AlfalfaGEDB | MS.gene014257

M. sativa cultivar XinJiangDaYe / MS.gene014257

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014257.t1	XP_003603971.2	96.1	155	6	0	1	155	1	155	8.00E-70	273.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014257.t1	C0Z2L5	44.6	157	78	4	2	149	9	165	3.8e-20	99.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014257.t1	G7J773	96.1	155	6	0	1	155	1	155	5.8e-70	273.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene014257.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014257.t1	MTR_3g117120	96.129	155	6	0	1	155	1	155	8.21e-90	258
MS.gene014257.t1	MTR_4g070860	70.588	153	42	1	1	150	1	153	1.63e-62	189
MS.gene014257.t1	MTR_1g022495	60.248	161	53	4	1	152	1	159	1.31e-55	172
MS.gene014257.t1	MTR_8g086020	52.795	161	69	4	1	155	1	160	1.78e-46	149
MS.gene014257.t1	MTR_3g112220	63.291	79	29	0	27	105	1	79	3.21e-29	103
MS.gene014257.t1	MTR_1g087040	44.954	109	57	1	1	109	1	106	9.26e-25	93.6
MS.gene014257.t1	MTR_6g016375	44.118	102	47	2	26	124	86	180	2.99e-18	77.8
MS.gene014257.t1	MTR_8g015250	50.685	73	36	0	26	98	70	142	2.74e-17	74.7
MS.gene014257.t1	MTR_7g029400	44.444	99	51	2	1	98	57	152	3.26e-17	75.1
MS.gene014257.t1	MTR_7g017880	51.250	80	39	0	19	98	76	155	5.31e-17	74.3
MS.gene014257.t1	MTR_4g097440	39.091	110	60	1	7	109	45	154	1.03e-15	70.5
MS.gene014257.t1	MTR_5g015090	41.053	95	48	1	27	121	58	144	2.83e-14	66.6
MS.gene014257.t1	MTR_3g109310	33.945	109	68	1	6	110	98	206	8.77e-13	64.7
MS.gene014257.t1	MTR_7g109510	43.802	121	61	2	27	147	19	132	3.97e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014257.t1	AT1G75390	53.750	160	57	5	2	148	9	164	6.40e-48	153
MS.gene014257.t1	AT4G34590	56.391	133	49	2	26	149	23	155	3.66e-44	143
MS.gene014257.t1	AT2G18160	46.897	145	55	4	22	146	23	165	3.04e-36	123
MS.gene014257.t1	AT1G75390	60.825	97	34	2	2	94	9	105	8.83e-33	113
MS.gene014257.t1	AT3G62420	44.203	138	75	1	13	150	8	143	9.30e-31	108
MS.gene014257.t1	AT2G04038	48.837	86	41	1	16	98	57	142	3.82e-18	76.6
MS.gene014257.t1	AT5G15830	34.694	147	79	3	9	155	55	184	3.01e-17	75.1
MS.gene014257.t1	AT1G13600	45.361	97	48	2	16	107	70	166	1.00e-16	73.9
MS.gene014257.t1	AT3G30530	52.055	73	35	0	26	98	77	149	2.43e-16	72.4
MS.gene014257.t1	AT5G38800	47.500	80	42	0	19	98	61	140	1.48e-15	70.1
MS.gene014257.t1	AT5G49450	35.922	103	63	1	27	126	13	115	1.94e-12	61.2
MS.gene014257.t1	AT4G37730	45.000	80	37	1	26	98	193	272	4.43e-12	62.8
MS.gene014257.t1	AT2G22850	37.079	89	56	0	23	111	122	210	1.54e-11	60.5
MS.gene014257.t1	AT2G22850	37.079	89	56	0	23	111	122	210	1.54e-11	60.5
MS.gene014257.t1	AT3G49760	34.524	84	55	0	28	111	70	153	4.05e-11	58.2
MS.gene014257.t1	AT1G68880	38.554	83	51	0	12	94	29	111	4.08e-11	57.8

