AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013631

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013631.t1 MTR_3g090860 96.471 340 8 1 1 336 1 340 0.0 675
MS.gene013631.t1 MTR_8g099350 36.512 367 179 16 7 336 12 361 5.55e-49 168
MS.gene013631.t1 MTR_7g117200 34.366 355 169 15 7 336 12 327 2.14e-45 157
MS.gene013631.t1 MTR_8g027860 35.278 360 168 16 7 330 12 342 2.10e-41 147
MS.gene013631.t1 MTR_7g073430 32.353 204 100 5 2 193 9 186 3.20e-21 92.0
MS.gene013631.t1 MTR_7g073380 31.472 197 95 6 7 190 15 184 3.07e-20 89.7
MS.gene013631.t1 MTR_5g067680 59.375 64 22 1 129 188 198 261 3.85e-19 87.4
MS.gene013631.t1 MTR_8g005750 31.364 220 118 7 8 223 6 196 5.22e-17 80.1
MS.gene013631.t1 MTR_3g093830 31.336 217 113 10 13 217 23 215 1.01e-16 80.1
MS.gene013631.t1 MTR_0002s1250 28.000 200 114 5 1 194 1 176 2.02e-16 78.2
MS.gene013631.t1 MTR_5g067700 39.326 89 50 1 104 188 77 165 8.19e-14 70.5
MS.gene013631.t1 MTR_2g075750 35.417 96 58 1 132 223 27 122 1.32e-13 68.6
MS.gene013631.t1 MTR_2g075690 29.268 205 106 8 5 188 14 200 2.18e-13 70.1
MS.gene013631.t1 MTR_5g073620 39.024 82 44 2 125 202 664 743 4.25e-13 70.9
MS.gene013631.t1 MTR_2g075700 37.500 88 43 2 105 188 124 203 1.23e-12 68.2
MS.gene013631.t1 MTR_2g075680 29.557 203 115 6 5 188 14 207 1.81e-12 67.4
MS.gene013631.t1 MTR_2g075610 29.545 132 80 3 132 259 28 150 2.04e-12 65.5
MS.gene013631.t1 MTR_8g098945 46.774 62 31 1 127 186 123 184 4.32e-12 65.9
MS.gene013631.t1 MTR_7g079010 33.094 139 79 4 67 199 80 210 6.67e-12 64.7
MS.gene013631.t1 MTR_8g028565 37.634 93 53 2 134 221 156 248 1.05e-11 65.5
MS.gene013631.t1 MTR_7g079040 31.707 164 103 4 126 280 147 310 3.70e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013631.t1 AT4G11070 35.417 336 151 13 3 318 7 296 3.77e-42 149
MS.gene013631.t1 AT4G23810 36.296 270 130 11 3 244 10 265 1.14e-41 148
MS.gene013631.t1 AT5G24110 39.286 224 96 9 5 219 14 206 3.11e-38 138
MS.gene013631.t1 AT4G11070 32.845 341 151 13 3 332 7 280 1.14e-35 131
MS.gene013631.t1 AT2G46400 30.030 333 160 13 1 315 2 279 7.80e-27 107
MS.gene013631.t1 AT2G40750 31.556 225 115 9 5 203 13 224 1.22e-21 94.4
MS.gene013631.t1 AT3G56400 35.079 191 93 8 7 188 11 179 2.93e-21 92.4
MS.gene013631.t1 AT2G40740 56.452 62 23 1 131 188 171 232 1.15e-17 82.4
MS.gene013631.t1 AT2G40740 56.452 62 23 1 131 188 180 241 1.41e-17 82.4
MS.gene013631.t1 AT1G66550 28.649 185 96 5 8 188 10 162 3.22e-13 68.9
