AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014011

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014011.t1 MTR_7g090060 94.723 379 20 0 1 379 1 379 0.0 747
MS.gene014011.t1 MTR_6g092520 74.263 373 85 3 6 378 2 363 0.0 556
MS.gene014011.t1 MTR_6g092500 61.062 113 39 1 3 115 46 153 3.49e-42 145
MS.gene014011.t1 MTR_6g092480 44.715 123 42 1 125 247 9 105 5.13e-26 110
MS.gene014011.t1 MTR_6g092480 65.909 44 15 0 270 313 311 354 1.98e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014011.t1 AT5G56780 42.010 388 186 9 1 378 11 369 4.98e-91 282
MS.gene014011.t1 AT5G56770 42.529 174 76 4 2 174 51 201 1.12e-35 131

Find 109 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACAACCTTCCTCAGAAAT+TGG 0.157069 7.2:-18632370 MS.gene014011:CDS
CTACATCATGGCTGGCATTT+TGG 0.209063 7.2:+18630804 None:intergenic
AACGGGAAGGTCATTGTTTC+AGG 0.214002 7.2:-18632148 MS.gene014011:CDS
TTCTCCTCCCTTTATGTTCC+TGG 0.242712 7.2:+18630654 None:intergenic
GGCAGCTTGCTTGATTTAAT+TGG 0.245613 7.2:+18631130 None:intergenic
CCGATGGTTCTATTTGTAAA+AGG 0.246592 7.2:-18630952 MS.gene014011:CDS
AGCTGTGTCGCGTAGTAATC+TGG 0.263388 7.2:-18632322 MS.gene014011:CDS
GCTTCCCAATTGTTTATAGA+TGG 0.289991 7.2:-18632110 MS.gene014011:CDS
TTGGTATCCCCACTTTCCTC+TGG 0.290433 7.2:+18631149 None:intergenic
GATTCCACACATAATGGTTT+TGG 0.295658 7.2:+18630733 None:intergenic
ATTCTCATTCCTTTGTGTTC+AGG 0.303340 7.2:+18630902 None:intergenic
CAAGATGATTCCACACATAA+TGG 0.329184 7.2:+18630727 None:intergenic
TGTAGAAGACAACCGGTTAA+AGG 0.332466 7.2:-18630689 MS.gene014011:CDS
CTTGGGTGATCTGATTGCTT+TGG 0.351144 7.2:+18630865 None:intergenic
GCATTGTAGGTAATGCTTAT+AGG 0.363449 7.2:-18630829 MS.gene014011:CDS
TTCTTATCGGTCCTTCTGAT+TGG 0.365524 7.2:-18632437 MS.gene014011:CDS
TCAGAATTAGAAAGCATTGT+AGG 0.383504 7.2:-18630842 MS.gene014011:CDS
GGTGAAAGGTAGAAGCAATT+TGG 0.393179 7.2:+18631171 None:intergenic
AAAAGGTGCCAGGAACATAA+AGG 0.395561 7.2:-18630662 MS.gene014011:CDS
GGAGCCCATCTATAAACAAT+TGG 0.397811 7.2:+18632105 None:intergenic
AACCGGTTAAAGGAAGAAAA+AGG 0.410668 7.2:-18630679 MS.gene014011:CDS
CATGAGAATGCTAAAATTTG+CGG 0.413022 7.2:-18630986 MS.gene014011:CDS
CGCATAGTTGTGGTTTACCT+TGG 0.418108 7.2:-18632264 MS.gene014011:CDS
GCCTCTGACAGATGACAAAC+TGG 0.430967 7.2:-18631110 MS.gene014011:CDS
CACAACCTTCCTCAGAAATT+GGG 0.434094 7.2:-18632369 MS.gene014011:CDS
CCCGTGTGTTCAAATTAAAC+AGG 0.435752 7.2:-18631054 MS.gene014011:CDS
TCTTATCGGTCCTTCTGATT+GGG 0.443716 7.2:-18632436 MS.gene014011:CDS
GCAGCTGAGATTCTGTTCTC+AGG 0.444652 7.2:+18631308 None:intergenic
GTCACGTCCTAGGATAGTTC+AGG 0.452457 7.2:-18631032 MS.gene014011:CDS
GTGGAGGGTCTTCTACATCA+TGG 0.463412 7.2:+18630792 None:intergenic
CTTCCCAATTGTTTATAGAT+GGG 0.464946 7.2:-18632109 MS.gene014011:CDS
GGGTGATCTGATTGCTTTGG+CGG 0.466767 7.2:+18630868 None:intergenic
AGAGCAAGACTCCAGCGTTA+TGG 0.467691 7.2:-18632225 MS.gene014011:CDS
CCTTCCTCAGAAATTGGGTC+CGG 0.470635 7.2:-18632364 MS.gene014011:CDS
TGACTCTTAACCATTCTTTG+TGG 0.474955 7.2:+18630773 None:intergenic
GGAAGACTATTCCAAAGAAA+AGG 0.474965 7.2:-18632415 MS.gene014011:CDS
AAGGTTGTGAATCCTGTATC+TGG 0.479106 7.2:+18632383 None:intergenic
AGCATTTGAAGAATATCATC+AGG 0.480750 7.2:+18631235 None:intergenic
AGGAAGGATCTACCAGATAC+AGG 0.491164 7.2:-18632395 MS.gene014011:CDS
AATTAAACAGGTCACGTCCT+AGG 0.491462 7.2:-18631042 MS.gene014011:CDS
CATTATGTGTGGAATCATCT+TGG 0.495040 7.2:-18630726 MS.gene014011:CDS
ATGCTCAACAAATTAGCTTC+AGG 0.496009 7.2:-18631217 MS.gene014011:CDS