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCCTCTGAACCTGAATT+TGG	0.134136	3.3:-95168881	None:intergenic
CTTCAACAACCCTTTGAATA+TGG	0.147562	3.3:+95169228	MS.gene014257:CDS
CCTCACAACTCAACGATTCT+TGG	0.288929	3.3:+95169063	MS.gene014257:CDS
TGTTCAACACTTCCAAATTC+AGG	0.300497	3.3:+95168869	MS.gene014257:CDS
CTTGGATGATCTTGCTGTTC+AGG	0.305122	3.3:+95168994	MS.gene014257:CDS
TCTTCAAGTGGAACATCTTC+AGG	0.306987	3.3:+95168845	MS.gene014257:CDS
TTCAACAACCCTTTGAATAT+GGG	0.356244	3.3:+95169229	MS.gene014257:CDS
TCATTATTATTATTATCAAC+AGG	0.406404	3.3:-95169199	None:intergenic
TTATTTGAACCAACCAATCA+TGG	0.433242	3.3:+95169252	MS.gene014257:CDS
TTCAAATAACCCATATTCAA+AGG	0.450188	3.3:-95169238	None:intergenic
ATATTTGCAGAAGCCATGAT+TGG	0.461206	3.3:-95169265	None:intergenic
GAGATCTTCTTGCTGATTCG+CGG	0.468319	3.3:-95168952	None:intergenic
GGAAGATTTGATGCTTCTGA+TGG	0.470481	3.3:+95168898	MS.gene014257:CDS
TCAAATAACCCATATTCAAA+GGG	0.472163	3.3:-95169237	None:intergenic
CTGTTCAGGTGAGTCAACTA+AGG	0.511341	3.3:+95169008	MS.gene014257:CDS
TTGCAGAAGCCATGATTGGT+TGG	0.539753	3.3:-95169261	None:intergenic
AGCTTCATGAATGTTGCAAA+TGG	0.559489	3.3:+95169166	MS.gene014257:CDS
TCTCACATGGCTTCTTCAAG+TGG	0.568523	3.3:+95168833	None:intergenic
ACTTCCAAATTCAGGTTCAG+AGG	0.572504	3.3:+95168877	MS.gene014257:CDS
CAGCAAGAAGATCTCGAATG+AGG	0.587735	3.3:+95168960	MS.gene014257:CDS
CCAAGAATCGTTGAGTTGTG+AGG	0.599678	3.3:-95169063	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCATTATTATTATTATCAAC+AGG	-	chr3.3:95169202-95169221	None:intergenic	15.0%
!	TCAAATAACCCATATTCAAA+GGG	-	chr3.3:95169240-95169259	None:intergenic	25.0%
!	TTCAAATAACCCATATTCAA+AGG	-	chr3.3:95169241-95169260	None:intergenic	25.0%
	AATGAGGAAACAAAAACACT+TGG	+	chr3.3:95168976-95168995	MS.gene014257:CDS	30.0%
	TTATTTGAACCAACCAATCA+TGG	+	chr3.3:95169252-95169271	MS.gene014257:CDS	30.0%
	TTCAACAACCCTTTGAATAT+GGG	+	chr3.3:95169229-95169248	MS.gene014257:CDS	30.0%
	AGCTTCATGAATGTTGCAAA+TGG	+	chr3.3:95169166-95169185	MS.gene014257:CDS	35.0%
	ATATTTGCAGAAGCCATGAT+TGG	-	chr3.3:95169268-95169287	None:intergenic	35.0%
	CTTCAACAACCCTTTGAATA+TGG	+	chr3.3:95169228-95169247	MS.gene014257:CDS	35.0%
	TGTTCAACACTTCCAAATTC+AGG	+	chr3.3:95168869-95168888	MS.gene014257:CDS	35.0%
	GGAAGATTTGATGCTTCTGA+TGG	+	chr3.3:95168898-95168917	MS.gene014257:CDS	40.0%
	TCTTCAAGTGGAACATCTTC+AGG	+	chr3.3:95168845-95168864	MS.gene014257:CDS	40.0%
	TCTTCCTCTGAACCTGAATT+TGG	-	chr3.3:95168884-95168903	None:intergenic	40.0%
!	ACTTCCAAATTCAGGTTCAG+AGG	+	chr3.3:95168877-95168896	MS.gene014257:CDS	40.0%
	CAGCAAGAAGATCTCGAATG+AGG	+	chr3.3:95168960-95168979	MS.gene014257:CDS	45.0%
	CCTCACAACTCAACGATTCT+TGG	+	chr3.3:95169063-95169082	MS.gene014257:CDS	45.0%
	CTGTTCAGGTGAGTCAACTA+AGG	+	chr3.3:95169008-95169027	MS.gene014257:CDS	45.0%
	TTGCAGAAGCCATGATTGGT+TGG	-	chr3.3:95169264-95169283	None:intergenic	45.0%
!	CCAAGAATCGTTGAGTTGTG+AGG	-	chr3.3:95169066-95169085	None:intergenic	45.0%
!!	CTTGGATGATCTTGCTGTTC+AGG	+	chr3.3:95168994-95169013	MS.gene014257:CDS	45.0%
!!	GAGATCTTCTTGCTGATTCG+CGG	-	chr3.3:95168955-95168974	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	95168839	95169306	95168839	ID=MS.gene014257
chr3.3	mRNA	95168839	95169306	95168839	ID=MS.gene014257.t1;Parent=MS.gene014257
chr3.3	exon	95168839	95169306	95168839	ID=MS.gene014257.t1.exon1;Parent=MS.gene014257.t1
chr3.3	CDS	95168839	95169306	95168839	ID=cds.MS.gene014257.t1;Parent=MS.gene014257.t1

Gene Sequence

>MS.gene014257

ATGGCTTCTTCAAGTGGAACATCTTCAGGCTGTTCAACACTTCCAAATTCAGGTTCAGAGGAAGATTTGATGCTTCTGATGGATCAAAGAAAAAGAAAGAGAATGATATCAAACCGCGAATCAGCAAGAAGATCTCGAATGAGGAAACAAAAACACTTGGATGATCTTGCTGTTCAGGTGAGTCAACTAAGGAATGAGAATCAACAGATTCTAACTTCAGTGAACCTCACAACTCAACGATTCTTGGCTGTTGAATCTGAAAACTCTGTGCTTAGAGCTCAACTGAATGAACTCAACAGCAGATTTGAGTCTTTGAATGAGATCATCAGCTTCATGAATGTTGCAAATGGTGTTTTTGAACCTGTTGATAATAATAATAATGACAATTACTTCAACAACCCTTTGAATATGGGTTATTTGAACCAACCAATCATGGCTTCTGCAAATATGAACATGATACATTATTGA

Protein sequence

>MS.gene014257.t1

MASSSGTSSGCSTLPNSGSEEDLMLLMDQRKRKRMISNRESARRSRMRKQKHLDDLAVQVSQLRNENQQILTSVNLTTQRFLAVESENSVLRAQLNELNSRFESLNEIISFMNVANGVFEPVDNNNNDNYFNNPLNMGYLNQPIMASANMNMIHY