Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013949.t1 | RHN47673.1 | 88 | 234 | 25 | 1 | 1 | 231 | 1 | 234 | 3.80E-108 | 401 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013949.t1 | Q39261 | 38.6 | 88 | 41 | 4 | 78 | 162 | 50 | 127 | 1.6e-06 | 54.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013949.t1 | A0A396H4J8 | 88.0 | 234 | 25 | 1 | 1 | 231 | 1 | 234 | 2.7e-108 | 401.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene013949.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013949.t1 | MTR_7g022250 | 78.243 | 239 | 44 | 3 | 1 | 231 | 1 | 239 | 6.76e-137 | 384 |
| MS.gene013949.t1 | MTR_7g028890 | 79.060 | 234 | 32 | 2 | 1 | 231 | 1 | 220 | 4.67e-131 | 368 |
| MS.gene013949.t1 | MTR_7g028915 | 75.641 | 234 | 32 | 2 | 1 | 231 | 1 | 212 | 1.33e-124 | 352 |
| MS.gene013949.t1 | MTR_7g022270 | 58.854 | 192 | 41 | 4 | 22 | 211 | 6 | 161 | 7.00e-72 | 216 |
| MS.gene013949.t1 | MTR_4g128340 | 28.846 | 156 | 80 | 3 | 77 | 231 | 44 | 169 | 1.99e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013949.t1 | AT1G66140 | 29.670 | 182 | 98 | 4 | 77 | 231 | 82 | 260 | 4.83e-11 | 61.2 |
Find 43 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTGGATATCATTATTA+TGG | 0.231665 | 7.2:-19922393 | MS.gene013949:CDS |
| AAGAAATGGAGAAATTTCTT+TGG | 0.320928 | 7.2:+19922641 | None:intergenic |
| CTCCTTCCCCTTGTTGCACA+TGG | 0.338672 | 7.2:+19922812 | None:intergenic |
| CAAAGCATAATGAGTCAAAA+AGG | 0.368100 | 7.2:-19922264 | MS.gene013949:CDS |
| TCCAAGCATTGTTAAAAGCT+TGG | 0.375606 | 7.2:+19922475 | None:intergenic |
| CACCATCTCTCCATTCCATA+TGG | 0.384400 | 7.2:-19922415 | MS.gene013949:CDS |
| ATATATATAATCAAAGCTTA+AGG | 0.394278 | 7.2:+19922127 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | 7.2:+19922143 | None:intergenic |
| AATGATATCCACACCCCATA+TGG | 0.402297 | 7.2:+19922400 | None:intergenic |
| AATTACTCAAATGGTGATCA+AGG | 0.411277 | 7.2:-19922450 | MS.gene013949:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7.2:-19922366 | MS.gene013949:CDS |
| ATGTTGAAACTCAGAATTCA+AGG | 0.446337 | 7.2:-19922707 | MS.gene013949:CDS |
| GCTTGGATAAATTACTCAAA+TGG | 0.450589 | 7.2:-19922459 | MS.gene013949:CDS |
| AGTAGCCAATTTGAAGATGA+TGG | 0.452167 | 7.2:-19922772 | MS.gene013949:CDS |
| TCATCTGCTTCATCTATCTC+TGG | 0.464587 | 7.2:+19922294 | None:intergenic |
| ACCATCTCTCCATTCCATAT+GGG | 0.464694 | 7.2:-19922414 | MS.gene013949:CDS |
| TATATATAATCAAAGCTTAA+GGG | 0.466620 | 7.2:+19922128 | None:intergenic |
| TCACAGGAAAAGTATTCAAA+AGG | 0.468253 | 7.2:-19922598 | MS.gene013949:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7.2:-19922546 | MS.gene013949:CDS |
| TGAAGTTGGTTCAGTTTCAA+AGG | 0.476665 | 7.2:-19922226 | MS.gene013949:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7.2:-19922614 | MS.gene013949:CDS |
| CAAGAGAAAGGTAATTGTGC+TGG | 0.486649 | 7.2:-19922739 | MS.gene013949:CDS |
| AATATGTCTGAGATTGAAGT+TGG | 0.493412 | 7.2:-19922240 | MS.gene013949:CDS |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7.2:-19922196 | MS.gene013949:CDS |
| CTTTGATGCCATGTGCAACA+AGG | 0.514941 | 7.2:-19922820 | None:intergenic |
