Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014248.t1 | AES92029.1 | 80.8 | 213 | 30 | 1 | 1 | 213 | 24 | 225 | 1.60E-97 | 365.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014248.t1 | G7JQ00 | 80.8 | 213 | 30 | 1 | 1 | 213 | 24 | 225 | 1.2e-97 | 365.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014248.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014248.t1 | MTR_4g123470 | 80.751 | 213 | 30 | 1 | 1 | 213 | 24 | 225 | 3.26e-126 | 355 |
| MS.gene014248.t1 | MTR_1g090753 | 38.462 | 104 | 64 | 0 | 6 | 109 | 43 | 146 | 5.06e-20 | 83.6 |
| MS.gene014248.t1 | MTR_7g025150 | 28.205 | 195 | 129 | 2 | 5 | 199 | 25 | 208 | 6.15e-19 | 82.0 |
| MS.gene014248.t1 | MTR_4g085790 | 27.692 | 195 | 130 | 2 | 5 | 199 | 25 | 208 | 7.10e-18 | 79.0 |
| MS.gene014248.t1 | MTR_7g113060 | 83.784 | 37 | 6 | 0 | 175 | 211 | 3 | 39 | 1.11e-14 | 69.7 |
| MS.gene014248.t1 | MTR_7g039210 | 30.631 | 111 | 77 | 0 | 1 | 111 | 22 | 132 | 5.75e-14 | 68.9 |
| MS.gene014248.t1 | MTR_8g468550 | 41.772 | 79 | 46 | 0 | 9 | 87 | 65 | 143 | 6.81e-14 | 67.0 |
| MS.gene014248.t1 | MTR_1g054485 | 37.079 | 89 | 56 | 0 | 8 | 96 | 28 | 116 | 5.77e-13 | 65.1 |
| MS.gene014248.t1 | MTR_0014s0220 | 32.673 | 101 | 66 | 1 | 7 | 105 | 645 | 745 | 1.85e-12 | 66.2 |
| MS.gene014248.t1 | MTR_4g053550 | 42.029 | 69 | 40 | 0 | 5 | 73 | 48 | 116 | 1.92e-12 | 62.4 |
| MS.gene014248.t1 | MTR_5g074790 | 31.313 | 99 | 68 | 0 | 5 | 103 | 172 | 270 | 2.09e-12 | 64.7 |
| MS.gene014248.t1 | MTR_1g054495 | 27.919 | 197 | 120 | 6 | 8 | 200 | 28 | 206 | 5.14e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGTTCCTATCTGGATA+TGG | 0.289906 | 3.3:+95251165 | MS.gene014248:CDS |
| GTACCTTTGAAGTTCCTATC+TGG | 0.333773 | 3.3:+95251159 | MS.gene014248:CDS |
| AGGAATTTGCCGAAAGTATC+AGG | 0.335653 | 3.3:-95251311 | None:intergenic |
| AATCGTGATGTTTCACTCTA+AGG | 0.341127 | 3.3:-95251331 | None:intergenic |
| TAGGAACTACCTGATACTTT+CGG | 0.400617 | 3.3:+95251302 | MS.gene014248:intron |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 3.3:-95251417 | None:intergenic |
| ATCTACTTCCACCAAGGTAC+TGG | 0.428620 | 3.3:-95251052 | None:intergenic |
| TTTCTATTTAAATATGATGA+AGG | 0.442085 | 3.3:+95250709 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 3.3:-95251416 | None:intergenic |
| GAGCAATGGCACTTGCTAGA+TGG | 0.455163 | 3.3:+95250912 | MS.gene014248:CDS |
| TTGTGCTGCCAATGGTATTG+AGG | 0.463000 | 3.3:+95251457 | MS.gene014248:CDS |
| AAATATTTAGCAACAAAGAA+TGG | 0.465634 | 3.3:-95250876 | None:intergenic |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 3.3:+95251422 | MS.gene014248:CDS |
| TGCCGCACTCATGGAATGAT+AGG | 0.476633 | 3.3:-95251123 | None:intergenic |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 3.3:+95251416 | MS.gene014248:CDS |
