AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01452

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01452.t1 MTR_2g014060 85.014 347 33 5 1 339 1 336 9.29e-163 457
MS.gene01452.t1 MTR_2g096740 64.444 90 26 2 12 95 45 134 2.02e-33 125
MS.gene01452.t1 MTR_2g096740 64.444 90 26 2 12 95 60 149 2.56e-33 125
MS.gene01452.t1 MTR_8g027295 64.706 85 29 1 19 102 17 101 2.36e-32 122
MS.gene01452.t1 MTR_8g068210 84.127 63 9 1 26 88 33 94 5.38e-31 120
MS.gene01452.t1 MTR_4g461080 76.471 68 13 2 11 76 14 80 5.75e-31 118
MS.gene01452.t1 MTR_7g024670 55.882 102 34 2 11 111 62 153 9.70e-31 120
MS.gene01452.t1 MTR_4g063780 70.423 71 20 1 22 91 71 141 5.62e-30 117
MS.gene01452.t1 MTR_2g093220 77.049 61 11 1 22 79 34 94 1.50e-29 115
MS.gene01452.t1 MTR_4g088580 64.706 85 19 2 26 100 56 139 1.54e-29 117
MS.gene01452.t1 MTR_3g091820 73.846 65 14 1 19 80 34 98 2.93e-29 114
MS.gene01452.t1 MTR_8g479350 82.143 56 10 0 23 78 66 121 4.53e-29 114
MS.gene01452.t1 MTR_3g090430 70.769 65 16 1 17 81 46 107 7.65e-29 114
MS.gene01452.t1 MTR_3g077750 81.818 55 10 0 21 75 57 111 8.54e-29 114
MS.gene01452.t1 MTR_5g031440 86.792 53 7 0 23 75 41 93 1.30e-28 113
MS.gene01452.t1 MTR_7g059400 64.865 74 25 1 6 79 58 130 1.63e-28 113
MS.gene01452.t1 MTR_1g077600 65.217 69 19 1 18 81 31 99 3.20e-28 110
MS.gene01452.t1 MTR_1g077600 65.217 69 19 1 18 81 31 99 4.07e-28 111
MS.gene01452.t1 MTR_4g022370 65.333 75 21 2 10 79 62 136 7.66e-28 112
MS.gene01452.t1 MTR_4g109980 86.000 50 7 0 26 75 41 90 8.76e-28 110
MS.gene01452.t1 MTR_1g056810 79.245 53 11 0 27 79 14 66 1.06e-26 107
MS.gene01452.t1 MTR_2g059540 85.417 48 7 0 26 73 32 79 1.47e-26 104
MS.gene01452.t1 MTR_8g079060 62.667 75 23 2 22 91 22 96 3.29e-26 104
MS.gene01452.t1 MTR_2g014170 87.500 48 6 0 26 73 46 93 1.59e-25 104
MS.gene01452.t1 MTR_8g015840 71.186 59 17 0 26 84 21 79 1.65e-25 102
MS.gene01452.t1 MTR_4g089095 74.545 55 13 1 27 81 52 105 5.43e-24 100
MS.gene01452.t1 MTR_4g089095 74.545 55 13 1 27 81 18 71 6.87e-24 99.0
MS.gene01452.t1 MTR_2g013370 62.857 70 23 2 14 81 33 101 7.50e-24 99.4
MS.gene01452.t1 MTR_3g435480 66.667 57 19 0 27 83 138 194 1.47e-23 101
MS.gene01452.t1 MTR_7g010950 70.370 54 16 0 27 80 137 190 2.01e-23 100
MS.gene01452.t1 MTR_4g082060 66.667 57 19 0 27 83 124 180 6.38e-23 99.4
MS.gene01452.t1 MTR_7g086780 54.167 72 33 0 7 78 81 152 7.14e-23 99.0
MS.gene01452.t1 MTR_6g012450 52.273 88 33 2 17 95 125 212 1.44e-22 98.6
MS.gene01452.t1 MTR_8g044220 66.667 54 18 0 27 80 120 173 1.68e-22 97.8
MS.gene01452.t1 MTR_2g016030 69.231 52 16 0 27 78 49 100 2.42e-22 92.4
MS.gene01452.t1 MTR_6g027450 64.815 54 19 0 26 79 90 143 4.25e-22 95.5
MS.gene01452.t1 MTR_6g027460 66.038 53 18 0 26 78 97 149 4.71e-22 95.9
