AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013966

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013966.t1 MTR_7g089010 95.395 304 6 4 1 301 1 299 0.0 570
MS.gene013966.t1 MTR_4g064730 44.292 219 65 4 5 182 8 210 3.92e-44 153
MS.gene013966.t1 MTR_4g121020 67.647 68 22 0 86 153 106 173 2.86e-25 105
MS.gene013966.t1 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.36e-25 105
MS.gene013966.t1 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.39e-25 105
MS.gene013966.t1 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.34e-22 98.2
MS.gene013966.t1 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.39e-22 98.2
MS.gene013966.t1 MTR_2g034960 60.294 68 27 0 86 153 206 273 5.23e-22 96.3
MS.gene013966.t1 MTR_3g106220 54.545 77 34 1 78 154 186 261 5.88e-22 96.3
MS.gene013966.t1 MTR_7g117705 63.636 66 21 1 90 155 190 252 3.51e-21 93.6
MS.gene013966.t1 MTR_1g032570 60.938 64 25 0 90 153 130 193 7.56e-21 92.8
MS.gene013966.t1 MTR_1g032570 60.938 64 25 0 90 153 199 262 9.16e-21 92.8
MS.gene013966.t1 MTR_1g013170 65.000 60 21 0 90 149 190 249 3.75e-20 90.5
MS.gene013966.t1 MTR_7g026400 34.559 136 75 3 82 217 148 269 4.98e-20 90.5
MS.gene013966.t1 MTR_4g131570 65.574 61 21 0 88 148 200 260 6.02e-20 90.5
MS.gene013966.t1 MTR_4g131600 54.412 68 31 0 82 149 192 259 2.22e-19 88.6
MS.gene013966.t1 MTR_1g013180 54.930 71 32 0 90 160 341 411 4.13e-19 87.8
MS.gene013966.t1 MTR_0450s0040 52.857 70 33 0 88 157 166 235 4.72e-19 86.3
MS.gene013966.t1 MTR_4g131580 65.574 61 21 0 88 148 197 257 4.78e-19 87.8
MS.gene013966.t1 MTR_3g102600 54.545 66 28 1 90 155 82 145 4.13e-18 80.5
MS.gene013966.t1 MTR_3g102600 54.545 66 28 1 90 155 131 194 6.83e-18 81.3
MS.gene013966.t1 MTR_8g079940 56.164 73 28 1 89 157 207 279 2.47e-17 82.4
MS.gene013966.t1 MTR_3g086100 51.389 72 35 0 90 161 127 198 6.60e-17 81.3
MS.gene013966.t1 MTR_3g086100 51.389 72 35 0 90 161 198 269 8.55e-17 80.9
MS.gene013966.t1 MTR_5g014040 56.667 60 26 0 90 149 310 369 1.65e-16 80.1
MS.gene013966.t1 MTR_4g098870 55.000 60 27 0 90 149 309 368 7.53e-16 78.2
MS.gene013966.t1 MTR_4g098870 55.000 60 27 0 90 149 329 388 7.76e-16 78.2
MS.gene013966.t1 MTR_4g098870 58.929 56 23 0 90 145 309 364 7.86e-16 78.2
MS.gene013966.t1 MTR_4g098870 58.929 56 23 0 90 145 329 384 8.17e-16 78.2
MS.gene013966.t1 MTR_4g098870 55.000 60 27 0 90 149 348 407 8.75e-16 77.8
MS.gene013966.t1 MTR_4g086835 51.515 66 32 0 84 149 267 332 1.01e-15 77.4
MS.gene013966.t1 MTR_4g021760 56.897 58 25 0 90 147 168 225 1.74e-15 75.9
MS.gene013966.t1 MTR_4g078810 51.667 60 29 0 90 149 157 216 2.16e-15 76.3
MS.gene013966.t1 MTR_2g090675 33.566 143 59 4 90 219 160 279 2.33e-15 76.3
MS.gene013966.t1 MTR_3g102590 44.304 79 42 1 90 166 142 220 2.57e-15 74.3
MS.gene013966.t1 MTR_5g014040 52.308 65 26 1 90 149 270 334 1.67e-14 73.9
MS.gene013966.t1 MTR_4g086835 50.746 67 32 1 84 149 267 333 1.73e-14 73.6
MS.gene013966.t1 MTR_5g014040 52.308 65 26 1 90 149 310 374 1.84e-14 73.9
MS.gene013966.t1 MTR_5g017980 52.542 59 28 0 90 148 191 249 4.51e-14 72.0
MS.gene013966.t1 MTR_5g017980 52.542 59 28 0 90 148 192 250 4.91e-14 71.6
MS.gene013966.t1 MTR_4g021790 54.237 59 27 0 90 148 167 225 6.80e-14 70.9
MS.gene013966.t1 MTR_4g113140 48.571 70 35 1 80 148 224 293 7.74e-14 71.6
MS.gene013966.t1 MTR_8g105600 57.895 57 24 0 94 150 190 246 1.09e-13 71.6
