Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014532.t1 | XP_024634116.1 | 94.4 | 124 | 7 | 0 | 1 | 124 | 1 | 124 | 2.10E-57 | 231.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014532.t1 | Q9SCQ6 | 85.9 | 99 | 13 | 1 | 26 | 124 | 38 | 135 | 2.0e-48 | 193.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014532.t1 | A0A396IZ01 | 94.4 | 124 | 7 | 0 | 1 | 124 | 1 | 124 | 1.5e-57 | 231.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014532.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014532.t1 | MTR_2g099990 | 84.762 | 105 | 15 | 1 | 20 | 124 | 44 | 147 | 1.67e-63 | 200 |
| MS.gene014532.t1 | MTR_4g059870 | 79.439 | 107 | 22 | 0 | 18 | 124 | 34 | 140 | 1.60e-62 | 198 |
| MS.gene014532.t1 | MTR_1g016010 | 76.786 | 112 | 26 | 0 | 13 | 124 | 32 | 143 | 9.13e-62 | 195 |
| MS.gene014532.t1 | MTR_8g017210 | 82.569 | 109 | 18 | 1 | 16 | 124 | 56 | 163 | 1.62e-61 | 196 |
| MS.gene014532.t1 | MTR_1g094115 | 80.952 | 105 | 19 | 1 | 20 | 124 | 45 | 148 | 1.10e-59 | 192 |
| MS.gene014532.t1 | MTR_2g093960 | 78.641 | 103 | 22 | 0 | 22 | 124 | 32 | 134 | 3.82e-59 | 187 |
| MS.gene014532.t1 | MTR_1g112270 | 79.000 | 100 | 20 | 1 | 25 | 124 | 47 | 145 | 1.69e-55 | 181 |
| MS.gene014532.t1 | MTR_1g112270 | 71.171 | 111 | 20 | 2 | 25 | 124 | 47 | 156 | 5.96e-52 | 172 |
| MS.gene014532.t1 | MTR_2g090745 | 77.660 | 94 | 20 | 1 | 31 | 124 | 16 | 108 | 1.48e-50 | 166 |
| MS.gene014532.t1 | MTR_2g099990 | 84.810 | 79 | 11 | 1 | 46 | 124 | 1 | 78 | 5.39e-47 | 155 |
| MS.gene014532.t1 | MTR_2g017720 | 73.469 | 98 | 25 | 1 | 27 | 124 | 318 | 414 | 1.06e-46 | 155 |
| MS.gene014532.t1 | MTR_1g094115 | 64.762 | 105 | 19 | 2 | 20 | 124 | 45 | 131 | 8.97e-43 | 147 |
| MS.gene014532.t1 | MTR_3g102000 | 59.551 | 89 | 28 | 3 | 43 | 124 | 27 | 114 | 2.91e-30 | 108 |
| MS.gene014532.t1 | MTR_5g030560 | 77.778 | 45 | 10 | 0 | 32 | 76 | 16 | 60 | 4.44e-21 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014532.t1 | AT3G50700 | 86.408 | 103 | 13 | 1 | 22 | 124 | 34 | 135 | 1.37e-64 | 202 |
| MS.gene014532.t1 | AT5G66730 | 86.408 | 103 | 13 | 1 | 22 | 124 | 32 | 133 | 2.58e-63 | 200 |
| MS.gene014532.t1 | AT3G13810 | 83.495 | 103 | 17 | 0 | 22 | 124 | 51 | 153 | 5.01e-63 | 199 |
| MS.gene014532.t1 | AT3G13810 | 83.495 | 103 | 17 | 0 | 22 | 124 | 65 | 167 | 5.74e-63 | 200 |
| MS.gene014532.t1 | AT3G13810 | 83.495 | 103 | 17 | 0 | 22 | 124 | 70 | 172 | 6.50e-63 | 200 |
| MS.gene014532.t1 | AT3G13810 | 83.495 | 103 | 17 | 0 | 22 | 124 | 70 | 172 | 6.50e-63 | 200 |
| MS.gene014532.t1 | AT1G03840 | 80.000 | 110 | 21 | 1 | 15 | 124 | 34 | 142 | 1.72e-61 | 196 |
| MS.gene014532.t1 | AT4G02670 | 82.524 | 103 | 18 | 0 | 22 | 124 | 53 | 155 | 6.73e-61 | 192 |