CTCCAGCGTTATGGCAGGAA+TGG 0.496259 7.2:-18632216 MS.gene014011:CDS
TGGCAAGCTTGTAAATTTCA+CGG 0.499268 7.2:+18632298 None:intergenic
CTTCCTCAGAAATTGGGTCC+GGG 0.499654 7.2:-18632363 MS.gene014011:CDS
CCCACTTTCCTCTGGGTGAA+AGG 0.500019 7.2:+18631157 None:intergenic
AATGCTTTCTAATTCTGACT+TGG 0.501548 7.2:+18630847 None:intergenic
TGGTATCCCCACTTTCCTCT+GGG 0.502512 7.2:+18631150 None:intergenic
ATGCTTTCTAATTCTGACTT+GGG 0.502963 7.2:+18630848 None:intergenic
TCTTAACCATTCTTTGTGGA+GGG 0.504208 7.2:+18630777 None:intergenic
CTCTTAACCATTCTTTGTGG+AGG 0.504735 7.2:+18630776 None:intergenic
ATCCACTTGTAGAAGACAAC+CGG 0.506149 7.2:-18630696 MS.gene014011:CDS
AGAAGACCCTCCACAAAGAA+TGG 0.506283 7.2:-18630783 MS.gene014011:CDS
GAGCCCATCTATAAACAATT+GGG 0.508603 7.2:+18632106 None:intergenic
TTCTATTTGTAAAAGGCCAC+CGG 0.517076 7.2:-18630945 MS.gene014011:CDS
ACCTGTTTAATTTGAACACA+CGG 0.522258 7.2:+18631053 None:intergenic
GTGGTATCCTGAACTATCCT+AGG 0.522643 7.2:+18631025 None:intergenic
GATGACTCTTCTCTACGAAC+GGG 0.523910 7.2:-18632165 MS.gene014011:CDS
AGGGTCTTCTACATCATGGC+TGG 0.525907 7.2:+18630796 None:intergenic
AACCGGTTGTCTTCTACAAG+TGG 0.530917 7.2:+18630694 None:intergenic
TTAGTACATTTGATTATGCA+TGG 0.537544 7.2:-18631285 MS.gene014011:CDS
AAAGGTGCCAGGAACATAAA+GGG 0.538956 7.2:-18630661 MS.gene014011:CDS
ACCTTTCACCCAGAGGAAAG+TGG 0.539145 7.2:-18631158 MS.gene014011:CDS
CAGCTGAGATTCTGTTCTCA+GGG 0.541120 7.2:+18631309 None:intergenic
CCTTTCACCCAGAGGAAAGT+GGG 0.542001 7.2:-18631157 MS.gene014011:CDS
ATGATTCTAGCTTCTCCCAC+TGG 0.543441 7.2:-18633174 MS.gene014011:CDS
ACTCCAAGCTCGTACACCCC+CGG 0.547377 7.2:+18632345 None:intergenic
TTAATTTGAACACACGGGAT+AGG 0.547956 7.2:+18631059 None:intergenic
TGTGGAATCATCTTGGATGA+TGG 0.551166 7.2:-18630719 MS.gene014011:CDS
CAAGACTCCAGCGTTATGGC+AGG 0.554748 7.2:-18632221 MS.gene014011:CDS
CTCCATTCCTGCCATAACGC+TGG 0.555122 7.2:+18632214 None:intergenic
GCTGTGTCGCGTAGTAATCT+GGG 0.557788 7.2:-18632321 MS.gene014011:CDS
TTCCTCAGAAATTGGGTCCG+GGG 0.559029 7.2:-18632362 MS.gene014011:CDS
TAAAATTTGCGGAGTGATGT+TGG 0.559942 7.2:-18630975 MS.gene014011:CDS
GTTAAGAGTCATGTTGATGA+GGG 0.562383 7.2:-18630761 MS.gene014011:CDS
TTCTAGCTTCTCCCACTGGA+AGG 0.570084 7.2:-18633170 MS.gene014011:intron
GAATAGTCTTCCCAATCAGA+AGG 0.574116 7.2:+18632426 None:intergenic
TGACCCTCATCGCATAGTTG+TGG 0.574795 7.2:-18632274 MS.gene014011:CDS
GGTTAAGAGTCATGTTGATG+AGG 0.575589 7.2:-18630762 MS.gene014011:CDS
CCGGACCCAATTTCTGAGGA+AGG 0.577737 7.2:+18632364 None:intergenic
GGTCGTCAACAGATTGAAGC+GGG 0.577925 7.2:-18633218 MS.gene014011:CDS
AGATGACTCTTCTCTACGAA+CGG 0.578063 7.2:-18632166 MS.gene014011:CDS
TCCTCAGAAATTGGGTCCGG+GGG 0.579255 7.2:-18632361 MS.gene014011:CDS
TGCGGAGTGATGTTGGCCGA+TGG 0.582561 7.2:-18630968 MS.gene014011:CDS
CCTGTTTAATTTGAACACAC+GGG 0.590009 7.2:+18631054 None:intergenic
AACTATGCGATGAGGGTCAG+TGG 0.590571 7.2:+18632278 None:intergenic
AGGATAGTTCAGGATACCAC+TGG 0.597133 7.2:-18631022 MS.gene014011:CDS
TGATCTGATTGCTTTGGCGG+CGG 0.601310 7.2:+18630871 None:intergenic
TCCAGTTTGTCATCTGTCAG+AGG 0.601705 7.2:+18631109 None:intergenic
TGCTTCTACCTTTCACCCAG+AGG 0.610412 7.2:-18631165 MS.gene014011:CDS
GACTATTCCAAAGAAAAGGA+AGG 0.610651 7.2:-18632411 MS.gene014011:CDS
ATCGCCAAAACCATTATGTG+TGG 0.621653 7.2:-18630737 MS.gene014011:CDS
AAACCACAACTATGCGATGA+GGG 0.622038 7.2:+18632271 None:intergenic
ACACAATCAGCAATGGTACA+CGG 0.633965 7.2:-18631261 MS.gene014011:CDS
GCATGGAACACAATCAGCAA+TGG 0.645734 7.2:-18631268 MS.gene014011:CDS
