Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014346.t1 | XP_003629519.1 | 83.3 | 78 | 11 | 1 | 1 | 78 | 177 | 252 | 2.60E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014346.t1 | Q8W4F0 | 69.2 | 78 | 22 | 1 | 1 | 78 | 206 | 281 | 4.4e-29 | 128.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014346.t1 | G7LFK8 | 83.3 | 78 | 11 | 1 | 1 | 78 | 177 | 252 | 1.9e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014346.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014346.t1 | MTR_8g078410 | 84.416 | 77 | 10 | 1 | 1 | 77 | 177 | 251 | 2.71e-40 | 139 |
| MS.gene014346.t1 | MTR_5g018900 | 66.265 | 83 | 20 | 2 | 1 | 80 | 174 | 251 | 3.07e-33 | 119 |
| MS.gene014346.t1 | MTR_1g021835 | 60.000 | 85 | 28 | 2 | 1 | 81 | 127 | 209 | 4.39e-28 | 103 |
| MS.gene014346.t1 | MTR_1g021835 | 60.000 | 85 | 28 | 2 | 1 | 81 | 127 | 209 | 1.07e-27 | 104 |
| MS.gene014346.t1 | MTR_1g021835 | 60.000 | 85 | 28 | 2 | 1 | 81 | 127 | 209 | 1.38e-27 | 103 |
| MS.gene014346.t1 | MTR_5g081160 | 54.545 | 88 | 28 | 3 | 1 | 81 | 159 | 241 | 6.02e-24 | 94.0 |
| MS.gene014346.t1 | MTR_5g018910 | 72.093 | 43 | 12 | 0 | 1 | 43 | 514 | 556 | 5.37e-21 | 85.9 |
| MS.gene014346.t1 | MTR_3g069410 | 53.086 | 81 | 32 | 2 | 5 | 81 | 165 | 243 | 6.16e-21 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014346.t1 | AT4G36860 | 70.130 | 77 | 21 | 1 | 1 | 77 | 206 | 280 | 2.25e-32 | 117 |
| MS.gene014346.t1 | AT4G36860 | 70.130 | 77 | 21 | 1 | 1 | 77 | 206 | 280 | 3.25e-32 | 117 |
| MS.gene014346.t1 | AT4G36860 | 70.130 | 77 | 21 | 1 | 1 | 77 | 230 | 304 | 4.00e-32 | 117 |
| MS.gene014346.t1 | AT1G19270 | 55.844 | 77 | 32 | 1 | 1 | 77 | 188 | 262 | 6.90e-26 | 99.8 |
| MS.gene014346.t1 | AT1G19270 | 55.844 | 77 | 32 | 1 | 1 | 77 | 188 | 262 | 6.90e-26 | 99.8 |
| MS.gene014346.t1 | AT2G39830 | 57.143 | 77 | 31 | 1 | 1 | 77 | 169 | 243 | 4.10e-24 | 94.7 |
| MS.gene014346.t1 | AT2G39830 | 57.143 | 77 | 31 | 1 | 1 | 77 | 178 | 252 | 4.56e-24 | 94.0 |
| MS.gene014346.t1 | AT2G39830 | 57.143 | 77 | 31 | 1 | 1 | 77 | 178 | 252 | 4.56e-24 | 94.4 |
| MS.gene014346.t1 | AT2G39830 | 57.143 | 77 | 31 | 1 | 1 | 77 | 178 | 252 | 5.11e-24 | 94.4 |
| MS.gene014346.t1 | AT5G17890 | 48.684 | 76 | 35 | 3 | 2 | 77 | 1257 | 1328 | 3.29e-17 | 75.1 |
| MS.gene014346.t1 | AT5G66620 | 37.975 | 79 | 34 | 3 | 1 | 76 | 302 | 368 | 1.61e-12 | 61.6 |
| MS.gene014346.t1 | AT5G66620 | 37.975 | 79 | 34 | 3 | 1 | 76 | 293 | 359 | 3.38e-12 | 60.8 |
| MS.gene014346.t1 | AT5G66620 | 37.975 | 79 | 34 | 3 | 1 | 76 | 293 | 359 | 3.38e-12 | 60.8 |
| MS.gene014346.t1 | AT5G66620 | 37.975 | 79 | 34 | 3 | 1 | 76 | 293 | 359 | 5.01e-12 | 60.5 |
Find 20 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCCTTATAACATGATTTA+TGG | 0.141047 | 3.2:+91142581 | None:intergenic |
| AGTATAGAGCACATCCTTTC+TGG | 0.193526 | 3.2:-91141960 | MS.gene014346:CDS |
| TTTCTTAAATCGAGTCTTCT+TGG | 0.283213 | 3.2:+91141896 | None:intergenic |
| GTGATGAATCCAAGTATTAT+TGG | 0.304104 | 3.2:+91142550 | None:intergenic |
| CGAATCCGCATTGTGTTTGA+TGG | 0.355236 | 3.2:+91141922 | None:intergenic |