MS.gene013966.t1 MTR_5g041350 56.364 55 23 1 91 144 15 69 1.68e-13 70.5
MS.gene013966.t1 MTR_2g086450 55.385 65 27 2 91 154 45 108 4.15e-13 69.3
MS.gene013966.t1 MTR_6g032990 40.000 95 45 3 91 181 42 128 1.16e-12 68.2
MS.gene013966.t1 MTR_5g017980 51.667 60 28 1 90 148 192 251 1.20e-12 67.8
MS.gene013966.t1 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.42e-12 67.8
MS.gene013966.t1 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.46e-12 67.8
MS.gene013966.t1 MTR_1g090670 46.429 84 41 2 81 160 31 114 1.54e-12 67.0
MS.gene013966.t1 MTR_2g027800 50.685 73 35 1 91 162 39 111 1.80e-12 67.0
MS.gene013966.t1 MTR_1g093080 50.000 60 29 1 90 148 210 269 1.96e-12 67.4
MS.gene013966.t1 MTR_7g098250 50.725 69 31 2 91 158 262 328 2.06e-12 67.8
MS.gene013966.t1 MTR_4g021855 52.542 59 28 0 90 148 167 225 2.09e-12 66.6
MS.gene013966.t1 MTR_5g054300 51.667 60 28 1 90 148 197 256 3.14e-12 66.6
MS.gene013966.t1 MTR_1g053830 52.830 53 24 1 93 144 20 72 3.14e-12 62.4
MS.gene013966.t1 MTR_5g027440 56.364 55 23 1 91 144 25 79 3.87e-12 65.9
MS.gene013966.t1 MTR_3g102600 47.170 53 28 0 90 142 82 134 4.59e-12 63.2
MS.gene013966.t1 MTR_2g027800 60.000 55 21 1 91 144 39 93 5.45e-12 65.5
MS.gene013966.t1 MTR_3g102600 47.170 53 28 0 90 142 131 183 7.06e-12 63.5
MS.gene013966.t1 MTR_1g080330 55.556 54 23 1 91 143 175 228 9.05e-12 65.5
MS.gene013966.t1 MTR_1g080330 55.556 54 23 1 91 143 175 228 1.00e-11 64.7
MS.gene013966.t1 MTR_6g045327 48.276 58 30 0 90 147 171 228 1.16e-11 65.1
MS.gene013966.t1 MTR_4g081710 52.727 55 25 1 91 144 17 71 1.64e-11 64.3
MS.gene013966.t1 MTR_2g027860 58.182 55 22 1 91 144 49 103 1.66e-11 64.3
MS.gene013966.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.03e-11 63.9
MS.gene013966.t1 MTR_8g066310 41.905 105 54 4 43 143 125 226 2.45e-11 63.9
MS.gene013966.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.76e-11 63.5
MS.gene013966.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.25e-11 63.9
MS.gene013966.t1 MTR_7g088070 42.857 105 46 4 78 170 37 139 3.57e-11 62.8
MS.gene013966.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.88e-11 63.5
MS.gene013966.t1 MTR_0223s0040 56.364 55 23 1 90 143 17 71 8.50e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013966.t1 AT3G10760 54.286 350 96 10 1 301 1 335 6.72e-90 271
MS.gene013966.t1 AT5G05090 54.369 309 90 10 1 301 1 266 3.06e-79 241
MS.gene013966.t1 AT2G40970 61.682 214 57 6 1 199 1 204 1.01e-65 206
MS.gene013966.t1 AT3G46640 38.750 320 112 8 5 279 15 295 1.65e-52 175
MS.gene013966.t1 AT3G46640 38.750 320 112 8 5 279 15 295 1.65e-52 175
MS.gene013966.t1 AT3G46640 39.423 312 110 8 5 271 15 292 4.20e-52 174
MS.gene013966.t1 AT5G59570 45.933 209 66 4 11 184 28 224 4.18e-45 155
MS.gene013966.t1 AT5G59570 45.933 209 66 4 11 184 28 224 4.18e-45 155
MS.gene013966.t1 AT3G16857 60.000 75 30 0 79 153 155 229 4.38e-24 102
MS.gene013966.t1 AT3G16857 60.000 75 30 0 79 153 225 299 5.09e-24 102
MS.gene013966.t1 AT3G16857 60.000 75 30 0 79 153 225 299 5.66e-24 102
MS.gene013966.t1 AT5G58080 66.129 62 21 0 88 149 191 252 5.45e-22 96.3
MS.gene013966.t1 AT2G01760 63.077 65 24 0 90 154 134 198 2.82e-21 92.4
MS.gene013966.t1 AT2G01760 63.077 65 24 0 90 154 199 263 7.43e-21 92.0
MS.gene013966.t1 AT2G25180 61.538 65 25 0 90 154 194 258 9.03e-21 92.8