| TCGTCGATCCATATCCAGAT+AGG | 0.486078 | 3.3:-95251173 | None:intergenic |
| GGCCGTTGTTGACAGATGGT+TGG | 0.487397 | 3.3:+95250979 | MS.gene014248:CDS |
| ACACGATGTTGCCGCACTCA+TGG | 0.490628 | 3.3:-95251132 | None:intergenic |
| GTCATGCGTTTATCAACGCT+TGG | 0.500774 | 3.3:-95251540 | None:intergenic |
| TGTGCTGCCAATGGTATTGA+GGG | 0.501205 | 3.3:+95251458 | MS.gene014248:CDS |
| CACGATTACGTCATGTTGTA+CGG | 0.501494 | 3.3:+95251347 | MS.gene014248:CDS |
| TAAATATTTCTACGAGCTAC+CGG | 0.507354 | 3.3:+95250890 | MS.gene014248:CDS |
| ATTATTGCTTTCCAGTACCT+TGG | 0.508515 | 3.3:+95251041 | MS.gene014248:CDS |
| TGATAGGGTGGATAATCAGT+TGG | 0.521361 | 3.3:-95251107 | None:intergenic |
| ACAGGAATGATATTGTCATA+AGG | 0.528831 | 3.3:-95251371 | None:intergenic |
| AGTCGGCCGTTGTTGACAGA+TGG | 0.531450 | 3.3:+95250975 | MS.gene014248:CDS |
| TCTAGCAAGTGCCATTGCTC+CGG | 0.534587 | 3.3:-95250909 | None:intergenic |
| TTCTATTTAAATATGATGAA+GGG | 0.535303 | 3.3:+95250710 | None:intergenic |
| ATTGCTTTCCAGTACCTTGG+TGG | 0.549152 | 3.3:+95251044 | MS.gene014248:CDS |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 3.3:-95251415 | None:intergenic |
| GTCTCCGCCCTCAATACCAT+TGG | 0.581950 | 3.3:-95251465 | None:intergenic |
| CTGACTAACTTCTAGGATGT+CGG | 0.584798 | 3.3:+95250843 | MS.gene014248:intron |
| TGGCACTTGCTAGATGGTGA+TGG | 0.587936 | 3.3:+95250918 | MS.gene014248:CDS |
| TATCCAGATAGGAACTTCAA+AGG | 0.596572 | 3.3:-95251162 | None:intergenic |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 3.3:+95251426 | MS.gene014248:CDS |
| GGCACTTGCTAGATGGTGAT+GGG | 0.608333 | 3.3:+95250919 | MS.gene014248:CDS |
| TGTTCAACAAATCAGTTAGT+CGG | 0.609582 | 3.3:+95250958 | MS.gene014248:CDS |
| TCTACGAGCTACCGGAGCAA+TGG | 0.612965 | 3.3:+95250898 | MS.gene014248:CDS |
| ACCCTATCATTCCATGAGTG+CGG | 0.636716 | 3.3:+95251121 | MS.gene014248:CDS |
| GCCGCACTCATGGAATGATA+GGG | 0.643165 | 3.3:-95251122 | None:intergenic |
| TTTCAAGTACAAGTGTGTCG+TGG | 0.648578 | 3.3:+95251496 | MS.gene014248:CDS |
| GCACTCATGGAATGATAGGG+TGG | 0.649652 | 3.3:-95251119 | None:intergenic |
| TTTGAAATCTACTTCCACCA+AGG | 0.681040 | 3.3:-95251058 | None:intergenic |
| CTCCAACCATCTGTCAACAA+CGG | 0.696054 | 3.3:-95250981 | None:intergenic |
| TGGATCGACGAAATCACCGA+AGG | 0.700849 | 3.3:+95251185 | MS.gene014248:CDS |
| GCTGCCAATGGTATTGAGGG+CGG | 0.739556 | 3.3:+95251461 | MS.gene014248:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTATATATGCTATAATAA+AGG | + | chr3.3:95251242-95251261 | MS.gene014248:intron | 15.0% |
| !!! | CAGTTTTTTTTCTTTTTTTT+AGG | + | chr3.3:95251283-95251302 | MS.gene014248:intron | 15.0% |
| !! | AAAAAACAATAAACAACCTT+CGG | - | chr3.3:95251204-95251223 | None:intergenic | 20.0% |
| !! | AAATATTTAGCAACAAAGAA+TGG | - | chr3.3:95250879-95250898 | None:intergenic | 20.0% |