MS.gene013631.t1 AT1G66550 27.083 192 95 5 8 188 10 167 3.43e-13 68.9
MS.gene013631.t1 AT2G46130 45.714 70 33 2 132 199 29 95 4.32e-13 65.1
MS.gene013631.t1 AT1G66600 28.571 203 102 6 8 201 10 178 1.01e-12 67.4
MS.gene013631.t1 AT5G01900 28.293 205 118 6 8 207 9 189 1.03e-12 67.8
MS.gene013631.t1 AT5G49520 50.877 57 27 1 132 187 220 276 1.80e-12 68.2
MS.gene013631.t1 AT5G52830 55.357 56 23 1 134 187 166 221 8.18e-12 65.9
MS.gene013631.t1 AT5G41570 45.070 71 36 2 132 199 97 167 8.89e-12 63.5
MS.gene013631.t1 AT5G45260 43.902 82 39 3 112 186 1184 1265 1.91e-11 65.9
MS.gene013631.t1 AT2G46130 43.662 71 37 2 132 199 29 99 1.95e-11 60.8
MS.gene013631.t1 AT1G64000 38.000 100 53 4 104 199 89 183 2.13e-11 62.8
MS.gene013631.t1 AT5G07100 39.130 92 49 3 115 199 210 301 2.90e-11 63.9
MS.gene013631.t1 AT5G07100 39.130 92 49 3 115 199 117 208 4.34e-11 62.4
MS.gene013631.t1 AT5G07100 39.130 92 49 3 115 199 117 208 4.34e-11 62.4
MS.gene013631.t1 AT5G07100 39.130 92 49 3 115 199 117 208 4.34e-11 62.4
MS.gene013631.t1 AT5G07100 39.130 92 49 3 115 199 117 208 4.34e-11 62.4
MS.gene013631.t1 AT2G03340 32.903 155 82 8 45 186 158 303 5.46e-11 63.9

Find 77 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATCTCACTTGCCATATT+TGG 0.088426 3.4:+83182378 None:intergenic
CAAATTTACATACCTCTTTC+TGG 0.143241 3.4:+83183327 None:intergenic
CCGTAAAAGACATGTTAATA+TGG 0.191166 3.4:-83183482 MS.gene013631:CDS
TCCAAGTGTGTTCCCAAATA+TGG 0.244585 3.4:-83182391 MS.gene013631:CDS
TTTGATCAATTTGAATTTGA+TGG 0.254177 3.4:-83182303 MS.gene013631:CDS
TGTTAACAATATTTGTTTCT+TGG 0.254224 3.4:+83182637 None:intergenic
TATGTTTCCCCTGCAAATTC+AGG 0.272093 3.4:-83182441 MS.gene013631:CDS
ATGGAACAAATGATTGGTTT+AGG 0.306745 3.4:+83182517 None:intergenic
AATCTCACGTGTTTCATTAG+AGG 0.318717 3.4:+83183555 None:intergenic
GTTAACAATATTTGTTTCTT+GGG 0.321184 3.4:+83182638 None:intergenic
ATGATTGGTTTAGGAAATCT+AGG 0.323422 3.4:+83182526 None:intergenic
GGACCTTCCACTGCCATTCC+TGG 0.341337 3.4:+83183001 None:intergenic
TGACTTATTCCTGAATTTGC+AGG 0.343271 3.4:+83182432 None:intergenic
GGAACACTCTACCAATTAGA+TGG 0.350756 3.4:-83183045 MS.gene013631:CDS
TTTGTTTCTTGGGTTTGAAA+TGG 0.354628 3.4:+83182648 None:intergenic
TAATCTCACTTGCCATATTT+GGG 0.361487 3.4:+83182379 None:intergenic
TTTCAAATGGAACAAATGAT+TGG 0.363750 3.4:+83182511 None:intergenic
GGGTATAGTTGGAGAAAATA+TGG 0.366566 3.4:-83182968 MS.gene013631:CDS
