AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01386.t1 MTR_4g035820 42.308 442 208 8 21 459 35 432 5.25e-101 322
MS.gene01386.t1 MTR_6g014355 87.805 123 15 0 126 248 5 127 4.09e-67 219
MS.gene01386.t1 MTR_6g014355 67.114 149 39 3 363 511 107 245 7.41e-57 192
MS.gene01386.t1 MTR_5g017720 64.072 167 29 3 461 621 18 159 1.17e-62 206
MS.gene01386.t1 MTR_6g025700 93.506 77 5 0 1 77 1 77 1.07e-42 149
MS.gene01386.t1 MTR_8g447350 42.038 157 66 3 413 565 1 136 2.70e-29 115
MS.gene01386.t1 MTR_3g450850 36.318 201 114 4 65 256 50 245 2.73e-28 117
MS.gene01386.t1 MTR_2g035140 37.059 170 88 5 443 603 104 263 2.93e-23 100
MS.gene01386.t1 MTR_6g051425 25.874 286 191 8 46 322 1 274 2.94e-18 87.0
MS.gene01386.t1 MTR_2g098920 35.075 134 72 5 41 169 59 182 8.56e-15 75.5
MS.gene01386.t1 MTR_1g070355 31.405 121 77 3 68 186 80 196 1.67e-14 77.0
MS.gene01386.t1 MTR_1g061650 48.333 60 31 0 1 60 1 60 1.00e-13 67.0
MS.gene01386.t1 MTR_0402s0040 29.648 199 120 9 55 246 20 205 1.06e-13 74.3
MS.gene01386.t1 MTR_5g076070 30.857 175 109 5 21 187 6 176 6.47e-13 70.1
MS.gene01386.t1 MTR_2g098880 29.787 141 91 3 51 188 50 185 3.47e-12 67.4
MS.gene01386.t1 MTR_2g098880 29.787 141 91 3 51 188 62 197 3.71e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01386.t1 AT5G28530 32.163 541 310 10 35 523 17 552 1.43e-90 293
MS.gene01386.t1 AT5G28530 32.348 541 305 10 35 523 17 548 2.59e-90 293
MS.gene01386.t1 AT1G10240 38.290 269 156 3 260 523 338 601 8.06e-61 215
MS.gene01386.t1 AT1G10240 43.243 185 97 3 62 240 48 230 2.01e-38 151
MS.gene01386.t1 AT1G10240 38.290 269 156 3 260 523 338 601 8.06e-61 215
MS.gene01386.t1 AT1G10240 43.243 185 97 3 62 240 48 230 2.01e-38 151
MS.gene01386.t1 AT5G28530 33.459 266 172 3 260 523 353 615 1.15e-45 172
MS.gene01386.t1 AT5G28530 34.800 250 141 6 35 265 17 263 2.88e-35 142
MS.gene01386.t1 AT5G28530 33.835 266 167 3 260 523 353 611 2.08e-45 172
MS.gene01386.t1 AT5G28530 34.800 250 141 6 35 265 17 263 2.74e-35 142
MS.gene01386.t1 AT5G28530 33.835 266 167 3 260 523 353 611 2.08e-45 172
MS.gene01386.t1 AT5G28530 34.800 250 141 6 35 265 17 263 2.74e-35 142
MS.gene01386.t1 AT4G38180 29.670 273 185 3 261 527 361 632 5.68e-35 141
MS.gene01386.t1 AT4G38180 34.389 221 124 8 35 245 45 254 3.24e-22 102
MS.gene01386.t1 AT4G15090 29.197 274 186 5 260 527 336 607 3.52e-32 133
MS.gene01386.t1 AT4G15090 29.197 274 186 5 260 527 336 607 3.52e-32 133
MS.gene01386.t1 AT4G15090 29.197 274 186 5 260 527 336 607 3.52e-32 133
MS.gene01386.t1 AT4G15090 29.197 274 186 5 260 527 336 607 3.52e-32 133
MS.gene01386.t1 AT4G15090 29.197 274 186 5 260 527 336 607 3.52e-32 133
MS.gene01386.t1 AT1G76320 27.891 294 191 6 256 535 269 555 6.40e-31 129
MS.gene01386.t1 AT1G76320 27.891 294 191 6 256 535 269 555 6.97e-31 129
MS.gene01386.t1 AT1G76320 27.891 294 191 6 256 535 269 555 6.97e-31 129
MS.gene01386.t1 AT1G76320 27.891 294 191 6 256 535 269 555 1.02e-30 128
MS.gene01386.t1 AT4G38170 33.175 211 133 5 324 528 189 397 7.03e-29 121
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 282 563 1.96e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 282 563 1.96e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 225 506 2.07e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 296 577 2.15e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 282 563 2.17e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 282 563 2.17e-27 118
MS.gene01386.t1 AT4G19990 26.573 286 196 6 258 533 282 563 2.41e-27 118
MS.gene01386.t1 AT3G22170 26.619 278 196 5 260 531 345 620 6.74e-27 117
