Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013774.t1 | KEH35336.1 | 66.5 | 236 | 19 | 2 | 2 | 177 | 13 | 248 | 4.00E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013774.t1 | Q8LAV5 | 27.5 | 102 | 72 | 1 | 1 | 102 | 1 | 100 | 2.1e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013774.t1 | A0A072V0X2 | 66.5 | 236 | 19 | 2 | 2 | 177 | 13 | 248 | 2.9e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene013774.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013774.t1 | MTR_3g088515 | 66.525 | 236 | 19 | 2 | 2 | 177 | 13 | 248 | 5.85e-97 | 281 |
| MS.gene013774.t1 | MTR_1g046200 | 49.321 | 221 | 60 | 3 | 5 | 176 | 2 | 219 | 1.11e-58 | 186 |
| MS.gene013774.t1 | MTR_1g046340 | 80.180 | 111 | 22 | 0 | 1 | 111 | 1 | 111 | 1.46e-58 | 186 |
| MS.gene013774.t1 | MTR_1g046340 | 88.095 | 42 | 3 | 1 | 137 | 176 | 189 | 230 | 1.57e-18 | 81.6 |
| MS.gene013774.t1 | MTR_1g046320 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 2.24e-47 | 157 |
| MS.gene013774.t1 | MTR_1g046320 | 76.744 | 43 | 8 | 1 | 136 | 176 | 187 | 229 | 1.35e-15 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTCATCATCTTCAAGTTT+TGG | 0.269280 | 3.4:+81634925 | None:intergenic |
| GCATAGAAGTATGGATTTCC+TGG | 0.326303 | 3.4:+81634880 | None:intergenic |
| CAGAGAAGAAAATTCATTAG+AGG | 0.353395 | 3.4:-81635449 | MS.gene013774:CDS |
| ATGAGCTTCACAAAAGCAAT+TGG | 0.382357 | 3.4:+81635592 | None:intergenic |
| CGCGTCAAAGCACATTCAAT+TGG | 0.401347 | 3.4:-81635508 | MS.gene013774:CDS |
| ACATTACAGACCCAACGAAT+TGG | 0.430909 | 3.4:-81634854 | MS.gene013774:CDS |
| GATTGTGGTTTAGGGAGCAT+TGG | 0.466163 | 3.4:-81634964 | MS.gene013774:intron |
| AAGAACTAAACCAATTCGTT+GGG | 0.487807 | 3.4:+81634844 | None:intergenic |
| ATGAAAGCAGAAAAGAAGAA+AGG | 0.488071 | 3.4:-81635136 | MS.gene013774:intron |
| AAAGAACTAAACCAATTCGT+TGG | 0.488811 | 3.4:+81634843 | None:intergenic |
| CTCTTCCTCTATGATCCCTT+AGG | 0.492258 | 3.4:+81635536 | None:intergenic |
| TCTCATAATGCTTCTCTATG+AGG | 0.504327 | 3.4:+81635807 | None:intergenic |
| GGGATCATAGAGGAAGAGAT+TGG | 0.510517 | 3.4:-81635531 | MS.gene013774:CDS |
| AAGCACATTCAATTGGTGGC+AGG | 0.516729 | 3.4:-81635501 | MS.gene013774:CDS |
| AAGAAGAAAGGAGTTGACAA+GGG | 0.522584 | 3.4:-81635124 | MS.gene013774:CDS |
| AGAGAAGCATTATGAGAGAA+TGG | 0.527328 | 3.4:-81635801 | MS.gene013774:intron |
| GTTGAAATGTATATTCAGCC+AGG | 0.532204 | 3.4:-81634898 | MS.gene013774:CDS |
| AAAGAAGAAAGGAGTTGACA+AGG | 0.542225 | 3.4:-81635125 | MS.gene013774:CDS |
| TGAGCTTCACAAAAGCAATT+GGG | 0.545679 | 3.4:+81635593 | None:intergenic |
| GTCAAAGCACATTCAATTGG+TGG | 0.546694 | 3.4:-81635505 | MS.gene013774:CDS |
| TTTCAATATGTGTGAAAACA+AGG | 0.554136 | 3.4:+81635416 | None:intergenic |
| GAGGAACACCTTGGCAGTGT+TGG | 0.557953 | 3.4:+81635847 | None:intergenic |
| AGCTCATGCTTTCGAAACAA+AGG | 0.561664 | 3.4:-81635576 | MS.gene013774:CDS |
| GGAAGACTTTGAAGAAGTTG+AGG | 0.564880 | 3.4:+81635828 | None:intergenic |
| GCTCATGCTTTCGAAACAAA+GGG | 0.566004 | 3.4:-81635575 | MS.gene013774:CDS |
| GAAGAAGTTGAGGAACACCT+TGG | 0.598409 | 3.4:+81635838 | None:intergenic |
| TTTATCCTAAGGGATCATAG+AGG | 0.616153 | 3.4:-81635541 | MS.gene013774:CDS |