ATTTGAAAAGGCACTTGAAA+TGG 0.370230 3.4:-83183509 MS.gene013631:CDS
TGCACTCATAGAAACGTTCT+AGG 0.387757 3.4:-83182792 MS.gene013631:CDS
TCAAATTCAAATTGATCAAA+TGG 0.396441 3.4:+83182306 None:intergenic
TAGAAACGTTCTAGGGTGTT+TGG 0.404066 3.4:-83182784 MS.gene013631:CDS
TTCAAAAGTTGTTCATTTGG+TGG 0.409814 3.4:+83182585 None:intergenic
CAAAGCTACTTTCAAGTACA+TGG 0.413007 3.4:+83182487 None:intergenic
TTGTTCAAAAGTTGTTCATT+TGG 0.416222 3.4:+83182582 None:intergenic
AAACACACCAACTGCTTCAA+TGG 0.437704 3.4:-83182334 MS.gene013631:CDS
AGTTGTGGAATGTGAAGTTT+TGG 0.442534 3.4:+83182280 None:intergenic
GCCATATTTGGGAACACACT+TGG 0.442960 3.4:+83182390 None:intergenic
CTGAGGCTGAAGGGTATTGT+TGG 0.449383 3.4:+83183446 None:intergenic
TATGTGATTTCAAAGATAGT+TGG 0.452782 3.4:+83182729 None:intergenic
CGTAAAAGACATGTTAATAT+GGG 0.457646 3.4:-83183481 MS.gene013631:CDS
TGAAATGGATTAGTTAATGT+TGG 0.458156 3.4:+83182663 None:intergenic
CTCTTGATGATGGGTATAGT+TGG 0.464883 3.4:-83182979 MS.gene013631:CDS
AAGGTGGCGAATCTGATATT+CGG 0.465518 3.4:+83183410 None:intergenic
TTTGTTGATCAAGGCTTTGA+GGG 0.467796 3.4:+83182610 None:intergenic
AATTATATCCACATTTGAAA+AGG 0.478087 3.4:-83183521 MS.gene013631:CDS
AGATAAGCTACTACAAGGAT+TGG 0.478321 3.4:-83183620 MS.gene013631:CDS
GTGGAAGGTCCTCTTGATGA+TGG 0.481430 3.4:-83182989 MS.gene013631:CDS
TTCTGGAAGCATTGTGATCA+CGG 0.489343 3.4:+83183344 None:intergenic
ATGTGATTTCAAAGATAGTT+GGG 0.493028 3.4:+83182730 None:intergenic
CAACAATACCCTTCAGCCTC+AGG 0.495514 3.4:-83183445 MS.gene013631:CDS
ATCACAATGCTTCCAGAAAG+AGG 0.506811 3.4:-83183339 MS.gene013631:intron
TTTGGGAACACACTTGGAGT+TGG 0.510977 3.4:+83182396 None:intergenic
GACTTATTCCTGAATTTGCA+GGG 0.514420 3.4:+83182433 None:intergenic
ACCTTGGATGCACGGCTCCA+AGG 0.515275 3.4:+83182933 None:intergenic
TCCTTGGAGCCGTGCATCCA+AGG 0.519224 3.4:-83182934 MS.gene013631:intron
CTGTCGGAGTCTTCACTTCG+CGG 0.524147 3.4:+83183376 None:intergenic
TTGTGCATGTGTGGTTTCCT+CGG 0.524687 3.4:+83182706 None:intergenic
TATGGTCAGAAAGACATCCT+TGG 0.530226 3.4:-83182950 MS.gene013631:CDS
GCAAGTGTTCCTGAGGCTGA+AGG 0.534387 3.4:+83183436 None:intergenic
GTGTTTGCAGCAGAAGTAGC+TGG 0.543085 3.4:+83182351 None:intergenic
CAAGTGTTCCTGAGGCTGAA+GGG 0.573717 3.4:+83183437 None:intergenic
GCTACTACAAGGATTGGAGT+TGG 0.576309 3.4:-83183614 MS.gene013631:CDS
AATTATATACCTTGGATGCA+CGG 0.579367 3.4:+83182925 None:intergenic
