Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014360.t1 | XP_024632959.1 | 51.5 | 330 | 83 | 7 | 1 | 253 | 1 | 330 | 3.00E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014360.t1 | Q9SSW1 | 39.1 | 92 | 53 | 1 | 46 | 137 | 98 | 186 | 1.9e-08 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014360.t1 | A0A396JGH2 | 51.5 | 330 | 83 | 7 | 1 | 253 | 1 | 330 | 2.2e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014360.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014360.t1 | MTR_6g081160 | 33.788 | 293 | 114 | 8 | 20 | 253 | 1 | 272 | 1.11e-32 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014360.t1 | AT3G19580 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.94e-13 | 68.6 |
| MS.gene014360.t1 | AT3G19580 | 40.206 | 97 | 39 | 4 | 45 | 137 | 106 | 187 | 1.94e-13 | 68.6 |
| MS.gene014360.t1 | AT1G49900 | 39.130 | 92 | 38 | 2 | 46 | 137 | 194 | 267 | 1.58e-11 | 64.3 |
| MS.gene014360.t1 | AT5G67450 | 39.175 | 97 | 48 | 2 | 45 | 137 | 97 | 186 | 4.62e-11 | 61.6 |
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATTTCTCTTTACCAAAA+TGG | 0.235457 | 3.2:-91030803 | MS.gene014360:CDS |
| AAGCATGAGGTTATTATTAC+AGG | 0.279433 | 3.2:-91031221 | MS.gene014360:CDS |
| TCTAACTTTATCTTCTTAAG+AGG | 0.301299 | 3.2:+91031122 | None:intergenic |
| TCCGACTCTTCATAAGTATT+CGG | 0.323788 | 3.2:+91031074 | None:intergenic |
| CCTCTTGTCAGACTCTTCAT+AGG | 0.349643 | 3.2:+91031289 | None:intergenic |
| CATAAAGAAGATCAAGCTTA+TGG | 0.366706 | 3.2:-91030825 | MS.gene014360:CDS |
| TTACGAATAACATGATTTGC+TGG | 0.441171 | 3.2:+91030888 | None:intergenic |
| GAATGAGCCATATGCTATTG+AGG | 0.477019 | 3.2:-91030589 | MS.gene014360:CDS |
| AGAAGAGGTAGAAAGTGTAT+TGG | 0.483023 | 3.2:-91030768 | MS.gene014360:CDS |
| GCTTAGTCCTCAATAGCATA+TGG | 0.486464 | 3.2:+91030582 | None:intergenic |
| TTGGTGAGTCATCATCAAAT+TGG | 0.494071 | 3.2:-91030689 | MS.gene014360:CDS |
| CTAACTTTATCTTCTTAAGA+GGG | 0.512429 | 3.2:+91031123 | None:intergenic |
| TGAGATCTCCGGTGAGGAAG+GGG | 0.514342 | 3.2:+91031097 | None:intergenic |
| ACTGATGATCAGCTAAAGAA+AGG | 0.524124 | 3.2:-91030630 | MS.gene014360:CDS |
| GTGAGATCTCCGGTGAGGAA+GGG | 0.530993 | 3.2:+91031096 | None:intergenic |
| TCTTAATAGAGTGAGATCTC+TGG | 0.533566 | 3.2:+91030925 | None:intergenic |
| TAAGTATTCGGTGAGATCTC+CGG | 0.541580 | 3.2:+91031086 | None:intergenic |
| TTTAGCAGCGGACAAGCACT+TGG | 0.544373 | 3.2:-91031164 | MS.gene014360:CDS |
| CTTATGAAGAGTCGGAAAAG+AGG | 0.546986 | 3.2:-91031067 | MS.gene014360:CDS |
| TCTTCTTTATGATGTTCAGT+AGG | 0.559242 | 3.2:+91030837 | None:intergenic |
| GGTGAGATCTCCGGTGAGGA+AGG | 0.559786 | 3.2:+91031095 | None:intergenic |
| GTTAGAAACCCCTTCCTCAC+CGG | 0.572034 | 3.2:-91031105 | MS.gene014360:CDS |
