Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014515.t1 | XP_013462122.1 | 94.5 | 91 | 5 | 0 | 1 | 91 | 1 | 91 | 3.20E-42 | 180.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014515.t1 | F4JVB8 | 60.0 | 90 | 34 | 1 | 3 | 90 | 10 | 99 | 5.7e-24 | 111.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014515.t1 | A0A072V2R8 | 94.5 | 91 | 5 | 0 | 1 | 91 | 1 | 91 | 2.3e-42 | 180.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014515.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014515.t1 | MTR_3g111880 | 94.505 | 91 | 5 | 0 | 1 | 91 | 1 | 91 | 1.67e-62 | 184 |
| MS.gene014515.t1 | MTR_1g022290 | 74.468 | 94 | 20 | 2 | 1 | 91 | 1 | 93 | 2.57e-46 | 143 |
| MS.gene014515.t1 | MTR_3g116720 | 72.059 | 68 | 19 | 0 | 5 | 72 | 14 | 81 | 2.78e-33 | 110 |
| MS.gene014515.t1 | MTR_3g013440 | 57.609 | 92 | 34 | 2 | 1 | 91 | 4 | 91 | 2.26e-32 | 108 |
| MS.gene014515.t1 | MTR_7g089210 | 72.131 | 61 | 17 | 0 | 9 | 69 | 14 | 74 | 8.17e-27 | 94.4 |
| MS.gene014515.t1 | MTR_6g004250 | 57.143 | 70 | 30 | 0 | 2 | 71 | 5 | 74 | 4.05e-26 | 92.0 |
| MS.gene014515.t1 | MTR_8g077380 | 55.714 | 70 | 30 | 1 | 4 | 73 | 2 | 70 | 2.69e-25 | 90.1 |
| MS.gene014515.t1 | MTR_8g077390 | 55.714 | 70 | 30 | 1 | 4 | 73 | 2 | 70 | 2.69e-25 | 90.1 |
| MS.gene014515.t1 | MTR_8g077360 | 55.714 | 70 | 30 | 1 | 4 | 73 | 2 | 70 | 3.33e-25 | 89.7 |
| MS.gene014515.t1 | MTR_5g020170 | 61.194 | 67 | 26 | 0 | 3 | 69 | 15 | 81 | 2.26e-24 | 88.2 |
| MS.gene014515.t1 | MTR_8g077420 | 58.571 | 70 | 28 | 1 | 4 | 73 | 2 | 70 | 7.60e-24 | 86.3 |
| MS.gene014515.t1 | MTR_3g111920 | 52.113 | 71 | 34 | 0 | 6 | 76 | 2 | 72 | 1.39e-22 | 83.6 |
| MS.gene014515.t1 | MTR_5g020540 | 61.905 | 63 | 23 | 1 | 5 | 67 | 2 | 63 | 1.45e-22 | 82.8 |
| MS.gene014515.t1 | MTR_1g033600 | 56.522 | 69 | 30 | 0 | 4 | 72 | 9 | 77 | 1.83e-22 | 84.7 |
| MS.gene014515.t1 | MTR_5g069710 | 45.652 | 92 | 39 | 1 | 4 | 84 | 26 | 117 | 5.93e-21 | 84.3 |
| MS.gene014515.t1 | MTR_5g081860 | 55.385 | 65 | 29 | 0 | 5 | 69 | 25 | 89 | 6.58e-21 | 83.6 |
| MS.gene014515.t1 | MTR_8g063870 | 57.576 | 66 | 28 | 0 | 4 | 69 | 5 | 70 | 6.99e-21 | 83.6 |
| MS.gene014515.t1 | MTR_7g067080 | 50.000 | 78 | 36 | 1 | 5 | 82 | 33 | 107 | 3.32e-20 | 82.4 |
| MS.gene014515.t1 | MTR_8g063600 | 53.623 | 69 | 32 | 0 | 1 | 69 | 4 | 72 | 1.21e-19 | 80.5 |
| MS.gene014515.t1 | MTR_3g462790 | 53.731 | 67 | 31 | 0 | 4 | 70 | 40 | 106 | 4.27e-19 | 79.7 |
| MS.gene014515.t1 | MTR_4g111975 | 46.667 | 75 | 37 | 2 | 2 | 73 | 3 | 77 | 1.90e-17 | 74.7 |
| MS.gene014515.t1 | MTR_1g111830 | 46.269 | 67 | 36 | 0 | 5 | 71 | 22 | 88 | 5.40e-17 | 72.8 |
| MS.gene014515.t1 | MTR_1g086180 | 49.153 | 59 | 29 | 1 | 5 | 62 | 9 | 67 | 3.90e-14 | 62.4 |