GGAAACTCCATTGAAGCAGT+TGG 0.580486 3.4:+83182327 None:intergenic
TGGAAGGTCCTCTTGATGAT+GGG 0.583275 3.4:-83182988 MS.gene013631:CDS
TATTCGAGTTGAACCAGGAA+TGG 0.589228 3.4:-83183014 MS.gene013631:CDS
AGTTGAACCAGGAATGGCAG+TGG 0.591297 3.4:-83183008 MS.gene013631:CDS
TTTGCAGGGGAAACATATGA+AGG 0.596062 3.4:+83182447 None:intergenic
ATCACGGTTAAAATCTCTGT+CGG 0.596477 3.4:+83183360 None:intergenic
GAACCAGGAATGGCAGTGGA+AGG 0.596663 3.4:-83183004 MS.gene013631:CDS
TTAATAGATAAGCTACTACA+AGG 0.612960 3.4:-83183625 MS.gene013631:CDS
AAACATATTCGAGTTGAACC+AGG 0.613692 3.4:-83183019 MS.gene013631:CDS
GAACTCTTAGACCTGTTCGT+AGG 0.617613 3.4:+83182559 None:intergenic
CTTCGCGGACTACAGCTCAA+AGG 0.617709 3.4:+83183391 None:intergenic
TAATGTTGGACCAACCACAT+TGG 0.618042 3.4:+83182677 None:intergenic
CAACTATACCCATCATCAAG+AGG 0.622097 3.4:+83182980 None:intergenic
TGGAAGCAATTGTGCATGTG+TGG 0.623042 3.4:+83182697 None:intergenic
ACAATTGCTTCCAATGTGGT+TGG 0.627124 3.4:-83182687 MS.gene013631:CDS
GCACTCATAGAAACGTTCTA+GGG 0.632178 3.4:-83182791 MS.gene013631:CDS
TGGCGAATCTGATATTCGGA+TGG 0.634201 3.4:+83183414 None:intergenic
ATCTTTGAAATCACATACCG+AGG 0.643767 3.4:-83182723 MS.gene013631:CDS
CGCGGACTACAGCTCAAAGG+TGG 0.645554 3.4:+83183394 None:intergenic
TCGGATGGCAAGTGTTCCTG+AGG 0.646934 3.4:+83183429 None:intergenic
ACTTATTCCTGAATTTGCAG+GGG 0.668793 3.4:+83182434 None:intergenic
ATGCACAATTGCTTCCAATG+TGG 0.717502 3.4:-83182691 MS.gene013631:CDS
TTTGCAGCAGAAGTAGCTGG+TGG 0.728180 3.4:+83182354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATATTTATTTTTTTT+AGG - chr3.4:83182840-83182859 MS.gene013631:intron 0.0%
!! AAATATAAAAACTAGGGTAA+AGG + chr3.4:83182831-83182850 None:intergenic 20.0%
!! AATTATATCCACATTTGAAA+AGG - chr3.4:83182385-83182404 MS.gene013631:CDS 20.0%
!! AATTGAGAAATTATATACCT+TGG + chr3.4:83182992-83183011 None:intergenic 20.0%
!! TCAAATTCAAATTGATCAAA+TGG + chr3.4:83183603-83183622 None:intergenic 20.0%
!! TTTGATCAATTTGAATTTGA+TGG - chr3.4:83183603-83183622 MS.gene013631:CDS 20.0%
!!! AAGTTTTTTTTAATACCGTA+GGG - chr3.4:83182665-83182684 MS.gene013631:CDS 20.0%
!!! GTTAACAATATTTGTTTCTT+GGG + chr3.4:83183271-83183290 None:intergenic 20.0%
!!! TGTTAACAATATTTGTTTCT+TGG + chr3.4:83183272-83183291 None:intergenic 20.0%
! ATGTGATTTCAAAGATAGTT+GGG + chr3.4:83183179-83183198 None:intergenic 25.0%
! CGTAAAAGACATGTTAATAT+GGG - chr3.4:83182425-83182444 MS.gene013631:CDS 25.0%