MS.gene01386.t1 AT3G22170 26.619 278 196 5 260 531 345 620 6.74e-27 117
MS.gene01386.t1 AT3G06250 28.866 291 187 7 258 537 434 715 4.28e-26 114
MS.gene01386.t1 AT3G06250 28.866 291 187 7 258 537 434 715 4.28e-26 114
MS.gene01386.t1 AT3G06250 28.866 291 187 7 258 537 434 715 4.28e-26 114
MS.gene01386.t1 AT2G27110 26.115 314 217 8 240 543 284 592 5.72e-25 110
MS.gene01386.t1 AT2G27110 26.115 314 217 8 240 543 284 592 5.72e-25 110
MS.gene01386.t1 AT2G27110 26.115 314 217 8 240 543 139 447 6.34e-25 110
MS.gene01386.t1 AT5G18960 27.737 274 175 7 258 520 458 719 9.52e-24 107
MS.gene01386.t1 AT5G18960 27.737 274 175 7 258 520 458 719 9.52e-24 107
MS.gene01386.t1 AT1G52520 27.336 289 187 8 260 531 357 639 1.58e-21 99.8
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.32e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.87e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.87e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.99e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.99e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 281 547 5.99e-21 98.2
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 240 506 6.47e-21 97.8
MS.gene01386.t1 AT2G32250 24.254 268 197 4 261 523 240 506 7.14e-21 97.8
MS.gene01386.t1 AT1G80010 22.337 291 197 9 260 531 382 662 5.35e-13 72.8
MS.gene01386.t1 AT3G07500 35.417 144 80 4 28 169 4 136 7.99e-13 68.6
MS.gene01386.t1 AT3G59470 35.088 114 61 4 61 170 69 173 3.62e-12 67.4
MS.gene01386.t1 AT3G59470 35.088 114 61 4 61 170 69 173 3.62e-12 67.4
MS.gene01386.t1 AT3G59470 35.088 114 61 4 61 170 81 185 4.37e-12 67.4
MS.gene01386.t1 AT4G12850 38.532 109 56 3 61 167 10 109 6.96e-11 61.2
MS.gene01386.t1 AT4G12850 38.532 109 56 3 61 167 10 109 8.43e-11 60.8

Find 86 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACCATATGAGTATAAA+TGG 0.185807 2.2:+69536668 None:intergenic
TATCTTCGTGGACATTTCTT+TGG 0.192638 2.2:-69534814 MS.gene01386:CDS
ATCTTCGTGGACATTTCTTT+GGG 0.226407 2.2:-69534813 MS.gene01386:CDS
CACAAAAGCATAAAGTCTTA+TGG 0.233735 2.2:-69534379 MS.gene01386:CDS
GTTGCAAGCACTTTGAGTTT+TGG 0.246090 2.2:-69534334 MS.gene01386:CDS
TTTATGAGAATTATGCTAAA+AGG 0.251714 2.2:-69536047 MS.gene01386:CDS
ATATGCTTTCAAGAAGTTTC+AGG 0.261893 2.2:-69534486 MS.gene01386:CDS
TCGATCTTTATCCCCTTTAG+AGG 0.263472 2.2:-69534534 MS.gene01386:CDS
TCAATAACCTGCCACTCTTC+TGG 0.264829 2.2:+69535826 None:intergenic
GAAGCAAACAAGCACATAAA+AGG 0.309741 2.2:-69534871 MS.gene01386:CDS
GGAAGATCAGAGAGCATAAA+TGG 0.318954 2.2:-69534775 MS.gene01386:CDS
AGAAAACACTGCCCTATAAA+AGG 0.321677 2.2:+69536093 None:intergenic
TAGGTTGAGAATCACAAATT+TGG 0.324904 2.2:-69536226 MS.gene01386:CDS
CACATTACTGCTAAATTTAG+TGG 0.337411 2.2:-69535021 MS.gene01386:CDS
GTCTTGCCATTGAGGACATT+GGG 0.350598 2.2:-69534607 MS.gene01386:CDS
ATCTTACCGCAAAATTTCTA+AGG 0.354743 2.2:-69535746 MS.gene01386:CDS
TTACCACATGGTTCATTGTT+TGG 0.355616 2.2:+69536186 None:intergenic
TGTAAACTTGCTCCTCTAAA+GGG 0.366834 2.2:+69534522 None:intergenic
ACAAAAGCATAAAGTCTTAT+GGG 0.378373 2.2:-69534378 MS.gene01386:CDS
ACAACCCATTTATACTCATA+TGG 0.399798 2.2:-69536673 MS.gene01386:CDS
CAAGCAAATAATGCGTGTTA+TGG 0.401498 2.2:-69535677 MS.gene01386:CDS
GTTTGCTTCTAGATTGTACT+TGG 0.409330 2.2:+69534885 None:intergenic
ACAAAGTTGTAAAAGATCAT+TGG 0.414540 2.2:+69535555 None:intergenic
TGAACATGACTTTCTTTGTC+CGG 0.414585 2.2:+69534005 None:intergenic
GTATTCTCCACATTCTCAAT+AGG 0.415640 2.2:+69534170 None:intergenic
GAGAATTATGCTAAAAGGAG+TGG 0.417418 2.2:-69536042 MS.gene01386:CDS
AGCATCATATTCACTTCAAC+CGG 0.431672 2.2:+69534206 None:intergenic