TGGTCGTCAACAGATTGAAG+CGG 0.649124 7.2:-18633219 MS.gene014011:CDS
TATTGAACAGATGCAAAACG+AGG 0.650690 7.2:-18631338 MS.gene014011:intron
AATTGATATGAACCTTCCAG+TGG 0.655241 7.2:+18633158 None:intergenic
ATTGATATGAACCTTCCAGT+GGG 0.662855 7.2:+18633159 None:intergenic
ATGAGTGGCACTACCACCAG+TGG 0.671537 7.2:+18631006 None:intergenic
ACTCTTCTCTACGAACGGGA+AGG 0.673197 7.2:-18632161 MS.gene014011:CDS
TAAACCACAACTATGCGATG+AGG 0.681351 7.2:+18632270 None:intergenic
CTTTCACCCAGAGGAAAGTG+GGG 0.681368 7.2:-18631156 MS.gene014011:CDS
ATTGAACAGATGCAAAACGA+GGG 0.682609 7.2:-18631337 MS.gene014011:intron
ATAGTTCAGGATACCACTGG+TGG 0.705856 7.2:-18631019 MS.gene014011:CDS
GTGAGGTGTCCTGAACACAA+AGG 0.719035 7.2:-18630911 MS.gene014011:CDS
GGTGCCAGGAACATAAAGGG+AGG 0.732457 7.2:-18630658 MS.gene014011:CDS
AAACTGGATGAAGCTGACAG+TGG 0.772229 7.2:-18631094 MS.gene014011:CDS
CAATCAGCAATGGTACACGG+AGG 0.803408 7.2:-18631258 MS.gene014011:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTTATTTGGTTAT+TGG - chr7.2:18631022-18631041 MS.gene014011:CDS 10.0%
!!! AAATTGAAAACTTTAAAAAG+TGG + chr7.2:18630910-18630929 None:intergenic 15.0%
!!! GAACAAGATATTTTTTTATT+TGG - chr7.2:18631015-18631034 MS.gene014011:CDS 15.0%
!!! TATATAAAAGTGCAATTTTT+TGG - chr7.2:18632459-18632478 MS.gene014011:intron 15.0%
!! TTGTTAACTTCACTATAATA+TGG + chr7.2:18631265-18631284 None:intergenic 20.0%
!!! TTCCATTTAGATAATTTTCT+GGG - chr7.2:18630881-18630900 MS.gene014011:CDS 20.0%
! AAGTGACACAATCAAAATTT+AGG - chr7.2:18632264-18632283 MS.gene014011:CDS 25.0%
! AATATCAGATATGTACTTAC+AGG + chr7.2:18631770-18631789 None:intergenic 25.0%
! ACCTAAATTACATAGATTTC+AGG + chr7.2:18632030-18632049 None:intergenic 25.0%
! ACGTTATCATATGATAGTAT+AGG - chr7.2:18631922-18631941 MS.gene014011:intron 25.0%
! CAAAATCTACTGTTAATATG+TGG - chr7.2:18632383-18632402 MS.gene014011:CDS 25.0%
! CAGAAAATTATCTAAATGGA+AGG + chr7.2:18630882-18630901 None:intergenic 25.0%
! CGTTATCATATGATAGTATA+GGG - chr7.2:18631923-18631942 MS.gene014011:intron 25.0%
! GAATTATCATAATAGACTAG+TGG + chr7.2:18632327-18632346 None:intergenic 25.0%
! GTTTCAAAAAAGTCATTGAT+TGG - chr7.2:18631893-18631912 MS.gene014011:intron 25.0%
! TTAGTACATTTGATTATGCA+TGG - chr7.2:18632566-18632585 MS.gene014011:intron 25.0%
!! AAAAGCTATTGATTGTATGA+TGG - chr7.2:18630942-18630961 MS.gene014011:CDS 25.0%
!! AACCCAATTCCATTTTAAAT+GGG - chr7.2:18632171-18632190 MS.gene014011:CDS 25.0%
!! AACTGTTTTCAGATTCTTAT+CGG - chr7.2:18631401-18631420 MS.gene014011:intron 25.0%
!! CACTAAAAGAATGAAGTATT+AGG + chr7.2:18630838-18630857 None:intergenic 25.0%
!! CTTCCATTTAGATAATTTTC+TGG - chr7.2:18630880-18630899 MS.gene014011:CDS 25.0%
!! TAACCCAATTCCATTTTAAA+TGG - chr7.2:18632170-18632189 MS.gene014011:CDS 25.0%
!! TAGTTTTCGTCATTCTATTA+TGG + chr7.2:18631321-18631340 None:intergenic 25.0%
!!! AATTTTGATTGTGTCACTTT+TGG + chr7.2:18632263-18632282 None:intergenic 25.0%
!!! GCAATTTTTTGGAACTATAA+TGG - chr7.2:18632470-18632489 MS.gene014011:intron 25.0%
AAAACTGGACCCATTTAAAA+TGG + chr7.2:18632183-18632202 None:intergenic 30.0%
AATGCTTTCTAATTCTGACT+TGG + chr7.2:18633007-18633026 None:intergenic 30.0%
AATTAGCAGTGCTCATTTAA+AGG - chr7.2:18632004-18632023 MS.gene014011:intron 30.0%
AATTCATAGCTTAAGAATGG+AGG + chr7.2:18631988-18632007 None:intergenic 30.0%
AGCATTTGAAGAATATCATC+AGG + chr7.2:18632619-18632638 None:intergenic 30.0%
AGCCCAGAAAATTATCTAAA+TGG + chr7.2:18630886-18630905 None:intergenic 30.0%
ATGCTTTCTAATTCTGACTT+GGG + chr7.2:18633006-18633025 None:intergenic 30.0%