! TATGTGATTTCAAAGATAGT+TGG + chr3.4:83183180-83183199 None:intergenic 25.0%
! TGTATTATTAAGAATAACCC+AGG + chr3.4:83182761-83182780 None:intergenic 25.0%
! TTAATAGATAAGCTACTACA+AGG - chr3.4:83182281-83182300 MS.gene013631:CDS 25.0%
! TTGAAGTTGAAGTAACTATA+TGG - chr3.4:83183036-83183055 MS.gene013631:CDS 25.0%
! TTGTTCAAAAGTTGTTCATT+TGG + chr3.4:83183327-83183346 None:intergenic 25.0%
! TTTCAAATGGAACAAATGAT+TGG + chr3.4:83183398-83183417 None:intergenic 25.0%
!! ATATGTTTTGTCCATCTAAT+TGG + chr3.4:83182875-83182894 None:intergenic 25.0%
!! CAAATGTGGATATAATTTTC+TGG + chr3.4:83182382-83182401 None:intergenic 25.0%
!! CCATATTAACATGTCTTTTA+CGG + chr3.4:83182427-83182446 None:intergenic 25.0%
!!! AAACTTTTTTTATACGACCT+GGG - chr3.4:83182741-83182760 MS.gene013631:CDS 25.0%
!!! CAAGTTTTTTTTAATACCGT+AGG - chr3.4:83182664-83182683 MS.gene013631:CDS 25.0%
!!! TGAAATGGATTAGTTAATGT+TGG + chr3.4:83183246-83183265 None:intergenic 25.0%
AATTATATACCTTGGATGCA+CGG + chr3.4:83182984-83183003 None:intergenic 30.0%
ATAATCTCACTTGCCATATT+TGG + chr3.4:83183531-83183550 None:intergenic 30.0%
CAAATTTACATACCTCTTTC+TGG + chr3.4:83182582-83182601 None:intergenic 30.0%
CCGTAAAAGACATGTTAATA+TGG - chr3.4:83182424-83182443 MS.gene013631:CDS 30.0%
TAATCTCACTTGCCATATTT+GGG + chr3.4:83183530-83183549 None:intergenic 30.0%
TTCAAAAGTTGTTCATTTGG+TGG + chr3.4:83183324-83183343 None:intergenic 30.0%
! TCAAGTACATGGTTTTCAAA+TGG + chr3.4:83183411-83183430 None:intergenic 30.0%
! TTTGTTTCTTGGGTTTGAAA+TGG + chr3.4:83183261-83183280 None:intergenic 30.0%
!! ATGATTGGTTTAGGAAATCT+AGG + chr3.4:83183383-83183402 None:intergenic 30.0%
!! ATGGAACAAATGATTGGTTT+AGG + chr3.4:83183392-83183411 None:intergenic 30.0%
!! ATTTGAAAAGGCACTTGAAA+TGG - chr3.4:83182397-83182416 MS.gene013631:CDS 30.0%
!!! GAAACTTTTTTTATACGACC+TGG - chr3.4:83182740-83182759 MS.gene013631:CDS 30.0%
AAGAGAAAAATCGTGAGTTG+TGG + chr3.4:83183644-83183663 None:intergenic 35.0%
AATCTCACGTGTTTCATTAG+AGG + chr3.4:83182354-83182373 None:intergenic 35.0%
ACTTATTCCTGAATTTGCAG+GGG + chr3.4:83183475-83183494 None:intergenic 35.0%
AGATAAGCTACTACAAGGAT+TGG - chr3.4:83182286-83182305 MS.gene013631:CDS 35.0%
ATCACGGTTAAAATCTCTGT+CGG + chr3.4:83182549-83182568 None:intergenic 35.0%
GACTTATTCCTGAATTTGCA+GGG + chr3.4:83183476-83183495 None:intergenic 35.0%
GGGTATAGTTGGAGAAAATA+TGG - chr3.4:83182938-83182957 MS.gene013631:CDS 35.0%
