AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014526


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014526.t1 MTR_3g111780 94.718 284 14 1 1 284 1 283 0.0 501
MS.gene014526.t1 MTR_8g103520 50.000 72 36 0 193 264 218 289 5.16e-20 87.8
MS.gene014526.t1 MTR_0907s0020 52.941 68 32 0 197 264 100 167 8.28e-20 85.1
MS.gene014526.t1 MTR_5g075210 58.462 65 27 0 199 263 280 344 6.43e-19 85.9
MS.gene014526.t1 MTR_7g105790 53.623 69 32 0 202 270 113 181 5.47e-18 80.5
MS.gene014526.t1 MTR_7g072875 32.468 154 80 2 129 260 129 280 5.60e-18 82.4
MS.gene014526.t1 MTR_4g052040 53.846 65 30 0 200 264 100 164 1.82e-17 78.6
MS.gene014526.t1 MTR_5g079710 50.725 69 34 0 196 264 88 156 9.95e-17 78.2
MS.gene014526.t1 MTR_7g072630 26.829 287 155 8 27 260 58 342 1.09e-16 79.3
MS.gene014526.t1 MTR_8g087530 47.692 65 34 0 199 263 258 322 6.48e-16 77.0
MS.gene014526.t1 MTR_2g018030 46.774 62 33 0 199 260 277 338 9.80e-16 76.6
MS.gene014526.t1 MTR_4g028930 50.820 61 30 0 200 260 315 375 6.36e-15 74.3
MS.gene014526.t1 MTR_4g052060 51.667 60 29 0 205 264 99 158 1.91e-13 67.8
MS.gene014526.t1 MTR_1g111640 45.161 62 34 0 198 259 136 197 3.67e-11 62.4
MS.gene014526.t1 MTR_4g132740 37.500 88 44 1 178 265 71 147 5.82e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014526.t1 AT5G19650 62.121 66 25 0 199 264 154 219 7.03e-23 94.4
MS.gene014526.t1 AT2G18500 61.538 65 25 0 199 263 226 290 2.81e-20 89.0
MS.gene014526.t1 AT3G52525 55.385 65 29 0 197 261 64 128 2.10e-18 80.5
MS.gene014526.t1 AT2G30400 52.308 65 31 0 199 263 252 316 3.14e-18 83.6
MS.gene014526.t1 AT5G58360 53.846 65 30 0 199 263 224 288 3.68e-17 80.1
MS.gene014526.t1 AT2G36026 55.000 60 27 0 205 264 92 151 4.75e-17 77.8
MS.gene014526.t1 AT1G06920 33.010 103 64 1 167 264 206 308 3.53e-16 77.8
MS.gene014526.t1 AT5G01840 48.529 68 35 0 203 270 202 269 3.71e-15 73.9
MS.gene014526.t1 AT5G22240 44.118 68 38 0 196 263 93 160 4.25e-13 67.0
MS.gene014526.t1 AT4G18830 45.902 61 33 0 199 259 282 342 7.42e-12 65.5

Find 67 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGCAGGGCCTGGATTCC+CGG 0.272464 3.1:+89251814 None:intergenic
CTCAAATTATAGTGATAAAA+AGG 0.274257 3.1:-89251510 MS.gene014526:CDS
GGTCCATTCTTCTAGTACTA+TGG 0.311039 3.1:-89251555 MS.gene014526:CDS
