AlfalfaGEDB | MS.gene013746

M. sativa cultivar XinJiangDaYe / MS.gene013746

KEGG: K10523
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00651.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013746.t1	XP_013461323.1	100	128	0	0	1	128	117	244	1.10E-64	255.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013746.t1	Q6DBN1	71.1	128	37	0	1	128	102	229	1.8e-47	189.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013746.t1	I3T836	100.0	128	0	0	1	128	117	244	7.9e-65	255.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene013746.t1	TR	TRAF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013746.t1	MTR_3g088660	100.000	128	0	0	1	128	117	244	2.36e-91	265

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene013746.t1	AT4G08455	71.094	128	37	0	1	128	129	256	1.50e-65	200
MS.gene013746.t1	AT4G37610	32.283	127	79	3	2	123	90	214	9.07e-11	58.2

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTAAAACTAATAGGTTAC+AGG	0.263882	3.4:+81686773	None:intergenic
ATTTGATCTCCAAACTGAAT+TGG	0.305803	3.4:-81686707	MS.gene013746:CDS
GGCAGTGATCATGGAGAATA+TGG	0.334507	3.4:-81686616	MS.gene013746:CDS
CTAATAGGTTACAGGCCATT+TGG	0.355315	3.4:+81686781	None:intergenic
AGCTTTCTCCCAATTCAGTT+TGG	0.363821	3.4:+81686698	None:intergenic
TGAAGCTTATCTTGCCAAAC+AGG	0.369315	3.4:-81686523	None:intergenic
CCAATTGCAAGATGCAGCTT+TGG	0.375309	3.4:-81686637	MS.gene013746:CDS
GGCTGCAGTATTAACCTGTT+TGG	0.400932	3.4:+81686509	None:intergenic
TTTGATCTCCAAACTGAATT+GGG	0.405372	3.4:-81686706	MS.gene013746:CDS
CCAAAGCTGCATCTTGCAAT+TGG	0.405862	3.4:+81686637	None:intergenic
TACAGGCCATTTGGTTATCA+AGG	0.418138	3.4:+81686790	None:intergenic
CGAAACATCAGCAATCTTGA+TGG	0.418642	3.4:+81686863	None:intergenic
TGCAGCTTTGGCAGTGATCA+TGG	0.425358	3.4:-81686625	MS.gene013746:CDS
AGCTGCATCTTGCAATTGGT+TGG	0.493566	3.4:+81686641	None:intergenic
AACTAATCCTCGTTTAGTTG+TGG	0.493649	3.4:-81686553	MS.gene013746:CDS
GTATCAGGTGAAGCATCTTA+AGG	0.500142	3.4:-81686745	MS.gene013746:CDS
TGAAGATTACACGGAGTTGG+TGG	0.522112	3.4:-81686577	MS.gene013746:CDS
AAATGAAGATTACACGGAGT+TGG	0.527013	3.4:-81686580	MS.gene013746:CDS
AGCATGCCTTGATAACCAAA+TGG	0.551457	3.4:-81686796	MS.gene013746:CDS
AGAATGATATGGAGGAAAGC+CGG	0.570838	3.4:-81686893	None:intergenic
GGCATTGTGTTGATGTGCAA+AGG	0.583175	3.4:+81686662	None:intergenic
CAATCTTGATGGTGCCACTC+CGG	0.589211	3.4:+81686874	None:intergenic
TATATTTCCACAACTAAACG+AGG	0.674137	3.4:+81686546	None:intergenic
GATATGGAGGAAAGCCGGAG+TGG	0.686483	3.4:-81686888	MS.gene013746:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CACTAAAAATGAAGATTACA+CGG	-	chr3.4:81686823-81686842	MS.gene013746:CDS	25.0%
!!	ACTTTTCTCCTAAAACTAAT+AGG	+	chr3.4:81686646-81686665	None:intergenic	25.0%