MS.gene01452.t1 MTR_5g041420 54.545 77 31 1 4 80 121 193 1.15e-20 91.3
MS.gene01452.t1 MTR_5g041380 62.712 59 22 0 27 85 129 187 8.51e-20 89.7
MS.gene01452.t1 MTR_5g041530 65.385 52 18 0 27 78 142 193 1.16e-19 89.4
MS.gene01452.t1 MTR_5g041400 62.712 59 22 0 27 85 121 179 2.10e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01452.t1 AT5G60850 61.957 92 29 2 18 109 44 129 1.19e-32 124
MS.gene01452.t1 AT5G60200 72.059 68 15 1 22 85 48 115 6.21e-32 120
MS.gene01452.t1 AT5G62940 73.913 69 13 1 7 75 60 123 1.56e-31 122
MS.gene01452.t1 AT2G28510 75.806 62 15 0 23 84 46 107 2.33e-30 117
MS.gene01452.t1 AT1G51700 68.919 74 20 2 26 99 34 104 1.37e-29 112
MS.gene01452.t1 AT1G07640 66.216 74 23 1 8 79 2 75 1.43e-29 114
MS.gene01452.t1 AT5G65590 77.273 66 15 0 15 80 31 96 1.74e-29 115
MS.gene01452.t1 AT1G07640 66.216 74 23 1 8 79 58 131 2.33e-29 115
MS.gene01452.t1 AT1G07640 66.216 74 23 1 8 79 66 139 2.61e-29 115
MS.gene01452.t1 AT3G61850 61.176 85 30 2 19 102 55 137 2.64e-29 114
MS.gene01452.t1 AT3G61850 61.176 85 30 2 19 102 55 137 2.64e-29 114
MS.gene01452.t1 AT3G61850 61.176 85 30 2 19 102 67 149 3.50e-29 114
MS.gene01452.t1 AT3G52440 71.212 66 19 0 27 92 27 92 3.94e-29 112
MS.gene01452.t1 AT3G61850 61.176 85 30 2 19 102 55 137 4.14e-29 114
MS.gene01452.t1 AT3G52440 71.212 66 19 0 27 92 46 111 4.85e-29 113
MS.gene01452.t1 AT3G61850 61.176 85 30 2 19 102 67 149 5.01e-29 114
MS.gene01452.t1 AT3G45610 70.312 64 19 0 23 86 38 101 6.20e-29 112
MS.gene01452.t1 AT2G28810 63.380 71 26 0 6 76 58 128 1.11e-28 113
MS.gene01452.t1 AT2G37590 76.786 56 13 0 26 81 90 145 1.29e-28 113
MS.gene01452.t1 AT2G28810 63.380 71 26 0 6 76 75 145 1.54e-28 113
MS.gene01452.t1 AT4G21050 66.176 68 23 0 22 89 21 88 2.75e-28 109
MS.gene01452.t1 AT3G55370 75.000 60 15 0 27 86 78 137 3.39e-28 112
MS.gene01452.t1 AT3G55370 75.000 60 15 0 27 86 78 137 4.24e-28 112
MS.gene01452.t1 AT5G66940 49.091 110 51 3 27 131 34 143 4.78e-28 109
MS.gene01452.t1 AT3G55370 64.384 73 23 1 17 86 110 182 5.98e-28 112
MS.gene01452.t1 AT1G21340 75.862 58 14 0 27 84 40 97 1.02e-27 109
MS.gene01452.t1 AT1G28310 80.357 56 11 0 26 81 28 83 1.03e-27 110
MS.gene01452.t1 AT1G28310 80.357 56 11 0 26 81 52 107 1.56e-27 110
MS.gene01452.t1 AT4G00940 75.439 57 14 0 23 79 66 122 1.64e-27 109
MS.gene01452.t1 AT4G00940 75.439 57 14 0 23 79 66 122 1.64e-27 109
MS.gene01452.t1 AT4G00940 75.439 57 14 0 23 79 66 122 1.64e-27 109
MS.gene01452.t1 AT5G02460 59.783 92 36 1 26 117 96 186 3.10e-27 110
MS.gene01452.t1 AT3G21270 84.314 51 8 0 26 76 30 80 1.37e-26 105
MS.gene01452.t1 AT4G38000 50.000 120 40 3 26 143 42 143 1.62e-26 105
MS.gene01452.t1 AT3G50410 77.193 57 12 1 27 82 32 88 3.76e-26 105
MS.gene01452.t1 AT4G24060 73.684 57 15 0 23 79 51 107 3.81e-26 107