| ACCGAATACTTATGAAGAGT+CGG | 0.572679 | 3.2:-91031075 | MS.gene014360:CDS |
| GAGTCATCATCAAATTGGAA+TGG | 0.587360 | 3.2:-91030684 | MS.gene014360:CDS |
| TGGCAAAAGAAAGATAGAAG+AGG | 0.598017 | 3.2:-91030783 | MS.gene014360:CDS |
| AGCAGCGGACAAGCACTTGG+TGG | 0.609210 | 3.2:-91031161 | MS.gene014360:CDS |
| TCTTGTTCACTATCAAGTGA+CGG | 0.609728 | 3.2:-91031254 | MS.gene014360:CDS |
| TGGAATGGCAAGAATGAGCT+TGG | 0.611635 | 3.2:-91030669 | MS.gene014360:CDS |
| CCTATGAAGAGTCTGACAAG+AGG | 0.641227 | 3.2:-91031289 | MS.gene014360:CDS |
| CGAAGATGATGTCAAGCATG+AGG | 0.647694 | 3.2:-91031012 | MS.gene014360:CDS |
| CGGAGATGATGCAAAGCATG+AGG | 0.655546 | 3.2:-91031234 | MS.gene014360:CDS |
| GTGTATTGGTAGTGTTGAAG+CGG | 0.655661 | 3.2:-91030754 | MS.gene014360:CDS |
| AAAAGTACTTATCTGAACGA+AGG | 0.659168 | 3.2:+91030715 | None:intergenic |
| AATGGCAAGAATGAGCTTGG+TGG | 0.663111 | 3.2:-91030666 | MS.gene014360:CDS |
| ATTCGGTGAGATCTCCGGTG+AGG | 0.731513 | 3.2:+91031091 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCAGAAAAACTTTTAAAAA+TGG | - | chr3.2:91030941-91030960 | MS.gene014360:CDS | 20.0% |
| !!! | TTTAAAAATGGAAAAGCATT+GGG | - | chr3.2:91030953-91030972 | MS.gene014360:CDS | 20.0% |
| !!! | TTTTAAAAATGGAAAAGCAT+TGG | - | chr3.2:91030952-91030971 | MS.gene014360:CDS | 20.0% |
| ! | CTAACTTTATCTTCTTAAGA+GGG | + | chr3.2:91030787-91030806 | None:intergenic | 25.0% |
| ! | GTAATTTCTCTTTACCAAAA+TGG | - | chr3.2:91031104-91031123 | MS.gene014360:CDS | 25.0% |
| ! | TCTAACTTTATCTTCTTAAG+AGG | + | chr3.2:91030788-91030807 | None:intergenic | 25.0% |
| AAGCATGAGGTTATTATTAC+AGG | - | chr3.2:91030686-91030705 | MS.gene014360:CDS | 30.0% | |
| CATAAAGAAGATCAAGCTTA+TGG | - | chr3.2:91031082-91031101 | MS.gene014360:CDS | 30.0% | |
| TCTTCTTTATGATGTTCAGT+AGG | + | chr3.2:91031073-91031092 | None:intergenic | 30.0% | |
| TGTGAAAAAGAATTTAGCAG+CGG | - | chr3.2:91030731-91030750 | MS.gene014360:CDS | 30.0% | |
| ! | GTTTTCTCATAATCATGCTT+AGG | + | chr3.2:91030599-91030618 | None:intergenic | 30.0% |
| ! | TCAGATAAGTACTTTTGTGT+TGG | - | chr3.2:91031199-91031218 | MS.gene014360:CDS | 30.0% |
| ! | TTACGAATAACATGATTTGC+TGG | + | chr3.2:91031022-91031041 | None:intergenic | 30.0% |
| ! | TTTTTCTGCAAATATTGCAC+TGG | + | chr3.2:91030931-91030950 | None:intergenic | 30.0% |
| !! | AAAAGTACTTATCTGAACGA+AGG | + | chr3.2:91031195-91031214 | None:intergenic | 30.0% |
| !! | CTATCTTTCTTTTGCCATTT+TGG | + | chr3.2:91031121-91031140 | None:intergenic | 30.0% |
| ACCGAATACTTATGAAGAGT+CGG | - | chr3.2:91030832-91030851 | MS.gene014360:CDS | 35.0% | |
| ACTGATGATCAGCTAAAGAA+AGG | - | chr3.2:91031277-91031296 | MS.gene014360:CDS | 35.0% | |