TTGTAAACTTGCTCCTCTAA+AGG 0.433547 2.2:+69534521 None:intergenic
GGTCTTGCCATTGAGGACAT+TGG 0.445560 2.2:-69534608 MS.gene01386:CDS
AGTAATGATTGTCTTGTCTC+TGG 0.447168 2.2:+69535116 None:intergenic
CTATTTCGTTGTTCTTTAGA+CGG 0.448463 2.2:+69535916 None:intergenic
AGTTAAGCAACATTGGGTTC+CGG 0.456066 2.2:-69534839 MS.gene01386:CDS
CGTAATGGCAAGTAACTTGT+TGG 0.465122 2.2:+69534254 None:intergenic
TCATCACGACACCGACGTAA+TGG 0.465813 2.2:+69534239 None:intergenic
GTATGAAGTTAAGCAACATT+GGG 0.468238 2.2:-69534845 MS.gene01386:CDS
GCACAATGTTACAACTTGTC+CGG 0.470462 2.2:-69534024 MS.gene01386:CDS
TGGGTTCCGGCGTATCTTCG+TGG 0.482513 2.2:-69534826 MS.gene01386:CDS
TCTCAAAACAATCTTTATGT+AGG 0.484701 2.2:+69534279 None:intergenic
TGTATGAAGTTAAGCAACAT+TGG 0.488865 2.2:-69534846 MS.gene01386:CDS
CCCAAAACAAAACGATCAAA+AGG 0.494525 2.2:-69534101 MS.gene01386:CDS
CCGCCAAGGTCTAATACAAA+AGG 0.501842 2.2:-69534128 MS.gene01386:CDS
TTGCATCCACATCTCGAAGA+TGG 0.506363 2.2:+69535892 None:intergenic
ATACCATGTTGGATACATAC+AGG 0.511907 2.2:-69534568 MS.gene01386:CDS
TTAGGTTGGTCTTGCCATTG+AGG 0.515284 2.2:-69534615 MS.gene01386:intron
CCATGTGGTAATTCAGAAAG+TGG 0.515343 2.2:-69536174 MS.gene01386:CDS
ACAACCATATGAGTATAAAT+GGG 0.517525 2.2:+69536669 None:intergenic
AGCCAACAATCACCAAGAAA+AGG 0.519408 2.2:-69533983 MS.gene01386:CDS
ATTCATCAAGATAACATGAA+TGG 0.519717 2.2:-69535583 MS.gene01386:CDS
AGTGGGTTCTCAATTAGAAA+AGG 0.520009 2.2:-69536024 MS.gene01386:CDS
TTATGGGATGGTGATGTGGT+TGG 0.521321 2.2:-69534362 MS.gene01386:CDS
TTCGAAATAGATATTTCTCA+TGG 0.521497 2.2:-69534981 MS.gene01386:CDS
CTTCGTGGACATTTCTTTGG+GGG 0.522014 2.2:-69534811 MS.gene01386:CDS
TTACTGCTAAATTTAGTGGT+TGG 0.522241 2.2:-69535017 MS.gene01386:CDS
TATTTGCTTGACTGAAAGAT+CGG 0.528838 2.2:+69535690 None:intergenic
TCGTGATGAATTTCAAAGAC+CGG 0.536511 2.2:-69534225 MS.gene01386:CDS
AAAACGATCAAAAGGAGGAA+TGG 0.539810 2.2:-69534093 MS.gene01386:CDS
AATGATTGTCTTGTCTCTGG+AGG 0.545857 2.2:+69535119 None:intergenic
TTTCTAAGGAAGAAGAGAAG+TGG 0.552217 2.2:-69535732 MS.gene01386:CDS
AGGAAGTGCACTTGATCTGA+AGG 0.561598 2.2:+69535772 None:intergenic
CCACTTTCTGAATTACCACA+TGG 0.566899 2.2:+69536174 None:intergenic
TCTTCGTGGACATTTCTTTG+GGG 0.567519 2.2:-69534812 MS.gene01386:CDS
GCAATAGCTTGAGTCATCCA+TGG 0.568954 2.2:+69535084 None:intergenic
ATTTACTGCGAAATATTACA+AGG 0.570511 2.2:-69534411 MS.gene01386:CDS
GTTTCAGGAGGAGTTTGAGA+GGG 0.571950 2.2:-69534471 MS.gene01386:CDS
ACAATTACATCATACCATGT+TGG 0.575122 2.2:-69534579 MS.gene01386:CDS
AGTTTCAGGAGGAGTTTGAG+AGG 0.580705 2.2:-69534472 MS.gene01386:CDS
AGGAGGACCTATTGAGAATG+TGG 0.586619 2.2:-69534177 MS.gene01386:CDS
AGAATTATGCTAAAAGGAGT+GGG 0.587053 2.2:-69536041 MS.gene01386:CDS
TCATTACTGATCAAGATCCA+TGG 0.589067 2.2:-69535101 MS.gene01386:CDS
ATCTTCGAGATGTGGATGCA+AGG 0.590294 2.2:-69535890 MS.gene01386:CDS
GGTTCTCAATTAGAAAAGGT+AGG 0.597383 2.2:-69536020 MS.gene01386:CDS
TGCTTTCAAGAAGTTTCAGG+AGG 0.598760 2.2:-69534483 MS.gene01386:CDS
AGTCTTATGGGATGGTGATG+TGG 0.601149 2.2:-69534366 MS.gene01386:CDS
AAGCATAAAGTCTTATGGGA+TGG 0.601527 2.2:-69534374 MS.gene01386:CDS
AATAGACCATCTTCGAGATG+TGG 0.610642 2.2:-69535898 MS.gene01386:CDS
TACCATGTTGGATACATACA+GGG 0.612968 2.2:-69534567 MS.gene01386:CDS
GTAAACTTGCTCCTCTAAAG+GGG 0.614038 2.2:+69534523 None:intergenic
AATATGATGCTTAGTGAAGG+AGG 0.615302 2.2:-69534194 MS.gene01386:CDS
AAAACAAAACGATCAAAAGG+AGG 0.648569 2.2:-69534098 MS.gene01386:CDS
GTGAATATGATGCTTAGTGA+AGG 0.649082 2.2:-69534197 MS.gene01386:CDS
TCCCCTGTATGTATCCAACA+TGG 0.678677 2.2:+69534565 None:intergenic