| MS.gene014515.t1 | MTR_7g110580 | 49.206 | 63 | 31 | 1 | 3 | 64 | 4 | 66 | 6.19e-14 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014515.t1 | AT4G39250 | 59.770 | 87 | 33 | 1 | 7 | 91 | 14 | 100 | 1.36e-32 | 109 |
| MS.gene014515.t1 | AT2G21650 | 57.955 | 88 | 34 | 1 | 7 | 91 | 14 | 101 | 2.19e-31 | 106 |
| MS.gene014515.t1 | AT1G75250 | 72.059 | 68 | 19 | 0 | 2 | 69 | 7 | 74 | 5.47e-31 | 105 |
| MS.gene014515.t1 | AT1G75250 | 62.637 | 91 | 32 | 2 | 2 | 90 | 7 | 97 | 9.78e-31 | 104 |
| MS.gene014515.t1 | AT1G19510 | 68.182 | 66 | 20 | 1 | 10 | 74 | 15 | 80 | 1.11e-25 | 91.7 |
| MS.gene014515.t1 | AT2G18328 | 64.516 | 62 | 22 | 0 | 10 | 71 | 14 | 75 | 4.29e-25 | 89.7 |
| MS.gene014515.t1 | AT2G38090 | 52.174 | 69 | 33 | 0 | 4 | 72 | 26 | 94 | 5.29e-21 | 84.3 |
| MS.gene014515.t1 | AT5G58900 | 54.545 | 66 | 30 | 0 | 4 | 69 | 31 | 96 | 1.51e-20 | 83.2 |
| MS.gene014515.t1 | AT5G05790 | 47.436 | 78 | 39 | 1 | 4 | 79 | 28 | 105 | 1.49e-19 | 80.1 |
| MS.gene014515.t1 | AT5G05790 | 47.436 | 78 | 39 | 1 | 4 | 79 | 28 | 105 | 1.49e-19 | 80.1 |
| MS.gene014515.t1 | AT4G36570 | 72.727 | 44 | 12 | 0 | 7 | 50 | 12 | 55 | 2.74e-18 | 72.0 |
| MS.gene014515.t1 | AT3G11280 | 46.053 | 76 | 38 | 1 | 7 | 79 | 33 | 108 | 1.80e-17 | 74.7 |
| MS.gene014515.t1 | AT3G11280 | 46.053 | 76 | 38 | 1 | 7 | 79 | 33 | 108 | 1.80e-17 | 74.7 |
| MS.gene014515.t1 | AT5G08520 | 44.737 | 76 | 42 | 0 | 1 | 76 | 6 | 81 | 9.50e-16 | 70.5 |
| MS.gene014515.t1 | AT5G01200 | 34.951 | 103 | 49 | 2 | 5 | 89 | 28 | 130 | 1.37e-15 | 69.7 |
| MS.gene014515.t1 | AT1G49010 | 41.250 | 80 | 42 | 2 | 5 | 81 | 6 | 83 | 5.38e-14 | 65.5 |
| MS.gene014515.t1 | AT5G04760 | 40.299 | 67 | 39 | 1 | 3 | 69 | 2 | 67 | 6.49e-13 | 61.6 |
| MS.gene014515.t1 | AT5G23650 | 42.029 | 69 | 40 | 0 | 1 | 69 | 7 | 75 | 5.40e-12 | 60.1 |
Find 26 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACATTATTTGTGTAATT+AGG | 0.311673 | 3.1:-89325651 | None:intergenic |
| AGAACCTTGTTGAAGATATT+AGG | 0.315510 | 3.1:+89325601 | MS.gene014515:CDS |
| ACATAATGGATCAAGATAAA+AGG | 0.337165 | 3.1:+89325682 | MS.gene014515:CDS |
| TAATGGCTTCAAGTTCGAAT+TGG | 0.366111 | 3.1:+89325451 | None:intergenic |
| TGTTGGTTACAATTACATAA+TGG | 0.412132 | 3.1:+89325668 | MS.gene014515:CDS |
| CATTATTTGTGTAATTAGGC+AGG | 0.435090 | 3.1:-89325647 | None:intergenic |
| GCCACCTTCTGCCAACGATC+AGG | 0.467098 | 3.1:-89325531 | None:intergenic |
| TGAGAATGCGTTGGCAATGT+TGG | 0.488680 | 3.1:+89325497 | MS.gene014515:CDS |
| TGTAATTAGGCAGGGGAACA+TGG | 0.488710 | 3.1:-89325638 | None:intergenic |
| CCTAATTACACAAATAATGT+TGG | 0.495931 | 3.1:+89325651 | MS.gene014515:CDS |
| AGGCTCTAAGTCTCCACTAA+AGG | 0.510302 | 3.1:+89326173 | MS.gene014515:CDS |
| ATTATTTGTGTAATTAGGCA+GGG | 0.547965 | 3.1:-89325646 | None:intergenic |