| GAGTCATCATCAAATTGGAA+TGG | - | chr3.2:91031223-91031242 | MS.gene014360:CDS | 35.0% | |
| TCCGACTCTTCATAAGTATT+CGG | + | chr3.2:91030836-91030855 | None:intergenic | 35.0% | |
| TCTTAATAGAGTGAGATCTC+TGG | + | chr3.2:91030985-91031004 | None:intergenic | 35.0% | |
| TCTTGTTCACTATCAAGTGA+CGG | - | chr3.2:91030653-91030672 | MS.gene014360:CDS | 35.0% | |
| TGGCAAAAGAAAGATAGAAG+AGG | - | chr3.2:91031124-91031143 | MS.gene014360:CDS | 35.0% | |
| TTGGTGAGTCATCATCAAAT+TGG | - | chr3.2:91031218-91031237 | MS.gene014360:CDS | 35.0% | |
| ! | AGAAGAGGTAGAAAGTGTAT+TGG | - | chr3.2:91031139-91031158 | MS.gene014360:CDS | 35.0% |
| !! | AAAAATGGAAAAGCATTGGG+TGG | - | chr3.2:91030956-91030975 | MS.gene014360:CDS | 35.0% |
| CTTATGAAGAGTCGGAAAAG+AGG | - | chr3.2:91030840-91030859 | MS.gene014360:CDS | 40.0% | |
| GAATGAGCCATATGCTATTG+AGG | - | chr3.2:91031318-91031337 | MS.gene014360:CDS | 40.0% | |
| ! | TAAGTATTCGGTGAGATCTC+CGG | + | chr3.2:91030824-91030843 | None:intergenic | 40.0% |
| !! | GTGTATTGGTAGTGTTGAAG+CGG | - | chr3.2:91031153-91031172 | MS.gene014360:CDS | 40.0% |
| AATGGCAAGAATGAGCTTGG+TGG | - | chr3.2:91031241-91031260 | MS.gene014360:CDS | 45.0% | |
| CCTATGAAGAGTCTGACAAG+AGG | - | chr3.2:91030618-91030637 | MS.gene014360:CDS | 45.0% | |
| CCTCTTGTCAGACTCTTCAT+AGG | + | chr3.2:91030621-91030640 | None:intergenic | 45.0% | |
| CGAAGATGATGTCAAGCATG+AGG | - | chr3.2:91030895-91030914 | MS.gene014360:CDS | 45.0% | |
| TGGAATGGCAAGAATGAGCT+TGG | - | chr3.2:91031238-91031257 | MS.gene014360:CDS | 45.0% | |
| CGGAGATGATGCAAAGCATG+AGG | - | chr3.2:91030673-91030692 | MS.gene014360:CDS | 50.0% | |
| GTTAGAAACCCCTTCCTCAC+CGG | - | chr3.2:91030802-91030821 | MS.gene014360:CDS | 50.0% | |
| TTTAGCAGCGGACAAGCACT+TGG | - | chr3.2:91030743-91030762 | MS.gene014360:CDS | 50.0% | |
| GTGAGATCTCCGGTGAGGAA+GGG | + | chr3.2:91030814-91030833 | None:intergenic | 55.0% | |
| TGAGATCTCCGGTGAGGAAG+GGG | + | chr3.2:91030813-91030832 | None:intergenic | 55.0% | |
| ! | ATTCGGTGAGATCTCCGGTG+AGG | + | chr3.2:91030819-91030838 | None:intergenic | 55.0% |
| AGCAGCGGACAAGCACTTGG+TGG | - | chr3.2:91030746-91030765 | MS.gene014360:CDS | 60.0% | |
| GGTGAGATCTCCGGTGAGGA+AGG | + | chr3.2:91030815-91030834 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 91030584 | 91031345 | 91030584 | ID=MS.gene014360 |
| chr3.2 | mRNA | 91030584 | 91031345 | 91030584 | ID=MS.gene014360.t1;Parent=MS.gene014360 |
| chr3.2 | exon | 91030584 | 91031345 | 91030584 | ID=MS.gene014360.t1.exon1;Parent=MS.gene014360.t1 |
| chr3.2 | CDS | 91030584 | 91031345 | 91030584 | ID=cds.MS.gene014360.t1;Parent=MS.gene014360.t1 |
| Gene Sequence |
| Protein sequence |