MS.gene01452.t1 AT2G46590 74.545 55 13 1 19 72 61 115 1.44e-24 102
MS.gene01452.t1 AT1G64620 66.129 62 16 1 18 74 37 98 1.52e-24 102
MS.gene01452.t1 AT1G47655 74.510 51 13 0 23 73 27 77 1.59e-24 99.4
MS.gene01452.t1 AT2G46590 74.545 55 13 1 19 72 73 127 1.82e-24 102
MS.gene01452.t1 AT5G62430 52.500 80 35 1 2 78 28 107 2.52e-23 98.2
MS.gene01452.t1 AT4G21040 62.687 67 25 0 27 93 27 93 3.96e-23 96.3
MS.gene01452.t1 AT3G47500 70.370 54 16 0 27 80 112 165 6.00e-23 99.4
MS.gene01452.t1 AT1G69570 58.209 67 28 0 12 78 119 185 1.15e-22 98.2
MS.gene01452.t1 AT5G39660 57.971 69 25 1 10 78 127 191 1.90e-22 98.2
MS.gene01452.t1 AT5G39660 57.971 69 25 1 10 78 127 191 1.90e-22 98.2
MS.gene01452.t1 AT5G39660 57.971 69 25 1 10 78 127 191 1.90e-22 98.2
MS.gene01452.t1 AT2G34140 57.746 71 27 1 11 78 41 111 3.78e-22 92.0
MS.gene01452.t1 AT1G29160 59.701 67 24 1 15 78 49 115 4.74e-22 92.0
MS.gene01452.t1 AT4G21080 66.667 57 18 1 27 83 27 82 9.43e-22 93.2
MS.gene01452.t1 AT1G26790 65.385 52 18 0 27 78 113 164 1.26e-20 92.0
MS.gene01452.t1 AT4G21030 48.611 72 37 0 22 93 18 89 2.91e-16 76.6

Find 92 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCCTCTGATCAATCTTTA+TGG 0.208599 2.2:-68760665 MS.gene01452:CDS
GGTAGCGGTGGTGTTGCTGC+TGG 0.285628 2.2:-68760528 MS.gene01452:CDS
CAACATTGAAACCTTGTAAA+AGG 0.337370 2.2:+68760910 None:intergenic
GGGTAGTGGATCTACTTCTT+TGG 0.352895 2.2:-68760505 MS.gene01452:CDS
GTTGGAGTTGGTGTTGGTCT+TGG 0.366325 2.2:-68760951 MS.gene01452:CDS
GTAGCGGTGGTGTTGCTGCT+GGG 0.368263 2.2:-68760527 MS.gene01452:CDS
ATTGGTGTTGGTGCTGGTGC+TGG 0.371549 2.2:-68760594 MS.gene01452:CDS
CTTCTTTGGCTCTGAATCAA+TGG 0.382630 2.2:-68760491 MS.gene01452:CDS
TGGTGTTGAGGATGAAGTTT+TGG 0.384562 2.2:+68761217 None:intergenic
TTCTTTCAACTAGGTGGTAA+TGG 0.386741 2.2:-68760831 MS.gene01452:CDS
TTCCCTAAGGAATTACTTGA+TGG 0.409259 2.2:+68761197 None:intergenic
CTGCTACTTATAATGAAAGT+TGG 0.411866 2.2:-68760719 MS.gene01452:CDS
ACACCATCAAGTAATTCCTT+AGG 0.417503 2.2:-68761200 MS.gene01452:CDS
CCACTATTGATGAAGCTTTG+AGG 0.422498 2.2:+68760696 None:intergenic
ATCAACACCACTTCAACTAA+TGG 0.426570 2.2:-68760636 MS.gene01452:CDS
AATCAATGGCATGATCTGTC+AGG 0.428092 2.2:-68760477 MS.gene01452:CDS
TGTTGCGGAGGTGGACGATT+CGG 0.432201 2.2:+68760855 None:intergenic
CCATCGCAGCCATGGTCAAC+TGG 0.443933 2.2:+68761135 None:intergenic
TATTGGTGGTGGTTGTCGTA+AGG 0.444825 2.2:-68761249 MS.gene01452:CDS
GCAAAACATGCAGAAGGTAC+TGG 0.452080 2.2:-68761301 MS.gene01452:CDS
GAAAGGATTGGTGTTGGTGC+TGG 0.452826 2.2:-68760600 MS.gene01452:CDS
GAATGATTAAATGCCGCAAA+AGG 0.453309 2.2:+68761032 None:intergenic
TGAAAGAATTGTTGCGGAGG+TGG 0.459091 2.2:+68760846 None:intergenic
GGTAGTCGTGGTGTTGGTGT+TGG 0.463926 2.2:-68760570 MS.gene01452:CDS
GTAGCACTAACCATGCTAGA+TGG 0.465127 2.2:+68760762 None:intergenic