MS.gene013966.t1 AT2G01760 63.077 65 24 0 90 154 227 291 1.27e-20 91.7
MS.gene013966.t1 AT4G31920 61.667 60 23 0 90 149 182 241 3.89e-19 87.8
MS.gene013966.t1 AT5G07210 50.667 75 35 1 82 154 221 295 3.16e-17 82.4
MS.gene013966.t1 AT5G07210 50.667 75 35 1 82 154 213 287 3.41e-17 82.4
MS.gene013966.t1 AT3G62670 38.710 124 66 4 90 205 210 331 4.49e-16 78.6
MS.gene013966.t1 AT5G44190 55.000 60 27 0 90 149 146 205 5.22e-16 78.2
MS.gene013966.t1 AT5G44190 55.000 60 27 0 90 149 146 205 6.09e-16 77.8
MS.gene013966.t1 AT2G20570 55.000 60 27 0 90 149 152 211 3.67e-15 75.9
MS.gene013966.t1 AT2G20570 57.895 57 24 0 93 149 171 227 9.08e-15 74.7
MS.gene013966.t1 AT1G67710 59.322 59 23 1 93 150 121 179 1.98e-14 73.6
MS.gene013966.t1 AT1G67710 59.322 59 23 1 93 150 130 188 2.10e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.51e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.51e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.51e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.51e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.51e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.55e-14 73.6
MS.gene013966.t1 AT3G12730 61.818 55 20 1 91 144 23 77 2.91e-14 71.2
MS.gene013966.t1 AT1G67710 59.322 59 23 1 93 150 195 253 2.91e-14 73.2
MS.gene013966.t1 AT2G27070 48.529 68 35 0 82 149 216 283 3.37e-14 73.2
MS.gene013966.t1 AT3G04030 57.407 54 23 0 91 144 45 98 9.48e-14 71.6
MS.gene013966.t1 AT5G45580 58.182 55 22 1 91 144 7 61 2.37e-13 69.3
MS.gene013966.t1 AT1G79430 61.818 55 20 1 91 144 34 88 2.58e-13 70.1
MS.gene013966.t1 AT5G45580 58.182 55 22 1 91 144 23 77 2.76e-13 69.3
MS.gene013966.t1 AT2G03500 53.333 60 27 1 90 148 232 291 3.27e-13 70.1
MS.gene013966.t1 AT3G04030 58.182 55 22 1 91 144 45 99 4.76e-13 69.3
MS.gene013966.t1 AT3G04030 58.182 55 22 1 91 144 45 99 5.23e-13 69.3
MS.gene013966.t1 AT3G24120 60.000 55 21 1 91 144 41 95 5.99e-13 68.6
MS.gene013966.t1 AT3G24120 60.000 55 21 1 91 144 41 95 6.30e-13 68.2
MS.gene013966.t1 AT4G13640 60.000 55 21 1 91 144 37 91 6.73e-13 68.2
MS.gene013966.t1 AT4G13640 60.000 55 21 1 91 144 37 91 7.57e-13 68.2
MS.gene013966.t1 AT5G18240 60.000 55 21 1 91 144 45 99 9.35e-13 68.6
MS.gene013966.t1 AT5G18240 60.000 55 21 1 91 144 45 99 9.35e-13 68.6
MS.gene013966.t1 AT5G49240 52.727 55 26 0 86 140 218 272 9.70e-13 67.8
MS.gene013966.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.03e-12 68.6
MS.gene013966.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.03e-12 68.6
MS.gene013966.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.03e-12 68.6
MS.gene013966.t1 AT1G13300 51.667 60 28 1 90 148 180 239 1.16e-12 67.8
MS.gene013966.t1 AT1G25550 51.667 60 28 1 90 148 208 267 2.24e-12 67.0
MS.gene013966.t1 AT1G49190 32.692 104 62 4 90 187 214 315 8.85e-12 65.5
MS.gene013966.t1 AT2G01060 58.182 55 22 1 91 144 15 69 1.21e-11 64.3
MS.gene013966.t1 AT1G68670 50.000 62 30 1 88 148 212 273 1.94e-11 64.3
MS.gene013966.t1 AT4G37180 50.000 60 29 1 90 148 209 268 3.43e-11 63.5
MS.gene013966.t1 AT1G49560 48.387 62 31 1 88 148 189 250 3.81e-11 63.5
MS.gene013966.t1 AT4G37180 50.000 60 29 1 90 148 216 275 4.32e-11 63.5
MS.gene013966.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.34e-11 63.5
MS.gene013966.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.34e-11 63.5