TGCGAGCTTTCATGGAGATT+TGG 0.332540 3.1:-89251133 MS.gene014526:CDS
AGTAATTGTGCGAGCTTTCA+TGG 0.344864 3.1:-89251141 MS.gene014526:CDS
GACACGTTGCATTGGTGTTC+CGG 0.349518 3.1:+89251725 None:intergenic
CACCGGAGAAAGACGGTATA+TGG 0.359937 3.1:+89251796 None:intergenic
TCCTCTTTCCCCGGGAATCC+AGG 0.367021 3.1:-89251824 MS.gene014526:CDS
TTTGGCAACAAATGTTCGTT+TGG 0.381425 3.1:-89251115 MS.gene014526:CDS
GGCGACGAAGCAGGAAGGTT+TGG 0.382600 3.1:+89251746 None:intergenic
TTATCACTATAATTTGAGAT+AGG 0.385663 3.1:+89251515 None:intergenic
ATACCCTATGTCATATGCTT+TGG 0.387292 3.1:+89251769 None:intergenic
GGCAAAAGAAAGCTTTGCTT+TGG 0.397965 3.1:-89251303 MS.gene014526:CDS
AGTGAGGTTTCAAAGTATTA+CGG 0.405418 3.1:-89251426 MS.gene014526:CDS
ATTTAACTAGCTCGAATAGA+AGG 0.424510 3.1:-89251478 MS.gene014526:CDS
AATGACCTTTCCTCTTTCCC+CGG 0.427656 3.1:-89251833 MS.gene014526:CDS
ACTTGACATAACCTTCGTTT+CGG 0.428668 3.1:+89251340 None:intergenic
GATGTCCGAGGCCAAGGACT+TGG 0.437700 3.1:-89251213 MS.gene014526:CDS
ATGGCAGGGCCTGGATTCCC+GGG 0.439698 3.1:+89251815 None:intergenic
GTTAGAGATGATAGAAGAGA+TGG 0.441612 3.1:-89251237 MS.gene014526:CDS
TTCTTGCTTAGCAAGATTCT+CGG 0.449560 3.1:-89251588 MS.gene014526:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.1:-89251333 MS.gene014526:CDS
GAAGGTTATGTCAAGTAGAA+TGG 0.463702 3.1:-89251381 MS.gene014526:CDS
CTATGGATAGTACTAAAGTT+AGG 0.470027 3.1:-89251538 MS.gene014526:CDS
TGAAGAAGGTTGAGAGAGTG+AGG 0.472397 3.1:-89251442 MS.gene014526:CDS
CAATAGTTGCTCCAAGTCCT+TGG 0.478606 3.1:+89251202 None:intergenic
TCAAACAAATGTTCAAACTA+AGG 0.481359 3.1:-89251069 MS.gene014526:CDS
GGGGAAAGAGGAAAGGTCAT+TGG 0.484366 3.1:+89251835 None:intergenic
AGACGGTATATGGCAGGGCC+TGG 0.484707 3.1:+89251806 None:intergenic
ACTAGAAGAATGGACCTTCT+CGG 0.488884 3.1:+89251562 None:intergenic
GCCTGGATTCCCGGGGAAAG+AGG 0.494617 3.1:+89251823 None:intergenic
TATCCATAGTACTAGAAGAA+TGG 0.495158 3.1:+89251552 None:intergenic
AGAAACTCACTTCTTCTTGA+GGG 0.501729 3.1:+89251650 None:intergenic
TGGTGTTCCGGCGACGAAGC+AGG 0.502249 3.1:+89251737 None:intergenic
GACGATAAGTCGTGTGAAGA+AGG 0.503045 3.1:-89251456 MS.gene014526:CDS
GATGGAGATGTCCGAGGCCA+AGG 0.503048 3.1:-89251219 MS.gene014526:CDS
TGCCATATACCGTCTTTCTC+CGG 0.503136 3.1:-89251798 MS.gene014526:CDS