GTTGGTGGTAGTCGTGGTGC+TGG 0.471209 2.2:-68760552 MS.gene01452:CDS
AGTCGTGGTGCTGGTGGTAG+CGG 0.472929 2.2:-68760543 MS.gene01452:CDS
CACCATCAAGTAATTCCTTA+GGG 0.476186 2.2:-68761199 MS.gene01452:CDS
TAGCGGTGGTGTTGCTGCTG+GGG 0.478385 2.2:-68760526 MS.gene01452:CDS
TTACGACAACCACCACCAAT+AGG 0.480617 2.2:+68761251 None:intergenic
ACATTGAGAAATGTTCCTAT+TGG 0.486939 2.2:-68761266 MS.gene01452:CDS
GTGGCAGTGTTGGCTGAGAC+AGG 0.487687 2.2:+68761168 None:intergenic
TTTGAACAGTCTCAGAATGT+TGG 0.491097 2.2:-68760969 MS.gene01452:CDS
CAGAATGTTGGAGTTGGTGT+TGG 0.491297 2.2:-68760957 MS.gene01452:CDS
TCCACCACCATTAGTTGAAG+TGG 0.494139 2.2:+68760629 None:intergenic
CCTCAAAGCTTCATCAATAG+TGG 0.497822 2.2:-68760696 MS.gene01452:CDS
CTAGGACACTTCTGAGGTTG+AGG 0.509691 2.2:+68761392 None:intergenic
AGTCGTGGTGTTGGTGTTGG+TGG 0.513731 2.2:-68760567 MS.gene01452:CDS
CTCAACCTCAGAAGTGTCCT+AGG 0.514646 2.2:-68761391 MS.gene01452:CDS
CCGCAACAATTCTTTCAACT+AGG 0.515108 2.2:-68760840 MS.gene01452:CDS
CCAGTTGACCATGGCTGCGA+TGG 0.534868 2.2:-68761135 MS.gene01452:CDS
TCGGTTGATTGTGGCAGTGT+TGG 0.542995 2.2:+68761158 None:intergenic
ACCACTTCAACTAATGGTGG+TGG 0.543681 2.2:-68760630 MS.gene01452:CDS
TTTGATTCCTCCATCTAGCA+TGG 0.549167 2.2:-68760772 MS.gene01452:CDS
ATTGGTGGTGGTTGTCGTAA+GGG 0.550492 2.2:-68761248 MS.gene01452:CDS
CGCAGCCATGGTCAACTGGT+CGG 0.551583 2.2:+68761139 None:intergenic
TTGAGAAATGTTCCTATTGG+TGG 0.552839 2.2:-68761263 MS.gene01452:CDS
AGAAATGTTCCTATTGGTGG+TGG 0.559899 2.2:-68761260 MS.gene01452:CDS
GCTGGTGGTAGTCGTGGTGT+TGG 0.563061 2.2:-68760576 MS.gene01452:CDS
AAGAACGTGGTGATGGTGGA+AGG 0.564755 2.2:-68761448 MS.gene01452:CDS
AGCGGTGGTGTTGCTGCTGG+GGG 0.569854 2.2:-68760525 MS.gene01452:CDS
GTTGTTGTAATAGCAGAACT+TGG 0.576616 2.2:+68761352 None:intergenic
GGTGTTGGTGCTGGTGCTGG+TGG 0.577339 2.2:-68760591 MS.gene01452:CDS
TGTGCTGAAGAGTGTGCTGA+TGG 0.579842 2.2:+68760996 None:intergenic
TGCAGAAGGTACTGGACTCA+AGG 0.580923 2.2:-68761293 MS.gene01452:CDS
CGTGGTGATGGTGGAAGGAG+AGG 0.581943 2.2:-68761443 MS.gene01452:CDS
GTCAACTGGTCGGTTGATTG+TGG 0.591951 2.2:+68761149 None:intergenic
ATTCAAACCTCGTGGTTCAG+AGG 0.592802 2.2:+68760794 None:intergenic
CGTGGTGCTGGTGGTAGCGG+TGG 0.594465 2.2:-68760540 MS.gene01452:CDS
GAGCAAGAACGTGGTGATGG+TGG 0.595958 2.2:-68761452 MS.gene01452:CDS
GCTGGTGCTGGTGGTAGTCG+TGG 0.610585 2.2:-68760582 MS.gene01452:CDS
CAACAATTCTTTCAACTAGG+TGG 0.614943 2.2:-68760837 MS.gene01452:CDS
ATCAATGGCATGATCTGTCA+GGG 0.615122 2.2:-68760476 MS.gene01452:CDS
GGAATTACTTGATGGTGTTG+AGG 0.615613 2.2:+68761205 None:intergenic
TCTTCTGCAAAACATGCAGA+AGG 0.615692 2.2:-68761307 MS.gene01452:CDS
GGTGGAAATTCTGAAAGGAT+TGG 0.619425 2.2:-68760612 MS.gene01452:CDS
AGAAGAAACGAGGCTGAGAG+AGG 0.619615 2.2:+68761324 None:intergenic