CATGAGAATGCTAAAATTTG+CGG - chr7.2:18632865-18632884 MS.gene014011:intron 30.0%
CTCAAATAAACACACATACA+CGG - chr7.2:18631063-18631082 MS.gene014011:CDS 30.0%
CTTCCCAATTGTTTATAGAT+GGG - chr7.2:18631742-18631761 MS.gene014011:intron 30.0%
GCAACTGAACATGTATTAAT+GGG - chr7.2:18631824-18631843 MS.gene014011:intron 30.0%
GCCTGAAATCTATGTAATTT+AGG - chr7.2:18632026-18632045 MS.gene014011:intron 30.0%
GCTAATTCATAGCTTAAGAA+TGG + chr7.2:18631991-18632010 None:intergenic 30.0%
TAGTATAGGGTATAACTCAA+AGG - chr7.2:18631936-18631955 MS.gene014011:intron 30.0%
TCAAATAAACACACATACAC+GGG - chr7.2:18631064-18631083 MS.gene014011:CDS 30.0%
TGCAACTGAACATGTATTAA+TGG - chr7.2:18631823-18631842 MS.gene014011:intron 30.0%
TTCTTTCAACTGTACAAATC+TGG - chr7.2:18631342-18631361 MS.gene014011:intron 30.0%
! AGAATGAAGTATTAGGCTTT+AGG + chr7.2:18630831-18630850 None:intergenic 30.0%
! CAAAATTTAGGACAAGGTTA+GGG - chr7.2:18632276-18632295 MS.gene014011:CDS 30.0%
! CACAATCAAAATTTAGGACA+AGG - chr7.2:18632270-18632289 MS.gene014011:CDS 30.0%
! CCTTTTACAAATAGAACCAT+CGG + chr7.2:18632902-18632921 None:intergenic 30.0%
! GACCCATTTAAAATGGAATT+GGG + chr7.2:18632176-18632195 None:intergenic 30.0%
! TCAAAATTTAGGACAAGGTT+AGG - chr7.2:18632275-18632294 MS.gene014011:CDS 30.0%
! TTAATGTTTCTACGAATCTG+AGG - chr7.2:18630765-18630784 MS.gene014011:CDS 30.0%
! TTTCAGGAGATATTTTCACA+AGG - chr7.2:18631719-18631738 MS.gene014011:intron 30.0%
!! AATTTTAGCATTCTCATGAG+TGG + chr7.2:18632863-18632882 None:intergenic 30.0%
!! ACCTGTTTAATTTGAACACA+CGG + chr7.2:18632801-18632820 None:intergenic 30.0%
!! AGCTAGAATCATGTTTTGTT+CGG + chr7.2:18630669-18630688 None:intergenic 30.0%
!! TCAGAATTAGAAAGCATTGT+AGG - chr7.2:18633009-18633028 MS.gene014011:intron 30.0%
!! TGAACATGTATTAATGGGTT+CGG - chr7.2:18631829-18631848 MS.gene014011:intron 30.0%
!! TTCAGTTCTAGAGATATGTT+AGG - chr7.2:18632235-18632254 MS.gene014011:CDS 30.0%
AACCGGTTAAAGGAAGAAAA+AGG - chr7.2:18633172-18633191 MS.gene014011:CDS 35.0%
AATTGATATGAACCTTCCAG+TGG + chr7.2:18630696-18630715 None:intergenic 35.0%
ATTCTCATTCCTTTGTGTTC+AGG + chr7.2:18632952-18632971 None:intergenic 35.0%
ATTGAACAGATGCAAAACGA+GGG - chr7.2:18632514-18632533 MS.gene014011:intron 35.0%
ATTGATATGAACCTTCCAGT+GGG + chr7.2:18630695-18630714 None:intergenic 35.0%
CAAATAAACACACATACACG+GGG - chr7.2:18631065-18631084 MS.gene014011:CDS 35.0%
CAAGATGATTCCACACATAA+TGG + chr7.2:18633127-18633146 None:intergenic 35.0%
CATTATGTGTGGAATCATCT+TGG - chr7.2:18633125-18633144 MS.gene014011:intron 35.0%
GACTATTCCAAAGAAAAGGA+AGG - chr7.2:18631440-18631459 MS.gene014011:intron 35.0%
GAGCCCATCTATAAACAATT+GGG + chr7.2:18631748-18631767 None:intergenic 35.0%
GCATTGTAGGTAATGCTTAT+AGG - chr7.2:18633022-18633041 MS.gene014011:intron 35.0%
GCTTCCCAATTGTTTATAGA+TGG - chr7.2:18631741-18631760 MS.gene014011:intron 35.0%
GGAAGACTATTCCAAAGAAA+AGG - chr7.2:18631436-18631455 MS.gene014011:intron 35.0%
GTGTTTATTTGAGCATGTGT+TGG + chr7.2:18631056-18631075 None:intergenic 35.0%
TAAAATTTGCGGAGTGATGT+TGG - chr7.2:18632876-18632895 MS.gene014011:intron 35.0%
TATTGAACAGATGCAAAACG+AGG - chr7.2:18632513-18632532 MS.gene014011:intron 35.0%
TCTATACCTAATGTCTCTGA+TGG - chr7.2:18632356-18632375 MS.gene014011:CDS 35.0%
TCTTAACCATTCTTTGTGGA+GGG + chr7.2:18633077-18633096 None:intergenic 35.0%
TGAATTCCATCAGAGACATT+AGG + chr7.2:18632365-18632384 None:intergenic 35.0%
TGACTCTTAACCATTCTTTG+TGG + chr7.2:18633081-18633100 None:intergenic 35.0%
TGGCAAGCTTGTAAATTTCA+CGG + chr7.2:18631556-18631575 None:intergenic 35.0%