MS.gene013966.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.34e-11 63.5

Find 97 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGTCCGGTTGGACATTT+TGG 0.084147 7.2:+19484420 MS.gene013966:CDS
GGGATAGTGGTGGGTGGATT+AGG 0.144441 7.2:-19483904 None:intergenic
CAGCAGCAGCTTCATAGAAT+TGG 0.261093 7.2:+19484528 MS.gene013966:CDS
TGGTGGTGGTGTGGAGGATA+TGG 0.268130 7.2:+19484596 MS.gene013966:CDS
GTGATTAGGGTTTGTGATAA+AGG 0.272510 7.2:-19483844 None:intergenic
GGATAGTGGTGGGTGGATTA+GGG 0.278062 7.2:-19483903 None:intergenic
TGAGAGAAGATGATTCAAAT+TGG 0.306698 7.2:+19483776 MS.gene013966:CDS
TGGCGAGATCAGGTGTGATT+AGG 0.327778 7.2:-19483858 None:intergenic
TTCAGTTGAATCGGCGAAAT+CGG 0.335433 7.2:-19483977 None:intergenic
TCCTGTTGCGCGGCCGAATT+CGG 0.336857 7.2:+19484335 MS.gene013966:CDS
AGCAGCAGCTTCATAGAATT+GGG 0.346797 7.2:+19484529 MS.gene013966:CDS
CGCATGCAGGGTATTTCTTC+CGG 0.354356 7.2:+19484216 MS.gene013966:CDS
GGCGAGATCAGGTGTGATTA+GGG 0.364114 7.2:-19483857 None:intergenic
GTGGAGGAAGGAAGAGAAGA+AGG 0.372281 7.2:-19483955 None:intergenic
CGGCGTCGACGAAGCGTTTG+TGG 0.387001 7.2:-19484074 None:intergenic
TTGTTGTGGTGGTGGGATAG+TGG 0.396749 7.2:-19483917 None:intergenic
CAATTCTATGAAGCTGCTGC+TGG 0.402280 7.2:-19484527 None:intergenic
GGTGGTGGTGGTTCCGGTGG+TGG 0.402979 7.2:+19484240 MS.gene013966:CDS
TCCGAATTCGGCCGCGCAAC+AGG 0.403622 7.2:-19484336 None:intergenic
GATAAAGGCATGAGTTCTTC+TGG 0.404291 7.2:-19483829 None:intergenic
TGGTTCTTCGCCGGCGGTGC+CGG 0.408437 7.2:-19484007 None:intergenic
TGAAGAAAATGCGGCGGTAC+AGG 0.411146 7.2:-19484312 None:intergenic
GGCATAAAATGATCCGAATT+CGG 0.417026 7.2:-19484348 None:intergenic
ACCGGAACCACCACCACCAC+CGG 0.421482 7.2:-19484235 None:intergenic
TATGAAGCTGCTGCTGGTGC+TGG 0.424890 7.2:-19484521 None:intergenic
TCGAAAGCGATGGCGAGATC+AGG 0.427672 7.2:-19483868 None:intergenic
ACTTTAGGTGGTGGTGGTGG+TGG 0.427931 7.2:+19484570 MS.gene013966:CDS
GTTGTTGTTGTTGTGGTGGT+GGG 0.428602 7.2:-19483924 None:intergenic
TGGAGGAAGGAAGAGAAGAA+GGG 0.433177 7.2:-19483954 None:intergenic
CAGGTGCATCATAACACTTT+AGG 0.433744 7.2:+19484555 MS.gene013966:CDS
CATAAGCTGCATTATCGTCT+TGG 0.434900 7.2:-19484127 None:intergenic
ATTTCTTCCGGTGGTGGTGG+TGG 0.438743 7.2:+19484228 MS.gene013966:CDS
TTGTGGTGGTGGGATAGTGG+TGG 0.448450 7.2:-19483914 None:intergenic
TCAAACGCCCTCGTCTTGTA+TGG 0.450475 7.2:+19484040 MS.gene013966:CDS
TCTTTATCTTAAACGCATGC+AGG 0.456959 7.2:+19484203 MS.gene013966:CDS
CAGGGTATTTCTTCCGGTGG+TGG 0.460767 7.2:+19484222 MS.gene013966:CDS
GGTATTTCTTCCGGTGGTGG+TGG 0.460989 7.2:+19484225 MS.gene013966:CDS
CGAAGCGTTTGTGGAGCTGT+GGG 0.467151 7.2:-19484065 None:intergenic
GTTCTGACTTTGTTTCCAAC+CGG 0.469745 7.2:+19484648 MS.gene013966:CDS
GTCGACGCCGTGGCGCATCT+CGG 0.482986 7.2:+19484087 MS.gene013966:CDS
ATGGAATCAGCTAATGCAAC+CGG 0.487862 7.2:+19484615 MS.gene013966:CDS
ATAAGCTGCATTATCGTCTT+GGG 0.488899 7.2:-19484126 None:intergenic
AACCCAAATTCAATCATCTC+CGG 0.488992 7.2:-19484667 None:intergenic
TGATGATGAATAGCAGGAAC+AGG 0.492936 7.2:-19484378 None:intergenic
AACACTTTAGGTGGTGGTGG+TGG 0.493430 7.2:+19484567 MS.gene013966:CDS
GGATTATCAAAATGTCCATT+CGG 0.494489 7.2:-19484450 None:intergenic
GAGGAAGGAAGAGAAGAAGG+GGG 0.497445 7.2:-19483952 None:intergenic
GGCATGAGTTCTTCTGGTGG+TGG 0.507634 7.2:-19483823 None:intergenic
GGCGACCCAAAATGTCCAAC+CGG 0.507671 7.2:-19484426 None:intergenic