| MS.gene014532.t1 | AT4G02670 | 82.524 | 103 | 18 | 0 | 22 | 124 | 55 | 157 | 7.38e-61 | 192 |
| MS.gene014532.t1 | AT1G55110 | 80.583 | 103 | 20 | 0 | 22 | 124 | 63 | 165 | 2.01e-60 | 192 |
| MS.gene014532.t1 | AT1G55110 | 80.583 | 103 | 20 | 0 | 22 | 124 | 63 | 165 | 2.01e-60 | 192 |
| MS.gene014532.t1 | AT1G55110 | 80.583 | 103 | 20 | 0 | 22 | 124 | 63 | 165 | 2.01e-60 | 192 |
| MS.gene014532.t1 | AT5G60470 | 72.358 | 123 | 30 | 2 | 2 | 124 | 28 | 146 | 3.41e-60 | 191 |
| MS.gene014532.t1 | AT1G14580 | 76.364 | 110 | 25 | 1 | 15 | 124 | 46 | 154 | 3.57e-60 | 191 |
| MS.gene014532.t1 | AT1G14580 | 76.364 | 110 | 25 | 1 | 15 | 124 | 46 | 154 | 3.57e-60 | 191 |
| MS.gene014532.t1 | AT5G60470 | 72.358 | 123 | 30 | 2 | 2 | 124 | 24 | 142 | 3.95e-60 | 191 |
| MS.gene014532.t1 | AT1G14580 | 76.364 | 110 | 25 | 1 | 15 | 124 | 60 | 168 | 5.47e-60 | 191 |
| MS.gene014532.t1 | AT3G45260 | 76.923 | 104 | 24 | 0 | 21 | 124 | 38 | 141 | 9.43e-59 | 187 |
| MS.gene014532.t1 | AT3G45260 | 76.923 | 104 | 24 | 0 | 21 | 124 | 38 | 141 | 9.43e-59 | 187 |
| MS.gene014532.t1 | AT2G02080 | 75.455 | 110 | 25 | 2 | 15 | 124 | 48 | 155 | 1.02e-58 | 189 |
| MS.gene014532.t1 | AT2G02080 | 75.455 | 110 | 25 | 2 | 15 | 124 | 48 | 155 | 1.02e-58 | 189 |
| MS.gene014532.t1 | AT2G02080 | 75.455 | 110 | 25 | 2 | 15 | 124 | 48 | 155 | 1.02e-58 | 189 |
| MS.gene014532.t1 | AT2G02080 | 75.455 | 110 | 25 | 2 | 15 | 124 | 48 | 155 | 1.02e-58 | 189 |
| MS.gene014532.t1 | AT5G44160 | 78.505 | 107 | 22 | 1 | 18 | 124 | 33 | 138 | 1.11e-58 | 187 |
| MS.gene014532.t1 | AT1G03840 | 78.182 | 110 | 21 | 2 | 15 | 124 | 34 | 140 | 3.16e-58 | 187 |
| MS.gene014532.t1 | AT5G03150 | 75.962 | 104 | 25 | 0 | 21 | 124 | 52 | 155 | 1.02e-57 | 186 |
| MS.gene014532.t1 | AT3G13810 | 84.783 | 92 | 14 | 0 | 33 | 124 | 68 | 159 | 4.62e-57 | 184 |
| MS.gene014532.t1 | AT3G13810 | 84.783 | 92 | 14 | 0 | 33 | 124 | 82 | 173 | 5.60e-57 | 184 |
| MS.gene014532.t1 | AT2G02070 | 78.641 | 103 | 21 | 1 | 22 | 124 | 52 | 153 | 8.03e-56 | 183 |
| MS.gene014532.t1 | AT2G02070 | 78.641 | 103 | 21 | 1 | 22 | 124 | 52 | 153 | 8.03e-56 | 183 |
| MS.gene014532.t1 | AT5G60470 | 64.800 | 125 | 38 | 2 | 6 | 124 | 13 | 137 | 5.89e-53 | 172 |
| MS.gene014532.t1 | AT5G44160 | 78.495 | 93 | 19 | 1 | 32 | 124 | 8 | 99 | 2.85e-51 | 167 |
| MS.gene014532.t1 | AT2G01940 | 62.727 | 110 | 41 | 0 | 15 | 124 | 37 | 146 | 1.38e-48 | 161 |
| MS.gene014532.t1 | AT2G01940 | 62.727 | 110 | 41 | 0 | 15 | 124 | 37 | 146 | 1.51e-48 | 160 |
| MS.gene014532.t1 | AT1G68130 | 62.963 | 108 | 40 | 0 | 17 | 124 | 36 | 143 | 1.95e-48 | 160 |
| MS.gene014532.t1 | AT1G25250 | 59.649 | 114 | 45 | 1 | 12 | 124 | 23 | 136 | 1.48e-47 | 157 |
| MS.gene014532.t1 | AT1G25250 | 65.625 | 96 | 32 | 1 | 30 | 124 | 18 | 113 | 1.02e-44 | 149 |