AAATGTCCACGAAGATACGC+CGG 0.681339 2.2:+69534820 None:intergenic
AAGGTAGTAAATCATGATTG+TGG 0.681848 2.2:+69535791 None:intergenic
CAAGTTACTTGCCATTACGT+CGG 0.700694 2.2:-69534250 MS.gene01386:CDS
CTTCCAAACAATGAACCATG+TGG 0.706506 2.2:-69536189 MS.gene01386:CDS
ACCATGTTGGATACATACAG+GGG 0.713854 2.2:-69534566 MS.gene01386:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTTAATACAATTATTA+TGG - chr2.2:69534229-69534248 MS.gene01386:CDS 10.0%
!! TGAAATAAAAAAAATGATTA+AGG + chr2.2:69533990-69534009 None:intergenic 10.0%
!! AATCATATAGAAAATACATT+GGG + chr2.2:69535430-69535449 None:intergenic 15.0%
!!! ATTTATTCTAAATTTTAGGT+TGG - chr2.2:69535984-69536003 MS.gene01386:CDS 15.0%
!! AAAAGAACAAAAACAGAAAA+AGG + chr2.2:69534154-69534173 None:intergenic 20.0%
!! AAACAACAATTGAAAACAAA+AGG + chr2.2:69534087-69534106 None:intergenic 20.0%
!! AAAGAACAAAAACAGAAAAA+GGG + chr2.2:69534153-69534172 None:intergenic 20.0%
!! AACAAGTAAGAATTTACTTA+TGG + chr2.2:69534270-69534289 None:intergenic 20.0%
!! ACAAGTAAGAATTTACTTAT+GGG + chr2.2:69534269-69534288 None:intergenic 20.0%
!! ATAAAAGAAACTACAACAAT+GGG + chr2.2:69534177-69534196 None:intergenic 20.0%
!! CAATCATATAGAAAATACAT+TGG + chr2.2:69535431-69535450 None:intergenic 20.0%
!! TACATAAATAAATTGAAAGC+AGG + chr2.2:69534369-69534388 None:intergenic 20.0%
!! TATCAACAGTAAATTCAAAT+TGG + chr2.2:69535113-69535132 None:intergenic 20.0%
!! TGCTTTCAATTTATTTATGT+AGG - chr2.2:69534368-69534387 MS.gene01386:CDS 20.0%
!! TTTATGAGAATTATGCTAAA+AGG - chr2.2:69534566-69534585 MS.gene01386:CDS 20.0%
!!! AAAGACTCGAAAATATATTT+TGG - chr2.2:69535139-69535158 MS.gene01386:CDS 20.0%
!!! AATACTTATAATATGCCTTT+TGG - chr2.2:69535234-69535253 MS.gene01386:intron 20.0%
!!! GTAGGTTTGTTAATAAAAAT+GGG - chr2.2:69534611-69534630 MS.gene01386:CDS 20.0%
!!! TTTGAAACAGCATATTATAA+GGG + chr2.2:69534209-69534228 None:intergenic 20.0%
!!! TTTTGAAACAGCATATTATA+AGG + chr2.2:69534210-69534229 None:intergenic 20.0%
! AACAACCATATGAGTATAAA+TGG + chr2.2:69533948-69533967 None:intergenic 25.0%
! ACAAAGTTGTAAAAGATCAT+TGG + chr2.2:69535061-69535080 None:intergenic 25.0%
! ACAACCATATGAGTATAAAT+GGG + chr2.2:69533947-69533966 None:intergenic 25.0%
! ATAAAAATGGGATAATGCAT+AGG - chr2.2:69534623-69534642 MS.gene01386:intron 25.0%
! ATCACAAAACATTAACAAGA+TGG + chr2.2:69535948-69535967 None:intergenic 25.0%
! ATTCATCAAGATAACATGAA+TGG - chr2.2:69535030-69535049 MS.gene01386:CDS 25.0%
! ATTTACTGCGAAATATTACA+AGG - chr2.2:69536202-69536221 MS.gene01386:CDS 25.0%
! CTTGAGAAAAATGTAAAACA+TGG - chr2.2:69534961-69534980 MS.gene01386:CDS 25.0%
! GAAGACTTTAGATAAAAAAC+AGG + chr2.2:69536689-69536708 None:intergenic 25.0%
! GATAAAAGAAACTACAACAA+TGG + chr2.2:69534178-69534197 None:intergenic 25.0%
! TAAATAAATTGAAAGCAGGA+AGG + chr2.2:69534365-69534384 None:intergenic 25.0%
! TCTCAAAACAATCTTTATGT+AGG + chr2.2:69536337-69536356 None:intergenic 25.0%
! TTCGAAATAGATATTTCTCA+TGG - chr2.2:69535632-69535651 MS.gene01386:CDS 25.0%
!! ACAAAAGCATAAAGTCTTAT+GGG - chr2.2:69536235-69536254 MS.gene01386:intron 25.0%
!!! ATTGTTTTGAATTGTACCAA+CGG - chr2.2:69535172-69535191 MS.gene01386:intron 25.0%
!!! CAAAACATGCTTTTTGTATA+TGG - chr2.2:69535569-69535588 MS.gene01386:CDS 25.0%
!!! GGATCTTGAATTTTTTTATC+AGG + chr2.2:69534679-69534698 None:intergenic 25.0%
!!! GGTAGGTTTGTTAATAAAAA+TGG - chr2.2:69534610-69534629 MS.gene01386:CDS 25.0%
!!! TATACAAAAAGCATGTTTTG+TGG + chr2.2:69535569-69535588 None:intergenic 25.0%
!!! TTGATGATTTTGAAGAAGAA+TGG - chr2.2:69535692-69535711 MS.gene01386:CDS 25.0%