| !!! | ATGAAAATTAAATCCTCTTT+TGG | + | chr3.3:95250755-95250774 | MS.gene014248:intron | 20.0% |
| !!! | TTTTTATGCTATTCATTTAG+AGG | + | chr3.3:95251220-95251239 | MS.gene014248:CDS | 20.0% |
| ! | ATATTAAATCCAACCAAAAG+AGG | - | chr3.3:95250771-95250790 | None:intergenic | 25.0% |
| !! | AAATTAAATCCTCTTTTGGT+TGG | + | chr3.3:95250759-95250778 | MS.gene014248:intron | 25.0% |
| !!! | TCTGTATATTTTAATCTCAC+AGG | - | chr3.3:95251392-95251411 | None:intergenic | 25.0% |
| ACAGGAATGATATTGTCATA+AGG | - | chr3.3:95251374-95251393 | None:intergenic | 30.0% | |
| TCATGCACATAATACGTTTA+CGG | - | chr3.3:95250739-95250758 | None:intergenic | 30.0% | |
| TGTTCAACAAATCAGTTAGT+CGG | + | chr3.3:95250958-95250977 | MS.gene014248:CDS | 30.0% | |
| ! | TAAATATTTCTACGAGCTAC+CGG | + | chr3.3:95250890-95250909 | MS.gene014248:CDS | 30.0% |
| ! | TAATACACTGACTAACTTCT+AGG | + | chr3.3:95250836-95250855 | MS.gene014248:intron | 30.0% |
| AATCGTGATGTTTCACTCTA+AGG | - | chr3.3:95251334-95251353 | None:intergenic | 35.0% | |
| ATTATTGCTTTCCAGTACCT+TGG | + | chr3.3:95251041-95251060 | MS.gene014248:CDS | 35.0% | |
| TAGGAACTACCTGATACTTT+CGG | + | chr3.3:95251302-95251321 | MS.gene014248:intron | 35.0% | |
| TATCCAGATAGGAACTTCAA+AGG | - | chr3.3:95251165-95251184 | None:intergenic | 35.0% | |
| TTGAAGTTCCTATCTGGATA+TGG | + | chr3.3:95251165-95251184 | MS.gene014248:CDS | 35.0% | |
| ! | TTTGAAATCTACTTCCACCA+AGG | - | chr3.3:95251061-95251080 | None:intergenic | 35.0% |
| ATGTGATGTTCGGAAAAGGT+TGG | + | chr3.3:95251426-95251445 | MS.gene014248:CDS | 40.0% | |
| CACGATTACGTCATGTTGTA+CGG | + | chr3.3:95251347-95251366 | MS.gene014248:CDS | 40.0% | |
| CCAAATGTGATGTTCGGAAA+AGG | + | chr3.3:95251422-95251441 | MS.gene014248:CDS | 40.0% | |
| CCCATACCAAATGTGATGTT+CGG | + | chr3.3:95251416-95251435 | MS.gene014248:CDS | 40.0% | |
| CCGAACATCACATTTGGTAT+GGG | - | chr3.3:95251419-95251438 | None:intergenic | 40.0% | |
| CGAACATCACATTTGGTATG+GGG | - | chr3.3:95251418-95251437 | None:intergenic | 40.0% | |
| TCCGAACATCACATTTGGTA+TGG | - | chr3.3:95251420-95251439 | None:intergenic | 40.0% | |
| TGATAGGGTGGATAATCAGT+TGG | - | chr3.3:95251110-95251129 | None:intergenic | 40.0% | |
| ! | CCTTTTCCGAACATCACATT+TGG | - | chr3.3:95251425-95251444 | None:intergenic | 40.0% |
| ! | TTTCAAGTACAAGTGTGTCG+TGG | + | chr3.3:95251496-95251515 | MS.gene014248:CDS | 40.0% |
| !! | AGGAATTTGCCGAAAGTATC+AGG | - | chr3.3:95251314-95251333 | None:intergenic | 40.0% |
| !! | CTGACTAACTTCTAGGATGT+CGG | + | chr3.3:95250843-95250862 | MS.gene014248:intron | 40.0% |
| !! | GTACCTTTGAAGTTCCTATC+TGG | + | chr3.3:95251159-95251178 | MS.gene014248:CDS | 40.0% |
| ACCCTATCATTCCATGAGTG+CGG | + | chr3.3:95251121-95251140 | MS.gene014248:CDS | 45.0% | |