TGACTTATTCCTGAATTTGC+AGG + chr3.4:83183477-83183496 None:intergenic 35.0%
! AGTTGTGGAATGTGAAGTTT+TGG + chr3.4:83183629-83183648 None:intergenic 35.0%
! CAAAGCTACTTTCAAGTACA+TGG + chr3.4:83183422-83183441 None:intergenic 35.0%
! TTCAAGTGCCTTTTCAAATG+TGG + chr3.4:83182396-83182415 None:intergenic 35.0%
! TTTGTTGATCAAGGCTTTGA+GGG + chr3.4:83183299-83183318 None:intergenic 35.0%
! TTTTGTTGATCAAGGCTTTG+AGG + chr3.4:83183300-83183319 None:intergenic 35.0%
!! AAACATATTCGAGTTGAACC+AGG - chr3.4:83182887-83182906 MS.gene013631:intron 35.0%
!! ATCTTTGAAATCACATACCG+AGG - chr3.4:83183183-83183202 MS.gene013631:intron 35.0%
!! TTGGTGGTTTTTGTTGATCA+AGG + chr3.4:83183308-83183327 None:intergenic 35.0%
AAACACACCAACTGCTTCAA+TGG - chr3.4:83183572-83183591 MS.gene013631:CDS 40.0%
AAGGTGGCGAATCTGATATT+CGG + chr3.4:83182499-83182518 None:intergenic 40.0%
AATATGGCGAGTGATCTGAA+TGG - chr3.4:83182699-83182718 MS.gene013631:CDS 40.0%
ACAATTGCTTCCAATGTGGT+TGG - chr3.4:83183219-83183238 MS.gene013631:intron 40.0%
ATATGGCGAGTGATCTGAAT+GGG - chr3.4:83182700-83182719 MS.gene013631:CDS 40.0%
ATCACAATGCTTCCAGAAAG+AGG - chr3.4:83182567-83182586 MS.gene013631:CDS 40.0%
ATGCACAATTGCTTCCAATG+TGG - chr3.4:83183215-83183234 MS.gene013631:intron 40.0%
CAACTATACCCATCATCAAG+AGG + chr3.4:83182929-83182948 None:intergenic 40.0%
GCACTCATAGAAACGTTCTA+GGG - chr3.4:83183115-83183134 MS.gene013631:intron 40.0%
GGAACACTCTACCAATTAGA+TGG - chr3.4:83182861-83182880 MS.gene013631:intron 40.0%
TAATGTTGGACCAACCACAT+TGG + chr3.4:83183232-83183251 None:intergenic 40.0%
TAGGGTGCGCATAGATAATA+TGG - chr3.4:83182683-83182702 MS.gene013631:CDS 40.0%
TATGGTCAGAAAGACATCCT+TGG - chr3.4:83182956-83182975 MS.gene013631:CDS 40.0%
TATGTTTCCCCTGCAAATTC+AGG - chr3.4:83183465-83183484 MS.gene013631:CDS 40.0%
TATTATCTATGCGCACCCTA+CGG + chr3.4:83182683-83182702 None:intergenic 40.0%
TCCAAGTGTGTTCCCAAATA+TGG - chr3.4:83183515-83183534 MS.gene013631:CDS 40.0%
TGCACTCATAGAAACGTTCT+AGG - chr3.4:83183114-83183133 MS.gene013631:intron 40.0%
TTGTCGAGAAAACTCTCGTT+AGG - chr3.4:83182635-83182654 MS.gene013631:CDS 40.0%
TTTGCAGGGGAAACATATGA+AGG + chr3.4:83183462-83183481 None:intergenic 40.0%
! CTTTTGAACAACCTACGAAC+AGG - chr3.4:83183336-83183355 MS.gene013631:intron 40.0%
!! CTCTTGATGATGGGTATAGT+TGG - chr3.4:83182927-83182946 MS.gene013631:intron 40.0%
!! TAGAAACGTTCTAGGGTGTT+TGG - chr3.4:83183122-83183141 MS.gene013631:intron 40.0%