AAAACAAAACGATCAAAAGG+AGG - chr2.2:69536515-69536534 MS.gene01386:intron 30.0%
AAACAGTGTCTACATTTCAA+TGG - chr2.2:69535322-69535341 MS.gene01386:intron 30.0%
AAGGTAGTAAATCATGATTG+TGG + chr2.2:69534825-69534844 None:intergenic 30.0%
ACAACCCATTTATACTCATA+TGG - chr2.2:69533940-69533959 MS.gene01386:CDS 30.0%
ACAATTACATCATACCATGT+TGG - chr2.2:69536034-69536053 MS.gene01386:CDS 30.0%
ACTTAATTAAAAATCCGCCA+AGG - chr2.2:69536471-69536490 MS.gene01386:intron 30.0%
AGAATTATGCTAAAAGGAGT+GGG - chr2.2:69534572-69534591 MS.gene01386:CDS 30.0%
ATATGCTTTCAAGAAGTTTC+AGG - chr2.2:69536127-69536146 MS.gene01386:CDS 30.0%
ATCTTACCGCAAAATTTCTA+AGG - chr2.2:69534867-69534886 MS.gene01386:CDS 30.0%
ATTAAGGAATTAACTCACTC+AGG + chr2.2:69533974-69533993 None:intergenic 30.0%
CACATTACTGCTAAATTTAG+TGG - chr2.2:69535592-69535611 MS.gene01386:CDS 30.0%
CTATTTCGTTGTTCTTTAGA+CGG + chr2.2:69534700-69534719 None:intergenic 30.0%
GTATGAAGTTAAGCAACATT+GGG - chr2.2:69535768-69535787 MS.gene01386:CDS 30.0%
TACAATTCAAAACAATGAGG+TGG + chr2.2:69535168-69535187 None:intergenic 30.0%
TATTTGCTTGACTGAAAGAT+CGG + chr2.2:69534926-69534945 None:intergenic 30.0%
TGCATTTGATACTACTTACA+AGG - chr2.2:69535209-69535228 MS.gene01386:intron 30.0%
TGGTACAATTCAAAACAATG+AGG + chr2.2:69535171-69535190 None:intergenic 30.0%
TGTATGAAGTTAAGCAACAT+TGG - chr2.2:69535767-69535786 MS.gene01386:CDS 30.0%
TTACTGCTAAATTTAGTGGT+TGG - chr2.2:69535596-69535615 MS.gene01386:CDS 30.0%
! AACGATGTTGAACCTTTTAT+AGG - chr2.2:69534508-69534527 MS.gene01386:CDS 30.0%
! ACAACTTTGTAAGTTTTCCA+AGG - chr2.2:69535071-69535090 MS.gene01386:CDS 30.0%
! ACGATGTTGAACCTTTTATA+GGG - chr2.2:69534509-69534528 MS.gene01386:CDS 30.0%
! ATCTTCATTCTTCCTTTTCT+TGG + chr2.2:69536645-69536664 None:intergenic 30.0%
! CACAAAAGCATAAAGTCTTA+TGG - chr2.2:69536234-69536253 MS.gene01386:intron 30.0%
! CTTCTTCCTTAGAAATTTTG+CGG + chr2.2:69534876-69534895 None:intergenic 30.0%
! TAGGTTGAGAATCACAAATT+TGG - chr2.2:69534387-69534406 MS.gene01386:CDS 30.0%
!!! CCTTTTGATCGTTTTGTTTT+GGG + chr2.2:69536515-69536534 None:intergenic 30.0%
!!! TCCTTTTGATCGTTTTGTTT+TGG + chr2.2:69536516-69536535 None:intergenic 30.0%
AAAACGATCAAAAGGAGGAA+TGG - chr2.2:69536520-69536539 MS.gene01386:intron 35.0%
AAACAAAACCGAAACTGCAA+TGG + chr2.2:69534122-69534141 None:intergenic 35.0%
AACAAAACCGAAACTGCAAT+GGG + chr2.2:69534121-69534140 None:intergenic 35.0%
AATATGATGCTTAGTGAAGG+AGG - chr2.2:69536419-69536438 MS.gene01386:intron 35.0%
ACAGCATATTATAAGGGAAC+AGG + chr2.2:69534203-69534222 None:intergenic 35.0%
ACTCCAACAAAAATGCCAAA+AGG + chr2.2:69535252-69535271 None:intergenic 35.0%
AGAAAACACTGCCCTATAAA+AGG + chr2.2:69534523-69534542 None:intergenic 35.0%
AGCATCATATTCACTTCAAC+CGG + chr2.2:69536410-69536429 None:intergenic 35.0%
AGTAATGATTGTCTTGTCTC+TGG + chr2.2:69535500-69535519 None:intergenic 35.0%
ATACCATGTTGGATACATAC+AGG - chr2.2:69536045-69536064 MS.gene01386:CDS 35.0%
ATCTTCGTGGACATTTCTTT+GGG - chr2.2:69535800-69535819 MS.gene01386:CDS 35.0%
CAAGCAAATAATGCGTGTTA+TGG - chr2.2:69534936-69534955 MS.gene01386:CDS 35.0%
CATGAGAGTAACAAAAGTCT+GGG + chr2.2:69535468-69535487 None:intergenic 35.0%
CCCAAAACAAAACGATCAAA+AGG - chr2.2:69536512-69536531 MS.gene01386:intron 35.0%
GAAGCAAACAAGCACATAAA+AGG - chr2.2:69535742-69535761 MS.gene01386:CDS 35.0%
GAGAATTATGCTAAAAGGAG+TGG - chr2.2:69534571-69534590 MS.gene01386:CDS 35.0%
GGTTCTCAATTAGAAAAGGT+AGG - chr2.2:69534593-69534612 MS.gene01386:CDS 35.0%
GTATTCTCCACATTCTCAAT+AGG + chr2.2:69536446-69536465 None:intergenic 35.0%