| GCAGAAGGTGGCTAGAGCTG+TGG | 0.552998 | 3.1:+89325542 | MS.gene014515:CDS |
| CAAAAGATTTGAGAATGCGT+TGG | 0.553213 | 3.1:+89325488 | MS.gene014515:CDS |
| TCATGCATGATGCAGGATGA+AGG | 0.557115 | 3.1:+89326153 | MS.gene014515:intron |
| CAGAAGGTGGCTAGAGCTGT+GGG | 0.558074 | 3.1:+89325543 | MS.gene014515:CDS |
| CACTCCTGATCGTTGGCAGA+AGG | 0.576549 | 3.1:+89325527 | MS.gene014515:CDS |
| GATATTAGGCAGATCGAGGA+AGG | 0.589514 | 3.1:+89325615 | MS.gene014515:CDS |
| TTATTTGTGTAATTAGGCAG+GGG | 0.601636 | 3.1:-89325645 | None:intergenic |
| TCCTGATCGTTGGCAGAAGG+TGG | 0.602919 | 3.1:+89325530 | MS.gene014515:CDS |
| ACAAGGACACTCCTGATCGT+TGG | 0.625458 | 3.1:+89325520 | MS.gene014515:CDS |
| TGCGTTGGCAATGTTGGACA+AGG | 0.631324 | 3.1:+89325503 | MS.gene014515:CDS |
| TGAAGATATTAGGCAGATCG+AGG | 0.643314 | 3.1:+89325611 | MS.gene014515:CDS |
| AGGTGGCTAGAGCTGTGGGA+GGG | 0.662019 | 3.1:+89325547 | MS.gene014515:CDS |
| AAGGTGGCTAGAGCTGTGGG+AGG | 0.685328 | 3.1:+89325546 | MS.gene014515:CDS |
| TCTGCCTAATATCTTCAACA+AGG | 0.692316 | 3.1:-89325605 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTTATATGTTTATAAGT+AGG | + | chr3.1:89325945-89325964 | MS.gene014515:intron | 10.0% |
| !! | GATCAAATAAATAATATAAT+TGG | - | chr3.1:89326108-89326127 | None:intergenic | 10.0% |
| !! | TATATTATTTATTTGATCTC+TGG | + | chr3.1:89326110-89326129 | MS.gene014515:intron | 15.0% |
| !! | GATATTCTTAAAGTTAAGAT+TGG | - | chr3.1:89326015-89326034 | None:intergenic | 20.0% |
| !!! | ATTGCTGTTTTAATTGTTTT+TGG | - | chr3.1:89325883-89325902 | None:intergenic | 20.0% |
| !!! | TGATTTGTTGTAGATTAATA+TGG | + | chr3.1:89325775-89325794 | MS.gene014515:intron | 20.0% |
| !!! | TTGGTTTTATTCAAGTTTTT+TGG | - | chr3.1:89325864-89325883 | None:intergenic | 20.0% |
| !!! | TTTATTCAAGTTTTTTGGTT+TGG | - | chr3.1:89325859-89325878 | None:intergenic | 20.0% |
| ! | ATTATTTGTGTAATTAGGCA+GGG | - | chr3.1:89325649-89325668 | None:intergenic | 25.0% |
| ! | CCAACATTATTTGTGTAATT+AGG | - | chr3.1:89325654-89325673 | None:intergenic | 25.0% |
| ! | CCTAATTACACAAATAATGT+TGG | + | chr3.1:89325651-89325670 | MS.gene014515:CDS | 25.0% |
| ! | TGATTATTATTCATACATGC+AGG | + | chr3.1:89326066-89326085 | MS.gene014515:intron | 25.0% |
| ! | TGCATGTATGAATAATAATC+AGG | - | chr3.1:89326067-89326086 | None:intergenic | 25.0% |
| !! | TGTTGGTTACAATTACATAA+TGG | + | chr3.1:89325668-89325687 | MS.gene014515:CDS | 25.0% |
| !! | TTCATGTGTAACAAACATTT+TGG | - | chr3.1:89326041-89326060 | None:intergenic | 25.0% |
| !!! | ACATAATGGATCAAGATAAA+AGG | + | chr3.1:89325682-89325701 | MS.gene014515:CDS | 25.0% |
| AGAACCTTGTTGAAGATATT+AGG | + | chr3.1:89325601-89325620 | MS.gene014515:CDS | 30.0% | |
| CATTATTTGTGTAATTAGGC+AGG | - | chr3.1:89325650-89325669 | None:intergenic | 30.0% | |
