGG + chr3.4:84330750-84330769 None:intergenic 25.0%
AAGTTAATGTCGAGTCTATA+AGG + chr3.4:84331252-84331271 None:intergenic 30.0%
AATCATGCAATAGTTCTCAA+TGG - chr3.4:84331619-84331638 MS.gene013532:CDS 30.0%
AGAAGATAGAAAACAAGAGA+AGG - chr3.4:84330772-84330791 MS.gene013532:CDS 30.0%
ATTTGAGTCAGAATCAAAGT+TGG - chr3.4:84330830-84330849 MS.gene013532:CDS 30.0%
GAAGAAGAGAATATTCATGA+AGG - chr3.4:84330681-84330700 MS.gene013532:CDS 30.0%
GTCAGGAAAAAAAAATTCAC+AGG + chr3.4:84331278-84331297 None:intergenic 30.0%
TGACAACAACACTTGAAAAA+TGG - chr3.4:84331565-84331584 MS.gene013532:CDS 30.0%
TGATGAACAGATAAGATCAT+TGG - chr3.4:84330800-84330819 MS.gene013532:CDS 30.0%
! AAAATGAAAGAAGCACAACA+TGG - chr3.4:84331478-84331497 MS.gene013532:CDS 30.0%
! AAGATTTTCAAATGAGGGTT+TGG - chr3.4:84331425-84331444 MS.gene013532:CDS 30.0%
!!! GTGAATTTTTTTTTCCTGAC+AGG - chr3.4:84331278-84331297 MS.gene013532:intron 30.0%
!!! TTTTCAAGTGTTGTTGTCAA+GGG + chr3.4:84331564-84331583 None:intergenic 30.0%
!!! TTTTTCAAGTGTTGTTGTCA+AGG + chr3.4:84331565-84331584 None:intergenic 30.0%
AACATGGACAGAGAAAATTG+AGG - chr3.4:84331494-84331513 MS.gene013532:CDS 35.0%
AACCATATAGCAACTTGTCT+AGG + chr3.4:84330900-84330919 None:intergenic 35.0%
AAGACTCTTTCTCTTCCTTA+AGG + chr3.4:84331019-84331038 None:intergenic 35.0%
AAGGAAAATAGCACAGAAGA+TGG - chr3.4:84331354-84331373 MS.gene013532:CDS 35.0%
ATGCAATAGTTCTCAATGGT+TGG - chr3.4:84331623-84331642 MS.gene013532:CDS 35.0%
GAAATGGATACAACAATTGC+AGG - chr3.4:84331385-84331404 MS.gene013532:CDS 35.0%
GATTGATCTAACATACCACA+TGG + chr3.4:84331603-84331622 None:intergenic 35.0%
GGTTCAGAAGCTAAGTTATA+TGG - chr3.4:84331299-84331318 MS.gene013532:CDS 35.0%
TCAGAAGCTAAGTTATATGG+TGG - chr3.4:84331302-84331321 MS.gene013532:CDS 35.0%
TCAGAGGTAAGTTAGTAGTT+TGG - chr3.4:84331044-84331063 MS.gene013532:intron 35.0%
TTCTAAGTTTCAGTGCCTTA+AGG - chr3.4:84331001-84331020 MS.gene013532:CDS 35.0%
! ATTTTCAAATGAGGGTTTGG+AGG - chr3.4:84331428-84331447 MS.gene013532:CDS 35.0%
! GTTCTCAATGGTTGGATTTT+TGG - chr3.4:84331631-84331650 MS.gene013532:CDS 35.0%
ACAGAGAAAATTGAGGACAG+AGG - chr3.4:84331501-84331520 MS.gene013532:CDS 40.0%
AGCCTAGACAAGTTGCTATA+TGG - chr3.4:84330895-84330914 MS.gene013532:CDS 40.0%
ATGCAACTTGCAAGAGATCT+TGG - chr3.4:84330867-84330886 MS.gene013532:CDS 40.0%
GAAAATAGCACAGAAGATGG+TGG - chr3.4:84331357-84331376 MS.gene013532:CDS 40.0%
GAAAGAGTCTTTGCAATCAG+AGG - chr3.4:84331028-84331047 MS.gene013532:intron 40.0%
GACTCAGCTCGTTAAATACT+CGG - chr3.4:84331149-84331168 MS.gene013532:intron 40.0%
GAGCAAGATGGAAATCAAAG+AGG - chr3.4:84330931-84330950 MS.gene013532:CDS 40.0%
TGAGGGTTTGGAGGATAAAA+TGG - chr3.4:84331437-84331456 MS.gene013532:CDS 40.0%
TGGTACAATGTTGATCCATG+TGG - chr3.4:84331585-84331604 MS.gene013532:CDS 40.0%
TTCAGAACAGAAGAGCAAGA+TGG - chr3.4:84330919-84330938 MS.gene013532:CDS 40.0%
ACTTAGCTTCTGAACCTGTC+AGG + chr3.4:84331295-84331314 None:intergenic 45.0%
CAGAATCAAAGTTGGAGCCA+AGG - chr3.4:84330838-84330857 MS.gene013532:CDS 45.0%
GCAAGTTGCATCTTCTTCCT+TGG + chr3.4:84330858-84330877 None:intergenic 45.0%
! AAACATCGCATGATGGAGGA+AGG - chr3.4:84331325-84331344 MS.gene013532:CDS 45.0%
! AACATCGCATGATGGAGGAA+GGG - chr3.4:84331326-84331345 MS.gene013532:CDS 45.0%
! ATGGTGGAAACATCGCATGA+TGG - chr3.4:84331318-84331337 MS.gene013532:CDS 45.0%
! GAAGATGGTGGTTCAGGAAA+TGG - chr3.4:84331369-84331388 MS.gene013532:CDS 45.0%
GTGGAAACATCGCATGATGG+AGG - chr3.4:84331321-84331340 MS.gene013532:CDS 50.0%
TGATGGAGGAAGGGAAGCTA+AGG - chr3.4:84331335-84331354 MS.gene013532:CDS 50.0%
! AGCACAGAAGATGGTGGTTC+AGG - chr3.4:84331363-84331382 MS.gene013532:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 84330672 84331659 84330672 ID=MS.gene013532
chr3.4 mRNA 84330672 84331659 84330672 ID=MS.gene013532.t1;Parent=MS.gene013532
chr3.4 exon 84331282 84331659 84331282 ID=MS.gene013532.t1.exon1;Parent=MS.gene013532.t1
chr3.4 CDS 84331282 84331659 84331282 ID=cds.MS.gene013532.t1;Parent=MS.gene013532.t1
chr3.4 exon 84330672 84331031 84330672 ID=MS.gene013532.t1.exon2;Parent=MS.gene013532.t1
chr3.4 CDS 84330672 84331031 84330672 ID=cds.MS.gene013532.t1;Parent=MS.gene013532.t1
Gene Sequence

