AlfalfaGEDB | MS.gene014273

M. sativa cultivar XinJiangDaYe / MS.gene014273

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014273.t1	XP_024633411.1	100	81	0	0	1	81	1	81	2.20E-39	171

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014273.t1	F4JVB8	64.2	81	27	1	1	81	1	79	1.3e-24	113.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014273.t1	G7J732	100.0	81	0	0	1	81	1	81	1.6e-39	171.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene014273.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014273.t1	MTR_3g116720	100.000	81	0	0	1	81	1	81	1.08e-56	169
MS.gene014273.t1	MTR_1g022290	73.134	67	18	0	14	80	5	71	2.43e-34	112
MS.gene014273.t1	MTR_3g111880	73.846	65	17	0	14	78	5	69	8.46e-32	106
MS.gene014273.t1	MTR_7g089210	77.049	61	14	0	19	79	15	75	2.80e-30	102
MS.gene014273.t1	MTR_3g013440	62.963	81	23	2	1	80	1	75	6.35e-29	99.4
MS.gene014273.t1	MTR_8g077360	64.615	65	22	1	16	80	5	68	2.35e-27	94.7
MS.gene014273.t1	MTR_8g077380	64.615	65	22	1	16	80	5	68	2.42e-27	94.7
MS.gene014273.t1	MTR_8g077390	64.615	65	22	1	16	80	5	68	2.42e-27	94.7
MS.gene014273.t1	MTR_5g020170	60.938	64	25	0	16	79	19	82	5.42e-26	92.0
MS.gene014273.t1	MTR_6g004250	60.000	65	26	0	14	78	8	72	1.07e-25	90.9
MS.gene014273.t1	MTR_5g020540	68.852	61	18	1	16	76	4	63	1.56e-25	89.7
MS.gene014273.t1	MTR_8g077420	63.492	63	22	1	14	76	3	64	2.64e-25	89.4
MS.gene014273.t1	MTR_3g111920	53.846	65	30	0	16	80	3	67	7.00e-22	81.3
MS.gene014273.t1	MTR_8g063870	50.725	69	34	0	12	80	4	72	1.27e-20	82.4
MS.gene014273.t1	MTR_3g462790	57.576	66	28	0	16	81	43	108	2.65e-20	82.4
MS.gene014273.t1	MTR_5g069710	53.846	65	30	0	16	80	29	93	9.36e-20	80.9
MS.gene014273.t1	MTR_1g033600	48.529	68	35	0	14	81	10	77	9.90e-20	77.4
MS.gene014273.t1	MTR_8g063600	50.769	65	32	0	16	80	10	74	1.62e-19	79.7
MS.gene014273.t1	MTR_7g067080	51.471	68	33	0	14	81	33	100	2.02e-18	77.0
MS.gene014273.t1	MTR_5g081860	53.968	63	29	0	16	78	27	89	3.71e-18	75.9
MS.gene014273.t1	MTR_1g086180	59.649	57	22	1	16	71	11	67	2.97e-16	67.4
MS.gene014273.t1	MTR_1g111830	47.619	63	33	0	16	78	24	86	6.97e-16	69.7
MS.gene014273.t1	MTR_7g110580	57.627	59	24	1	16	73	8	66	4.36e-15	63.9
MS.gene014273.t1	MTR_4g111975	41.176	68	37	1	14	78	6	73	1.24e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014273.t1	AT1G75250	67.500	80	22	1	1	80	1	76	2.29e-35	116
MS.gene014273.t1	AT1G75250	67.500	80	22	1	1	80	1	76	2.86e-35	115
MS.gene014273.t1	AT4G39250	69.697	66	20	0	16	81	14	79	8.28e-34	112
MS.gene014273.t1	AT2G21650	66.667	66	22	0	16	81	14	79	1.81e-30	103
MS.gene014273.t1	AT1G19510	71.429	63	18	0	19	81	15	77	9.91e-30	101
MS.gene014273.t1	AT2G18328	69.841	63	19	0	19	81	14	76	7.29e-29	98.6
MS.gene014273.t1	AT4G36570	77.273	44	10	0	16	59	12	55	1.87e-21	79.3
MS.gene014273.t1	AT5G05790	52.941	68	32	0	14	81	29	96	2.67e-18	76.6
MS.gene014273.t1	AT5G05790	52.941	68	32	0	14	81	29	96	2.67e-18	76.6
MS.gene014273.t1	AT2G38090	53.846	65	30	0	16	80	29	93	1.40e-17	74.7
MS.gene014273.t1	AT3G11280	50.794	63	31	0	16	78	33	95	7.22e-17	72.4
MS.gene014273.t1	AT3G11280	50.794	63	31	0	16	78	33	95	7.22e-17	72.4
MS.gene014273.t1	AT5G58900	47.059	68	36	0	14	81	32	99	7.94e-17	72.8
MS.gene014273.t1	AT5G08520	49.254	67	34	0	14	80	10	76	3.19e-16	71.2
MS.gene014273.t1	AT5G04760	41.791	67	38	1	12	78	2	67	1.57e-13	62.8
MS.gene014273.t1	AT1G49010	42.647	68	36	1	14	78	6	73	6.13e-13	62.4
MS.gene014273.t1	AT5G01200	43.284	67	37	1	13	78	27	93	1.51e-12	60.8
MS.gene014273.t1	AT5G23650	39.189	74	45	0	5	78	2	75	1.16e-11	58.9