GGTGGTAGTCGTGGTGCTGG+TGG 0.619765 2.2:-68760549 MS.gene01452:CDS
ATGGAGCAAGAACGTGGTGA+TGG 0.620486 2.2:-68761455 MS.gene01452:CDS
CGTGGTTCAGAGGTAAACAT+TGG 0.628309 2.2:+68760804 None:intergenic
ATGGTGGTGGAAATTCTGAA+AGG 0.635909 2.2:-68760617 MS.gene01452:CDS
AATTCTGAAAGGATTGGTGT+TGG 0.637664 2.2:-68760606 MS.gene01452:CDS
GTTGGTGTTGGTGGTAGTCG+TGG 0.638430 2.2:-68760558 MS.gene01452:CDS
AACACCACTTCAACTAATGG+TGG 0.642679 2.2:-68760633 MS.gene01452:CDS
CAGTCTCAGAATGTTGGAGT+TGG 0.643457 2.2:-68760963 MS.gene01452:CDS
TTTCAACTAGGTGGTAATGG+AGG 0.647517 2.2:-68760828 MS.gene01452:CDS
CCTAGTTGAAAGAATTGTTG+CGG 0.647938 2.2:+68760840 None:intergenic
AGACAGGGAAGAATTCCCTA+AGG 0.650183 2.2:+68761184 None:intergenic
TCACACCTAGGACACTTCTG+AGG 0.655155 2.2:+68761386 None:intergenic
ATCAACCGACCAGTTGACCA+TGG 0.659300 2.2:-68761144 MS.gene01452:CDS
AGTTGAAAGAATTGTTGCGG+AGG 0.670800 2.2:+68760843 None:intergenic
CATCAACAAGATCACTGAGG+AGG 0.672156 2.2:+68760447 None:intergenic
GCACTAACCATGCTAGATGG+AGG 0.673922 2.2:+68760765 None:intergenic
GGAATCAAATTCAAACCTCG+TGG 0.678570 2.2:+68760786 None:intergenic
TGAGAGATGGAGCAAGAACG+TGG 0.685685 2.2:-68761461 None:intergenic
TGGCAGTGTTGGCTGAGACA+GGG 0.687589 2.2:+68761169 None:intergenic
ACTCCATAAAGATTGATCAG+AGG 0.693689 2.2:+68760662 None:intergenic
GTGTTGAATGAATCACACCT+AGG 0.703840 2.2:+68761374 None:intergenic
GCTCTTGTAAGCTCATCACA+AGG 0.707366 2.2:-68760879 MS.gene01452:CDS
AGAAGGTACTGGACTCAAGG+TGG 0.711298 2.2:-68761290 MS.gene01452:CDS
ATGTTTACCTCTGAACCACG+AGG 0.723992 2.2:-68760801 MS.gene01452:CDS
CAACATCAACAAGATCACTG+AGG 0.799835 2.2:+68760444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACATTGAGAAATGTTCCTAT+TGG - chr2.2:68760647-68760666 MS.gene01452:CDS 30.0%
CAACATTGAAACCTTGTAAA+AGG + chr2.2:68761006-68761025 None:intergenic 30.0%
! CTGCTACTTATAATGAAAGT+TGG - chr2.2:68761194-68761213 MS.gene01452:CDS 30.0%
! TCAAATTTGAGCCTTTTACA+AGG - chr2.2:68760992-68761011 MS.gene01452:CDS 30.0%
ACACCATCAAGTAATTCCTT+AGG - chr2.2:68760713-68760732 MS.gene01452:CDS 35.0%
ACTCCATAAAGATTGATCAG+AGG + chr2.2:68761254-68761273 None:intergenic 35.0%
ATCAACACCACTTCAACTAA+TGG - chr2.2:68761277-68761296 MS.gene01452:CDS 35.0%
CAACAATTCTTTCAACTAGG+TGG - chr2.2:68761076-68761095 MS.gene01452:CDS 35.0%
CACCATCAAGTAATTCCTTA+GGG - chr2.2:68760714-68760733 MS.gene01452:CDS 35.0%
CCTAGTTGAAAGAATTGTTG+CGG + chr2.2:68761076-68761095 None:intergenic 35.0%
GAATGATTAAATGCCGCAAA+AGG + chr2.2:68760884-68760903 None:intergenic 35.0%
GTTGTTGTAATAGCAGAACT+TGG + chr2.2:68760564-68760583 None:intergenic 35.0%
TTCCCTAAGGAATTACTTGA+TGG + chr2.2:68760719-68760738 None:intergenic 35.0%
TTCTTTCAACTAGGTGGTAA+TGG - chr2.2:68761082-68761101 MS.gene01452:CDS 35.0%