>MS.gene013532

ATGATGGAGGAAGAAGAGAATATTCATGAAGGGTTTAAAGGAAAAAATGTTGAAAGCATGAGTTGCTTAGAGACACCTCAGAATTCAAAGAAGAAAAACAAGAAGATAGAAAACAAGAGAAGGTTTAGTGATGAACAGATAAGATCATTGGAATGCATATTTGAGTCAGAATCAAAGTTGGAGCCAAGGAAGAAGATGCAACTTGCAAGAGATCTTGGTTTGCAGCCTAGACAAGTTGCTATATGGTTTCAGAACAGAAGAGCAAGATGGAAATCAAAGAGGATAGAACAAGAATACAGAAAACTCAAAGATGAATATGATAATTTAGCTTCTAAGTTTCAGTGCCTTAAGGAAGAGAAAGAGTCTTTGCAATCAGAGGTAAGTTAGTAGTTTGGTTCTTGTAATTGTAACATAACTAGAGTTGTGTCTCGATAAACAGCTTAATTTAAACGAGTTGAGCTATTCGTGAACTATTCAGACTCAGCTCGTTAAATACTCGGTTATGTTCGTTTGTTTAGTTAAATAACATGCTCAAGCTTAGTATTAGCCTCGATTAATTAAATGAGTTGAACTTAAACCTTATAGACTCGACATTAACTTAAACCTGTGAATTTTTTTTTCCTGACAGGTTCAGAAGCTAAGTTATATGGTGGAAACATCGCATGATGGAGGAAGGGAAGCTAAGGAAAATAGCACAGAAGATGGTGGTTCAGGAAATGGATACAACAATTGCAGGTTTGAAGAAACAAAGCAAAGATTTTCAAATGAGGGTTTGGAGGATAAAATGGTCATTTATTCAGATGATCAAAATGAAAGAAGCACAACATGGACAGAGAAAATTGAGGACAGAGGAAATCAACTTTTAAGAAATGATGATCATGCAGAAATTCCCTTGACAACAACACTTGAAAAATGGTACAATGTTGATCCATGTGGTATGTTAGATCAATCATGCAATAGTTCTCAATGGTTGGATTTTTGGACTTGA

Protein sequence

>MS.gene013532.t1

MMEEEENIHEGFKGKNVESMSCLETPQNSKKKNKKIENKRRFSDEQIRSLECIFESESKLEPRKKMQLARDLGLQPRQVAIWFQNRRARWKSKRIEQEYRKLKDEYDNLASKFQCLKEEKESLQSEVQKLSYMVETSHDGGREAKENSTEDGGSGNGYNNCRFEETKQRFSNEGLEDKMVIYSDDQNERSTTWTEKIEDRGNQLLRNDDHAEIPLTTTLEKWYNVDPCGMLDQSCNSSQWLDFWT