TTCTATTTGTAAAAGGCCAC+CGG - chr7.2:18632906-18632925 MS.gene014011:intron 35.0%
! ACTATTAGCTTCAGCATCTT+AGG - chr7.2:18631867-18631886 MS.gene014011:intron 35.0%
! AGGGTATAACTCAAAGGTTT+TGG - chr7.2:18631942-18631961 MS.gene014011:intron 35.0%
! ATGCTCAACAAATTAGCTTC+AGG - chr7.2:18632634-18632653 MS.gene014011:intron 35.0%
! CACCTTTTTCTTCCTTTAAC+CGG + chr7.2:18633177-18633196 None:intergenic 35.0%
! CACTAGAACATTTTCAGATG+TGG - chr7.2:18632657-18632676 MS.gene014011:intron 35.0%
! CCGATGGTTCTATTTGTAAA+AGG - chr7.2:18632899-18632918 MS.gene014011:intron 35.0%
! GATTCCACACATAATGGTTT+TGG + chr7.2:18633121-18633140 None:intergenic 35.0%
! GGACCCATTTAAAATGGAAT+TGG + chr7.2:18632177-18632196 None:intergenic 35.0%
! GTGTCGCATTTTACGAAATT+TGG - chr7.2:18632405-18632424 MS.gene014011:CDS 35.0%
! GTTAAGAGTCATGTTGATGA+GGG - chr7.2:18633090-18633109 MS.gene014011:intron 35.0%
! TTAATTTGAACACACGGGAT+AGG + chr7.2:18632795-18632814 None:intergenic 35.0%
!! CCTGTTTAATTTGAACACAC+GGG + chr7.2:18632800-18632819 None:intergenic 35.0%
AAACCACAACTATGCGATGA+GGG + chr7.2:18631583-18631602 None:intergenic 40.0%
AAGGTTGTGAATCCTGTATC+TGG + chr7.2:18631471-18631490 None:intergenic 40.0%
AATTAAACAGGTCACGTCCT+AGG - chr7.2:18632809-18632828 MS.gene014011:intron 40.0%
ACACAATCAGCAATGGTACA+CGG - chr7.2:18632590-18632609 MS.gene014011:intron 40.0%
ACCACTAGACTCTAATAGCA+AGG - chr7.2:18631151-18631170 MS.gene014011:CDS 40.0%
AGATGACTCTTCTCTACGAA+CGG - chr7.2:18631685-18631704 MS.gene014011:intron 40.0%
ATCCACTTGTAGAAGACAAC+CGG - chr7.2:18633155-18633174 MS.gene014011:intron 40.0%
ATCGCCAAAACCATTATGTG+TGG - chr7.2:18633114-18633133 MS.gene014011:intron 40.0%
ATCTGGTTACCTTCTTACCT+TGG - chr7.2:18631359-18631378 MS.gene014011:intron 40.0%
CACAACCTTCCTCAGAAATT+GGG - chr7.2:18631482-18631501 MS.gene014011:intron 40.0%
CCCGTGTGTTCAAATTAAAC+AGG - chr7.2:18632797-18632816 MS.gene014011:intron 40.0%
CTCTTAACCATTCTTTGTGG+AGG + chr7.2:18633078-18633097 None:intergenic 40.0%
GAATAGTCTTCCCAATCAGA+AGG + chr7.2:18631428-18631447 None:intergenic 40.0%
GCTATTAGAGTCTAGTGGTT+TGG + chr7.2:18631150-18631169 None:intergenic 40.0%
GGAGCCCATCTATAAACAAT+TGG + chr7.2:18631749-18631768 None:intergenic 40.0%
GGTGAAAGGTAGAAGCAATT+TGG + chr7.2:18632683-18632702 None:intergenic 40.0%
GGTTAAGAGTCATGTTGATG+AGG - chr7.2:18633089-18633108 MS.gene014011:intron 40.0%
GTTACCTTCTTACCTTGGTT+TGG - chr7.2:18631364-18631383 MS.gene014011:intron 40.0%
TAAACCACAACTATGCGATG+AGG + chr7.2:18631584-18631603 None:intergenic 40.0%
TCACAACCTTCCTCAGAAAT+TGG - chr7.2:18631481-18631500 MS.gene014011:intron 40.0%
TCTTATCGGTCCTTCTGATT+GGG - chr7.2:18631415-18631434 MS.gene014011:intron 40.0%
TGTAGAAGACAACCGGTTAA+AGG - chr7.2:18633162-18633181 MS.gene014011:intron 40.0%
TGTGGAATCATCTTGGATGA+TGG - chr7.2:18633132-18633151 MS.gene014011:intron 40.0%
TTATTTGGTTATTGGCCCAG+TGG - chr7.2:18631030-18631049 MS.gene014011:CDS 40.0%
TTCTTATCGGTCCTTCTGAT+TGG - chr7.2:18631414-18631433 MS.gene014011:intron 40.0%
! AAATGGGTCCAGTTTTGCTT+TGG - chr7.2:18632187-18632206 MS.gene014011:CDS 40.0%
! GGCAGCTTGCTTGATTTAAT+TGG + chr7.2:18632724-18632743 None:intergenic 40.0%
! GGTAGATCCTTCCTTTTCTT+TGG + chr7.2:18631450-18631469 None:intergenic 40.0%
! TCACATTGTCAGCTTTTCCA+AGG + chr7.2:18631607-18631626 None:intergenic 40.0%
!! AAAAGGTGCCAGGAACATAA+AGG - chr7.2:18633189-18633208 MS.gene014011:CDS 40.0%
!! AAAGGTGCCAGGAACATAAA+GGG - chr7.2:18633190-18633209 MS.gene014011:CDS 40.0%