| TCATCACAGTATTCCATGTA+TGG | 0.390272 | 3.2:-91142616 | MS.gene014346:intron |
| TTACCATAAATCATGTTATA+AGG | 0.397268 | 3.2:-91142584 | MS.gene014346:CDS |
| CTGCATCACCCAATAATACT+TGG | 0.432723 | 3.2:-91142559 | MS.gene014346:CDS |
| TGATGAATCCAAGTATTATT+GGG | 0.446443 | 3.2:+91142551 | None:intergenic |
| CAAACCTCGTAATCAGTGAT+TGG | 0.457824 | 3.2:+91142779 | None:intergenic |
| TTATAACATGATTTATGGTA+AGG | 0.472109 | 3.2:+91142586 | None:intergenic |
| TGATTGGAAGTTTGCAAGCA+TGG | 0.482821 | 3.2:+91142795 | None:intergenic |
| TGAGTTGCATGGGAGCTGTA+TGG | 0.499390 | 3.2:-91142838 | None:intergenic |
| CTTAAATCGAGTCTTCTTGG+AGG | 0.533542 | 3.2:+91141899 | None:intergenic |
| GTAAGGACGATTCCCATACA+TGG | 0.543246 | 3.2:+91142603 | None:intergenic |
| CATCACAGTATTCCATGTAT+GGG | 0.556558 | 3.2:-91142615 | MS.gene014346:intron |
| ACTTCCAATCACTGATTACG+AGG | 0.616089 | 3.2:-91142783 | MS.gene014346:intron |
| GATGTGCTCTATACTCAATG+AGG | 0.649586 | 3.2:+91141968 | None:intergenic |
| TGGATTCATCACTGATTACG+AGG | 0.654224 | 3.2:-91142539 | MS.gene014346:intron |
| ATTGTCCATCAAACACAATG+CGG | 0.706834 | 3.2:-91141927 | MS.gene014346:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTAATATATTATATAAA+GGG | + | chr3.2:91142701-91142720 | None:intergenic | 0.0% |
| !!! | ATTTCTTAGATTTTAATAAT+TGG | - | chr3.2:91142522-91142541 | MS.gene014346:intron | 10.0% |
| !!! | TTTCTTAGATTTTAATAATT+GGG | - | chr3.2:91142523-91142542 | MS.gene014346:intron | 10.0% |
| !!! | TTTTCAAAATTACTTTTAGA+AGG | - | chr3.2:91142383-91142402 | MS.gene014346:intron | 15.0% |
| !! | AACATGTATTAGTATGTATT+AGG | - | chr3.2:91142491-91142510 | MS.gene014346:intron | 20.0% |
| !! | ACATGATTGTAAATAATACT+TGG | - | chr3.2:91142089-91142108 | MS.gene014346:intron | 20.0% |
| !! | ATGTTAACAACAAATAATCA+AGG | + | chr3.2:91142073-91142092 | None:intergenic | 20.0% |
| !! | TACTTATTTAAACTTTCCTT+AGG | - | chr3.2:91142024-91142043 | MS.gene014346:intron | 20.0% |
| !! | TTACCATAAATCATGTTATA+AGG | - | chr3.2:91142146-91142165 | MS.gene014346:intron | 20.0% |
| !! | TTATAACATGATTTATGGTA+AGG | + | chr3.2:91142147-91142166 | None:intergenic | 20.0% |
| ! | GTTAAACAAAATCATTCCTA+AGG | + | chr3.2:91142043-91142062 | None:intergenic | 25.0% |
| ! | TATAGAAATAACCAGTTCTT+AGG | - | chr3.2:91142659-91142678 | MS.gene014346:intron | 25.0% |
| ! | TGAAAAGAGATTAAAGTCAA+TGG | + | chr3.2:91142369-91142388 | None:intergenic | 25.0% |
| ! | TGATGAATCCAAGTATTATT+GGG | + | chr3.2:91142100-91142119 | None:intergenic | 25.0% |
| !!! | CAAAACATTATTTTGCACAA+AGG | + | chr3.2:91142432-91142451 | None:intergenic | 25.0% |
| !!! | TAATGTTTTGTTCTTTTCAC+AGG | - | chr3.2:91142442-91142461 | MS.gene014346:intron | 25.0% |
| AAATTTCACTACTCTATGTG+AGG | - | chr3.2:91142339-91142358 | MS.gene014346:intron | 30.0% | |
| CGCATAGTATTGTAAAATCA+GGG | + | chr3.2:91142608-91142627 | None:intergenic | 30.0% | |
| GCTCCTTATAACATGATTTA+TGG | + | chr3.2:91142152-91142171 | None:intergenic | 30.0% | |
| GTGATGAATCCAAGTATTAT+TGG | + | chr3.2:91142101-91142120 | None:intergenic | 30.0% | |
| TCGCATAGTATTGTAAAATC+AGG | + | chr3.2:91142609-91142628 | None:intergenic | 30.0% | |