| AATTATGACCAACACTGCCA+AGG | 0.625272 | 3.4:-81635855 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCAGTTTTTTTTTTTTTTT+TGG | + | chr3.4:81635028-81635047 | None:intergenic | 10.0% |
| !! | AACTTAATAATAATAATCGG+AGG | + | chr3.4:81634950-81634969 | None:intergenic | 20.0% |
| !!! | ATTGCAATATTTTATCCTAA+GGG | - | chr3.4:81635149-81635168 | MS.gene013774:intron | 20.0% |
| !!! | TATTGCAATATTTTATCCTA+AGG | - | chr3.4:81635148-81635167 | MS.gene013774:intron | 20.0% |
| ! | ATCAATGTGTAAAAAGACAA+AGG | - | chr3.4:81635341-81635360 | MS.gene013774:intron | 25.0% |
| ! | ATTTACCTAATGATTGATTG+TGG | - | chr3.4:81635721-81635740 | MS.gene013774:intron | 25.0% |
| ! | GGAAAATAATAATATGTTGG+AGG | - | chr3.4:81635377-81635396 | MS.gene013774:CDS | 25.0% |
| ! | GGAGGAAAATAATAATATGT+TGG | - | chr3.4:81635374-81635393 | MS.gene013774:CDS | 25.0% |
| ! | TAATGATTGATTGTGGTTTA+GGG | - | chr3.4:81635728-81635747 | MS.gene013774:intron | 25.0% |
| ! | TTTCAATATGTGTGAAAACA+AGG | + | chr3.4:81635287-81635306 | None:intergenic | 25.0% |
| !! | TAATGTTTTGCATAGAAGTA+TGG | + | chr3.4:81635832-81635851 | None:intergenic | 25.0% |
| !!! | ATTTCATCATCTTCAAGTTT+TGG | + | chr3.4:81635778-81635797 | None:intergenic | 25.0% |
| ATGAAAGCAGAAAAGAAGAA+AGG | - | chr3.4:81635564-81635583 | MS.gene013774:CDS | 30.0% | |
| CAAAAACATACATGCTTTGA+AGG | + | chr3.4:81635637-81635656 | None:intergenic | 30.0% | |
| CAGAGAAGAAAATTCATTAG+AGG | - | chr3.4:81635251-81635270 | MS.gene013774:intron | 30.0% | |
| CTAAACCACAATCAATCATT+AGG | + | chr3.4:81635729-81635748 | None:intergenic | 30.0% | |
| CTAATGATTGATTGTGGTTT+AGG | - | chr3.4:81635727-81635746 | MS.gene013774:intron | 30.0% | |
| GTGTAAAAAGACAAAGGTAA+TGG | - | chr3.4:81635347-81635366 | MS.gene013774:intron | 30.0% | |
| TAATAATATGTTGGAGGATG+AGG | - | chr3.4:81635383-81635402 | MS.gene013774:CDS | 30.0% | |
| ! | AGATCAATGTTACTTAAGAG+TGG | + | chr3.4:81635055-81635074 | None:intergenic | 30.0% |
| AAAGAAGAAAGGAGTTGACA+AGG | - | chr3.4:81635575-81635594 | MS.gene013774:CDS | 35.0% | |
| AAGAAGAAAGGAGTTGACAA+GGG | - | chr3.4:81635576-81635595 | MS.gene013774:CDS | 35.0% | |
| ATGAGCTTCACAAAAGCAAT+TGG | + | chr3.4:81635111-81635130 | None:intergenic | 35.0% | |
| GTTGAAATGTATATTCAGCC+AGG | - | chr3.4:81635802-81635821 | MS.gene013774:CDS | 35.0% | |
| TCTCATAATGCTTCTCTATG+AGG | + | chr3.4:81634896-81634915 | None:intergenic | 35.0% | |
| TGAGCTTCACAAAAGCAATT+GGG | + | chr3.4:81635110-81635129 | None:intergenic | 35.0% | |
| TTTATCCTAAGGGATCATAG+AGG | - | chr3.4:81635159-81635178 | MS.gene013774:intron | 35.0% | |
| !! | AAAGACAAAGGTAATGGATG+AGG | - | chr3.4:81635353-81635372 | MS.gene013774:intron | 35.0% |
| !! | AGAGAAGCATTATGAGAGAA+TGG | - | chr3.4:81634899-81634918 | MS.gene013774:CDS | 35.0% |
| !!! | GGCTTTATTTTGATGATGGT+TGG | - | chr3.4:81635220-81635239 | MS.gene013774:intron | 35.0% |
| AATGGTAGAGGAAGAAGAAG+AGG | - | chr3.4:81635458-81635477 | MS.gene013774:CDS | 40.0% | |
| ACATTACAGACCCAACGAAT+TGG | - | chr3.4:81635846-81635865 | MS.gene013774:CDS | 40.0% | |
| AGCTCATGCTTTCGAAACAA+AGG | - | chr3.4:81635124-81635143 | MS.gene013774:CDS | 40.0% | |
| GAAGAAGATGAAGATGGTGA+TGG | - | chr3.4:81635480-81635499 | MS.gene013774:CDS | 40.0% | |
| GAAGAGGAAGAAGATGAAGA+TGG | - | chr3.4:81635474-81635493 | MS.gene013774:CDS | 40.0% | |