!! TAAAGGAAGAAAAAGGTGCC+AGG - chr7.2:18633179-18633198 MS.gene014011:CDS 40.0%
!! TATTAGGCTTTAGGCATCAC+TGG + chr7.2:18630822-18630841 None:intergenic 40.0%
!! TCCTTGCTATTAGAGTCTAG+TGG + chr7.2:18631155-18631174 None:intergenic 40.0%
!!! CACCTCACTCTTTTTTCAAC+CGG + chr7.2:18632928-18632947 None:intergenic 40.0%
AAACTGGATGAAGCTGACAG+TGG - chr7.2:18632757-18632776 MS.gene014011:intron 45.0%
AACCCAATACACAGCACTTC+AGG + chr7.2:18632213-18632232 None:intergenic 45.0%
AACCGGTTGTCTTCTACAAG+TGG + chr7.2:18633160-18633179 None:intergenic 45.0%
AACGGGAAGGTCATTGTTTC+AGG - chr7.2:18631703-18631722 MS.gene014011:intron 45.0%
ACTTCAGGCCAAAGCAAAAC+TGG + chr7.2:18632198-18632217 None:intergenic 45.0%
AGAAGACCCTCCACAAAGAA+TGG - chr7.2:18633068-18633087 MS.gene014011:intron 45.0%
AGGAAGGATCTACCAGATAC+AGG - chr7.2:18631456-18631475 MS.gene014011:intron 45.0%
AGGATAGTTCAGGATACCAC+TGG - chr7.2:18632829-18632848 MS.gene014011:intron 45.0%
ATAAACACACATACACGGGG+AGG - chr7.2:18631068-18631087 MS.gene014011:CDS 45.0%
ATAGTTCAGGATACCACTGG+TGG - chr7.2:18632832-18632851 MS.gene014011:intron 45.0%
CAGCTGAGATTCTGTTCTCA+GGG + chr7.2:18632545-18632564 None:intergenic 45.0%
CGCATAGTTGTGGTTTACCT+TGG - chr7.2:18631587-18631606 MS.gene014011:intron 45.0%
CTTGGGTGATCTGATTGCTT+TGG + chr7.2:18632989-18633008 None:intergenic 45.0%
GATGACTCTTCTCTACGAAC+GGG - chr7.2:18631686-18631705 MS.gene014011:intron 45.0%
GCAACCAAACCAAGGTAAGA+AGG + chr7.2:18631371-18631390 None:intergenic 45.0%
GCATGGAACACAATCAGCAA+TGG - chr7.2:18632583-18632602 MS.gene014011:intron 45.0%
GTGGTATCCTGAACTATCCT+AGG + chr7.2:18632829-18632848 None:intergenic 45.0%
TAAACACACATACACGGGGA+GGG - chr7.2:18631069-18631088 MS.gene014011:CDS 45.0%
TAACACGAGCAACCAAACCA+AGG + chr7.2:18631379-18631398 None:intergenic 45.0%
TCCAGTTTGTCATCTGTCAG+AGG + chr7.2:18632745-18632764 None:intergenic 45.0%
TGGTCGTCAACAGATTGAAG+CGG - chr7.2:18630632-18630651 MS.gene014011:CDS 45.0%
TTCTCCTCCCTTTATGTTCC+TGG + chr7.2:18633200-18633219 None:intergenic 45.0%
! CACCGGTTGAAAAAAGAGTG+AGG - chr7.2:18632923-18632942 MS.gene014011:intron 45.0%
! CTACATCATGGCTGGCATTT+TGG + chr7.2:18633050-18633069 None:intergenic 45.0%
! GACATTTTACGTGGAATCCC+AGG + chr7.2:18631120-18631139 None:intergenic 45.0%
! TGATGGCGTAGTGCTTAAAG+AGG - chr7.2:18630959-18630978 MS.gene014011:CDS 45.0%
!! AGTCTAGTGGTTTGGCTTTG+AGG + chr7.2:18631142-18631161 None:intergenic 45.0%
!! ATGATTCTAGCTTCTCCCAC+TGG - chr7.2:18630677-18630696 MS.gene014011:CDS 45.0%
!!! CTCACTCTTTTTTCAACCGG+TGG + chr7.2:18632925-18632944 None:intergenic 45.0%
!!! GGCTTTGAGGACATTTTACG+TGG + chr7.2:18631129-18631148 None:intergenic 45.0%
AAACACACATACACGGGGAG+GGG - chr7.2:18631070-18631089 MS.gene014011:CDS 50.0%
AACTATGCGATGAGGGTCAG+TGG + chr7.2:18631576-18631595 None:intergenic 50.0%
ACCTTTCACCCAGAGGAAAG+TGG - chr7.2:18632693-18632712 MS.gene014011:intron 50.0%
ACTAGTGGCAAGCAAGAGAC+GGG + chr7.2:18632312-18632331 None:intergenic 50.0%
ACTCTTCTCTACGAACGGGA+AGG - chr7.2:18631690-18631709 MS.gene014011:intron 50.0%
AGAGCAAGACTCCAGCGTTA+TGG - chr7.2:18631626-18631645 MS.gene014011:intron 50.0%
AGCTGTGTCGCGTAGTAATC+TGG - chr7.2:18631529-18631548 MS.gene014011:intron 50.0%
AGGGTCTTCTACATCATGGC+TGG + chr7.2:18633058-18633077 None:intergenic 50.0%
CAATCAGCAATGGTACACGG+AGG - chr7.2:18632593-18632612 MS.gene014011:intron 50.0%
CCTTCCTCAGAAATTGGGTC+CGG - chr7.2:18631487-18631506 MS.gene014011:intron 50.0%
CCTTTCACCCAGAGGAAAGT+GGG - chr7.2:18632694-18632713 MS.gene014011:intron 50.0%