| !! | CAATATTTTTGTAGCCAGAA+AGG | + | chr3.2:91142787-91142806 | None:intergenic | 30.0% |
| !! | TTTGTCAATTTTGTTGTGCT+TGG | - | chr3.2:91142277-91142296 | MS.gene014346:intron | 30.0% |
| ATTGTCCATCAAACACAATG+CGG | - | chr3.2:91142803-91142822 | MS.gene014346:CDS | 35.0% | |
| CATCACAGTATTCCATGTAT+GGG | - | chr3.2:91142115-91142134 | MS.gene014346:intron | 35.0% | |
| TAATGTCGTAACCTAAGAAC+TGG | + | chr3.2:91142673-91142692 | None:intergenic | 35.0% | |
| TCATCACAGTATTCCATGTA+TGG | - | chr3.2:91142114-91142133 | MS.gene014346:intron | 35.0% | |
| ! | TTATGATTTGCCAGAAGTTG+TGG | - | chr3.2:91142546-91142565 | MS.gene014346:CDS | 35.0% |
| AGTATAGAGCACATCCTTTC+TGG | - | chr3.2:91142770-91142789 | MS.gene014346:intron | 40.0% | |
| CAAACCTCGTAATCAGTGAT+TGG | + | chr3.2:91141954-91141973 | None:intergenic | 40.0% | |
| CTGCATCACCCAATAATACT+TGG | - | chr3.2:91142171-91142190 | MS.gene014346:intron | 40.0% | |
| GATGTGCTCTATACTCAATG+AGG | + | chr3.2:91142765-91142784 | None:intergenic | 40.0% | |
| GCATGGCAACAAAAACATTC+TGG | + | chr3.2:91141921-91141940 | None:intergenic | 40.0% | |
| TAGCGTTCAACAACGAAAAC+AGG | + | chr3.2:91141980-91141999 | None:intergenic | 40.0% | |
| TGATTGGAAGTTTGCAAGCA+TGG | + | chr3.2:91141938-91141957 | None:intergenic | 40.0% | |
| TGCTATTGATCCACAACTTC+TGG | + | chr3.2:91142559-91142578 | None:intergenic | 40.0% | |
| TTTGTGGGATCTGCAAAGAA+AGG | + | chr3.2:91142732-91142751 | None:intergenic | 40.0% | |
| ! | ACTTCCAATCACTGATTACG+AGG | - | chr3.2:91141947-91141966 | MS.gene014346:CDS | 40.0% |
| ! | TGGATTCATCACTGATTACG+AGG | - | chr3.2:91142191-91142210 | MS.gene014346:intron | 40.0% |
| CAGATCCCACAAAAATCAGC+TGG | - | chr3.2:91142739-91142758 | MS.gene014346:intron | 45.0% | |
| CGAATCCGCATTGTGTTTGA+TGG | + | chr3.2:91142811-91142830 | None:intergenic | 45.0% | |
| GTAAGGACGATTCCCATACA+TGG | + | chr3.2:91142130-91142149 | None:intergenic | 45.0% | |
| !! | ATGAGGCCAGCTGATTTTTG+TGG | + | chr3.2:91142748-91142767 | None:intergenic | 45.0% |
| !! | TGAGGCCAGCTGATTTTTGT+GGG | + | chr3.2:91142747-91142766 | None:intergenic | 45.0% |
| !! | GATATTAATATATTATATAA+AGG | + | chr3.2:91142702-91142721 | None:intergenic | 5.0% |
| !! | TATTAATATATTATATAAAG+GGG | + | chr3.2:91142700-91142719 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 91141900 | 91142852 | 91141900 | ID=MS.gene014346 |
| chr3.2 | mRNA | 91141900 | 91142852 | 91141900 | ID=MS.gene014346.t1;Parent=MS.gene014346 |
| chr3.2 | exon | 91142784 | 91142852 | 91142784 | ID=MS.gene014346.t1.exon1;Parent=MS.gene014346.t1 |
| chr3.2 | CDS | 91142784 | 91142852 | 91142784 | ID=cds.MS.gene014346.t1;Parent=MS.gene014346.t1 |
| chr3.2 | exon | 91142540 | 91142629 | 91142540 | ID=MS.gene014346.t1.exon2;Parent=MS.gene014346.t1 |
| chr3.2 | CDS | 91142540 | 91142629 | 91142540 | ID=cds.MS.gene014346.t1;Parent=MS.gene014346.t1 |
| chr3.2 | exon | 91141900 | 91142010 | 91141900 | ID=MS.gene014346.t1.exon3;Parent=MS.gene014346.t1 |
| chr3.2 | CDS | 91141900 | 91142010 | 91141900 | ID=cds.MS.gene014346.t1;Parent=MS.gene014346.t1 |
| Gene Sequence |
| Protein sequence |