!!!	TGTAATCTTCATTTTTAGTG+AGG	+	chr3.4:81686823-81686842	None:intergenic	25.0%
	ATTTGATCTCCAAACTGAAT+TGG	-	chr3.4:81686702-81686721	MS.gene013746:CDS	30.0%
	TATATTTCCACAACTAAACG+AGG	+	chr3.4:81686866-81686885	None:intergenic	30.0%
	TCCTAAAACTAATAGGTTAC+AGG	+	chr3.4:81686639-81686658	None:intergenic	30.0%
	TTTGATCTCCAAACTGAATT+GGG	-	chr3.4:81686703-81686722	MS.gene013746:CDS	30.0%
!!!	AGTTTTAGGAGAAAAGTATC+AGG	-	chr3.4:81686649-81686668	MS.gene013746:CDS	30.0%
	AAATGAAGATTACACGGAGT+TGG	-	chr3.4:81686829-81686848	MS.gene013746:CDS	35.0%
	AACTAATCCTCGTTTAGTTG+TGG	-	chr3.4:81686856-81686875	MS.gene013746:CDS	35.0%
!!!	GCCTGTAACCTATTAGTTTT+AGG	-	chr3.4:81686635-81686654	MS.gene013746:CDS	35.0%
	AGCTTTCTCCCAATTCAGTT+TGG	+	chr3.4:81686714-81686733	None:intergenic	40.0%
	CGAAACATCAGCAATCTTGA+TGG	+	chr3.4:81686549-81686568	None:intergenic	40.0%
	CTAATAGGTTACAGGCCATT+TGG	+	chr3.4:81686631-81686650	None:intergenic	40.0%
	TACAGGCCATTTGGTTATCA+AGG	+	chr3.4:81686622-81686641	None:intergenic	40.0%
!	AGCATGCCTTGATAACCAAA+TGG	-	chr3.4:81686613-81686632	MS.gene013746:CDS	40.0%
!	GTATCAGGTGAAGCATCTTA+AGG	-	chr3.4:81686664-81686683	MS.gene013746:CDS	40.0%
	AGCTGCATCTTGCAATTGGT+TGG	+	chr3.4:81686771-81686790	None:intergenic	45.0%
	CCAAAGCTGCATCTTGCAAT+TGG	+	chr3.4:81686775-81686794	None:intergenic	45.0%
	CCAATTGCAAGATGCAGCTT+TGG	-	chr3.4:81686772-81686791	MS.gene013746:CDS	45.0%
	GGCAGTGATCATGGAGAATA+TGG	-	chr3.4:81686793-81686812	MS.gene013746:CDS	45.0%
	TGAAGATTACACGGAGTTGG+TGG	-	chr3.4:81686832-81686851	MS.gene013746:CDS	45.0%
!	GGCATTGTGTTGATGTGCAA+AGG	+	chr3.4:81686750-81686769	None:intergenic	45.0%
!	TGCAGCTTTGGCAGTGATCA+TGG	-	chr3.4:81686784-81686803	MS.gene013746:CDS	50.0%
!!	CAATCTTGATGGTGCCACTC+CGG	+	chr3.4:81686538-81686557	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	81686524	81686907	81686524	ID=MS.gene013746
chr3.4	mRNA	81686524	81686907	81686524	ID=MS.gene013746.t1;Parent=MS.gene013746
chr3.4	exon	81686524	81686907	81686524	ID=MS.gene013746.t1.exon1;Parent=MS.gene013746.t1
chr3.4	CDS	81686524	81686907	81686524	ID=cds.MS.gene013746.t1;Parent=MS.gene013746.t1

Gene Sequence

>MS.gene013746

ATGGAGGAAAGCCGGAGTGGCACCATCAAGATTGCTGATGTTTCGTACGATGCTCTTCGTGCTTTTGTTAACTACTTGTACACTGCTGAAGCATGCCTTGATAACCAAATGGCCTGTAACCTATTAGTTTTAGGAGAAAAGTATCAGGTGAAGCATCTTAAGGCGTATTGTGAGAAGTATTTGATCTCCAAACTGAATTGGGAGAAAGCTATTGTCAACTTTGCCTTTGCACATCAACACAATGCCAACCAATTGCAAGATGCAGCTTTGGCAGTGATCATGGAGAATATGGACAACCTCACTAAAAATGAAGATTACACGGAGTTGGTGGAAACTAATCCTCGTTTAGTTGTGGAAATATATGAAGCTTATCTTGCCAAACAG

Protein sequence

>MS.gene013746.t1

MEESRSGTIKIADVSYDALRAFVNYLYTAEACLDNQMACNLLVLGEKYQVKHLKAYCEKYLISKLNWEKAIVNFAFAHQHNANQLQDAALAVIMENMDNLTKNEDYTELVETNPRLVVEIYEAYLAKQ