TGCTGCTGATGATGAATAGC+AGG 0.508146 7.2:-19484384 None:intergenic
TAATGCAACCGGAGGGAGAA+AGG 0.508295 7.2:+19484626 MS.gene013966:CDS
GGAGGTGAGTTCAGTTGAAT+CGG 0.513901 7.2:-19483986 None:intergenic
GGAGGAAGGAAGAGAAGAAG+GGG 0.527059 7.2:-19483953 None:intergenic
AGTCAGAACCTTTCTCCCTC+CGG 0.527350 7.2:-19484634 None:intergenic
ACGAAGCGTTTGTGGAGCTG+TGG 0.532297 7.2:-19484066 None:intergenic
TGAATCGGCGAAATCGGTGG+AGG 0.543830 7.2:-19483971 None:intergenic
TTCTTCGCCGGCGGTGCCGG+AGG 0.545793 7.2:-19484004 None:intergenic
GAACTCACCTCCGGCACCGC+CGG 0.547744 7.2:+19483997 MS.gene013966:CDS
TGTGGTGGTGGGATAGTGGT+GGG 0.548736 7.2:-19483913 None:intergenic
GCTTCATAGAATTGGGGCAC+AGG 0.550758 7.2:+19484536 MS.gene013966:CDS
CATAACACTTTAGGTGGTGG+TGG 0.551977 7.2:+19484564 MS.gene013966:CDS
GATGGCTCAGTATCATCGTC+CGG 0.554969 7.2:+19484407 MS.gene013966:CDS
AGTTGAATCGGCGAAATCGG+TGG 0.557119 7.2:-19483974 None:intergenic
GAAGAAAATGCGGCGGTACA+GGG 0.558776 7.2:-19484311 None:intergenic
GGGCGTTTGAGTGTGCGAGC+TGG 0.560084 7.2:-19484027 None:intergenic
CTTTATCTTAAACGCATGCA+GGG 0.564649 7.2:+19484204 MS.gene013966:CDS
TGTGCGAGCTGGTTCTTCGC+CGG 0.569228 7.2:-19484016 None:intergenic
TCGGCGAAATCGGTGGAGGA+AGG 0.569986 7.2:-19483967 None:intergenic
ATAGCAGGAACAGGAACAAA+CGG 0.570737 7.2:-19484369 None:intergenic
CATCATAACACTTTAGGTGG+TGG 0.574928 7.2:+19484561 MS.gene013966:CDS
AACAGAAGCAGAACCACCAC+CGG 0.574953 7.2:-19484253 None:intergenic
GCATGAGTTCTTCTGGTGGT+GGG 0.580278 7.2:-19483822 None:intergenic
GCTCAGTATCATCGTCCGGT+TGG 0.583168 7.2:+19484411 MS.gene013966:CDS
GTTTCGTATGTCGAAAGCGA+TGG 0.584109 7.2:-19483878 None:intergenic
AAGAAAATGCGGCGGTACAG+GGG 0.587168 7.2:-19484310 None:intergenic
ATGCAGGGTATTTCTTCCGG+TGG 0.591915 7.2:+19484219 MS.gene013966:CDS
CAAATTCAATCATCTCCGGT+TGG 0.598529 7.2:-19484663 None:intergenic
ATGCAGCTTATGAGTGTGGA+TGG 0.599001 7.2:+19484138 MS.gene013966:CDS
GGTGGTGGGATAGTGGTGGG+TGG 0.599530 7.2:-19483910 None:intergenic
TATTCATCATCAGCAGCAGA+TGG 0.603490 7.2:+19484389 MS.gene013966:CDS
GCGAGCTGGTTCTTCGCCGG+CGG 0.605171 7.2:-19484013 None:intergenic
CTTGATACCGAGATGCGCCA+CGG 0.632900 7.2:-19484094 None:intergenic
GAATCAGCTAATGCAACCGG+AGG 0.639788 7.2:+19484618 MS.gene013966:CDS
GATTCAACTGAACTCACCTC+CGG 0.640353 7.2:+19483988 MS.gene013966:CDS
TGGTTTAACGAGAGAAAACG+TGG 0.642801 7.2:+19484158 MS.gene013966:CDS
GCAGCAGCTTCATAGAATTG+GGG 0.666624 7.2:+19484530 MS.gene013966:CDS
GTGCATCATAACACTTTAGG+TGG 0.672948 7.2:+19484558 MS.gene013966:CDS
TGTGGGGTCCATACAAGACG+AGG 0.673250 7.2:-19484048 None:intergenic
CAAACGCTTCGTCGACGCCG+TGG 0.674680 7.2:+19484077 MS.gene013966:CDS
GATAATGCAGCTTATGAGTG+TGG 0.675001 7.2:+19484134 MS.gene013966:CDS
GCAACAGGATGAAGAAAATG+CGG 0.684941 7.2:-19484321 None:intergenic
GAAGCGTTTGTGGAGCTGTG+GGG 0.695777 7.2:-19484064 None:intergenic
TTATCAAAATGTCCATTCGG+CGG 0.700387 7.2:-19484447 None:intergenic
AATCAGCTAATGCAACCGGA+GGG 0.707357 7.2:+19484619 MS.gene013966:CDS
GTGGGGTCCATACAAGACGA+GGG 0.708697 7.2:-19484047 None:intergenic
ACAGGATGAAGAAAATGCGG+CGG 0.721657 7.2:-19484318 None:intergenic
AAAGGCATGAGTTCTTCTGG+TGG 0.761512 7.2:-19483826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAAATTGGTTTTCAAAA+TGG + chr7.2:19483788-19483807 MS.gene013966:CDS 20.0%
!!! TTCAAATTGGTTTTCAAAAT+GGG + chr7.2:19483789-19483808 MS.gene013966:CDS 20.0%