!! TATTCGAGTTGAACCAGGAA+TGG - chr3.4:83182892-83182911 MS.gene013631:intron 40.0%
!! TTCTGGAAGCATTGTGATCA+CGG + chr3.4:83182565-83182584 None:intergenic 40.0%
GAACTCTTAGACCTGTTCGT+AGG + chr3.4:83183350-83183369 None:intergenic 45.0%
GCCATATTTGGGAACACACT+TGG + chr3.4:83183519-83183538 None:intergenic 45.0%
GCTACTACAAGGATTGGAGT+TGG - chr3.4:83182292-83182311 MS.gene013631:CDS 45.0%
GGAAACTCCATTGAAGCAGT+TGG + chr3.4:83183582-83183601 None:intergenic 45.0%
TGGAAGCAATTGTGCATGTG+TGG + chr3.4:83183212-83183231 None:intergenic 45.0%
TGGCGAATCTGATATTCGGA+TGG + chr3.4:83182495-83182514 None:intergenic 45.0%
TTGTGCATGTGTGGTTTCCT+CGG + chr3.4:83183203-83183222 None:intergenic 45.0%
TTTGGGAACACACTTGGAGT+TGG + chr3.4:83183513-83183532 None:intergenic 45.0%
! TGGAAGGTCCTCTTGATGAT+GGG - chr3.4:83182918-83182937 MS.gene013631:intron 45.0%
!! AAAAAATAAATATAAAAACT+AGG + chr3.4:83182838-83182857 None:intergenic 5.0%
!! AAAAATAAATATAAAAACTA+GGG + chr3.4:83182837-83182856 None:intergenic 5.0%
AGTTGAACCAGGAATGGCAG+TGG - chr3.4:83182898-83182917 MS.gene013631:intron 50.0%
CAACAATACCCTTCAGCCTC+AGG - chr3.4:83182461-83182480 MS.gene013631:CDS 50.0%
CAAGTGTTCCTGAGGCTGAA+GGG + chr3.4:83182472-83182491 None:intergenic 50.0%
GTGGAAGGTCCTCTTGATGA+TGG - chr3.4:83182917-83182936 MS.gene013631:intron 50.0%
GTGTGTGTGTGTGTATGCAG+AGG - chr3.4:83183081-83183100 MS.gene013631:intron 50.0%
GTGTTTGCAGCAGAAGTAGC+TGG + chr3.4:83183558-83183577 None:intergenic 50.0%
TTTGCAGCAGAAGTAGCTGG+TGG + chr3.4:83183555-83183574 None:intergenic 50.0%
!! CTGAGGCTGAAGGGTATTGT+TGG + chr3.4:83182463-83182482 None:intergenic 50.0%
CTGTCGGAGTCTTCACTTCG+CGG + chr3.4:83182533-83182552 None:intergenic 55.0%
GAACCAGGAATGGCAGTGGA+AGG - chr3.4:83182902-83182921 MS.gene013631:intron 55.0%
GCAAGTGTTCCTGAGGCTGA+AGG + chr3.4:83182473-83182492 None:intergenic 55.0%
TCGGATGGCAAGTGTTCCTG+AGG + chr3.4:83182480-83182499 None:intergenic 55.0%
! CTTCGCGGACTACAGCTCAA+AGG + chr3.4:83182518-83182537 None:intergenic 55.0%
ACCTTGGATGCACGGCTCCA+AGG + chr3.4:83182976-83182995 None:intergenic 60.0%
GGACCTTCCACTGCCATTCC+TGG + chr3.4:83182908-83182927 None:intergenic 60.0%
TCCTTGGAGCCGTGCATCCA+AGG - chr3.4:83182972-83182991 MS.gene013631:CDS 60.0%
! CGCGGACTACAGCTCAAAGG+TGG + chr3.4:83182515-83182534 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 83182263 83183665 83182263 ID=MS.gene013631