TTGAGAAATGTTCCTATTGG+TGG - chr2.2:68760650-68760669 MS.gene01452:CDS 35.0%
TTGATAAAAAGAAGCAGCAG+AGG + chr2.2:68760837-68760856 None:intergenic 35.0%
TTTGAACAGTCTCAGAATGT+TGG - chr2.2:68760944-68760963 MS.gene01452:CDS 35.0%
! AATTCTGAAAGGATTGGTGT+TGG - chr2.2:68761307-68761326 MS.gene01452:CDS 35.0%
!! TCTGCTGCTTCTTTTTATCA+AGG - chr2.2:68760836-68760855 MS.gene01452:CDS 35.0%
!!! TTTTATCAAGGAGGTGTTGT+TGG - chr2.2:68760848-68760867 MS.gene01452:CDS 35.0%
AACACCACTTCAACTAATGG+TGG - chr2.2:68761280-68761299 MS.gene01452:CDS 40.0%
AATCAATGGCATGATCTGTC+AGG - chr2.2:68761436-68761455 MS.gene01452:CDS 40.0%
AGAAATGTTCCTATTGGTGG+TGG - chr2.2:68760653-68760672 MS.gene01452:CDS 40.0%
AGTTGAAAGAATTGTTGCGG+AGG + chr2.2:68761073-68761092 None:intergenic 40.0%
ATCAATGGCATGATCTGTCA+GGG - chr2.2:68761437-68761456 MS.gene01452:CDS 40.0%
CCACTATTGATGAAGCTTTG+AGG + chr2.2:68761220-68761239 None:intergenic 40.0%
CCGCAACAATTCTTTCAACT+AGG - chr2.2:68761073-68761092 MS.gene01452:CDS 40.0%
CCTCAAAGCTTCATCAATAG+TGG - chr2.2:68761217-68761236 MS.gene01452:CDS 40.0%
GAGCCTCTGATCAATCTTTA+TGG - chr2.2:68761248-68761267 MS.gene01452:CDS 40.0%
GGAATCAAATTCAAACCTCG+TGG + chr2.2:68761130-68761149 None:intergenic 40.0%
GTGTTGAATGAATCACACCT+AGG + chr2.2:68760542-68760561 None:intergenic 40.0%
TCTTCTGCAAAACATGCAGA+AGG - chr2.2:68760606-68760625 MS.gene01452:CDS 40.0%
TTTGATTCCTCCATCTAGCA+TGG - chr2.2:68761141-68761160 MS.gene01452:CDS 40.0%
! CATGTTTTGCAGAAGAAACG+AGG + chr2.2:68760602-68760621 None:intergenic 40.0%
! GCTGCTTCTTTTTATCAAGG+AGG - chr2.2:68760839-68760858 MS.gene01452:CDS 40.0%
! GGTGGAAATTCTGAAAGGAT+TGG - chr2.2:68761301-68761320 MS.gene01452:CDS 40.0%
!! ATGGTGGTGGAAATTCTGAA+AGG - chr2.2:68761296-68761315 MS.gene01452:CDS 40.0%
!! CTTCTTTGGCTCTGAATCAA+TGG - chr2.2:68761422-68761441 MS.gene01452:CDS 40.0%
!! GGAATTACTTGATGGTGTTG+AGG + chr2.2:68760711-68760730 None:intergenic 40.0%
!! TGGTGTTGAGGATGAAGTTT+TGG + chr2.2:68760699-68760718 None:intergenic 40.0%
!! TTTCAACTAGGTGGTAATGG+AGG - chr2.2:68761085-68761104 MS.gene01452:CDS 40.0%
ACCACTTCAACTAATGGTGG+TGG - chr2.2:68761283-68761302 MS.gene01452:CDS 45.0%
AGACAGGGAAGAATTCCCTA+AGG + chr2.2:68760732-68760751 None:intergenic 45.0%
ATGTTTACCTCTGAACCACG+AGG - chr2.2:68761112-68761131 MS.gene01452:CDS 45.0%
ATTCAAACCTCGTGGTTCAG+AGG + chr2.2:68761122-68761141 None:intergenic 45.0%
CAGTCTCAGAATGTTGGAGT+TGG - chr2.2:68760950-68760969 MS.gene01452:CDS 45.0%
CGTGGTTCAGAGGTAAACAT+TGG + chr2.2:68761112-68761131 None:intergenic 45.0%
GCTCTTGTAAGCTCATCACA+AGG - chr2.2:68761034-68761053 MS.gene01452:CDS 45.0%
GTAGCACTAACCATGCTAGA+TGG + chr2.2:68761154-68761173 None:intergenic 45.0%
TCCACCACCATTAGTTGAAG+TGG + chr2.2:68761287-68761306 None:intergenic 45.0%