Find 16 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCACGTGGATTTGTAATT+TGG	0.099924	3.3:+94948758	None:intergenic
ACAAGCCTTGTGATTCAATT+TGG	0.187124	3.3:-94948958	MS.gene014273:CDS
GTGGATTTGTAATTTGGAAT+AGG	0.364725	3.3:+94948764	None:intergenic
AGCACTTGCAAAATTTGATA+AGG	0.422428	3.3:-94948906	MS.gene014273:CDS
CACAAGGCTTGTGTTTGCTA+AGG	0.474591	3.3:+94948969	None:intergenic
GATCTCAAACACATTGAGTC+TGG	0.498892	3.3:-94948794	MS.gene014273:CDS
GAGACACTATGAGCTACTCT+TGG	0.504426	3.3:-94948819	MS.gene014273:CDS
CTAATTTGCTACACTTCACG+TGG	0.522033	3.3:+94948745	None:intergenic
ACAAGGCTTGTGTTTGCTAA+GGG	0.544579	3.3:+94948970	None:intergenic
ACACTATGAGCTACTCTTGG+AGG	0.567606	3.3:-94948816	MS.gene014273:CDS
GTGTTTGCTAAGGGAACTTG+AGG	0.586339	3.3:+94948979	None:intergenic
AATGTAGCAAAAGCAGTTGG+TGG	0.597235	3.3:-94948863	MS.gene014273:CDS
CAAAATGTAGCAAAAGCAGT+TGG	0.599555	3.3:-94948866	MS.gene014273:CDS
GGAGTCCAAATTGAATCACA+AGG	0.603739	3.3:+94948953	None:intergenic
ATAAGGATACCCCTGACCGT+TGG	0.605106	3.3:-94948889	MS.gene014273:CDS
AGTTGGTGGTAAATCAGTTG+AGG	0.635333	3.3:-94948849	MS.gene014273:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCAAACAACTTGTTTTGTTT+TGG	+	chr3.3:94948814-94948833	None:intergenic	25.0%
	AGCACTTGCAAAATTTGATA+AGG	-	chr3.3:94948837-94948856	MS.gene014273:CDS	30.0%
!	GTGGATTTGTAATTTGGAAT+AGG	+	chr3.3:94948982-94949001	None:intergenic	30.0%
	CAAAATGTAGCAAAAGCAGT+TGG	-	chr3.3:94948877-94948896	MS.gene014273:CDS	35.0%
!	ACAAGCCTTGTGATTCAATT+TGG	-	chr3.3:94948785-94948804	MS.gene014273:CDS	35.0%
!	CTTTTGCTACATTTTGCCAA+CGG	+	chr3.3:94948873-94948892	None:intergenic	35.0%
	AATGTAGCAAAAGCAGTTGG+TGG	-	chr3.3:94948880-94948899	MS.gene014273:CDS	40.0%
	GATCTCAAACACATTGAGTC+TGG	-	chr3.3:94948949-94948968	MS.gene014273:CDS	40.0%
	GGAGTCCAAATTGAATCACA+AGG	+	chr3.3:94948793-94948812	None:intergenic	40.0%
!	ACAAGGCTTGTGTTTGCTAA+GGG	+	chr3.3:94948776-94948795	None:intergenic	40.0%
!!	AGTTGGTGGTAAATCAGTTG+AGG	-	chr3.3:94948894-94948913	MS.gene014273:CDS	40.0%
	ACACTATGAGCTACTCTTGG+AGG	-	chr3.3:94948927-94948946	MS.gene014273:CDS	45.0%
	GAGACACTATGAGCTACTCT+TGG	-	chr3.3:94948924-94948943	MS.gene014273:CDS	45.0%
!	CACAAGGCTTGTGTTTGCTA+AGG	+	chr3.3:94948777-94948796	None:intergenic	45.0%
!	CTACATTTTGCCAACGGTCA+GGG	+	chr3.3:94948867-94948886	None:intergenic	45.0%
!	GTGTTTGCTAAGGGAACTTG+AGG	+	chr3.3:94948767-94948786	None:intergenic	45.0%
!	TACATTTTGCCAACGGTCAG+GGG	+	chr3.3:94948866-94948885	None:intergenic	45.0%
	ATAAGGATACCCCTGACCGT+TGG	-	chr3.3:94948854-94948873	MS.gene014273:CDS	50.0%
!	GCTACATTTTGCCAACGGTC+AGG	+	chr3.3:94948868-94948887	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	94948760	94949005	94948760	ID=MS.gene014273
chr3.3	mRNA	94948760	94949005	94948760	ID=MS.gene014273.t1;Parent=MS.gene014273
chr3.3	exon	94948760	94949005	94948760	ID=MS.gene014273.t1.exon1;Parent=MS.gene014273.t1
chr3.3	CDS	94948760	94949005	94948760	ID=cds.MS.gene014273.t1;Parent=MS.gene014273.t1

Gene Sequence	>MS.gene014273 ATGGCCTCAAGTTCCCTTAGCAAACACAAGCCTTGTGATTCAATTTGGACTCCAAAACAAAACAAGTTGTTTGAAAAAGCACTTGCAAAATTTGATAAGGATACCCCTGACCGTTGGCAAAATGTAGCAAAAGCAGTTGGTGGTAAATCAGTTGAGGAAGTTAAGAGACACTATGAGCTACTCTTGGAGGATCTCAAACACATTGAGTCTGGTCATGTTCCTATTCCAAATTACAAATCCACGTGA
Protein sequence	>MS.gene014273.t1 MASSSLSKHKPCDSIWTPKQNKLFEKALAKFDKDTPDRWQNVAKAVGGKSVEEVKRHYELLLEDLKHIESGHVPIPNYKST