| MS.gene014532.t1 | AT2G02080 | 75.949 | 79 | 18 | 1 | 46 | 124 | 1 | 78 | 6.32e-43 | 146 |
| MS.gene014532.t1 | AT2G02080 | 75.949 | 79 | 18 | 1 | 46 | 124 | 1 | 78 | 6.32e-43 | 146 |
| MS.gene014532.t1 | AT1G68130 | 64.912 | 57 | 20 | 0 | 68 | 124 | 1 | 57 | 3.46e-22 | 89.4 |
| MS.gene014532.t1 | AT2G01940 | 63.158 | 57 | 21 | 0 | 68 | 124 | 1 | 57 | 2.62e-21 | 87.4 |
| MS.gene014532.t1 | AT1G25250 | 63.793 | 58 | 20 | 1 | 68 | 124 | 1 | 58 | 9.12e-21 | 85.5 |
Find 31 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGTTGCTAGTAAAGTTT+TGG | 0.275277 | 3.4:+96737640 | None:intergenic |
| TTATATACACCTGGCATTCC+AGG | 0.339087 | 3.4:+96737858 | None:intergenic |
| TTTCGTCGTTCGCTCGCTGC+CGG | 0.456453 | 3.4:+96737528 | None:intergenic |
| AAAGCGATTACTTCAGCGTC+TGG | 0.457409 | 3.4:+96737668 | None:intergenic |
| CGAAACCTCCCTGGAATGCC+AGG | 0.466668 | 3.4:-96737867 | MS.gene014532:intron |
| ACAACATGCCGTGGAAGCTC+CGG | 0.473951 | 3.4:-96737547 | MS.gene014532:CDS |
| TGGTGAACGCAGGAAGGCTC+AGG | 0.513816 | 3.4:+96737479 | None:intergenic |
| TACAGATCTCGCATATGAAA+CGG | 0.517803 | 3.4:+96737618 | None:intergenic |
| CTGTAACAAAGGATTCCAAC+GGG | 0.530824 | 3.4:-96737600 | MS.gene014532:CDS |
| ATATGCGAGATCTGTAACAA+AGG | 0.533818 | 3.4:-96737611 | MS.gene014532:CDS |
| TGTGTCCTCGCCGGTGAAGT+TGG | 0.538471 | 3.4:+96737567 | None:intergenic |
| GCTCGCTGCCGGAGCTTCCA+CGG | 0.538801 | 3.4:+96737539 | None:intergenic |
| TATATACACCTGGCATTCCA+GGG | 0.541617 | 3.4:+96737859 | None:intergenic |
| GTTGGAGATTCTGTTCCCGT+TGG | 0.557245 | 3.4:+96737585 | None:intergenic |
| AGGTCACCGAGTGCTCTTGA+AGG | 0.557256 | 3.4:+96737452 | None:intergenic |
| CACAATCCTTCAAGAGCACT+CGG | 0.561083 | 3.4:-96737458 | MS.gene014532:CDS |
| AACAGAATCTCCAACTTCAC+CGG | 0.564489 | 3.4:-96737577 | MS.gene014532:CDS |
| ACGGCATGTTGTGTCCTCGC+CGG | 0.565267 | 3.4:+96737558 | None:intergenic |
| AGAGCACTCGGTGACCTCAC+AGG | 0.584571 | 3.4:-96737446 | MS.gene014532:CDS |
| GGCGAGGACACAACATGCCG+TGG | 0.596259 | 3.4:-96737556 | MS.gene014532:CDS |
| TGAAGGATTGTGGTGAACGC+AGG | 0.600303 | 3.4:+96737469 | None:intergenic |
| GACAAACGTACACTTTCTTG+CGG | 0.615008 | 3.4:+96737501 | None:intergenic |
| AAACGTACACTTTCTTGCGG+AGG | 0.628231 | 3.4:+96737504 | None:intergenic |
| TCTGTAACAAAGGATTCCAA+CGG | 0.628991 | 3.4:-96737601 | MS.gene014532:CDS |
| GGATTGTGGTGAACGCAGGA+AGG | 0.629061 | 3.4:+96737473 | None:intergenic |
| GATCTCGCATATGAAACGGT+TGG | 0.639364 | 3.4:+96737622 | None:intergenic |
| GGTGTAGTAGAAAGTGCCAT+TGG | 0.644739 | 3.4:+96737909 | None:intergenic |
| GTAGTAGAAAGTGCCATTGG+TGG | 0.678545 | 3.4:+96737912 | None:intergenic |
| CGAGTGCTCTTGAAGGATTG+TGG | 0.681807 | 3.4:+96737459 | None:intergenic |
