Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013747.t1 | XP_013461323.1 | 100 | 128 | 0 | 0 | 1 | 128 | 117 | 244 | 1.10E-64 | 255.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013747.t1 | Q6DBN1 | 71.1 | 128 | 37 | 0 | 1 | 128 | 102 | 229 | 1.8e-47 | 189.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013747.t1 | I3T836 | 100.0 | 128 | 0 | 0 | 1 | 128 | 117 | 244 | 7.9e-65 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene013747.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013747.t1 | MTR_3g088660 | 100.000 | 128 | 0 | 0 | 1 | 128 | 117 | 244 | 2.36e-91 | 265 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013747.t1 | AT4G08455 | 71.094 | 128 | 37 | 0 | 1 | 128 | 129 | 256 | 1.50e-65 | 200 |
| MS.gene013747.t1 | AT4G37610 | 32.283 | 127 | 79 | 3 | 2 | 123 | 90 | 214 | 9.07e-11 | 58.2 |
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAAAACTAATAGGTTAC+AGG | 0.263882 | 3.3:-74515670 | None:intergenic |
| ATTTGATCTCCAAACTGAAT+TGG | 0.305803 | 3.3:+74515736 | MS.gene013747:CDS |
| GGCAGTGATCATGGAGAATA+TGG | 0.334507 | 3.3:+74515827 | MS.gene013747:CDS |
| CTAATAGGTTACAGGCCATT+TGG | 0.355315 | 3.3:-74515662 | None:intergenic |
| AGCTTTCTCCCAATTCAGTT+TGG | 0.363821 | 3.3:-74515745 | None:intergenic |
| TGAAGCTTATCTTGCCAAAC+AGG | 0.369315 | 3.3:+74515920 | MS.gene013747:CDS |
| CCAATTGCAAGATGCAGCTT+TGG | 0.375309 | 3.3:+74515806 | MS.gene013747:CDS |
| GGCTGCAGTATTAACCTGTT+TGG | 0.400932 | 3.3:-74515934 | None:intergenic |
| TTTGATCTCCAAACTGAATT+GGG | 0.405372 | 3.3:+74515737 | MS.gene013747:CDS |
| CCAAAGCTGCATCTTGCAAT+TGG | 0.405862 | 3.3:-74515806 | None:intergenic |
| TACAGGCCATTTGGTTATCA+AGG | 0.418138 | 3.3:-74515653 | None:intergenic |
| CGAAACATCAGCAATCTTGA+TGG | 0.418642 | 3.3:-74515580 | None:intergenic |
| TGCAGCTTTGGCAGTGATCA+TGG | 0.425358 | 3.3:+74515818 | MS.gene013747:CDS |
| AGCTGCATCTTGCAATTGGT+TGG | 0.493566 | 3.3:-74515802 | None:intergenic |
| AACTAATCCTCGTTTAGTTG+TGG | 0.493649 | 3.3:+74515890 | MS.gene013747:CDS |
| GTATCAGGTGAAGCATCTTA+AGG | 0.500142 | 3.3:+74515698 | MS.gene013747:CDS |
| TGAAGATTACACGGAGTTGG+TGG | 0.522112 | 3.3:+74515866 | MS.gene013747:CDS |
| AAATGAAGATTACACGGAGT+TGG | 0.527013 | 3.3:+74515863 | MS.gene013747:CDS |
| AGCATGCCTTGATAACCAAA+TGG | 0.551457 | 3.3:+74515647 | MS.gene013747:CDS |
| AGAATGATATGGAGGAAAGC+CGG | 0.570838 | 3.3:+74515550 | None:intergenic |
| GGCATTGTGTTGATGTGCAA+AGG | 0.583175 | 3.3:-74515781 | None:intergenic |
| CAATCTTGATGGTGCCACTC+CGG | 0.589211 | 3.3:-74515569 | None:intergenic |
| TATATTTCCACAACTAAACG+AGG | 0.674137 | 3.3:-74515897 | None:intergenic |