GAGCGACACATTTGAAAAGA+AGG 0.503682 3.1:+89251857 None:intergenic
AAACCTTCCTGCTTCGTCGC+CGG 0.514759 3.1:-89251744 MS.gene014526:CDS
TTGCTCCAAGTCCTTGGCCT+CGG 0.515968 3.1:+89251208 None:intergenic
AGCGACACATTTGAAAAGAA+GGG 0.522344 3.1:+89251858 None:intergenic
ATGACCTTTCCTCTTTCCCC+GGG 0.522963 3.1:-89251832 MS.gene014526:CDS
GGAGAAAGACGGTATATGGC+AGG 0.531009 3.1:+89251800 None:intergenic
TAGAAACTCACTTCTTCTTG+AGG 0.531127 3.1:+89251649 None:intergenic
TCATATGCTTTGGAGTTCAC+CGG 0.541099 3.1:+89251779 None:intergenic
CTAAATTCTAGAGATTATCA+TGG 0.544872 3.1:-89251164 MS.gene014526:CDS
GTCAAGTAGAATGGAGGAGA+AGG 0.546067 3.1:-89251324 MS.gene014526:CDS
GATTTCTTGAAGTCTTCGTA+AGG 0.562850 3.1:+89251263 None:intergenic
AATTGATAAAATCAATGAAG+AGG 0.573253 3.1:-89251627 MS.gene014526:CDS
AAGATTCTCGGAAGCCGAGA+AGG 0.578226 3.1:-89251576 MS.gene014526:CDS
AACTCCAAAGCATATGACAT+AGG 0.582440 3.1:-89251773 MS.gene014526:CDS
TCTTTGATGACACGTTGCAT+TGG 0.597146 3.1:+89251717 None:intergenic
TCTTCTTGAGGGAACGTCGG+AGG 0.601171 3.1:+89251661 None:intergenic
ACTCCAAAGCATATGACATA+GGG 0.601832 3.1:-89251772 MS.gene014526:CDS
TCGAAGAGGAACCGAAACGA+AGG 0.602284 3.1:-89251351 MS.gene014526:CDS
ATTAAGAAAGTGAAATCCAA+AGG 0.608408 3.1:-89251695 MS.gene014526:CDS
TGGAGTTCACCGGAGAAAGA+CGG 0.608559 3.1:+89251789 None:intergenic
GTTCCGGCGACGAAGCAGGA+AGG 0.630275 3.1:+89251741 None:intergenic
GATTCCCGGGGAAAGAGGAA+AGG 0.637825 3.1:+89251828 None:intergenic
GAGAAAGACGGTATATGGCA+GGG 0.639308 3.1:+89251801 None:intergenic
ACTTCTTCTTGAGGGAACGT+CGG 0.649804 3.1:+89251658 None:intergenic
AGTATTACGGAGAATCAAAG+AGG 0.650942 3.1:-89251413 MS.gene014526:CDS
TGGCAGGGCCTGGATTCCCG+GGG 0.653477 3.1:+89251816 None:intergenic
GGTTATGTCAAGTAGAATGG+AGG 0.656874 3.1:-89251330 MS.gene014526:CDS
AGAATGGAAGAGAATCGAAG+AGG 0.657497 3.1:-89251365 MS.gene014526:CDS
TCAAAGAGGAAAAGAAACGA+AGG 0.672215 3.1:-89251399 MS.gene014526:CDS
AGAAGAGATGGAGATGTCCG+AGG 0.675634 3.1:-89251225 MS.gene014526:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATAAAATCAATGAAG+AGG - chr3.1:89251318-89251337 MS.gene014526:CDS 20.0%
!! CTCAAATTATAGTGATAAAA+AGG - chr3.1:89251435-89251454 MS.gene014526:CDS 20.0%
!!! TTATCACTATAATTTGAGAT+AGG + chr3.1:89251433-89251452 None:intergenic 20.0%