GTGAATATGATGCTTAGTGA+AGG - chr2.2:69536416-69536435 MS.gene01386:intron 35.0%
GTTTGCTTCTAGATTGTACT+TGG + chr2.2:69535731-69535750 None:intergenic 35.0%
TACCATGTTGGATACATACA+GGG - chr2.2:69536046-69536065 MS.gene01386:CDS 35.0%
TATCTTCGTGGACATTTCTT+TGG - chr2.2:69535799-69535818 MS.gene01386:CDS 35.0%
TCATGAGAGTAACAAAAGTC+TGG + chr2.2:69535469-69535488 None:intergenic 35.0%
TCATTACTGATCAAGATCCA+TGG - chr2.2:69535512-69535531 MS.gene01386:intron 35.0%
TCGTGATGAATTTCAAAGAC+CGG - chr2.2:69536388-69536407 MS.gene01386:intron 35.0%
TGAACATGACTTTCTTTGTC+CGG + chr2.2:69536611-69536630 None:intergenic 35.0%
TTACCACATGGTTCATTGTT+TGG + chr2.2:69534430-69534449 None:intergenic 35.0%
TTTGAATTGTACCAACGGTA+TGG - chr2.2:69535177-69535196 MS.gene01386:intron 35.0%
! AAATTTTGCGGTAAGATGCA+AGG + chr2.2:69534864-69534883 None:intergenic 35.0%
! AAGCATAAAGTCTTATGGGA+TGG - chr2.2:69536239-69536258 MS.gene01386:intron 35.0%
! AGTGGGTTCTCAATTAGAAA+AGG - chr2.2:69534589-69534608 MS.gene01386:CDS 35.0%
! ATGCCTTTTGGCATTTTTGT+TGG - chr2.2:69535246-69535265 MS.gene01386:intron 35.0%
! CTCTTCTGGAAAAATCTCAA+AGG + chr2.2:69534776-69534795 None:intergenic 35.0%
! TGTAAACTTGCTCCTCTAAA+GGG + chr2.2:69536094-69536113 None:intergenic 35.0%
! TTCTTTTCCCATTGCAGTTT+CGG - chr2.2:69534111-69534130 MS.gene01386:CDS 35.0%
! TTGAGATTTTTCCAGAAGAG+TGG - chr2.2:69534776-69534795 MS.gene01386:CDS 35.0%
! TTGTAAACTTGCTCCTCTAA+AGG + chr2.2:69536095-69536114 None:intergenic 35.0%
! TTTCTAAGGAAGAAGAGAAG+TGG - chr2.2:69534881-69534900 MS.gene01386:CDS 35.0%
! TTTTATCAGGAAATCCTCCA+CGG + chr2.2:69534666-69534685 None:intergenic 35.0%
!! CAAAAAGCATGTTTTGTGGA+TGG + chr2.2:69535565-69535584 None:intergenic 35.0%
!! GAGATGAAGTAAAGCGTTTT+AGG + chr2.2:69535867-69535886 None:intergenic 35.0%
!! TTCCTTTTCTTGGTGATTGT+TGG + chr2.2:69536635-69536654 None:intergenic 35.0%
!! TTGCAAGCACTTTGAGTTTT+GGG - chr2.2:69536280-69536299 MS.gene01386:intron 35.0%
AAAGCAGGAAGGCTAAAATG+TGG + chr2.2:69534354-69534373 None:intergenic 40.0%
AATAGACCATCTTCGAGATG+TGG - chr2.2:69534715-69534734 MS.gene01386:intron 40.0%
AATGATTGTCTTGTCTCTGG+AGG + chr2.2:69535497-69535516 None:intergenic 40.0%
ACCATGTTGGATACATACAG+GGG - chr2.2:69536047-69536066 MS.gene01386:CDS 40.0%
AGCCAACAATCACCAAGAAA+AGG - chr2.2:69536630-69536649 MS.gene01386:intron 40.0%
AGTTAAGCAACATTGGGTTC+CGG - chr2.2:69535774-69535793 MS.gene01386:CDS 40.0%
CAAGTTACTTGCCATTACGT+CGG - chr2.2:69536363-69536382 MS.gene01386:intron 40.0%
CACGGCAGAACAATATTGTT+TGG - chr2.2:69535279-69535298 MS.gene01386:intron 40.0%
CCACTTTCTGAATTACCACA+TGG + chr2.2:69534442-69534461 None:intergenic 40.0%
CCATGTGGTAATTCAGAAAG+TGG - chr2.2:69534439-69534458 MS.gene01386:CDS 40.0%
CTTCCAAACAATGAACCATG+TGG - chr2.2:69534424-69534443 MS.gene01386:CDS 40.0%
GCACAATGTTACAACTTGTC+CGG - chr2.2:69536589-69536608 MS.gene01386:intron 40.0%
TCGATCTTTATCCCCTTTAG+AGG - chr2.2:69536079-69536098 MS.gene01386:CDS 40.0%
TCTTCGTGGACATTTCTTTG+GGG - chr2.2:69535801-69535820 MS.gene01386:CDS 40.0%
TGCTTTCAAGAAGTTTCAGG+AGG - chr2.2:69536130-69536149 MS.gene01386:CDS 40.0%
TTTGTTGGAGTCGACAATCA+CGG - chr2.2:69535261-69535280 MS.gene01386:intron 40.0%
! CCTTTTGTATTAGACCTTGG+CGG + chr2.2:69536488-69536507 None:intergenic 40.0%
! CGACCTTTTGTATTAGACCT+TGG + chr2.2:69536491-69536510 None:intergenic 40.0%
! CGTAATGGCAAGTAACTTGT+TGG + chr2.2:69536362-69536381 None:intergenic 40.0%
! CTGTACTTTTCGCAAGAGAA+GGG - chr2.2:69536565-69536584 MS.gene01386:intron 40.0%
! GAAGCATGAGTTTTCTTCCT+TGG + chr2.2:69535091-69535110 None:intergenic 40.0%