| GATATGGAGGAAAGCCGGAG+TGG | 0.686483 | 3.3:+74515555 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CACTAAAAATGAAGATTACA+CGG | + | chr3.3:74515857-74515876 | MS.gene013747:CDS | 25.0% |
| !! | ACTTTTCTCCTAAAACTAAT+AGG | - | chr3.3:74515680-74515699 | None:intergenic | 25.0% |
| !!! | TGTAATCTTCATTTTTAGTG+AGG | - | chr3.3:74515857-74515876 | None:intergenic | 25.0% |
| ATTTGATCTCCAAACTGAAT+TGG | + | chr3.3:74515736-74515755 | MS.gene013747:CDS | 30.0% | |
| TATATTTCCACAACTAAACG+AGG | - | chr3.3:74515900-74515919 | None:intergenic | 30.0% | |
| TCCTAAAACTAATAGGTTAC+AGG | - | chr3.3:74515673-74515692 | None:intergenic | 30.0% | |
| TTTGATCTCCAAACTGAATT+GGG | + | chr3.3:74515737-74515756 | MS.gene013747:CDS | 30.0% | |
| !!! | AGTTTTAGGAGAAAAGTATC+AGG | + | chr3.3:74515683-74515702 | MS.gene013747:CDS | 30.0% |
| AAATGAAGATTACACGGAGT+TGG | + | chr3.3:74515863-74515882 | MS.gene013747:CDS | 35.0% | |
| AACTAATCCTCGTTTAGTTG+TGG | + | chr3.3:74515890-74515909 | MS.gene013747:CDS | 35.0% | |
| !!! | GCCTGTAACCTATTAGTTTT+AGG | + | chr3.3:74515669-74515688 | MS.gene013747:CDS | 35.0% |
| AGCTTTCTCCCAATTCAGTT+TGG | - | chr3.3:74515748-74515767 | None:intergenic | 40.0% | |
| CGAAACATCAGCAATCTTGA+TGG | - | chr3.3:74515583-74515602 | None:intergenic | 40.0% | |
| CTAATAGGTTACAGGCCATT+TGG | - | chr3.3:74515665-74515684 | None:intergenic | 40.0% | |
| TACAGGCCATTTGGTTATCA+AGG | - | chr3.3:74515656-74515675 | None:intergenic | 40.0% | |
| ! | AGCATGCCTTGATAACCAAA+TGG | + | chr3.3:74515647-74515666 | MS.gene013747:CDS | 40.0% |
| ! | GTATCAGGTGAAGCATCTTA+AGG | + | chr3.3:74515698-74515717 | MS.gene013747:CDS | 40.0% |
| AGCTGCATCTTGCAATTGGT+TGG | - | chr3.3:74515805-74515824 | None:intergenic | 45.0% | |
| CCAAAGCTGCATCTTGCAAT+TGG | - | chr3.3:74515809-74515828 | None:intergenic | 45.0% | |
| CCAATTGCAAGATGCAGCTT+TGG | + | chr3.3:74515806-74515825 | MS.gene013747:CDS | 45.0% | |
| GGCAGTGATCATGGAGAATA+TGG | + | chr3.3:74515827-74515846 | MS.gene013747:CDS | 45.0% | |
| TGAAGATTACACGGAGTTGG+TGG | + | chr3.3:74515866-74515885 | MS.gene013747:CDS | 45.0% | |
| ! | GGCATTGTGTTGATGTGCAA+AGG | - | chr3.3:74515784-74515803 | None:intergenic | 45.0% |
| ! | TGCAGCTTTGGCAGTGATCA+TGG | + | chr3.3:74515818-74515837 | MS.gene013747:CDS | 50.0% |
| !! | CAATCTTGATGGTGCCACTC+CGG | - | chr3.3:74515572-74515591 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 74515558 | 74515941 | 74515558 | ID=MS.gene013747 |
| chr3.3 | mRNA | 74515558 | 74515941 | 74515558 | ID=MS.gene013747.t1;Parent=MS.gene013747 |
| chr3.3 | exon | 74515558 | 74515941 | 74515558 | ID=MS.gene013747.t1.exon1;Parent=MS.gene013747.t1 |
| chr3.3 | CDS | 74515558 | 74515941 | 74515558 | ID=cds.MS.gene013747.t1;Parent=MS.gene013747.t1 |
| Gene Sequence |
| Protein sequence |