| AATCTCCAACTTCACCGGCG+AGG | 0.689245 | 3.4:-96737572 | MS.gene014532:CDS |
| ATACACCTGGCATTCCAGGG+AGG | 0.741719 | 3.4:+96737862 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATTTATTTATATAATAG+TGG | + | chr3.4:96737403-96737422 | None:intergenic | 10.0% |
| !! | GATATTTATTTATATACACC+TGG | + | chr3.4:96737381-96737400 | None:intergenic | 20.0% |
| GTGATTTGAAAGTTCATATG+AGG | - | chr3.4:96737884-96737903 | MS.gene014532:CDS | 30.0% | |
| ! | AGAGAGTATAAATGTGATTG+TGG | - | chr3.4:96737919-96737938 | MS.gene014532:CDS | 30.0% |
| ! | TTGGTTGCTAGTAAAGTTTT+GGG | + | chr3.4:96737589-96737608 | None:intergenic | 30.0% |
| AAAGTTCATATGAGGAACTG+TGG | - | chr3.4:96737892-96737911 | MS.gene014532:CDS | 35.0% | |
| ATATGCGAGATCTGTAACAA+AGG | - | chr3.4:96737616-96737635 | MS.gene014532:CDS | 35.0% | |
| GAAAAATACTTCACCACCAA+TGG | - | chr3.4:96737302-96737321 | MS.gene014532:intron | 35.0% | |
| TACAGATCTCGCATATGAAA+CGG | + | chr3.4:96737612-96737631 | None:intergenic | 35.0% | |
| TCTGTAACAAAGGATTCCAA+CGG | - | chr3.4:96737626-96737645 | MS.gene014532:CDS | 35.0% | |
| ! | AAGCACTTTTGTAGAAAGCA+CGG | - | chr3.4:96737811-96737830 | MS.gene014532:intron | 35.0% |
| ! | GTTGGTTGCTAGTAAAGTTT+TGG | + | chr3.4:96737590-96737609 | None:intergenic | 35.0% |
| !!! | AGGTTTCGCTTCTTTTTTGA+CGG | + | chr3.4:96737348-96737367 | None:intergenic | 35.0% |
| !!! | GAAGTATTTTTCTGAGGTTC+TGG | + | chr3.4:96737294-96737313 | None:intergenic | 35.0% |
| AACAGAATCTCCAACTTCAC+CGG | - | chr3.4:96737650-96737669 | MS.gene014532:CDS | 40.0% | |
| CTGTAACAAAGGATTCCAAC+GGG | - | chr3.4:96737627-96737646 | MS.gene014532:CDS | 40.0% | |
| GACAAACGTACACTTTCTTG+CGG | + | chr3.4:96737729-96737748 | None:intergenic | 40.0% | |
| TATATACACCTGGCATTCCA+GGG | + | chr3.4:96737371-96737390 | None:intergenic | 40.0% | |
| TTATATACACCTGGCATTCC+AGG | + | chr3.4:96737372-96737391 | None:intergenic | 40.0% | |
| ! | ATTGTGGCACTGTGTTTTCT+AGG | - | chr3.4:96737935-96737954 | MS.gene014532:CDS | 40.0% |
| !! | GGTGGTGAAGTATTTTTCTG+AGG | + | chr3.4:96737300-96737319 | None:intergenic | 40.0% |
| !!! | TTTCGCTTCTTTTTTGACGG+TGG | + | chr3.4:96737345-96737364 | None:intergenic | 40.0% |
| AAAAAGAAGCGAAACCTCCC+TGG | - | chr3.4:96737351-96737370 | MS.gene014532:intron | 45.0% | |
| AAACGTACACTTTCTTGCGG+AGG | + | chr3.4:96737726-96737745 | None:intergenic | 45.0% | |
| AAAGCGATTACTTCAGCGTC+TGG | + | chr3.4:96737562-96737581 | None:intergenic | 45.0% | |
| GATCTCGCATATGAAACGGT+TGG | + | chr3.4:96737608-96737627 | None:intergenic | 45.0% | |
| ! | GAAAGCACGGTGAGAAGAAA+TGG | - | chr3.4:96737824-96737843 | MS.gene014532:intron | 45.0% |
| !! | CACAATCCTTCAAGAGCACT+CGG | - | chr3.4:96737769-96737788 | MS.gene014532:intron | 45.0% |