! ATTAAGAAAGTGAAATCCAA+AGG - chr3.1:89251250-89251269 MS.gene014526:CDS 25.0%
! TCAAACAAATGTTCAAACTA+AGG - chr3.1:89251876-89251895 MS.gene014526:CDS 25.0%
!! CTAAATTCTAGAGATTATCA+TGG - chr3.1:89251781-89251800 MS.gene014526:CDS 25.0%
!!! TGAAGATTTTTTACTGACTT+AGG + chr3.1:89251859-89251878 None:intergenic 25.0%
ATTTAACTAGCTCGAATAGA+AGG - chr3.1:89251467-89251486 MS.gene014526:CDS 30.0%
CTATGGATAGTACTAAAGTT+AGG - chr3.1:89251407-89251426 MS.gene014526:CDS 30.0%
TATCCATAGTACTAGAAGAA+TGG + chr3.1:89251396-89251415 None:intergenic 30.0%
! AGTGAGGTTTCAAAGTATTA+CGG - chr3.1:89251519-89251538 MS.gene014526:CDS 30.0%
AACTCCAAAGCATATGACAT+AGG - chr3.1:89251172-89251191 MS.gene014526:CDS 35.0%
ACTCCAAAGCATATGACATA+GGG - chr3.1:89251173-89251192 MS.gene014526:CDS 35.0%
ACTTGACATAACCTTCGTTT+CGG + chr3.1:89251560-89251579 None:intergenic 35.0%
AGAAACTCACTTCTTCTTGA+GGG + chr3.1:89251298-89251317 None:intergenic 35.0%
AGCGACACATTTGAAAAGAA+GGG + chr3.1:89251090-89251109 None:intergenic 35.0%
AGTATTACGGAGAATCAAAG+AGG - chr3.1:89251532-89251551 MS.gene014526:CDS 35.0%
ATACCCTATGTCATATGCTT+TGG + chr3.1:89251179-89251198 None:intergenic 35.0%
GAAGGTTATGTCAAGTAGAA+TGG - chr3.1:89251564-89251583 MS.gene014526:CDS 35.0%
GATTTCTTGAAGTCTTCGTA+AGG + chr3.1:89251685-89251704 None:intergenic 35.0%
GTTAGAGATGATAGAAGAGA+TGG - chr3.1:89251708-89251727 MS.gene014526:CDS 35.0%
TAGAAACTCACTTCTTCTTG+AGG + chr3.1:89251299-89251318 None:intergenic 35.0%
TCAAAGAGGAAAAGAAACGA+AGG - chr3.1:89251546-89251565 MS.gene014526:CDS 35.0%
TGCTTTGGTGAAAAAGTCAA+AGG - chr3.1:89251657-89251676 MS.gene014526:CDS 35.0%
TTCTTGCTTAGCAAGATTCT+CGG - chr3.1:89251357-89251376 MS.gene014526:CDS 35.0%
TTTGGCAACAAATGTTCGTT+TGG - chr3.1:89251830-89251849 MS.gene014526:CDS 35.0%
!!! GCAACTATTGCAGTGTTTTT+TGG - chr3.1:89251756-89251775 MS.gene014526:CDS 35.0%
ACTAGAAGAATGGACCTTCT+CGG + chr3.1:89251386-89251405 None:intergenic 40.0%
AGAATGGAAGAGAATCGAAG+AGG - chr3.1:89251580-89251599 MS.gene014526:CDS 40.0%
AGTAATTGTGCGAGCTTTCA+TGG - chr3.1:89251804-89251823 MS.gene014526:CDS 40.0%
GAGCGACACATTTGAAAAGA+AGG + chr3.1:89251091-89251110 None:intergenic 40.0%
GGCAAAAGAAAGCTTTGCTT+TGG - chr3.1:89251642-89251661 MS.gene014526:CDS 40.0%