| GAAGTTGGTTCAGTTTCAAA+GGG | 0.529917 | 7.2:-19922225 | MS.gene013949:CDS |
| TCTCCATTCCATATGGGGTG+TGG | 0.536840 | 7.2:-19922408 | MS.gene013949:CDS |
| GGAAAATCAGAACAAGAGAA+AGG | 0.553067 | 7.2:-19922751 | MS.gene013949:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7.2:-19922365 | MS.gene013949:CDS |
| CCCCATATGGAATGGAGAGA+TGG | 0.561790 | 7.2:+19922413 | None:intergenic |
| TGCCATGTGCAACAAGGGGA+AGG | 0.572549 | 7.2:-19922814 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7.2:-19922553 | MS.gene013949:CDS |
| TTTGATGCCATGTGCAACAA+GGG | 0.577704 | 7.2:-19922819 | None:intergenic |
| GTGCAACAAGGGGAAGGAGA+AGG | 0.585340 | 7.2:-19922808 | MS.gene013949:CDS |
| GTTGGTTCAGTTTCAAAGGG+AGG | 0.593873 | 7.2:-19922222 | MS.gene013949:CDS |
| TATCCACACCCCATATGGAA+TGG | 0.594771 | 7.2:+19922405 | None:intergenic |
| CCATCTCTCCATTCCATATG+GGG | 0.598148 | 7.2:-19922413 | MS.gene013949:CDS |
| ATTACTCAAATGGTGATCAA+GGG | 0.605537 | 7.2:-19922449 | MS.gene013949:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7.2:-19922175 | MS.gene013949:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.637178 | 7.2:+19922537 | None:intergenic |
| TTGATGCCATGTGCAACAAG+GGG | 0.659240 | 7.2:-19922818 | None:intergenic |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7.2:-19922543 | MS.gene013949:CDS |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7.2:-19922172 | MS.gene013949:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | + | chr7.2:19922309-19922328 | None:intergenic | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | + | chr7.2:19922323-19922342 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | - | chr7.2:19922314-19922333 | MS.gene013949:CDS | 25.0% |
| !! | ATTTTCCATCATCTTCAAAT+TGG | + | chr7.2:19922183-19922202 | None:intergenic | 25.0% |
| !! | TTCTTCAACAACATCTTTTT+GGG | + | chr7.2:19922270-19922289 | None:intergenic | 25.0% |
| AATTACTCAAATGGTGATCA+AGG | - | chr7.2:19922497-19922516 | MS.gene013949:CDS | 30.0% | |
| ATGTTGAAACTCAGAATTCA+AGG | - | chr7.2:19922240-19922259 | MS.gene013949:CDS | 30.0% | |
| ATTACTCAAATGGTGATCAA+GGG | - | chr7.2:19922498-19922517 | MS.gene013949:CDS | 30.0% | |
| CAAAGCATAATGAGTCAAAA+AGG | - | chr7.2:19922683-19922702 | MS.gene013949:CDS | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | - | chr7.2:19922488-19922507 | MS.gene013949:CDS | 30.0% | |
| TAGAGAGAAGTCTCAAAAAA+AGG | - | chr7.2:19922447-19922466 | MS.gene013949:CDS | 30.0% | |
| ! | AATATGTCTGAGATTGAAGT+TGG | - | chr7.2:19922707-19922726 | MS.gene013949:CDS | 30.0% |
| ! | CTTCTTCAACAACATCTTTT+TGG | + | chr7.2:19922271-19922290 | None:intergenic | 30.0% |
| ! | TCTTCAACAACATCTTTTTG+GGG | + | chr7.2:19922269-19922288 | None:intergenic | 30.0% |
| ! | TTTTATGCAGGGAATTTGTA+TGG | - | chr7.2:19922614-19922633 | MS.gene013949:CDS | 30.0% |
| !! | TCACAGGAAAAGTATTCAAA+AGG | - | chr7.2:19922349-19922368 | MS.gene013949:CDS | 30.0% |
| AACAATATGATTCAGCATGC+AGG | - | chr7.2:19922581-19922600 | MS.gene013949:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | - | chr7.2:19922582-19922601 | MS.gene013949:CDS | 35.0% | |