| GCTCATGCTTTCGAAACAAA+GGG | - | chr3.4:81635125-81635144 | MS.gene013774:CDS | 40.0% | |
| GTCAAAGCACATTCAATTGG+TGG | - | chr3.4:81635195-81635214 | MS.gene013774:intron | 40.0% | |
| ! | GCATAGAAGTATGGATTTCC+TGG | + | chr3.4:81635823-81635842 | None:intergenic | 40.0% |
| ! | GGAAGACTTTGAAGAAGTTG+AGG | + | chr3.4:81634875-81634894 | None:intergenic | 40.0% |
| ! | TGATGATGAAGACGATGATG+AGG | - | chr3.4:81635503-81635522 | MS.gene013774:CDS | 40.0% |
| !!! | GGCAGGCTTTATTTTGATGA+TGG | - | chr3.4:81635216-81635235 | MS.gene013774:intron | 40.0% |
| CGACGGTTATACAATGGTAG+AGG | - | chr3.4:81635446-81635465 | MS.gene013774:CDS | 45.0% | |
| CGCGTCAAAGCACATTCAAT+TGG | - | chr3.4:81635192-81635211 | MS.gene013774:intron | 45.0% | |
| CTCTTCCTCTATGATCCCTT+AGG | + | chr3.4:81635167-81635186 | None:intergenic | 45.0% | |
| GAAGAAGTTGAGGAACACCT+TGG | + | chr3.4:81634865-81634884 | None:intergenic | 45.0% | |
| GGGATCATAGAGGAAGAGAT+TGG | - | chr3.4:81635169-81635188 | MS.gene013774:intron | 45.0% | |
| TATGTTGGAGGATGAGGAAG+AGG | - | chr3.4:81635389-81635408 | MS.gene013774:CDS | 45.0% | |
| TGAAGACGATGATGAGGATG+AGG | - | chr3.4:81635509-81635528 | MS.gene013774:CDS | 45.0% | |
| TGACGACGACGGTTATACAA+TGG | - | chr3.4:81635440-81635459 | MS.gene013774:CDS | 45.0% | |
| ! | AAGCACATTCAATTGGTGGC+AGG | - | chr3.4:81635199-81635218 | MS.gene013774:intron | 45.0% |
| ! | GAAGAGGAAGGTGATGATGA+TGG | - | chr3.4:81635405-81635424 | MS.gene013774:CDS | 45.0% |
| ! | GATTGTGGTTTAGGGAGCAT+TGG | - | chr3.4:81635736-81635755 | MS.gene013774:intron | 45.0% |
| !! | GACAAAGGTAATGGATGAGG+AGG | - | chr3.4:81635356-81635375 | MS.gene013774:intron | 45.0% |
| !! | AAAAACTTAATAATAATAAT+CGG | + | chr3.4:81634953-81634972 | None:intergenic | 5.0% |
| CGATGATGAGGATGAGGATG+AGG | - | chr3.4:81635515-81635534 | MS.gene013774:CDS | 50.0% | |
| TTGGAGGATGAGGAAGAGGA+AGG | - | chr3.4:81635393-81635412 | MS.gene013774:CDS | 50.0% | |
| ! | GGAGATGATGATGACGACGA+CGG | - | chr3.4:81635429-81635448 | MS.gene013774:CDS | 50.0% |
| !! | GAGGAAGGTGATGATGATGG+TGG | - | chr3.4:81635408-81635427 | MS.gene013774:CDS | 50.0% |
| GAGGAACACCTTGGCAGTGT+TGG | + | chr3.4:81634856-81634875 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 81634849 | 81635873 | 81634849 | ID=MS.gene013774 |
| chr3.4 | mRNA | 81634849 | 81635873 | 81634849 | ID=MS.gene013774.t1;Parent=MS.gene013774 |
| chr3.4 | exon | 81635802 | 81635873 | 81635802 | ID=MS.gene013774.t1.exon1;Parent=MS.gene013774.t1 |
| chr3.4 | CDS | 81635802 | 81635873 | 81635802 | ID=cds.MS.gene013774.t1;Parent=MS.gene013774.t1 |
| chr3.4 | exon | 81635360 | 81635620 | 81635360 | ID=MS.gene013774.t1.exon2;Parent=MS.gene013774.t1 |
| chr3.4 | CDS | 81635360 | 81635620 | 81635360 | ID=cds.MS.gene013774.t1;Parent=MS.gene013774.t1 |
| chr3.4 | exon | 81635073 | 81635148 | 81635073 | ID=MS.gene013774.t1.exon3;Parent=MS.gene013774.t1 |
| chr3.4 | CDS | 81635073 | 81635148 | 81635073 | ID=cds.MS.gene013774.t1;Parent=MS.gene013774.t1 |
| chr3.4 | exon | 81634849 | 81634973 | 81634849 | ID=MS.gene013774.t1.exon4;Parent=MS.gene013774.t1 |
| chr3.4 | CDS | 81634849 | 81634973 | 81634849 | ID=cds.MS.gene013774.t1;Parent=MS.gene013774.t1 |
| Gene Sequence |
| Protein sequence |