TGAAAGAATTGTTGCGGAGG+TGG + chr2.2:68761070-68761089 None:intergenic 45.0%
TTACGACAACCACCACCAAT+AGG + chr2.2:68760665-68760684 None:intergenic 45.0%
! GCAAAACATGCAGAAGGTAC+TGG - chr2.2:68760612-68760631 MS.gene01452:CDS 45.0%
! TGGTTACATGTCGCCTTTTG+CGG - chr2.2:68760868-68760887 MS.gene01452:CDS 45.0%
!! ATTGGTGGTGGTTGTCGTAA+GGG - chr2.2:68760665-68760684 MS.gene01452:CDS 45.0%
!! CAGAATGTTGGAGTTGGTGT+TGG - chr2.2:68760956-68760975 MS.gene01452:CDS 45.0%
!! GGGTAGTGGATCTACTTCTT+TGG - chr2.2:68761408-68761427 MS.gene01452:CDS 45.0%
!! TATTGGTGGTGGTTGTCGTA+AGG - chr2.2:68760664-68760683 MS.gene01452:CDS 45.0%
AGAAGAAACGAGGCTGAGAG+AGG + chr2.2:68760592-68760611 None:intergenic 50.0%
ATCAACCGACCAGTTGACCA+TGG - chr2.2:68760769-68760788 MS.gene01452:CDS 50.0%
ATGGAGCAAGAACGTGGTGA+TGG - chr2.2:68760458-68760477 MS.gene01452:CDS 50.0%
GCACTAACCATGCTAGATGG+AGG + chr2.2:68761151-68761170 None:intergenic 50.0%
GTCAACTGGTCGGTTGATTG+TGG + chr2.2:68760767-68760786 None:intergenic 50.0%
TCACACCTAGGACACTTCTG+AGG + chr2.2:68760530-68760549 None:intergenic 50.0%
TCGGTTGATTGTGGCAGTGT+TGG + chr2.2:68760758-68760777 None:intergenic 50.0%
! TGTGCTGAAGAGTGTGCTGA+TGG + chr2.2:68760920-68760939 None:intergenic 50.0%
!! AAGAACGTGGTGATGGTGGA+AGG - chr2.2:68760465-68760484 MS.gene01452:CDS 50.0%
!! AGAAGGTACTGGACTCAAGG+TGG - chr2.2:68760623-68760642 MS.gene01452:CDS 50.0%
!! CTAGGACACTTCTGAGGTTG+AGG + chr2.2:68760524-68760543 None:intergenic 50.0%
!! CTCAACCTCAGAAGTGTCCT+AGG - chr2.2:68760522-68760541 MS.gene01452:CDS 50.0%
!! GAAAGGATTGGTGTTGGTGC+TGG - chr2.2:68761313-68761332 MS.gene01452:CDS 50.0%
!! GTTGGAGTTGGTGTTGGTCT+TGG - chr2.2:68760962-68760981 MS.gene01452:CDS 50.0%
!! TGCAGAAGGTACTGGACTCA+AGG - chr2.2:68760620-68760639 MS.gene01452:CDS 50.0%
!!! GTTTTTAGCCATCGCAGCCA+TGG + chr2.2:68760789-68760808 None:intergenic 50.0%
TGTTGCGGAGGTGGACGATT+CGG + chr2.2:68761061-68761080 None:intergenic 55.0%
!! AGTCGTGGTGTTGGTGTTGG+TGG - chr2.2:68761346-68761365 MS.gene01452:CDS 55.0%
!! ATTGGTGTTGGTGCTGGTGC+TGG - chr2.2:68761319-68761338 MS.gene01452:CDS 55.0%
!! GAGCAAGAACGTGGTGATGG+TGG - chr2.2:68760461-68760480 MS.gene01452:CDS 55.0%
!! GGTAGTCGTGGTGTTGGTGT+TGG - chr2.2:68761343-68761362 MS.gene01452:CDS 55.0%
!! GTTGGTGTTGGTGGTAGTCG+TGG - chr2.2:68761355-68761374 MS.gene01452:CDS 55.0%
!! TGGCAGTGTTGGCTGAGACA+GGG + chr2.2:68760747-68760766 None:intergenic 55.0%
CCAGTTGACCATGGCTGCGA+TGG - chr2.2:68760778-68760797 MS.gene01452:CDS 60.0%
CCATCGCAGCCATGGTCAAC+TGG + chr2.2:68760781-68760800 None:intergenic 60.0%
CGCAGCCATGGTCAACTGGT+CGG + chr2.2:68760777-68760796 None:intergenic 60.0%
GCTGGTGGTAGTCGTGGTGT+TGG - chr2.2:68761337-68761356 MS.gene01452:CDS 60.0%
!! AGTCGTGGTGCTGGTGGTAG+CGG - chr2.2:68761370-68761389 MS.gene01452:CDS 60.0%