GGTCCATTCTTCTAGTACTA+TGG - chr3.1:89251390-89251409 MS.gene014526:CDS 40.0%
GGTTATGTCAAGTAGAATGG+AGG - chr3.1:89251567-89251586 MS.gene014526:CDS 40.0%
! TCTTTGATGACACGTTGCAT+TGG + chr3.1:89251231-89251250 None:intergenic 40.0%
!! TCATATGCTTTGGAGTTCAC+CGG + chr3.1:89251169-89251188 None:intergenic 40.0%
AATGACCTTTCCTCTTTCCC+CGG - chr3.1:89251112-89251131 MS.gene014526:CDS 45.0%
ACTTCTTCTTGAGGGAACGT+CGG + chr3.1:89251290-89251309 None:intergenic 45.0%
CAATAGTTGCTCCAAGTCCT+TGG + chr3.1:89251746-89251765 None:intergenic 45.0%
GACGATAAGTCGTGTGAAGA+AGG - chr3.1:89251489-89251508 MS.gene014526:CDS 45.0%
GAGAAAGACGGTATATGGCA+GGG + chr3.1:89251147-89251166 None:intergenic 45.0%
GTCAAGTAGAATGGAGGAGA+AGG - chr3.1:89251621-89251640 MS.gene014526:CDS 45.0%
TGAAGAAGGTTGAGAGAGTG+AGG - chr3.1:89251503-89251522 MS.gene014526:CDS 45.0%
TGCCATATACCGTCTTTCTC+CGG - chr3.1:89251147-89251166 MS.gene014526:CDS 45.0%
TGCGAGCTTTCATGGAGATT+TGG - chr3.1:89251812-89251831 MS.gene014526:CDS 45.0%
AAGATTCTCGGAAGCCGAGA+AGG - chr3.1:89251369-89251388 MS.gene014526:CDS 50.0%
AGAAGAGATGGAGATGTCCG+AGG - chr3.1:89251720-89251739 MS.gene014526:CDS 50.0%
ATGACCTTTCCTCTTTCCCC+GGG - chr3.1:89251113-89251132 MS.gene014526:CDS 50.0%
CACCGGAGAAAGACGGTATA+TGG + chr3.1:89251152-89251171 None:intergenic 50.0%
GGAGAAAGACGGTATATGGC+AGG + chr3.1:89251148-89251167 None:intergenic 50.0%
GGGGAAAGAGGAAAGGTCAT+TGG + chr3.1:89251113-89251132 None:intergenic 50.0%
TCGAAGAGGAACCGAAACGA+AGG - chr3.1:89251594-89251613 MS.gene014526:CDS 50.0%
TGGAGTTCACCGGAGAAAGA+CGG + chr3.1:89251159-89251178 None:intergenic 50.0%
! GACACGTTGCATTGGTGTTC+CGG + chr3.1:89251223-89251242 None:intergenic 50.0%
AAACCTTCCTGCTTCGTCGC+CGG - chr3.1:89251201-89251220 MS.gene014526:CDS 55.0%
GATTCCCGGGGAAAGAGGAA+AGG + chr3.1:89251120-89251139 None:intergenic 55.0%
TCTTCTTGAGGGAACGTCGG+AGG + chr3.1:89251287-89251306 None:intergenic 55.0%
TTGCTCCAAGTCCTTGGCCT+CGG + chr3.1:89251740-89251759 None:intergenic 55.0%
!!! GAACGTCGGAGGTGCTTTTG+CGG + chr3.1:89251276-89251295 None:intergenic 55.0%
AGACGGTATATGGCAGGGCC+TGG + chr3.1:89251142-89251161 None:intergenic 60.0%
GATGGAGATGTCCGAGGCCA+AGG - chr3.1:89251726-89251745 MS.gene014526:CDS 60.0%
GATGTCCGAGGCCAAGGACT+TGG - chr3.1:89251732-89251751 MS.gene014526:CDS 60.0%