| AGTAGCCAATTTGAAGATGA+TGG | - | chr7.2:19922175-19922194 | MS.gene013949:CDS | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | + | chr7.2:19922807-19922826 | None:intergenic | 35.0% | |
| GGAAAATCAGAACAAGAGAA+AGG | - | chr7.2:19922196-19922215 | MS.gene013949:CDS | 35.0% | |
| GGGTGTGGATATCATTATTA+TGG | - | chr7.2:19922554-19922573 | MS.gene013949:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | - | chr7.2:19922333-19922352 | MS.gene013949:CDS | 35.0% | |
| ! | GAAGTTGGTTCAGTTTCAAA+GGG | - | chr7.2:19922722-19922741 | MS.gene013949:CDS | 35.0% |
| ! | GGTCATCAAACTTTTATGCA+GGG | - | chr7.2:19922603-19922622 | MS.gene013949:CDS | 35.0% |
| ! | TCCAAGCATTGTTAAAAGCT+TGG | + | chr7.2:19922475-19922494 | None:intergenic | 35.0% |
| ! | TCCAAGCTTTTAACAATGCT+TGG | - | chr7.2:19922471-19922490 | MS.gene013949:CDS | 35.0% |
| ! | TGAAGTTGGTTCAGTTTCAA+AGG | - | chr7.2:19922721-19922740 | MS.gene013949:CDS | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | - | chr7.2:19922751-19922770 | MS.gene013949:CDS | 35.0% |
| AATGATATCCACACCCCATA+TGG | + | chr7.2:19922550-19922569 | None:intergenic | 40.0% | |
| ACCATCTCTCCATTCCATAT+GGG | - | chr7.2:19922533-19922552 | MS.gene013949:CDS | 40.0% | |
| CAAGAGAAAGGTAATTGTGC+TGG | - | chr7.2:19922208-19922227 | MS.gene013949:CDS | 40.0% | |
| TCATCTGCTTCATCTATCTC+TGG | + | chr7.2:19922656-19922675 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | - | chr7.2:19922602-19922621 | MS.gene013949:CDS | 40.0% |
| AGACAAAGAGACTCAAGAGG+AGG | - | chr7.2:19922775-19922794 | MS.gene013949:CDS | 45.0% | |
| CACCATCTCTCCATTCCATA+TGG | - | chr7.2:19922532-19922551 | MS.gene013949:CDS | 45.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | - | chr7.2:19922394-19922413 | MS.gene013949:CDS | 45.0% | |
| CCATCTCTCCATTCCATATG+GGG | - | chr7.2:19922534-19922553 | MS.gene013949:CDS | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | - | chr7.2:19922772-19922791 | MS.gene013949:CDS | 45.0% | |
| TATCCACACCCCATATGGAA+TGG | + | chr7.2:19922545-19922564 | None:intergenic | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | - | chr7.2:19922401-19922420 | MS.gene013949:CDS | 45.0% | |
| ! | GTTGGTTCAGTTTCAAAGGG+AGG | - | chr7.2:19922725-19922744 | MS.gene013949:CDS | 45.0% |
| ACTAGTCCACAGGCATTAGG+TGG | - | chr7.2:19922404-19922423 | MS.gene013949:CDS | 50.0% | |
| CCCCATATGGAATGGAGAGA+TGG | + | chr7.2:19922537-19922556 | None:intergenic | 50.0% | |
| TCTCCATTCCATATGGGGTG+TGG | - | chr7.2:19922539-19922558 | MS.gene013949:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | + | chr7.2:19922413-19922432 | None:intergenic | 50.0% | |
| GTGCAACAAGGGGAAGGAGA+AGG | - | chr7.2:19922139-19922158 | MS.gene013949:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 19922137 | 19922832 | 19922137 | ID=MS.gene013949 |
| chr7.2 | mRNA | 19922137 | 19922832 | 19922137 | ID=MS.gene013949.t1;Parent=MS.gene013949 |
| chr7.2 | exon | 19922137 | 19922832 | 19922137 | ID=MS.gene013949.t1.exon1;Parent=MS.gene013949.t1 |
| chr7.2 | CDS | 19922137 | 19922832 | 19922137 | ID=cds.MS.gene013949.t1;Parent=MS.gene013949.t1 |
| Gene Sequence |
| Protein sequence |