!! CGTGGTGATGGTGGAAGGAG+AGG - chr2.2:68760470-68760489 MS.gene01452:CDS 60.0%
!! GTAGCGGTGGTGTTGCTGCT+GGG - chr2.2:68761386-68761405 MS.gene01452:CDS 60.0%
!! GTGGCAGTGTTGGCTGAGAC+AGG + chr2.2:68760748-68760767 None:intergenic 60.0%
!! GTTGGTGGTAGTCGTGGTGC+TGG - chr2.2:68761361-68761380 MS.gene01452:CDS 60.0%
!! TAGCGGTGGTGTTGCTGCTG+GGG - chr2.2:68761387-68761406 MS.gene01452:CDS 60.0%
! GGTGGTAGTCGTGGTGCTGG+TGG - chr2.2:68761364-68761383 MS.gene01452:CDS 65.0%
!! AGCGGTGGTGTTGCTGCTGG+GGG - chr2.2:68761388-68761407 MS.gene01452:CDS 65.0%
!! GCTGGTGCTGGTGGTAGTCG+TGG - chr2.2:68761331-68761350 MS.gene01452:CDS 65.0%
!! GGTAGCGGTGGTGTTGCTGC+TGG - chr2.2:68761385-68761404 MS.gene01452:CDS 65.0%
!! GGTGTTGCTGCTGGGGGTAG+TGG - chr2.2:68761394-68761413 MS.gene01452:CDS 65.0%
!! GGTGTTGGTGCTGGTGCTGG+TGG - chr2.2:68761322-68761341 MS.gene01452:CDS 65.0%
!! CGTGGTGCTGGTGGTAGCGG+TGG - chr2.2:68761373-68761392 MS.gene01452:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 68760458 68761477 68760458 ID=MS.gene01452
chr2.2 mRNA 68760458 68761477 68760458 ID=MS.gene01452.t1;Parent=MS.gene01452
chr2.2 exon 68760458 68761477 68760458 ID=MS.gene01452.t1.exon1;Parent=MS.gene01452.t1
chr2.2 CDS 68760458 68761477 68760458 ID=cds.MS.gene01452.t1;Parent=MS.gene01452.t1
Gene Sequence

>MS.gene01452

ATGGAGCAAGAACGTGGTGATGGTGGAAGGAGAGGTGAGATGAGTAAGCATGTTCAAGCTCATCCTCAACCTCAGAAGTGTCCTAGGTGTGATTCATTCAACACCAAGTTCTGCTATTACAACAACTATAGCCTCTCTCAGCCTCGTTTCTTCTGCAAAACATGCAGAAGGTACTGGACTCAAGGTGGAACATTGAGAAATGTTCCTATTGGTGGTGGTTGTCGTAAGGGAAAGAAATCCAAAACTTCATCCTCAACACCATCAAGTAATTCCTTAGGGAATTCTTCCCTGTCTCAGCCAACACTGCCACAATCAACCGACCAGTTGACCATGGCTGCGATGGCTAAAAACTCTTCTTCTGTTTTTGCTTCATTTTCCTCTGCTGCTTCTTTTTATCAAGGAGGTGTTGTTGGTTACATGTCGCCTTTTGCGGCATTTAATCATTCGTTGAATTCGAATCCATCAGCACACTCTTCAGCACACACTTTTGAACAGTCTCAGAATGTTGGAGTTGGTGTTGGTCTTGGTTCTTCTTCAAATTTGAGCCTTTTACAAGGTTTCAATGTTGCTGCTGCTGCTCTTGTAAGCTCATCACAAGGACCGAATCGTCCACCTCCGCAACAATTCTTTCAACTAGGTGGTAATGGAGGACCAATGTTTACCTCTGAACCACGAGGTTTGAATTTGATTCCTCCATCTAGCATGGTTAGTGCTACTACTTCTGTTGCTACTGATTCTGCTACTTATAATGAAAGTTGGCCTCAAAGCTTCATCAATAGTGGTAACAACAGAGCCTCTGATCAATCTTTATGGAGTACTATCAACACCACTTCAACTAATGGTGGTGGAAATTCTGAAAGGATTGGTGTTGGTGCTGGTGCTGGTGGTAGTCGTGGTGTTGGTGTTGGTGGTAGTCGTGGTGCTGGTGGTAGCGGTGGTGTTGCTGCTGGGGGTAGTGGATCTACTTCTTTGGCTCTGAATCAATGGCATGATCTGTCAGGGTTCAATCCTCCTCAGTGA
Protein sequence

>MS.gene01452.t1

MEQERGDGGRRGEMSKHVQAHPQPQKCPRCDSFNTKFCYYNNYSLSQPRFFCKTCRRYWTQGGTLRNVPIGGGCRKGKKSKTSSSTPSSNSLGNSSLSQPTLPQSTDQLTMAAMAKNSSSVFASFSSAASFYQGGVVGYMSPFAAFNHSLNSNPSAHSSAHTFEQSQNVGVGVGLGSSSNLSLLQGFNVAAAALVSSSQGPNRPPPQQFFQLGGNGGPMFTSEPRGLNLIPPSSMVSATTSVATDSATYNESWPQSFINSGNNRASDQSLWSTINTTSTNGGGNSERIGVGAGAGGSRGVGVGGSRGAGGSGGVAAGGSGSTSLALNQWHDLSGFNPPQ