GGCGACGAAGCAGGAAGGTT+TGG + chr3.1:89251202-89251221 None:intergenic 60.0%
TATGGCAGGGCCTGGATTCC+CGG + chr3.1:89251134-89251153 None:intergenic 60.0%
TCCTCTTTCCCCGGGAATCC+AGG - chr3.1:89251121-89251140 MS.gene014526:CDS 60.0%
!!! GGAGGTGCTTTTGCGGCCTT+TGG + chr3.1:89251269-89251288 None:intergenic 60.0%
ATGGCAGGGCCTGGATTCCC+GGG + chr3.1:89251133-89251152 None:intergenic 65.0%
GCCTGGATTCCCGGGGAAAG+AGG + chr3.1:89251125-89251144 None:intergenic 65.0%
GTTCCGGCGACGAAGCAGGA+AGG + chr3.1:89251207-89251226 None:intergenic 65.0%
TGGTGTTCCGGCGACGAAGC+AGG + chr3.1:89251211-89251230 None:intergenic 65.0%
TGGCAGGGCCTGGATTCCCG+GGG + chr3.1:89251132-89251151 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 89251055 89251912 89251055 ID=MS.gene014526
chr3.1 mRNA 89251055 89251912 89251055 ID=MS.gene014526.t1;Parent=MS.gene014526
chr3.1 exon 89251055 89251912 89251055 ID=MS.gene014526.t1.exon1;Parent=MS.gene014526.t1
chr3.1 CDS 89251055 89251912 89251055 ID=cds.MS.gene014526.t1;Parent=MS.gene014526.t1
Gene Sequence

>MS.gene014526

ATGTCTAAACGCTTTAAGATGAAATTTACAATCCCTTCTTTTCAAATGTGTCGCTCCAATGACCTTTCCTCTTTCCCCGGGAATCCAGGCCCTGCCATATACCGTCTTTCTCCGGTGAACTCCAAAGCATATGACATAGGGTATCCAAACCTTCCTGCTTCGTCGCCGGAACACCAATGCAACGTGTCATCAAAGATTAAGAAAGTGAAATCCAAAGGCCGCAAAAGCACCTCCGACGTTCCCTCAAGAAGAAGTGAGTTTCTAATTGATAAAATCAATGAAGAGGAAAGTGAATCATTGATTTCTTGCTTAGCAAGATTCTCGGAAGCCGAGAAGGTCCATTCTTCTAGTACTATGGATAGTACTAAAGTTAGGCCTATCTCAAATTATAGTGATAAAAAGGAGAATAAAGATTTAACTAGCTCGAATAGAAGGACGATAAGTCGTGTGAAGAAGGTTGAGAGAGTGAGGTTTCAAAGTATTACGGAGAATCAAAGAGGAAAAGAAACGAAGGTTATGTCAAGTAGAATGGAAGAGAATCGAAGAGGAACCGAAACGAAGGTTATGTCAAGTAGAATGGAGGAGAAGGCAAAAGAAAGCTTTGCTTTGGTGAAAAAGTCAAAGGATCCTTACGAAGACTTCAAGAAATCAATGTTAGAGATGATAGAAGAGATGGAGATGTCCGAGGCCAAGGACTTGGAGCAACTATTGCAGTGTTTTTTGGCTCTAAATTCTAGAGATTATCATGGAGTAATTGTGCGAGCTTTCATGGAGATTTGGCAACAAATGTTCGTTTGGAATCCTAAGTCAGTAAAAAATCTTCAAACAAATGTTCAAACTAAGGATGTAGAAAATTAG

Protein sequence

>MS.gene014526.t1

MSKRFKMKFTIPSFQMCRSNDLSSFPGNPGPAIYRLSPVNSKAYDIGYPNLPASSPEHQCNVSSKIKKVKSKGRKSTSDVPSRRSEFLIDKINEEESESLISCLARFSEAEKVHSSSTMDSTKVRPISNYSDKKENKDLTSSNRRTISRVKKVERVRFQSITENQRGKETKVMSSRMEENRRGTETKVMSSRMEEKAKESFALVKKSKDPYEDFKKSMLEMIEEMEMSEAKDLEQLLQCFLALNSRDYHGVIVRAFMEIWQQMFVWNPKSVKNLQTNVQTKDVEN