! GGAAGATCAGAGAGCATAAA+TGG - chr2.2:69535838-69535857 MS.gene01386:CDS 40.0%
! GTAAACTTGCTCCTCTAAAG+GGG + chr2.2:69536093-69536112 None:intergenic 40.0%
! TGTACTTTTCGCAAGAGAAG+GGG - chr2.2:69536566-69536585 MS.gene01386:intron 40.0%
! TTTTTGCCCAATGTCCTCAA+TGG + chr2.2:69536015-69536034 None:intergenic 40.0%
!! GTTGCAAGCACTTTGAGTTT+TGG - chr2.2:69536279-69536298 MS.gene01386:intron 40.0%
!! TGCAAGCACTTTGAGTTTTG+GGG - chr2.2:69536281-69536300 MS.gene01386:intron 40.0%
!!! AGAGATTTGTTTTGTCACCG+TGG - chr2.2:69534646-69534665 MS.gene01386:intron 40.0%
AAATGTCCACGAAGATACGC+CGG + chr2.2:69535796-69535815 None:intergenic 45.0%
AGGAGGACCTATTGAGAATG+TGG - chr2.2:69536436-69536455 MS.gene01386:intron 45.0%
AGTTTCAGGAGGAGTTTGAG+AGG - chr2.2:69536141-69536160 MS.gene01386:CDS 45.0%
ATCTTCGAGATGTGGATGCA+AGG - chr2.2:69534723-69534742 MS.gene01386:CDS 45.0%
CAACAACATCTCCATACCGT+TGG + chr2.2:69535191-69535210 None:intergenic 45.0%
CCGCCAAGGTCTAATACAAA+AGG - chr2.2:69536485-69536504 MS.gene01386:intron 45.0%
CTTCGTGGACATTTCTTTGG+GGG - chr2.2:69535802-69535821 MS.gene01386:CDS 45.0%
GCAATAGCTTGAGTCATCCA+TGG + chr2.2:69535532-69535551 None:intergenic 45.0%
GTCTTGCCATTGAGGACATT+GGG - chr2.2:69536006-69536025 MS.gene01386:CDS 45.0%
GTTTCAGGAGGAGTTTGAGA+GGG - chr2.2:69536142-69536161 MS.gene01386:CDS 45.0%
TCAATAACCTGCCACTCTTC+TGG + chr2.2:69534790-69534809 None:intergenic 45.0%
TCCCCTGTATGTATCCAACA+TGG + chr2.2:69536051-69536070 None:intergenic 45.0%
TTGCATCCACATCTCGAAGA+TGG + chr2.2:69534724-69534743 None:intergenic 45.0%
! AGGAAGTGCACTTGATCTGA+AGG + chr2.2:69534844-69534863 None:intergenic 45.0%
! AGGGCAGTGTTTTCTAAGTG+AGG - chr2.2:69534528-69534547 MS.gene01386:CDS 45.0%
! AGTCTTATGGGATGGTGATG+TGG - chr2.2:69536247-69536266 MS.gene01386:intron 45.0%
! GATTTTTCCAGAAGAGTGGC+AGG - chr2.2:69534780-69534799 MS.gene01386:CDS 45.0%
! GCTGTACTTTTCGCAAGAGA+AGG - chr2.2:69536564-69536583 MS.gene01386:intron 45.0%
! GTACTTTTCGCAAGAGAAGG+GGG - chr2.2:69536567-69536586 MS.gene01386:intron 45.0%
! TTATGGGATGGTGATGTGGT+TGG - chr2.2:69536251-69536270 MS.gene01386:intron 45.0%
!! TTAGGTTGGTCTTGCCATTG+AGG - chr2.2:69535998-69536017 MS.gene01386:CDS 45.0%
!!! GATTTGTTTTGTCACCGTGG+AGG - chr2.2:69534649-69534668 MS.gene01386:intron 45.0%
!!! ATAAATTTATTCTAAATTTT+AGG - chr2.2:69535980-69535999 MS.gene01386:CDS 5.0%
GGTCTTGCCATTGAGGACAT+TGG - chr2.2:69536005-69536024 MS.gene01386:CDS 50.0%
TCATCACGACACCGACGTAA+TGG + chr2.2:69536377-69536396 None:intergenic 50.0%
! CTTTGGGGGCATGACAACAA+CGG - chr2.2:69535816-69535835 MS.gene01386:CDS 50.0%
! TTTGGGGGCATGACAACAAC+GGG - chr2.2:69535817-69535836 MS.gene01386:CDS 50.0%
TGGGTTCCGGCGTATCTTCG+TGG - chr2.2:69535787-69535806 MS.gene01386:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 69533923 69536712 69533923 ID=MS.gene01386
chr2.2 mRNA 69533923 69536712 69533923 ID=MS.gene01386.t1;Parent=MS.gene01386
chr2.2 exon 69536660 69536712 69536660 ID=MS.gene01386.t1.exon1;Parent=MS.gene01386.t1
chr2.2 CDS 69536660 69536712 69536660 ID=cds.MS.gene01386.t1;Parent=MS.gene01386.t1
chr2.2 exon 69535557 69536245 69535557 ID=MS.gene01386.t1.exon2;Parent=MS.gene01386.t1
chr2.2 CDS 69535557 69536245 69535557 ID=cds.MS.gene01386.t1;Parent=MS.gene01386.t1
chr2.2 exon 69534720 69535165 69534720 ID=MS.gene01386.t1.exon3;Parent=MS.gene01386.t1
chr2.2 CDS 69534720 69535165 69534720 ID=cds.MS.gene01386.t1;Parent=MS.gene01386.t1
chr2.2 exon 69533923 69534633 69533923 ID=MS.gene01386.t1.exon4;Parent=MS.gene01386.t1
chr2.2 CDS 69533923 69534633 69533923 ID=cds.MS.gene01386.t1;Parent=MS.gene01386.t1