| ATCTACTTCCACCAAGGTAC+TGG | - | chr3.3:95251055-95251074 | None:intergenic | 45.0% | |
| ATTGCTTTCCAGTACCTTGG+TGG | + | chr3.3:95251044-95251063 | MS.gene014248:CDS | 45.0% | |
| CTCCAACCATCTGTCAACAA+CGG | - | chr3.3:95250984-95251003 | None:intergenic | 45.0% | |
| TCGTCGATCCATATCCAGAT+AGG | - | chr3.3:95251176-95251195 | None:intergenic | 45.0% | |
| TTGTGCTGCCAATGGTATTG+AGG | + | chr3.3:95251457-95251476 | MS.gene014248:CDS | 45.0% | |
| ! | ACGAGTTTTTGTGCTGCCAA+TGG | + | chr3.3:95251449-95251468 | MS.gene014248:CDS | 45.0% |
| ! | TGTGCTGCCAATGGTATTGA+GGG | + | chr3.3:95251458-95251477 | MS.gene014248:CDS | 45.0% |
| GCACTCATGGAATGATAGGG+TGG | - | chr3.3:95251122-95251141 | None:intergenic | 50.0% | |
| GCCGCACTCATGGAATGATA+GGG | - | chr3.3:95251125-95251144 | None:intergenic | 50.0% | |
| TGCCGCACTCATGGAATGAT+AGG | - | chr3.3:95251126-95251145 | None:intergenic | 50.0% | |
| TGGATCGACGAAATCACCGA+AGG | + | chr3.3:95251185-95251204 | MS.gene014248:CDS | 50.0% | |
| ! | GAGCAATGGCACTTGCTAGA+TGG | + | chr3.3:95250912-95250931 | MS.gene014248:CDS | 50.0% |
| ! | GGCACTTGCTAGATGGTGAT+GGG | + | chr3.3:95250919-95250938 | MS.gene014248:CDS | 50.0% |
| ! | TCTAGCAAGTGCCATTGCTC+CGG | - | chr3.3:95250912-95250931 | None:intergenic | 50.0% |
| ! | TGGCACTTGCTAGATGGTGA+TGG | + | chr3.3:95250918-95250937 | MS.gene014248:CDS | 50.0% |
| ACACGATGTTGCCGCACTCA+TGG | - | chr3.3:95251135-95251154 | None:intergenic | 55.0% | |
| AGTCGGCCGTTGTTGACAGA+TGG | + | chr3.3:95250975-95250994 | MS.gene014248:CDS | 55.0% | |
| GGCCGTTGTTGACAGATGGT+TGG | + | chr3.3:95250979-95250998 | MS.gene014248:CDS | 55.0% | |
| GTCTCCGCCCTCAATACCAT+TGG | - | chr3.3:95251468-95251487 | None:intergenic | 55.0% | |
| TCTACGAGCTACCGGAGCAA+TGG | + | chr3.3:95250898-95250917 | MS.gene014248:CDS | 55.0% | |
| ! | GCTGCCAATGGTATTGAGGG+CGG | + | chr3.3:95251461-95251480 | MS.gene014248:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95250722 | 95251550 | 95250722 | ID=MS.gene014248 |
| chr3.3 | mRNA | 95250722 | 95251550 | 95250722 | ID=MS.gene014248.t1;Parent=MS.gene014248 |
| chr3.3 | exon | 95250722 | 95250733 | 95250722 | ID=MS.gene014248.t1.exon1;Parent=MS.gene014248.t1 |
| chr3.3 | CDS | 95250722 | 95250733 | 95250722 | ID=cds.MS.gene014248.t1;Parent=MS.gene014248.t1 |
| chr3.3 | exon | 95250858 | 95251241 | 95250858 | ID=MS.gene014248.t1.exon2;Parent=MS.gene014248.t1 |
| chr3.3 | CDS | 95250858 | 95251241 | 95250858 | ID=cds.MS.gene014248.t1;Parent=MS.gene014248.t1 |
| chr3.3 | exon | 95251305 | 95251550 | 95251305 | ID=MS.gene014248.t1.exon3;Parent=MS.gene014248.t1 |
| chr3.3 | CDS | 95251305 | 95251550 | 95251305 | ID=cds.MS.gene014248.t1;Parent=MS.gene014248.t1 |
| Gene Sequence |
| Protein sequence |