chr3.4 mRNA 83182263 83183665 83182263 ID=MS.gene013631.t1;Parent=MS.gene013631
chr3.4 exon 83183340 83183665 83183340 ID=MS.gene013631.t1.exon1;Parent=MS.gene013631.t1
chr3.4 CDS 83183340 83183665 83183340 ID=cds.MS.gene013631.t1;Parent=MS.gene013631.t1
chr3.4 exon 83182935 83183066 83182935 ID=MS.gene013631.t1.exon2;Parent=MS.gene013631.t1
chr3.4 CDS 83182935 83183066 83182935 ID=cds.MS.gene013631.t1;Parent=MS.gene013631.t1
chr3.4 exon 83182263 83182815 83182263 ID=MS.gene013631.t1.exon3;Parent=MS.gene013631.t1
chr3.4 CDS 83182263 83182815 83182263 ID=cds.MS.gene013631.t1;Parent=MS.gene013631.t1
Gene Sequence

>MS.gene013631

ATGGGAGATTCAAAGAACTTAATAGATAAGCTACTACAAGGATTGGAGTTGGCAAGACAGCTTCAAATCTATCTCAACGTGTCATCTTCCTCTAATGAAACACGTGAGATTTTGATCCAGAAAATTATATCCACATTTGAAAAGGCACTTGAAATGGTTAACCGTAAAAGACATGTTAATATGGGTGAATCTTCACACCAACAATACCCTTCAGCCTCAGGAACACTTGCCATCCGAATATCAGATTCGCCACCTTTGAGCTGTAGTCCGCGAAGTGAAGACTCCGACAGAGATTTTAACCGTGATCACAATGCTTCCAGAAAGAGGTATGTAAATTTGTTAACTACTCTACTATTAATTAGACACATTAGATTGTCGAGAAAACTCTCGTTAGGCATTATCAAGTTTTTTTTAATACCGTAGGGTGCGCATAGATAATATGGCGAGTGATCTGAATGGGAGAAGTGTAGCTCTTTAGAAACTTTTTTTATACGACCTGGGTTATTCTTAATAATACAAAAGTTGCTTGATTTGCTTCTCTATATGTATTGTACTAAACATGTGACCTTTACCCTAGTTTTTATATTTATTTTTTTTAGGAACACTCTACCAATTAGATGGACAAAACATATTCGAGTTGAACCAGGAATGGCAGTGGAAGGTCCTCTTGATGATGGGTATAGTTGGAGAAAATATGGTCAGAAAGACATCCTTGGAGCCGTGCATCCAAGGTATATAATTTCTCAATTTAATGTTGCATTTCTATTGATAGATTGAAGTTGAAGTAACTATATGGTAATATTAATTTGTGTGTATGTGTGTGTGTGTGTGTATGCAGAGGCTATTACAGATGCACTCATAGAAACGTTCTAGGGTGTTTGGCAACAAAGCAAGTACAAAGATCTGATGAAGACCCAACTATCTTTGAAATCACATACCGAGGAAACCACACATGCACAATTGCTTCCAATGTGGTTGGTCCAACATTAACTAATCCATTTCAAACCCAAGAAACAAATATTGTTAACACAAACCCTCAAAGCCTTGATCAACAAAAACCACCAAATGAACAACTTTTGAACAACCTACGAACAGGTCTAAGAGTTCAAACTGAGAACCTAGATTTCCTAAACCAATCATTTGTTCCATTTGAAAACCATGTACTTGAAAGTAGCTTTGCTGAAAATTTCAATTCTCCTTCATATGTTTCCCCTGCAAATTCAGGAATAAGTCACTTCTCAATGTCTCCAACTCCAAGTGTGTTCCCAAATATGGCAAGTGAGATTATTCCACCAGCTACTTCTGCTGCAAACACACCAACTGCTTCAATGGAGTTTCCATTTGATCAATTTGAATTTGATGGCCAAAACTTCACATTCCACAACTCACGATTTTTCTCTTAA
Protein sequence

>MS.gene013631.t1

MGDSKNLIDKLLQGLELARQLQIYLNVSSSSNETREILIQKIISTFEKALEMVNRKRHVNMGESSHQQYPSASGTLAIRISDSPPLSCSPRSEDSDRDFNRDHNASRKRNTLPIRWTKHIRVEPGMAVEGPLDDGYSWRKYGQKDILGAVHPRCTHRNVLGCLATKQVQRSDEDPTIFEITYRGNHTCTIASNVVGPTLTNPFQTQETNIVNTNPQSLDQQKPPNEQLLNNLRTGLRVQTENLDFLNQSFVPFENHVLESSFAENFNSPSYVSPANSGISHFSMSPTPSVFPNMASEIIPPATSAANTPTASMEFPFDQFEFDGQNFTFHNSRFFS