GGATTATCAAAATGTCCATT+CGG - chr7.2:19484453-19484472 None:intergenic 30.0%
TGAGAGAAGATGATTCAAAT+TGG + chr7.2:19483776-19483795 MS.gene013966:CDS 30.0%
ATAAGCTGCATTATCGTCTT+GGG - chr7.2:19484129-19484148 None:intergenic 35.0%
CTTTATCTTAAACGCATGCA+GGG + chr7.2:19484204-19484223 MS.gene013966:CDS 35.0%
GGCATAAAATGATCCGAATT+CGG - chr7.2:19484351-19484370 None:intergenic 35.0%
GTGATTAGGGTTTGTGATAA+AGG - chr7.2:19483847-19483866 None:intergenic 35.0%
TCTTTATCTTAAACGCATGC+AGG + chr7.2:19484203-19484222 MS.gene013966:CDS 35.0%
TGTTGCTGTCTAGACAAAAA+AGG - chr7.2:19484474-19484493 None:intergenic 35.0%
TTATCAAAATGTCCATTCGG+CGG - chr7.2:19484450-19484469 None:intergenic 35.0%
AGCAGCAGCTTCATAGAATT+GGG + chr7.2:19484529-19484548 MS.gene013966:CDS 40.0%
ATAGCAGGAACAGGAACAAA+CGG - chr7.2:19484372-19484391 None:intergenic 40.0%
ATGGAATCAGCTAATGCAAC+CGG + chr7.2:19484615-19484634 MS.gene013966:CDS 40.0%
CAGGTGCATCATAACACTTT+AGG + chr7.2:19484555-19484574 MS.gene013966:CDS 40.0%
CATAAGCTGCATTATCGTCT+TGG - chr7.2:19484130-19484149 None:intergenic 40.0%
CATCATAACACTTTAGGTGG+TGG + chr7.2:19484561-19484580 MS.gene013966:CDS 40.0%
GATAATGCAGCTTATGAGTG+TGG + chr7.2:19484134-19484153 MS.gene013966:CDS 40.0%
GCAACAGGATGAAGAAAATG+CGG - chr7.2:19484324-19484343 None:intergenic 40.0%
GTGCATCATAACACTTTAGG+TGG + chr7.2:19484558-19484577 MS.gene013966:CDS 40.0%
TATTCATCATCAGCAGCAGA+TGG + chr7.2:19484389-19484408 MS.gene013966:CDS 40.0%
TGGTTTAACGAGAGAAAACG+TGG + chr7.2:19484158-19484177 MS.gene013966:CDS 40.0%
TTCAGTTGAATCGGCGAAAT+CGG - chr7.2:19483980-19483999 None:intergenic 40.0%
! ATTTTCTTCATCCTGTTGCG+CGG + chr7.2:19484325-19484344 MS.gene013966:CDS 40.0%
! GATAAAGGCATGAGTTCTTC+TGG - chr7.2:19483832-19483851 None:intergenic 40.0%
! GTTCTGACTTTGTTTCCAAC+CGG + chr7.2:19484648-19484667 MS.gene013966:CDS 40.0%
! TGATGATGAATAGCAGGAAC+AGG - chr7.2:19484381-19484400 None:intergenic 40.0%
AATCAGCTAATGCAACCGGA+GGG + chr7.2:19484619-19484638 MS.gene013966:CDS 45.0%
ACAGGATGAAGAAAATGCGG+CGG - chr7.2:19484321-19484340 None:intergenic 45.0%
ATGCAGCTTATGAGTGTGGA+TGG + chr7.2:19484138-19484157 MS.gene013966:CDS 45.0%
CAATTCTATGAAGCTGCTGC+TGG - chr7.2:19484530-19484549 None:intergenic 45.0%
CAGCAGCAGCTTCATAGAAT+TGG + chr7.2:19484528-19484547 MS.gene013966:CDS 45.0%
GATTCAACTGAACTCACCTC+CGG + chr7.2:19483988-19484007 MS.gene013966:CDS 45.0%
GCAGCAGCTTCATAGAATTG+GGG + chr7.2:19484530-19484549 MS.gene013966:CDS 45.0%
GGAGGTGAGTTCAGTTGAAT+CGG - chr7.2:19483989-19484008 None:intergenic 45.0%
GTTTCGTATGTCGAAAGCGA+TGG - chr7.2:19483881-19483900 None:intergenic 45.0%
TGGAGGAAGGAAGAGAAGAA+GGG - chr7.2:19483957-19483976 None:intergenic 45.0%
! AAAGGCATGAGTTCTTCTGG+TGG - chr7.2:19483829-19483848 None:intergenic 45.0%
! ATCGTCCGGTTGGACATTTT+GGG + chr7.2:19484421-19484440 MS.gene013966:CDS 45.0%
! CATAACACTTTAGGTGGTGG+TGG + chr7.2:19484564-19484583 MS.gene013966:CDS 45.0%
! GGTGGTTGTTGTTGTTGTTG+TGG - chr7.2:19483934-19483953 None:intergenic 45.0%
! GGTTGTTGTTGTTGTTGTGG+TGG - chr7.2:19483931-19483950 None:intergenic 45.0%
! GTTGTTGTTGTTGTGGTGGT+GGG - chr7.2:19483927-19483946 None:intergenic 45.0%
! TGCTGCTGATGATGAATAGC+AGG - chr7.2:19484387-19484406 None:intergenic 45.0%
! TGTTGTTGTTGTTGTGGTGG+TGG - chr7.2:19483928-19483947 None:intergenic 45.0%
AACAGAAGCAGAACCACCAC+CGG - chr7.2:19484256-19484275 None:intergenic 50.0%