CTTCCTCAGAAATTGGGTCC+GGG - chr7.2:18631488-18631507 MS.gene014011:intron 50.0%
CTTTCACCCAGAGGAAAGTG+GGG - chr7.2:18632695-18632714 MS.gene014011:intron 50.0%
GACTAGTGGCAAGCAAGAGA+CGG + chr7.2:18632313-18632332 None:intergenic 50.0%
GCAGCTGAGATTCTGTTCTC+AGG + chr7.2:18632546-18632565 None:intergenic 50.0%
GCCTCTGACAGATGACAAAC+TGG - chr7.2:18632741-18632760 MS.gene014011:intron 50.0%
GCTGTGTCGCGTAGTAATCT+GGG - chr7.2:18631530-18631549 MS.gene014011:intron 50.0%
GGGTGATCTGATTGCTTTGG+CGG + chr7.2:18632986-18633005 None:intergenic 50.0%
GGTCGTCAACAGATTGAAGC+GGG - chr7.2:18630633-18630652 MS.gene014011:CDS 50.0%
GTCACGTCCTAGGATAGTTC+AGG - chr7.2:18632819-18632838 MS.gene014011:intron 50.0%
GTGGAGGGTCTTCTACATCA+TGG + chr7.2:18633062-18633081 None:intergenic 50.0%
TGACCCTCATCGCATAGTTG+TGG - chr7.2:18631577-18631596 MS.gene014011:intron 50.0%
TGATCTGATTGCTTTGGCGG+CGG + chr7.2:18632983-18633002 None:intergenic 50.0%
TGCTTCTACCTTTCACCCAG+AGG - chr7.2:18632686-18632705 MS.gene014011:intron 50.0%
TGGAATCCCAGGTTTGATCC+CGG + chr7.2:18631109-18631128 None:intergenic 50.0%
TGGTATCCCCACTTTCCTCT+GGG + chr7.2:18632704-18632723 None:intergenic 50.0%
TTCCTCAGAAATTGGGTCCG+GGG - chr7.2:18631489-18631508 MS.gene014011:intron 50.0%
TTGGTATCCCCACTTTCCTC+TGG + chr7.2:18632705-18632724 None:intergenic 50.0%
! GTGAGGTGTCCTGAACACAA+AGG - chr7.2:18632940-18632959 MS.gene014011:intron 50.0%
! TTGAGCATGTGTTGGCCACT+GGG + chr7.2:18631048-18631067 None:intergenic 50.0%
! TTTGAGCATGTGTTGGCCAC+TGG + chr7.2:18631049-18631068 None:intergenic 50.0%
!! GGCCTGAAGTGCTGTGTATT+GGG - chr7.2:18632208-18632227 MS.gene014011:CDS 50.0%
!! TGGCCTGAAGTGCTGTGTAT+TGG - chr7.2:18632207-18632226 MS.gene014011:CDS 50.0%
!! TTCTAGCTTCTCCCACTGGA+AGG - chr7.2:18630681-18630700 MS.gene014011:CDS 50.0%
AACACACATACACGGGGAGG+GGG - chr7.2:18631071-18631090 MS.gene014011:CDS 55.0%
ATGAGTGGCACTACCACCAG+TGG + chr7.2:18632848-18632867 None:intergenic 55.0%
CAAGACTCCAGCGTTATGGC+AGG - chr7.2:18631630-18631649 MS.gene014011:intron 55.0%
CCCACTTTCCTCTGGGTGAA+AGG + chr7.2:18632697-18632716 None:intergenic 55.0%
CTCCAGCGTTATGGCAGGAA+TGG - chr7.2:18631635-18631654 MS.gene014011:intron 55.0%
CTCCATTCCTGCCATAACGC+TGG + chr7.2:18631640-18631659 None:intergenic 55.0%
GGAATCCCAGGTTTGATCCC+GGG + chr7.2:18631108-18631127 None:intergenic 55.0%
GGTGCCAGGAACATAAAGGG+AGG - chr7.2:18633193-18633212 MS.gene014011:CDS 55.0%
GTTGACCCGGGATCAAACCT+GGG - chr7.2:18631100-18631119 MS.gene014011:CDS 55.0%
TCCTCAGAAATTGGGTCCGG+GGG - chr7.2:18631490-18631509 MS.gene014011:intron 55.0%
TGTTGACCCGGGATCAAACC+TGG - chr7.2:18631099-18631118 MS.gene014011:CDS 55.0%
! CCGGACCCAATTTCTGAGGA+AGG + chr7.2:18631490-18631509 None:intergenic 55.0%
ACCCCCGGACCCAATTTCTG+AGG + chr7.2:18631494-18631513 None:intergenic 60.0%
ACTCCAAGCTCGTACACCCC+CGG + chr7.2:18631509-18631528 None:intergenic 60.0%
TGCGGAGTGATGTTGGCCGA+TGG - chr7.2:18632883-18632902 MS.gene014011:intron 60.0%
! AGGGGGAAGTGTGTTGACCC+GGG - chr7.2:18631088-18631107 MS.gene014011:CDS 60.0%
! GAGGGGGAAGTGTGTTGACC+CGG - chr7.2:18631087-18631106 MS.gene014011:CDS 60.0%
! GGTCCGGGGGTGTACGAGCT+TGG - chr7.2:18631503-18631522 MS.gene014011:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 18630631 18633242 18630631 ID=MS.gene014011
chr7.2 mRNA 18630631 18633242 18630631 ID=MS.gene014011.t1;Parent=MS.gene014011
chr7.2 exon 18633171 18633242 18633171 ID=MS.gene014011.t1.exon1;Parent=MS.gene014011.t1
chr7.2 CDS 18633171 18633242 18633171 ID=cds.MS.gene014011.t1;Parent=MS.gene014011.t1