| !! | GGTGTAGTAGAAAGTGCCAT+TGG | + | chr3.4:96737321-96737340 | None:intergenic | 45.0% |
| !! | GTAGTAGAAAGTGCCATTGG+TGG | + | chr3.4:96737318-96737337 | None:intergenic | 45.0% |
| !!! | AGTGCTTTTTCACTCCTGTG+AGG | + | chr3.4:96737798-96737817 | None:intergenic | 45.0% |
| CGAGTGCTCTTGAAGGATTG+TGG | + | chr3.4:96737771-96737790 | None:intergenic | 50.0% | |
| GTTGGAGATTCTGTTCCCGT+TGG | + | chr3.4:96737645-96737664 | None:intergenic | 50.0% | |
| TCAGCGTCTGGATCTGCAAA+CGG | + | chr3.4:96737550-96737569 | None:intergenic | 50.0% | |
| TGAAGGATTGTGGTGAACGC+AGG | + | chr3.4:96737761-96737780 | None:intergenic | 50.0% | |
| !!! | CGCTTCTTTTTTGACGGTGG+CGG | + | chr3.4:96737342-96737361 | None:intergenic | 50.0% |
| AATCTCCAACTTCACCGGCG+AGG | - | chr3.4:96737655-96737674 | MS.gene014532:CDS | 55.0% | |
| ACAACATGCCGTGGAAGCTC+CGG | - | chr3.4:96737680-96737699 | MS.gene014532:intron | 55.0% | |
| AGGTCACCGAGTGCTCTTGA+AGG | + | chr3.4:96737778-96737797 | None:intergenic | 55.0% | |
| ATACACCTGGCATTCCAGGG+AGG | + | chr3.4:96737368-96737387 | None:intergenic | 55.0% | |
| GGATTGTGGTGAACGCAGGA+AGG | + | chr3.4:96737757-96737776 | None:intergenic | 55.0% | |
| ACGGCATGTTGTGTCCTCGC+CGG | + | chr3.4:96737672-96737691 | None:intergenic | 60.0% | |
| CGAAACCTCCCTGGAATGCC+AGG | - | chr3.4:96737360-96737379 | MS.gene014532:intron | 60.0% | |
| TGGTGAACGCAGGAAGGCTC+AGG | + | chr3.4:96737751-96737770 | None:intergenic | 60.0% | |
| TGTGTCCTCGCCGGTGAAGT+TGG | + | chr3.4:96737663-96737682 | None:intergenic | 60.0% | |
| TTTCGTCGTTCGCTCGCTGC+CGG | + | chr3.4:96737702-96737721 | None:intergenic | 60.0% | |
| ! | AGAGCACTCGGTGACCTCAC+AGG | - | chr3.4:96737781-96737800 | MS.gene014532:intron | 60.0% |
| GGCGAGGACACAACATGCCG+TGG | - | chr3.4:96737671-96737690 | MS.gene014532:CDS | 65.0% | |
| GCTCGCTGCCGGAGCTTCCA+CGG | + | chr3.4:96737691-96737710 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 96737288 | 96737961 | 96737288 | ID=MS.gene014532 |
| chr3.4 | mRNA | 96737288 | 96737961 | 96737288 | ID=MS.gene014532.t1;Parent=MS.gene014532 |
| chr3.4 | exon | 96737868 | 96737961 | 96737868 | ID=MS.gene014532.t1.exon1;Parent=MS.gene014532.t1 |
| chr3.4 | CDS | 96737868 | 96737961 | 96737868 | ID=cds.MS.gene014532.t1;Parent=MS.gene014532.t1 |
| chr3.4 | exon | 96737417 | 96737692 | 96737417 | ID=MS.gene014532.t1.exon2;Parent=MS.gene014532.t1 |
| chr3.4 | CDS | 96737417 | 96737692 | 96737417 | ID=cds.MS.gene014532.t1;Parent=MS.gene014532.t1 |
| chr3.4 | exon | 96737288 | 96737292 | 96737288 | ID=MS.gene014532.t1.exon3;Parent=MS.gene014532.t1 |
| chr3.4 | CDS | 96737288 | 96737292 | 96737288 | ID=cds.MS.gene014532.t1;Parent=MS.gene014532.t1 |
| Gene Sequence |
| Protein sequence |