AAGAAAATGCGGCGGTACAG+GGG - chr7.2:19484313-19484332 None:intergenic 50.0%
AGTCAGAACCTTTCTCCCTC+CGG - chr7.2:19484637-19484656 None:intergenic 50.0%
AGTTGAATCGGCGAAATCGG+TGG - chr7.2:19483977-19483996 None:intergenic 50.0%
GAAGAAAATGCGGCGGTACA+GGG - chr7.2:19484314-19484333 None:intergenic 50.0%
GAATCAGCTAATGCAACCGG+AGG + chr7.2:19484618-19484637 MS.gene013966:CDS 50.0%
GAGGAAGGAAGAGAAGAAGG+GGG - chr7.2:19483955-19483974 None:intergenic 50.0%
GATGGCTCAGTATCATCGTC+CGG + chr7.2:19484407-19484426 MS.gene013966:CDS 50.0%
GCATGAGTTCTTCTGGTGGT+GGG - chr7.2:19483825-19483844 None:intergenic 50.0%
GCTTCATAGAATTGGGGCAC+AGG + chr7.2:19484536-19484555 MS.gene013966:CDS 50.0%
GGAGGAAGGAAGAGAAGAAG+GGG - chr7.2:19483956-19483975 None:intergenic 50.0%
GGCGAGATCAGGTGTGATTA+GGG - chr7.2:19483860-19483879 None:intergenic 50.0%
GTGGAGGAAGGAAGAGAAGA+AGG - chr7.2:19483958-19483977 None:intergenic 50.0%
TAATGCAACCGGAGGGAGAA+AGG + chr7.2:19484626-19484645 MS.gene013966:CDS 50.0%
TCAAACGCCCTCGTCTTGTA+TGG + chr7.2:19484040-19484059 MS.gene013966:CDS 50.0%
TGAAGAAAATGCGGCGGTAC+AGG - chr7.2:19484315-19484334 None:intergenic 50.0%
TGGCGAGATCAGGTGTGATT+AGG - chr7.2:19483861-19483880 None:intergenic 50.0%
! ATGCAGGGTATTTCTTCCGG+TGG + chr7.2:19484219-19484238 MS.gene013966:CDS 50.0%
! CATCGTCCGGTTGGACATTT+TGG + chr7.2:19484420-19484439 MS.gene013966:CDS 50.0%
! CGCATGCAGGGTATTTCTTC+CGG + chr7.2:19484216-19484235 MS.gene013966:CDS 50.0%
!! AACACTTTAGGTGGTGGTGG+TGG + chr7.2:19484567-19484586 MS.gene013966:CDS 50.0%
!! GGATAGTGGTGGGTGGATTA+GGG - chr7.2:19483906-19483925 None:intergenic 50.0%
!! TTGTTGTGGTGGTGGGATAG+TGG - chr7.2:19483920-19483939 None:intergenic 50.0%
CTTGATACCGAGATGCGCCA+CGG - chr7.2:19484097-19484116 None:intergenic 55.0%
GAAGGAAGAGAAGAAGGGGG+TGG - chr7.2:19483952-19483971 None:intergenic 55.0%
GCTCAGTATCATCGTCCGGT+TGG + chr7.2:19484411-19484430 MS.gene013966:CDS 55.0%
GGCATGAGTTCTTCTGGTGG+TGG - chr7.2:19483826-19483845 None:intergenic 55.0%
GGCGACCCAAAATGTCCAAC+CGG - chr7.2:19484429-19484448 None:intergenic 55.0%
GTGGGGTCCATACAAGACGA+GGG - chr7.2:19484050-19484069 None:intergenic 55.0%
TATGAAGCTGCTGCTGGTGC+TGG - chr7.2:19484524-19484543 None:intergenic 55.0%
TCGAAAGCGATGGCGAGATC+AGG - chr7.2:19483871-19483890 None:intergenic 55.0%
TGAATCGGCGAAATCGGTGG+AGG - chr7.2:19483974-19483993 None:intergenic 55.0%
TGTGGGGTCCATACAAGACG+AGG - chr7.2:19484051-19484070 None:intergenic 55.0%
! ACGAAGCGTTTGTGGAGCTG+TGG - chr7.2:19484069-19484088 None:intergenic 55.0%
! CAGGGTATTTCTTCCGGTGG+TGG + chr7.2:19484222-19484241 MS.gene013966:CDS 55.0%
! CGAAGCGTTTGTGGAGCTGT+GGG - chr7.2:19484068-19484087 None:intergenic 55.0%
! GAAGCGTTTGTGGAGCTGTG+GGG - chr7.2:19484067-19484086 None:intergenic 55.0%
! GGTATTTCTTCCGGTGGTGG+TGG + chr7.2:19484225-19484244 MS.gene013966:CDS 55.0%
!! ACTTTAGGTGGTGGTGGTGG+TGG + chr7.2:19484570-19484589 MS.gene013966:CDS 55.0%
!! ATTTCTTCCGGTGGTGGTGG+TGG + chr7.2:19484228-19484247 MS.gene013966:CDS 55.0%
!! GGGATAGTGGTGGGTGGATT+AGG - chr7.2:19483907-19483926 None:intergenic 55.0%
!! TGGTGGTGGTGTGGAGGATA+TGG + chr7.2:19484596-19484615 MS.gene013966:CDS 55.0%
!! TGTGGTGGTGGGATAGTGGT+GGG - chr7.2:19483916-19483935 None:intergenic 55.0%
!! TTGTGGTGGTGGGATAGTGG+TGG - chr7.2:19483917-19483936 None:intergenic 55.0%