| CTAGTTTCATTGTGTTTGAT+TGG | - | chr3.1:89325808-89325827 | None:intergenic | 30.0% | |
| TTATTTGTGTAATTAGGCAG+GGG | - | chr3.1:89325648-89325667 | None:intergenic | 30.0% | |
| ! | AAATCTTTTGTTCTGCTTTG+CGG | - | chr3.1:89325478-89325497 | None:intergenic | 30.0% |
| ! | ACTTTACATAACTAGCTTGA+AGG | - | chr3.1:89325983-89326002 | None:intergenic | 30.0% |
| !! | CATTGTGTTTGATTGGTTTA+AGG | - | chr3.1:89325801-89325820 | None:intergenic | 30.0% |
| CAAAAGATTTGAGAATGCGT+TGG | + | chr3.1:89325488-89325507 | MS.gene014515:CDS | 35.0% | |
| GTATTTATCATGCATGATGC+AGG | + | chr3.1:89326146-89326165 | MS.gene014515:intron | 35.0% | |
| TCTGCCTAATATCTTCAACA+AGG | - | chr3.1:89325608-89325627 | None:intergenic | 35.0% | |
| TGAAGATATTAGGCAGATCG+AGG | + | chr3.1:89325611-89325630 | MS.gene014515:CDS | 40.0% | |
| GATATTAGGCAGATCGAGGA+AGG | + | chr3.1:89325615-89325634 | MS.gene014515:CDS | 45.0% | |
| TCATGCATGATGCAGGATGA+AGG | + | chr3.1:89326153-89326172 | MS.gene014515:intron | 45.0% | |
| TGTAATTAGGCAGGGGAACA+TGG | - | chr3.1:89325641-89325660 | None:intergenic | 45.0% | |
| ! | TGAGAATGCGTTGGCAATGT+TGG | + | chr3.1:89325497-89325516 | MS.gene014515:CDS | 45.0% |
| !! | TAATATATATAAATTGAAAT+TGG | - | chr3.1:89325732-89325751 | None:intergenic | 5.0% |
| ACAAGGACACTCCTGATCGT+TGG | + | chr3.1:89325520-89325539 | MS.gene014515:CDS | 50.0% | |
| ! | TGCGTTGGCAATGTTGGACA+AGG | + | chr3.1:89325503-89325522 | MS.gene014515:CDS | 50.0% |
| CACTCCTGATCGTTGGCAGA+AGG | + | chr3.1:89325527-89325546 | MS.gene014515:CDS | 55.0% | |
| CAGAAGGTGGCTAGAGCTGT+GGG | + | chr3.1:89325543-89325562 | MS.gene014515:CDS | 55.0% | |
| TCCTGATCGTTGGCAGAAGG+TGG | + | chr3.1:89325530-89325549 | MS.gene014515:CDS | 55.0% | |
| AAGGTGGCTAGAGCTGTGGG+AGG | + | chr3.1:89325546-89325565 | MS.gene014515:CDS | 60.0% | |
| AGGTGGCTAGAGCTGTGGGA+GGG | + | chr3.1:89325547-89325566 | MS.gene014515:CDS | 60.0% | |
| GCAGAAGGTGGCTAGAGCTG+TGG | + | chr3.1:89325542-89325561 | MS.gene014515:CDS | 60.0% | |
| GCCACCTTCTGCCAACGATC+AGG | - | chr3.1:89325534-89325553 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 89325453 | 89326192 | 89325453 | ID=MS.gene014515 |
| chr3.1 | mRNA | 89325453 | 89326192 | 89325453 | ID=MS.gene014515.t1;Parent=MS.gene014515 |
| chr3.1 | exon | 89325453 | 89325703 | 89325453 | ID=MS.gene014515.t1.exon1;Parent=MS.gene014515.t1 |
| chr3.1 | CDS | 89325453 | 89325703 | 89325453 | ID=cds.MS.gene014515.t1;Parent=MS.gene014515.t1 |
| chr3.1 | exon | 89326168 | 89326192 | 89326168 | ID=MS.gene014515.t1.exon2;Parent=MS.gene014515.t1 |
| chr3.1 | CDS | 89326168 | 89326192 | 89326168 | ID=cds.MS.gene014515.t1;Parent=MS.gene014515.t1 |
| Gene Sequence |
| Protein sequence |