chr7.2 exon 18632104 18632460 18632104 ID=MS.gene014011.t1.exon2;Parent=MS.gene014011.t1
chr7.2 CDS 18632104 18632460 18632104 ID=cds.MS.gene014011.t1;Parent=MS.gene014011.t1
chr7.2 exon 18630631 18631350 18630631 ID=MS.gene014011.t1.exon3;Parent=MS.gene014011.t1
chr7.2 CDS 18630631 18631350 18630631 ID=cds.MS.gene014011.t1;Parent=MS.gene014011.t1
Gene Sequence

>MS.gene014011

ATGGTCGTCAACAGATTGAAGCGGGAGCAATGTGACCGAACAAAACATGATTCTAGCTTCTCCCACTGGAAGGTTCATATCAATTTTCTATATATCTTTTCTATTGTTAATAATAATGTGTTGTGTTTCTTATGTTAATGTTTCTACGAATCTGAGGTAAATAGAGTCTTGTATTTATAAAATATGTGCCAGTGATGCCTAAAGCCTAATACTTCATTCTTTTAGTGATTATTGTTAGTGTTAATTCTCCTTCCATTTAGATAATTTTCTGGGCTGCCACTTTTTAAAGTTTTCAATTTATACTTTGAAGAAAAAGCTATTGATTGTATGATGGCGTAGTGCTTAAAGAGGATGTGTGATGTGTGTCAGACTAAAAAATCTCAAGAACAAGATATTTTTTTATTTGGTTATTGGCCCAGTGGCCAACACATGCTCAAATAAACACACATACACGGGGAGGGGGAAGTGTGTTGACCCGGGATCAAACCTGGGATTCCACGTAAAATGTCCTCAAAGCCAAACCACTAGACTCTAATAGCAAGGACGAAAAGAATTCATCTCTAAGTCATTGAATTGATGACAAAAAATTAATTGAATGAATAAGTTTTACATGAAACTGAGTTTATATACTCCATATTATAGTGAAGTTAACAACTAAGCTAAGTTGTTATTACTAAATAACTAAAGCCATAATAGAATGACGAAAACTAGTTCTTTCAACTGTACAAATCTGGTTACCTTCTTACCTTGGTTTGGTTGCTCGTGTTATAAACTGTTTTCAGATTCTTATCGGTCCTTCTGATTGGGAAGACTATTCCAAAGAAAAGGAAGGATCTACCAGATACAGGATTCACAACCTTCCTCAGAAATTGGGTCCGGGGGTGTACGAGCTTGGAGTAGCTGTGTCGCGTAGTAATCTGGGCCGTGAAATTTACAAGCTTGCCACTGACCCTCATCGCATAGTTGTGGTTTACCTTGGAAAAGCTGACAATGTGAGAGCAAGACTCCAGCGTTATGGCAGGAATGGAGCTCATTTGAATAACACTTGTATGTCAGATGACTCTTCTCTACGAACGGGAAGGTCATTGTTTCAGGAGATATTTTCACAAGGCTTCCCAATTGTTTATAGATGGGCTCCTGTAAGTACATATCTGATATTTCATTTGCATTTCAGATTTAATATCAAGATGATTGCAACTGAACATGTATTAATGGGTTCGGAAGTTAAGCTAGATGACTATTAGCTTCAGCATCTTAGGTTCGTTTCAAAAAAGTCATTGATTGGAAATGCACGTTATCATATGATAGTATAGGGTATAACTCAAAGGTTTTGGATAAATCAGATAAAATTTGACCTCCATTCTTAAGCTATGAATTAGCAGTGCTCATTTAAAGGCCTGAAATCTATGTAATTTAGGTTGTAATTTTAAGAGTTATGTTTGAATTTGAGTATATAGTGAAATACTAAACATATTTCATTTAATTCTTAGTCATAATTGAATGATCATTTCATTATTGTATCTAGATTGTTCTTAGAGCTAACCCAATTCCATTTTAAATGGGTCCAGTTTTGCTTTGGCCTGAAGTGCTGTGTATTGGGTTTGTTCAGTTCTAGAGATATGTTAGGATCCAAAAGTGACACAATCAAAATTTAGGACAAGGTTAGGGATAGCTATAACCCGTCTCTTGCTTGCCACTAGTCTATTATGATAATTCATATTTATGTCTATACCTAATGTCTCTGATGGAATTCAAAATCTACTGTTAATATGTGGTGTCGCATTTTACGAAATTTGGCACAAAATACATTTGCATCTTACACAAAAGATATATAAAAGTGCAATTTTTTGGAACTATAATGGTTTATCTGTGCAATGTTGTTTATTGAACAGATGCAAAACGAGGGAGATGCCCTGAGAACAGAATCTCAGCTGCTTAGTACATTTGATTATGCATGGAACACAATCAGCAATGGTACACGGAGGCCTGATGATATTCTTCAAATGCTCAACAAATTAGCTTCAGGCACTAGAACATTTTCAGATGTGGCCAAATTGCTTCTACCTTTCACCCAGAGGAAAGTGGGGATACCAATTAAATCAAGCAAGCTGCCTCTGACAGATGACAAACTGGATGAAGCTGACAGTGGCAGCTACAACGTCCTATCCCGTGTGTTCAAATTAAACAGGTCACGTCCTAGGATAGTTCAGGATACCACTGGTGGTAGTGCCACTCATGAGAATGCTAAAATTTGCGGAGTGATGTTGGCCGATGGTTCTATTTGTAAAAGGCCACCGGTTGAAAAAAGAGTGAGGTGTCCTGAACACAAAGGAATGAGAATAAATGCGTCCGCCGCCAAAGCAATCAGATCACCCAAGTCAGAATTAGAAAGCATTGTAGGTAATGCTTATAGGTACCAAAATGCCAGCCATGATGTAGAAGACCCTCCACAAAGAATGGTTAAGAGTCATGTTGATGAGGGTATCGCCAAAACCATTATGTGTGGAATCATCTTGGATGATGGATCCACTTGTAGAAGACAACCGGTTAAAGGAAGAAAAAGGTGCCAGGAACATAAAGGGAGGAGAATTCGTGCAAATTCGAAAAAATAA
Protein sequence

>MS.gene014011.t1

MVVNRLKREQCDRTKHDSSFSHWKILIGPSDWEDYSKEKEGSTRYRIHNLPQKLGPGVYELGVAVSRSNLGREIYKLATDPHRIVVVYLGKADNVRARLQRYGRNGAHLNNTCMSDDSSLRTGRSLFQEIFSQGFPIVYRWAPMQNEGDALRTESQLLSTFDYAWNTISNGTRRPDDILQMLNKLASGTRTFSDVAKLLLPFTQRKVGIPIKSSKLPLTDDKLDEADSGSYNVLSRVFKLNRSRPRIVQDTTGGSATHENAKICGVMLADGSICKRPPVEKRVRCPEHKGMRINASAAKAIRSPKSELESIVGNAYRYQNASHDVEDPPQRMVKSHVDEGIAKTIMCGIILDDGSTCRRQPVKGRKRCQEHKGRRIRANSKK