TCCTGTTGCGCGGCCGAATT+CGG + chr7.2:19484335-19484354 MS.gene013966:CDS 60.0%
TCGGCGAAATCGGTGGAGGA+AGG - chr7.2:19483970-19483989 None:intergenic 60.0%
! TGTGCGAGCTGGTTCTTCGC+CGG - chr7.2:19484019-19484038 None:intergenic 60.0%
!! CATTTTGGGTCGCCGCCGAA+TGG + chr7.2:19484435-19484454 MS.gene013966:CDS 60.0%
!! TTAGGTGGTGGTGGTGGTGG+TGG + chr7.2:19484573-19484592 MS.gene013966:CDS 60.0%
ACCGGAACCACCACCACCAC+CGG - chr7.2:19484238-19484257 None:intergenic 65.0%
CAAACGCTTCGTCGACGCCG+TGG + chr7.2:19484077-19484096 MS.gene013966:CDS 65.0%
GGGCGTTTGAGTGTGCGAGC+TGG - chr7.2:19484030-19484049 None:intergenic 65.0%
TCCGAATTCGGCCGCGCAAC+AGG - chr7.2:19484339-19484358 None:intergenic 65.0%
!! CGGCGTCGACGAAGCGTTTG+TGG - chr7.2:19484077-19484096 None:intergenic 65.0%
!! GGTGGTGGGATAGTGGTGGG+TGG - chr7.2:19483913-19483932 None:intergenic 65.0%
!! TCCGGTGGTGGTGGTGGTTC+CGG + chr7.2:19484234-19484253 MS.gene013966:CDS 65.0%
!! TGGTGGTGGTGGTGGTGGTG+TGG + chr7.2:19484578-19484597 MS.gene013966:CDS 65.0%
!! TGGTGGTGGTGGTGGTGTGG+AGG + chr7.2:19484590-19484609 MS.gene013966:CDS 65.0%
GAACTCACCTCCGGCACCGC+CGG + chr7.2:19483997-19484016 MS.gene013966:CDS 70.0%
GTCGACGCCGTGGCGCATCT+CGG + chr7.2:19484087-19484106 MS.gene013966:CDS 70.0%
TGGTTCTTCGCCGGCGGTGC+CGG - chr7.2:19484010-19484029 None:intergenic 70.0%
! GCGAGCTGGTTCTTCGCCGG+CGG - chr7.2:19484016-19484035 None:intergenic 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG + chr7.2:19484576-19484595 MS.gene013966:CDS 70.0%
!! GGTGGTGGTGGTGGTTCCGG+TGG + chr7.2:19484237-19484256 MS.gene013966:CDS 70.0%
!! GGTGGTGGTGGTTCCGGTGG+TGG + chr7.2:19484240-19484259 MS.gene013966:CDS 70.0%
! TTCTTCGCCGGCGGTGCCGG+AGG - chr7.2:19484007-19484026 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 19483775 19484680 19483775 ID=MS.gene013966
chr7.2 mRNA 19483775 19484680 19483775 ID=MS.gene013966.t1;Parent=MS.gene013966
chr7.2 exon 19483775 19484680 19483775 ID=MS.gene013966.t1.exon1;Parent=MS.gene013966.t1
chr7.2 CDS 19483775 19484680 19483775 ID=cds.MS.gene013966.t1;Parent=MS.gene013966.t1
Gene Sequence

>MS.gene013966

ATGAGAGAAGATGATTCAAATTGGTTTTCAAAATGGGAAGAACAACTCCCACCACCAGAAGAACTCATGCCTTTATCACAAACCCTAATCACACCTGATCTCGCCATCGCTTTCGACATACGAAACAACCCTAATCCACCCACCACTATCCCACCACCACAACAACAACAACAACCACCCCCTTCTTCTCTTCCTTCCTCCACCGATTTCGCCGATTCAACTGAACTCACCTCCGGCACCGCCGGCGAAGAACCAGCTCGCACACTCAAACGCCCTCGTCTTGTATGGACCCCACAGCTCCACAAACGCTTCGTCGACGCCGTGGCGCATCTCGGTATCAAGAACGCTGTTCCCAAGACGATAATGCAGCTTATGAGTGTGGATGGTTTAACGAGAGAAAACGTGGCGAGTCATCTTCAGAAATATCGTCTTTATCTTAAACGCATGCAGGGTATTTCTTCCGGTGGTGGTGGTGGTTCCGGTGGTGGTTCTGCTTCTGTTAATGATGCTGCTACTGATCATTTATTTGCTAGCTCCCCTGTACCGCCGCATTTTCTTCATCCTGTTGCGCGGCCGAATTCGGATCATTTTATGCCGTTTGTTCCTGTTCCTGCTATTCATCATCAGCAGCAGATGGCTCAGTATCATCGTCCGGTTGGACATTTTGGGTCGCCGCCGAATGGACATTTTGATAATCCTTTTTTGTCTAGACAGCAACATCATCAACAGCAAAATCAGCATCAACACCAGCACCAGCAGCAGCTTCATAGAATTGGGGCACAGGTGCATCATAACACTTTAGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGTGGAGGATATGGAATCAGCTAATGCAACCGGAGGGAGAAAGGTTCTGACTTTGTTTCCAACCGGAGATGATTGA
Protein sequence

>MS.gene013966.t1

MREDDSNWFSKWEEQLPPPEELMPLSQTLITPDLAIAFDIRNNPNPPTTIPPPQQQQQPPPSSLPSSTDFADSTELTSGTAGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGISSGGGGGSGGGSASVNDAATDHLFASSPVPPHFLHPVARPNSDHFMPFVPVPAIHHQQQMAQYHRPVGHFGSPPNGHFDNPFLSRQQHHQQQNQHQHQHQQQLHRIGAQVHHNTLGGGGGGGGGGVEDMESANATGGRKVLTLFPTGDD