Gene Sequence

>MS.gene01386

ATGGCAAGTGAAAAAGAACAACCCATTTATACTCATATGGTTGTTGTACCTGAGTGAGTTAATTCCTTAATCATTTTTTTTATTTCACGTTTTTTTTTCTTCTTCATTGTTCTAGTTCTTTGTTTCAATTGTTCTTCATTTGTTATTCAATTGTTATTTCACCTTTTGTTTTCAATTGTTGTTTTTTGTTCTTTTCCCATTGCAGTTTCGGTTTTGTTTATGTTGTTCCCTTTTTCTGTTTTTGTTCTTTTCCCATTGTTGTAGTTTCTTTTATCTGCCTGTTCCCTTATAATATGCTGTTTCAAAAAAAGTTAATACAATTATTATGGCAAGTGAAAAAGAACCCATAAGTAAATTCTTACTTGTTTTTTCATGCATGTTAATACATTTCTTATTAGTTGTTCTTTCAATTGCTTTGCAACTCTACTCCACATTTTAGCCTTCCTGCTTTCAATTTATTTATGTAGGTTGAGAATCACAAATTTGGTTGATTTGAACAATCTTCCAAACAATGAACCATGTGGTAATTCAGAAAGTGGAGTAGTTGAGTTGAGTAGCAATGAACAAGAAGCAATTACTATTGAAAACGATGTTGAACCTTTTATAGGGCAGTGTTTTCTAAGTGAGGAAGAAGCTTTCAAATTTTATGAGAATTATGCTAAAAGGAGTGGGTTCTCAATTAGAAAAGGTAGGTTTGTTAATAAAAATGGGATAATGCATAGGAGAGATTTGTTTTGTCACCGTGGAGGATTTCCTGATAAAAAAATTCAAGATCCGTCTAAAGAACAACGAAATAGACCATCTTCGAGATGTGGATGCAAGGCAAGCATGACAATCAAATTGAAGAAATCCTTTGAGATTTTTCCAGAAGAGTGGCAGGTTATTGAATTTATATCTAACCACAATCATGATTTACTACCTTCAGATCAAGTGCACTTCCTTGCATCTTACCGCAAAATTTCTAAGGAAGAAGAGAAGTGGATATTGCTATTAAAAGAAGCCGATCTTTCAGTCAAGCAAATAATGCGTGTTATGGAGCTTGAGAAAAATGTAAAACATGGAGATCTTCGATTTCTTGAGAAAGATATTCATAATTTCTTTAATAAGATTCATCAAGATAACATGAATGGTGATGCCAATGATCTTTTACAACTTTGTAAGTTTTCCAAGGAAGAAAACTCATGCTTCCAATTTGAATTTACTGTTGATAAAGAAAAAAGACTCGAAAATATATTTTGGTCTCCACCTCATTGTTTTGAATTGTACCAACGGTATGGAGATGTTGTTGCATTTGATACTACTTACAAGGTAAATACTTATAATATGCCTTTTGGCATTTTTGTTGGAGTCGACAATCACGGCAGAACAATATTGTTTGGTTGTGCGCTCATTCGAAATGAAACAGTGTCTACATTTCAATGGTTAATGAAAGTAAGTTCATATACATCTCTCTCTTTTGTTATTAACATTCATAGACTATTATTTTCATCTTTTTATATAGTATCCCAATGTATTTTCTATATGATTGTTAGTATTTTTTTTCCCAGACTTTTGTTACTCTCATGAAGAAGCCTCCAGAGACAAGACAATCATTACTGATCAAGATCCATGGATGACTCAAGCTATTGCTTTTGAAATGCCATCCACAAAACATGCTTTTTGTATATGGCACATTACTGCTAAATTTAGTGGTTGGTTTACTTCAATTCTTCGAAATAGATATTTCTCATGGTGTGCTGATTTTTATAAGCTTTACAAGCTTGACAATATTGATGATTTTGAAGAAGAATGGATTATAATTGTTTCCAAGTACAATCTAGAAGCAAACAAGCACATAAAAGGCTTGTATGAAGTTAAGCAACATTGGGTTCCGGCGTATCTTCGTGGACATTTCTTTGGGGGCATGACAACAACGGGAAGATCAGAGAGCATAAATGGATTCCTAAAACGCTTTACTTCATCTCGTTCATGTTTAACTCAACTTTTCAAACAAGTAAGAATATGTATGATAATTTAGTTTAGCCATCTTGTTAATGTTTTGTGATGTAGATTTGACTATAAATTTATTCTAAATTTTAGGTTGGTCTTGCCATTGAGGACATTGGGCAAAAACAATTACATCATACCATGTTGGATACATACAGGGGATCATCACTTCGATCTTTATCCCCTTTAGAGGAGCAAGTTTACAATATTTTCACAAAATATGCTTTCAAGAAGTTTCAGGAGGAGTTTGAGAGGGCAACTCAATACAAGATTTGTGAAGAAAATCATGTAGAATTTACTGCGAAATATTACAAGGAGCAACATTCACAAAAGCATAAAGTCTTATGGGATGGTGATGTGGTTGGTTGTAGTTGCAAGCACTTTGAGTTTTGGGGTATTCTTTGTCGTCATGTATTGACAATATTCCTACATAAAGATTGTTTTGAGATTCCAACAAGTTACTTGCCATTACGTCGGTGTCGTGATGAATTTCAAAGACCGGTTGAAGTGAATATGATGCTTAGTGAAGGAGGACCTATTGAGAATGTGGAGAATACAGTAGACTTAATTAAAAATCCGCCAAGGTCTAATACAAAAGGTCGTCCCAAAACAAAACGATCAAAAGGAGGAATGGAATTGTCAAAGAGAGCAAGAAGCTGTACTTTTCGCAAGAGAAGGGGGCACAATGTTACAACTTGTCCGGACAAAGAAAGTCATGTTCAGCCAACAATCACCAAGAAAAGGAAGAATGAAGATCAAGCACAACAAAATTTGAATCCTGTTTTTTATCTAAAGTCTTCTTAG
Protein sequence

>MS.gene01386.t1

MASEKEQPIYTHMVVVPELRITNLVDLNNLPNNEPCGNSESGVVELSSNEQEAITIENDVEPFIGQCFLSEEEAFKFYENYAKRSGFSIRKGRFVNKNGIMHRRDLFCHRGGFPDKKIQDPSKEQRNRPSSRCGCKASMTIKLKKSFEIFPEEWQVIEFISNHNHDLLPSDQVHFLASYRKISKEEEKWILLLKEADLSVKQIMRVMELEKNVKHGDLRFLEKDIHNFFNKIHQDNMNGDANDLLQLYFCYSHEEASRDKTIITDQDPWMTQAIAFEMPSTKHAFCIWHITAKFSGWFTSILRNRYFSWCADFYKLYKLDNIDDFEEEWIIIVSKYNLEANKHIKGLYEVKQHWVPAYLRGHFFGGMTTTGRSESINGFLKRFTSSRSCLTQLFKQVGLAIEDIGQKQLHHTMLDTYRGSSLRSLSPLEEQVYNIFTKYAFKKFQEEFERATQYKICEENHVEFTAKYYKEQHSQKHKVLWDGDVVGCSCKHFEFWGILCRHVLTIFLHKDCFEIPTSYLPLRRCRDEFQRPVEVNMMLSEGGPIENVENTVDLIKNPPRSNTKGRPKTKRSKGGMELSKRARSCTFRKRRGHNVTTCPDKESHVQPTITKKRKNEDQAQQNLNPVFYLKSS